Rroxscaffold_2G00083950

Lamin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008382
Physical Location & Seq
Reverse (-)
6482887 .. 6484392
1506 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00083950.1

Sequence Viewer

Length: 579 bp
ATGGCGGCTGTGGTGCTTTTGGCCTTGGTTTTGGTAATGGTTCCAGAAGCCTCAGCTGCTCGTATTACTGTGGGAGGTAACAAGGGCTGGTCTCCCAATGTTAACTACACAATTTGGGCGCAAGACAAGCACTTCTACAATGGAGATTGGCTCTATCTGCAACTGGTGCTATTTTTGCTTTTAGTTTTTGCTGTGAAGAAATCCAATGCCTTATTACCTGTGCTGCTATACAGCATTTTTGTGTATGATAGGAACCAGCAGAATATCCTGGAGGTGAACAAGACAAACTATGAGACATGCAATTCAGATCATCCACTTCACAATTGGACTACAGGAGTTGGAAGAGATGTGGTTCCACTAAATGTGACCAGGCACTACTATTTCTTGAGTGGCAATGGCTTCTGCTACAGTGGCATGAAGATGGCTATTCAGGTTGAAAAAATACCTCCACCTCCCAAGGCTGCTCCGGTGGTGAGCAAGGGCAGTGCTCCATTAGCGCCTGTTTTTAGATCACAATTTGTGTTGCCAGCCATTTTTGCTATTGGTGCAGTTTGGGATGCATTTCTTAGGTTCTGCTAG

Protein Analysis

192

Amino Acids

21.39

Weight (kDa)

9.15

Isoelectric Point (pI)

41.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 79 - 139 1.8e-12 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 5
AgsI TTSAA 1 cut(s) 437
AjnI CCWGG 2 cut(s) 267, 368
AluBI AGCT 1 cut(s) 56
AluI AGCT 1 cut(s) 56
Alw21I GWGCWC 1 cut(s) 490
Alw26I GTCTC 2 cut(s) 96, 287
AoxI GGCC 1 cut(s) 21
ApeKI GCWGC 3 cut(s) 56, 223, 461
AspLEI GCGC 2 cut(s) 121, 499
AsuHPI GGTGA 2 cut(s) 286, 484
Bbv12I GWGCWC 1 cut(s) 490
BbvCI CCTCAGC 1 cut(s) 52
BbvI GCAGC 3 cut(s) 43, 210, 448
BccI CCATC 1 cut(s) 415
BciT130I CCWGG 2 cut(s) 269, 370
BcoDI GTCTC 2 cut(s) 96, 287
BfaI CTAG 1 cut(s) 577
BfmI CTRYAG 2 cut(s) 330, 406
BfoI RGCGCY 1 cut(s) 500
BisI GCNGC 4 cut(s) 6, 57, 224, 462
BlsI GCNGC 4 cut(s) 7, 58, 225, 463
Bme1390I CCNGG 2 cut(s) 269, 370
BmiI GGNNCC 3 cut(s) 42, 254, 354
BmrFI CCNGG 2 cut(s) 269, 370
BmsI GCATC 1 cut(s) 547
BplI GAGNNNNNCTC 2 cut(s) 135, 167
BpmI CTGGAG 1 cut(s) 290
Bpu10I CCTNAGC 1 cut(s) 52
BpuEI CTTGAG 1 cut(s) 406
BsaI GGTCTC 1 cut(s) 96
BsaJI CCNNGG 2 cut(s) 24, 456
BsaWI WCCGGW 1 cut(s) 466
Bse1I ACTGG 1 cut(s) 168
Bse3DI GCAATG 1 cut(s) 400
BseBI CCWGG 2 cut(s) 269, 370
BseDI CCNNGG 2 cut(s) 24, 456
BseGI GGATG 2 cut(s) 310, 562
BseMI GCAATG 1 cut(s) 400
BseMII CTCAG 1 cut(s) 66
BseNI ACTGG 1 cut(s) 168
BseXI GCAGC 3 cut(s) 43, 210, 448
BsgI GTGCAG 1 cut(s) 567
BshFI GGCC 1 cut(s) 23
BsiHKAI GWGCWC 1 cut(s) 490
BsiSI CCGG 1 cut(s) 467
BsmAI GTCTC 2 cut(s) 96, 287
BsnI GGCC 1 cut(s) 23
Bso31I GGTCTC 1 cut(s) 96
Bsp1286I GDGCHC 1 cut(s) 490
Bsp143I GATC 2 cut(s) 307, 509
BspACI CCGC 1 cut(s) 5
BspANI GGCC 1 cut(s) 23
BspCNI CTCAG 1 cut(s) 65
BspLI GGNNCC 3 cut(s) 42, 254, 354
BspTNI GGTCTC 1 cut(s) 96
BsrDI GCAATG 1 cut(s) 400
BsrI ACTGG 1 cut(s) 168
BssECI CCNNGG 2 cut(s) 24, 456
BssMI GATC 2 cut(s) 307, 509
BssT1I CCWWGG 2 cut(s) 24, 456
Bst2UI CCWGG 2 cut(s) 269, 370
Bst4CI ACNGT 2 cut(s) 70, 410
Bst6I CTCTTC 1 cut(s) 337
BstAPI GCANNNNNTGC 1 cut(s) 166
BstC8I GCNNGC 1 cut(s) 528
BstDEI CTNAG 2 cut(s) 52, 566
BstF5I GGATG 2 cut(s) 310, 562
BstH2I RGCGCY 1 cut(s) 500
BstHHI GCGC 2 cut(s) 121, 499
BstKTI GATC 2 cut(s) 310, 512
BstMAI GTCTC 2 cut(s) 96, 287
BstMBI GATC 2 cut(s) 307, 509
BstMWI GCNNNNNNNGC 9 cut(s) 56, 127, 157, 166, 175, 411, 494, 536, 545
BstNI CCWGG 2 cut(s) 269, 370
BstNSI RCATGY 1 cut(s) 300
BstSCI CCNGG 2 cut(s) 267, 368
BstSFI CTRYAG 2 cut(s) 330, 406
BstV1I GCAGC 3 cut(s) 43, 210, 448
BsuRI GGCC 1 cut(s) 23
BtsCI GGATG 2 cut(s) 310, 562
BtsI GCAGTG 1 cut(s) 490
BtsIMutI CAGTG 2 cut(s) 415, 490
Cac8I GCNNGC 1 cut(s) 528
CfoI GCGC 2 cut(s) 121, 499
CviAII CATG 2 cut(s) 297, 415
DdeI CTNAG 2 cut(s) 52, 566
DpnI GATC 2 cut(s) 309, 511
DpnII GATC 2 cut(s) 307, 509
Eam1104I CTCTTC 1 cut(s) 337
EarI CTCTTC 1 cut(s) 337
Eco130I CCWWGG 2 cut(s) 24, 456
Eco31I GGTCTC 1 cut(s) 96
EcoRII CCWGG 2 cut(s) 267, 368
EcoT14I CCWWGG 2 cut(s) 24, 456
EcoT22I ATGCAT 1 cut(s) 562
ErhI CCWWGG 2 cut(s) 24, 456
FaeI CATG 2 cut(s) 300, 418
FaiI YATR 5 cut(s) 229, 246, 291, 298, 416
FatI CATG 2 cut(s) 296, 414
Fnu4HI GCNGC 4 cut(s) 6, 57, 224, 462
FokI GGATG 2 cut(s) 297, 569
Fsp4HI GCNGC 4 cut(s) 6, 57, 224, 462
FspBI CTAG 1 cut(s) 577
GlaI GCGC 2 cut(s) 120, 498
GluI GCNGC 4 cut(s) 6, 57, 224, 462
GsuI CTGGAG 1 cut(s) 290
HaeII RGCGCY 1 cut(s) 500
HaeIII GGCC 1 cut(s) 23
HapII CCGG 1 cut(s) 467
HhaI GCGC 2 cut(s) 121, 499
Hin1II CATG 2 cut(s) 300, 418
Hin6I GCGC 2 cut(s) 119, 497
HinP1I GCGC 2 cut(s) 119, 497
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HpaI GTTAAC 1 cut(s) 103
HpaII CCGG 1 cut(s) 467
HphI GGTGA 2 cut(s) 286, 484
Hpy166II GTNNAC 2 cut(s) 103, 277
Hpy188I TCNGA 1 cut(s) 307
Hpy188III TCNNGA 2 cut(s) 44, 385
Hpy8I GTNNAC 2 cut(s) 103, 277
HpyCH4III ACNGT 2 cut(s) 70, 410
HpyCH4V TGCA 4 cut(s) 160, 300, 548, 560
HpyF10VI GCNNNNNNNGC 9 cut(s) 56, 127, 157, 166, 175, 411, 494, 536, 545
HpyF3I CTNAG 2 cut(s) 52, 566
Hsp92II CATG 2 cut(s) 300, 418
HspAI GCGC 2 cut(s) 119, 497
KspAI GTTAAC 1 cut(s) 103
Kzo9I GATC 2 cut(s) 307, 509
LmnI GCTCC 2 cut(s) 469, 493
Lsp1109I GCAGC 3 cut(s) 43, 210, 448
LweI GCATC 1 cut(s) 547
MaeI CTAG 1 cut(s) 577
MaeIII GTNAC 2 cut(s) 77, 364
MalI GATC 2 cut(s) 309, 511
MboI GATC 2 cut(s) 307, 509
MboII GAAGA 3 cut(s) 208, 354, 430
MfeI CAATTG 1 cut(s) 322
MhlI GDGCHC 1 cut(s) 490
MluCI AATT 4 cut(s) 111, 301, 322, 515
MmeI TCCRAC 1 cut(s) 319
MnlI CCTC 5 cut(s) 61, 68, 265, 456, 462
Mph1103I ATGCAT 1 cut(s) 562
MseI TTAA 1 cut(s) 102
MslI CAYNNNNRTG 2 cut(s) 239, 419
MspA1I CMGCKG 1 cut(s) 56
MspI CCGG 1 cut(s) 467
MspR9I CCNGG 2 cut(s) 269, 370
MunI CAATTG 1 cut(s) 322
MvaI CCWGG 2 cut(s) 269, 370
MwoI GCNNNNNNNGC 9 cut(s) 56, 127, 157, 166, 175, 411, 494, 536, 545
NdeII GATC 2 cut(s) 307, 509
NlaIII CATG 2 cut(s) 300, 418
NlaIV GGNNCC 3 cut(s) 42, 254, 354
NmuCI GTSAC 1 cut(s) 364
NsiI ATGCAT 1 cut(s) 562
NspI RCATGY 1 cut(s) 300
PfoI TCCNGGA 1 cut(s) 267
PkrI GCNGC 4 cut(s) 7, 58, 225, 463
Psp6I CCWGG 2 cut(s) 267, 368
PspGI CCWGG 2 cut(s) 267, 368
PspN4I GGNNCC 3 cut(s) 42, 254, 354
PvuII CAGCTG 1 cut(s) 56
RseI CAYNNNNRTG 2 cut(s) 239, 419
SaqAI TTAA 1 cut(s) 102
SatI GCNGC 4 cut(s) 6, 57, 224, 462
Sau3AI GATC 2 cut(s) 307, 509
ScrFI CCNGG 2 cut(s) 269, 370
SduI GDGCHC 1 cut(s) 490
SetI ASST 8 cut(s) 58, 79, 220, 276, 435, 448, 454, 572
SfaNI GCATC 1 cut(s) 547
SfcI CTRYAG 2 cut(s) 330, 406
SmiMI CAYNNNNRTG 2 cut(s) 239, 419
SmlI CTYRAG 1 cut(s) 385
SmoI CTYRAG 1 cut(s) 385
Sse9I AATT 4 cut(s) 111, 301, 322, 515
SsiI CCGC 1 cut(s) 5
SspMI CTAG 1 cut(s) 577
StyD4I CCNGG 2 cut(s) 267, 368
StyI CCWWGG 2 cut(s) 24, 456
TaaI ACNGT 2 cut(s) 70, 410
TasI AATT 4 cut(s) 111, 301, 322, 515
TauI GCSGC 1 cut(s) 8
Tru1I TTAA 1 cut(s) 102
Tru9I TTAA 1 cut(s) 102
TscAI CASTG 2 cut(s) 415, 490
TseFI GTSAC 1 cut(s) 364
TseI GCWGC 3 cut(s) 56, 223, 461
Tsp45I GTSAC 1 cut(s) 364
TspDTI ATGAA 1 cut(s) 431
TspRI CASTG 2 cut(s) 415, 490
XceI RCATGY 1 cut(s) 300
XcmI CCANNNNNNNNNTGG 1 cut(s) 321
XspI CTAG 1 cut(s) 577
Zsp2I ATGCAT 1 cut(s) 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.