MD09G1057600.v1.1

Lamin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008382
Physical Location & Seq
Reverse (-)
3810911 .. 3812153
1243 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1057600.v1.1.491

Sequence Viewer

Length: 390 bp
ATGGAGAGAAAGGGAGCCATGGTGGTGGTAGTGCTTTTGTGCTTGGCAGTGGTGATCGAACTTCCAGAAGCATCAGCTACGAGATATTTAGTGGGAGGGAAAATGGGTTGGACCAGCAATGTCAACTATACTATTTGGGCTCAGGATAAGCACTTTTACAATGGAGACTGGCTCTTTTTTGTGTATGATAGGAACCAAAATGATGTTCTAGAGGTAAACCAGACAAACTATGTCTCGTGCAATGTTGAACATCCCCTTCACAACTGGACCACCGGAGCCGGAAGAGATATTGTTCCACTGAATGAGCCAAGGCACTACTACTTCATTAGTAGCAATGGGTTCTGCTACAGTGGCATGAAGATTGCCGTTAAAGTTGAAAACATGCCTTAG

Protein Analysis

130

Amino Acids

14.77

Weight (kDa)

6.04

Isoelectric Point (pI)

27.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 37 - 119 5.2e-23 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AgsI TTSAA 2 cut(s) 248, 377
AjuI GAANNNNNNNTTGG 2 cut(s) 189, 221
AluBI AGCT 1 cut(s) 77
AluI AGCT 1 cut(s) 77
Alw26I GTCTC 2 cut(s) 159, 238
AspS9I GGNCC 2 cut(s) 111, 267
AsuHPI GGTGA 1 cut(s) 64
AvaII GGWCC 2 cut(s) 111, 267
BanII GRGCYC 1 cut(s) 142
BauI CACGAG 1 cut(s) 235
BceAI ACGGC 1 cut(s) 350
BcoDI GTCTC 2 cut(s) 159, 238
BfaI CTAG 1 cut(s) 209
BfmI CTRYAG 1 cut(s) 346
Bme18I GGWCC 2 cut(s) 111, 267
BmgT120I GGNCC 2 cut(s) 111, 267
BmiI GGNNCC 3 cut(s) 16, 194, 277
BmsI GCATC 1 cut(s) 80
BplI GAGNNNNNCTC 2 cut(s) 156, 188
Bpu10I CCTNAGC 1 cut(s) 141
BsaJI CCNNGG 2 cut(s) 18, 308
BsaWI WCCGGW 1 cut(s) 272
BsaXI ACNNNNNCTCC 2 cut(s) 6, 36
Bse1I ACTGG 2 cut(s) 173, 269
Bse3DI GCAATG 3 cut(s) 124, 247, 340
BseDI CCNNGG 2 cut(s) 18, 308
BseGI GGATG 1 cut(s) 250
BseMI GCAATG 3 cut(s) 124, 247, 340
BseMII CTCAG 1 cut(s) 155
BseNI ACTGG 2 cut(s) 173, 269
BsiSI CCGG 2 cut(s) 273, 279
BsmAI GTCTC 2 cut(s) 159, 238
Bsp1286I GDGCHC 1 cut(s) 142
Bsp143I GATC 1 cut(s) 54
Bsp19I CCATGG 1 cut(s) 18
BspCNI CTCAG 1 cut(s) 154
BspLI GGNNCC 3 cut(s) 16, 194, 277
BsrDI GCAATG 3 cut(s) 124, 247, 340
BsrI ACTGG 2 cut(s) 173, 269
BssECI CCNNGG 2 cut(s) 18, 308
BssMI GATC 1 cut(s) 54
BssSI CACGAG 1 cut(s) 235
BssT1I CCWWGG 2 cut(s) 18, 308
Bst2BI CACGAG 1 cut(s) 235
Bst4CI ACNGT 1 cut(s) 350
Bst6I CTCTTC 1 cut(s) 277
BstDEI CTNAG 2 cut(s) 141, 387
BstDSI CCRYGG 1 cut(s) 18
BstF5I GGATG 1 cut(s) 250
BstKTI GATC 1 cut(s) 57
BstMAI GTCTC 2 cut(s) 159, 238
BstMBI GATC 1 cut(s) 54
BstMWI GCNNNNNNNGC 1 cut(s) 351
BstNSI RCATGY 1 cut(s) 385
BstSFI CTRYAG 1 cut(s) 346
BstXI CCANNNNNNTGG 1 cut(s) 25
BtgI CCRYGG 1 cut(s) 18
BtsCI GGATG 1 cut(s) 250
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 3 cut(s) 54, 296, 355
Cfr13I GGNCC 2 cut(s) 111, 267
CviAII CATG 3 cut(s) 19, 355, 382
CviJI RGCY 6 cut(s) 17, 77, 140, 172, 278, 307
CviKI_1 RGCY 6 cut(s) 17, 77, 140, 172, 278, 307
DdeI CTNAG 2 cut(s) 141, 387
DpnI GATC 1 cut(s) 56
DpnII GATC 1 cut(s) 54
Eam1104I CTCTTC 1 cut(s) 277
EarI CTCTTC 1 cut(s) 277
Eco130I CCWWGG 2 cut(s) 18, 308
Eco24I GRGCYC 1 cut(s) 142
Eco47I GGWCC 2 cut(s) 111, 267
EcoT14I CCWWGG 2 cut(s) 18, 308
EcoT38I GRGCYC 1 cut(s) 142
ErhI CCWWGG 2 cut(s) 18, 308
FaeI CATG 3 cut(s) 22, 358, 385
FaiI YATR 6 cut(s) 20, 129, 186, 231, 356, 383
FatI CATG 3 cut(s) 18, 354, 381
FokI GGATG 1 cut(s) 237
FriOI GRGCYC 1 cut(s) 142
FspBI CTAG 1 cut(s) 209
HapII CCGG 2 cut(s) 273, 279
Hin1II CATG 3 cut(s) 22, 358, 385
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HpaII CCGG 2 cut(s) 273, 279
HphI GGTGA 1 cut(s) 64
Hpy166II GTNNAC 2 cut(s) 124, 217
Hpy188III TCNNGA 3 cut(s) 65, 143, 209
Hpy8I GTNNAC 2 cut(s) 124, 217
HpyAV CCTTC 1 cut(s) 266
HpyCH4III ACNGT 1 cut(s) 350
HpyCH4V TGCA 1 cut(s) 240
HpyF10VI GCNNNNNNNGC 1 cut(s) 351
HpyF3I CTNAG 2 cut(s) 141, 387
Hsp92II CATG 3 cut(s) 22, 358, 385
Kzo9I GATC 1 cut(s) 54
LmnI GCTCC 2 cut(s) 14, 275
LpnPI CCDG 8 cut(s) 78, 127, 128, 154, 233, 250, 286, 292
LweI GCATC 1 cut(s) 80
MaeI CTAG 1 cut(s) 209
MalI GATC 1 cut(s) 56
MboI GATC 1 cut(s) 54
MboII GAAGA 2 cut(s) 294, 370
MhlI GDGCHC 1 cut(s) 142
MmeI TCCRAC 1 cut(s) 89
MnlI CCTC 2 cut(s) 89, 205
MseI TTAA 1 cut(s) 369
MslI CAYNNNNRTG 1 cut(s) 23
MspI CCGG 2 cut(s) 273, 279
MwoI GCNNNNNNNGC 1 cut(s) 351
NcoI CCATGG 1 cut(s) 18
NdeII GATC 1 cut(s) 54
NlaIII CATG 3 cut(s) 22, 358, 385
NlaIV GGNNCC 3 cut(s) 16, 194, 277
NspI RCATGY 1 cut(s) 385
PspN4I GGNNCC 3 cut(s) 16, 194, 277
PspPI GGNCC 2 cut(s) 111, 267
RseI CAYNNNNRTG 1 cut(s) 23
SaqAI TTAA 1 cut(s) 369
Sau3AI GATC 1 cut(s) 54
Sau96I GGNCC 2 cut(s) 111, 267
SduI GDGCHC 1 cut(s) 142
SetI ASST 2 cut(s) 79, 216
SfaNI GCATC 1 cut(s) 80
SfcI CTRYAG 1 cut(s) 346
SinI GGWCC 2 cut(s) 111, 267
SmiMI CAYNNNNRTG 1 cut(s) 23
SspMI CTAG 1 cut(s) 209
StyI CCWWGG 2 cut(s) 18, 308
TaaI ACNGT 1 cut(s) 350
TaqI TCGA 1 cut(s) 57
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 3 cut(s) 54, 303, 355
TspDTI ATGAA 2 cut(s) 313, 371
TspRI CASTG 3 cut(s) 54, 303, 355
VpaK11BI GGWCC 2 cut(s) 111, 267
XbaI TCTAGA 1 cut(s) 208
XceI RCATGY 1 cut(s) 385
XspI CTAG 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.