Rorug02G0526500

Lamin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008382
Physical Location & Seq
Reverse (-)
65672152 .. 65673365
1214 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0526500.1

Sequence Viewer

Length: 537 bp
ATGGCATTAAACTTGATACCCAAGTTACTGTTGGTCCTGGTTCTATCCATGACTACATTTTCATCCAAAGCCAGTAGCAGTAAAGAGTACAAAGAAACCCACATGTCCATGTACCTTCAGGACTTCCTTACCGGTCCAAATGTCACAGATATACCAGTTGCGGGTGTCGCTGGAAAGCTTTGGGCCTTCAATCAATTTGGAACAGTTTATGTCAATGACAACCCTCTCACCGAAGGCCCAAGCCCACAATCGGCAGAGGTGGGCCGAGCCCCAGGTATTTTTGTGGCTTCAGCACTGGACGGATCTAGTGGCCTTGTTGCGTTTTCAGTAGTGTTCACAAACAAGGAGTACAATGGCAGCACCATAGAGATACTAGGAAACAGTAAAATACTGGACCCGGTTAGAGAGCTTTCGGTGGCATCCGGTACCGGAAAGTTTCGGTTTGCTAGAGGGTACGCTACGCTTGAAACCTATTTTCTGGACATTCCGAGAGCATACTCGATCGTACGACTCAACATAACAGTGGAACACAAGTAG

Protein Analysis

178

Amino Acids

19.28

Weight (kDa)

6.73

Isoelectric Point (pI)

33.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 33 - 175 1.7e-44 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 425
AccB1I GGYRCC 1 cut(s) 425
AciI CCGC 1 cut(s) 161
AclWI GGATC 1 cut(s) 310
AcuI CTGAAG 2 cut(s) 101, 273
AfaI GTAC 6 cut(s) 89, 113, 350, 427, 455, 507
AfiI CCNNNNNNNGG 2 cut(s) 161, 250
AflIII ACRYGT 1 cut(s) 102
AgeI ACCGGT 1 cut(s) 131
AgsI TTSAA 2 cut(s) 190, 467
AjnI CCWGG 2 cut(s) 36, 271
AluBI AGCT 2 cut(s) 178, 409
AluI AGCT 2 cut(s) 178, 409
AlwI GGATC 1 cut(s) 310
AoxI GGCC 4 cut(s) 183, 235, 262, 310
ApeKI GCWGC 1 cut(s) 357
AsiGI ACCGGT 1 cut(s) 131
Asp718I GGTACC 1 cut(s) 425
AspS9I GGNCC 6 cut(s) 34, 134, 183, 236, 262, 394
AsuC2I CCSGG 1 cut(s) 398
AsuHPI GGTGA 1 cut(s) 220
AvaII GGWCC 3 cut(s) 34, 134, 394
BaeI ACNNNNGTAYC 2 cut(s) 8, 41
BanI GGYRCC 1 cut(s) 425
BanII GRGCYC 1 cut(s) 271
BbvI GCAGC 1 cut(s) 369
BciT130I CCWGG 2 cut(s) 38, 273
BcnI CCSGG 1 cut(s) 398
BfaI CTAG 3 cut(s) 306, 374, 447
BisI GCNGC 1 cut(s) 358
BlsI GCNGC 1 cut(s) 359
Bme1390I CCNGG 3 cut(s) 38, 273, 398
Bme18I GGWCC 3 cut(s) 34, 134, 394
BmgT120I GGNCC 6 cut(s) 34, 134, 183, 236, 262, 394
BmiI GGNNCC 2 cut(s) 396, 427
BmrFI CCNGG 3 cut(s) 38, 273, 398
BmsI GCATC 1 cut(s) 428
BpuMI CCSGG 1 cut(s) 398
BsaJI CCNNGG 1 cut(s) 271
BsaWI WCCGGW 3 cut(s) 131, 422, 428
Bsc4I CCNNNNNNNGG 2 cut(s) 161, 250
Bse118I RCCGGY 1 cut(s) 131
Bse1I ACTGG 4 cut(s) 72, 155, 300, 396
BseBI CCWGG 2 cut(s) 38, 273
BseDI CCNNGG 1 cut(s) 271
BseGI GGATG 2 cut(s) 62, 419
BseLI CCNNNNNNNGG 2 cut(s) 161, 250
BseNI ACTGG 4 cut(s) 72, 155, 300, 396
BseXI GCAGC 1 cut(s) 369
Bsh1285I CGRYCG 1 cut(s) 504
BshFI GGCC 4 cut(s) 185, 237, 264, 312
BshNI GGYRCC 1 cut(s) 425
BshTI ACCGGT 1 cut(s) 131
BsiEI CGRYCG 1 cut(s) 504
BsiSI CCGG 4 cut(s) 132, 398, 423, 429
BsiWI CGTACG 1 cut(s) 505
BslI CCNNNNNNNGG 2 cut(s) 161, 250
BsnI GGCC 4 cut(s) 185, 237, 264, 312
Bsp1286I GDGCHC 1 cut(s) 271
Bsp143I GATC 2 cut(s) 302, 501
BspACI CCGC 1 cut(s) 161
BspANI GGCC 4 cut(s) 185, 237, 264, 312
BspLI GGNNCC 2 cut(s) 396, 427
BspPI GGATC 1 cut(s) 310
BspT107I GGYRCC 1 cut(s) 425
BsrFI RCCGGY 1 cut(s) 131
BsrI ACTGG 4 cut(s) 72, 155, 300, 396
BssAI RCCGGY 1 cut(s) 131
BssECI CCNNGG 1 cut(s) 271
BssMI GATC 2 cut(s) 302, 501
Bst2UI CCWGG 2 cut(s) 38, 273
Bst4CI ACNGT 4 cut(s) 30, 205, 383, 523
BstF5I GGATG 2 cut(s) 62, 419
BstKTI GATC 2 cut(s) 305, 504
BstMBI GATC 2 cut(s) 302, 501
BstMCI CGRYCG 1 cut(s) 504
BstMWI GCNNNNNNNGC 1 cut(s) 167
BstNI CCWGG 2 cut(s) 38, 273
BstNSI RCATGY 1 cut(s) 106
BstSCI CCNGG 3 cut(s) 36, 271, 396
BstV1I GCAGC 1 cut(s) 369
BstX2I RGATCY 1 cut(s) 302
BstYI RGATCY 1 cut(s) 302
BsuRI GGCC 4 cut(s) 185, 237, 264, 312
BtsCI GGATG 2 cut(s) 62, 419
BtsIMutI CAGTG 2 cut(s) 293, 528
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 6 cut(s) 34, 134, 183, 236, 262, 394
Csp6I GTAC 6 cut(s) 88, 112, 349, 426, 454, 506
CspAI ACCGGT 1 cut(s) 131
CviAII CATG 3 cut(s) 49, 103, 109
CviQI GTAC 6 cut(s) 88, 112, 349, 426, 454, 506
DpnI GATC 2 cut(s) 304, 503
DpnII GATC 2 cut(s) 302, 501
Eco24I GRGCYC 1 cut(s) 271
Eco47I GGWCC 3 cut(s) 34, 134, 394
Eco57I CTGAAG 2 cut(s) 101, 273
EcoRII CCWGG 2 cut(s) 36, 271
EcoT38I GRGCYC 1 cut(s) 271
FaeI CATG 3 cut(s) 52, 106, 112
FaiI YATR 8 cut(s) 50, 104, 110, 152, 210, 365, 496, 518
FatI CATG 3 cut(s) 48, 102, 108
FauI CCCGC 1 cut(s) 154
Fnu4HI GCNGC 1 cut(s) 358
FokI GGATG 2 cut(s) 49, 406
FriOI GRGCYC 1 cut(s) 271
Fsp4HI GCNGC 1 cut(s) 358
FspBI CTAG 3 cut(s) 306, 374, 447
GluI GCNGC 1 cut(s) 358
HaeIII GGCC 4 cut(s) 185, 237, 264, 312
HapII CCGG 4 cut(s) 132, 398, 423, 429
Hin1II CATG 3 cut(s) 52, 106, 112
HindIII AAGCTT 1 cut(s) 176
HinfI GANTC 1 cut(s) 510
HpaII CCGG 4 cut(s) 132, 398, 423, 429
HphI GGTGA 1 cut(s) 220
Hpy166II GTNNAC 1 cut(s) 336
Hpy188I TCNGA 1 cut(s) 489
Hpy188III TCNNGA 2 cut(s) 119, 479
Hpy8I GTNNAC 1 cut(s) 336
HpyAV CCTTC 3 cut(s) 125, 196, 227
HpyCH4III ACNGT 4 cut(s) 30, 205, 383, 523
HpyF10VI GCNNNNNNNGC 1 cut(s) 167
Hsp92II CATG 3 cut(s) 52, 106, 112
KpnI GGTACC 1 cut(s) 429
Kzo9I GATC 2 cut(s) 302, 501
Lsp1109I GCAGC 1 cut(s) 369
LweI GCATC 1 cut(s) 428
MaeI CTAG 3 cut(s) 306, 374, 447
MaeIII GTNAC 2 cut(s) 24, 142
MalI GATC 2 cut(s) 304, 503
MboI GATC 2 cut(s) 302, 501
MflI RGATCY 1 cut(s) 302
MhlI GDGCHC 1 cut(s) 271
MluCI AATT 1 cut(s) 194
MlyI GAGTC 1 cut(s) 504
MnlI CCTC 3 cut(s) 234, 250, 443
MseI TTAA 1 cut(s) 8
MslI CAYNNNNRTG 2 cut(s) 107, 521
MspI CCGG 4 cut(s) 132, 398, 423, 429
MspR9I CCNGG 3 cut(s) 38, 273, 398
MvaI CCWGG 2 cut(s) 38, 273
MwoI GCNNNNNNNGC 1 cut(s) 167
NciI CCSGG 1 cut(s) 398
NdeII GATC 2 cut(s) 302, 501
NlaIII CATG 3 cut(s) 52, 106, 112
NlaIV GGNNCC 2 cut(s) 396, 427
NmeAIII GCCGAG 1 cut(s) 290
NmuCI GTSAC 1 cut(s) 142
NspI RCATGY 1 cut(s) 106
PciI ACATGT 1 cut(s) 102
Pfl23II CGTACG 1 cut(s) 505
PinAI ACCGGT 1 cut(s) 131
PkrI GCNGC 1 cut(s) 359
Ple19I CGATCG 1 cut(s) 504
PleI GAGTC 1 cut(s) 504
PpsI GAGTC 1 cut(s) 504
PscI ACATGT 1 cut(s) 102
Psp6I CCWGG 2 cut(s) 36, 271
PspGI CCWGG 2 cut(s) 36, 271
PspLI CGTACG 1 cut(s) 505
PspN4I GGNNCC 2 cut(s) 396, 427
PspPI GGNCC 6 cut(s) 34, 134, 183, 236, 262, 394
PsuI RGATCY 1 cut(s) 302
PvuI CGATCG 1 cut(s) 504
RsaI GTAC 6 cut(s) 89, 113, 350, 427, 455, 507
RsaNI GTAC 6 cut(s) 88, 112, 349, 426, 454, 506
RseI CAYNNNNRTG 2 cut(s) 107, 521
SaqAI TTAA 1 cut(s) 8
SatI GCNGC 1 cut(s) 358
Sau3AI GATC 2 cut(s) 302, 501
Sau96I GGNCC 6 cut(s) 34, 134, 183, 236, 262, 394
SchI GAGTC 1 cut(s) 504
ScrFI CCNGG 3 cut(s) 38, 273, 398
SduI GDGCHC 1 cut(s) 271
SetI ASST 6 cut(s) 117, 180, 261, 277, 411, 473
SfaNI GCATC 1 cut(s) 428
SinI GGWCC 3 cut(s) 34, 134, 394
SmiMI CAYNNNNRTG 2 cut(s) 107, 521
Sse9I AATT 1 cut(s) 194
SsiI CCGC 1 cut(s) 161
SspMI CTAG 3 cut(s) 306, 374, 447
StyD4I CCNGG 3 cut(s) 36, 271, 396
TaaI ACNGT 4 cut(s) 30, 205, 383, 523
TaqI TCGA 1 cut(s) 500
TasI AATT 1 cut(s) 194
TatI WGTACW 2 cut(s) 87, 348
Tru1I TTAA 1 cut(s) 8
Tru9I TTAA 1 cut(s) 8
TscAI CASTG 2 cut(s) 300, 528
TseFI GTSAC 1 cut(s) 142
TseI GCWGC 1 cut(s) 357
Tsp45I GTSAC 1 cut(s) 142
TspDTI ATGAA 1 cut(s) 51
TspGWI ACGGA 1 cut(s) 315
TspRI CASTG 2 cut(s) 300, 528
VpaK11BI GGWCC 3 cut(s) 34, 134, 394
XceI RCATGY 1 cut(s) 106
XcmI CCANNNNNNNNNTGG 1 cut(s) 28
XspI CTAG 3 cut(s) 306, 374, 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.