MD09G1057500.v1.1

Lamin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008382
Physical Location & Seq
Reverse (-)
3807265 .. 3809156
1892 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1057500.v1.1.491

Sequence Viewer

Length: 522 bp
ATGGAGAAAGAGAGAGCTACGGCGGTGGTGGTGCTTTTGTGCTTGGTGGTGGTGATGGAACTTCCAGAAGCATCAGCTACGAGATATATGGTGGGAGGGAAAATGGGTTGGACCAGCAATGTCAACTATACTATTTGGGCTAAGGATCAGCACTTTTACAATGGAGACTGGCTCTTTTTTGTTTATGATAGGAACCAAATGGATGTTATAGAGGTGAACCAGACAAACTATGTCTCGTGCAATGCTGAACATCCCCTTCACAACTGGACCACCGGAGCCGGAAGAGATGTGGTTCCGCTGAATGTGACAAGGGACTACTACTTCATTACGAGCAAGGGGTACTGCTATGGTGGCATGAAGCTCGCCGTTAAAGTTGAAAACATGCCTCCACCCCCCGAAGCCGCCCCAGTGAAGAGCAAGAGCAGTGACCTAACACCTGCTTTCGGGTCCCAGTTTGTTTTGCCCTCAGTTCTCGCCATCGGCACAATGTGGGACGTTTTAGTTCGCTGTGAACGCATATGA

Protein Analysis

174

Amino Acids

19.48

Weight (kDa)

5.89

Isoelectric Point (pI)

31.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 37 - 119 9.5e-21 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 445
Acc36I ACCTGC 1 cut(s) 445
AciI CCGC 3 cut(s) 23, 296, 402
AclWI GGATC 1 cut(s) 153
AdeI CACNNNGTG 1 cut(s) 489
AfaI GTAC 1 cut(s) 341
AfiI CCNNNNNNNGG 1 cut(s) 443
AgsI TTSAA 1 cut(s) 377
AluBI AGCT 3 cut(s) 17, 77, 361
AluI AGCT 3 cut(s) 17, 77, 361
Alw26I GTCTC 2 cut(s) 159, 238
AlwI GGATC 1 cut(s) 153
AspS9I GGNCC 3 cut(s) 111, 267, 447
AsuHPI GGTGA 2 cut(s) 64, 226
AvaII GGWCC 3 cut(s) 111, 267, 447
BauI CACGAG 1 cut(s) 235
BccI CCATC 2 cut(s) 49, 485
BceAI ACGGC 2 cut(s) 36, 350
BcgI CGANNNNNNTGC 2 cut(s) 343, 377
BcoDI GTCTC 2 cut(s) 159, 238
BfuAI ACCTGC 1 cut(s) 445
BisI GCNGC 1 cut(s) 402
BlsI GCNGC 1 cut(s) 403
Bme18I GGWCC 3 cut(s) 111, 267, 447
BmgT120I GGNCC 3 cut(s) 111, 267, 447
BmiI GGNNCC 5 cut(s) 194, 277, 294, 448, 449
BmrI ACTGGG 2 cut(s) 401, 445
BmsI GCATC 1 cut(s) 80
BmuI ACTGGG 2 cut(s) 401, 445
BplI GAGNNNNNCTC 2 cut(s) 156, 188
Bpu10I CCTNAGC 1 cut(s) 141
BsaWI WCCGGW 1 cut(s) 272
Bsc4I CCNNNNNNNGG 1 cut(s) 443
Bse1I ACTGG 4 cut(s) 173, 269, 407, 451
Bse3DI GCAATG 2 cut(s) 124, 247
BseGI GGATG 2 cut(s) 208, 250
BseLI CCNNNNNNNGG 1 cut(s) 443
BseMI GCAATG 2 cut(s) 124, 247
BseMII CTCAG 1 cut(s) 480
BseNI ACTGG 4 cut(s) 173, 269, 407, 451
BsiSI CCGG 2 cut(s) 273, 279
BslFI GGGAC 3 cut(s) 326, 433, 506
BslI CCNNNNNNNGG 1 cut(s) 443
BsmAI GTCTC 2 cut(s) 159, 238
BsmFI GGGAC 3 cut(s) 326, 433, 506
Bsp143I GATC 1 cut(s) 145
BspACI CCGC 3 cut(s) 23, 296, 402
BspCNI CTCAG 1 cut(s) 479
BspLI GGNNCC 5 cut(s) 194, 277, 294, 448, 449
BspMI ACCTGC 1 cut(s) 445
BspPI GGATC 1 cut(s) 153
BspQI GCTCTTC 1 cut(s) 407
BsrDI GCAATG 2 cut(s) 124, 247
BsrI ACTGG 4 cut(s) 173, 269, 407, 451
BssMI GATC 1 cut(s) 145
BssSI CACGAG 1 cut(s) 235
Bst2BI CACGAG 1 cut(s) 235
Bst6I CTCTTC 2 cut(s) 277, 407
BstC8I GCNNGC 1 cut(s) 363
BstDEI CTNAG 2 cut(s) 141, 466
BstF5I GGATG 2 cut(s) 208, 250
BstKTI GATC 1 cut(s) 148
BstMAI GTCTC 2 cut(s) 159, 238
BstMBI GATC 1 cut(s) 145
BstMWI GCNNNNNNNGC 2 cut(s) 351, 513
BstNSI RCATGY 1 cut(s) 385
BtsCI GGATG 2 cut(s) 208, 250
BtsI GCAGTG 1 cut(s) 430
BtsIMutI CAGTG 2 cut(s) 414, 430
BveI ACCTGC 1 cut(s) 445
Cac8I GCNNGC 1 cut(s) 363
Cfr13I GGNCC 3 cut(s) 111, 267, 447
Csp6I GTAC 1 cut(s) 340
CviAII CATG 2 cut(s) 355, 382
CviJI RGCY 7 cut(s) 17, 77, 140, 172, 278, 361, 401
CviKI_1 RGCY 7 cut(s) 17, 77, 140, 172, 278, 361, 401
CviQI GTAC 1 cut(s) 340
DdeI CTNAG 2 cut(s) 141, 466
DpnI GATC 1 cut(s) 147
DpnII GATC 1 cut(s) 145
DraIII CACNNNGTG 1 cut(s) 489
Eam1104I CTCTTC 2 cut(s) 277, 407
EarI CTCTTC 2 cut(s) 277, 407
Eco47I GGWCC 3 cut(s) 111, 267, 447
EcoO109I RGGNCCY 1 cut(s) 447
FaeI CATG 2 cut(s) 358, 385
FaqI GGGAC 3 cut(s) 326, 433, 506
FatI CATG 2 cut(s) 354, 381
FauNDI CATATG 1 cut(s) 518
Fnu4HI GCNGC 1 cut(s) 402
FokI GGATG 2 cut(s) 215, 237
Fsp4HI GCNGC 1 cut(s) 402
GluI GCNGC 1 cut(s) 402
HapII CCGG 2 cut(s) 273, 279
Hin1II CATG 2 cut(s) 358, 385
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HpaII CCGG 2 cut(s) 273, 279
HphI GGTGA 2 cut(s) 64, 226
Hpy166II GTNNAC 3 cut(s) 124, 217, 512
Hpy188III TCNNGA 1 cut(s) 65
Hpy8I GTNNAC 3 cut(s) 124, 217, 512
HpyAV CCTTC 1 cut(s) 266
HpyCH4IV ACGT 1 cut(s) 495
HpyCH4V TGCA 1 cut(s) 240
HpyF10VI GCNNNNNNNGC 2 cut(s) 351, 513
HpyF3I CTNAG 2 cut(s) 141, 466
HpySE526I ACGT 1 cut(s) 495
Hsp92II CATG 2 cut(s) 358, 385
KflI GGGWCCC 1 cut(s) 447
Kzo9I GATC 1 cut(s) 145
LguI GCTCTTC 1 cut(s) 407
LmnI GCTCC 1 cut(s) 275
LweI GCATC 1 cut(s) 80
MaeII ACGT 1 cut(s) 495
MaeIII GTNAC 2 cut(s) 304, 425
MalI GATC 1 cut(s) 147
MboI GATC 1 cut(s) 145
MboII GAAGA 2 cut(s) 294, 424
MmeI TCCRAC 1 cut(s) 89
MnlI CCTC 4 cut(s) 89, 205, 396, 475
MseI TTAA 1 cut(s) 369
MspA1I CMGCKG 1 cut(s) 298
MspI CCGG 2 cut(s) 273, 279
MwoI GCNNNNNNNGC 2 cut(s) 351, 513
NdeI CATATG 1 cut(s) 518
NdeII GATC 1 cut(s) 145
NlaIII CATG 2 cut(s) 358, 385
NlaIV GGNNCC 5 cut(s) 194, 277, 294, 448, 449
NmuCI GTSAC 2 cut(s) 304, 425
NspI RCATGY 1 cut(s) 385
PaqCI CACCTGC 1 cut(s) 445
PciSI GCTCTTC 1 cut(s) 407
PkrI GCNGC 1 cut(s) 403
PpuMI RGGWCCY 1 cut(s) 447
Psp5II RGGWCCY 1 cut(s) 447
PspN4I GGNNCC 5 cut(s) 194, 277, 294, 448, 449
PspPI GGNCC 3 cut(s) 111, 267, 447
PspPPI RGGWCCY 1 cut(s) 447
RsaI GTAC 1 cut(s) 341
RsaNI GTAC 1 cut(s) 340
SapI GCTCTTC 1 cut(s) 407
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 1 cut(s) 402
Sau3AI GATC 1 cut(s) 145
Sau96I GGNCC 3 cut(s) 111, 267, 447
SetI ASST 7 cut(s) 19, 79, 216, 363, 432, 439, 498
SfaNI GCATC 1 cut(s) 80
SinI GGWCC 3 cut(s) 111, 267, 447
SsiI CCGC 3 cut(s) 23, 296, 402
TaiI ACGT 1 cut(s) 498
TauI GCSGC 1 cut(s) 404
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 2 cut(s) 414, 430
TseFI GTSAC 2 cut(s) 304, 425
Tsp45I GTSAC 2 cut(s) 304, 425
TspDTI ATGAA 2 cut(s) 313, 371
TspRI CASTG 2 cut(s) 414, 430
VpaK11BI GGWCC 3 cut(s) 111, 267, 447
XceI RCATGY 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.