RchiOBHm_Chr2g0166941

Lamin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008382
Physical Location & Seq
Forward (+)
81638399 .. 81640117
1719 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53476

Sequence Viewer

Length: 537 bp
ATGGAGAGGGTGAGAAGAGGGTTTGGGTCTGGTTCAGCTATGGCGGCTGTGGTGCTTTTGGCCTTGGTTTTTATCATGGTTCCAGAAGCCTCAGCTACTCGTTTTATTGTGGGAGGTAATAAGGGCTGGACTACCAATGTTAACTACACAATCTGGGCCCAGGGCATGCACTTCTACAATGGAGATTGGCTCTTTTTTGTGTACGATAGGAACCAGCAGAATATTCTAGAGGTGAACAAGACAGACTATGAGGCATGCAATTCAGATCATCCACTTCACAATTGGACTACAGGAGCTGGAAGAGATGTGGTTCCACTAAATGTGACCAGGCGCTACTATTTCTTGAGCGGCAAGGGCTTCTGCTACAGTGGCATGAAGTTGGCTATTCAGGTTGAAACCCCACCTCCACCTCCCAAGGCTGCTCCGGTGGTGAGCGAGGGCAGCGCTCCATTAGCGCCTGTTTTTAGATCCCAATTTGTGTTGCCAGCCATTTTTGCTATTGGTGCAGTTTGGGATGCATTTCTTAGGTTCTGCTAG

Protein Analysis

178

Amino Acids

19.76

Weight (kDa)

8.84

Isoelectric Point (pI)

44.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 43 - 125 4.3e-23 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 348
AciI CCGC 2 cut(s) 44, 348
AclWI GGATC 1 cut(s) 462
AfaI GTAC 1 cut(s) 203
AfeI AGCGCT 1 cut(s) 445
AgsI TTSAA 1 cut(s) 395
AjnI CCWGG 2 cut(s) 159, 326
AluBI AGCT 3 cut(s) 38, 95, 296
AluI AGCT 3 cut(s) 38, 95, 296
AlwI GGATC 1 cut(s) 462
AlwNI CAGNNNCTG 1 cut(s) 296
Aor51HI AGCGCT 1 cut(s) 445
AoxI GGCC 2 cut(s) 60, 156
ApaI GGGCCC 1 cut(s) 160
ApeKI GCWGC 2 cut(s) 419, 441
AspLEI GCGC 3 cut(s) 333, 446, 457
AspS9I GGNCC 2 cut(s) 156, 157
AsuHPI GGTGA 3 cut(s) 22, 244, 442
BaeGI GKGCMC 1 cut(s) 160
BanII GRGCYC 1 cut(s) 160
BbvCI CCTCAGC 1 cut(s) 91
BbvI GCAGC 2 cut(s) 406, 453
BciT130I CCWGG 2 cut(s) 161, 328
BfaI CTAG 2 cut(s) 227, 535
BfmI CTRYAG 2 cut(s) 288, 364
BfoI RGCGCY 3 cut(s) 334, 447, 458
BisI GCNGC 4 cut(s) 45, 349, 420, 442
BlsI GCNGC 4 cut(s) 46, 350, 421, 443
Bme1390I CCNGG 2 cut(s) 161, 328
BmgT120I GGNCC 2 cut(s) 156, 157
BmiI GGNNCC 4 cut(s) 81, 158, 212, 312
BmrFI CCNGG 2 cut(s) 161, 328
BmsI GCATC 1 cut(s) 505
BplI GAGNNNNNCTC 2 cut(s) 174, 206
Bpu10I CCTNAGC 1 cut(s) 91
BpuEI CTTGAG 1 cut(s) 364
BsaJI CCNNGG 4 cut(s) 63, 159, 160, 414
BsaWI WCCGGW 1 cut(s) 424
BsaXI ACNNNNNCTCC 2 cut(s) 388, 418
BseBI CCWGG 2 cut(s) 161, 328
BseDI CCNNGG 4 cut(s) 63, 159, 160, 414
BseGI GGATG 2 cut(s) 268, 520
BseMII CTCAG 1 cut(s) 105
BseSI GKGCMC 1 cut(s) 160
BseXI GCAGC 2 cut(s) 406, 453
BsgI GTGCAG 1 cut(s) 525
BshFI GGCC 2 cut(s) 62, 158
BsiSI CCGG 1 cut(s) 425
BsnI GGCC 2 cut(s) 62, 158
Bsp120I GGGCCC 1 cut(s) 156
Bsp1286I GDGCHC 1 cut(s) 160
Bsp143I GATC 2 cut(s) 265, 467
BspACI CCGC 2 cut(s) 44, 348
BspANI GGCC 2 cut(s) 62, 158
BspCNI CTCAG 1 cut(s) 104
BspLI GGNNCC 4 cut(s) 81, 158, 212, 312
BspPI GGATC 1 cut(s) 462
BsrBI CCGCTC 1 cut(s) 348
BssECI CCNNGG 4 cut(s) 63, 159, 160, 414
BssMI GATC 2 cut(s) 265, 467
BssT1I CCWWGG 2 cut(s) 63, 414
Bst2UI CCWGG 2 cut(s) 161, 328
Bst4CI ACNGT 1 cut(s) 368
Bst6I CTCTTC 2 cut(s) 10, 295
BstC8I GCNNGC 3 cut(s) 167, 256, 486
BstDEI CTNAG 2 cut(s) 91, 524
BstF5I GGATG 2 cut(s) 268, 520
BstH2I RGCGCY 3 cut(s) 334, 447, 458
BstHHI GCGC 3 cut(s) 333, 446, 457
BstKTI GATC 2 cut(s) 268, 470
BstMBI GATC 2 cut(s) 265, 467
BstMWI GCNNNNNNNGC 7 cut(s) 44, 354, 369, 441, 452, 494, 503
BstNI CCWGG 2 cut(s) 161, 328
BstNSI RCATGY 2 cut(s) 169, 258
BstSCI CCNGG 2 cut(s) 159, 326
BstSFI CTRYAG 2 cut(s) 288, 364
BstSLI GKGCMC 1 cut(s) 160
BstV1I GCAGC 2 cut(s) 406, 453
BstX2I RGATCY 1 cut(s) 467
BstYI RGATCY 1 cut(s) 467
BsuRI GGCC 2 cut(s) 62, 158
BtsCI GGATG 2 cut(s) 268, 520
BtsIMutI CAGTG 1 cut(s) 373
Cac8I GCNNGC 3 cut(s) 167, 256, 486
CaiI CAGNNNCTG 1 cut(s) 296
CfoI GCGC 3 cut(s) 333, 446, 457
Cfr13I GGNCC 2 cut(s) 156, 157
Csp6I GTAC 1 cut(s) 202
CviAII CATG 4 cut(s) 76, 166, 255, 373
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 2 cut(s) 91, 524
DpnI GATC 2 cut(s) 267, 469
DpnII GATC 2 cut(s) 265, 467
Eam1104I CTCTTC 2 cut(s) 10, 295
EarI CTCTTC 2 cut(s) 10, 295
Eco130I CCWWGG 2 cut(s) 63, 414
Eco24I GRGCYC 1 cut(s) 160
Eco47III AGCGCT 1 cut(s) 445
EcoRII CCWGG 2 cut(s) 159, 326
EcoT14I CCWWGG 2 cut(s) 63, 414
EcoT22I ATGCAT 1 cut(s) 520
EcoT38I GRGCYC 1 cut(s) 160
ErhI CCWWGG 2 cut(s) 63, 414
FaeI CATG 4 cut(s) 79, 169, 258, 376
FaiI YATR 6 cut(s) 41, 77, 167, 249, 256, 374
FatI CATG 4 cut(s) 75, 165, 254, 372
Fnu4HI GCNGC 4 cut(s) 45, 349, 420, 442
FokI GGATG 2 cut(s) 255, 527
FriOI GRGCYC 1 cut(s) 160
Fsp4HI GCNGC 4 cut(s) 45, 349, 420, 442
FspBI CTAG 2 cut(s) 227, 535
GlaI GCGC 3 cut(s) 332, 445, 456
GluI GCNGC 4 cut(s) 45, 349, 420, 442
HaeII RGCGCY 3 cut(s) 334, 447, 458
HaeIII GGCC 2 cut(s) 62, 158
HapII CCGG 1 cut(s) 425
HhaI GCGC 3 cut(s) 333, 446, 457
Hin1II CATG 4 cut(s) 79, 169, 258, 376
Hin6I GCGC 3 cut(s) 331, 444, 455
HinP1I GCGC 3 cut(s) 331, 444, 455
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HpaI GTTAAC 1 cut(s) 142
HpaII CCGG 1 cut(s) 425
HphI GGTGA 3 cut(s) 22, 244, 442
Hpy166II GTNNAC 3 cut(s) 142, 202, 235
Hpy188I TCNGA 1 cut(s) 265
Hpy188III TCNNGA 3 cut(s) 83, 227, 343
Hpy8I GTNNAC 3 cut(s) 142, 202, 235
HpyCH4III ACNGT 1 cut(s) 368
HpyCH4V TGCA 4 cut(s) 169, 258, 506, 518
HpyF10VI GCNNNNNNNGC 7 cut(s) 44, 354, 369, 441, 452, 494, 503
HpyF3I CTNAG 2 cut(s) 91, 524
Hsp92II CATG 4 cut(s) 79, 169, 258, 376
HspAI GCGC 3 cut(s) 331, 444, 455
KspAI GTTAAC 1 cut(s) 142
Kzo9I GATC 2 cut(s) 265, 467
LmnI GCTCC 3 cut(s) 293, 427, 451
Lsp1109I GCAGC 2 cut(s) 406, 453
LweI GCATC 1 cut(s) 505
MaeI CTAG 2 cut(s) 227, 535
MaeIII GTNAC 1 cut(s) 322
MalI GATC 2 cut(s) 267, 469
MbiI CCGCTC 1 cut(s) 348
MboI GATC 2 cut(s) 265, 467
MboII GAAGA 2 cut(s) 27, 312
MfeI CAATTG 1 cut(s) 280
MflI RGATCY 1 cut(s) 467
MhlI GDGCHC 1 cut(s) 160
MluCI AATT 3 cut(s) 259, 280, 473
MnlI CCTC 8 cut(s) 11, 100, 107, 223, 244, 414, 420, 430
Mph1103I ATGCAT 1 cut(s) 520
MseI TTAA 1 cut(s) 141
MspI CCGG 1 cut(s) 425
MspR9I CCNGG 2 cut(s) 161, 328
MunI CAATTG 1 cut(s) 280
MvaI CCWGG 2 cut(s) 161, 328
MwoI GCNNNNNNNGC 7 cut(s) 44, 354, 369, 441, 452, 494, 503
NdeII GATC 2 cut(s) 265, 467
NlaIII CATG 4 cut(s) 79, 169, 258, 376
NlaIV GGNNCC 4 cut(s) 81, 158, 212, 312
NmuCI GTSAC 1 cut(s) 322
NsiI ATGCAT 1 cut(s) 520
NspI RCATGY 2 cut(s) 169, 258
PaeI GCATGC 2 cut(s) 169, 258
PasI CCCWGGG 1 cut(s) 160
PkrI GCNGC 4 cut(s) 46, 350, 421, 443
Psp6I CCWGG 2 cut(s) 159, 326
PspGI CCWGG 2 cut(s) 159, 326
PspN4I GGNNCC 4 cut(s) 81, 158, 212, 312
PspOMI GGGCCC 1 cut(s) 156
PspPI GGNCC 2 cut(s) 156, 157
PstNI CAGNNNCTG 1 cut(s) 296
PsuI RGATCY 1 cut(s) 467
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
SaqAI TTAA 1 cut(s) 141
SatI GCNGC 4 cut(s) 45, 349, 420, 442
Sau3AI GATC 2 cut(s) 265, 467
Sau96I GGNCC 2 cut(s) 156, 157
ScrFI CCNGG 2 cut(s) 161, 328
SduI GDGCHC 1 cut(s) 160
SetI ASST 9 cut(s) 40, 97, 118, 234, 298, 393, 406, 412, 530
SfaNI GCATC 1 cut(s) 505
SfcI CTRYAG 2 cut(s) 288, 364
SmlI CTYRAG 1 cut(s) 343
SmoI CTYRAG 1 cut(s) 343
SphI GCATGC 2 cut(s) 169, 258
Sse9I AATT 3 cut(s) 259, 280, 473
SsiI CCGC 2 cut(s) 44, 348
SspI AATATT 1 cut(s) 223
SspMI CTAG 2 cut(s) 227, 535
StyD4I CCNGG 2 cut(s) 159, 326
StyI CCWWGG 2 cut(s) 63, 414
TaaI ACNGT 1 cut(s) 368
TasI AATT 3 cut(s) 259, 280, 473
TauI GCSGC 2 cut(s) 47, 351
Tru1I TTAA 1 cut(s) 141
Tru9I TTAA 1 cut(s) 141
TscAI CASTG 1 cut(s) 373
TseFI GTSAC 1 cut(s) 322
TseI GCWGC 2 cut(s) 419, 441
Tsp45I GTSAC 1 cut(s) 322
TspDTI ATGAA 1 cut(s) 389
TspRI CASTG 1 cut(s) 373
XbaI TCTAGA 1 cut(s) 226
XceI RCATGY 2 cut(s) 169, 258
XcmI CCANNNNNNNNNTGG 1 cut(s) 279
XspI CTAG 2 cut(s) 227, 535
Zsp2I ATGCAT 1 cut(s) 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.