AT5G16030

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006085
Physical Location & Seq
Forward (+)
5237970 .. 5240748
2779 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G16030.3

Sequence Viewer

Length: 1020 bp
ATGGCTTTGCGAGGAGATGAGCATGAGTTTATGAACTTAAGAGAATGGGATCGAAGAGCTCGATTAATCCGCGAAAACCCGAGTTCAAGAAGATTCTCAGCTTCATACATTGGAAGCTTCCGAGAAGATCACCACAAGTCTTCTTTTAGAACAACAAACTTCAACAACATCTCTAGTACTGCCTCTTCTCCTGGCTACACTCTCAAAGAAGAGATTGACCCATCAACATATTCCTTCACCAATGCACTCAAAGCATTACAAGCAAAGACAATGTACAATAACAGGGAATGGTTAGCACAAGAAGGGTTTGCATTGAATTCGAAGTGGAACGAAGCAGAGAAATATATCTGCAATCCATTGTCTGGAGAAGTACCAATGGAGTGTTTGTCTGCTAAAACATTAAGTGCAAGATCCTTCAGAAACTTATCAACCATGTCTGCTCCTCTTCACTTCCCTAGTCCCAATCCATTGATGAATAATATTGCTCAAAACAAACCAAATAATAATCCTAACGTTAGAGTCATTCACGAGGATCTTTATGCTCCTGATCCTGAACTTCTTGCTCTAGCGGAAAAGAAAGTTGTGGGGATGAAGCGAGATGTGGGCATACAGAGTACGACGTCGGTGGATCTTAGCTCCGGTAGCCCTAGTCCGGCAAAAACGCCTCCGATTATGGAAAGGTCGTTAAAACGACACGTGGAAGCTGATGATTGGCCAGTGGATATCAATCTCAAAGTGAAAGGTCAACAACAGGATGTGAAGTTGGAAGAGAAAGAAAAAGAAGAAGAGAAGCAAGATATGAGCAACGAAGAAGATGAAGAAGAAGAAGAAGAAGAGAAGCAAGATATGAGTGAAGAAGATGATAAAGAAGAAGAAGACGAACAAGAAGAAGAAGAGAAGACCAAGAAGAAGAAGAGAGGACCTGGATGTTTCTCATGGGTGAGATCAAGACAAAGACAAGCAAGAAAATCAAAGTATATCTTCCCTATTTGTGTTCCTCATCTTGTCAAAGGTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

339

Amino Acids

39.0

Weight (kDa)

5.45

Isoelectric Point (pI)

71.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 623
AccB7I CCANNNNNTGG 1 cut(s) 362
AccII CGCG 1 cut(s) 72
AciI CCGC 2 cut(s) 70, 569
AclI AACGTT 1 cut(s) 513
AclWI GGATC 5 cut(s) 57, 405, 540, 542, 636
AcoI YGGCCR 1 cut(s) 713
AcsI RAATTY 1 cut(s) 316
AcuI CTGAAG 1 cut(s) 400
AcvI CACGTG 1 cut(s) 697
AcyI GRCGYC 1 cut(s) 620
AfaI GTAC 4 cut(s) 178, 275, 372, 616
AfiI CCNNNNNNNGG 2 cut(s) 362, 652
AflII CTTAAG 1 cut(s) 37
AflIII ACRYGT 1 cut(s) 694
AgsI TTSAA 3 cut(s) 87, 163, 316
AjnI CCWGG 2 cut(s) 190, 922
AluBI AGCT 5 cut(s) 59, 101, 117, 636, 704
AluI AGCT 5 cut(s) 59, 101, 117, 636, 704
Alw21I GWGCWC 1 cut(s) 61
AlwI GGATC 5 cut(s) 57, 405, 540, 542, 636
Ama87I CYCGRG 1 cut(s) 79
AoxI GGCC 1 cut(s) 713
ApoI RAATTY 1 cut(s) 316
AseI ATTAAT 1 cut(s) 65
AspS9I GGNCC 1 cut(s) 920
AsuHPI GGTGA 3 cut(s) 122, 229, 952
AsuII TTCGAA 1 cut(s) 320
AvaI CYCGRG 1 cut(s) 79
AvaII GGWCC 1 cut(s) 920
BalI TGGCCA 1 cut(s) 715
BanII GRGCYC 1 cut(s) 61
BarI GAAGNNNNNNTAC 2 cut(s) 169, 201
BauI CACGAG 1 cut(s) 527
BbrPI CACGTG 1 cut(s) 697
BbsI GAAGAC 3 cut(s) 132, 882, 905
Bbv12I GWGCWC 1 cut(s) 61
BccI CCATC 1 cut(s) 229
BcgI CGANNNNNNTGC 2 cut(s) 300, 334
BciT130I CCWGG 2 cut(s) 192, 924
BfaI CTAG 4 cut(s) 174, 456, 566, 648
BfrI CTTAAG 1 cut(s) 37
BmcAI AGTACT 1 cut(s) 178
Bme1390I CCNGG 2 cut(s) 192, 924
Bme18I GGWCC 1 cut(s) 920
BmeT110I CYCGRG 1 cut(s) 79
BmgT120I GGNCC 1 cut(s) 920
BmrFI CCNGG 2 cut(s) 192, 924
BpiI GAAGAC 3 cut(s) 132, 882, 905
BpmI CTGGAG 1 cut(s) 384
Bpu14I TTCGAA 1 cut(s) 320
BsaAI YACGTR 1 cut(s) 697
BsaBI GATNNNNATC 1 cut(s) 726
BsaHI GRCGYC 1 cut(s) 620
BsaWI WCCGGW 1 cut(s) 638
BsaXI ACNNNNNCTCC 2 cut(s) 371, 401
Bsc4I CCNNNNNNNGG 2 cut(s) 362, 652
Bse1I ACTGG 1 cut(s) 716
Bse8I GATNNNNATC 1 cut(s) 726
BseBI CCWGG 2 cut(s) 192, 924
BseGI GGATG 3 cut(s) 594, 760, 932
BseJI GATNNNNATC 1 cut(s) 726
BseLI CCNNNNNNNGG 2 cut(s) 362, 652
BseMII CTCAG 1 cut(s) 111
BseNI ACTGG 1 cut(s) 716
BseRI GAGGAG 2 cut(s) 27, 432
Bsh1236I CGCG 1 cut(s) 72
BshFI GGCC 1 cut(s) 715
BsiHKAI GWGCWC 1 cut(s) 61
BsiHKCI CYCGRG 1 cut(s) 79
BsiSI CCGG 2 cut(s) 639, 653
BslFI GGGAC 1 cut(s) 444
BslI CCNNNNNNNGG 2 cut(s) 362, 652
BsmFI GGGAC 1 cut(s) 444
BsnI GGCC 1 cut(s) 715
BsoBI CYCGRG 1 cut(s) 79
Bsp119I TTCGAA 1 cut(s) 320
Bsp1286I GDGCHC 1 cut(s) 61
Bsp1407I TGTACA 1 cut(s) 273
Bsp143I GATC 7 cut(s) 49, 127, 410, 532, 547, 628, 944
BspACI CCGC 2 cut(s) 70, 569
BspANI GGCC 1 cut(s) 715
BspCNI CTCAG 1 cut(s) 110
BspFNI CGCG 1 cut(s) 72
BspPI GGATC 5 cut(s) 57, 405, 540, 542, 636
BspQI GCTCTTC 1 cut(s) 49
BspT104I TTCGAA 1 cut(s) 320
BspTI CTTAAG 1 cut(s) 37
BsrGI TGTACA 1 cut(s) 273
BsrI ACTGG 1 cut(s) 716
BssMI GATC 7 cut(s) 49, 127, 410, 532, 547, 628, 944
BssNI GRCGYC 1 cut(s) 620
BssSI CACGAG 1 cut(s) 527
Bst2BI CACGAG 1 cut(s) 527
Bst2UI CCWGG 2 cut(s) 192, 924
Bst6I CTCTTC 9 cut(s) 49, 190, 204, 450, 762, 780, 828, 888, 908
BstACI GRCGYC 1 cut(s) 620
BstAFI CTTAAG 1 cut(s) 37
BstAUI TGTACA 1 cut(s) 273
BstBAI YACGTR 1 cut(s) 697
BstBI TTCGAA 1 cut(s) 320
BstDEI CTNAG 2 cut(s) 97, 632
BstF5I GGATG 3 cut(s) 594, 760, 932
BstFNI CGCG 1 cut(s) 72
BstKTI GATC 7 cut(s) 52, 130, 413, 535, 550, 631, 947
BstMBI GATC 7 cut(s) 49, 127, 410, 532, 547, 628, 944
BstMWI GCNNNNNNNGC 3 cut(s) 251, 260, 642
BstNI CCWGG 2 cut(s) 192, 924
BstSCI CCNGG 2 cut(s) 190, 922
BstUI CGCG 1 cut(s) 72
BstV2I GAAGAC 3 cut(s) 132, 882, 905
BstX2I RGATCY 3 cut(s) 410, 532, 628
BstYI RGATCY 3 cut(s) 410, 532, 628
BsuRI GGCC 1 cut(s) 715
BtsCI GGATG 3 cut(s) 594, 760, 932
BtsIMutI CAGTG 1 cut(s) 723
Cfr13I GGNCC 1 cut(s) 920
Csp6I GTAC 4 cut(s) 177, 274, 371, 615
CviAII CATG 3 cut(s) 23, 433, 936
CviJI RGCY 9 cut(s) 5, 59, 101, 117, 195, 636, 645, 704, 715
CviKI_1 RGCY 9 cut(s) 5, 59, 101, 117, 195, 636, 645, 704, 715
CviQI GTAC 4 cut(s) 177, 274, 371, 615
DdeI CTNAG 2 cut(s) 97, 632
DpnI GATC 7 cut(s) 51, 129, 412, 534, 549, 630, 946
DpnII GATC 7 cut(s) 49, 127, 410, 532, 547, 628, 944
EaeI YGGCCR 1 cut(s) 713
Eam1104I CTCTTC 9 cut(s) 49, 190, 204, 450, 762, 780, 828, 888, 908
EarI CTCTTC 9 cut(s) 49, 190, 204, 450, 762, 780, 828, 888, 908
Ecl136II GAGCTC 1 cut(s) 59
Eco24I GRGCYC 1 cut(s) 61
Eco32I GATATC 1 cut(s) 724
Eco47I GGWCC 1 cut(s) 920
Eco53kI GAGCTC 1 cut(s) 59
Eco57I CTGAAG 1 cut(s) 400
Eco72I CACGTG 1 cut(s) 697
Eco88I CYCGRG 1 cut(s) 79
EcoICRI GAGCTC 1 cut(s) 59
EcoO109I RGGNCCY 1 cut(s) 920
EcoRI GAATTC 1 cut(s) 316
EcoRII CCWGG 2 cut(s) 190, 922
EcoRV GATATC 1 cut(s) 724
EcoT38I GRGCYC 1 cut(s) 61
FaeI CATG 3 cut(s) 26, 436, 939
FalI AAGNNNNNCTT 2 cut(s) 965, 997
FaqI GGGAC 1 cut(s) 444
FatI CATG 3 cut(s) 22, 432, 935
FokI GGATG 3 cut(s) 601, 767, 939
FriOI GRGCYC 1 cut(s) 61
FspBI CTAG 4 cut(s) 174, 456, 566, 648
GsuI CTGGAG 1 cut(s) 384
HaeIII GGCC 1 cut(s) 715
HapII CCGG 2 cut(s) 639, 653
Hin1I GRCGYC 1 cut(s) 620
Hin1II CATG 3 cut(s) 26, 436, 939
HincII GTYRAC 1 cut(s) 746
HindII GTYRAC 1 cut(s) 746
HindIII AAGCTT 1 cut(s) 115
HinfI GANTC 2 cut(s) 93, 519
HpaII CCGG 2 cut(s) 639, 653
HphI GGTGA 3 cut(s) 122, 229, 952
Hpy166II GTNNAC 1 cut(s) 746
Hpy188I TCNGA 3 cut(s) 122, 419, 669
Hpy188III TCNNGA 6 cut(s) 87, 363, 527, 545, 551, 948
Hpy8I GTNNAC 1 cut(s) 746
Hpy99I CGWCG 2 cut(s) 622, 625
HpyAV CCTTC 3 cut(s) 244, 296, 424
HpyCH4IV ACGT 3 cut(s) 513, 620, 696
HpyCH4V TGCA 4 cut(s) 245, 311, 351, 407
HpyF10VI GCNNNNNNNGC 3 cut(s) 251, 260, 642
HpyF3I CTNAG 2 cut(s) 97, 632
HpySE526I ACGT 3 cut(s) 513, 620, 696
Hsp92I GRCGYC 1 cut(s) 620
Hsp92II CATG 3 cut(s) 26, 436, 939
Kzo9I GATC 7 cut(s) 49, 127, 410, 532, 547, 628, 944
LguI GCTCTTC 1 cut(s) 49
LmnI GCTCC 3 cut(s) 445, 547, 641
MaeI CTAG 4 cut(s) 174, 456, 566, 648
MaeII ACGT 3 cut(s) 513, 620, 696
MalI GATC 7 cut(s) 51, 129, 412, 534, 549, 630, 946
MboI GATC 7 cut(s) 49, 127, 410, 532, 547, 628, 944
MflI RGATCY 3 cut(s) 410, 532, 628
MhlI GDGCHC 1 cut(s) 61
MlsI TGGCCA 1 cut(s) 715
MluCI AATT 1 cut(s) 316
MluNI TGGCCA 1 cut(s) 715
MlyI GAGTC 1 cut(s) 528
MmeI TCCRAC 1 cut(s) 744
MnlI CCTC 7 cut(s) 5, 193, 453, 523, 675, 911, 1008
Mox20I TGGCCA 1 cut(s) 715
MscI TGGCCA 1 cut(s) 715
MseI TTAA 4 cut(s) 38, 65, 401, 686
Msp20I TGGCCA 1 cut(s) 715
MspCI CTTAAG 1 cut(s) 37
MspI CCGG 2 cut(s) 639, 653
MspR9I CCNGG 2 cut(s) 192, 924
MvaI CCWGG 2 cut(s) 192, 924
MvnI CGCG 1 cut(s) 72
MwoI GCNNNNNNNGC 3 cut(s) 251, 260, 642
NdeII GATC 7 cut(s) 49, 127, 410, 532, 547, 628, 944
NlaIII CATG 3 cut(s) 26, 436, 939
NspV TTCGAA 1 cut(s) 320
PciSI GCTCTTC 1 cut(s) 49
PcsI WCGNNNNNNNCGW 1 cut(s) 58
PfeI GAWTC 1 cut(s) 93
PflMI CCANNNNNTGG 1 cut(s) 362
PleI GAGTC 1 cut(s) 527
PmaCI CACGTG 1 cut(s) 697
PmlI CACGTG 1 cut(s) 697
PpsI GAGTC 1 cut(s) 527
Ppu21I YACGTR 1 cut(s) 697
PpuMI RGGWCCY 1 cut(s) 920
PshBI ATTAAT 1 cut(s) 65
Psp124BI GAGCTC 1 cut(s) 61
Psp1406I AACGTT 1 cut(s) 513
Psp5II RGGWCCY 1 cut(s) 920
Psp6I CCWGG 2 cut(s) 190, 922
PspCI CACGTG 1 cut(s) 697
PspGI CCWGG 2 cut(s) 190, 922
PspPI GGNCC 1 cut(s) 920
PspPPI RGGWCCY 1 cut(s) 920
PsuI RGATCY 3 cut(s) 410, 532, 628
RsaI GTAC 4 cut(s) 178, 275, 372, 616
RsaNI GTAC 4 cut(s) 177, 274, 371, 615
SacI GAGCTC 1 cut(s) 61
SapI GCTCTTC 1 cut(s) 49
SaqAI TTAA 4 cut(s) 38, 65, 401, 686
Sau3AI GATC 7 cut(s) 49, 127, 410, 532, 547, 628, 944
Sau96I GGNCC 1 cut(s) 920
ScaI AGTACT 1 cut(s) 178
SchI GAGTC 1 cut(s) 528
ScrFI CCNGG 2 cut(s) 192, 924
SduI GDGCHC 1 cut(s) 61
SfuI TTCGAA 1 cut(s) 320
SinI GGWCC 1 cut(s) 920
SmlI CTYRAG 1 cut(s) 37
SmoI CTYRAG 1 cut(s) 37
Sse9I AATT 1 cut(s) 316
SsiI CCGC 2 cut(s) 70, 569
SspI AATATT 1 cut(s) 481
SspMI CTAG 4 cut(s) 174, 456, 566, 648
SstI GAGCTC 1 cut(s) 61
StyD4I CCNGG 2 cut(s) 190, 922
TaiI ACGT 3 cut(s) 516, 623, 699
TaqI TCGA 3 cut(s) 52, 61, 320
TasI AATT 1 cut(s) 316
TatI WGTACW 2 cut(s) 176, 273
TfiI GAWTC 1 cut(s) 93
Tru1I TTAA 4 cut(s) 38, 65, 401, 686
Tru9I TTAA 4 cut(s) 38, 65, 401, 686
TscAI CASTG 1 cut(s) 723
TspDTI ATGAA 5 cut(s) 47, 93, 488, 605, 831
TspRI CASTG 1 cut(s) 723
Van91I CCANNNNNTGG 1 cut(s) 362
Vha464I CTTAAG 1 cut(s) 37
VpaK11BI GGWCC 1 cut(s) 920
VspI ATTAAT 1 cut(s) 65
XapI RAATTY 1 cut(s) 316
XspI CTAG 4 cut(s) 174, 456, 566, 648
ZraI GACGTC 1 cut(s) 621
ZrmI AGTACT 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.