AT5G18440

Nuclear fragile X mental retardation-interacting protein 1, conserved region (InterPro IPR019496)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005893
Physical Location & Seq
Forward (+)
6112738 .. 6115932
3195 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G18440.1

Sequence Viewer

Length: 1413 bp
ATGCGACCTCCGTACAATTCCTTCCAGCCTCAAGCTCGTCCTTATCGACCGCAACATCAACTACAACAACAACAACAGATCAATGGGTTTTCTAATCATCAACAACAACAACATAATGGGTATCAGAACCCAATGAACGCTAATCAATTAGGGATGATGAATCCACAGATGATGAATAATCCAATGATGGGTCACAACATGCCAATGCCCAATATGCCAATACATCCTCAGTTCTTTAACAATATGCCTCAACAACTTCCTCAGTTTGCTATGCCCAACCATATCAACCAGCTACTTCCCAATCTCTTGGGGAATCTTCAGTTTGCAGTTGCTAACAGTAACCTTATGGGTCACTCTCTTCCCAACTTTTTCCAGCCTTCTCTTGAACCTCATGCTTTCTCTTCTCGACCACAGCTTAATTCCTTTAACTCCCTTCCTTATCCTCCGGTTCCAAATCCCCATCAAAATCACCAATCAGGGCCTCCGGGTTTCTCAGAGCCAAGACCACAGGGGCAGTCTGTCGATAATACCAATGGTTCAGGTCCTAACGGGAATGATTTTCGAAATAAGTTTCCAAAGCATCAAAATTTCAAAGGCCCTGGTCAAGGATTCCAGAGACCTCAGTTGCATCAAGCAGATAATGGCAAGAGAAAGTCTGGATTCAATAAGGATCACAGGGGCAAAGGGAACAATAATAAGATGAAAACAGGGCTCGATGGATCTGATACTGGTAACATAGCTAAGGAGAAGAAAAGATCATATGCTTTGATGTATACACCAAGAGAAGTTCAGCAATGGCGTGAAGCTCGACGTAAGAATTATCCAACGAAATTCCTTGTTGAAAAGAAAGTAAAGAAAAATGTTTCAGCCAGCATCCTTGATGAGGAAGCAAAAATGCGTCGTCAGCAACTCCGGGAAGTTTTAGCAAAGCAGGCTGAGTTAGGTGTTGAAGTTGCAGAAGTTCCTTCGCATTATTTATCCAATAATGATGAACAAGTTAATGGAGATAGAGGAAACAACAATGGGCGAAAGGGACGATTCCAGAATAATAGACGTAATAAGAGAAGACATGACAGAAAAGATAAGTTTGATAATAAGAAACCAAGATTGGAAGACAAAAAATCGTCTCAAGATTCTTCCATAACGACTAGAAAACCTACATTGCTTGAGAAGCTCCTAAGCGCAGACATAAAGAGAGACAAAAGCCAGTTATTACAGGTCTTCCGCTTCATGGTAATGAATTCATTGTTGAAGGAGTTTCCAGAGCAACCTCTGAAGTTACCTTTGATTACAGTAAAAGAAACTGGCTGTGAAGATGCTATGGAAGATCCCTCAATTGAAGGTTTATGTGATGGCCTTAGTGATGGCGATGATGTGGATGGCGATGATAGCTCATGTGATGAAGATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko05164 map03015 map05164
Pfam Domains
Protein Families
NUFIP1

Protein Analysis

470

Amino Acids

53.64

Weight (kDa)

9.56

Isoelectric Point (pI)

55.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUFIP1 PF10453 242 - 291 5.6e-14 FMR1-interacting protein 1 (NUFIP1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 773
AciI CCGC 2 cut(s) 50, 1225
AclWI GGATC 3 cut(s) 678, 727, 1322
AcsI RAATTY 3 cut(s) 586, 830, 1240
AcuI CTGAAG 2 cut(s) 302, 1295
AfaI GTAC 1 cut(s) 14
AfiI CCNNNNNNNGG 4 cut(s) 188, 605, 883, 1231
AgsI TTSAA 7 cut(s) 386, 592, 664, 842, 950, 1252, 1340
AjnI CCWGG 1 cut(s) 598
AjuI GAANNNNNNNTTGG 2 cut(s) 1091, 1123
AluBI AGCT 7 cut(s) 35, 292, 415, 740, 806, 1174, 1392
AluI AGCT 7 cut(s) 35, 292, 415, 740, 806, 1174, 1392
Alw26I GTCTC 3 cut(s) 610, 1131, 1191
AlwI GGATC 3 cut(s) 678, 727, 1322
AoxI GGCC 3 cut(s) 479, 595, 1354
ApoI RAATTY 3 cut(s) 586, 830, 1240
AspLEI GCGC 1 cut(s) 1184
AspS9I GGNCC 3 cut(s) 479, 542, 596
AsuC2I CCSGG 2 cut(s) 486, 914
AsuHPI GGTGA 1 cut(s) 461
AsuII TTCGAA 1 cut(s) 562
AvaII GGWCC 1 cut(s) 542
BanII GRGCYC 1 cut(s) 714
BarI GAAGNNNNNNTAC 1 cut(s) 37
BbsI GAAGAC 3 cut(s) 1072, 1119, 1213
BccI CCATC 6 cut(s) 181, 468, 710, 1346, 1358, 1373
BciT130I CCWGG 1 cut(s) 600
BcnI CCSGG 2 cut(s) 486, 914
BcoDI GTCTC 3 cut(s) 610, 1131, 1191
BfaI CTAG 1 cut(s) 1149
Bme1390I CCNGG 3 cut(s) 486, 600, 914
Bme18I GGWCC 1 cut(s) 542
BmgT120I GGNCC 3 cut(s) 479, 542, 596
BmiI GGNNCC 1 cut(s) 450
BmrFI CCNGG 3 cut(s) 486, 600, 914
BmsI GCATC 4 cut(s) 589, 637, 882, 1306
BpiI GAAGAC 3 cut(s) 1072, 1119, 1213
Bpu10I CCTNAGC 2 cut(s) 741, 1178
Bpu14I TTCGAA 1 cut(s) 562
BpuEI CTTGAG 3 cut(s) 15, 1113, 1187
BpuMI CCSGG 2 cut(s) 486, 914
BsaI GGTCTC 1 cut(s) 610
BsaJI CCNNGG 1 cut(s) 598
BsaWI WCCGGW 1 cut(s) 445
Bsc4I CCNNNNNNNGG 4 cut(s) 188, 605, 883, 1231
Bse1I ACTGG 3 cut(s) 733, 1207, 1309
Bse3DI GCAATG 2 cut(s) 800, 1160
BseBI CCWGG 1 cut(s) 600
BseDI CCNNGG 1 cut(s) 598
BseGI GGATG 4 cut(s) 159, 223, 873, 1384
BseLI CCNNNNNNNGG 4 cut(s) 188, 605, 883, 1231
BseMI GCAATG 2 cut(s) 800, 1160
BseMII CTCAG 5 cut(s) 242, 275, 507, 635, 927
BseNI ACTGG 3 cut(s) 733, 1207, 1309
Bsh1285I CGRYCG 1 cut(s) 50
BshFI GGCC 3 cut(s) 481, 597, 1356
BsiEI CGRYCG 1 cut(s) 50
BsiSI CCGG 3 cut(s) 446, 485, 913
BslFI GGGAC 1 cut(s) 1047
BslI CCNNNNNNNGG 4 cut(s) 188, 605, 883, 1231
BsmAI GTCTC 3 cut(s) 610, 1131, 1191
BsmBI CGTCTC 1 cut(s) 1131
BsmFI GGGAC 1 cut(s) 1047
BsnI GGCC 3 cut(s) 481, 597, 1356
Bso31I GGTCTC 1 cut(s) 610
Bsp119I TTCGAA 1 cut(s) 562
Bsp1286I GDGCHC 1 cut(s) 714
Bsp143I GATC 5 cut(s) 78, 670, 719, 755, 1327
BspACI CCGC 2 cut(s) 50, 1225
BspANI GGCC 3 cut(s) 481, 597, 1356
BspCNI CTCAG 5 cut(s) 241, 274, 506, 634, 928
BspLI GGNNCC 1 cut(s) 450
BspPI GGATC 3 cut(s) 678, 727, 1322
BspT104I TTCGAA 1 cut(s) 562
BspTNI GGTCTC 1 cut(s) 610
BsrDI GCAATG 2 cut(s) 800, 1160
BsrI ACTGG 3 cut(s) 733, 1207, 1309
BssECI CCNNGG 1 cut(s) 598
BssMI GATC 5 cut(s) 78, 670, 719, 755, 1327
BssNAI GTATAC 1 cut(s) 774
Bst1107I GTATAC 1 cut(s) 774
Bst2UI CCWGG 1 cut(s) 600
Bst4CI ACNGT 2 cut(s) 338, 1294
Bst6I CTCTTC 2 cut(s) 363, 406
BstBI TTCGAA 1 cut(s) 562
BstC8I GCNNGC 2 cut(s) 871, 933
BstDEI CTNAG 8 cut(s) 228, 261, 493, 621, 741, 936, 1178, 1358
BstENI CCTNNNNNAGG 2 cut(s) 603, 881
BstF5I GGATG 4 cut(s) 159, 223, 873, 1384
BstHHI GCGC 1 cut(s) 1184
BstKTI GATC 5 cut(s) 81, 673, 722, 758, 1330
BstMAI GTCTC 3 cut(s) 610, 1131, 1191
BstMBI GATC 5 cut(s) 78, 670, 719, 755, 1327
BstMCI CGRYCG 1 cut(s) 50
BstMWI GCNNNNNNNGC 5 cut(s) 214, 904, 932, 1171, 1389
BstNI CCWGG 1 cut(s) 600
BstNSI RCATGY 1 cut(s) 202
BstSCI CCNGG 3 cut(s) 484, 598, 912
BstV2I GAAGAC 3 cut(s) 1072, 1119, 1213
BstX2I RGATCY 2 cut(s) 719, 1327
BstXI CCANNNNNNTGG 1 cut(s) 307
BstYI RGATCY 2 cut(s) 719, 1327
BstZ17I GTATAC 1 cut(s) 774
BsuRI GGCC 3 cut(s) 481, 597, 1356
BtgZI GCGATG 2 cut(s) 1383, 1398
BtsCI GGATG 4 cut(s) 159, 223, 873, 1384
Cac8I GCNNGC 2 cut(s) 871, 933
CfoI GCGC 1 cut(s) 1184
Cfr13I GGNCC 3 cut(s) 479, 542, 596
CseI GACGC 1 cut(s) 887
Csp6I GTAC 1 cut(s) 13
CviAII CATG 5 cut(s) 199, 392, 1070, 1231, 1395
CviQI GTAC 1 cut(s) 13
DdeI CTNAG 8 cut(s) 228, 261, 493, 621, 741, 936, 1178, 1358
DpnI GATC 5 cut(s) 80, 672, 721, 757, 1329
DpnII GATC 5 cut(s) 78, 670, 719, 755, 1327
Eam1104I CTCTTC 2 cut(s) 363, 406
EarI CTCTTC 2 cut(s) 363, 406
Eco24I GRGCYC 1 cut(s) 714
Eco31I GGTCTC 1 cut(s) 610
Eco47I GGWCC 1 cut(s) 542
Eco57I CTGAAG 2 cut(s) 302, 1295
EcoNI CCTNNNNNAGG 2 cut(s) 603, 881
EcoO109I RGGNCCY 3 cut(s) 479, 542, 596
EcoRI GAATTC 1 cut(s) 1240
EcoRII CCWGG 1 cut(s) 598
EcoT38I GRGCYC 1 cut(s) 714
Esp3I CGTCTC 1 cut(s) 1131
FaeI CATG 5 cut(s) 202, 395, 1073, 1234, 1398
FaqI GGGAC 1 cut(s) 1047
FatI CATG 5 cut(s) 198, 391, 1069, 1230, 1394
FauNDI CATATG 1 cut(s) 760
FblI GTMKAC 1 cut(s) 773
FokI GGATG 4 cut(s) 166, 210, 860, 1391
FriOI GRGCYC 1 cut(s) 714
FspBI CTAG 1 cut(s) 1149
GlaI GCGC 1 cut(s) 1183
HaeIII GGCC 3 cut(s) 481, 597, 1356
HapII CCGG 3 cut(s) 446, 485, 913
HgaI GACGC 1 cut(s) 887
HhaI GCGC 1 cut(s) 1184
Hin1II CATG 5 cut(s) 202, 395, 1073, 1234, 1398
Hin6I GCGC 1 cut(s) 1182
HinP1I GCGC 1 cut(s) 1182
HinfI GANTC 6 cut(s) 160, 313, 609, 660, 1038, 1133
HpaII CCGG 3 cut(s) 446, 485, 913
HphI GGTGA 1 cut(s) 461
Hpy166II GTNNAC 1 cut(s) 774
Hpy188I TCNGA 4 cut(s) 126, 496, 724, 1275
Hpy188III TCNNGA 7 cut(s) 383, 405, 613, 657, 1042, 1130, 1262
Hpy8I GTNNAC 1 cut(s) 774
Hpy99I CGWCG 2 cut(s) 813, 903
HpyAV CCTTC 6 cut(s) 31, 387, 443, 975, 1246, 1334
HpyCH4III ACNGT 2 cut(s) 338, 1294
HpyCH4IV ACGT 2 cut(s) 811, 1054
HpyCH4V TGCA 3 cut(s) 326, 628, 956
HpyF10VI GCNNNNNNNGC 5 cut(s) 214, 904, 932, 1171, 1389
HpyF3I CTNAG 8 cut(s) 228, 261, 493, 621, 741, 936, 1178, 1358
HpySE526I ACGT 2 cut(s) 811, 1054
Hsp92II CATG 5 cut(s) 202, 395, 1073, 1234, 1398
HspAI GCGC 1 cut(s) 1182
Kzo9I GATC 5 cut(s) 78, 670, 719, 755, 1327
LmnI GCTCC 1 cut(s) 1179
LweI GCATC 4 cut(s) 589, 637, 882, 1306
MaeI CTAG 1 cut(s) 1149
MaeII ACGT 2 cut(s) 811, 1054
MaeIII GTNAC 5 cut(s) 191, 338, 350, 731, 1278
MalI GATC 5 cut(s) 80, 672, 721, 757, 1329
MboI GATC 5 cut(s) 78, 670, 719, 755, 1327
MfeI CAATTG 1 cut(s) 1335
MflI RGATCY 2 cut(s) 719, 1327
MhlI GDGCHC 1 cut(s) 714
MluCI AATT 8 cut(s) 16, 146, 418, 586, 817, 830, 1240, 1335
MmeI TCCRAC 1 cut(s) 848
MseI TTAA 4 cut(s) 237, 417, 426, 999
MslI CAYNNNNRTG 2 cut(s) 203, 1235
MspI CCGG 3 cut(s) 446, 485, 913
MspR9I CCNGG 3 cut(s) 486, 600, 914
MunI CAATTG 1 cut(s) 1335
MvaI CCWGG 1 cut(s) 600
MwoI GCNNNNNNNGC 5 cut(s) 214, 904, 932, 1171, 1389
NciI CCSGG 2 cut(s) 486, 914
NdeI CATATG 1 cut(s) 760
NdeII GATC 5 cut(s) 78, 670, 719, 755, 1327
NlaIII CATG 5 cut(s) 202, 395, 1073, 1234, 1398
NlaIV GGNNCC 1 cut(s) 450
NmuCI GTSAC 2 cut(s) 191, 350
NspI RCATGY 1 cut(s) 202
NspV TTCGAA 1 cut(s) 562
PcsI WCGNNNNNNNCGW 1 cut(s) 43
PfeI GAWTC 6 cut(s) 160, 313, 609, 660, 1038, 1133
PfoI TCCNGGA 1 cut(s) 912
PpuMI RGGWCCY 1 cut(s) 542
Psp5II RGGWCCY 1 cut(s) 542
Psp6I CCWGG 1 cut(s) 598
PspGI CCWGG 1 cut(s) 598
PspN4I GGNNCC 1 cut(s) 450
PspPI GGNCC 3 cut(s) 479, 542, 596
PspPPI RGGWCCY 1 cut(s) 542
PsuI RGATCY 2 cut(s) 719, 1327
RsaI GTAC 1 cut(s) 14
RsaNI GTAC 1 cut(s) 13
RseI CAYNNNNRTG 2 cut(s) 203, 1235
SaqAI TTAA 4 cut(s) 237, 417, 426, 999
Sau3AI GATC 5 cut(s) 78, 670, 719, 755, 1327
Sau96I GGNCC 3 cut(s) 479, 542, 596
ScrFI CCNGG 3 cut(s) 486, 600, 914
SduI GDGCHC 1 cut(s) 714
SfaNI GCATC 4 cut(s) 589, 637, 882, 1306
SfuI TTCGAA 1 cut(s) 562
SinI GGWCC 1 cut(s) 542
SmiMI CAYNNNNRTG 2 cut(s) 203, 1235
SmlI CTYRAG 3 cut(s) 30, 1128, 1166
SmoI CTYRAG 3 cut(s) 30, 1128, 1166
Sse9I AATT 8 cut(s) 16, 146, 418, 586, 817, 830, 1240, 1335
SsiI CCGC 2 cut(s) 50, 1225
SspMI CTAG 1 cut(s) 1149
StyD4I CCNGG 3 cut(s) 484, 598, 912
TaaI ACNGT 2 cut(s) 338, 1294
TaiI ACGT 2 cut(s) 814, 1057
TaqI TCGA 6 cut(s) 46, 406, 522, 562, 714, 808
TasI AATT 8 cut(s) 16, 146, 418, 586, 817, 830, 1240, 1335
TfiI GAWTC 6 cut(s) 160, 313, 609, 660, 1038, 1133
Tru1I TTAA 4 cut(s) 237, 417, 426, 999
Tru9I TTAA 4 cut(s) 237, 417, 426, 999
TseFI GTSAC 2 cut(s) 191, 350
Tsp45I GTSAC 2 cut(s) 191, 350
TspDTI ATGAA 8 cut(s) 149, 173, 188, 716, 1005, 1219, 1233, 1253
VpaK11BI GGWCC 1 cut(s) 542
XagI CCTNNNNNAGG 2 cut(s) 603, 881
XapI RAATTY 3 cut(s) 586, 830, 1240
XceI RCATGY 1 cut(s) 202
XmiI GTMKAC 1 cut(s) 773
XspI CTAG 1 cut(s) 1149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.