Rw3G026000

Nuclear fragile X mental retardation-interacting protein 1 (NUFIP1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0005893
Physical Location & Seq
Forward (+)
35224355 .. 35231563
7209 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G026000.1

Sequence Viewer

Length: 1695 bp
ATGTTACCTCACTATAATTCCTTTCACCAAAATTCCGGCCAAGCTCACCCGGCCGGCGGTAATGGTGGTAGCTCTGCTCCATCTCCTCAACAGCAGCAGGGTATGCCATTTAATCCATCACTTGCAAATTCATGTAATATGTTGCCACCTTCGATGGGACCTCAGCCCGGTTTCATGAATTTATCTAACAATCTTCTTCCGATGCAAAATAACCATATGCGTGTGCCCATGTCGCAATTTGGTTCCCTTGGTCCCATATCTCAGCCAGGTTATCCCAGTGTTGGATTTTGTGGTCCACAGAATAATGCTCTCAATATGAACTTGGCTGCTTCCTATCCATTTAACGGGCAGTTATGCAATATGGGGCAGAATCTCAATCAAGTAAATCAAAATATGGGTTTTCTACCTGGTCAATTGTTTGGACAGAACCTGTTGAATTTACAGCTGATGAATCAAAATATTGGGCCATATGGGCAATTCTGTTCGCCGAATCTGTTGAATAGGAATCAGGTTGTGCCAGTGCAAATGCAAATGCAAAACGCTTGCCAAGGTGGTCCTAATTATGCATTTGGGGGGTCAAATCAAGCACCTCAGGCTGCAGTTCTGCAAAATCCTGCTTTTTTTGCAACTCCCAATTCTGGTAATGTGCACTCTCCAAATCAGGCCGGACAACAAATCAACCAGAATCAGCAAAACAACGTCCTCCGAGGGACACAGGGAAATCATATAAAAGCTGGTGGCGTTTACAATTCGAATTCTGACAGAAAAACTTCGCCAAGCAAAAATTTTATAAAGCATCCAAAACGAGGGTTGCAACCGCAAGGGGGATTTCAGAATTCTCAGTTTCATCACATCAAAAATGCAAAGGGAAAGTTTGCTTTTCCCAACAGGCAAAACAGAAAAGGGCTAAGCAATGAGAAGGAAGGAAAATCTGCTCTTCCCGACTCTATGAATCAAGGCAGGGAAAGGAAAAGATCTCTTTCTTTGTCCTATACGGAACAAGAAGTTCAACTGTGGCGTGAAGGACGTAGGAAGAACTACCCATCAAACACCAACATAGAGAAGAAGCTAAGTGAAAAGATGATAGACTCAGAGGCCATTGAGAGAGAGGCCAAAGCACGAAGGGAGCAACTCAAGGAGATTCTTACAAAGCAGGCTGAGATGGGAGTTGAAGTCGCTGAGATACCATCTCACTACCTCTCAGATTCAGCAAAACAAGGGAATGGAAGAGAGGAGAATAAGTCATGGACTAACAATCGGAGATCACAAAACAAGTTCGGTAAGAGAGACAGATATGATAAGAAGGACCGGTTTGCAAAGAGGCAAAGATCAGGTCAGAAGGATTCATCTAATGACCCTTCATTCAGCAAAAGGGAGCCAACCTTACTGCAGAAACTTCTTAGTTCAGATATCAAGAGAGATAAAAGTCACTTATGCCAGGTGTTTAGGTTCATGGTGACAAACTCTTTCTTTAAAGATTTTCCTGACAAACCTTTGAAATTTCCTACTTTAGTAGTTAAAGAAAGTGTGGTGGAGGAAGGTGTGGTTGAAGAGTCTTCAGTTGCCGGGAAAGGTGCTTATGGGGGCCGTGACAAGTCCATGTTTGAAAAAGTTGGTAACAATGATTATAAAGATTGTTGTCACAATGATGATTGTGTTGAAGATGAGGAAGAAGAGGGAGAGATCATCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko05164 map03015 map05164
Pfam Domains
Protein Families
NUFIP1

Protein Analysis

564

Amino Acids

62.83

Weight (kDa)

9.37

Isoelectric Point (pI)

53.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUFIP1 PF10453 328 - 360 1.1e-08 FMR1-interacting protein 1 (NUFIP1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 791, 1629
AciI CCGC 2 cut(s) 57, 818
AcoI YGGCCR 2 cut(s) 37, 51
AcsI RAATTY 8 cut(s) 31, 127, 178, 436, 754, 784, 835, 1499
AcuI CTGAAG 1 cut(s) 1542
AfiI CCNNNNNNNGG 8 cut(s) 56, 155, 167, 281, 344, 638, 806, 824
AgeI ACCGGT 1 cut(s) 1308
AgsI TTSAA 8 cut(s) 436, 499, 1010, 1172, 1498, 1550, 1607, 1661
AjnI CCWGG 3 cut(s) 265, 406, 1437
AluBI AGCT 5 cut(s) 44, 72, 445, 734, 1069
AluI AGCT 5 cut(s) 44, 72, 445, 734, 1069
Alw21I GWGCWC 1 cut(s) 651
Alw26I GTCTC 1 cut(s) 1281
Alw44I GTGCAC 1 cut(s) 647
AlwNI CAGNNNCTG 1 cut(s) 430
AoxI GGCC 7 cut(s) 37, 51, 464, 663, 1095, 1110, 1585
ApaLI GTGCAC 1 cut(s) 647
ApeKI GCWGC 3 cut(s) 94, 326, 596
ApoI RAATTY 8 cut(s) 31, 127, 178, 436, 754, 784, 835, 1499
ArsI GACNNNNNNTTYG 2 cut(s) 1318, 1350
AsiGI ACCGGT 1 cut(s) 1308
Asp700I GAANNNNTTC 1 cut(s) 769
AspS9I GGNCC 7 cut(s) 158, 251, 293, 464, 554, 1306, 1585
AsuC2I CCSGG 3 cut(s) 50, 168, 1567
AsuHPI GGTGA 3 cut(s) 17, 38, 1468
AsuII TTCGAA 1 cut(s) 752
AvaII GGWCC 5 cut(s) 158, 251, 293, 554, 1306
AxyI CCTNAGG 1 cut(s) 591
BaeGI GKGCMC 2 cut(s) 228, 651
BbsI GAAGAC 1 cut(s) 1548
Bbv12I GWGCWC 1 cut(s) 651
BbvCI CCTCAGC 1 cut(s) 162
BbvI GCAGC 3 cut(s) 106, 313, 583
BccI CCATC 6 cut(s) 88, 124, 148, 1051, 1156, 1195
BceAI ACGGC 1 cut(s) 1572
BciT130I CCWGG 3 cut(s) 267, 408, 1439
BcnI CCSGG 3 cut(s) 50, 168, 1567
BcoDI GTCTC 1 cut(s) 1281
BfaI CTAG 1 cut(s) 1693
BfmI CTRYAG 2 cut(s) 597, 1388
BglI GCCNNNNNGGC 1 cut(s) 472
BglII AGATCT 1 cut(s) 974
BisI GCNGC 3 cut(s) 95, 327, 597
BlpI GCTNAGC 1 cut(s) 908
BlsI GCNGC 3 cut(s) 96, 328, 598
Bme1390I CCNGG 6 cut(s) 50, 168, 267, 408, 1439, 1567
Bme18I GGWCC 5 cut(s) 158, 251, 293, 554, 1306
BmgT120I GGNCC 7 cut(s) 158, 251, 293, 464, 554, 1306, 1585
BmiI GGNNCC 5 cut(s) 159, 244, 253, 1377, 1586
BmrFI CCNGG 6 cut(s) 50, 168, 267, 408, 1439, 1567
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 2 cut(s) 192, 805
BmuI ACTGGG 1 cut(s) 270
BpiI GAAGAC 1 cut(s) 1548
Bpu10I CCTNAGC 1 cut(s) 162
Bpu1102I GCTNAGC 1 cut(s) 908
Bpu14I TTCGAA 1 cut(s) 752
BpuEI CTTGAG 1 cut(s) 1118
BpuMI CCSGG 3 cut(s) 50, 168, 1567
BsaJI CCNNGG 3 cut(s) 247, 547, 706
BsaWI WCCGGW 1 cut(s) 1308
BsaXI ACNNNNNCTCC 4 cut(s) 1158, 1188, 1226, 1256
Bsc4I CCNNNNNNNGG 8 cut(s) 56, 155, 167, 281, 344, 638, 806, 824
Bse118I RCCGGY 2 cut(s) 53, 1308
Bse1I ACTGG 2 cut(s) 276, 518
Bse21I CCTNAGG 1 cut(s) 591
Bse3DI GCAATG 1 cut(s) 919
BseBI CCWGG 3 cut(s) 267, 408, 1439
BseDI CCNNGG 3 cut(s) 247, 547, 706
BseGI GGATG 1 cut(s) 796
BseLI CCNNNNNNNGG 8 cut(s) 56, 155, 167, 281, 344, 638, 806, 824
BseMI GCAATG 1 cut(s) 919
BseMII CTCAG 8 cut(s) 176, 275, 605, 854, 1104, 1149, 1170, 1215
BseNI ACTGG 2 cut(s) 276, 518
BseRI GAGGAG 2 cut(s) 75, 1247
BseSI GKGCMC 2 cut(s) 228, 651
BseX3I CGGCCG 1 cut(s) 51
BseXI GCAGC 3 cut(s) 106, 313, 583
Bsh1285I CGRYCG 1 cut(s) 54
BshFI GGCC 7 cut(s) 39, 53, 466, 665, 1097, 1112, 1587
BshTI ACCGGT 1 cut(s) 1308
BsiEI CGRYCG 1 cut(s) 54
BsiHKAI GWGCWC 1 cut(s) 651
BsiSI CCGG 7 cut(s) 36, 50, 54, 168, 666, 1309, 1566
BslFI GGGAC 3 cut(s) 171, 237, 724
BslI CCNNNNNNNGG 8 cut(s) 56, 155, 167, 281, 344, 638, 806, 824
BsmAI GTCTC 1 cut(s) 1281
BsmFI GGGAC 3 cut(s) 171, 237, 724
BsnI GGCC 7 cut(s) 39, 53, 466, 665, 1097, 1112, 1587
Bsp119I TTCGAA 1 cut(s) 752
Bsp1286I GDGCHC 2 cut(s) 228, 651
Bsp143I GATC 4 cut(s) 974, 1262, 1328, 1683
Bsp1720I GCTNAGC 1 cut(s) 908
BspACI CCGC 2 cut(s) 57, 818
BspANI GGCC 7 cut(s) 39, 53, 466, 665, 1097, 1112, 1587
BspCNI CTCAG 8 cut(s) 175, 274, 604, 853, 1103, 1150, 1171, 1214
BspHI TCATGA 1 cut(s) 174
BspLI GGNNCC 5 cut(s) 159, 244, 253, 1377, 1586
BspMAI CTGCAG 2 cut(s) 601, 1392
BspQI GCTCTTC 1 cut(s) 942
BspT104I TTCGAA 1 cut(s) 752
BsrDI GCAATG 1 cut(s) 919
BsrFI RCCGGY 2 cut(s) 53, 1308
BsrI ACTGG 2 cut(s) 276, 518
BssAI RCCGGY 2 cut(s) 53, 1308
BssECI CCNNGG 3 cut(s) 247, 547, 706
BssMI GATC 4 cut(s) 974, 1262, 1328, 1683
BssT1I CCWWGG 2 cut(s) 247, 547
Bst2UI CCWGG 3 cut(s) 267, 408, 1439
Bst4CI ACNGT 1 cut(s) 1014
Bst6I CTCTTC 4 cut(s) 942, 1222, 1545, 1668
BstAPI GCANNNNNTGC 1 cut(s) 103
BstBI TTCGAA 1 cut(s) 752
BstC8I GCNNGC 3 cut(s) 55, 544, 1155
BstF5I GGATG 1 cut(s) 796
BstKTI GATC 4 cut(s) 977, 1265, 1331, 1686
BstMAI GTCTC 1 cut(s) 1281
BstMBI GATC 4 cut(s) 974, 1262, 1328, 1683
BstMCI CGRYCG 1 cut(s) 54
BstMWI GCNNNNNNNGC 6 cut(s) 50, 103, 232, 472, 593, 623
BstNI CCWGG 3 cut(s) 267, 408, 1439
BstSCI CCNGG 6 cut(s) 48, 166, 265, 406, 1437, 1565
BstSFI CTRYAG 2 cut(s) 597, 1388
BstSLI GKGCMC 2 cut(s) 228, 651
BstV1I GCAGC 3 cut(s) 106, 313, 583
BstV2I GAAGAC 1 cut(s) 1548
BstX2I RGATCY 1 cut(s) 974
BstYI RGATCY 1 cut(s) 974
BstZI CGGCCG 1 cut(s) 51
Bsu36I CCTNAGG 1 cut(s) 591
BsuRI GGCC 7 cut(s) 39, 53, 466, 665, 1097, 1112, 1587
BtsCI GGATG 1 cut(s) 796
BtsIMutI CAGTG 2 cut(s) 283, 525
Cac8I GCNNGC 3 cut(s) 55, 544, 1155
CaiI CAGNNNCTG 1 cut(s) 430
CciI TCATGA 1 cut(s) 174
Cfr10I RCCGGY 2 cut(s) 53, 1308
Cfr13I GGNCC 7 cut(s) 158, 251, 293, 464, 554, 1306, 1585
CsiI ACCWGGT 1 cut(s) 406
CspAI ACCGGT 1 cut(s) 1308
CviAII CATG 6 cut(s) 132, 175, 229, 1245, 1453, 1600
DpnI GATC 4 cut(s) 976, 1264, 1330, 1685
DpnII GATC 4 cut(s) 974, 1262, 1328, 1683
DraI TTTAAA 1 cut(s) 1474
EaeI YGGCCR 2 cut(s) 37, 51
EagI CGGCCG 1 cut(s) 51
Eam1104I CTCTTC 4 cut(s) 942, 1222, 1545, 1668
EarI CTCTTC 4 cut(s) 942, 1222, 1545, 1668
EclXI CGGCCG 1 cut(s) 51
Eco130I CCWWGG 2 cut(s) 247, 547
Eco32I GATATC 1 cut(s) 1411
Eco47I GGWCC 5 cut(s) 158, 251, 293, 554, 1306
Eco52I CGGCCG 1 cut(s) 51
Eco57I CTGAAG 1 cut(s) 1542
Eco81I CCTNAGG 1 cut(s) 591
EcoO109I RGGNCCY 1 cut(s) 158
EcoRI GAATTC 2 cut(s) 754, 835
EcoRII CCWGG 3 cut(s) 265, 406, 1437
EcoRV GATATC 1 cut(s) 1411
EcoT14I CCWWGG 2 cut(s) 247, 547
EcoT22I ATGCAT 1 cut(s) 568
ErhI CCWWGG 2 cut(s) 247, 547
FaeI CATG 6 cut(s) 135, 178, 232, 1248, 1456, 1603
FaqI GGGAC 3 cut(s) 171, 237, 724
FatI CATG 6 cut(s) 131, 174, 228, 1244, 1452, 1599
FauNDI CATATG 2 cut(s) 216, 469
Fnu4HI GCNGC 3 cut(s) 95, 327, 597
FokI GGATG 1 cut(s) 783
Fsp4HI GCNGC 3 cut(s) 95, 327, 597
FspBI CTAG 1 cut(s) 1693
GluI GCNGC 3 cut(s) 95, 327, 597
HaeIII GGCC 7 cut(s) 39, 53, 466, 665, 1097, 1112, 1587
HapII CCGG 7 cut(s) 36, 50, 54, 168, 666, 1309, 1566
Hin1II CATG 6 cut(s) 135, 178, 232, 1248, 1456, 1603
HpaII CCGG 7 cut(s) 36, 50, 54, 168, 666, 1309, 1566
HphI GGTGA 3 cut(s) 17, 38, 1468
Hpy166II GTNNAC 3 cut(s) 296, 649, 745
Hpy188I TCNGA 9 cut(s) 201, 707, 760, 834, 1093, 1204, 1260, 1338, 1408
Hpy188III TCNNGA 4 cut(s) 175, 941, 1414, 1484
Hpy8I GTNNAC 3 cut(s) 296, 649, 745
HpyAV CCTTC 9 cut(s) 159, 913, 917, 1016, 1116, 1297, 1333, 1368, 1532
HpyCH4III ACNGT 1 cut(s) 1014
HpyCH4IV ACGT 2 cut(s) 699, 1027
HpyF10VI GCNNNNNNNGC 6 cut(s) 50, 103, 232, 472, 593, 623
HpySE526I ACGT 2 cut(s) 699, 1027
Hsp92II CATG 6 cut(s) 135, 178, 232, 1248, 1456, 1603
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
Kzo9I GATC 4 cut(s) 974, 1262, 1328, 1683
LguI GCTCTTC 1 cut(s) 942
LmnI GCTCC 3 cut(s) 82, 1126, 1375
Lsp1109I GCAGC 3 cut(s) 106, 313, 583
LweI GCATC 2 cut(s) 192, 805
MabI ACCWGGT 1 cut(s) 406
MaeI CTAG 1 cut(s) 1693
MaeII ACGT 2 cut(s) 699, 1027
MaeIII GTNAC 6 cut(s) 3, 1427, 1456, 1589, 1616, 1640
MalI GATC 4 cut(s) 976, 1264, 1330, 1685
MboI GATC 4 cut(s) 974, 1262, 1328, 1683
MfeI CAATTG 1 cut(s) 413
MflI RGATCY 1 cut(s) 974
MhlI GDGCHC 2 cut(s) 228, 651
MlyI GAGTC 3 cut(s) 938, 1082, 1562
MmeI TCCRAC 1 cut(s) 262
Mph1103I ATGCAT 1 cut(s) 568
MroNI GCCGGC 1 cut(s) 53
MroXI GAANNNNTTC 1 cut(s) 769
MseI TTAA 4 cut(s) 111, 342, 1473, 1518
MslI CAYNNNNRTG 2 cut(s) 219, 1647
MspA1I CMGCKG 1 cut(s) 445
MspI CCGG 7 cut(s) 36, 50, 54, 168, 666, 1309, 1566
MspR9I CCNGG 6 cut(s) 50, 168, 267, 408, 1439, 1567
MunI CAATTG 1 cut(s) 413
MvaI CCWGG 3 cut(s) 267, 408, 1439
MwoI GCNNNNNNNGC 6 cut(s) 50, 103, 232, 472, 593, 623
NaeI GCCGGC 1 cut(s) 55
NciI CCSGG 3 cut(s) 50, 168, 1567
NdeI CATATG 2 cut(s) 216, 469
NdeII GATC 4 cut(s) 974, 1262, 1328, 1683
NgoMIV GCCGGC 1 cut(s) 53
NlaIII CATG 6 cut(s) 135, 178, 232, 1248, 1456, 1603
NlaIV GGNNCC 5 cut(s) 159, 244, 253, 1377, 1586
NmuCI GTSAC 4 cut(s) 1427, 1456, 1589, 1640
NsiI ATGCAT 1 cut(s) 568
NspV TTCGAA 1 cut(s) 752
PagI TCATGA 1 cut(s) 174
PciSI GCTCTTC 1 cut(s) 942
PdiI GCCGGC 1 cut(s) 55
PdmI GAANNNNTTC 1 cut(s) 769
PfeI GAWTC 9 cut(s) 370, 451, 490, 505, 685, 952, 1141, 1205, 1343
PinAI ACCGGT 1 cut(s) 1308
PkrI GCNGC 3 cut(s) 96, 328, 598
PleI GAGTC 3 cut(s) 938, 1082, 1561
PpsI GAGTC 3 cut(s) 938, 1082, 1561
PpuMI RGGWCCY 1 cut(s) 158
PsiI TTATAA 2 cut(s) 791, 1629
Psp5II RGGWCCY 1 cut(s) 158
Psp6I CCWGG 3 cut(s) 265, 406, 1437
PspGI CCWGG 3 cut(s) 265, 406, 1437
PspN4I GGNNCC 5 cut(s) 159, 244, 253, 1377, 1586
PspPI GGNCC 7 cut(s) 158, 251, 293, 464, 554, 1306, 1585
PspPPI RGGWCCY 1 cut(s) 158
PstI CTGCAG 2 cut(s) 601, 1392
PstNI CAGNNNCTG 1 cut(s) 430
PsuI RGATCY 1 cut(s) 974
PvuII CAGCTG 1 cut(s) 445
RseI CAYNNNNRTG 2 cut(s) 219, 1647
SapI GCTCTTC 1 cut(s) 942
SaqAI TTAA 4 cut(s) 111, 342, 1473, 1518
SatI GCNGC 3 cut(s) 95, 327, 597
Sau3AI GATC 4 cut(s) 974, 1262, 1328, 1683
Sau96I GGNCC 7 cut(s) 158, 251, 293, 464, 554, 1306, 1585
SchI GAGTC 3 cut(s) 938, 1082, 1562
ScrFI CCNGG 6 cut(s) 50, 168, 267, 408, 1439, 1567
SduI GDGCHC 2 cut(s) 228, 651
SexAI ACCWGGT 1 cut(s) 406
SfaNI GCATC 2 cut(s) 192, 805
SfcI CTRYAG 2 cut(s) 597, 1388
SfuI TTCGAA 1 cut(s) 752
SinI GGWCC 5 cut(s) 158, 251, 293, 554, 1306
SmiMI CAYNNNNRTG 2 cut(s) 219, 1647
SmlI CTYRAG 1 cut(s) 1133
SmoI CTYRAG 1 cut(s) 1133
SsiI CCGC 2 cut(s) 57, 818
SspI AATATT 1 cut(s) 460
SspMI CTAG 1 cut(s) 1693
StyD4I CCNGG 6 cut(s) 48, 166, 265, 406, 1437, 1565
StyI CCWWGG 2 cut(s) 247, 547
TaaI ACNGT 1 cut(s) 1014
TaiI ACGT 2 cut(s) 702, 1030
TaqI TCGA 2 cut(s) 152, 752
TfiI GAWTC 9 cut(s) 370, 451, 490, 505, 685, 952, 1141, 1205, 1343
Tru1I TTAA 4 cut(s) 111, 342, 1473, 1518
Tru9I TTAA 4 cut(s) 111, 342, 1473, 1518
TscAI CASTG 2 cut(s) 283, 525
TseFI GTSAC 4 cut(s) 1427, 1456, 1589, 1640
TseI GCWGC 3 cut(s) 94, 326, 596
Tsp45I GTSAC 4 cut(s) 1427, 1456, 1589, 1640
TspGWI ACGGA 1 cut(s) 1010
TspRI CASTG 2 cut(s) 283, 525
VneI GTGCAC 1 cut(s) 647
VpaK11BI GGWCC 5 cut(s) 158, 251, 293, 554, 1306
XapI RAATTY 8 cut(s) 31, 127, 178, 436, 754, 784, 835, 1499
XmnI GAANNNNTTC 1 cut(s) 769
XspI CTAG 1 cut(s) 1693
Zsp2I ATGCAT 1 cut(s) 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.