MD02G1263300.v1.1

Nuclear fragile X mental retardation-interacting protein 1 (NUFIP1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005893
Physical Location & Seq
Reverse (-)
31760665 .. 31765976
5312 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1263300.v1.1.491

Sequence Viewer

Length: 1710 bp
ATGTTACCTCACTACAATTCCTTTCCTCGCCATTCCAACCAAGCTCACACGACCGGTATCTCGGCTCCACCTCCTCACCAGATGCAACCTCAATTGGGTATCAGGAATCCCCAAATTCCAGTCCAATTTAGTAATTCTAATTCCATGATAGCTCAACCCAATTTCATGAATCTACCAAACAACCTTCCTCCTATGCAAAATAACCATCTGGGTATGCCTCAGTTCGGTTCCGTTGGCCCTACATCTCAGCCGGGTCAGCCGAATGTTGGGTTTTTTGGTTCGCAAAGTAACGGGCAAGGTATGAACTCGGTTGGGCAATTTTGCAATTTGGTGCAGCAAGTGAATCAATTGATGTCTTCTCAAGTGCCTGGGCAGATATTTGGGCATAACATGTTAAATTTACAGCAACAGATGAACCCGAATATGGGTTTGCCGTATGGGCAGTTTTGTTTGCCGAATTCCATGCAAAACATGAATCAATTTGTGCCAATGCAAATGCAAAATCCATCGCAAGTCCGTCCTAATTATGCTTTCCAGGGATCAAATCAAGCACCTCGGGCCACAATGTCTCAAAATGTCCCATTGTTTGCAAATAACCAGGCTAATCTGCATTCTAATCAGGCAGGCCAGCAAGCTAACCAGAATCAGCAAAACTTGGGTCTACCTCCAGTGCAAGGGACACAGGGAAATAATACAAACAGTAATGGTGGTTACAATTCAAATTCCAATTGGAAACAATCACCAAGCAGAAACTTTACAAAAAATCCGAAGAATCACCAAGGGGGATTTCAGAATCCGCAGTCACATCATATGAAAAATGCAAAGGGAAAGTTCACTTTTCGTAATGGGCACAAAGGAAAAGGGTTAAGAAATGAGAGCGAAGGAAAGTTTGCTCTGGCCAACTCTTCAAACCAGGGCAAGCAAGGGAAAAGATCTCTTTCCTTGTCATATACGGAACAAGAGATTGAGCGGTGGCGTGAGGAACGTAGGAAGCACTACCCATCAAAATCCAACATAGAGAAGAAGATAAATGAAAAGATGATAAATTCAGAGATCATTGAAAGAGAGGCCAACATTCGACGAGAGCAACTCAAAGAAATTCTTACAAAGCAGGCTGAGTTGGGAGTTGAAGTTGCCGAAGTACCATCATACTACCTCGCAGATTCTAATCAAGGGCATGTAAAAGAAGATAATGAATCATTGACCAAGAAGGGGAGATTGGCAAACAAGTTTGGTAAGAGAGAAAAATATGATAGAAAGGATCGGTTTTCCAAGAGGCAAAGATCACATCAAAAGGGTTCGTCTAATAACCCTTCTTTCAACAAGATGGAGCCAAACAAAAGGGAGCCAACCTTGCTGCAGAAGCTTCTTAGTGCAGATATTAGGAGAGACAGAAGCCACCTGCTGCAGGCGTTTAGGTTCATGGTGGCAAACTCTTTCTTCAAAGATTGTCCAGACAAACCTTTGAAATTTCCTACTGTAGCAGTTAAAGAAAGCTCGGGTGAGGAAGAGGTGGCTGAAAAGTTGTCCTCACTTGCGGAGAAAGATGCTTTAAAGGGAAGCGATAAATCCATGGTTGGAAGAGTTTATAACAATCATTCTGGAAATAACAACGATGTCAGGAATTATGATGATAGCGGTGATGAAGATGATGGTGAAGGAGAGGGAACAGAGACACCTGGGGAGGAAGAGGAAGAAATCATAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko05164 map03015 map05164
Pfam Domains
Protein Families
NUFIP1

Protein Analysis

570

Amino Acids

63.91

Weight (kDa)

9.4

Isoelectric Point (pI)

55.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUFIP1 PF10453 304 - 347 7.5e-12 FMR1-interacting protein 1 (NUFIP1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1590
AarI CACCTGC 1 cut(s) 1410
Acc36I ACCTGC 1 cut(s) 1410
AccBSI CCGCTC 1 cut(s) 970
AccI GTMKAC 1 cut(s) 661
AciI CCGC 4 cut(s) 797, 970, 1538, 1638
AclWI GGATC 2 cut(s) 547, 1269
AcoI YGGCCR 1 cut(s) 897
AcsI RAATTY 7 cut(s) 114, 397, 457, 721, 1045, 1098, 1469
AfaI GTAC 1 cut(s) 1143
AfiI CCNNNNNNNGG 8 cut(s) 95, 224, 266, 424, 425, 674, 1212, 1408
AflIII ACRYGT 1 cut(s) 390
AgeI ACCGGT 1 cut(s) 53
AgsI TTSAA 7 cut(s) 720, 909, 1061, 1130, 1321, 1444, 1468
AjnI CCWGG 5 cut(s) 367, 534, 597, 912, 1678
AjuI GAANNNNNNNTTGG 4 cut(s) 771, 803, 1202, 1234
AluBI AGCT 5 cut(s) 44, 152, 635, 1366, 1497
AluI AGCT 5 cut(s) 44, 152, 635, 1366, 1497
Alw26I GTCTC 3 cut(s) 573, 1383, 1667
AlwI GGATC 2 cut(s) 547, 1269
Ama87I CYCGRG 2 cut(s) 555, 1498
AoxI GGCC 5 cut(s) 235, 558, 625, 897, 1068
ApeKI GCWGC 3 cut(s) 334, 1357, 1405
ApoI RAATTY 7 cut(s) 114, 397, 457, 721, 1045, 1098, 1469
ArsI GACNNNNNNTTYG 2 cut(s) 643, 675
AsiGI ACCGGT 1 cut(s) 53
AspS9I GGNCC 2 cut(s) 236, 558
AsuC2I CCSGG 1 cut(s) 252
AsuHPI GGTGA 6 cut(s) 68, 732, 767, 1514, 1652, 1667
AvaI CYCGRG 2 cut(s) 555, 1498
BaeGI GKGCMC 1 cut(s) 852
BalI TGGCCA 1 cut(s) 899
BbsI GAAGAC 1 cut(s) 348
BbvI GCAGC 3 cut(s) 346, 1344, 1392
BccI CCATC 6 cut(s) 213, 514, 1009, 1153, 1321, 1646
BceAI ACGGC 1 cut(s) 418
BcgI CGANNNNNNTGC 2 cut(s) 445, 479
BciT130I CCWGG 5 cut(s) 369, 536, 599, 914, 1680
BcnI CCSGG 1 cut(s) 252
BcoDI GTCTC 3 cut(s) 573, 1383, 1667
BfaI CTAG 1 cut(s) 1708
BfmI CTRYAG 3 cut(s) 1358, 1406, 1479
BfuAI ACCTGC 1 cut(s) 1410
BglI GCCNNNNNGGC 1 cut(s) 439
BglII AGATCT 1 cut(s) 932
BisI GCNGC 3 cut(s) 335, 1358, 1406
BlsI GCNGC 3 cut(s) 336, 1359, 1407
Bme1390I CCNGG 6 cut(s) 252, 369, 536, 599, 914, 1680
BmeT110I CYCGRG 2 cut(s) 555, 1498
BmgT120I GGNCC 2 cut(s) 236, 558
BmiI GGNNCC 4 cut(s) 66, 229, 1332, 1347
BmrFI CCNGG 6 cut(s) 252, 369, 536, 599, 914, 1680
BmsI GCATC 2 cut(s) 72, 1537
BpiI GAAGAC 1 cut(s) 348
BplI GAGNNNNNCTC 2 cut(s) 1074, 1106
BpmI CTGGAG 1 cut(s) 651
BpuEI CTTGAG 1 cut(s) 345
BpuMI CCSGG 1 cut(s) 252
BsaBI GATNNNNATC 1 cut(s) 1167
BsaJI CCNNGG 7 cut(s) 368, 535, 554, 778, 913, 1572, 1679
BsaWI WCCGGW 1 cut(s) 53
BsaXI ACNNNNNCTCC 2 cut(s) 1116, 1146
Bsc4I CCNNNNNNNGG 8 cut(s) 95, 224, 266, 424, 425, 674, 1212, 1408
Bse118I RCCGGY 1 cut(s) 53
Bse1I ACTGG 2 cut(s) 119, 668
Bse8I GATNNNNATC 1 cut(s) 1167
BseBI CCWGG 5 cut(s) 369, 536, 599, 914, 1680
BseDI CCNNGG 7 cut(s) 368, 535, 554, 778, 913, 1572, 1679
BseJI GATNNNNATC 1 cut(s) 1167
BseLI CCNNNNNNNGG 8 cut(s) 95, 224, 266, 424, 425, 674, 1212, 1408
BseMII CTCAG 3 cut(s) 233, 260, 1107
BseNI ACTGG 2 cut(s) 119, 668
BseRI GAGGAG 1 cut(s) 63
BseSI GKGCMC 1 cut(s) 852
BseXI GCAGC 3 cut(s) 346, 1344, 1392
BsgI GTGCAG 2 cut(s) 353, 1395
Bsh1285I CGRYCG 1 cut(s) 54
BshFI GGCC 5 cut(s) 237, 560, 627, 899, 1070
BshTI ACCGGT 1 cut(s) 53
BsiEI CGRYCG 1 cut(s) 54
BsiHKCI CYCGRG 2 cut(s) 555, 1498
BsiSI CCGG 2 cut(s) 54, 251
BslFI GGGAC 2 cut(s) 563, 691
BslI CCNNNNNNNGG 8 cut(s) 95, 224, 266, 424, 425, 674, 1212, 1408
BsmAI GTCTC 3 cut(s) 573, 1383, 1667
BsmFI GGGAC 2 cut(s) 563, 691
BsmI GAATGC 1 cut(s) 610
BsnI GGCC 5 cut(s) 237, 560, 627, 899, 1070
BsoBI CYCGRG 2 cut(s) 555, 1498
Bsp1286I GDGCHC 1 cut(s) 852
Bsp143I GATC 5 cut(s) 539, 932, 1053, 1261, 1283
Bsp19I CCATGG 1 cut(s) 1572
BspACI CCGC 4 cut(s) 797, 970, 1538, 1638
BspANI GGCC 5 cut(s) 237, 560, 627, 899, 1070
BspCNI CTCAG 3 cut(s) 232, 259, 1108
BspHI TCATGA 1 cut(s) 165
BspLI GGNNCC 4 cut(s) 66, 229, 1332, 1347
BspMAI CTGCAG 2 cut(s) 1362, 1410
BspMI ACCTGC 1 cut(s) 1410
BspPI GGATC 2 cut(s) 547, 1269
BsrBI CCGCTC 1 cut(s) 970
BsrFI RCCGGY 1 cut(s) 53
BsrI ACTGG 2 cut(s) 119, 668
BssAI RCCGGY 1 cut(s) 53
BssECI CCNNGG 7 cut(s) 368, 535, 554, 778, 913, 1572, 1679
BssMI GATC 5 cut(s) 539, 932, 1053, 1261, 1283
BssT1I CCWWGG 2 cut(s) 778, 1572
Bst2UI CCWGG 5 cut(s) 369, 536, 599, 914, 1680
Bst4CI ACNGT 2 cut(s) 701, 1480
Bst6I CTCTTC 4 cut(s) 910, 1503, 1576, 1683
BstC8I GCNNGC 6 cut(s) 625, 629, 633, 920, 1113, 1410
BstDEI CTNAG 4 cut(s) 219, 246, 1116, 1370
BstDSI CCRYGG 1 cut(s) 1572
BstENI CCTNNNNNAGG 1 cut(s) 1406
BstKTI GATC 5 cut(s) 542, 935, 1056, 1264, 1286
BstMAI GTCTC 3 cut(s) 573, 1383, 1667
BstMBI GATC 5 cut(s) 539, 932, 1053, 1261, 1283
BstMCI CGRYCG 1 cut(s) 54
BstMWI GCNNNNNNNGC 5 cut(s) 256, 439, 557, 1354, 1363
BstNI CCWGG 5 cut(s) 369, 536, 599, 914, 1680
BstNSI RCATGY 2 cut(s) 394, 1181
BstSCI CCNGG 6 cut(s) 250, 367, 534, 597, 912, 1678
BstSFI CTRYAG 3 cut(s) 1358, 1406, 1479
BstSLI GKGCMC 1 cut(s) 852
BstV1I GCAGC 3 cut(s) 346, 1344, 1392
BstV2I GAAGAC 1 cut(s) 348
BstX2I RGATCY 1 cut(s) 932
BstYI RGATCY 1 cut(s) 932
BsuRI GGCC 5 cut(s) 237, 560, 627, 899, 1070
BtgI CCRYGG 1 cut(s) 1572
BtgZI GCGATG 1 cut(s) 492
BtsIMutI CAGTG 1 cut(s) 675
BveI ACCTGC 1 cut(s) 1410
Cac8I GCNNGC 6 cut(s) 625, 629, 633, 920, 1113, 1410
CciI TCATGA 1 cut(s) 165
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 2 cut(s) 236, 558
Csp6I GTAC 1 cut(s) 1142
CspAI ACCGGT 1 cut(s) 53
CviAII CATG 8 cut(s) 145, 166, 391, 463, 472, 1178, 1423, 1573
CviQI GTAC 1 cut(s) 1142
DdeI CTNAG 4 cut(s) 219, 246, 1116, 1370
DpnI GATC 5 cut(s) 541, 934, 1055, 1263, 1285
DpnII GATC 5 cut(s) 539, 932, 1053, 1261, 1283
DraI TTTAAA 1 cut(s) 1554
EaeI YGGCCR 1 cut(s) 897
Eam1104I CTCTTC 4 cut(s) 910, 1503, 1576, 1683
EarI CTCTTC 4 cut(s) 910, 1503, 1576, 1683
Eco130I CCWWGG 2 cut(s) 778, 1572
Eco88I CYCGRG 2 cut(s) 555, 1498
EcoNI CCTNNNNNAGG 1 cut(s) 1406
EcoRI GAATTC 1 cut(s) 457
EcoRII CCWGG 5 cut(s) 367, 534, 597, 912, 1678
EcoT14I CCWWGG 2 cut(s) 778, 1572
ErhI CCWWGG 2 cut(s) 778, 1572
FaeI CATG 8 cut(s) 148, 169, 394, 466, 475, 1181, 1426, 1576
FalI AAGNNNNNCTT 2 cut(s) 1086, 1118
FaqI GGGAC 2 cut(s) 563, 691
FatI CATG 8 cut(s) 144, 165, 390, 462, 471, 1177, 1422, 1572
FauNDI CATATG 1 cut(s) 810
FblI GTMKAC 1 cut(s) 661
Fnu4HI GCNGC 3 cut(s) 335, 1358, 1406
Fsp4HI GCNGC 3 cut(s) 335, 1358, 1406
FspBI CTAG 1 cut(s) 1708
GluI GCNGC 3 cut(s) 335, 1358, 1406
GsuI CTGGAG 1 cut(s) 651
HaeIII GGCC 5 cut(s) 237, 560, 627, 899, 1070
HapII CCGG 2 cut(s) 54, 251
Hin1II CATG 8 cut(s) 148, 169, 394, 466, 475, 1181, 1426, 1576
HindIII AAGCTT 1 cut(s) 1364
HinfI GANTC 9 cut(s) 106, 169, 343, 475, 643, 772, 793, 1163, 1196
HpaII CCGG 2 cut(s) 54, 251
HphI GGTGA 6 cut(s) 68, 732, 767, 1514, 1652, 1667
Hpy166II GTNNAC 2 cut(s) 662, 834
Hpy188I TCNGA 3 cut(s) 768, 792, 1051
Hpy188III TCNNGA 5 cut(s) 103, 166, 1454, 1602, 1621
Hpy8I GTNNAC 2 cut(s) 662, 834
Hpy99I CGWCG 1 cut(s) 1083
HpyAV CCTTC 5 cut(s) 194, 875, 1204, 1323, 1652
HpyCH4III ACNGT 2 cut(s) 701, 1480
HpyCH4IV ACGT 1 cut(s) 985
HpyF10VI GCNNNNNNNGC 5 cut(s) 256, 439, 557, 1354, 1363
HpyF3I CTNAG 4 cut(s) 219, 246, 1116, 1370
HpySE526I ACGT 1 cut(s) 985
Hsp92II CATG 8 cut(s) 148, 169, 394, 466, 475, 1181, 1426, 1576
Kzo9I GATC 5 cut(s) 539, 932, 1053, 1261, 1283
LmnI GCTCC 3 cut(s) 70, 1330, 1345
Lsp1109I GCAGC 3 cut(s) 346, 1344, 1392
LweI GCATC 2 cut(s) 72, 1537
MaeI CTAG 1 cut(s) 1708
MaeII ACGT 1 cut(s) 985
MaeIII GTNAC 4 cut(s) 3, 287, 710, 801
MalI GATC 5 cut(s) 541, 934, 1055, 1263, 1285
MbiI CCGCTC 1 cut(s) 970
MboI GATC 5 cut(s) 539, 932, 1053, 1261, 1283
MfeI CAATTG 3 cut(s) 92, 347, 727
MflI RGATCY 1 cut(s) 932
MhlI GDGCHC 1 cut(s) 852
MlsI TGGCCA 1 cut(s) 899
MluNI TGGCCA 1 cut(s) 899
MmeI TCCRAC 3 cut(s) 60, 1035, 1558
Mox20I TGGCCA 1 cut(s) 899
MscI TGGCCA 1 cut(s) 899
MseI TTAA 4 cut(s) 395, 866, 1488, 1553
Msp20I TGGCCA 1 cut(s) 899
MspI CCGG 2 cut(s) 54, 251
MspR9I CCNGG 6 cut(s) 252, 369, 536, 599, 914, 1680
MunI CAATTG 3 cut(s) 92, 347, 727
Mva1269I GAATGC 1 cut(s) 610
MvaI CCWGG 5 cut(s) 369, 536, 599, 914, 1680
MwoI GCNNNNNNNGC 5 cut(s) 256, 439, 557, 1354, 1363
NciI CCSGG 1 cut(s) 252
NcoI CCATGG 1 cut(s) 1572
NdeI CATATG 1 cut(s) 810
NdeII GATC 5 cut(s) 539, 932, 1053, 1261, 1283
NlaIII CATG 8 cut(s) 148, 169, 394, 466, 475, 1181, 1426, 1576
NlaIV GGNNCC 4 cut(s) 66, 229, 1332, 1347
NmeAIII GCCGAG 1 cut(s) 41
NmuCI GTSAC 1 cut(s) 801
NspI RCATGY 2 cut(s) 394, 1181
PagI TCATGA 1 cut(s) 165
PaqCI CACCTGC 1 cut(s) 1410
PciI ACATGT 1 cut(s) 390
PctI GAATGC 1 cut(s) 610
PfeI GAWTC 9 cut(s) 106, 169, 343, 475, 643, 772, 793, 1163, 1196
PinAI ACCGGT 1 cut(s) 53
PkrI GCNGC 3 cut(s) 336, 1359, 1407
PscI ACATGT 1 cut(s) 390
PsiI TTATAA 1 cut(s) 1590
Psp6I CCWGG 5 cut(s) 367, 534, 597, 912, 1678
PspGI CCWGG 5 cut(s) 367, 534, 597, 912, 1678
PspN4I GGNNCC 4 cut(s) 66, 229, 1332, 1347
PspPI GGNCC 2 cut(s) 236, 558
PstI CTGCAG 2 cut(s) 1362, 1410
PsuI RGATCY 1 cut(s) 932
RsaI GTAC 1 cut(s) 1143
RsaNI GTAC 1 cut(s) 1142
SaqAI TTAA 4 cut(s) 395, 866, 1488, 1553
SatI GCNGC 3 cut(s) 335, 1358, 1406
Sau3AI GATC 5 cut(s) 539, 932, 1053, 1261, 1283
Sau96I GGNCC 2 cut(s) 236, 558
ScrFI CCNGG 6 cut(s) 252, 369, 536, 599, 914, 1680
SduI GDGCHC 1 cut(s) 852
SfaNI GCATC 2 cut(s) 72, 1537
SfcI CTRYAG 3 cut(s) 1358, 1406, 1479
SmlI CTYRAG 1 cut(s) 360
SmoI CTYRAG 1 cut(s) 360
SsiI CCGC 4 cut(s) 797, 970, 1538, 1638
SspMI CTAG 1 cut(s) 1708
StyD4I CCNGG 6 cut(s) 250, 367, 534, 597, 912, 1678
StyI CCWWGG 2 cut(s) 778, 1572
TaaI ACNGT 2 cut(s) 701, 1480
TaiI ACGT 1 cut(s) 988
TaqI TCGA 1 cut(s) 1078
TfiI GAWTC 9 cut(s) 106, 169, 343, 475, 643, 772, 793, 1163, 1196
Tru1I TTAA 4 cut(s) 395, 866, 1488, 1553
Tru9I TTAA 4 cut(s) 395, 866, 1488, 1553
TscAI CASTG 1 cut(s) 675
TseFI GTSAC 1 cut(s) 801
TseI GCWGC 3 cut(s) 334, 1357, 1405
Tsp45I GTSAC 1 cut(s) 801
TspGWI ACGGA 3 cut(s) 220, 506, 968
TspRI CASTG 1 cut(s) 675
XagI CCTNNNNNAGG 1 cut(s) 1406
XapI RAATTY 7 cut(s) 114, 397, 457, 721, 1045, 1098, 1469
XceI RCATGY 2 cut(s) 394, 1181
XmiI GTMKAC 1 cut(s) 661
XspI CTAG 1 cut(s) 1708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.