MD07G1054700.v1.1

Nuclear fragile X mental retardation-interacting protein 1 (NUFIP1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005893
Physical Location & Seq
Forward (+)
5058703 .. 5063991
5289 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1054700.v1.1.491

Sequence Viewer

Length: 1692 bp
ATGTTACCTCACTACAATTCGTTTCCTCGCCATTCAAACCAAGCTCACACAACTGGTAGCTTGGCTCCACCTCCTCACCAGATGCAACCTCAATTGGGTATCAGGAATCCCCAAATTCCAGTCCAATTTAGTAATTCCAATTCCATGATAGCTCTACCCAATTTCATGAATCTACCAAACAACCTTCTTCCTATGCAAAATAACCATCTGGGTATGCCTCAATTCGGTTCCATTGGCCCTACATCTCAACCGGGTCAGCCGAATGTTGGGTTTTTCGGTTCGCAAGGTATGCCTCAATTCTGCAATTTGGTGCAGCAAGTGAATCAATTGATGTCATCTCAAGTGCCTGGGCAAATATTTGGGCATAACATGTTAAATTTACAGCAACAGATGAACCAGAATATGGGTTTGCCGTATGGACAATTTTGTTTGCCCAATTCAATGCAAAACATGAATCAATTTGTGCCAATGCAAATGCAAAATCCACCACAAGTCCGTCCTAATTATGGTTTTCCGGTATCAAATCAAGCACCTCAGACCACAATGTCTCAAAATGTCCCATTGTTTGCAAATAACCGGGCTAATCTGCATTCTAATCAGGCAGGCCAGCAAGTTAACCAAAATCAGCAAAACTTGGGTCTACCCCCAGTGCAAGGGACACAGGAAAATAAAACAAATAGTAGTGGTGGTTACAATTCAAATTCCAATTGGAAACAATCACCAAGCAAAAGCTTTACAAAGAATCCGAAGAATCACCAAGGCGGATTTCAGAATCCTCAGGCGCATCATATGAAAAATGCAAAGGGAAAGTTCTCTTTTCCTAATGGGCACAAGGGAAAAGGGTTAAGAAATGAGAGTGAAGGAAAGTTTTCTCTGGCCAACTCTTCAAACCAGGGCAGGGAAAGGAAGAGAAGATCTCTTTTCTTGTCATATACGGAACAAGAGATTGAGAGGTGGCGGGAAGAACGTAGGAAGCACTACCCATCGAAATCCAACATAGAGAAGAAGATAAGTGAAAAGCTGATAAATTCAGAGGTCATTGAAAGAGAGGTCAACATTCGACGAGAGCAACTCAAGGATATTCTTACAAAGCAGGCTGAGTTGGGAGTTCAAATTGCCGAAGTACCATCGCACTACCTCGCAGATTCAAATCAAGGGCATATAAAAGAAGATAATGAAACATTGACCAAGAAGGGGAGGTCATCAAACAAGTTTGGTAAGAGAGAAAAATATGATAGAAAGGATCGGTTTTCCAAGAGGCAAAGATCACATCAAAGGGATTCGTCTAGTGACCCTTCTTTTGACAAAAGGGAGCCAAACAAAAGGGAGCCAACCTTGCTGCAGAAGCTTCTTAGTGCAGATATTAGGAGAGACAGAAGCCACCTGCTGCAGGTGTTTAGGTTCATGGTTACAAACTCTTTCTTCAAAGATTGTCCAGACAAACCTTTGAAATTCCCTACTGTAGCGGTAAAAGAAAGTTCGTGTGACGAAGAGGTGGCTCAAAAGTTGTCCTCACTTGTAGAGAAAGATGCTTTTAAGGGAAGTGATAAATCCATGGTTGGAAGAGTTTATAACAATCATTCTGGAAATAACAACGATGTCGGGAATTCTGTTGATAGCAGTGATGAAGATGATGGTGAAGGAGAGGGAACAGAGAGACCTGGGGAAGAAGAGGAAGAAATCATAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko05164 map03015 map05164
Pfam Domains
Protein Families
NUFIP1

Protein Analysis

564

Amino Acids

63.74

Weight (kDa)

9.38

Isoelectric Point (pI)

59.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUFIP1 PF10453 310 - 341 5.2e-11 FMR1-interacting protein 1 (NUFIP1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1572
AarI CACCTGC 2 cut(s) 1381, 1392
AasI GACNNNNNNGTC 1 cut(s) 544
Acc36I ACCTGC 2 cut(s) 1381, 1392
AccB7I CCANNNNNTGG 1 cut(s) 403
AccI GTMKAC 1 cut(s) 640
AciI CCGC 3 cut(s) 762, 958, 1466
AclWI GGATC 1 cut(s) 1251
AcoI YGGCCR 1 cut(s) 876
AcsI RAATTY 6 cut(s) 114, 376, 700, 1027, 1451, 1606
AfaI GTAC 1 cut(s) 1125
AfiI CCNNNNNNNGG 9 cut(s) 95, 224, 266, 403, 506, 653, 898, 1194, 1390
AflIII ACRYGT 1 cut(s) 369
AgsI TTSAA 9 cut(s) 36, 441, 699, 888, 1043, 1112, 1149, 1426, 1450
AjnI CCWGG 3 cut(s) 346, 891, 1660
AjuI GAANNNNNNNTTGG 2 cut(s) 750, 782
AluBI AGCT 6 cut(s) 44, 60, 152, 732, 1021, 1348
AluI AGCT 6 cut(s) 44, 60, 152, 732, 1021, 1348
Alw26I GTCTC 3 cut(s) 552, 1365, 1651
AlwI GGATC 1 cut(s) 1251
AoxI GGCC 3 cut(s) 235, 604, 876
ApeKI GCWGC 3 cut(s) 313, 1339, 1387
ApoI RAATTY 6 cut(s) 114, 376, 700, 1027, 1451, 1606
ArsI GACNNNNNNTTYG 4 cut(s) 622, 654, 1283, 1315
Asp700I GAANNNNTTC 1 cut(s) 868
AspLEI GCGC 1 cut(s) 784
AspS9I GGNCC 1 cut(s) 236
AsuC2I CCSGG 2 cut(s) 252, 578
AsuHPI GGTGA 4 cut(s) 68, 711, 746, 1649
AxyI CCTNAGG 1 cut(s) 777
BaeGI GKGCMC 1 cut(s) 831
BalI TGGCCA 1 cut(s) 878
BbvI GCAGC 3 cut(s) 325, 1326, 1374
BccI CCATC 4 cut(s) 213, 991, 1135, 1628
BceAI ACGGC 1 cut(s) 397
BciT130I CCWGG 3 cut(s) 348, 893, 1662
BcnI CCSGG 2 cut(s) 252, 578
BcoDI GTCTC 3 cut(s) 552, 1365, 1651
BfaI CTAG 2 cut(s) 1287, 1690
BfmI CTRYAG 3 cut(s) 1340, 1388, 1461
BfuAI ACCTGC 2 cut(s) 1381, 1392
BglII AGATCT 1 cut(s) 914
BisI GCNGC 3 cut(s) 314, 1340, 1388
BlsI GCNGC 3 cut(s) 315, 1341, 1389
Bme1390I CCNGG 5 cut(s) 252, 348, 578, 893, 1662
BmgT120I GGNCC 1 cut(s) 236
BmiI GGNNCC 4 cut(s) 66, 229, 1314, 1329
BmrFI CCNGG 5 cut(s) 252, 348, 578, 893, 1662
BmrI ACTGGG 1 cut(s) 641
BmsI GCATC 3 cut(s) 72, 793, 1519
BmuI ACTGGG 1 cut(s) 641
BplI GAGNNNNNCTC 4 cut(s) 901, 933, 1056, 1088
BpuEI CTTGAG 2 cut(s) 324, 1058
BpuMI CCSGG 2 cut(s) 252, 578
BsaBI GATNNNNATC 1 cut(s) 1149
BsaI GGTCTC 1 cut(s) 1651
BsaJI CCNNGG 5 cut(s) 347, 757, 892, 1554, 1661
BsaWI WCCGGW 1 cut(s) 514
Bsc4I CCNNNNNNNGG 9 cut(s) 95, 224, 266, 403, 506, 653, 898, 1194, 1390
Bse1I ACTGG 3 cut(s) 58, 119, 647
Bse21I CCTNAGG 1 cut(s) 777
Bse8I GATNNNNATC 1 cut(s) 1149
BseBI CCWGG 3 cut(s) 348, 893, 1662
BseDI CCNNGG 5 cut(s) 347, 757, 892, 1554, 1661
BseJI GATNNNNATC 1 cut(s) 1149
BseLI CCNNNNNNNGG 9 cut(s) 95, 224, 266, 403, 506, 653, 898, 1194, 1390
BseMII CTCAG 3 cut(s) 548, 791, 1089
BseNI ACTGG 3 cut(s) 58, 119, 647
BseRI GAGGAG 1 cut(s) 63
BseSI GKGCMC 1 cut(s) 831
BseXI GCAGC 3 cut(s) 325, 1326, 1374
BsgI GTGCAG 2 cut(s) 332, 1377
BshFI GGCC 3 cut(s) 237, 606, 878
BsiSI CCGG 3 cut(s) 251, 515, 577
BslFI GGGAC 2 cut(s) 542, 670
BslI CCNNNNNNNGG 9 cut(s) 95, 224, 266, 403, 506, 653, 898, 1194, 1390
BsmAI GTCTC 3 cut(s) 552, 1365, 1651
BsmFI GGGAC 2 cut(s) 542, 670
BsmI GAATGC 1 cut(s) 589
BsnI GGCC 3 cut(s) 237, 606, 878
Bso31I GGTCTC 1 cut(s) 1651
Bsp1286I GDGCHC 1 cut(s) 831
Bsp143I GATC 3 cut(s) 914, 1243, 1265
Bsp19I CCATGG 1 cut(s) 1554
BspACI CCGC 3 cut(s) 762, 958, 1466
BspANI GGCC 3 cut(s) 237, 606, 878
BspCNI CTCAG 3 cut(s) 547, 790, 1090
BspHI TCATGA 1 cut(s) 165
BspLI GGNNCC 4 cut(s) 66, 229, 1314, 1329
BspMAI CTGCAG 2 cut(s) 1344, 1392
BspMI ACCTGC 2 cut(s) 1381, 1392
BspPI GGATC 1 cut(s) 1251
BspTNI GGTCTC 1 cut(s) 1651
BsrI ACTGG 3 cut(s) 58, 119, 647
BssECI CCNNGG 5 cut(s) 347, 757, 892, 1554, 1661
BssMI GATC 3 cut(s) 914, 1243, 1265
BssT1I CCWWGG 2 cut(s) 757, 1554
Bst2UI CCWGG 3 cut(s) 348, 893, 1662
Bst4CI ACNGT 1 cut(s) 1462
Bst6I CTCTTC 5 cut(s) 889, 902, 1485, 1558, 1665
BstAPI GCANNNNNTGC 1 cut(s) 289
BstC8I GCNNGC 3 cut(s) 604, 608, 1095
BstDEI CTNAG 4 cut(s) 534, 777, 1098, 1352
BstDSI CCRYGG 1 cut(s) 1554
BstENI CCTNNNNNAGG 1 cut(s) 1388
BstHHI GCGC 1 cut(s) 784
BstKTI GATC 3 cut(s) 917, 1246, 1268
BstMAI GTCTC 3 cut(s) 552, 1365, 1651
BstMBI GATC 3 cut(s) 914, 1243, 1265
BstMWI GCNNNNNNNGC 3 cut(s) 289, 1336, 1345
BstNI CCWGG 3 cut(s) 348, 893, 1662
BstNSI RCATGY 1 cut(s) 373
BstSCI CCNGG 5 cut(s) 250, 346, 576, 891, 1660
BstSFI CTRYAG 3 cut(s) 1340, 1388, 1461
BstSLI GKGCMC 1 cut(s) 831
BstV1I GCAGC 3 cut(s) 325, 1326, 1374
BstX2I RGATCY 1 cut(s) 914
BstYI RGATCY 1 cut(s) 914
Bsu36I CCTNAGG 1 cut(s) 777
BsuRI GGCC 3 cut(s) 237, 606, 878
BtgI CCRYGG 1 cut(s) 1554
BtgZI GCGATG 1 cut(s) 1113
BtsI GCAGTG 1 cut(s) 1627
BtsIMutI CAGTG 2 cut(s) 654, 1627
BveI ACCTGC 2 cut(s) 1381, 1392
Cac8I GCNNGC 3 cut(s) 604, 608, 1095
CciI TCATGA 1 cut(s) 165
CfoI GCGC 1 cut(s) 784
Cfr13I GGNCC 1 cut(s) 236
Csp6I GTAC 1 cut(s) 1124
CviAII CATG 6 cut(s) 145, 166, 370, 451, 1405, 1555
CviQI GTAC 1 cut(s) 1124
DdeI CTNAG 4 cut(s) 534, 777, 1098, 1352
DpnI GATC 3 cut(s) 916, 1245, 1267
DpnII GATC 3 cut(s) 914, 1243, 1265
DrdI GACNNNNNNGTC 1 cut(s) 544
DseDI GACNNNNNNGTC 1 cut(s) 544
EaeI YGGCCR 1 cut(s) 876
Eam1104I CTCTTC 5 cut(s) 889, 902, 1485, 1558, 1665
EarI CTCTTC 5 cut(s) 889, 902, 1485, 1558, 1665
EciI GGCGGA 1 cut(s) 777
Eco130I CCWWGG 2 cut(s) 757, 1554
Eco31I GGTCTC 1 cut(s) 1651
Eco81I CCTNAGG 1 cut(s) 777
EcoNI CCTNNNNNAGG 1 cut(s) 1388
EcoRI GAATTC 1 cut(s) 1606
EcoRII CCWGG 3 cut(s) 346, 891, 1660
EcoT14I CCWWGG 2 cut(s) 757, 1554
ErhI CCWWGG 2 cut(s) 757, 1554
FaeI CATG 6 cut(s) 148, 169, 373, 454, 1408, 1558
FaqI GGGAC 2 cut(s) 542, 670
FatI CATG 6 cut(s) 144, 165, 369, 450, 1404, 1554
FauI CCCGC 1 cut(s) 951
FauNDI CATATG 1 cut(s) 789
FblI GTMKAC 1 cut(s) 640
Fnu4HI GCNGC 3 cut(s) 314, 1340, 1388
Fsp4HI GCNGC 3 cut(s) 314, 1340, 1388
FspBI CTAG 2 cut(s) 1287, 1690
GlaI GCGC 1 cut(s) 783
GluI GCNGC 3 cut(s) 314, 1340, 1388
HaeIII GGCC 3 cut(s) 237, 606, 878
HapII CCGG 3 cut(s) 251, 515, 577
HhaI GCGC 1 cut(s) 784
Hin1II CATG 6 cut(s) 148, 169, 373, 454, 1408, 1558
Hin6I GCGC 1 cut(s) 782
HinP1I GCGC 1 cut(s) 782
HincII GTYRAC 2 cut(s) 616, 1054
HindII GTYRAC 2 cut(s) 616, 1054
HindIII AAGCTT 2 cut(s) 730, 1346
HinfI GANTC 9 cut(s) 106, 169, 322, 454, 742, 751, 772, 1145, 1280
HpaI GTTAAC 1 cut(s) 616
HpaII CCGG 3 cut(s) 251, 515, 577
HphI GGTGA 4 cut(s) 68, 711, 746, 1649
Hpy166II GTNNAC 3 cut(s) 616, 641, 1054
Hpy188I TCNGA 4 cut(s) 537, 747, 771, 1033
Hpy188III TCNNGA 5 cut(s) 103, 166, 1436, 1584, 1603
Hpy8I GTNNAC 3 cut(s) 616, 641, 1054
Hpy99I CGWCG 1 cut(s) 1065
HpyAV CCTTC 5 cut(s) 194, 854, 1186, 1305, 1634
HpyCH4III ACNGT 1 cut(s) 1462
HpyCH4IV ACGT 1 cut(s) 967
HpyF10VI GCNNNNNNNGC 3 cut(s) 289, 1336, 1345
HpyF3I CTNAG 4 cut(s) 534, 777, 1098, 1352
HpySE526I ACGT 1 cut(s) 967
Hsp92II CATG 6 cut(s) 148, 169, 373, 454, 1408, 1558
HspAI GCGC 1 cut(s) 782
KspAI GTTAAC 1 cut(s) 616
Kzo9I GATC 3 cut(s) 914, 1243, 1265
LmnI GCTCC 3 cut(s) 70, 1312, 1327
Lsp1109I GCAGC 3 cut(s) 325, 1326, 1374
LweI GCATC 3 cut(s) 72, 793, 1519
MaeI CTAG 2 cut(s) 1287, 1690
MaeII ACGT 1 cut(s) 967
MaeIII GTNAC 5 cut(s) 3, 689, 1289, 1408, 1484
MalI GATC 3 cut(s) 916, 1245, 1267
MboI GATC 3 cut(s) 914, 1243, 1265
MfeI CAATTG 3 cut(s) 92, 326, 706
MflI RGATCY 1 cut(s) 914
MhlI GDGCHC 1 cut(s) 831
MlsI TGGCCA 1 cut(s) 878
MluNI TGGCCA 1 cut(s) 878
MmeI TCCRAC 2 cut(s) 1017, 1540
Mox20I TGGCCA 1 cut(s) 878
MroXI GAANNNNTTC 1 cut(s) 868
MscI TGGCCA 1 cut(s) 878
MseI TTAA 4 cut(s) 374, 615, 845, 1536
Msp20I TGGCCA 1 cut(s) 878
MspI CCGG 3 cut(s) 251, 515, 577
MspR9I CCNGG 5 cut(s) 252, 348, 578, 893, 1662
MunI CAATTG 3 cut(s) 92, 326, 706
Mva1269I GAATGC 1 cut(s) 589
MvaI CCWGG 3 cut(s) 348, 893, 1662
MwoI GCNNNNNNNGC 3 cut(s) 289, 1336, 1345
NciI CCSGG 2 cut(s) 252, 578
NcoI CCATGG 1 cut(s) 1554
NdeI CATATG 1 cut(s) 789
NdeII GATC 3 cut(s) 914, 1243, 1265
NlaIII CATG 6 cut(s) 148, 169, 373, 454, 1408, 1558
NlaIV GGNNCC 4 cut(s) 66, 229, 1314, 1329
NmuCI GTSAC 2 cut(s) 1289, 1484
NspI RCATGY 1 cut(s) 373
PagI TCATGA 1 cut(s) 165
PaqCI CACCTGC 2 cut(s) 1381, 1392
PciI ACATGT 1 cut(s) 369
PctI GAATGC 1 cut(s) 589
PdmI GAANNNNTTC 1 cut(s) 868
PfeI GAWTC 9 cut(s) 106, 169, 322, 454, 742, 751, 772, 1145, 1280
PflMI CCANNNNNTGG 1 cut(s) 403
PkrI GCNGC 3 cut(s) 315, 1341, 1389
PscI ACATGT 1 cut(s) 369
PsiI TTATAA 1 cut(s) 1572
Psp6I CCWGG 3 cut(s) 346, 891, 1660
PspGI CCWGG 3 cut(s) 346, 891, 1660
PspN4I GGNNCC 4 cut(s) 66, 229, 1314, 1329
PspPI GGNCC 1 cut(s) 236
PstI CTGCAG 2 cut(s) 1344, 1392
PsuI RGATCY 1 cut(s) 914
RsaI GTAC 1 cut(s) 1125
RsaNI GTAC 1 cut(s) 1124
SaqAI TTAA 4 cut(s) 374, 615, 845, 1536
SatI GCNGC 3 cut(s) 314, 1340, 1388
Sau3AI GATC 3 cut(s) 914, 1243, 1265
Sau96I GGNCC 1 cut(s) 236
ScrFI CCNGG 5 cut(s) 252, 348, 578, 893, 1662
SduI GDGCHC 1 cut(s) 831
SfaNI GCATC 3 cut(s) 72, 793, 1519
SfcI CTRYAG 3 cut(s) 1340, 1388, 1461
SmlI CTYRAG 2 cut(s) 339, 1073
SmoI CTYRAG 2 cut(s) 339, 1073
SsiI CCGC 3 cut(s) 762, 958, 1466
SspI AATATT 1 cut(s) 357
SspMI CTAG 2 cut(s) 1287, 1690
StyD4I CCNGG 5 cut(s) 250, 346, 576, 891, 1660
StyI CCWWGG 2 cut(s) 757, 1554
TaaI ACNGT 1 cut(s) 1462
TaiI ACGT 1 cut(s) 970
TaqI TCGA 2 cut(s) 986, 1060
TfiI GAWTC 9 cut(s) 106, 169, 322, 454, 742, 751, 772, 1145, 1280
Tru1I TTAA 4 cut(s) 374, 615, 845, 1536
Tru9I TTAA 4 cut(s) 374, 615, 845, 1536
TscAI CASTG 2 cut(s) 654, 1627
TseFI GTSAC 2 cut(s) 1289, 1484
TseI GCWGC 3 cut(s) 313, 1339, 1387
Tsp45I GTSAC 2 cut(s) 1289, 1484
TspDTI ATGAA 8 cut(s) 154, 182, 407, 467, 806, 1191, 1393, 1641
TspGWI ACGGA 2 cut(s) 485, 950
TspRI CASTG 2 cut(s) 654, 1627
Van91I CCANNNNNTGG 1 cut(s) 403
XagI CCTNNNNNAGG 1 cut(s) 1388
XapI RAATTY 6 cut(s) 114, 376, 700, 1027, 1451, 1606
XceI RCATGY 1 cut(s) 373
XmiI GTMKAC 1 cut(s) 640
XmnI GAANNNNTTC 1 cut(s) 868
XspI CTAG 2 cut(s) 1287, 1690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.