pycom02g22570

Nuclear fragile X mental retardation-interacting protein 1 (NUFIP1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0005893
Physical Location & Seq
Reverse (-)
20696446 .. 20701674
5229 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g22570.1

Sequence Viewer

Length: 1695 bp
ATGTTACCTCACTACAATTCCTTTCCTCGCCATTCCAACCAAGCTCACACGACGGGTATCTCGGCTCCACCTCCTCACCAGATGCAACCTCAATTGGGTATCAGGAATCCCCAAATTCCAGTCCAATTTAGTACTAACAATTCCATGATAGCTCAACCCAATTTCATGAATCTACCAAACAATCTTCTTCCTATGCAAAATAACCATCTGGGTAGGCCTCAGTTCGGTTCCGTTGGCCCTACATCTCAACCGGGTCAGCCGAATGTTGGGTTTTTTGGTTCGCAAAGTAACGGGCAAGGTATGAACTCGGTTGGGCAATTTTGCAATTTGGTGCAGCAAGTGAATCAATTGATGTCATCTCAAGTGCCTGGGCAGATATTTGGGCATAACATGTTAAATTTACAGCAACAGATGAACCCGAATATGGGTTTACCGTATGGGCAGTTTTGTTTGCCGAATTCAATGCAAAACATGAATCAATTTGTGCCAATGCAAATGCAAAATCCATCGCAAGTCCGTCCTAATTATGCTTTTCCGGGATCAAATCAACCACCTCGGGCCACAATGTCTCAAAATGTCCCATTGTTTGCAAATAACCAGGCTAATCTGCATTCTAATCAGGCAGGCCAGCAAGCTAACCAGAATCAGCAAAACTTGGGTCTACCTCCAGTGCAAGGGACACAGGGAAATAATTCAAACAGTAATGGTGGTTACAATTCAAATTCCAATTGGAAACAATCACCAAGCAGAAACTTTGCAAAAAATCAGACGAATCACCAAGGGGGATTTCAGAATCCTCAGTCACATCATATGAAAAATGCAAAGGGAAAGTTCACTTTTCGTAATGGGCACAAAGGAAAAGGGTTAAGAAATGAGAGCGAAGGAAAGTTTGCTCTGGCCAACTCTTCAAACCAGGGCAAGCAAGGGAAGAGATCTCTTTCCTTGTCATATATGGAACAAGAGATTGAGCGGTGGCGTGAAGAACGTAGGAAGCACTACCCATCAAAATCCAACATAGAGAAGAAGATAAGTGAAAAGATGATAAATTCAGAGGTCATTGAAAGAGAGGCCAACATTCGACGAGAGCAACTCAAGGATATTCTTACAAAGCAGGCTGAGTTGGGAGTCGAAGTTGCCGAAGTACCATCATACTACCTCGCAGATTCAAATCAAGGGCATGTAAAAGAAGATAATGAATCATTGACCAAGAAGGGGAGACTAGCAAACAAATTTGGTAAGAGAGAAAAATATGATAGAAGGGATCGGTTTTCGAAGAGGCATAGATCACATCAAAAGGATTCGTCTAATAACCCTTCTTTCAACAAGAGGGAGCCAACGTTGCTGCAGAAGCTTCTTAGTGCAGAAATTAGGAGAGACAGAAGCCACCTCCTGCAGGTGTTTAGGTTCATGGTAACAAACTCTTTCTTCAAAGATTGTCCTGACAAACCTTTGAAATTTCCTACTGTAGCAGTTAAAGAAAGCACGGGTGAGGAAGAGGTGGCTGAAAAGTTGTCCTCACTTGCAGAGAAGGATGCTTTAAAGGGAAACGATAAATCCATGGTTGGAAGAGTTTATAACAATCATTCTGGAAATAACAACGATGTCGGGAATTATGATGATAGTGGTGATGAAGATGATGGTGGAGGAGAGGGAACAGAGACACCTGGGGAGGAAGAGGAAGACATCATAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko05164 map03015 map05164
Pfam Domains
Protein Families
NUFIP1

Protein Analysis

565

Amino Acids

63.31

Weight (kDa)

9.38

Isoelectric Point (pI)

54.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUFIP1 PF10453 307 - 347 2.6e-10 FMR1-interacting protein 1 (NUFIP1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1575
AarI CACCTGC 1 cut(s) 1384
Acc36I ACCTGC 1 cut(s) 1384
AccBSI CCGCTC 1 cut(s) 970
AccI GTMKAC 1 cut(s) 661
AciI CCGC 1 cut(s) 970
AclI AACGTT 1 cut(s) 1337
AclWI GGATC 2 cut(s) 547, 1269
AcoI YGGCCR 1 cut(s) 897
AcsI RAATTY 7 cut(s) 114, 397, 457, 721, 1045, 1229, 1454
AfaI GTAC 2 cut(s) 133, 1143
AfiI CCNNNNNNNGG 8 cut(s) 95, 224, 266, 424, 425, 674, 1212, 1393
AflIII ACRYGT 1 cut(s) 390
AgsI TTSAA 9 cut(s) 462, 696, 720, 909, 1061, 1167, 1321, 1429, 1453
AjnI CCWGG 4 cut(s) 367, 597, 912, 1663
AjuI GAANNNNNNNTTGG 2 cut(s) 771, 803
AluBI AGCT 4 cut(s) 44, 152, 635, 1351
AluI AGCT 4 cut(s) 44, 152, 635, 1351
Alw26I GTCTC 4 cut(s) 573, 1210, 1368, 1652
AlwI GGATC 2 cut(s) 547, 1269
Ama87I CYCGRG 1 cut(s) 555
AoxI GGCC 6 cut(s) 215, 235, 558, 625, 897, 1068
ApeKI GCWGC 2 cut(s) 334, 1342
ApoI RAATTY 7 cut(s) 114, 397, 457, 721, 1045, 1229, 1454
ArsI GACNNNNNNTTYG 2 cut(s) 643, 675
Asp700I GAANNNNTTC 1 cut(s) 691
AspS9I GGNCC 2 cut(s) 236, 558
AsuC2I CCSGG 2 cut(s) 252, 537
AsuHPI GGTGA 5 cut(s) 68, 732, 767, 1499, 1637
AsuII TTCGAA 1 cut(s) 1271
AvaI CYCGRG 1 cut(s) 555
BaeGI GKGCMC 1 cut(s) 852
BalI TGGCCA 1 cut(s) 899
BbsI GAAGAC 1 cut(s) 1686
BbvI GCAGC 2 cut(s) 346, 1329
BccI CCATC 5 cut(s) 213, 514, 1009, 1153, 1631
BcgI CGANNNNNNTGC 2 cut(s) 445, 479
BciT130I CCWGG 4 cut(s) 369, 599, 914, 1665
BcnI CCSGG 2 cut(s) 252, 537
BcoDI GTCTC 4 cut(s) 573, 1210, 1368, 1652
BfaI CTAG 2 cut(s) 1220, 1693
BfmI CTRYAG 3 cut(s) 1343, 1391, 1464
BfuAI ACCTGC 1 cut(s) 1384
BglII AGATCT 1 cut(s) 932
BisI GCNGC 2 cut(s) 335, 1343
BlsI GCNGC 2 cut(s) 336, 1344
BmcAI AGTACT 1 cut(s) 133
Bme1390I CCNGG 6 cut(s) 252, 369, 537, 599, 914, 1665
BmeT110I CYCGRG 1 cut(s) 555
BmgT120I GGNCC 2 cut(s) 236, 558
BmiI GGNNCC 3 cut(s) 66, 229, 1332
BmrFI CCNGG 6 cut(s) 252, 369, 537, 599, 914, 1665
BmsI GCATC 2 cut(s) 72, 1522
BpiI GAAGAC 1 cut(s) 1686
BplI GAGNNNNNCTC 2 cut(s) 1074, 1106
BpmI CTGGAG 1 cut(s) 651
Bpu14I TTCGAA 1 cut(s) 1271
BpuEI CTTGAG 2 cut(s) 345, 1076
BpuMI CCSGG 2 cut(s) 252, 537
BsaBI GATNNNNATC 1 cut(s) 1167
BsaJI CCNNGG 6 cut(s) 368, 554, 778, 913, 1557, 1664
BsaXI ACNNNNNCTCC 4 cut(s) 1116, 1146, 1322, 1352
Bsc4I CCNNNNNNNGG 8 cut(s) 95, 224, 266, 424, 425, 674, 1212, 1393
Bse1I ACTGG 2 cut(s) 119, 668
Bse8I GATNNNNATC 1 cut(s) 1167
BseBI CCWGG 4 cut(s) 369, 599, 914, 1665
BseDI CCNNGG 6 cut(s) 368, 554, 778, 913, 1557, 1664
BseGI GGATG 1 cut(s) 1537
BseJI GATNNNNATC 1 cut(s) 1167
BseLI CCNNNNNNNGG 8 cut(s) 95, 224, 266, 424, 425, 674, 1212, 1393
BseMII CTCAG 3 cut(s) 233, 812, 1107
BseNI ACTGG 2 cut(s) 119, 668
BseRI GAGGAG 2 cut(s) 63, 1659
BseSI GKGCMC 1 cut(s) 852
BseXI GCAGC 2 cut(s) 346, 1329
BsgI GTGCAG 2 cut(s) 353, 1380
BshFI GGCC 6 cut(s) 217, 237, 560, 627, 899, 1070
BsiHKCI CYCGRG 1 cut(s) 555
BsiSI CCGG 2 cut(s) 251, 536
BslFI GGGAC 2 cut(s) 563, 691
BslI CCNNNNNNNGG 8 cut(s) 95, 224, 266, 424, 425, 674, 1212, 1393
BsmAI GTCTC 4 cut(s) 573, 1210, 1368, 1652
BsmFI GGGAC 2 cut(s) 563, 691
BsmI GAATGC 1 cut(s) 610
BsnI GGCC 6 cut(s) 217, 237, 560, 627, 899, 1070
BsoBI CYCGRG 1 cut(s) 555
Bsp119I TTCGAA 1 cut(s) 1271
Bsp1286I GDGCHC 1 cut(s) 852
Bsp143I GATC 4 cut(s) 539, 932, 1261, 1283
Bsp19I CCATGG 1 cut(s) 1557
BspACI CCGC 1 cut(s) 970
BspANI GGCC 6 cut(s) 217, 237, 560, 627, 899, 1070
BspCNI CTCAG 3 cut(s) 232, 811, 1108
BspHI TCATGA 1 cut(s) 165
BspLI GGNNCC 3 cut(s) 66, 229, 1332
BspMAI CTGCAG 2 cut(s) 1347, 1395
BspMI ACCTGC 1 cut(s) 1384
BspPI GGATC 2 cut(s) 547, 1269
BspT104I TTCGAA 1 cut(s) 1271
BsrBI CCGCTC 1 cut(s) 970
BsrI ACTGG 2 cut(s) 119, 668
BssECI CCNNGG 6 cut(s) 368, 554, 778, 913, 1557, 1664
BssMI GATC 4 cut(s) 539, 932, 1261, 1283
BssT1I CCWWGG 2 cut(s) 778, 1557
Bst2UI CCWGG 4 cut(s) 369, 599, 914, 1665
Bst4CI ACNGT 3 cut(s) 435, 701, 1465
Bst6I CTCTTC 6 cut(s) 910, 923, 1268, 1488, 1561, 1668
BstBI TTCGAA 1 cut(s) 1271
BstC8I GCNNGC 5 cut(s) 625, 629, 633, 920, 1113
BstDEI CTNAG 4 cut(s) 219, 798, 1116, 1355
BstDSI CCRYGG 1 cut(s) 1557
BstENI CCTNNNNNAGG 1 cut(s) 1391
BstF5I GGATG 1 cut(s) 1537
BstKTI GATC 4 cut(s) 542, 935, 1264, 1286
BstMAI GTCTC 4 cut(s) 573, 1210, 1368, 1652
BstMBI GATC 4 cut(s) 539, 932, 1261, 1283
BstMWI GCNNNNNNNGC 2 cut(s) 1339, 1348
BstNI CCWGG 4 cut(s) 369, 599, 914, 1665
BstNSI RCATGY 2 cut(s) 394, 1181
BstSCI CCNGG 6 cut(s) 250, 367, 535, 597, 912, 1663
BstSFI CTRYAG 3 cut(s) 1343, 1391, 1464
BstSLI GKGCMC 1 cut(s) 852
BstV1I GCAGC 2 cut(s) 346, 1329
BstV2I GAAGAC 1 cut(s) 1686
BstX2I RGATCY 1 cut(s) 932
BstYI RGATCY 1 cut(s) 932
BsuRI GGCC 6 cut(s) 217, 237, 560, 627, 899, 1070
BtgI CCRYGG 1 cut(s) 1557
BtgZI GCGATG 1 cut(s) 492
BtsCI GGATG 1 cut(s) 1537
BtsIMutI CAGTG 1 cut(s) 675
BveI ACCTGC 1 cut(s) 1384
Cac8I GCNNGC 5 cut(s) 625, 629, 633, 920, 1113
CciI TCATGA 1 cut(s) 165
Cfr13I GGNCC 2 cut(s) 236, 558
Csp6I GTAC 2 cut(s) 132, 1142
CviAII CATG 7 cut(s) 145, 166, 391, 472, 1178, 1408, 1558
CviQI GTAC 2 cut(s) 132, 1142
DdeI CTNAG 4 cut(s) 219, 798, 1116, 1355
DpnI GATC 4 cut(s) 541, 934, 1263, 1285
DpnII GATC 4 cut(s) 539, 932, 1261, 1283
DraI TTTAAA 1 cut(s) 1539
EaeI YGGCCR 1 cut(s) 897
Eam1104I CTCTTC 6 cut(s) 910, 923, 1268, 1488, 1561, 1668
EarI CTCTTC 6 cut(s) 910, 923, 1268, 1488, 1561, 1668
Eco130I CCWWGG 2 cut(s) 778, 1557
Eco147I AGGCCT 1 cut(s) 217
Eco88I CYCGRG 1 cut(s) 555
EcoNI CCTNNNNNAGG 1 cut(s) 1391
EcoRI GAATTC 1 cut(s) 457
EcoRII CCWGG 4 cut(s) 367, 597, 912, 1663
EcoT14I CCWWGG 2 cut(s) 778, 1557
ErhI CCWWGG 2 cut(s) 778, 1557
FaeI CATG 7 cut(s) 148, 169, 394, 475, 1181, 1411, 1561
FaqI GGGAC 2 cut(s) 563, 691
FatI CATG 7 cut(s) 144, 165, 390, 471, 1177, 1407, 1557
FauNDI CATATG 1 cut(s) 810
FblI GTMKAC 1 cut(s) 661
Fnu4HI GCNGC 2 cut(s) 335, 1343
FokI GGATG 1 cut(s) 1544
Fsp4HI GCNGC 2 cut(s) 335, 1343
FspBI CTAG 2 cut(s) 1220, 1693
GluI GCNGC 2 cut(s) 335, 1343
GsuI CTGGAG 1 cut(s) 651
HaeIII GGCC 6 cut(s) 217, 237, 560, 627, 899, 1070
HapII CCGG 2 cut(s) 251, 536
Hin1II CATG 7 cut(s) 148, 169, 394, 475, 1181, 1411, 1561
HindIII AAGCTT 1 cut(s) 1349
HpaII CCGG 2 cut(s) 251, 536
HphI GGTGA 5 cut(s) 68, 732, 767, 1499, 1637
Hpy166II GTNNAC 3 cut(s) 431, 662, 834
Hpy188I TCNGA 3 cut(s) 768, 792, 1051
Hpy188III TCNNGA 5 cut(s) 103, 166, 1439, 1587, 1606
Hpy8I GTNNAC 3 cut(s) 431, 662, 834
Hpy99I CGWCG 2 cut(s) 55, 1083
HpyAV CCTTC 5 cut(s) 875, 1204, 1251, 1323, 1522
HpyCH4III ACNGT 3 cut(s) 435, 701, 1465
HpyCH4IV ACGT 2 cut(s) 985, 1337
HpyF10VI GCNNNNNNNGC 2 cut(s) 1339, 1348
HpyF3I CTNAG 4 cut(s) 219, 798, 1116, 1355
HpySE526I ACGT 2 cut(s) 985, 1337
Hsp92II CATG 7 cut(s) 148, 169, 394, 475, 1181, 1411, 1561
Kzo9I GATC 4 cut(s) 539, 932, 1261, 1283
LmnI GCTCC 2 cut(s) 70, 1330
Lsp1109I GCAGC 2 cut(s) 346, 1329
LweI GCATC 2 cut(s) 72, 1522
MaeI CTAG 2 cut(s) 1220, 1693
MaeII ACGT 2 cut(s) 985, 1337
MaeIII GTNAC 5 cut(s) 3, 287, 710, 801, 1411
MalI GATC 4 cut(s) 541, 934, 1263, 1285
MbiI CCGCTC 1 cut(s) 970
MboI GATC 4 cut(s) 539, 932, 1261, 1283
MfeI CAATTG 3 cut(s) 92, 347, 727
MflI RGATCY 1 cut(s) 932
MhlI GDGCHC 1 cut(s) 852
MlsI TGGCCA 1 cut(s) 899
MluNI TGGCCA 1 cut(s) 899
MlyI GAGTC 1 cut(s) 1134
MmeI TCCRAC 3 cut(s) 60, 1035, 1543
Mox20I TGGCCA 1 cut(s) 899
MroXI GAANNNNTTC 1 cut(s) 691
MscI TGGCCA 1 cut(s) 899
MseI TTAA 4 cut(s) 395, 866, 1473, 1538
Msp20I TGGCCA 1 cut(s) 899
MspI CCGG 2 cut(s) 251, 536
MspR9I CCNGG 6 cut(s) 252, 369, 537, 599, 914, 1665
MunI CAATTG 3 cut(s) 92, 347, 727
Mva1269I GAATGC 1 cut(s) 610
MvaI CCWGG 4 cut(s) 369, 599, 914, 1665
MwoI GCNNNNNNNGC 2 cut(s) 1339, 1348
NciI CCSGG 2 cut(s) 252, 537
NcoI CCATGG 1 cut(s) 1557
NdeI CATATG 1 cut(s) 810
NdeII GATC 4 cut(s) 539, 932, 1261, 1283
NlaIII CATG 7 cut(s) 148, 169, 394, 475, 1181, 1411, 1561
NlaIV GGNNCC 3 cut(s) 66, 229, 1332
NmeAIII GCCGAG 1 cut(s) 41
NmuCI GTSAC 1 cut(s) 801
NspI RCATGY 2 cut(s) 394, 1181
NspV TTCGAA 1 cut(s) 1271
PagI TCATGA 1 cut(s) 165
PaqCI CACCTGC 1 cut(s) 1384
PceI AGGCCT 1 cut(s) 217
PciI ACATGT 1 cut(s) 390
PcsI WCGNNNNNNNCGW 1 cut(s) 1134
PctI GAATGC 1 cut(s) 610
PdmI GAANNNNTTC 1 cut(s) 691
PfoI TCCNGGA 1 cut(s) 535
PkrI GCNGC 2 cut(s) 336, 1344
PleI GAGTC 1 cut(s) 1133
PpsI GAGTC 1 cut(s) 1133
PscI ACATGT 1 cut(s) 390
PsiI TTATAA 1 cut(s) 1575
Psp1406I AACGTT 1 cut(s) 1337
Psp6I CCWGG 4 cut(s) 367, 597, 912, 1663
PspGI CCWGG 4 cut(s) 367, 597, 912, 1663
PspN4I GGNNCC 3 cut(s) 66, 229, 1332
PspPI GGNCC 2 cut(s) 236, 558
PstI CTGCAG 2 cut(s) 1347, 1395
PsuI RGATCY 1 cut(s) 932
RsaI GTAC 2 cut(s) 133, 1143
RsaNI GTAC 2 cut(s) 132, 1142
SaqAI TTAA 4 cut(s) 395, 866, 1473, 1538
SatI GCNGC 2 cut(s) 335, 1343
Sau3AI GATC 4 cut(s) 539, 932, 1261, 1283
Sau96I GGNCC 2 cut(s) 236, 558
SbfI CCTGCAGG 1 cut(s) 1395
ScaI AGTACT 1 cut(s) 133
SchI GAGTC 1 cut(s) 1134
ScrFI CCNGG 6 cut(s) 252, 369, 537, 599, 914, 1665
SdaI CCTGCAGG 1 cut(s) 1395
SduI GDGCHC 1 cut(s) 852
SfaNI GCATC 2 cut(s) 72, 1522
SfcI CTRYAG 3 cut(s) 1343, 1391, 1464
SfuI TTCGAA 1 cut(s) 1271
SmlI CTYRAG 2 cut(s) 360, 1091
SmoI CTYRAG 2 cut(s) 360, 1091
Sse8387I CCTGCAGG 1 cut(s) 1395
SseBI AGGCCT 1 cut(s) 217
SsiI CCGC 1 cut(s) 970
SspMI CTAG 2 cut(s) 1220, 1693
StuI AGGCCT 1 cut(s) 217
StyD4I CCNGG 6 cut(s) 250, 367, 535, 597, 912, 1663
StyI CCWWGG 2 cut(s) 778, 1557
TaaI ACNGT 3 cut(s) 435, 701, 1465
TaiI ACGT 2 cut(s) 988, 1340
TaqI TCGA 3 cut(s) 1078, 1128, 1271
TatI WGTACW 1 cut(s) 131
Tru1I TTAA 4 cut(s) 395, 866, 1473, 1538
Tru9I TTAA 4 cut(s) 395, 866, 1473, 1538
TscAI CASTG 1 cut(s) 675
TseFI GTSAC 1 cut(s) 801
TseI GCWGC 2 cut(s) 334, 1342
Tsp45I GTSAC 1 cut(s) 801
TspDTI ATGAA 9 cut(s) 154, 182, 317, 428, 488, 827, 1209, 1396, 1644
TspGWI ACGGA 2 cut(s) 220, 506
TspRI CASTG 1 cut(s) 675
XagI CCTNNNNNAGG 1 cut(s) 1391
XapI RAATTY 7 cut(s) 114, 397, 457, 721, 1045, 1229, 1454
XceI RCATGY 2 cut(s) 394, 1181
XmiI GTMKAC 1 cut(s) 661
XmnI GAANNNNTTC 1 cut(s) 691
XspI CTAG 2 cut(s) 1220, 1693
ZrmI AGTACT 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.