Rorug03G0244300

Nuclear fragile X mental retardation-interacting protein 1 (NUFIP1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005893
Physical Location & Seq
Forward (+)
22816591 .. 22822117
5527 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0244300.1

Sequence Viewer

Length: 2223 bp
ATGGCGAAGCTGAACCGGTTCGTGTACTTCATGGCGGTTTTCTTCGCGGCGGTTCTGGTGGCTTCCCCGGCTGGGGCTTTTACTGATCCACTTGACGTCGTAGCTCTTCATGATCTTTACATGACCCTCAACCAACCGCCACAGCTGACGGGTTGGAGATTGAATGGTGGGGATCCTTGCGATGAATCGTGGAATGGAGTGTCATGCTTTGGCTCTTCTGTGATATACCTTAAACTTAATGGTCTAAACCTCTCTGGGTATATTACTGCCGAGATCTATAATCTCTACAGTTTGAAGCAACTGGATATTAGTTCCAATACCATTGGTGGTGAAATTCCATATGTCTTACCCCCCAATGCGACTCATATAAATATGGCATACAACTATTTGATCCAAAATATTCCCCCATCCTTACCTGGCATGAAAAGTCTCCGACATTTGAATCTAAGCCACAATTTGTTATCTGGACCCATTGGCAATGTTTTTACAGGCCTACAGAATTTAAGAGAAATGGATTTGTCATATAATAACTTTACTGGAGATCTGCCAAGTTCATTTGGATCCCTCACAAATCTCACTGGACTGTACTTGCAGAAGAACCAATTCACTGGATCAGTTGCTTACCTAGCCGAACTTCCACTAATTGATCTGAACATCCAAGATAATTATTTCAGTGGTATCATCCCCAGTCATTTTCAGTCCATACCAAATTTATGGATTGGGGGAAATAGTTTTCATCGTCCAGGGGACAACTATCCACCCTGGGATTTCCCTCTGGAAACGCAGACAAGTGTCATTGAACAGAACATCCACCCCCCTCCAGCAACTCAGTCAAGTGCCATGGAGAAGAGCAAGCCCTCTACCAAATTAGGTGGACACAAGAAGAGGAGGCTTGGCCCTGGAGGAATAGCTTTTATGATTGGTGGAGGAACTCTAGTCGCCTCATGTGTGGCACTTTACATTGCAGTTCGTATCAAACAATCACGTGCACAGAAGCTTATAACTTTGGGGGGCAGTAATAGTTCAATCCACTCTTTCTCAGTCATAAATACGGCCAAAGATAGTCCTATGAATGCACGAGAAGAAAGCCCACCAATACTGACTTTCAGGCCACCCATTCTACTTCCAAGGCGACTACCTCCAGTTTATCATAGCAGAGTAGAGAAAACTAGAAGAAAAAGTTTCTCTGATAAATGCAGTTTCCCAGTGAGAATCAAACTATATACTGTGGCAGAGCTTCAGTCAGCTACGAACAGCTTTGCGGAAGAAAAGTTTCTGGGCCAAGGATCTCTTGGTTCTGTTTACAAAGCCGCGTTTCCTGATGGCCAAATTTTGGCTGTAAAGAACATCAAGATGTCTGGACTCTCTTTCCATGAAGAAGAACAATTCCTGGATGTCGTTTGGACTGTTTCGCGTTTGAAGCACCCAAACATTGTACCACTTGTTGGCTATTGTGTGGAGAATGGACAACATCTCCTTGTGTATGAATATGTTGGAAATTTATCCCTTGATGAGGCACTTCACAGCGATGCATACAAGCCTCTGTCCTGGGGCCTCCGTCTCCAAATTGCTCTTGGTGTTGCTCAAGCTTTGGACTATCTGCACTCCACATTGTCGCCTTCAGTAGCTCATGGCAACTTGAAGAGCGAAAATATCTTACTTGATGAAGAACTTGCTCCTCATATCTGTGACTGCGGACTTGCTATTTTGAGGCCACTTACAAGCAATAGTCTTAAACTGAAGGCTTCAGAAAATGCTGTTGGTGACACTGGCTACATTGCACCTGAGCATGGCCTACCAGGGTTTGATAAGACAAAGAGTGACATCTATGCCTTCGGAGTGCTGCTTTTGGAGCTCTTAACAGGAAGGAGACCTTCTGATAGTTCAAGACCAAGGGAAGAGCAATCACTGGTGAAATGGGCTTCATTGCGGCTTCATGACACTGAGAGTCTGGATGAGATGATCGATCCAGGCATTAAAAAGACATGTTCCTCCAAGGCTCTCTCAAATTTTGCTGATATTATCTCCCTCTGTATACAGCCAGTGAAGGAGTTCCGACCACCAATGTCTGAAGTTGTGGGATCTCTAACACAACTAATTCAGAAGCTCAAGGGGAAAGGCAATGGAGAGGATGGTACACAAGTTGACCCCTTCGAGAGATCTTTTCGTTCAACCAACACCCGCTTCATGGGCTCACCTACATTGAGCTATTTTTCCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko05164 map03015 map05164
Pfam Domains
Protein Families
NUFIP1

Protein Analysis

740

Amino Acids

81.32

Weight (kDa)

7.29

Isoelectric Point (pI)

47.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 32 - 69 8.3e-06 Leucine rich repeat N-terminal domain
LRR_8 PF13855 161 - 202 2.8e-07 Leucine rich repeat
Pkinase PF00069 421 - 693 1.5e-33 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 423 - 695 6.1e-34 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1001
AatII GACGTC 1 cut(s) 99
AccB7I CCANNNNNTGG 2 cut(s) 1333, 1445
AccI GTMKAC 1 cut(s) 2035
AccII CGCG 3 cut(s) 47, 1313, 1414
AciI CCGC 9 cut(s) 35, 47, 50, 137, 1262, 1311, 1695, 1930, 2182
AcoI YGGCCR 2 cut(s) 1053, 1324
AcsI RAATTY 6 cut(s) 333, 499, 709, 1329, 1498, 2008
AcuI CTGAAG 5 cut(s) 1223, 1605, 1731, 1760, 2091
AcvI CACGTG 1 cut(s) 986
AcyI GRCGYC 1 cut(s) 96
AfaI GTAC 4 cut(s) 26, 587, 1437, 2137
AfiI CCNNNNNNNGG 7 cut(s) 72, 73, 1333, 1445, 1513, 1790, 2188
AflIII ACRYGT 1 cut(s) 1985
AgeI ACCGGT 1 cut(s) 15
AgsI TTSAA 9 cut(s) 163, 295, 442, 800, 1026, 1420, 1642, 1887, 2172
AjnI CCWGG 8 cut(s) 415, 742, 761, 898, 1389, 1547, 1798, 1969
AjuI GAANNNNNNNTTGG 4 cut(s) 1743, 1775, 2168, 2200
AloI GAACNNNNNNTCC 2 cut(s) 792, 824
Alw21I GWGCWC 2 cut(s) 991, 1857
Alw26I GTCTC 3 cut(s) 434, 1565, 1864
Alw44I GTGCAC 1 cut(s) 987
AoxI GGCC 9 cut(s) 490, 895, 1053, 1109, 1279, 1324, 1552, 1712, 1792
ApaLI GTGCAC 1 cut(s) 987
ApeKI GCWGC 1 cut(s) 1843
ApoI RAATTY 6 cut(s) 333, 499, 709, 1329, 1498, 2008
AsiGI ACCGGT 1 cut(s) 15
Asp700I GAANNNNTTC 4 cut(s) 17, 602, 1272, 2051
AspS9I GGNCC 4 cut(s) 467, 896, 1279, 1552
AsuC2I CCSGG 1 cut(s) 68
AsuHPI GGTGA 4 cut(s) 341, 1775, 1924, 2187
AvaII GGWCC 1 cut(s) 467
BaeGI GKGCMC 1 cut(s) 991
BaeI ACNNNNGTAYC 2 cut(s) 2127, 2160
BalI TGGCCA 1 cut(s) 1326
BamHI GGATCC 2 cut(s) 172, 560
BanII GRGCYC 2 cut(s) 1857, 2195
BauI CACGAG 1 cut(s) 1077
BbrPI CACGTG 1 cut(s) 986
Bbv12I GWGCWC 2 cut(s) 991, 1857
BbvI GCAGC 1 cut(s) 1830
BccI CCATC 3 cut(s) 415, 1316, 2126
BceAI ACGGC 1 cut(s) 1068
BciT130I CCWGG 8 cut(s) 417, 744, 763, 900, 1391, 1549, 1800, 1971
BcnI CCSGG 1 cut(s) 68
BcoDI GTCTC 3 cut(s) 434, 1565, 1864
BfaI CTAG 3 cut(s) 626, 935, 1170
BfmI CTRYAG 2 cut(s) 286, 494
BglII AGATCT 3 cut(s) 273, 541, 2159
BisI GCNGC 4 cut(s) 48, 1311, 1844, 1931
BlsI GCNGC 4 cut(s) 49, 1312, 1845, 1932
Bme1390I CCNGG 9 cut(s) 68, 417, 744, 763, 900, 1391, 1549, 1800, 1971
Bme18I GGWCC 1 cut(s) 467
BmgT120I GGNCC 4 cut(s) 467, 896, 1279, 1552
BmiI GGNNCC 4 cut(s) 174, 469, 562, 1553
BmrFI CCNGG 9 cut(s) 68, 417, 744, 763, 900, 1391, 1549, 1800, 1971
BmrI ACTGGG 2 cut(s) 681, 1199
BmsI GCATC 1 cut(s) 1519
BmuI ACTGGG 2 cut(s) 681, 1199
BoxI GACNNNNGTC 1 cut(s) 791
BpmI CTGGAG 4 cut(s) 558, 804, 921, 1125
Bpu10I CCTNAGC 1 cut(s) 1785
BpuEI CTTGAG 2 cut(s) 1569, 2093
BpuMI CCSGG 1 cut(s) 68
Bsa29I ATCGAT 1 cut(s) 1965
BsaAI YACGTR 1 cut(s) 986
BsaHI GRCGYC 1 cut(s) 96
BsaI GGTCTC 1 cut(s) 1864
BsaWI WCCGGW 1 cut(s) 15
BsaXI ACNNNNNCTCC 2 cut(s) 1458, 1488
Bsc4I CCNNNNNNNGG 7 cut(s) 72, 73, 1333, 1445, 1513, 1790, 2188
Bse118I RCCGGY 1 cut(s) 15
Bse3DI GCAATG 5 cut(s) 484, 960, 1776, 1925, 2128
BseBI CCWGG 8 cut(s) 417, 744, 763, 900, 1391, 1549, 1800, 1971
BseCI ATCGAT 1 cut(s) 1965
BseGI GGATG 7 cut(s) 407, 654, 681, 807, 1399, 1960, 2137
BseLI CCNNNNNNNGG 7 cut(s) 72, 73, 1333, 1445, 1513, 1790, 2188
BseMI GCAATG 5 cut(s) 484, 960, 1776, 1925, 2128
BseMII CTCAG 4 cut(s) 842, 1053, 1776, 1935
BseRI GAGGAG 2 cut(s) 901, 1668
BseSI GKGCMC 1 cut(s) 991
BseXI GCAGC 1 cut(s) 1830
BseYI CCCAGC 1 cut(s) 71
BsgI GTGCAG 1 cut(s) 1586
Bsh1236I CGCG 3 cut(s) 47, 1313, 1414
BshFI GGCC 9 cut(s) 492, 897, 1055, 1111, 1281, 1326, 1554, 1714, 1794
BshTI ACCGGT 1 cut(s) 15
BshVI ATCGAT 1 cut(s) 1965
BsiHKAI GWGCWC 2 cut(s) 991, 1857
BsiSI CCGG 2 cut(s) 16, 68
BslFI GGGAC 1 cut(s) 761
BslI CCNNNNNNNGG 7 cut(s) 72, 73, 1333, 1445, 1513, 1790, 2188
BsmAI GTCTC 3 cut(s) 434, 1565, 1864
BsmBI CGTCTC 1 cut(s) 1565
BsmFI GGGAC 1 cut(s) 761
BsmI GAATGC 1 cut(s) 1078
BsnI GGCC 9 cut(s) 492, 897, 1055, 1111, 1281, 1326, 1554, 1714, 1794
Bso31I GGTCTC 1 cut(s) 1864
Bsp1286I GDGCHC 3 cut(s) 991, 1857, 2195
Bsp19I CCATGG 1 cut(s) 840
BspACI CCGC 9 cut(s) 35, 47, 50, 137, 1262, 1311, 1695, 1930, 2182
BspANI GGCC 9 cut(s) 492, 897, 1055, 1111, 1281, 1326, 1554, 1714, 1794
BspCNI CTCAG 4 cut(s) 841, 1052, 1777, 1936
BspDI ATCGAT 1 cut(s) 1965
BspFNI CGCG 3 cut(s) 47, 1313, 1414
BspHI TCATGA 2 cut(s) 109, 1936
BspLI GGNNCC 4 cut(s) 174, 469, 562, 1553
BspQI GCTCTTC 5 cut(s) 111, 220, 842, 1637, 1893
BspTNI GGTCTC 1 cut(s) 1864
BsrDI GCAATG 5 cut(s) 484, 960, 1776, 1925, 2128
BsrFI RCCGGY 1 cut(s) 15
BssAI RCCGGY 1 cut(s) 15
BssNAI GTATAC 1 cut(s) 2036
BssNI GRCGYC 1 cut(s) 96
BssSI CACGAG 1 cut(s) 1077
BssT1I CCWWGG 5 cut(s) 840, 1127, 1282, 1892, 1995
Bst1107I GTATAC 1 cut(s) 2036
Bst2BI CACGAG 1 cut(s) 1077
Bst2UI CCWGG 8 cut(s) 417, 744, 763, 900, 1391, 1549, 1800, 1971
Bst4CI ACNGT 4 cut(s) 290, 585, 1228, 1408
Bst6I CTCTTC 6 cut(s) 111, 220, 842, 878, 1637, 1893
BstACI GRCGYC 1 cut(s) 96
BstBAI YACGTR 1 cut(s) 986
BstC8I GCNNGC 1 cut(s) 854
BstDEI CTNAG 5 cut(s) 446, 828, 1039, 1785, 1944
BstDSI CCRYGG 1 cut(s) 840
BstENI CCTNNNNNAGG 1 cut(s) 1511
BstF5I GGATG 7 cut(s) 407, 654, 681, 807, 1399, 1960, 2137
BstFNI CGCG 3 cut(s) 47, 1313, 1414
BstMAI GTCTC 3 cut(s) 434, 1565, 1864
BstMWI GCNNNNNNNGC 5 cut(s) 68, 626, 1420, 1852, 2190
BstNI CCWGG 8 cut(s) 417, 744, 763, 900, 1391, 1549, 1800, 1971
BstNSI RCATGY 1 cut(s) 1989
BstPAI GACNNNNGTC 1 cut(s) 791
BstSCI CCNGG 9 cut(s) 66, 415, 742, 761, 898, 1389, 1547, 1798, 1969
BstSFI CTRYAG 2 cut(s) 286, 494
BstSLI GKGCMC 1 cut(s) 991
BstUI CGCG 3 cut(s) 47, 1313, 1414
BstV1I GCAGC 1 cut(s) 1830
BstX2I RGATCY 7 cut(s) 172, 273, 541, 560, 1286, 2081, 2159
BstXI CCANNNNNNTGG 2 cut(s) 608, 714
BstYI RGATCY 7 cut(s) 172, 273, 541, 560, 1286, 2081, 2159
BstZ17I GTATAC 1 cut(s) 2036
Bsu15I ATCGAT 1 cut(s) 1965
BsuRI GGCC 9 cut(s) 492, 897, 1055, 1111, 1281, 1326, 1554, 1714, 1794
BsuTUI ATCGAT 1 cut(s) 1965
BtgI CCRYGG 1 cut(s) 840
BtgZI GCGATG 2 cut(s) 195, 1542
BtsCI GGATG 7 cut(s) 407, 654, 681, 807, 1399, 1960, 2137
BtsIMutI CAGTG 8 cut(s) 576, 606, 679, 1212, 1767, 1907, 1941, 2049
Cac8I GCNNGC 1 cut(s) 854
CciI TCATGA 2 cut(s) 109, 1936
Cfr10I RCCGGY 1 cut(s) 15
Cfr13I GGNCC 4 cut(s) 467, 896, 1279, 1552
ClaI ATCGAT 1 cut(s) 1965
Csp6I GTAC 4 cut(s) 25, 586, 1436, 2136
CspAI ACCGGT 1 cut(s) 15
CspCI CAANNNNNGTGG 2 cut(s) 853, 888
CviQI GTAC 4 cut(s) 25, 586, 1436, 2136
DdeI CTNAG 5 cut(s) 446, 828, 1039, 1785, 1944
EaeI YGGCCR 2 cut(s) 1053, 1324
Eam1104I CTCTTC 6 cut(s) 111, 220, 842, 878, 1637, 1893
EarI CTCTTC 6 cut(s) 111, 220, 842, 878, 1637, 1893
Ecl136II GAGCTC 1 cut(s) 1855
Eco130I CCWWGG 5 cut(s) 840, 1127, 1282, 1892, 1995
Eco147I AGGCCT 1 cut(s) 492
Eco24I GRGCYC 2 cut(s) 1857, 2195
Eco31I GGTCTC 1 cut(s) 1864
Eco47I GGWCC 1 cut(s) 467
Eco53kI GAGCTC 1 cut(s) 1855
Eco57I CTGAAG 5 cut(s) 1223, 1605, 1731, 1760, 2091
Eco72I CACGTG 1 cut(s) 986
EcoICRI GAGCTC 1 cut(s) 1855
EcoNI CCTNNNNNAGG 1 cut(s) 1511
EcoO109I RGGNCCY 1 cut(s) 1552
EcoRII CCWGG 8 cut(s) 415, 742, 761, 898, 1389, 1547, 1798, 1969
EcoT14I CCWWGG 5 cut(s) 840, 1127, 1282, 1892, 1995
EcoT22I ATGCAT 1 cut(s) 1534
EcoT38I GRGCYC 2 cut(s) 1857, 2195
ErhI CCWWGG 5 cut(s) 840, 1127, 1282, 1892, 1995
Esp3I CGTCTC 1 cut(s) 1565
FalI AAGNNNNNCTT 4 cut(s) 1275, 1307, 1858, 1890
FaqI GGGAC 1 cut(s) 761
FauI CCCGC 1 cut(s) 2189
FauNDI CATATG 1 cut(s) 340
FblI GTMKAC 1 cut(s) 2035
Fnu4HI GCNGC 4 cut(s) 48, 1311, 1844, 1931
FokI GGATG 7 cut(s) 394, 641, 668, 794, 1406, 1967, 2144
FriOI GRGCYC 2 cut(s) 1857, 2195
Fsp4HI GCNGC 4 cut(s) 48, 1311, 1844, 1931
FspBI CTAG 3 cut(s) 626, 935, 1170
GluI GCNGC 4 cut(s) 48, 1311, 1844, 1931
GsaI CCCAGC 1 cut(s) 75
GsuI CTGGAG 4 cut(s) 558, 804, 921, 1125
HaeIII GGCC 9 cut(s) 492, 897, 1055, 1111, 1281, 1326, 1554, 1714, 1794
HapII CCGG 2 cut(s) 16, 68
Hin1I GRCGYC 1 cut(s) 96
HincII GTYRAC 1 cut(s) 2146
HindII GTYRAC 1 cut(s) 2146
HindIII AAGCTT 2 cut(s) 995, 1587
HinfI GANTC 6 cut(s) 185, 361, 442, 1212, 1362, 1948
HpaII CCGG 2 cut(s) 16, 68
HphI GGTGA 4 cut(s) 341, 1775, 1924, 2187
Hpy166II GTNNAC 7 cut(s) 25, 875, 989, 1303, 2036, 2138, 2146
Hpy188I TCNGA 9 cut(s) 434, 651, 1189, 1750, 1838, 1879, 2057, 2071, 2103
Hpy8I GTNNAC 7 cut(s) 25, 875, 989, 1303, 2036, 2138, 2146
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 7 cut(s) 1629, 1735, 1843, 1860, 1884, 2041, 2161
HpyCH4III ACNGT 4 cut(s) 290, 585, 1228, 1408
HpyCH4IV ACGT 2 cut(s) 96, 985
HpyCH4V TGCA 8 cut(s) 592, 965, 989, 1076, 1197, 1532, 1603, 1781
HpyF10VI GCNNNNNNNGC 5 cut(s) 68, 626, 1420, 1852, 2190
HpyF3I CTNAG 5 cut(s) 446, 828, 1039, 1785, 1944
HpySE526I ACGT 2 cut(s) 96, 985
Hsp92I GRCGYC 1 cut(s) 96
LguI GCTCTTC 5 cut(s) 111, 220, 842, 1637, 1893
LmnI GCTCC 2 cut(s) 1681, 1852
Lsp1109I GCAGC 1 cut(s) 1830
LweI GCATC 1 cut(s) 1519
MaeI CTAG 3 cut(s) 626, 935, 1170
MaeII ACGT 2 cut(s) 96, 985
MaeIII GTNAC 3 cut(s) 1688, 1763, 1820
MflI RGATCY 7 cut(s) 172, 273, 541, 560, 1286, 2081, 2159
MhlI GDGCHC 3 cut(s) 991, 1857, 2195
MlsI TGGCCA 1 cut(s) 1326
MluNI TGGCCA 1 cut(s) 1326
MlyI GAGTC 3 cut(s) 355, 1356, 1957
MmeI TCCRAC 4 cut(s) 134, 457, 1474, 2080
Mox20I TGGCCA 1 cut(s) 1326
Mph1103I ATGCAT 1 cut(s) 1534
MroXI GAANNNNTTC 4 cut(s) 17, 602, 1272, 2051
MscI TGGCCA 1 cut(s) 1326
MseI TTAA 6 cut(s) 231, 237, 503, 1734, 1859, 1977
MslI CAYNNNNRTG 3 cut(s) 1352, 1527, 1686
Msp20I TGGCCA 1 cut(s) 1326
MspA1I CMGCKG 1 cut(s) 145
MspI CCGG 2 cut(s) 16, 68
MspR9I CCNGG 9 cut(s) 68, 417, 744, 763, 900, 1391, 1549, 1800, 1971
Mva1269I GAATGC 1 cut(s) 1078
MvaI CCWGG 8 cut(s) 417, 744, 763, 900, 1391, 1549, 1800, 1971
MvnI CGCG 3 cut(s) 47, 1313, 1414
MwoI GCNNNNNNNGC 5 cut(s) 68, 626, 1420, 1852, 2190
NciI CCSGG 1 cut(s) 68
NcoI CCATGG 1 cut(s) 840
NdeI CATATG 1 cut(s) 340
NlaIV GGNNCC 4 cut(s) 174, 469, 562, 1553
NmeAIII GCCGAG 1 cut(s) 295
NmuCI GTSAC 3 cut(s) 1688, 1763, 1820
NsiI ATGCAT 1 cut(s) 1534
NspI RCATGY 1 cut(s) 1989
PagI TCATGA 2 cut(s) 109, 1936
PasI CCCWGGG 1 cut(s) 762
PceI AGGCCT 1 cut(s) 492
PciI ACATGT 1 cut(s) 1985
PciSI GCTCTTC 5 cut(s) 111, 220, 842, 1637, 1893
PctI GAATGC 1 cut(s) 1078
PdmI GAANNNNTTC 4 cut(s) 17, 602, 1272, 2051
PfeI GAWTC 3 cut(s) 185, 442, 1212
PflMI CCANNNNNTGG 2 cut(s) 1333, 1445
PfoI TCCNGGA 1 cut(s) 1389
PinAI ACCGGT 1 cut(s) 15
PkrI GCNGC 4 cut(s) 49, 1312, 1845, 1932
PleI GAGTC 3 cut(s) 355, 1356, 1956
PmaCI CACGTG 1 cut(s) 986
PmlI CACGTG 1 cut(s) 986
PpsI GAGTC 3 cut(s) 355, 1356, 1956
Ppu21I YACGTR 1 cut(s) 986
PscI ACATGT 1 cut(s) 1985
PshAI GACNNNNGTC 1 cut(s) 791
PsiI TTATAA 1 cut(s) 1001
Psp124BI GAGCTC 1 cut(s) 1857
Psp6I CCWGG 8 cut(s) 415, 742, 761, 898, 1389, 1547, 1798, 1969
PspCI CACGTG 1 cut(s) 986
PspFI CCCAGC 1 cut(s) 71
PspGI CCWGG 8 cut(s) 415, 742, 761, 898, 1389, 1547, 1798, 1969
PspN4I GGNNCC 4 cut(s) 174, 469, 562, 1553
PspPI GGNCC 4 cut(s) 467, 896, 1279, 1552
PsuI RGATCY 7 cut(s) 172, 273, 541, 560, 1286, 2081, 2159
PvuII CAGCTG 1 cut(s) 145
RsaI GTAC 4 cut(s) 26, 587, 1437, 2137
RsaNI GTAC 4 cut(s) 25, 586, 1436, 2136
RseI CAYNNNNRTG 3 cut(s) 1352, 1527, 1686
SacI GAGCTC 1 cut(s) 1857
SapI GCTCTTC 5 cut(s) 111, 220, 842, 1637, 1893
SaqAI TTAA 6 cut(s) 231, 237, 503, 1734, 1859, 1977
SatI GCNGC 4 cut(s) 48, 1311, 1844, 1931
Sau96I GGNCC 4 cut(s) 467, 896, 1279, 1552
SchI GAGTC 3 cut(s) 355, 1356, 1957
ScrFI CCNGG 9 cut(s) 68, 417, 744, 763, 900, 1391, 1549, 1800, 1971
SduI GDGCHC 3 cut(s) 991, 1857, 2195
SfaNI GCATC 1 cut(s) 1519
SfcI CTRYAG 2 cut(s) 286, 494
SinI GGWCC 1 cut(s) 467
SmiMI CAYNNNNRTG 3 cut(s) 1352, 1527, 1686
SmlI CTYRAG 2 cut(s) 1584, 2108
SmoI CTYRAG 2 cut(s) 1584, 2108
SseBI AGGCCT 1 cut(s) 492
SsiI CCGC 9 cut(s) 35, 47, 50, 137, 1262, 1311, 1695, 1930, 2182
SspI AATATT 1 cut(s) 400
SspMI CTAG 3 cut(s) 626, 935, 1170
SstI GAGCTC 1 cut(s) 1857
StuI AGGCCT 1 cut(s) 492
StyD4I CCNGG 9 cut(s) 66, 415, 742, 761, 898, 1389, 1547, 1798, 1969
StyI CCWWGG 5 cut(s) 840, 1127, 1282, 1892, 1995
TaaI ACNGT 4 cut(s) 290, 585, 1228, 1408
TaiI ACGT 2 cut(s) 99, 988
TaqI TCGA 2 cut(s) 1965, 2154
TatI WGTACW 2 cut(s) 24, 585
TauI GCSGC 3 cut(s) 50, 1313, 1933
TfiI GAWTC 3 cut(s) 185, 442, 1212
Tru1I TTAA 6 cut(s) 231, 237, 503, 1734, 1859, 1977
Tru9I TTAA 6 cut(s) 231, 237, 503, 1734, 1859, 1977
TscAI CASTG 8 cut(s) 583, 613, 679, 1212, 1774, 1914, 1948, 2049
TseFI GTSAC 3 cut(s) 1688, 1763, 1820
TseI GCWGC 1 cut(s) 1843
Tsp45I GTSAC 3 cut(s) 1688, 1763, 1820
TspGWI ACGGA 1 cut(s) 1547
TspRI CASTG 8 cut(s) 583, 613, 679, 1212, 1774, 1914, 1948, 2049
Van91I CCANNNNNTGG 2 cut(s) 1333, 1445
VneI GTGCAC 1 cut(s) 987
VpaK11BI GGWCC 1 cut(s) 467
XagI CCTNNNNNAGG 1 cut(s) 1511
XapI RAATTY 6 cut(s) 333, 499, 709, 1329, 1498, 2008
XceI RCATGY 1 cut(s) 1989
XcmI CCANNNNNNNNNTGG 2 cut(s) 1289, 1571
XmiI GTMKAC 1 cut(s) 2035
XmnI GAANNNNTTC 4 cut(s) 17, 602, 1272, 2051
XspI CTAG 3 cut(s) 626, 935, 1170
ZraI GACGTC 1 cut(s) 97
Zsp2I ATGCAT 1 cut(s) 1534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.