Rh3AG294000

Nuclear fragile X mental retardation-interacting protein 1 (NUFIP1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0005893
Physical Location & Seq
Forward (+)
35616911 .. 35624327
7417 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG294000.1

Sequence Viewer

Length: 1698 bp
ATGTTACCTAACTATAATTCCTTTCACCAAAATTCCGGCCAAGCTCACCCGGCCGGCGGTAATGGTGGTAGCTCTGCTCCATCTCCTCAACAGCAGCAGGGTATGCCATTTAATCCATCACTTGCAAATTCATGTAATATGTTGCCACCTTCGATGGGACCTCAGCCTGGTTTCATGAATTTATCTAACAATCTTCTTCCGATGCAAAATAACCATATGCGTGTGCCCATGTCGCAATTTGGTTCCCTTGGTCCCATATCTCAACCAGGTTATCCTAGTGTTGGATTTTGTGGTCCACAGAATAATGCACTCAATATGAACTTGGCTGCTTCCTATCCATTTAACGGGCAGTTCTGCAATATGGGGCAGAATCTCAATCAAGTAAATCAAAATATGGGTTTTCTACCTGGTCAATTGTTTGGAAATAATCTGTTGAATTTACAGCAGATGAATCAAAATATTGGGCCATATGGGCAATTCTGTCCGCCAAATCTGTTGAATAGGAATCAGGTTGTGCCAATGCAAATGCAAATGCAAAGCCCTTGCCAAGGTGGTCCTAATTTTGCATTTGGGGGATCAAATCAAGCACCTCAGGCTGCAGTTCTGCAAAATCCTGCTTTTTTTGCAACTCCTAATTCTGGTAATGTGCACTCTCCAAATCAGGCCGGACAACAAATCAACCAGAATCAGCAAAACAACGTCTTCCGAGGGACACAGGGAAATCATATAAAAGCTGGTGGCGTTTACAATTCGAATTCTGACAGAAAAACTTCGCCAAGCAAAAATTTTATAAAGCATCCAAAACGAGGGTTGCAACCGCAAGGGGGATTTCAGAAGTCTCAGTTTCATCAAATCAAAAATGCAAAGGGAAAGTTTGCTTTTGCCAACAGGCAAAACAGAAAAGGGCTAAGCAATGAGAAGGAAGGAAAATCTGCTCTTCCCGACTCTATGAATCAAGGCAGGGAAAGGAAAAGATCTCTTTCTTTGTCCTATACGGAACAAGAAGTTCAACTGTGGCGTGAAGGACGTAGGAAGAACTACCCATCAAACACCAACATAGAGAAGAAGCTAAGTGAAAAGATGATAGACTCGGAGGCCATTGAGAGAGAGGCCAAAGCACGAAGGGAGCAACTCAAGGAGATTCTTACAAAGCAGGCTGAGATGGGAGTTGAAGTCGCTGAGATACCATCTCACTACCTCTCAGATTCAGCAAAACAAGGGAATGGAAGAGAGGAGAATAAGTCATGGACTAACAATCGGAGATCACAAAACAAGTTCGTTAAGAGAGACAGATATGATAAGAAGGACCGGTTTGCAAAGAGGCAAAGATCAGGTCAGAAGGATTCATCTAATGACCCTTCATTCAGCAAAAGGGAGCCAACCTTACTGCAGAAACTTCTTAGTTCAGATATCAAGAGAGATAAAAGTCGCTTATGCCAGGTGTTTAGGTTCATGGTGACGAACTCTTTCTTTAAAGATTTTCCTGACAAACCTTTGAAATTTCCTACTGTAGTAGTTAAAGAAAGTGTGGTGGGGGAAGGTGTGGTTGAAGATTTGTCTTCAGTTGCCGGGAAAGGTGCTTATGGGGGCCGTGACAAGTCCATGTTTGAAAAAGTTGGTAACAATGATTATAAAGATTGTTGTCGCAATGATGATTGTGTTGAAGATGAGGAAGAAGAGGGAGAGATCATCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko05164 map03015 map05164
Pfam Domains
Protein Families
NUFIP1

Protein Analysis

565

Amino Acids

62.98

Weight (kDa)

9.53

Isoelectric Point (pI)

49.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUFIP1 PF10453 328 - 360 1.1e-08 FMR1-interacting protein 1 (NUFIP1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 791, 1632
AciI CCGC 3 cut(s) 57, 485, 818
AclWI GGATC 1 cut(s) 583
AcoI YGGCCR 2 cut(s) 37, 51
AcsI RAATTY 7 cut(s) 31, 127, 178, 436, 754, 784, 1499
AcuI CTGAAG 1 cut(s) 1545
AfiI CCNNNNNNNGG 9 cut(s) 56, 155, 167, 281, 344, 548, 638, 806, 824
AgeI ACCGGT 1 cut(s) 1308
AgsI TTSAA 8 cut(s) 436, 499, 1010, 1172, 1498, 1550, 1610, 1664
AjnI CCWGG 4 cut(s) 166, 265, 406, 1437
AluBI AGCT 4 cut(s) 44, 72, 734, 1069
AluI AGCT 4 cut(s) 44, 72, 734, 1069
Alw21I GWGCWC 1 cut(s) 651
Alw26I GTCTC 2 cut(s) 843, 1281
Alw44I GTGCAC 1 cut(s) 647
AlwI GGATC 1 cut(s) 583
AoxI GGCC 7 cut(s) 37, 51, 464, 663, 1095, 1110, 1588
ApaLI GTGCAC 1 cut(s) 647
ApeKI GCWGC 3 cut(s) 94, 326, 596
ApoI RAATTY 7 cut(s) 31, 127, 178, 436, 754, 784, 1499
ArsI GACNNNNNNTTYG 2 cut(s) 1318, 1350
AsiGI ACCGGT 1 cut(s) 1308
Asp700I GAANNNNTTC 2 cut(s) 769, 1466
AspS9I GGNCC 7 cut(s) 158, 251, 293, 464, 554, 1306, 1588
AsuC2I CCSGG 2 cut(s) 50, 1570
AsuHPI GGTGA 3 cut(s) 17, 38, 1468
AsuII TTCGAA 1 cut(s) 752
AvaII GGWCC 5 cut(s) 158, 251, 293, 554, 1306
AxyI CCTNAGG 1 cut(s) 591
BaeGI GKGCMC 2 cut(s) 228, 651
BbsI GAAGAC 2 cut(s) 694, 1551
Bbv12I GWGCWC 1 cut(s) 651
BbvCI CCTCAGC 1 cut(s) 162
BbvI GCAGC 3 cut(s) 106, 313, 583
BccI CCATC 6 cut(s) 88, 124, 148, 1051, 1156, 1195
BceAI ACGGC 1 cut(s) 1575
BciT130I CCWGG 4 cut(s) 168, 267, 408, 1439
BcnI CCSGG 2 cut(s) 50, 1570
BcoDI GTCTC 2 cut(s) 843, 1281
BfaI CTAG 2 cut(s) 276, 1696
BfmI CTRYAG 3 cut(s) 597, 1388, 1509
BglI GCCNNNNNGGC 1 cut(s) 472
BglII AGATCT 1 cut(s) 974
BisI GCNGC 3 cut(s) 95, 327, 597
BlpI GCTNAGC 1 cut(s) 908
BlsI GCNGC 3 cut(s) 96, 328, 598
Bme1390I CCNGG 6 cut(s) 50, 168, 267, 408, 1439, 1570
Bme18I GGWCC 5 cut(s) 158, 251, 293, 554, 1306
BmgT120I GGNCC 7 cut(s) 158, 251, 293, 464, 554, 1306, 1588
BmiI GGNNCC 5 cut(s) 159, 244, 253, 1377, 1589
BmrFI CCNGG 6 cut(s) 50, 168, 267, 408, 1439, 1570
BmsI GCATC 2 cut(s) 192, 805
BpiI GAAGAC 2 cut(s) 694, 1551
Bpu10I CCTNAGC 1 cut(s) 162
Bpu1102I GCTNAGC 1 cut(s) 908
Bpu14I TTCGAA 1 cut(s) 752
BpuEI CTTGAG 1 cut(s) 1118
BpuMI CCSGG 2 cut(s) 50, 1570
BsaJI CCNNGG 3 cut(s) 247, 547, 706
BsaWI WCCGGW 1 cut(s) 1308
BsaXI ACNNNNNCTCC 4 cut(s) 1158, 1188, 1226, 1256
Bsc4I CCNNNNNNNGG 9 cut(s) 56, 155, 167, 281, 344, 548, 638, 806, 824
Bse118I RCCGGY 2 cut(s) 53, 1308
Bse21I CCTNAGG 1 cut(s) 591
Bse3DI GCAATG 2 cut(s) 919, 1654
BseBI CCWGG 4 cut(s) 168, 267, 408, 1439
BseDI CCNNGG 3 cut(s) 247, 547, 706
BseGI GGATG 1 cut(s) 796
BseLI CCNNNNNNNGG 9 cut(s) 56, 155, 167, 281, 344, 548, 638, 806, 824
BseMI GCAATG 2 cut(s) 919, 1654
BseMII CTCAG 6 cut(s) 176, 605, 854, 1149, 1170, 1215
BseRI GAGGAG 2 cut(s) 75, 1247
BseSI GKGCMC 2 cut(s) 228, 651
BseX3I CGGCCG 1 cut(s) 51
BseXI GCAGC 3 cut(s) 106, 313, 583
Bsh1285I CGRYCG 1 cut(s) 54
BshFI GGCC 7 cut(s) 39, 53, 466, 665, 1097, 1112, 1590
BshTI ACCGGT 1 cut(s) 1308
BsiEI CGRYCG 1 cut(s) 54
BsiHKAI GWGCWC 1 cut(s) 651
BsiSI CCGG 6 cut(s) 36, 50, 54, 666, 1309, 1569
BslFI GGGAC 3 cut(s) 171, 237, 724
BslI CCNNNNNNNGG 9 cut(s) 56, 155, 167, 281, 344, 548, 638, 806, 824
BsmAI GTCTC 2 cut(s) 843, 1281
BsmFI GGGAC 3 cut(s) 171, 237, 724
BsnI GGCC 7 cut(s) 39, 53, 466, 665, 1097, 1112, 1590
Bsp119I TTCGAA 1 cut(s) 752
Bsp1286I GDGCHC 2 cut(s) 228, 651
Bsp143I GATC 5 cut(s) 575, 974, 1262, 1328, 1686
Bsp1720I GCTNAGC 1 cut(s) 908
BspACI CCGC 3 cut(s) 57, 485, 818
BspANI GGCC 7 cut(s) 39, 53, 466, 665, 1097, 1112, 1590
BspCNI CTCAG 6 cut(s) 175, 604, 853, 1150, 1171, 1214
BspHI TCATGA 1 cut(s) 174
BspLI GGNNCC 5 cut(s) 159, 244, 253, 1377, 1589
BspMAI CTGCAG 2 cut(s) 601, 1392
BspPI GGATC 1 cut(s) 583
BspQI GCTCTTC 1 cut(s) 942
BspT104I TTCGAA 1 cut(s) 752
BsrDI GCAATG 2 cut(s) 919, 1654
BsrFI RCCGGY 2 cut(s) 53, 1308
BssAI RCCGGY 2 cut(s) 53, 1308
BssECI CCNNGG 3 cut(s) 247, 547, 706
BssMI GATC 5 cut(s) 575, 974, 1262, 1328, 1686
BssT1I CCWWGG 2 cut(s) 247, 547
Bst2UI CCWGG 4 cut(s) 168, 267, 408, 1439
Bst4CI ACNGT 2 cut(s) 1014, 1510
Bst6I CTCTTC 3 cut(s) 942, 1222, 1671
BstAPI GCANNNNNTGC 1 cut(s) 103
BstBI TTCGAA 1 cut(s) 752
BstC8I GCNNGC 2 cut(s) 55, 1155
BstDEI CTNAG 9 cut(s) 162, 591, 840, 908, 1070, 1158, 1179, 1201, 1400
BstENI CCTNNNNNAGG 1 cut(s) 546
BstF5I GGATG 1 cut(s) 796
BstKTI GATC 5 cut(s) 578, 977, 1265, 1331, 1689
BstMAI GTCTC 2 cut(s) 843, 1281
BstMBI GATC 5 cut(s) 575, 974, 1262, 1328, 1686
BstMCI CGRYCG 1 cut(s) 54
BstMWI GCNNNNNNNGC 6 cut(s) 50, 103, 232, 472, 593, 623
BstNI CCWGG 4 cut(s) 168, 267, 408, 1439
BstSCI CCNGG 6 cut(s) 48, 166, 265, 406, 1437, 1568
BstSFI CTRYAG 3 cut(s) 597, 1388, 1509
BstSLI GKGCMC 2 cut(s) 228, 651
BstV1I GCAGC 3 cut(s) 106, 313, 583
BstV2I GAAGAC 2 cut(s) 694, 1551
BstX2I RGATCY 1 cut(s) 974
BstYI RGATCY 1 cut(s) 974
BstZI CGGCCG 1 cut(s) 51
Bsu36I CCTNAGG 1 cut(s) 591
BsuRI GGCC 7 cut(s) 39, 53, 466, 665, 1097, 1112, 1590
BtsCI GGATG 1 cut(s) 796
Cac8I GCNNGC 2 cut(s) 55, 1155
CciI TCATGA 1 cut(s) 174
Cfr10I RCCGGY 2 cut(s) 53, 1308
Cfr13I GGNCC 7 cut(s) 158, 251, 293, 464, 554, 1306, 1588
CsiI ACCWGGT 2 cut(s) 265, 406
CspAI ACCGGT 1 cut(s) 1308
CviAII CATG 6 cut(s) 132, 175, 229, 1245, 1453, 1603
DdeI CTNAG 9 cut(s) 162, 591, 840, 908, 1070, 1158, 1179, 1201, 1400
DpnI GATC 5 cut(s) 577, 976, 1264, 1330, 1688
DpnII GATC 5 cut(s) 575, 974, 1262, 1328, 1686
DraI TTTAAA 1 cut(s) 1474
EaeI YGGCCR 2 cut(s) 37, 51
EagI CGGCCG 1 cut(s) 51
Eam1104I CTCTTC 3 cut(s) 942, 1222, 1671
EarI CTCTTC 3 cut(s) 942, 1222, 1671
EciI GGCGGA 1 cut(s) 474
EclXI CGGCCG 1 cut(s) 51
Eco130I CCWWGG 2 cut(s) 247, 547
Eco32I GATATC 1 cut(s) 1411
Eco47I GGWCC 5 cut(s) 158, 251, 293, 554, 1306
Eco52I CGGCCG 1 cut(s) 51
Eco57I CTGAAG 1 cut(s) 1545
Eco81I CCTNAGG 1 cut(s) 591
EcoNI CCTNNNNNAGG 1 cut(s) 546
EcoO109I RGGNCCY 1 cut(s) 158
EcoRI GAATTC 1 cut(s) 754
EcoRII CCWGG 4 cut(s) 166, 265, 406, 1437
EcoRV GATATC 1 cut(s) 1411
EcoT14I CCWWGG 2 cut(s) 247, 547
ErhI CCWWGG 2 cut(s) 247, 547
FaeI CATG 6 cut(s) 135, 178, 232, 1248, 1456, 1606
FaqI GGGAC 3 cut(s) 171, 237, 724
FatI CATG 6 cut(s) 131, 174, 228, 1244, 1452, 1602
FauNDI CATATG 2 cut(s) 216, 469
Fnu4HI GCNGC 3 cut(s) 95, 327, 597
FokI GGATG 1 cut(s) 783
Fsp4HI GCNGC 3 cut(s) 95, 327, 597
FspBI CTAG 2 cut(s) 276, 1696
GluI GCNGC 3 cut(s) 95, 327, 597
HaeIII GGCC 7 cut(s) 39, 53, 466, 665, 1097, 1112, 1590
HapII CCGG 6 cut(s) 36, 50, 54, 666, 1309, 1569
Hin1II CATG 6 cut(s) 135, 178, 232, 1248, 1456, 1606
HpaII CCGG 6 cut(s) 36, 50, 54, 666, 1309, 1569
HphI GGTGA 3 cut(s) 17, 38, 1468
Hpy166II GTNNAC 3 cut(s) 296, 649, 745
Hpy188I TCNGA 9 cut(s) 201, 707, 760, 834, 1093, 1204, 1260, 1338, 1408
Hpy188III TCNNGA 4 cut(s) 175, 941, 1414, 1484
Hpy8I GTNNAC 3 cut(s) 296, 649, 745
HpyAV CCTTC 9 cut(s) 159, 913, 917, 1016, 1116, 1297, 1333, 1368, 1532
HpyCH4III ACNGT 2 cut(s) 1014, 1510
HpyCH4IV ACGT 2 cut(s) 699, 1027
HpyF10VI GCNNNNNNNGC 6 cut(s) 50, 103, 232, 472, 593, 623
HpyF3I CTNAG 9 cut(s) 162, 591, 840, 908, 1070, 1158, 1179, 1201, 1400
HpySE526I ACGT 2 cut(s) 699, 1027
Hsp92II CATG 6 cut(s) 135, 178, 232, 1248, 1456, 1606
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
Kzo9I GATC 5 cut(s) 575, 974, 1262, 1328, 1686
LguI GCTCTTC 1 cut(s) 942
LmnI GCTCC 3 cut(s) 82, 1126, 1375
Lsp1109I GCAGC 3 cut(s) 106, 313, 583
LweI GCATC 2 cut(s) 192, 805
MabI ACCWGGT 2 cut(s) 265, 406
MaeI CTAG 2 cut(s) 276, 1696
MaeII ACGT 2 cut(s) 699, 1027
MaeIII GTNAC 4 cut(s) 3, 1456, 1592, 1619
MalI GATC 5 cut(s) 577, 976, 1264, 1330, 1688
MboI GATC 5 cut(s) 575, 974, 1262, 1328, 1686
MfeI CAATTG 1 cut(s) 413
MflI RGATCY 1 cut(s) 974
MhlI GDGCHC 2 cut(s) 228, 651
MlyI GAGTC 2 cut(s) 938, 1082
MmeI TCCRAC 1 cut(s) 262
MroNI GCCGGC 1 cut(s) 53
MroXI GAANNNNTTC 2 cut(s) 769, 1466
MseI TTAA 5 cut(s) 111, 342, 1281, 1473, 1518
MslI CAYNNNNRTG 1 cut(s) 219
MspI CCGG 6 cut(s) 36, 50, 54, 666, 1309, 1569
MspR9I CCNGG 6 cut(s) 50, 168, 267, 408, 1439, 1570
MunI CAATTG 1 cut(s) 413
MvaI CCWGG 4 cut(s) 168, 267, 408, 1439
MwoI GCNNNNNNNGC 6 cut(s) 50, 103, 232, 472, 593, 623
NaeI GCCGGC 1 cut(s) 55
NciI CCSGG 2 cut(s) 50, 1570
NdeI CATATG 2 cut(s) 216, 469
NdeII GATC 5 cut(s) 575, 974, 1262, 1328, 1686
NgoMIV GCCGGC 1 cut(s) 53
NlaIII CATG 6 cut(s) 135, 178, 232, 1248, 1456, 1606
NlaIV GGNNCC 5 cut(s) 159, 244, 253, 1377, 1589
NmuCI GTSAC 2 cut(s) 1456, 1592
NspV TTCGAA 1 cut(s) 752
PagI TCATGA 1 cut(s) 174
PciSI GCTCTTC 1 cut(s) 942
PdiI GCCGGC 1 cut(s) 55
PdmI GAANNNNTTC 2 cut(s) 769, 1466
PfeI GAWTC 8 cut(s) 370, 451, 505, 685, 952, 1141, 1205, 1343
PinAI ACCGGT 1 cut(s) 1308
PkrI GCNGC 3 cut(s) 96, 328, 598
PleI GAGTC 2 cut(s) 938, 1082
PpsI GAGTC 2 cut(s) 938, 1082
PpuMI RGGWCCY 1 cut(s) 158
PsiI TTATAA 2 cut(s) 791, 1632
Psp5II RGGWCCY 1 cut(s) 158
Psp6I CCWGG 4 cut(s) 166, 265, 406, 1437
PspGI CCWGG 4 cut(s) 166, 265, 406, 1437
PspN4I GGNNCC 5 cut(s) 159, 244, 253, 1377, 1589
PspPI GGNCC 7 cut(s) 158, 251, 293, 464, 554, 1306, 1588
PspPPI RGGWCCY 1 cut(s) 158
PstI CTGCAG 2 cut(s) 601, 1392
PsuI RGATCY 1 cut(s) 974
RseI CAYNNNNRTG 1 cut(s) 219
SapI GCTCTTC 1 cut(s) 942
SaqAI TTAA 5 cut(s) 111, 342, 1281, 1473, 1518
SatI GCNGC 3 cut(s) 95, 327, 597
Sau3AI GATC 5 cut(s) 575, 974, 1262, 1328, 1686
Sau96I GGNCC 7 cut(s) 158, 251, 293, 464, 554, 1306, 1588
SchI GAGTC 2 cut(s) 938, 1082
ScrFI CCNGG 6 cut(s) 50, 168, 267, 408, 1439, 1570
SduI GDGCHC 2 cut(s) 228, 651
SexAI ACCWGGT 2 cut(s) 265, 406
SfaNI GCATC 2 cut(s) 192, 805
SfcI CTRYAG 3 cut(s) 597, 1388, 1509
SfuI TTCGAA 1 cut(s) 752
SinI GGWCC 5 cut(s) 158, 251, 293, 554, 1306
SmiMI CAYNNNNRTG 1 cut(s) 219
SmlI CTYRAG 1 cut(s) 1133
SmoI CTYRAG 1 cut(s) 1133
SsiI CCGC 3 cut(s) 57, 485, 818
SspI AATATT 1 cut(s) 460
SspMI CTAG 2 cut(s) 276, 1696
StyD4I CCNGG 6 cut(s) 48, 166, 265, 406, 1437, 1568
StyI CCWWGG 2 cut(s) 247, 547
TaaI ACNGT 2 cut(s) 1014, 1510
TaiI ACGT 2 cut(s) 702, 1030
TaqI TCGA 2 cut(s) 152, 752
TfiI GAWTC 8 cut(s) 370, 451, 505, 685, 952, 1141, 1205, 1343
Tru1I TTAA 5 cut(s) 111, 342, 1281, 1473, 1518
Tru9I TTAA 5 cut(s) 111, 342, 1281, 1473, 1518
TseFI GTSAC 2 cut(s) 1456, 1592
TseI GCWGC 3 cut(s) 94, 326, 596
Tsp45I GTSAC 2 cut(s) 1456, 1592
TspGWI ACGGA 1 cut(s) 1010
VneI GTGCAC 1 cut(s) 647
VpaK11BI GGWCC 5 cut(s) 158, 251, 293, 554, 1306
XagI CCTNNNNNAGG 1 cut(s) 546
XapI RAATTY 7 cut(s) 31, 127, 178, 436, 754, 784, 1499
XmnI GAANNNNTTC 2 cut(s) 769, 1466
XspI CTAG 2 cut(s) 276, 1696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.