RLG00000022881

Nuclear fragile X mental retardation-interacting protein 1 (NUFIP1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0005893
Physical Location & Seq
Reverse (-)
13991828 .. 13998050
6223 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022881

Sequence Viewer

Length: 1713 bp
ATGTTACCTCACTATAATTCCTTTCACCAAAATTCCGGCCAAGCTCATCCGGCCGGCGGTAATGGTGGTAGCTCTGCTCCATCTCCTCAACAGCAGCAGCAGGGTATGCCATTTAATCCATCAGTTGCAAATTCATGTAATATGTTGCCACCTTCGATGGGACCTCAGCCCGGTTTCATGAGTTTATCTAACAACCTTCTTCCGATGCAAAATAACCATATGCGTATGCCCATGTCCCAATTCGGTTCCCTTGGTCCCATATCTCAACCAGGTTATCCCAGTGTTGGATTTTGTGGTCCACAGAATAATGCACTCAATATGAACTTGGCTGCTTCCTATCCATTTAACATGCAGTTCTGCAATATGGGGCAGAATCTGAATCAAGTAAATCAAAATATGGGTTTTCTACCTGGTCAATTGTTTGGACATAACCTGTTGAATTTACAGCAGATGAATCAAAATATCGGGCCATATGGGCAATTCTGTTCTCCGAATCTGTTGAATAGGAATCAGGTTGTGCCAATGCAAATGCAAAACCCTTGCCAAGGTGGTCCTAATTATGCATTTGGGGGGTCAAATCAAGCACCTCAGGCTGCAGTTCTGCAAAATCCTGCTTTTTTTGCAACTCCTAATTCTGGTAATGTGCACTCTCCAAATCAGGCCGGACAACAAATCAACCAGAATCAGCAAAACAACGTCTTCCGAGGGACACAGGGAAATCATATAAAAGCTGGTGGCATTTACAATTCGAACTCTGACAGAAAAACTTCACCAAGCAAAAATTTTATAAAGCATCCAAAACGAGGGTCGCAGCCGCAAGGGGGATTTCAGAATTCTCAGTTTCATCGCATCAAAAATGCAAAGGGAAAGTTTGCTTTTCCCAACAGGCAAAACAGAAAAGACACACACACAAAAGCAGGGCTAAGCAATGAGAAGGAAGGAAAATCTGCTCTTCCCGACTCTATGAATCAAGGCAGGGAAAGGAAAAGATCTCTTTCGTTGTCCTATACGGAACAAGAAGTTCAACTGTGGCGTGAAGAACGTAGGAAGAACTACCCATCAAACACCAACATAGAGAAGAAGCTAAGTGAAAAGATGATAGACTCGGAGGCCATTGAGAGAGAGGCCAAAGCACGAAGGGAGCAACTCAAGGAGATTCTTACAAAGCAGGCTGAGATGGGAGTTGAAGTCGCTGAGATACCGTCTCACTACCTCTCAGATTCAGAAAAACAAGGGAATGGAAGAGAGGAGAATAAGTCATGGACTAACAATCGGAGATCACAAAACAAGTTCGTTAAGAGAGACAAATATGATAAGAAGGACCGGTTTGCAAAGAGGCAAAGATCAAGTCAGAAGGATTCATCTAATGACCCTTCATTCAGCAAAAGGGAGCCAACCTTACTGCAGAAACTTCTTAGTTCAGATATCAAGAGAGATAAAAGTCGCTTATGCCAGGTGTTTAGGTTCATGGTGACAAACTCTTTCTTTAAAGATTTTCCTGACAAACCTTTGAAATTTCCTACTGTGGTAGTTAAAGAAAGTGTGGTGGAGGAAGGTGTTGTTGAAGAGTTGTCTTCAGTTGCCGGGAAAGGTGCTTATGGGGGCCGTGACAAGTCCATGTTTGAAAAAGTTGGTAACAATGATTATAAAGATTGTTGTTACACTGATGATTGTGTTGAAGATGAGGAAGAAGAGGGAGAGATCATCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko05164 map03015 map05164
Pfam Domains
Protein Families
NUFIP1

Protein Analysis

571

Amino Acids

63.88

Weight (kDa)

9.34

Isoelectric Point (pI)

53.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUFIP1 PF10453 333 - 365 3.9e-10 FMR1-interacting protein 1 (NUFIP1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 788, 1647
AciI CCGC 2 cut(s) 57, 815
AcoI YGGCCR 2 cut(s) 37, 51
AcsI RAATTY 6 cut(s) 31, 130, 439, 781, 832, 1514
AcuI CTGAAG 1 cut(s) 1560
AfiI CCNNNNNNNGG 8 cut(s) 56, 158, 170, 284, 545, 635, 803, 821
AgeI ACCGGT 1 cut(s) 1323
AgsI TTSAA 8 cut(s) 439, 502, 1025, 1187, 1513, 1565, 1625, 1679
AjnI CCWGG 3 cut(s) 268, 409, 1452
AluBI AGCT 4 cut(s) 44, 72, 731, 1084
AluI AGCT 4 cut(s) 44, 72, 731, 1084
Alw21I GWGCWC 1 cut(s) 648
Alw26I GTCTC 2 cut(s) 1209, 1296
Alw44I GTGCAC 1 cut(s) 644
AlwNI CAGNNNCTG 1 cut(s) 376
AoxI GGCC 7 cut(s) 37, 51, 467, 660, 1110, 1125, 1603
ApaLI GTGCAC 1 cut(s) 644
ApeKI GCWGC 5 cut(s) 94, 97, 329, 593, 811
ApoI RAATTY 6 cut(s) 31, 130, 439, 781, 832, 1514
ArsI GACNNNNNNTTYG 4 cut(s) 791, 823, 1333, 1365
AsiGI ACCGGT 1 cut(s) 1323
Asp700I GAANNNNTTC 1 cut(s) 766
AspS9I GGNCC 7 cut(s) 161, 254, 296, 467, 551, 1321, 1603
AsuC2I CCSGG 2 cut(s) 171, 1585
AsuHPI GGTGA 3 cut(s) 17, 762, 1483
AsuII TTCGAA 1 cut(s) 749
AvaII GGWCC 5 cut(s) 161, 254, 296, 551, 1321
AxyI CCTNAGG 1 cut(s) 588
BaeGI GKGCMC 1 cut(s) 648
BbsI GAAGAC 2 cut(s) 691, 1566
Bbv12I GWGCWC 1 cut(s) 648
BbvCI CCTCAGC 1 cut(s) 165
BbvI GCAGC 5 cut(s) 106, 109, 316, 580, 823
BccI CCATC 5 cut(s) 88, 127, 151, 1066, 1171
BceAI ACGGC 1 cut(s) 1590
BciT130I CCWGG 3 cut(s) 270, 411, 1454
BcnI CCSGG 2 cut(s) 171, 1585
BcoDI GTCTC 2 cut(s) 1209, 1296
BfaI CTAG 1 cut(s) 1711
BfmI CTRYAG 2 cut(s) 594, 1403
BglI GCCNNNNNGGC 1 cut(s) 475
BglII AGATCT 1 cut(s) 989
BisI GCNGC 6 cut(s) 95, 98, 330, 594, 812, 815
BlpI GCTNAGC 1 cut(s) 923
BlsI GCNGC 6 cut(s) 96, 99, 331, 595, 813, 816
Bme1390I CCNGG 5 cut(s) 171, 270, 411, 1454, 1585
Bme18I GGWCC 5 cut(s) 161, 254, 296, 551, 1321
BmgT120I GGNCC 7 cut(s) 161, 254, 296, 467, 551, 1321, 1603
BmiI GGNNCC 5 cut(s) 162, 247, 256, 1392, 1604
BmrFI CCNGG 5 cut(s) 171, 270, 411, 1454, 1585
BmrI ACTGGG 1 cut(s) 273
BmsI GCATC 3 cut(s) 195, 802, 858
BmuI ACTGGG 1 cut(s) 273
BpiI GAAGAC 2 cut(s) 691, 1566
Bpu10I CCTNAGC 1 cut(s) 165
Bpu1102I GCTNAGC 1 cut(s) 923
Bpu14I TTCGAA 1 cut(s) 749
BpuEI CTTGAG 1 cut(s) 1133
BpuMI CCSGG 2 cut(s) 171, 1585
BsaJI CCNNGG 3 cut(s) 250, 544, 703
BsaWI WCCGGW 1 cut(s) 1323
BsaXI ACNNNNNCTCC 4 cut(s) 1173, 1203, 1241, 1271
Bsc4I CCNNNNNNNGG 8 cut(s) 56, 158, 170, 284, 545, 635, 803, 821
Bse118I RCCGGY 2 cut(s) 53, 1323
Bse1I ACTGG 1 cut(s) 279
Bse21I CCTNAGG 1 cut(s) 588
Bse3DI GCAATG 1 cut(s) 934
BseBI CCWGG 3 cut(s) 270, 411, 1454
BseDI CCNNGG 3 cut(s) 250, 544, 703
BseGI GGATG 2 cut(s) 46, 793
BseLI CCNNNNNNNGG 8 cut(s) 56, 158, 170, 284, 545, 635, 803, 821
BseMI GCAATG 1 cut(s) 934
BseMII CTCAG 6 cut(s) 179, 602, 851, 1164, 1185, 1230
BseNI ACTGG 1 cut(s) 279
BseRI GAGGAG 2 cut(s) 75, 1262
BseSI GKGCMC 1 cut(s) 648
BseX3I CGGCCG 1 cut(s) 51
BseXI GCAGC 5 cut(s) 106, 109, 316, 580, 823
Bsh1285I CGRYCG 1 cut(s) 54
BshFI GGCC 7 cut(s) 39, 53, 469, 662, 1112, 1127, 1605
BshTI ACCGGT 1 cut(s) 1323
BsiEI CGRYCG 1 cut(s) 54
BsiHKAI GWGCWC 1 cut(s) 648
BsiSI CCGG 7 cut(s) 36, 50, 54, 171, 663, 1324, 1584
BslFI GGGAC 4 cut(s) 174, 220, 240, 721
BslI CCNNNNNNNGG 8 cut(s) 56, 158, 170, 284, 545, 635, 803, 821
BsmAI GTCTC 2 cut(s) 1209, 1296
BsmBI CGTCTC 1 cut(s) 1209
BsmFI GGGAC 4 cut(s) 174, 220, 240, 721
BsnI GGCC 7 cut(s) 39, 53, 469, 662, 1112, 1127, 1605
Bsp119I TTCGAA 1 cut(s) 749
Bsp1286I GDGCHC 1 cut(s) 648
Bsp143I GATC 4 cut(s) 989, 1277, 1343, 1701
Bsp1720I GCTNAGC 1 cut(s) 923
BspACI CCGC 2 cut(s) 57, 815
BspANI GGCC 7 cut(s) 39, 53, 469, 662, 1112, 1127, 1605
BspCNI CTCAG 6 cut(s) 178, 601, 850, 1165, 1186, 1229
BspHI TCATGA 1 cut(s) 177
BspLI GGNNCC 5 cut(s) 162, 247, 256, 1392, 1604
BspMAI CTGCAG 2 cut(s) 598, 1407
BspQI GCTCTTC 1 cut(s) 957
BspT104I TTCGAA 1 cut(s) 749
BsrDI GCAATG 1 cut(s) 934
BsrFI RCCGGY 2 cut(s) 53, 1323
BsrI ACTGG 1 cut(s) 279
BssAI RCCGGY 2 cut(s) 53, 1323
BssECI CCNNGG 3 cut(s) 250, 544, 703
BssMI GATC 4 cut(s) 989, 1277, 1343, 1701
BssT1I CCWWGG 2 cut(s) 250, 544
Bst2UI CCWGG 3 cut(s) 270, 411, 1454
Bst4CI ACNGT 3 cut(s) 1029, 1203, 1525
Bst6I CTCTTC 4 cut(s) 957, 1237, 1560, 1686
BstAPI GCANNNNNTGC 1 cut(s) 106
BstBI TTCGAA 1 cut(s) 749
BstC8I GCNNGC 2 cut(s) 55, 1170
BstDEI CTNAG 9 cut(s) 165, 588, 837, 923, 1085, 1173, 1194, 1216, 1415
BstENI CCTNNNNNAGG 1 cut(s) 543
BstF5I GGATG 2 cut(s) 46, 793
BstKTI GATC 4 cut(s) 992, 1280, 1346, 1704
BstMAI GTCTC 2 cut(s) 1209, 1296
BstMBI GATC 4 cut(s) 989, 1277, 1343, 1701
BstMCI CGRYCG 1 cut(s) 54
BstMWI GCNNNNNNNGC 5 cut(s) 50, 106, 475, 590, 620
BstNI CCWGG 3 cut(s) 270, 411, 1454
BstNSI RCATGY 1 cut(s) 352
BstSCI CCNGG 5 cut(s) 169, 268, 409, 1452, 1583
BstSFI CTRYAG 2 cut(s) 594, 1403
BstSLI GKGCMC 1 cut(s) 648
BstV1I GCAGC 5 cut(s) 106, 109, 316, 580, 823
BstV2I GAAGAC 2 cut(s) 691, 1566
BstX2I RGATCY 1 cut(s) 989
BstYI RGATCY 1 cut(s) 989
BstZI CGGCCG 1 cut(s) 51
Bsu36I CCTNAGG 1 cut(s) 588
BsuRI GGCC 7 cut(s) 39, 53, 469, 662, 1112, 1127, 1605
BtgZI GCGATG 1 cut(s) 830
BtsCI GGATG 2 cut(s) 46, 793
BtsIMutI CAGTG 2 cut(s) 286, 1662
Cac8I GCNNGC 2 cut(s) 55, 1170
CaiI CAGNNNCTG 1 cut(s) 376
CciI TCATGA 1 cut(s) 177
Cfr10I RCCGGY 2 cut(s) 53, 1323
Cfr13I GGNCC 7 cut(s) 161, 254, 296, 467, 551, 1321, 1603
CsiI ACCWGGT 2 cut(s) 268, 409
CspAI ACCGGT 1 cut(s) 1323
CviAII CATG 7 cut(s) 135, 178, 232, 349, 1260, 1468, 1618
DdeI CTNAG 9 cut(s) 165, 588, 837, 923, 1085, 1173, 1194, 1216, 1415
DpnI GATC 4 cut(s) 991, 1279, 1345, 1703
DpnII GATC 4 cut(s) 989, 1277, 1343, 1701
DraI TTTAAA 1 cut(s) 1489
EaeI YGGCCR 2 cut(s) 37, 51
EagI CGGCCG 1 cut(s) 51
Eam1104I CTCTTC 4 cut(s) 957, 1237, 1560, 1686
EarI CTCTTC 4 cut(s) 957, 1237, 1560, 1686
EclXI CGGCCG 1 cut(s) 51
Eco130I CCWWGG 2 cut(s) 250, 544
Eco32I GATATC 1 cut(s) 1426
Eco47I GGWCC 5 cut(s) 161, 254, 296, 551, 1321
Eco52I CGGCCG 1 cut(s) 51
Eco57I CTGAAG 1 cut(s) 1560
Eco81I CCTNAGG 1 cut(s) 588
EcoNI CCTNNNNNAGG 1 cut(s) 543
EcoO109I RGGNCCY 1 cut(s) 161
EcoRI GAATTC 1 cut(s) 832
EcoRII CCWGG 3 cut(s) 268, 409, 1452
EcoRV GATATC 1 cut(s) 1426
EcoT14I CCWWGG 2 cut(s) 250, 544
EcoT22I ATGCAT 1 cut(s) 565
ErhI CCWWGG 2 cut(s) 250, 544
Esp3I CGTCTC 1 cut(s) 1209
FaeI CATG 7 cut(s) 138, 181, 235, 352, 1263, 1471, 1621
FaqI GGGAC 4 cut(s) 174, 220, 240, 721
FatI CATG 7 cut(s) 134, 177, 231, 348, 1259, 1467, 1617
FauNDI CATATG 2 cut(s) 219, 472
Fnu4HI GCNGC 6 cut(s) 95, 98, 330, 594, 812, 815
FokI GGATG 2 cut(s) 33, 780
Fsp4HI GCNGC 6 cut(s) 95, 98, 330, 594, 812, 815
FspBI CTAG 1 cut(s) 1711
GluI GCNGC 6 cut(s) 95, 98, 330, 594, 812, 815
HaeIII GGCC 7 cut(s) 39, 53, 469, 662, 1112, 1127, 1605
HapII CCGG 7 cut(s) 36, 50, 54, 171, 663, 1324, 1584
Hin1II CATG 7 cut(s) 138, 181, 235, 352, 1263, 1471, 1621
HpaII CCGG 7 cut(s) 36, 50, 54, 171, 663, 1324, 1584
HphI GGTGA 3 cut(s) 17, 762, 1483
Hpy166II GTNNAC 2 cut(s) 299, 646
Hpy188III TCNNGA 4 cut(s) 178, 956, 1429, 1499
Hpy8I GTNNAC 2 cut(s) 299, 646
HpyAV CCTTC 9 cut(s) 162, 206, 928, 932, 1131, 1312, 1348, 1383, 1547
HpyCH4III ACNGT 3 cut(s) 1029, 1203, 1525
HpyCH4IV ACGT 2 cut(s) 696, 1042
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 106, 475, 590, 620
HpyF3I CTNAG 9 cut(s) 165, 588, 837, 923, 1085, 1173, 1194, 1216, 1415
HpySE526I ACGT 2 cut(s) 696, 1042
Hsp92II CATG 7 cut(s) 138, 181, 235, 352, 1263, 1471, 1621
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
Kzo9I GATC 4 cut(s) 989, 1277, 1343, 1701
LguI GCTCTTC 1 cut(s) 957
LmnI GCTCC 3 cut(s) 82, 1141, 1390
Lsp1109I GCAGC 5 cut(s) 106, 109, 316, 580, 823
LweI GCATC 3 cut(s) 195, 802, 858
MabI ACCWGGT 2 cut(s) 268, 409
MaeI CTAG 1 cut(s) 1711
MaeII ACGT 2 cut(s) 696, 1042
MaeIII GTNAC 5 cut(s) 3, 1471, 1607, 1634, 1658
MalI GATC 4 cut(s) 991, 1279, 1345, 1703
MboI GATC 4 cut(s) 989, 1277, 1343, 1701
MfeI CAATTG 1 cut(s) 416
MflI RGATCY 1 cut(s) 989
MhlI GDGCHC 1 cut(s) 648
MlyI GAGTC 2 cut(s) 953, 1097
MmeI TCCRAC 1 cut(s) 265
Mph1103I ATGCAT 1 cut(s) 565
MroNI GCCGGC 1 cut(s) 53
MroXI GAANNNNTTC 1 cut(s) 766
MseI TTAA 5 cut(s) 114, 345, 1296, 1488, 1533
MspI CCGG 7 cut(s) 36, 50, 54, 171, 663, 1324, 1584
MspR9I CCNGG 5 cut(s) 171, 270, 411, 1454, 1585
MunI CAATTG 1 cut(s) 416
MvaI CCWGG 3 cut(s) 270, 411, 1454
MwoI GCNNNNNNNGC 5 cut(s) 50, 106, 475, 590, 620
NaeI GCCGGC 1 cut(s) 55
NciI CCSGG 2 cut(s) 171, 1585
NdeI CATATG 2 cut(s) 219, 472
NdeII GATC 4 cut(s) 989, 1277, 1343, 1701
NgoMIV GCCGGC 1 cut(s) 53
NlaIII CATG 7 cut(s) 138, 181, 235, 352, 1263, 1471, 1621
NlaIV GGNNCC 5 cut(s) 162, 247, 256, 1392, 1604
NmuCI GTSAC 2 cut(s) 1471, 1607
NsiI ATGCAT 1 cut(s) 565
NspI RCATGY 1 cut(s) 352
NspV TTCGAA 1 cut(s) 749
PagI TCATGA 1 cut(s) 177
PciSI GCTCTTC 1 cut(s) 957
PdiI GCCGGC 1 cut(s) 55
PdmI GAANNNNTTC 1 cut(s) 766
PinAI ACCGGT 1 cut(s) 1323
PkrI GCNGC 6 cut(s) 96, 99, 331, 595, 813, 816
PleI GAGTC 2 cut(s) 953, 1097
PpsI GAGTC 2 cut(s) 953, 1097
PpuMI RGGWCCY 1 cut(s) 161
PsiI TTATAA 2 cut(s) 788, 1647
Psp5II RGGWCCY 1 cut(s) 161
Psp6I CCWGG 3 cut(s) 268, 409, 1452
PspGI CCWGG 3 cut(s) 268, 409, 1452
PspN4I GGNNCC 5 cut(s) 162, 247, 256, 1392, 1604
PspPI GGNCC 7 cut(s) 161, 254, 296, 467, 551, 1321, 1603
PspPPI RGGWCCY 1 cut(s) 161
PstI CTGCAG 2 cut(s) 598, 1407
PstNI CAGNNNCTG 1 cut(s) 376
PsuI RGATCY 1 cut(s) 989
SapI GCTCTTC 1 cut(s) 957
SaqAI TTAA 5 cut(s) 114, 345, 1296, 1488, 1533
SatI GCNGC 6 cut(s) 95, 98, 330, 594, 812, 815
Sau3AI GATC 4 cut(s) 989, 1277, 1343, 1701
Sau96I GGNCC 7 cut(s) 161, 254, 296, 467, 551, 1321, 1603
SchI GAGTC 2 cut(s) 953, 1097
ScrFI CCNGG 5 cut(s) 171, 270, 411, 1454, 1585
SduI GDGCHC 1 cut(s) 648
SexAI ACCWGGT 2 cut(s) 268, 409
SfaNI GCATC 3 cut(s) 195, 802, 858
SfcI CTRYAG 2 cut(s) 594, 1403
SfuI TTCGAA 1 cut(s) 749
SinI GGWCC 5 cut(s) 161, 254, 296, 551, 1321
SmlI CTYRAG 1 cut(s) 1148
SmoI CTYRAG 1 cut(s) 1148
SsiI CCGC 2 cut(s) 57, 815
SspMI CTAG 1 cut(s) 1711
StyD4I CCNGG 5 cut(s) 169, 268, 409, 1452, 1583
StyI CCWWGG 2 cut(s) 250, 544
TaaI ACNGT 3 cut(s) 1029, 1203, 1525
TaiI ACGT 2 cut(s) 699, 1045
TaqI TCGA 2 cut(s) 155, 749
TauI GCSGC 1 cut(s) 817
Tru1I TTAA 5 cut(s) 114, 345, 1296, 1488, 1533
Tru9I TTAA 5 cut(s) 114, 345, 1296, 1488, 1533
TscAI CASTG 2 cut(s) 286, 1669
TseFI GTSAC 2 cut(s) 1471, 1607
TseI GCWGC 5 cut(s) 94, 97, 329, 593, 811
Tsp45I GTSAC 2 cut(s) 1471, 1607
TspDTI ATGAA 9 cut(s) 123, 166, 335, 467, 833, 980, 1350, 1365, 1456
TspGWI ACGGA 1 cut(s) 1025
TspRI CASTG 2 cut(s) 286, 1669
VneI GTGCAC 1 cut(s) 644
VpaK11BI GGWCC 5 cut(s) 161, 254, 296, 551, 1321
XagI CCTNNNNNAGG 1 cut(s) 543
XapI RAATTY 6 cut(s) 31, 130, 439, 781, 832, 1514
XceI RCATGY 1 cut(s) 352
XmnI GAANNNNTTC 1 cut(s) 766
XspI CTAG 1 cut(s) 1711
Zsp2I ATGCAT 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.