Rroxscaffold_6G00393380

Nuclear fragile X mental retardation-interacting protein 1 (NUFIP1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0005893
Physical Location & Seq
Reverse (-)
13414436 .. 13421510
7075 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00393380.1

Sequence Viewer

Length: 1686 bp
ATGTTACCTCACTATAATTCCTTTCACCAAAATTCCGGCCAAGCTCACCCGGCCGGCGGTAATGGTGGTAGCTCTGCTCCATCTCCTCAACAGCAGCAGGGTATGCCATTTAATCCATCAGTTGCAAATTCATGTAATATGCTGCCACCTTCGATGGGACCTCAGCCCGGTTTCATGAATTTATCTAACAACCTTCTTCCAATGCAAAGTAACCATATGCGTGTGCCCATGTCCCAATTCGGTTCCCTTGGTCCCATATCTCAACCAGGTTATCCCAGTGTTGGATTTTGTGGTCCACAGAATAATGCACTCAATATGAACTTGGCTGCTTCCTATCCATTTAACGGGCAGTTCTGCAATATGGGGCAGAATCTCAATCAAGTAAATCAAAATATGGGTTTTCTACCTGGTCAATTGTTTGGACATAACCTGTTGAATTTACAGCAGATGAATCAAAATATTGGGCCATATGGGCAATTCTGTTCGCCGAATCTGTTGAATGGGAATCAGGTTGTGCCAATGCAAATGCAAAACCCTTGCCAAGGTGGTCCTAATTATGCATTTGGAGGATCAAATCATGCACCTCAGGCTGCAGTTCTGCAAAATCCTGCTTTTTTTGCAACTCCTAATTCTGGTAATGTGCACTCTCCAAATCAGGCAGGACAACAAATCAACCAGAATCAGCAAAACAACATCTTCCGAGGGACACAGGGAAATCATATAAAAGCTGGTGGCGTTTACAATTCAAATTCTGACAGGAAAACTTCGCCAAGCAAAAGTTTTATAAAGCATCCAAAACGAGGGTTGCAGCCGCAAGGGGGATTTCAGAATTCTCAGTTTCATCATATCAAAAATGCAAAGGGAAAGTTTGGTAATAGGCAAAACAGAAAAGGACTAAGCAATGAGAAGGAAGGAAAATCTACTCTTGCCGGCTCTATGAATCAAGGCAGGGAAAGGAAAAGATCTCTTTCTTTGTCCTATACGGAACAAGAAGTTCAACTGTGGCGTGAAGGACGTAGGAAGAACTATCCATCAAACACCAACATAGAGAAGAAGCTAAGTGAAAAGACAATAGACTCGGAGGCCATTGAGAGGGAGGCCAAAGCACGAAGGGAGCAACTCAAGGAGATTCTTACAAAGCAGGCTGAGATGGGAGTTGAAGTTGCTGAGATACCATCTCACTACCTCTCAGATTCAGAAAAACAAGGGAATGGAAGAGAGGAGAATAAGTCATGGACTAACAATCGGAGATCACAAAACAAGTTCGTTAAGAGAGACAGATATGATAAGAAGGACCGGTTTGCAAAGAGGCAAAGATCAAGTCAGAAGGATTCATCTAATGACCCTTCATTCAGCAAAAGGGAGCCAACCTTACTGCAGAAGCTTCTTAGTTCAGATATCAAGAGAGATAAAAGTCGATTATGCCAGGTGTTTAGGTTCATGGTGACAAACTCTTTCTTTAAAGATTTTTCTGACAAACCTTTGAAATTTCCTACTGTAGTAGTTAAAGAAAGTGTGGTGGAGGAAGGTGTGGTTGAAGAGTTGTCTTCAGTTGCCGGGAAAGGTGCTTATGGGGGCCGTGACAAGTCCACGTTTGAAAAAGTTGGTAACAATGATTATAAAGATTGTTGTTACGATGATGATCATGTTGAAGATGAGGAAGAAGAGGGAGAGATCATCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko05164 map03015 map05164
Pfam Domains
Protein Families
NUFIP1

Protein Analysis

561

Amino Acids

62.51

Weight (kDa)

9.35

Isoelectric Point (pI)

53.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUFIP1 PF10453 324 - 356 1.8e-08 FMR1-interacting protein 1 (NUFIP1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 785, 1620
AciI CCGC 2 cut(s) 57, 812
AclWI GGATC 1 cut(s) 577
AcoI YGGCCR 2 cut(s) 37, 51
AcsI RAATTY 7 cut(s) 31, 127, 178, 436, 748, 829, 1487
AcuI CTGAAG 1 cut(s) 1533
AgeI ACCGGT 1 cut(s) 1296
AgsI TTSAA 9 cut(s) 436, 499, 747, 998, 1160, 1486, 1538, 1598, 1652
AjnI CCWGG 3 cut(s) 265, 406, 1425
AluBI AGCT 5 cut(s) 44, 72, 728, 1057, 1384
AluI AGCT 5 cut(s) 44, 72, 728, 1057, 1384
Alw21I GWGCWC 1 cut(s) 645
Alw26I GTCTC 1 cut(s) 1269
Alw44I GTGCAC 1 cut(s) 641
AlwI GGATC 1 cut(s) 577
AoxI GGCC 6 cut(s) 37, 51, 464, 1083, 1098, 1576
ApaLI GTGCAC 1 cut(s) 641
ApeKI GCWGC 5 cut(s) 94, 142, 326, 590, 808
ApoI RAATTY 7 cut(s) 31, 127, 178, 436, 748, 829, 1487
ArsI GACNNNNNNTTYG 2 cut(s) 1306, 1338
AsiGI ACCGGT 1 cut(s) 1296
AspS9I GGNCC 7 cut(s) 158, 251, 293, 464, 548, 1294, 1576
AsuC2I CCSGG 3 cut(s) 50, 168, 1558
AsuHPI GGTGA 3 cut(s) 17, 38, 1456
AvaII GGWCC 5 cut(s) 158, 251, 293, 548, 1294
AxyI CCTNAGG 1 cut(s) 585
BaeGI GKGCMC 2 cut(s) 228, 645
BbsI GAAGAC 1 cut(s) 1539
Bbv12I GWGCWC 1 cut(s) 645
BbvCI CCTCAGC 1 cut(s) 162
BbvI GCAGC 5 cut(s) 106, 129, 313, 577, 820
BccI CCATC 6 cut(s) 88, 124, 148, 1039, 1144, 1183
BceAI ACGGC 1 cut(s) 1563
BciT130I CCWGG 3 cut(s) 267, 408, 1427
BclI TGATCA 1 cut(s) 1642
BcnI CCSGG 3 cut(s) 50, 168, 1558
BcoDI GTCTC 1 cut(s) 1269
BfaI CTAG 1 cut(s) 1684
BfmI CTRYAG 3 cut(s) 591, 1376, 1497
BglI GCCNNNNNGGC 1 cut(s) 472
BglII AGATCT 1 cut(s) 962
BisI GCNGC 6 cut(s) 95, 143, 327, 591, 809, 812
BlsI GCNGC 6 cut(s) 96, 144, 328, 592, 810, 813
Bme1390I CCNGG 6 cut(s) 50, 168, 267, 408, 1427, 1558
Bme18I GGWCC 5 cut(s) 158, 251, 293, 548, 1294
BmgT120I GGNCC 7 cut(s) 158, 251, 293, 464, 548, 1294, 1576
BmiI GGNNCC 5 cut(s) 159, 244, 253, 1365, 1577
BmrFI CCNGG 6 cut(s) 50, 168, 267, 408, 1427, 1558
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 1 cut(s) 799
BmuI ACTGGG 1 cut(s) 270
BpiI GAAGAC 1 cut(s) 1539
Bpu10I CCTNAGC 1 cut(s) 162
BpuEI CTTGAG 1 cut(s) 1106
BpuMI CCSGG 3 cut(s) 50, 168, 1558
BsaBI GATNNNNATC 1 cut(s) 1641
BsaJI CCNNGG 3 cut(s) 247, 541, 700
BsaWI WCCGGW 1 cut(s) 1296
BsaXI ACNNNNNCTCC 4 cut(s) 1146, 1176, 1214, 1244
Bse118I RCCGGY 3 cut(s) 53, 929, 1296
Bse1I ACTGG 1 cut(s) 276
Bse21I CCTNAGG 1 cut(s) 585
Bse3DI GCAATG 1 cut(s) 907
Bse8I GATNNNNATC 1 cut(s) 1641
BseBI CCWGG 3 cut(s) 267, 408, 1427
BseDI CCNNGG 3 cut(s) 247, 541, 700
BseGI GGATG 1 cut(s) 790
BseJI GATNNNNATC 1 cut(s) 1641
BseMI GCAATG 1 cut(s) 907
BseMII CTCAG 6 cut(s) 176, 599, 848, 1137, 1158, 1203
BseNI ACTGG 1 cut(s) 276
BseRI GAGGAG 2 cut(s) 75, 1235
BseSI GKGCMC 2 cut(s) 228, 645
BseX3I CGGCCG 1 cut(s) 51
BseXI GCAGC 5 cut(s) 106, 129, 313, 577, 820
Bsh1285I CGRYCG 1 cut(s) 54
BshFI GGCC 6 cut(s) 39, 53, 466, 1085, 1100, 1578
BshTI ACCGGT 1 cut(s) 1296
BsiEI CGRYCG 1 cut(s) 54
BsiHKAI GWGCWC 1 cut(s) 645
BsiSI CCGG 7 cut(s) 36, 50, 54, 168, 930, 1297, 1557
BslFI GGGAC 4 cut(s) 171, 217, 237, 718
BsmAI GTCTC 1 cut(s) 1269
BsmFI GGGAC 4 cut(s) 171, 217, 237, 718
BsnI GGCC 6 cut(s) 39, 53, 466, 1085, 1100, 1578
Bsp1286I GDGCHC 2 cut(s) 228, 645
Bsp143I GATC 6 cut(s) 569, 962, 1250, 1316, 1642, 1674
BspACI CCGC 2 cut(s) 57, 812
BspANI GGCC 6 cut(s) 39, 53, 466, 1085, 1100, 1578
BspCNI CTCAG 6 cut(s) 175, 598, 847, 1138, 1159, 1202
BspHI TCATGA 1 cut(s) 174
BspLI GGNNCC 5 cut(s) 159, 244, 253, 1365, 1577
BspMAI CTGCAG 2 cut(s) 595, 1380
BspPI GGATC 1 cut(s) 577
BsrDI GCAATG 1 cut(s) 907
BsrFI RCCGGY 3 cut(s) 53, 929, 1296
BsrI ACTGG 1 cut(s) 276
BssAI RCCGGY 3 cut(s) 53, 929, 1296
BssECI CCNNGG 3 cut(s) 247, 541, 700
BssMI GATC 6 cut(s) 569, 962, 1250, 1316, 1642, 1674
BssT1I CCWWGG 2 cut(s) 247, 541
Bst2UI CCWGG 3 cut(s) 267, 408, 1427
Bst4CI ACNGT 2 cut(s) 1002, 1498
Bst6I CTCTTC 3 cut(s) 1210, 1533, 1659
BstAPI GCANNNNNTGC 1 cut(s) 103
BstC8I GCNNGC 3 cut(s) 55, 931, 1143
BstDEI CTNAG 9 cut(s) 162, 585, 834, 896, 1058, 1146, 1167, 1189, 1388
BstENI CCTNNNNNAGG 1 cut(s) 540
BstF5I GGATG 1 cut(s) 790
BstKTI GATC 6 cut(s) 572, 965, 1253, 1319, 1645, 1677
BstMAI GTCTC 1 cut(s) 1269
BstMBI GATC 6 cut(s) 569, 962, 1250, 1316, 1642, 1674
BstMCI CGRYCG 1 cut(s) 54
BstMWI GCNNNNNNNGC 5 cut(s) 50, 103, 472, 587, 617
BstNI CCWGG 3 cut(s) 267, 408, 1427
BstSCI CCNGG 6 cut(s) 48, 166, 265, 406, 1425, 1556
BstSFI CTRYAG 3 cut(s) 591, 1376, 1497
BstSLI GKGCMC 2 cut(s) 228, 645
BstV1I GCAGC 5 cut(s) 106, 129, 313, 577, 820
BstV2I GAAGAC 1 cut(s) 1539
BstX2I RGATCY 1 cut(s) 962
BstYI RGATCY 1 cut(s) 962
BstZI CGGCCG 1 cut(s) 51
Bsu36I CCTNAGG 1 cut(s) 585
BsuRI GGCC 6 cut(s) 39, 53, 466, 1085, 1100, 1578
BtsCI GGATG 1 cut(s) 790
BtsIMutI CAGTG 1 cut(s) 283
Cac8I GCNNGC 3 cut(s) 55, 931, 1143
CciI TCATGA 1 cut(s) 174
Cfr10I RCCGGY 3 cut(s) 53, 929, 1296
Cfr13I GGNCC 7 cut(s) 158, 251, 293, 464, 548, 1294, 1576
CsiI ACCWGGT 2 cut(s) 265, 406
CspAI ACCGGT 1 cut(s) 1296
CviAII CATG 7 cut(s) 132, 175, 229, 578, 1233, 1441, 1646
DdeI CTNAG 9 cut(s) 162, 585, 834, 896, 1058, 1146, 1167, 1189, 1388
DpnI GATC 6 cut(s) 571, 964, 1252, 1318, 1644, 1676
DpnII GATC 6 cut(s) 569, 962, 1250, 1316, 1642, 1674
DraI TTTAAA 1 cut(s) 1462
EaeI YGGCCR 2 cut(s) 37, 51
EagI CGGCCG 1 cut(s) 51
Eam1104I CTCTTC 3 cut(s) 1210, 1533, 1659
EarI CTCTTC 3 cut(s) 1210, 1533, 1659
EclXI CGGCCG 1 cut(s) 51
Eco130I CCWWGG 2 cut(s) 247, 541
Eco32I GATATC 1 cut(s) 1399
Eco47I GGWCC 5 cut(s) 158, 251, 293, 548, 1294
Eco52I CGGCCG 1 cut(s) 51
Eco57I CTGAAG 1 cut(s) 1533
Eco81I CCTNAGG 1 cut(s) 585
EcoNI CCTNNNNNAGG 1 cut(s) 540
EcoO109I RGGNCCY 1 cut(s) 158
EcoRI GAATTC 1 cut(s) 829
EcoRII CCWGG 3 cut(s) 265, 406, 1425
EcoRV GATATC 1 cut(s) 1399
EcoT14I CCWWGG 2 cut(s) 247, 541
EcoT22I ATGCAT 1 cut(s) 562
ErhI CCWWGG 2 cut(s) 247, 541
FaeI CATG 7 cut(s) 135, 178, 232, 581, 1236, 1444, 1649
FaqI GGGAC 4 cut(s) 171, 217, 237, 718
FatI CATG 7 cut(s) 131, 174, 228, 577, 1232, 1440, 1645
FauNDI CATATG 2 cut(s) 216, 469
FbaI TGATCA 1 cut(s) 1642
Fnu4HI GCNGC 6 cut(s) 95, 143, 327, 591, 809, 812
FokI GGATG 1 cut(s) 777
Fsp4HI GCNGC 6 cut(s) 95, 143, 327, 591, 809, 812
FspBI CTAG 1 cut(s) 1684
GluI GCNGC 6 cut(s) 95, 143, 327, 591, 809, 812
HaeIII GGCC 6 cut(s) 39, 53, 466, 1085, 1100, 1578
HapII CCGG 7 cut(s) 36, 50, 54, 168, 930, 1297, 1557
Hin1II CATG 7 cut(s) 135, 178, 232, 581, 1236, 1444, 1649
HindIII AAGCTT 1 cut(s) 1382
HpaII CCGG 7 cut(s) 36, 50, 54, 168, 930, 1297, 1557
HphI GGTGA 3 cut(s) 17, 38, 1456
Hpy166II GTNNAC 4 cut(s) 296, 643, 739, 1590
Hpy188III TCNNGA 2 cut(s) 175, 1402
Hpy8I GTNNAC 4 cut(s) 296, 643, 739, 1590
HpyCH4III ACNGT 2 cut(s) 1002, 1498
HpyCH4IV ACGT 2 cut(s) 1015, 1592
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 103, 472, 587, 617
HpyF3I CTNAG 9 cut(s) 162, 585, 834, 896, 1058, 1146, 1167, 1189, 1388
HpySE526I ACGT 2 cut(s) 1015, 1592
Hsp92II CATG 7 cut(s) 135, 178, 232, 581, 1236, 1444, 1649
KroI GCCGGC 2 cut(s) 53, 929
KroNI GCCGGC 2 cut(s) 55, 931
Ksp22I TGATCA 1 cut(s) 1642
Kzo9I GATC 6 cut(s) 569, 962, 1250, 1316, 1642, 1674
LmnI GCTCC 3 cut(s) 82, 1114, 1363
Lsp1109I GCAGC 5 cut(s) 106, 129, 313, 577, 820
LweI GCATC 1 cut(s) 799
MabI ACCWGGT 2 cut(s) 265, 406
MaeI CTAG 1 cut(s) 1684
MaeII ACGT 2 cut(s) 1015, 1592
MaeIII GTNAC 6 cut(s) 3, 209, 1444, 1580, 1607, 1631
MalI GATC 6 cut(s) 571, 964, 1252, 1318, 1644, 1676
MboI GATC 6 cut(s) 569, 962, 1250, 1316, 1642, 1674
MfeI CAATTG 1 cut(s) 413
MflI RGATCY 1 cut(s) 962
MhlI GDGCHC 2 cut(s) 228, 645
MlyI GAGTC 1 cut(s) 1070
MmeI TCCRAC 1 cut(s) 262
Mph1103I ATGCAT 1 cut(s) 562
MroNI GCCGGC 2 cut(s) 53, 929
MseI TTAA 5 cut(s) 111, 342, 1269, 1461, 1506
MslI CAYNNNNRTG 1 cut(s) 219
MspI CCGG 7 cut(s) 36, 50, 54, 168, 930, 1297, 1557
MspR9I CCNGG 6 cut(s) 50, 168, 267, 408, 1427, 1558
MunI CAATTG 1 cut(s) 413
MvaI CCWGG 3 cut(s) 267, 408, 1427
MwoI GCNNNNNNNGC 5 cut(s) 50, 103, 472, 587, 617
NaeI GCCGGC 2 cut(s) 55, 931
NciI CCSGG 3 cut(s) 50, 168, 1558
NdeI CATATG 2 cut(s) 216, 469
NdeII GATC 6 cut(s) 569, 962, 1250, 1316, 1642, 1674
NgoMIV GCCGGC 2 cut(s) 53, 929
NlaIII CATG 7 cut(s) 135, 178, 232, 581, 1236, 1444, 1649
NlaIV GGNNCC 5 cut(s) 159, 244, 253, 1365, 1577
NmuCI GTSAC 2 cut(s) 1444, 1580
NsiI ATGCAT 1 cut(s) 562
PagI TCATGA 1 cut(s) 174
PdiI GCCGGC 2 cut(s) 55, 931
PfeI GAWTC 9 cut(s) 370, 451, 490, 505, 679, 940, 1129, 1193, 1331
PinAI ACCGGT 1 cut(s) 1296
PkrI GCNGC 6 cut(s) 96, 144, 328, 592, 810, 813
PleI GAGTC 1 cut(s) 1070
PpsI GAGTC 1 cut(s) 1070
PpuMI RGGWCCY 1 cut(s) 158
PsiI TTATAA 2 cut(s) 785, 1620
Psp5II RGGWCCY 1 cut(s) 158
Psp6I CCWGG 3 cut(s) 265, 406, 1425
PspGI CCWGG 3 cut(s) 265, 406, 1425
PspN4I GGNNCC 5 cut(s) 159, 244, 253, 1365, 1577
PspPI GGNCC 7 cut(s) 158, 251, 293, 464, 548, 1294, 1576
PspPPI RGGWCCY 1 cut(s) 158
PstI CTGCAG 2 cut(s) 595, 1380
PsuI RGATCY 1 cut(s) 962
RseI CAYNNNNRTG 1 cut(s) 219
SaqAI TTAA 5 cut(s) 111, 342, 1269, 1461, 1506
SatI GCNGC 6 cut(s) 95, 143, 327, 591, 809, 812
Sau3AI GATC 6 cut(s) 569, 962, 1250, 1316, 1642, 1674
Sau96I GGNCC 7 cut(s) 158, 251, 293, 464, 548, 1294, 1576
SchI GAGTC 1 cut(s) 1070
ScrFI CCNGG 6 cut(s) 50, 168, 267, 408, 1427, 1558
SduI GDGCHC 2 cut(s) 228, 645
SexAI ACCWGGT 2 cut(s) 265, 406
SfaNI GCATC 1 cut(s) 799
SfcI CTRYAG 3 cut(s) 591, 1376, 1497
SinI GGWCC 5 cut(s) 158, 251, 293, 548, 1294
SmiMI CAYNNNNRTG 1 cut(s) 219
SmlI CTYRAG 1 cut(s) 1121
SmoI CTYRAG 1 cut(s) 1121
SsiI CCGC 2 cut(s) 57, 812
SspI AATATT 1 cut(s) 460
SspMI CTAG 1 cut(s) 1684
StyD4I CCNGG 6 cut(s) 48, 166, 265, 406, 1425, 1556
StyI CCWWGG 2 cut(s) 247, 541
TaaI ACNGT 2 cut(s) 1002, 1498
TaiI ACGT 2 cut(s) 1018, 1595
TaqI TCGA 2 cut(s) 152, 1417
TauI GCSGC 1 cut(s) 814
TfiI GAWTC 9 cut(s) 370, 451, 490, 505, 679, 940, 1129, 1193, 1331
Tru1I TTAA 5 cut(s) 111, 342, 1269, 1461, 1506
Tru9I TTAA 5 cut(s) 111, 342, 1269, 1461, 1506
TscAI CASTG 1 cut(s) 283
TseFI GTSAC 2 cut(s) 1444, 1580
TseI GCWGC 5 cut(s) 94, 142, 326, 590, 808
Tsp45I GTSAC 2 cut(s) 1444, 1580
TspGWI ACGGA 1 cut(s) 998
TspRI CASTG 1 cut(s) 283
VneI GTGCAC 1 cut(s) 641
VpaK11BI GGWCC 5 cut(s) 158, 251, 293, 548, 1294
XagI CCTNNNNNAGG 1 cut(s) 540
XapI RAATTY 7 cut(s) 31, 127, 178, 436, 748, 829, 1487
XspI CTAG 1 cut(s) 1684
Zsp2I ATGCAT 1 cut(s) 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.