Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0005893

Physical Location & Seq

Genomic Coordinates Forward (+)

3676296 .. 3681130

Gene Structure 4835 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom07g04250.8

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1689 bp

ATGTTACCTCACTACAATTCCTTTCCTCGCCATTCCAACCGAGCTCACACAACTGGTAGCTTGGCTCCACCTCCTCACCAGATGCAACCTCAATTGGGTATCAGGAATCTCGAAATTCCAGTCCAATTTAGTAATTCTAATTCCATGATAGCTCAACCCAATTTCATGAATCTACCAAACAACCTTCTTCCTATGCAAAATAACCATCTGGGTATGCCTCAATTCGGTTCCGTTGGCCCTACTTCTCAACCGGCTCAGCCGAATGTTGGGGTTTTCGGTTCGCAAGGTATGCCTCAATTCTGCAATTTGGTGCAGCAAGTGAATCAATTGATGTCATCTCAAGTGCCTGGGCAAATATTTGGGCATAACATGTTAAATTTACAGCAACAGATGAACCAGAATATGGGTTTGCCGTATGGACAATTTTGTTTGCCGAATTCAATGCAAAACATGAATCAATTTGTGCCAATGCAAATGCAAAATCCATCGCAAGTCCATCCTAATTATGGTTTTCCGGGATCAAATCAAGCACCTCAGACCACAATGTCTCAAAATGTCCCATTGTTTGCAAATAACCAGGTTAATCTGCATTCTAATCAGGCAGGCCAGCAAGTTAACCAAAATCAGCAAAACTTGGGTCTACCTCCAGTGCAAGGGACACAGGGAAATAAAACAAATAGTAGTGGTGGTTACAATTCAAATTCCAATTGGAAACAATCACCAAGCAAAAGCGTTACAAAGAATCCGAAGAATCACCAAGGGGGATTTCAGAATCCTCAGTCGCATCATATGAAAAATGCAAAGGGAAAGTTCGCATTTCCTAATGGGCACAAAGGAAAAGGGTTGAGAAATGAAAGCGAAGGAAAGTTTGCTCTGGCCAACTCTTCAAACCAGGGCAGGGAAAGGAAGAGACCTCTTTTCTTGTCATATACGGAACAAGAGATTGAGCGGTGGCGGGAAGACCGTAGGAAGCACTACCCGTCGAAATCCAACATAGAGAAGAAGATAAGTGAAAAGCAGATAAATGTAGAGGTCATCGAAAGAGAGGCAAACATTCGACGAGAGCAACTCAAGGATATTCTTACAAAGCAGGCTGAGTTGGGAGTTCAAGTTGCTGAAGTACCATCGCACTACCTCGCAGATTCAAATCAAGGGCATATAAAGGAAGATAATGAAACATTGACCAAGAAGGGGAGGTCAACAAACAAGTTTGGTAAGAGAGAAAAATATGATAGAAAGGATCGGTTTTCCAAGAGGCAAAGATCACATCAAAAGGATTCGTCTAATGACCCTTCTTTCGACAAAAGGGAGCCAAACAAAAGGGAGCCAACCTTGCTGCAGAAGCTTCTTAGTGCAGATATTAGGAGAGACAGAAGCCACCTGCTGCAGGTGTTTAGGTTCATGGTGACAAACTCTTTCTTCAAAGATTGTCCTGACAAACCTTTGAAATTCCCTACTGTAGCAGTAAAAGAAAGTGCCGGTGACGGAGAGGTGGCTCAAAAGCCGTCCTCACTTATAGAGAAAGATGCTTTTAAGGGAAGTGATAAATCCATGGTTGGAAGAGTTTATAACAATCATTCTGGAAATAACAACGATGTCGAGGATTCTGTTGATAGCAGTGATGAAGATGACGGTGAAGGAGAGGGAACAGAGAGACCTGCGGAAGAAGAGGAAGAAATCATAGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko03015 ko05164 map03015 map05164

Pfam Domains

Protein Families

NUFIP1

Protein Analysis

563

Amino Acids

63.33

Weight (kDa)

9.24

Isoelectric Point (pI)

56.86

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005893)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G18440 AT5G18440
fragaria_vesca	FvH4_1g30390 FvH4_1g30390 FvH4_1g30390 FvH4_1g30390
malus_domestica	MD02G1263300.v1.1 MD07G1054700.v1.1
prunus_persica	Prupe.2G061400_v2.0.a1 Prupe.2G061400_v2.0.a1 Prupe.2G061400_v2.0.a1 Prupe.2G061400_v2.0.a1
pyrus_communis	pycom02g22570 pycom07g04250
rosa_chinensis	RchiOBHm_Chr3g0490591
rosa_laevigata	RLG00000022881
rosa_multiflora	Rmu_sc0007326.1_g000001 Rmu_sc0035249.1_g000001
rosa_roxburghii	Rroxscaffold_6G00393380
rosa_rugosa	Rorug03G0244300 Rorug03G0244400 Rorug03G0244500 Rorug03G0244600
rosa_samantha	Rh3AG294000 Rh3BG330200 Rh3CG327600 Rh3DG326900
rosa_wichuraiana	Rw3G026000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1569
AarI	CACCTGC	2 cut(s)	1378, 1389
AasI	GACNNNNNNGTC	1 cut(s)	544
Acc36I	ACCTGC	3 cut(s)	1378, 1389, 1666
AccB7I	CCANNNNNTGG	1 cut(s)	403
AccBSI	CCGCTC	1 cut(s)	949
AccI	GTMKAC	1 cut(s)	640
AciI	CCGC	3 cut(s)	949, 955, 1661
AclWI	GGATC	2 cut(s)	526, 1248
AcoI	YGGCCR	1 cut(s)	876
AcsI	RAATTY	5 cut(s)	114, 376, 436, 700, 1448
AcuI	CTGAAG	1 cut(s)	1137
AfaI	GTAC	1 cut(s)	1122
AfiI	CCNNNNNNNGG	9 cut(s)	95, 224, 266, 403, 506, 653, 898, 1191, 1387
AflIII	ACRYGT	1 cut(s)	369
AgsI	TTSAA	7 cut(s)	441, 699, 888, 1109, 1146, 1423, 1447
AjnI	CCWGG	3 cut(s)	346, 576, 891
AjuI	GAANNNNNNNTTGG	2 cut(s)	750, 782
AluBI	AGCT	4 cut(s)	44, 60, 152, 1345
AluI	AGCT	4 cut(s)	44, 60, 152, 1345
Alw21I	GWGCWC	1 cut(s)	46
Alw26I	GTCTC	4 cut(s)	552, 904, 1362, 1648
AlwI	GGATC	2 cut(s)	526, 1248
AoxI	GGCC	3 cut(s)	235, 604, 876
ApeKI	GCWGC	3 cut(s)	313, 1336, 1384
ApoI	RAATTY	5 cut(s)	114, 376, 436, 700, 1448
ArsI	GACNNNNNNTTYG	6 cut(s)	105, 137, 622, 654, 1280, 1312
AspS9I	GGNCC	1 cut(s)	236
AsuC2I	CCSGG	1 cut(s)	516
AsuHPI	GGTGA	6 cut(s)	68, 711, 746, 1417, 1493, 1646
BaeGI	GKGCMC	1 cut(s)	831
BalI	TGGCCA	1 cut(s)	878
BanII	GRGCYC	1 cut(s)	46
BbsI	GAAGAC	1 cut(s)	966
Bbv12I	GWGCWC	1 cut(s)	46
BbvI	GCAGC	3 cut(s)	325, 1323, 1371
BccI	CCATC	4 cut(s)	213, 493, 504, 1132
BceAI	ACGGC	2 cut(s)	397, 1489
BcgI	CGANNNNNNTGC	2 cut(s)	424, 458
BciT130I	CCWGG	3 cut(s)	348, 578, 893
BcnI	CCSGG	1 cut(s)	516
BcoDI	GTCTC	4 cut(s)	552, 904, 1362, 1648
BfaI	CTAG	1 cut(s)	1687
BfmI	CTRYAG	3 cut(s)	1337, 1385, 1458
BfuAI	ACCTGC	3 cut(s)	1378, 1389, 1666
BisI	GCNGC	3 cut(s)	314, 1337, 1385
BlpI	GCTNAGC	1 cut(s)	255
BlsI	GCNGC	3 cut(s)	315, 1338, 1386
Bme1390I	CCNGG	4 cut(s)	348, 516, 578, 893
BmgT120I	GGNCC	1 cut(s)	236
BmiI	GGNNCC	4 cut(s)	66, 229, 1311, 1326
BmrFI	CCNGG	4 cut(s)	348, 516, 578, 893
BmsI	GCATC	3 cut(s)	72, 793, 1516
BpiI	GAAGAC	1 cut(s)	966
BplI	GAGNNNNNCTC	2 cut(s)	1053, 1085
BpmI	CTGGAG	1 cut(s)	630
Bpu1102I	GCTNAGC	1 cut(s)	255
BpuEI	CTTGAG	2 cut(s)	324, 1055
BpuMI	CCSGG	1 cut(s)	516
BsaBI	GATNNNNATC	1 cut(s)	1146
BsaI	GGTCTC	2 cut(s)	904, 1648
BsaJI	CCNNGG	4 cut(s)	347, 757, 892, 1551
BsaXI	ACNNNNNCTCC	2 cut(s)	1095, 1125
Bsc4I	CCNNNNNNNGG	9 cut(s)	95, 224, 266, 403, 506, 653, 898, 1191, 1387
Bse118I	RCCGGY	2 cut(s)	250, 1478
Bse1I	ACTGG	3 cut(s)	58, 119, 647
Bse8I	GATNNNNATC	1 cut(s)	1146
BseBI	CCWGG	3 cut(s)	348, 578, 893
BseDI	CCNNGG	4 cut(s)	347, 757, 892, 1551
BseGI	GGATG	1 cut(s)	496
BseJI	GATNNNNATC	1 cut(s)	1146
BseLI	CCNNNNNNNGG	9 cut(s)	95, 224, 266, 403, 506, 653, 898, 1191, 1387
BseMII	CTCAG	4 cut(s)	269, 548, 791, 1086
BseNI	ACTGG	3 cut(s)	58, 119, 647
BseRI	GAGGAG	1 cut(s)	63
BseSI	GKGCMC	1 cut(s)	831
BseXI	GCAGC	3 cut(s)	325, 1323, 1371
BsgI	GTGCAG	2 cut(s)	332, 1374
BshFI	GGCC	3 cut(s)	237, 606, 878
BsiHKAI	GWGCWC	1 cut(s)	46
BsiSI	CCGG	3 cut(s)	251, 515, 1479
BslFI	GGGAC	2 cut(s)	542, 670
BslI	CCNNNNNNNGG	9 cut(s)	95, 224, 266, 403, 506, 653, 898, 1191, 1387
BsmAI	GTCTC	4 cut(s)	552, 904, 1362, 1648
BsmFI	GGGAC	2 cut(s)	542, 670
BsmI	GAATGC	1 cut(s)	589
BsnI	GGCC	3 cut(s)	237, 606, 878
Bso31I	GGTCTC	2 cut(s)	904, 1648
Bsp1286I	GDGCHC	2 cut(s)	46, 831
Bsp143I	GATC	3 cut(s)	518, 1240, 1262
Bsp1720I	GCTNAGC	1 cut(s)	255
Bsp19I	CCATGG	1 cut(s)	1551
BspACI	CCGC	3 cut(s)	949, 955, 1661
BspANI	GGCC	3 cut(s)	237, 606, 878
BspCNI	CTCAG	4 cut(s)	268, 547, 790, 1087
BspHI	TCATGA	1 cut(s)	165
BspLI	GGNNCC	4 cut(s)	66, 229, 1311, 1326
BspMAI	CTGCAG	2 cut(s)	1341, 1389
BspMI	ACCTGC	3 cut(s)	1378, 1389, 1666
BspPI	GGATC	2 cut(s)	526, 1248
BspTNI	GGTCTC	2 cut(s)	904, 1648
BsrBI	CCGCTC	1 cut(s)	949
BsrFI	RCCGGY	2 cut(s)	250, 1478
BsrI	ACTGG	3 cut(s)	58, 119, 647
BssAI	RCCGGY	2 cut(s)	250, 1478
BssECI	CCNNGG	4 cut(s)	347, 757, 892, 1551
BssMI	GATC	3 cut(s)	518, 1240, 1262
BssT1I	CCWWGG	2 cut(s)	757, 1551
Bst2UI	CCWGG	3 cut(s)	348, 578, 893
Bst4CI	ACNGT	3 cut(s)	965, 1459, 1634
Bst6I	CTCTTC	4 cut(s)	889, 902, 1555, 1662
BstAPI	GCANNNNNTGC	1 cut(s)	289
BstC8I	GCNNGC	3 cut(s)	604, 608, 1092
BstDEI	CTNAG	5 cut(s)	255, 534, 777, 1095, 1349
BstDSI	CCRYGG	1 cut(s)	1551
BstENI	CCTNNNNNAGG	1 cut(s)	1385
BstF5I	GGATG	1 cut(s)	496
BstKTI	GATC	3 cut(s)	521, 1243, 1265
BstMAI	GTCTC	4 cut(s)	552, 904, 1362, 1648
BstMBI	GATC	3 cut(s)	518, 1240, 1262
BstMWI	GCNNNNNNNGC	3 cut(s)	289, 1333, 1342
BstNI	CCWGG	3 cut(s)	348, 578, 893
BstNSI	RCATGY	1 cut(s)	373
BstSCI	CCNGG	4 cut(s)	346, 514, 576, 891
BstSFI	CTRYAG	3 cut(s)	1337, 1385, 1458
BstSLI	GKGCMC	1 cut(s)	831
BstV1I	GCAGC	3 cut(s)	325, 1323, 1371
BstV2I	GAAGAC	1 cut(s)	966
BsuRI	GGCC	3 cut(s)	237, 606, 878
BtgI	CCRYGG	1 cut(s)	1551
BtgZI	GCGATG	2 cut(s)	471, 1110
BtsCI	GGATG	1 cut(s)	496
BtsI	GCAGTG	1 cut(s)	1624
BtsIMutI	CAGTG	2 cut(s)	654, 1624
BveI	ACCTGC	3 cut(s)	1378, 1389, 1666
Cac8I	GCNNGC	3 cut(s)	604, 608, 1092
CciI	TCATGA	1 cut(s)	165
Cfr10I	RCCGGY	2 cut(s)	250, 1478
Cfr13I	GGNCC	1 cut(s)	236
CsiI	ACCWGGT	1 cut(s)	576
Csp6I	GTAC	1 cut(s)	1121
CviAII	CATG	6 cut(s)	145, 166, 370, 451, 1402, 1552
CviQI	GTAC	1 cut(s)	1121
DdeI	CTNAG	5 cut(s)	255, 534, 777, 1095, 1349
DpnI	GATC	3 cut(s)	520, 1242, 1264
DpnII	GATC	3 cut(s)	518, 1240, 1262
DrdI	GACNNNNNNGTC	1 cut(s)	544
DseDI	GACNNNNNNGTC	1 cut(s)	544
EaeI	YGGCCR	1 cut(s)	876
Eam1104I	CTCTTC	4 cut(s)	889, 902, 1555, 1662
EarI	CTCTTC	4 cut(s)	889, 902, 1555, 1662
Ecl136II	GAGCTC	1 cut(s)	44
Eco130I	CCWWGG	2 cut(s)	757, 1551
Eco24I	GRGCYC	1 cut(s)	46
Eco31I	GGTCTC	2 cut(s)	904, 1648
Eco53kI	GAGCTC	1 cut(s)	44
Eco57I	CTGAAG	1 cut(s)	1137
EcoICRI	GAGCTC	1 cut(s)	44
EcoNI	CCTNNNNNAGG	1 cut(s)	1385
EcoRI	GAATTC	1 cut(s)	436
EcoRII	CCWGG	3 cut(s)	346, 576, 891
EcoT14I	CCWWGG	2 cut(s)	757, 1551
EcoT38I	GRGCYC	1 cut(s)	46
ErhI	CCWWGG	2 cut(s)	757, 1551
FaeI	CATG	6 cut(s)	148, 169, 373, 454, 1405, 1555
FaqI	GGGAC	2 cut(s)	542, 670
FatI	CATG	6 cut(s)	144, 165, 369, 450, 1401, 1551
FauI	CCCGC	1 cut(s)	948
FauNDI	CATATG	1 cut(s)	789
FblI	GTMKAC	1 cut(s)	640
Fnu4HI	GCNGC	3 cut(s)	314, 1337, 1385
FokI	GGATG	1 cut(s)	483
FriOI	GRGCYC	1 cut(s)	46
Fsp4HI	GCNGC	3 cut(s)	314, 1337, 1385
FspBI	CTAG	1 cut(s)	1687
GluI	GCNGC	3 cut(s)	314, 1337, 1385
GsuI	CTGGAG	1 cut(s)	630
HaeIII	GGCC	3 cut(s)	237, 606, 878
HapII	CCGG	3 cut(s)	251, 515, 1479
Hin1II	CATG	6 cut(s)	148, 169, 373, 454, 1405, 1555
HincII	GTYRAC	2 cut(s)	616, 1200
HindII	GTYRAC	2 cut(s)	616, 1200
HindIII	AAGCTT	1 cut(s)	1343
HpaI	GTTAAC	1 cut(s)	616
HpaII	CCGG	3 cut(s)	251, 515, 1479
HphI	GGTGA	6 cut(s)	68, 711, 746, 1417, 1493, 1646
Hpy166II	GTNNAC	3 cut(s)	616, 641, 1200
Hpy188I	TCNGA	3 cut(s)	537, 747, 771
Hpy188III	TCNNGA	5 cut(s)	103, 110, 166, 1433, 1581
Hpy8I	GTNNAC	3 cut(s)	616, 641, 1200
Hpy99I	CGWCG	2 cut(s)	985, 1062
HpyAV	CCTTC	5 cut(s)	194, 854, 1183, 1302, 1631
HpyCH4III	ACNGT	3 cut(s)	965, 1459, 1634
HpyF10VI	GCNNNNNNNGC	3 cut(s)	289, 1333, 1342
HpyF3I	CTNAG	5 cut(s)	255, 534, 777, 1095, 1349
Hsp92II	CATG	6 cut(s)	148, 169, 373, 454, 1405, 1555
KspAI	GTTAAC	1 cut(s)	616
Kzo9I	GATC	3 cut(s)	518, 1240, 1262
LmnI	GCTCC	3 cut(s)	70, 1309, 1324
Lsp1109I	GCAGC	3 cut(s)	325, 1323, 1371
LweI	GCATC	3 cut(s)	72, 793, 1516
MabI	ACCWGGT	1 cut(s)	576
MaeI	CTAG	1 cut(s)	1687
MaeIII	GTNAC	5 cut(s)	3, 689, 733, 1405, 1481
MalI	GATC	3 cut(s)	520, 1242, 1264
MbiI	CCGCTC	1 cut(s)	949
MboI	GATC	3 cut(s)	518, 1240, 1262
MfeI	CAATTG	3 cut(s)	92, 326, 706
MhlI	GDGCHC	2 cut(s)	46, 831
MlsI	TGGCCA	1 cut(s)	878
MluNI	TGGCCA	1 cut(s)	878
MmeI	TCCRAC	3 cut(s)	60, 1014, 1537
Mox20I	TGGCCA	1 cut(s)	878
MscI	TGGCCA	1 cut(s)	878
MseI	TTAA	4 cut(s)	374, 582, 615, 1533
Msp20I	TGGCCA	1 cut(s)	878
MspI	CCGG	3 cut(s)	251, 515, 1479
MspR9I	CCNGG	4 cut(s)	348, 516, 578, 893
MunI	CAATTG	3 cut(s)	92, 326, 706
Mva1269I	GAATGC	1 cut(s)	589
MvaI	CCWGG	3 cut(s)	348, 578, 893
MwoI	GCNNNNNNNGC	3 cut(s)	289, 1333, 1342
NciI	CCSGG	1 cut(s)	516
NcoI	CCATGG	1 cut(s)	1551
NdeI	CATATG	1 cut(s)	789
NdeII	GATC	3 cut(s)	518, 1240, 1262
NlaIII	CATG	6 cut(s)	148, 169, 373, 454, 1405, 1555
NlaIV	GGNNCC	4 cut(s)	66, 229, 1311, 1326
NmuCI	GTSAC	2 cut(s)	1405, 1481
NspI	RCATGY	1 cut(s)	373
PagI	TCATGA	1 cut(s)	165
PaqCI	CACCTGC	2 cut(s)	1378, 1389
PciI	ACATGT	1 cut(s)	369
PctI	GAATGC	1 cut(s)	589
PflMI	CCANNNNNTGG	1 cut(s)	403
PfoI	TCCNGGA	1 cut(s)	514
PkrI	GCNGC	3 cut(s)	315, 1338, 1386
PscI	ACATGT	1 cut(s)	369
PsiI	TTATAA	1 cut(s)	1569
Psp124BI	GAGCTC	1 cut(s)	46
Psp6I	CCWGG	3 cut(s)	346, 576, 891
PspGI	CCWGG	3 cut(s)	346, 576, 891
PspN4I	GGNNCC	4 cut(s)	66, 229, 1311, 1326
PspPI	GGNCC	1 cut(s)	236
PstI	CTGCAG	2 cut(s)	1341, 1389
RsaI	GTAC	1 cut(s)	1122
RsaNI	GTAC	1 cut(s)	1121
SacI	GAGCTC	1 cut(s)	46
SaqAI	TTAA	4 cut(s)	374, 582, 615, 1533
SatI	GCNGC	3 cut(s)	314, 1337, 1385
Sau3AI	GATC	3 cut(s)	518, 1240, 1262
Sau96I	GGNCC	1 cut(s)	236
ScrFI	CCNGG	4 cut(s)	348, 516, 578, 893
SduI	GDGCHC	2 cut(s)	46, 831
SexAI	ACCWGGT	1 cut(s)	576
SfaNI	GCATC	3 cut(s)	72, 793, 1516
SfcI	CTRYAG	3 cut(s)	1337, 1385, 1458
SmlI	CTYRAG	2 cut(s)	339, 1070
SmoI	CTYRAG	2 cut(s)	339, 1070
SsiI	CCGC	3 cut(s)	949, 955, 1661
SspI	AATATT	1 cut(s)	357
SspMI	CTAG	1 cut(s)	1687
SstI	GAGCTC	1 cut(s)	46
StyD4I	CCNGG	4 cut(s)	346, 514, 576, 891
StyI	CCWWGG	2 cut(s)	757, 1551
TaaI	ACNGT	3 cut(s)	965, 1459, 1634
TaqI	TCGA	6 cut(s)	111, 983, 1038, 1057, 1299, 1599
Tru1I	TTAA	4 cut(s)	374, 582, 615, 1533
Tru9I	TTAA	4 cut(s)	374, 582, 615, 1533
TscAI	CASTG	2 cut(s)	654, 1624
TseFI	GTSAC	2 cut(s)	1405, 1481
TseI	GCWGC	3 cut(s)	313, 1336, 1384
Tsp45I	GTSAC	2 cut(s)	1405, 1481
TspDTI	ATGAA	9 cut(s)	154, 182, 407, 467, 806, 867, 1188, 1390, 1638
TspGWI	ACGGA	3 cut(s)	220, 947, 1500
TspRI	CASTG	2 cut(s)	654, 1624
Van91I	CCANNNNNTGG	1 cut(s)	403
XagI	CCTNNNNNAGG	1 cut(s)	1385
XapI	RAATTY	5 cut(s)	114, 376, 436, 700, 1448
XceI	RCATGY	1 cut(s)	373
XcmI	CCANNNNNNNNNTGG	1 cut(s)	503
XmiI	GTMKAC	1 cut(s)	640
XspI	CTAG	1 cut(s)	1687

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.