AT5G20310

BEST Arabidopsis thaliana protein match is ATP binding kinase protein kinase protein serine threonine kinase (TAIR AT2G24370.1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0014277
Physical Location & Seq
Forward (+)
6857553 .. 6859145
1593 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G20310.1

Sequence Viewer

Length: 1185 bp
ATGGAGAGAAATGAAGTAAGGGAAGGGCCTATAGCTATTGCAATTGACAGAGATAAAACAACAAGCTGCCAAGCTCTTAAATGGGCTGTCGATCATTATATACCTCGAGGCGGGACTGTAAAACTCGTTCATGTCATCCAAAGATCTGCTTTGAATATTTCTAATGGATCTCACACAGATGATGAATCATCTGAAAGACAAAGCAATGATAAACGGACGACCGCTCAGTTTCTTCCATTGCGCTGTCTTTGTATGCGAAGAAATATACAAAGCGAAATAGTTTTGCTGGAAGATCAAGATGTAGCGAAAGCCTTGATCGAATATATTAGCCAGAATTGTATTTCCACATTCATTCTAGGTGCCTCGTTGAAGAAGAGCATTACCAGGCTGTTCAAGGCTGATGACATTCCAAGTAATGTGATGAGATGGGCTCCAGACTTCTGCACTGTATTAGTTTTATCAAAGGGAAGACTTTCAAGTGTACGGGCAGCCACTCGGCCTTTACCTCAAGTCTTGCCATCTTCTTCTTCAGGGACTGCACCTTTGTCACCACGCAGCAACGGCGATGAGGCTCCCTCTGAGATGTCATTGTCAAGAGAAGACGACGTTTTCCTTGAAGAGTTTTCATCCGTTGACTCAGATTCTAGTGTGAACATTAGTAACAGGTTCAGTACAGACAGTTCGGTTCTCGCCTTCTATGAGAAACTTGCAGCTCCACCCATGTTGGAGATTCCTAGATTCTCTGTTCTTGATGATGAGAAAGCCAAGTTTTCCATCTATCTTAATAGTCTTTCTGATGAAAAGAATTGTACATTAGCTTCCCCATTATCACCAGTCGATGACGCTACGGCTGAGATGAGAAGGCTGAAGAAAGAGCTCAAGGAAACGATGAACATGTACCACGCCGCTTGCAAAGAAGCTCTTATGGAAAAAGAAAGAGCAGTAGAGCTTGTGATGTGGAAAAAGAAAGCAGAACTAAGGATTCAAATGGCTGAAGAAACAGCAAGAATGGCTATCATGGAGATGAAGGCGGTGAAAGGGAAAGGGAGTGATGACAGAAAGATGGTATGGGATACTCTTGGAGAGTCTCATACTGTGGTTAAACATGAGAGCTTGCTCCATATTTTAGTTGTGCTTTTCTTGTTTTATATTTATTTCACACTAAGAAAACTCTATTTCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

394

Amino Acids

44.46

Weight (kDa)

6.43

Isoelectric Point (pI)

52.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014277)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20310
malus_domestica MD13G1232700.v1.1 MD13G1233300.v1.1 MD16G1238300.v1.1
prunus_persica Prupe.1G064200_v2.0.a1
pyrus_communis pycom13g20550
rosa_chinensis RchiOBHm_Chr4g0397631
rosa_laevigata RLG00000009422
rosa_multiflora Rmu_co8218642.1_g000001 Rmu_sc0002729.1_g000005
rosa_roxburghii Rroxscaffold_5G00342440
rosa_rugosa Rorug03G0342200
rosa_samantha Rh4AG079500 Rh4BG077500 Rh4CG085400 Rh4DG072500
rosa_wichuraiana Rw4G006510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 105
AccB1I GGYRCC 1 cut(s) 359
AccBSI CCGCTC 1 cut(s) 224
AciI CCGC 4 cut(s) 111, 222, 906, 1031
AclWI GGATC 1 cut(s) 175
AcuI CTGAAG 3 cut(s) 513, 887, 1014
AfaI GTAC 4 cut(s) 483, 673, 811, 899
AfiI CCNNNNNNNGG 1 cut(s) 110
AflIII ACRYGT 1 cut(s) 894
AgsI TTSAA 6 cut(s) 154, 370, 394, 477, 617, 986
AjnI CCWGG 1 cut(s) 383
AluBI AGCT 9 cut(s) 35, 66, 74, 713, 818, 877, 920, 949, 1113
AluI AGCT 9 cut(s) 35, 66, 74, 713, 818, 877, 920, 949, 1113
Alw21I GWGCWC 1 cut(s) 879
Alw26I GTCTC 1 cut(s) 1092
AlwI GGATC 1 cut(s) 175
AlwNI CAGNNNCTG 1 cut(s) 536
Ama87I CYCGRG 1 cut(s) 105
AoxI GGCC 2 cut(s) 26, 497
ApeKI GCWGC 4 cut(s) 66, 488, 555, 710
ArsI GACNNNNNNTTYG 2 cut(s) 526, 558
Asp700I GAANNNNTTC 1 cut(s) 472
AspLEI GCGC 1 cut(s) 243
AspS9I GGNCC 1 cut(s) 26
AsuHPI GGTGA 3 cut(s) 540, 822, 1045
AvaI CYCGRG 1 cut(s) 105
BanI GGYRCC 1 cut(s) 359
BanII GRGCYC 2 cut(s) 433, 879
BarI GAAGNNNNNNTAC 2 cut(s) 802, 834
BbsI GAAGAC 2 cut(s) 475, 606
Bbv12I GWGCWC 1 cut(s) 879
BbvI GCAGC 4 cut(s) 53, 500, 567, 722
BccI CCATC 4 cut(s) 420, 526, 782, 1057
BceAI ACGGC 2 cut(s) 577, 864
BciT130I CCWGG 1 cut(s) 385
BciVI GTATCC 1 cut(s) 1066
BcoDI GTCTC 1 cut(s) 1092
BfaI CTAG 3 cut(s) 356, 645, 735
BfmI CTRYAG 1 cut(s) 30
BfuI GTATCC 1 cut(s) 1066
BglII AGATCT 1 cut(s) 143
BisI GCNGC 5 cut(s) 67, 489, 556, 711, 906
BlsI GCNGC 5 cut(s) 68, 490, 557, 712, 907
Bme1390I CCNGG 1 cut(s) 385
BmeT110I CYCGRG 1 cut(s) 105
BmgT120I GGNCC 1 cut(s) 26
BmiI GGNNCC 3 cut(s) 361, 432, 573
BmrFI CCNGG 1 cut(s) 385
BpiI GAAGAC 2 cut(s) 475, 606
BplI GAGNNNNNCTC 4 cut(s) 415, 447, 560, 592
BpmI CTGGAG 1 cut(s) 417
BpuEI CTTGAG 2 cut(s) 492, 863
Bsc4I CCNNNNNNNGG 1 cut(s) 110
Bse1I ACTGG 1 cut(s) 833
Bse3DI GCAATG 2 cut(s) 211, 236
BseBI CCWGG 1 cut(s) 385
BseGI GGATG 2 cut(s) 135, 626
BseLI CCNNNNNNNGG 1 cut(s) 110
BseMI GCAATG 2 cut(s) 211, 236
BseMII CTCAG 4 cut(s) 239, 570, 651, 843
BseNI ACTGG 1 cut(s) 833
BseXI GCAGC 4 cut(s) 53, 500, 567, 722
BsgI GTGCAG 2 cut(s) 427, 522
Bsh1285I CGRYCG 1 cut(s) 222
BshFI GGCC 2 cut(s) 28, 499
BshNI GGYRCC 1 cut(s) 359
BsiEI CGRYCG 1 cut(s) 222
BsiHKAI GWGCWC 1 cut(s) 879
BsiHKCI CYCGRG 1 cut(s) 105
BslFI GGGAC 2 cut(s) 127, 547
BslI CCNNNNNNNGG 1 cut(s) 110
BsmAI GTCTC 1 cut(s) 1092
BsmFI GGGAC 2 cut(s) 127, 547
BsnI GGCC 2 cut(s) 28, 499
BsoBI CYCGRG 1 cut(s) 105
Bsp1286I GDGCHC 2 cut(s) 433, 879
Bsp1407I TGTACA 1 cut(s) 809
Bsp143I GATC 5 cut(s) 91, 143, 167, 292, 315
BspACI CCGC 4 cut(s) 111, 222, 906, 1031
BspANI GGCC 2 cut(s) 28, 499
BspCNI CTCAG 4 cut(s) 238, 571, 650, 844
BspLI GGNNCC 3 cut(s) 361, 432, 573
BspPI GGATC 1 cut(s) 175
BspQI GCTCTTC 1 cut(s) 368
BspT107I GGYRCC 1 cut(s) 359
BsrBI CCGCTC 1 cut(s) 224
BsrDI GCAATG 2 cut(s) 211, 236
BsrGI TGTACA 1 cut(s) 809
BsrI ACTGG 1 cut(s) 833
BssMI GATC 5 cut(s) 91, 143, 167, 292, 315
Bst2UI CCWGG 1 cut(s) 385
Bst4CI ACNGT 4 cut(s) 118, 448, 680, 1096
Bst6I CTCTTC 2 cut(s) 368, 612
BstAUI TGTACA 1 cut(s) 809
BstC8I GCNNGC 2 cut(s) 910, 1115
BstDEI CTNAG 6 cut(s) 225, 579, 637, 852, 977, 1163
BstF5I GGATG 2 cut(s) 135, 626
BstHHI GCGC 1 cut(s) 243
BstKTI GATC 5 cut(s) 94, 146, 170, 295, 318
BstMAI GTCTC 1 cut(s) 1092
BstMBI GATC 5 cut(s) 91, 143, 167, 292, 315
BstMCI CGRYCG 1 cut(s) 222
BstMWI GCNNNNNNNGC 2 cut(s) 561, 1010
BstNI CCWGG 1 cut(s) 385
BstNSI RCATGY 1 cut(s) 898
BstSCI CCNGG 1 cut(s) 383
BstSFI CTRYAG 1 cut(s) 30
BstV1I GCAGC 4 cut(s) 53, 500, 567, 722
BstV2I GAAGAC 2 cut(s) 475, 606
BstX2I RGATCY 2 cut(s) 143, 167
BstYI RGATCY 2 cut(s) 143, 167
BsuI GTATCC 1 cut(s) 1066
BsuRI GGCC 2 cut(s) 28, 499
BtgZI GCGATG 1 cut(s) 579
BtsCI GGATG 2 cut(s) 135, 626
BtsIMutI CAGTG 1 cut(s) 444
Cac8I GCNNGC 2 cut(s) 910, 1115
CaiI CAGNNNCTG 1 cut(s) 536
CfoI GCGC 1 cut(s) 243
Cfr13I GGNCC 1 cut(s) 26
CseI GACGC 1 cut(s) 851
Csp6I GTAC 4 cut(s) 482, 672, 810, 898
CspCI CAANNNNNGTGG 4 cut(s) 705, 740, 890, 925
CviAII CATG 6 cut(s) 131, 721, 895, 1018, 1106, 1180
CviQI GTAC 4 cut(s) 482, 672, 810, 898
DdeI CTNAG 6 cut(s) 225, 579, 637, 852, 977, 1163
DpnI GATC 5 cut(s) 93, 145, 169, 294, 317
DpnII GATC 5 cut(s) 91, 143, 167, 292, 315
Eam1104I CTCTTC 2 cut(s) 368, 612
EarI CTCTTC 2 cut(s) 368, 612
Ecl136II GAGCTC 1 cut(s) 877
Eco24I GRGCYC 2 cut(s) 433, 879
Eco53kI GAGCTC 1 cut(s) 877
Eco57I CTGAAG 3 cut(s) 513, 887, 1014
Eco88I CYCGRG 1 cut(s) 105
EcoICRI GAGCTC 1 cut(s) 877
EcoO109I RGGNCCY 1 cut(s) 26
EcoRII CCWGG 1 cut(s) 383
EcoT38I GRGCYC 2 cut(s) 433, 879
FaeI CATG 6 cut(s) 134, 724, 898, 1021, 1109, 1183
FalI AAGNNNNNCTT 4 cut(s) 133, 165, 906, 938
FaqI GGGAC 2 cut(s) 127, 547
FatI CATG 6 cut(s) 130, 720, 894, 1017, 1105, 1179
FauI CCCGC 1 cut(s) 104
Fnu4HI GCNGC 5 cut(s) 67, 489, 556, 711, 906
FokI GGATG 2 cut(s) 122, 613
FriOI GRGCYC 2 cut(s) 433, 879
Fsp4HI GCNGC 5 cut(s) 67, 489, 556, 711, 906
FspBI CTAG 3 cut(s) 356, 645, 735
GlaI GCGC 1 cut(s) 242
GluI GCNGC 5 cut(s) 67, 489, 556, 711, 906
GsuI CTGGAG 1 cut(s) 417
HaeIII GGCC 2 cut(s) 28, 499
HgaI GACGC 1 cut(s) 851
HhaI GCGC 1 cut(s) 243
Hin1II CATG 6 cut(s) 134, 724, 898, 1021, 1109, 1183
Hin6I GCGC 1 cut(s) 241
HinP1I GCGC 1 cut(s) 241
HincII GTYRAC 1 cut(s) 634
HindII GTYRAC 1 cut(s) 634
HinfI GANTC 7 cut(s) 185, 635, 641, 730, 738, 982, 1085
HphI GGTGA 3 cut(s) 540, 822, 1045
Hpy166II GTNNAC 3 cut(s) 482, 634, 652
Hpy188I TCNGA 4 cut(s) 193, 580, 640, 796
Hpy188III TCNNGA 4 cut(s) 296, 434, 594, 749
Hpy8I GTNNAC 3 cut(s) 482, 634, 652
Hpy99I CGWCG 1 cut(s) 608
HpyAV CCTTC 4 cut(s) 17, 703, 855, 1021
HpyCH4III ACNGT 4 cut(s) 118, 448, 680, 1096
HpyCH4IV ACGT 1 cut(s) 606
HpyCH4V TGCA 5 cut(s) 41, 444, 539, 710, 912
HpyF10VI GCNNNNNNNGC 2 cut(s) 561, 1010
HpyF3I CTNAG 6 cut(s) 225, 579, 637, 852, 977, 1163
HpySE526I ACGT 1 cut(s) 606
Hsp92II CATG 6 cut(s) 134, 724, 898, 1021, 1109, 1183
HspAI GCGC 1 cut(s) 241
Kzo9I GATC 5 cut(s) 91, 143, 167, 292, 315
LguI GCTCTTC 1 cut(s) 368
LmnI GCTCC 4 cut(s) 436, 577, 718, 1122
LpnPI CCDG 8 cut(s) 272, 344, 370, 397, 447, 516, 649, 846
Lsp1109I GCAGC 4 cut(s) 53, 500, 567, 722
MaeI CTAG 3 cut(s) 356, 645, 735
MaeII ACGT 1 cut(s) 606
MaeIII GTNAC 2 cut(s) 546, 659
MalI GATC 5 cut(s) 93, 145, 169, 294, 317
MbiI CCGCTC 1 cut(s) 224
MboI GATC 5 cut(s) 91, 143, 167, 292, 315
MfeI CAATTG 1 cut(s) 42
MflI RGATCY 2 cut(s) 143, 167
MhlI GDGCHC 2 cut(s) 433, 879
MluCI AATT 3 cut(s) 42, 334, 805
MlyI GAGTC 2 cut(s) 629, 1094
MmeI TCCRAC 1 cut(s) 705
MnlI CCTC 6 cut(s) 101, 114, 373, 516, 562, 586
MroXI GAANNNNTTC 1 cut(s) 472
MseI TTAA 3 cut(s) 78, 783, 1101
MslI CAYNNNNRTG 2 cut(s) 177, 1022
MspR9I CCNGG 1 cut(s) 385
MunI CAATTG 1 cut(s) 42
MvaI CCWGG 1 cut(s) 385
MwoI GCNNNNNNNGC 2 cut(s) 561, 1010
NdeII GATC 5 cut(s) 91, 143, 167, 292, 315
NlaIII CATG 6 cut(s) 134, 724, 898, 1021, 1109, 1183
NlaIV GGNNCC 3 cut(s) 361, 432, 573
NmeAIII GCCGAG 1 cut(s) 475
NmuCI GTSAC 1 cut(s) 546
NspI RCATGY 1 cut(s) 898
PaeR7I CTCGAG 1 cut(s) 105
PciI ACATGT 1 cut(s) 894
PciSI GCTCTTC 1 cut(s) 368
PdmI GAANNNNTTC 1 cut(s) 472
PfeI GAWTC 5 cut(s) 185, 641, 730, 738, 982
PkrI GCNGC 5 cut(s) 68, 490, 557, 712, 907
PleI GAGTC 2 cut(s) 629, 1093
PpsI GAGTC 2 cut(s) 629, 1093
PscI ACATGT 1 cut(s) 894
Psp124BI GAGCTC 1 cut(s) 879
Psp6I CCWGG 1 cut(s) 383
PspGI CCWGG 1 cut(s) 383
PspN4I GGNNCC 3 cut(s) 361, 432, 573
PspPI GGNCC 1 cut(s) 26
PspXI VCTCGAGB 1 cut(s) 105
PsrI GAACNNNNNNTAC 4 cut(s) 111, 143, 664, 696
PstNI CAGNNNCTG 1 cut(s) 536
PsuI RGATCY 2 cut(s) 143, 167
RsaI GTAC 4 cut(s) 483, 673, 811, 899
RsaNI GTAC 4 cut(s) 482, 672, 810, 898
RseI CAYNNNNRTG 2 cut(s) 177, 1022
SacI GAGCTC 1 cut(s) 879
SapI GCTCTTC 1 cut(s) 368
SaqAI TTAA 3 cut(s) 78, 783, 1101
SatI GCNGC 5 cut(s) 67, 489, 556, 711, 906
Sau3AI GATC 5 cut(s) 91, 143, 167, 292, 315
Sau96I GGNCC 1 cut(s) 26
SchI GAGTC 2 cut(s) 629, 1094
ScrFI CCNGG 1 cut(s) 385
SduI GDGCHC 2 cut(s) 433, 879
SfcI CTRYAG 1 cut(s) 30
Sfr274I CTCGAG 1 cut(s) 105
SlaI CTCGAG 1 cut(s) 105
SmiMI CAYNNNNRTG 2 cut(s) 177, 1022
SmlI CTYRAG 3 cut(s) 105, 507, 878
SmoI CTYRAG 3 cut(s) 105, 507, 878
Sse9I AATT 3 cut(s) 42, 334, 805
SsiI CCGC 4 cut(s) 111, 222, 906, 1031
SspI AATATT 1 cut(s) 157
SspMI CTAG 3 cut(s) 356, 645, 735
SstI GAGCTC 1 cut(s) 879
StyD4I CCNGG 1 cut(s) 383
TaaI ACNGT 4 cut(s) 118, 448, 680, 1096
TaiI ACGT 1 cut(s) 609
TaqI TCGA 4 cut(s) 90, 106, 318, 837
TasI AATT 3 cut(s) 42, 334, 805
TatI WGTACW 2 cut(s) 671, 809
TauI GCSGC 1 cut(s) 908
TfiI GAWTC 5 cut(s) 185, 641, 730, 738, 982
Tru1I TTAA 3 cut(s) 78, 783, 1101
Tru9I TTAA 3 cut(s) 78, 783, 1101
TscAI CASTG 1 cut(s) 451
TseFI GTSAC 1 cut(s) 546
TseI GCWGC 4 cut(s) 66, 488, 555, 710
Tsp45I GTSAC 1 cut(s) 546
TspDTI ATGAA 9 cut(s) 27, 119, 198, 340, 615, 813, 905, 1040, 1168
TspGWI ACGGA 2 cut(s) 229, 619
TspRI CASTG 1 cut(s) 451
XceI RCATGY 1 cut(s) 898
XhoI CTCGAG 1 cut(s) 105
XmnI GAANNNNTTC 1 cut(s) 472
XspI CTAG 3 cut(s) 356, 645, 735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.