RchiOBHm_Chr4g0397631

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0014277
Physical Location & Seq
Forward (+)
14033403 .. 14035968
2566 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36990

Sequence Viewer

Length: 1497 bp
ATGTTTGGATCAAAGAATAACATGTTTTCTGTAGACTCATTTAACTTGAAGGAAGGAGGAGGTGGGAGGGGGGAGAGAGTGATGTGGAATTCGAATAGCAGCAATAGTGGAAAAAGAAGACAATACAATGGGGGGCCAATAGCAGTAGGAGTATCAGGAGACAAAGCCAGCCAAAATGCTTTGAGATGGGCCATTGATAATCTTGTCACTCCTCCAAGCGACGGTGTTGTTGTCAAGCTTATCCATGTCGCTCAAGGATCACCATGTCCCATTGTCTCCCCTGTTGGGCATTGTGGGAATTTAGTGGGAAAGCCAAACTATTTACTTAAGCGTGGGCAAGCTTTTGATCCTATTGAGGAAATTTTACTTCCCTTTCGTTGCTATTGTACACGCAAACGAGTACCCTTTGAGATTGTTATACTCAAGGATCATGATGTAGCCAAGGCACTGATCGATCACATTTCCAGCCGTGGCATCGGGAATTTAATTCTTGGTGCTTCATCCAGGAATGGGTTTTCTAAGAGGTACGGCTACCATTACCAATCAATTTCATCTTTTCCATTTAGTGCTTTATCACATTATATCTGTTTCGTCGAATTAATGAACTTTTTTCTTTTTCTTTTTGGGATCTGCAGACTATTCAAGACATCAGATATTCCAAGTAGTGTGCAAAAGTTGGCACCGGATTTCTGCAATGTATTTACTATCAGCAAAGGAAAGATATGTGCAGTGCGTGCAGCCTGCCGCCGAGTGCCTGCCCTGCCGACCCTCCCGGAAGAATCTAATTACATAGCCGCAAGCAGCAAATCATATGACGAGGTCTCAGTGCAAGAGCCTGACATGGACACTGGCCACTCTTTTGTAGGCTCAGAAAGACGAAGTACTGATAGCATGTTGTATTCTTTCTACGAAAACTTTGGGCAAGCTGAATCGACCCAAATCTCTTCTATGTCCTTGAAGGTGGACATCACTGAATCAGCATCAGAATCAGATTTCGGGAGTTTCGATGGCCTTAACACTATGCATGAAATGCCATCAGCGGAGCAGCACAATGGCTCAGGAAATCCATTCTTGTCGCAGAAAGTACTGGAAGAGATGGAAGAAGAGATGAAAAGTCTTAGAATGGAGCTGAAGCAAACGATAGAGATGTACCATGCAGCCTATAAAGAAGCACTAACAGCAAAGCAGAAGGCTACAGAGCTCCAAGAGGGGAAAATGAAAGAAGAACTGAGGCTAGAGGAAGCACTACTCACCTTAGAAGCAACTACAGCAGCTTTGAAGAACGAAAAAGAAAAGTCTAAGGCAGCGATATTGGCAGTAGAAGCAGCTCAGAGGCTTGTTGAGGAAGAGGTGCAAAAGAGAGTGAATGCTGACAAGATGGAGGAGAACAAGGTTTTGGATACTTTTGGCCAGTTCTTTTTAGCTCTCAAGTACCAAAGCTTATTTCATATCGTAGTTGCTTTGATTTTATTCTATGTCTACTTCTCAATCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

498

Amino Acids

55.48

Weight (kDa)

6.3

Isoelectric Point (pI)

56.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014277)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20310
malus_domestica MD13G1232700.v1.1 MD13G1233300.v1.1 MD16G1238300.v1.1
prunus_persica Prupe.1G064200_v2.0.a1
pyrus_communis pycom13g20550
rosa_chinensis RchiOBHm_Chr4g0397631
rosa_laevigata RLG00000009422
rosa_multiflora Rmu_co8218642.1_g000001 Rmu_sc0002729.1_g000005
rosa_roxburghii Rroxscaffold_5G00342440
rosa_rugosa Rorug03G0342200
rosa_samantha Rh4AG079500 Rh4BG077500 Rh4CG085400 Rh4DG072500
rosa_wichuraiana Rw4G006510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 679
AccI GTMKAC 2 cut(s) 33, 1479
AciI CCGC 3 cut(s) 745, 795, 1040
AclWI GGATC 5 cut(s) 16, 265, 341, 435, 635
AcoI YGGCCR 2 cut(s) 850, 1408
AcsI RAATTY 4 cut(s) 88, 298, 360, 481
AcuI CTGAAG 1 cut(s) 1151
AfaI GTAC 7 cut(s) 388, 402, 527, 883, 1086, 1151, 1433
AfiI CCNNNNNNNGG 3 cut(s) 221, 285, 510
AflII CTTAAG 1 cut(s) 326
AflIII ACRYGT 1 cut(s) 21
AgsI TTSAA 4 cut(s) 49, 643, 958, 1279
AjnI CCWGG 1 cut(s) 503
AjuI GAANNNNNNNTTGG 4 cut(s) 1379, 1411, 1428, 1460
AluBI AGCT 9 cut(s) 238, 341, 926, 1129, 1201, 1274, 1328, 1424, 1440
AluI AGCT 9 cut(s) 238, 341, 926, 1129, 1201, 1274, 1328, 1424, 1440
Alw21I GWGCWC 1 cut(s) 1203
Alw26I GTCTC 3 cut(s) 153, 280, 826
AlwI GGATC 5 cut(s) 16, 265, 341, 435, 635
AoxI GGCC 5 cut(s) 134, 189, 850, 1009, 1408
ApeKI GCWGC 8 cut(s) 99, 737, 801, 1045, 1157, 1271, 1304, 1325
ApoI RAATTY 4 cut(s) 88, 298, 360, 481
AseI ATTAAT 1 cut(s) 599
AspS9I GGNCC 2 cut(s) 134, 189
AsuC2I CCSGG 1 cut(s) 773
AsuHPI GGTGA 2 cut(s) 252, 1243
AsuII TTCGAA 1 cut(s) 92
BalI TGGCCA 2 cut(s) 852, 1410
BanI GGYRCC 1 cut(s) 679
BanII GRGCYC 1 cut(s) 1203
BbsI GAAGAC 1 cut(s) 124
Bbv12I GWGCWC 1 cut(s) 1203
BbvI GCAGC 8 cut(s) 111, 749, 813, 1057, 1169, 1283, 1316, 1337
BccI CCATC 5 cut(s) 180, 1001, 1042, 1090, 1372
BceAI ACGGC 2 cut(s) 453, 544
BciT130I CCWGG 1 cut(s) 505
BciVI GTATCC 1 cut(s) 1393
BcnI CCSGG 1 cut(s) 773
BcoDI GTCTC 3 cut(s) 153, 280, 826
BfaI CTAG 1 cut(s) 1235
BfmI CTRYAG 4 cut(s) 30, 631, 1194, 1266
BfrI CTTAAG 1 cut(s) 326
BfuI GTATCC 1 cut(s) 1393
BmcAI AGTACT 2 cut(s) 883, 1086
Bme1390I CCNGG 2 cut(s) 505, 773
BmgT120I GGNCC 2 cut(s) 134, 189
BmiI GGNNCC 2 cut(s) 135, 681
BmrFI CCNGG 2 cut(s) 505, 773
BmsI GCATC 2 cut(s) 483, 989
BpiI GAAGAC 1 cut(s) 124
Bpu10I CCTNAGC 1 cut(s) 1057
Bpu14I TTCGAA 1 cut(s) 92
BpuEI CTTGAG 3 cut(s) 237, 407, 1412
BpuMI CCSGG 1 cut(s) 773
Bsa29I ATCGAT 1 cut(s) 453
BsaI GGTCTC 1 cut(s) 826
BsaJI CCNNGG 2 cut(s) 441, 469
BsaWI WCCGGW 1 cut(s) 682
Bsc4I CCNNNNNNNGG 3 cut(s) 221, 285, 510
Bse1I ACTGG 3 cut(s) 853, 1092, 1411
Bse3DI GCAATG 1 cut(s) 700
BseBI CCWGG 1 cut(s) 505
BseCI ATCGAT 1 cut(s) 453
BseDI CCNNGG 2 cut(s) 441, 469
BseGI GGATG 1 cut(s) 500
BseLI CCNNNNNNNGG 3 cut(s) 221, 285, 510
BseMI GCAATG 1 cut(s) 700
BseMII CTCAG 5 cut(s) 837, 882, 1071, 1220, 1343
BseNI ACTGG 3 cut(s) 853, 1092, 1411
BseRI GAGGAG 3 cut(s) 72, 201, 1397
BseXI GCAGC 8 cut(s) 111, 749, 813, 1057, 1169, 1283, 1316, 1337
BsgI GTGCAG 2 cut(s) 747, 756
BshFI GGCC 5 cut(s) 136, 191, 852, 1011, 1410
BshNI GGYRCC 1 cut(s) 679
BshVI ATCGAT 1 cut(s) 453
BsiHKAI GWGCWC 1 cut(s) 1203
BsiSI CCGG 2 cut(s) 683, 773
BslFI GGGAC 1 cut(s) 252
BslI CCNNNNNNNGG 3 cut(s) 221, 285, 510
BsmAI GTCTC 3 cut(s) 153, 280, 826
BsmFI GGGAC 1 cut(s) 252
BsmI GAATGC 1 cut(s) 1372
BsnI GGCC 5 cut(s) 136, 191, 852, 1011, 1410
Bso31I GGTCTC 1 cut(s) 826
Bsp119I TTCGAA 1 cut(s) 92
Bsp1286I GDGCHC 1 cut(s) 1203
Bsp1407I TGTACA 1 cut(s) 386
Bsp143I GATC 7 cut(s) 8, 257, 346, 427, 450, 454, 627
BspACI CCGC 3 cut(s) 745, 795, 1040
BspANI GGCC 5 cut(s) 136, 191, 852, 1011, 1410
BspCNI CTCAG 5 cut(s) 836, 881, 1070, 1221, 1342
BspDI ATCGAT 1 cut(s) 453
BspHI TCATGA 1 cut(s) 430
BspLI GGNNCC 2 cut(s) 135, 681
BspMAI CTGCAG 1 cut(s) 635
BspPI GGATC 5 cut(s) 16, 265, 341, 435, 635
BspT104I TTCGAA 1 cut(s) 92
BspT107I GGYRCC 1 cut(s) 679
BspTI CTTAAG 1 cut(s) 326
BspTNI GGTCTC 1 cut(s) 826
BsrDI GCAATG 1 cut(s) 700
BsrGI TGTACA 1 cut(s) 386
BsrI ACTGG 3 cut(s) 853, 1092, 1411
BssECI CCNNGG 2 cut(s) 441, 469
BssMI GATC 7 cut(s) 8, 257, 346, 427, 450, 454, 627
BssT1I CCWWGG 1 cut(s) 441
Bst2UI CCWGG 1 cut(s) 505
Bst4CI ACNGT 1 cut(s) 224
Bst6I CTCTTC 4 cut(s) 949, 1086, 1098, 1341
BstAFI CTTAAG 1 cut(s) 326
BstAPI GCANNNNNTGC 2 cut(s) 734, 1030
BstAUI TGTACA 1 cut(s) 386
BstBI TTCGAA 1 cut(s) 92
BstC8I GCNNGC 7 cut(s) 169, 339, 735, 742, 756, 799, 924
BstDEI CTNAG 9 cut(s) 519, 823, 868, 1057, 1118, 1229, 1255, 1299, 1329
BstDSI CCRYGG 1 cut(s) 469
BstF5I GGATG 1 cut(s) 500
BstKTI GATC 7 cut(s) 11, 260, 349, 430, 453, 457, 630
BstMAI GTCTC 3 cut(s) 153, 280, 826
BstMBI GATC 7 cut(s) 8, 257, 346, 427, 450, 454, 627
BstMWI GCNNNNNNNGC 7 cut(s) 734, 760, 1030, 1178, 1268, 1313, 1322
BstNI CCWGG 1 cut(s) 505
BstNSI RCATGY 2 cut(s) 25, 895
BstSCI CCNGG 2 cut(s) 503, 771
BstSFI CTRYAG 4 cut(s) 30, 631, 1194, 1266
BstV1I GCAGC 8 cut(s) 111, 749, 813, 1057, 1169, 1283, 1316, 1337
BstV2I GAAGAC 1 cut(s) 124
BstX2I RGATCY 1 cut(s) 627
BstYI RGATCY 1 cut(s) 627
Bsu15I ATCGAT 1 cut(s) 453
BsuI GTATCC 1 cut(s) 1393
BsuRI GGCC 5 cut(s) 136, 191, 852, 1011, 1410
BsuTUI ATCGAT 1 cut(s) 453
BtgI CCRYGG 1 cut(s) 469
BtsCI GGATG 1 cut(s) 500
BtsI GCAGTG 1 cut(s) 735
BtsIMutI CAGTG 5 cut(s) 446, 735, 831, 846, 969
Cac8I GCNNGC 7 cut(s) 169, 339, 735, 742, 756, 799, 924
CciI TCATGA 1 cut(s) 430
Cfr13I GGNCC 2 cut(s) 134, 189
ClaI ATCGAT 1 cut(s) 453
Csp6I GTAC 7 cut(s) 387, 401, 526, 882, 1085, 1150, 1432
CviAII CATG 8 cut(s) 22, 245, 264, 431, 841, 892, 1025, 1154
CviQI GTAC 7 cut(s) 387, 401, 526, 882, 1085, 1150, 1432
DdeI CTNAG 9 cut(s) 519, 823, 868, 1057, 1118, 1229, 1255, 1299, 1329
DpnI GATC 7 cut(s) 10, 259, 348, 429, 452, 456, 629
DpnII GATC 7 cut(s) 8, 257, 346, 427, 450, 454, 627
EaeI YGGCCR 2 cut(s) 850, 1408
Eam1104I CTCTTC 4 cut(s) 949, 1086, 1098, 1341
EarI CTCTTC 4 cut(s) 949, 1086, 1098, 1341
Ecl136II GAGCTC 1 cut(s) 1201
Eco130I CCWWGG 1 cut(s) 441
Eco24I GRGCYC 1 cut(s) 1203
Eco31I GGTCTC 1 cut(s) 826
Eco53kI GAGCTC 1 cut(s) 1201
Eco57I CTGAAG 1 cut(s) 1151
EcoICRI GAGCTC 1 cut(s) 1201
EcoRI GAATTC 1 cut(s) 88
EcoRII CCWGG 1 cut(s) 503
EcoT14I CCWWGG 1 cut(s) 441
EcoT22I ATGCAT 1 cut(s) 1026
EcoT38I GRGCYC 1 cut(s) 1203
ErhI CCWWGG 1 cut(s) 441
FaeI CATG 8 cut(s) 25, 248, 267, 434, 844, 895, 1028, 1157
FaqI GGGAC 1 cut(s) 252
FatI CATG 8 cut(s) 21, 244, 263, 430, 840, 891, 1024, 1153
FauNDI CATATG 1 cut(s) 811
FblI GTMKAC 2 cut(s) 33, 1479
FokI GGATG 1 cut(s) 487
FriOI GRGCYC 1 cut(s) 1203
FspBI CTAG 1 cut(s) 1235
HaeIII GGCC 5 cut(s) 136, 191, 852, 1011, 1410
HapII CCGG 2 cut(s) 683, 773
Hin1II CATG 8 cut(s) 25, 248, 267, 434, 844, 895, 1028, 1157
HindIII AAGCTT 3 cut(s) 236, 339, 1438
HinfI GANTC 5 cut(s) 35, 779, 929, 974, 986
HpaII CCGG 2 cut(s) 683, 773
HphI GGTGA 2 cut(s) 252, 1243
Hpy166II GTNNAC 4 cut(s) 34, 389, 964, 1480
Hpy188I TCNGA 5 cut(s) 652, 871, 985, 991, 1332
Hpy188III TCNNGA 6 cut(s) 156, 431, 478, 643, 997, 1059
Hpy8I GTNNAC 4 cut(s) 34, 389, 964, 1480
Hpy99I CGWCG 2 cut(s) 224, 596
HpyAV CCTTC 4 cut(s) 43, 47, 952, 1183
HpyCH4III ACNGT 1 cut(s) 224
HpyCH4V TGCA 9 cut(s) 633, 670, 693, 728, 737, 829, 1024, 1157, 1354
HpyF10VI GCNNNNNNNGC 7 cut(s) 734, 760, 1030, 1178, 1268, 1313, 1322
HpyF3I CTNAG 9 cut(s) 519, 823, 868, 1057, 1118, 1229, 1255, 1299, 1329
Hsp92II CATG 8 cut(s) 25, 248, 267, 434, 844, 895, 1028, 1157
Kzo9I GATC 7 cut(s) 8, 257, 346, 427, 450, 454, 627
LmnI GCTCC 3 cut(s) 1042, 1126, 1206
Lsp1109I GCAGC 8 cut(s) 111, 749, 813, 1057, 1169, 1283, 1316, 1337
LweI GCATC 2 cut(s) 483, 989
MaeI CTAG 1 cut(s) 1235
MaeIII GTNAC 1 cut(s) 205
MalI GATC 7 cut(s) 10, 259, 348, 429, 452, 456, 629
MboI GATC 7 cut(s) 8, 257, 346, 427, 450, 454, 627
MboII GAAGA 9 cut(s) 129, 788, 936, 1103, 1112, 1115, 1235, 1291, 1358
MflI RGATCY 1 cut(s) 627
MhlI GDGCHC 1 cut(s) 1203
MlsI TGGCCA 2 cut(s) 852, 1410
MluCI AATT 8 cut(s) 88, 298, 360, 481, 486, 546, 596, 784
MluNI TGGCCA 2 cut(s) 852, 1410
MlyI GAGTC 1 cut(s) 29
Mox20I TGGCCA 2 cut(s) 852, 1410
Mph1103I ATGCAT 1 cut(s) 1026
MscI TGGCCA 2 cut(s) 852, 1410
MseI TTAA 5 cut(s) 42, 327, 485, 599, 1014
Msp20I TGGCCA 2 cut(s) 852, 1410
MspA1I CMGCKG 1 cut(s) 1040
MspCI CTTAAG 1 cut(s) 326
MspI CCGG 2 cut(s) 683, 773
MspR9I CCNGG 2 cut(s) 505, 773
Mva1269I GAATGC 1 cut(s) 1372
MvaI CCWGG 1 cut(s) 505
MwoI GCNNNNNNNGC 7 cut(s) 734, 760, 1030, 1178, 1268, 1313, 1322
NciI CCSGG 1 cut(s) 773
NdeI CATATG 1 cut(s) 811
NdeII GATC 7 cut(s) 8, 257, 346, 427, 450, 454, 627
NlaIII CATG 8 cut(s) 25, 248, 267, 434, 844, 895, 1028, 1157
NlaIV GGNNCC 2 cut(s) 135, 681
NmeAIII GCCGAG 1 cut(s) 773
NmuCI GTSAC 1 cut(s) 205
NsiI ATGCAT 1 cut(s) 1026
NspI RCATGY 2 cut(s) 25, 895
NspV TTCGAA 1 cut(s) 92
PagI TCATGA 1 cut(s) 430
PciI ACATGT 1 cut(s) 21
PcsI WCGNNNNNNNCGW 1 cut(s) 1002
PctI GAATGC 1 cut(s) 1372
PfeI GAWTC 4 cut(s) 779, 929, 974, 986
PflFI GACNNNGTC 1 cut(s) 818
PfoI TCCNGGA 2 cut(s) 503, 771
PleI GAGTC 1 cut(s) 29
PpsI GAGTC 1 cut(s) 29
PscI ACATGT 1 cut(s) 21
PshBI ATTAAT 1 cut(s) 599
Psp124BI GAGCTC 1 cut(s) 1203
Psp6I CCWGG 1 cut(s) 503
PspGI CCWGG 1 cut(s) 503
PspN4I GGNNCC 2 cut(s) 135, 681
PspPI GGNCC 2 cut(s) 134, 189
PstI CTGCAG 1 cut(s) 635
PsuI RGATCY 1 cut(s) 627
PsyI GACNNNGTC 1 cut(s) 818
RsaI GTAC 7 cut(s) 388, 402, 527, 883, 1086, 1151, 1433
RsaNI GTAC 7 cut(s) 387, 401, 526, 882, 1085, 1150, 1432
SacI GAGCTC 1 cut(s) 1203
SaqAI TTAA 5 cut(s) 42, 327, 485, 599, 1014
Sau3AI GATC 7 cut(s) 8, 257, 346, 427, 450, 454, 627
Sau96I GGNCC 2 cut(s) 134, 189
ScaI AGTACT 2 cut(s) 883, 1086
SchI GAGTC 1 cut(s) 29
ScrFI CCNGG 2 cut(s) 505, 773
SduI GDGCHC 1 cut(s) 1203
SfaNI GCATC 2 cut(s) 483, 989
SfcI CTRYAG 4 cut(s) 30, 631, 1194, 1266
SfuI TTCGAA 1 cut(s) 92
SmlI CTYRAG 4 cut(s) 252, 326, 422, 1427
SmoI CTYRAG 4 cut(s) 252, 326, 422, 1427
Sse9I AATT 8 cut(s) 88, 298, 360, 481, 486, 546, 596, 784
SsiI CCGC 3 cut(s) 745, 795, 1040
SspMI CTAG 1 cut(s) 1235
SstI GAGCTC 1 cut(s) 1203
StyD4I CCNGG 2 cut(s) 503, 771
StyI CCWWGG 1 cut(s) 441
TaaI ACNGT 1 cut(s) 224
TaqI TCGA 5 cut(s) 92, 453, 594, 932, 1005
TasI AATT 8 cut(s) 88, 298, 360, 481, 486, 546, 596, 784
TatI WGTACW 3 cut(s) 386, 881, 1084
TauI GCSGC 2 cut(s) 747, 797
TfiI GAWTC 4 cut(s) 779, 929, 974, 986
Tru1I TTAA 5 cut(s) 42, 327, 485, 599, 1014
Tru9I TTAA 5 cut(s) 42, 327, 485, 599, 1014
TscAI CASTG 5 cut(s) 453, 735, 831, 853, 976
TseFI GTSAC 1 cut(s) 205
TseI GCWGC 8 cut(s) 99, 737, 801, 1045, 1157, 1271, 1304, 1325
Tsp45I GTSAC 1 cut(s) 205
TspDTI ATGAA 7 cut(s) 489, 540, 617, 1041, 1124, 1232, 1436
TspRI CASTG 5 cut(s) 453, 735, 831, 853, 976
Tth111I GACNNNGTC 1 cut(s) 818
Vha464I CTTAAG 1 cut(s) 326
VspI ATTAAT 1 cut(s) 599
XapI RAATTY 4 cut(s) 88, 298, 360, 481
XceI RCATGY 2 cut(s) 25, 895
XmiI GTMKAC 2 cut(s) 33, 1479
XspI CTAG 1 cut(s) 1235
ZrmI AGTACT 2 cut(s) 883, 1086
Zsp2I ATGCAT 1 cut(s) 1026
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.