Rw4G006510

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014277
Physical Location & Seq
Forward (+)
13579441 .. 13582434
2994 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G006510.1

Sequence Viewer

Length: 1305 bp
ATGTTTTCTGTAGACTCATTTAACTTGAAGGAAGGAGGAGGAGGGAGGGGGGAGAGAGTGTTGTGGAATTCGAATAGCAGCAATAGTGGAAAAAGAAGACAATACAATGGGGGGCCAATAGCAGTAGGAGTATCAGGAGACAAAGCCAGCCAAAATGCTTTGAGATGGGCCATTGATAATCTTGTCACTCCTCCAAGCGACGATGTTGTTGTCAAGCTTATCCATGTCGCTCAAGGATCACCATGTCCCATTGTCTCCCCTGGGAACAGTGGAATGATTAATGAAGGGCAAGCTTTTGATCCTATTGAGGAAATTTTACTTCCCTTTCGTTGCTATTGTACACGCAAACGAGTACCATATGAGATTGTTATACTCAAGGATCATGATGTAGCCAAGGCACTGATCGATTACATTTCCCGCCGTGGCATCGGGAATTTAATTCTTGGTGCTTCATCCAGGAATGGGTTTTCTAAGAGACTATTCAAGACATCAGATATTCCAAGTAGTGTGCAAAAGTTGGCACCGGATTTCTGCAATGTATTTACTATCAGCAAAGGAAAGATATGTGCAGTGCGTGCAGCCTGCCGCCGAGTGCCTGCCCTGCCGACCCTCCCGGAAGAATCTAATTACTTAGGCAGAGCCGCAAGCAGCAAATCATATGACGAGGTCTCAGTGCAAGAGCCTGACATGGACACTGGCCTCTCTTTTGTAGGCTCAGAAAGACAAAGTACTGATAGCATGTTGTATTCTTTCTACGAAAACTTTGGGCAAGCTGAATTGACCCAAATCTCTTCTATGTCCTTGAACGTGGACATCACTGAATCAGCATCAGAATCAGATTTCGGGAGTTTCGATGGCCTTAACACTTTGCATGAAATGACATCAGCGGAGCAGCACAATGGCTCAGGAAATCCATTCTTGTCGCAGAAAGTACTGGAAGAGATGGAAGAAGAGATGAAAAGTCTTAGAATGGAGCTGAAGCAAACGATAGAGATGTACCATGCAGCCTATAAAGAAGCACTAACAGCAAAGCAGAAGGCTACAGAGCTCCAAGAGGGGAAAATGAAAGAAGAACAGAGACTAGAGGAAGCACTACTCACCTTAGAAGCAACTACAGCAGCTTTGAAGAACGAAAAAGAAAAGTCTAAGGCAGCGATATTGGCAGCAGAAGCAGCTCAGAGGCTTGTTGAGAAAGAGGTGCAAAAGAGAGTGAATGCTGACAAGATGGAGGAGAACAAGGTTTTGGATACTTTTGGCCAGTTCTTTTTAGCTCTCAAGTACCAAAGCTTATTTCATATCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

434

Amino Acids

47.95

Weight (kDa)

5.53

Isoelectric Point (pI)

53.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 39 - 161 7.5e-06 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014277)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20310
malus_domestica MD13G1232700.v1.1 MD13G1233300.v1.1 MD16G1238300.v1.1
prunus_persica Prupe.1G064200_v2.0.a1
pyrus_communis pycom13g20550
rosa_chinensis RchiOBHm_Chr4g0397631
rosa_laevigata RLG00000009422
rosa_multiflora Rmu_co8218642.1_g000001 Rmu_sc0002729.1_g000005
rosa_roxburghii Rroxscaffold_5G00342440
rosa_rugosa Rorug03G0342200
rosa_samantha Rh4AG079500 Rh4BG077500 Rh4CG085400 Rh4DG072500
rosa_wichuraiana Rw4G006510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 520
AccI GTMKAC 1 cut(s) 12
AciI CCGC 4 cut(s) 418, 586, 642, 887
AclWI GGATC 3 cut(s) 244, 293, 387
AcoI YGGCCR 1 cut(s) 1255
AcsI RAATTY 3 cut(s) 67, 312, 433
AcuI CTGAAG 1 cut(s) 998
AfaI GTAC 6 cut(s) 340, 354, 730, 933, 998, 1280
AfiI CCNNNNNNNGG 1 cut(s) 462
AgsI TTSAA 4 cut(s) 28, 484, 805, 1126
AjnI CCWGG 2 cut(s) 259, 455
AjuI GAANNNNNNNTTGG 3 cut(s) 1226, 1258, 1275
AluBI AGCT 9 cut(s) 217, 293, 773, 976, 1048, 1121, 1175, 1271, 1287
AluI AGCT 9 cut(s) 217, 293, 773, 976, 1048, 1121, 1175, 1271, 1287
Alw21I GWGCWC 1 cut(s) 1050
Alw26I GTCTC 5 cut(s) 132, 259, 469, 673, 1072
AlwI GGATC 3 cut(s) 244, 293, 387
AoxI GGCC 5 cut(s) 113, 168, 697, 856, 1255
ApeKI GCWGC 9 cut(s) 78, 578, 648, 892, 1004, 1118, 1151, 1163, 1172
ApoI RAATTY 3 cut(s) 67, 312, 433
AseI ATTAAT 1 cut(s) 279
AspS9I GGNCC 2 cut(s) 113, 168
AsuC2I CCSGG 1 cut(s) 614
AsuHPI GGTGA 2 cut(s) 231, 1090
AsuII TTCGAA 1 cut(s) 71
BalI TGGCCA 1 cut(s) 1257
BanI GGYRCC 1 cut(s) 520
BanII GRGCYC 1 cut(s) 1050
BbsI GAAGAC 1 cut(s) 103
Bbv12I GWGCWC 1 cut(s) 1050
BbvI GCAGC 9 cut(s) 90, 590, 660, 904, 1016, 1130, 1163, 1175, 1184
BccI CCATC 4 cut(s) 159, 848, 937, 1219
BceAI ACGGC 1 cut(s) 405
BciT130I CCWGG 2 cut(s) 261, 457
BciVI GTATCC 1 cut(s) 1240
BcnI CCSGG 1 cut(s) 614
BcoDI GTCTC 5 cut(s) 132, 259, 469, 673, 1072
BfaI CTAG 1 cut(s) 1082
BfmI CTRYAG 3 cut(s) 9, 1041, 1113
BfuI GTATCC 1 cut(s) 1240
BmcAI AGTACT 2 cut(s) 730, 933
Bme1390I CCNGG 3 cut(s) 261, 457, 614
BmgT120I GGNCC 2 cut(s) 113, 168
BmiI GGNNCC 2 cut(s) 114, 522
BmrFI CCNGG 3 cut(s) 261, 457, 614
BmsI GCATC 2 cut(s) 435, 836
BpiI GAAGAC 1 cut(s) 103
Bpu10I CCTNAGC 1 cut(s) 904
Bpu14I TTCGAA 1 cut(s) 71
BpuEI CTTGAG 3 cut(s) 216, 359, 1259
BpuMI CCSGG 1 cut(s) 614
Bsa29I ATCGAT 1 cut(s) 405
BsaI GGTCTC 1 cut(s) 673
BsaJI CCNNGG 4 cut(s) 259, 260, 393, 421
BsaWI WCCGGW 1 cut(s) 523
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 1 cut(s) 462
Bse1I ACTGG 3 cut(s) 700, 939, 1258
Bse3DI GCAATG 1 cut(s) 541
BseBI CCWGG 2 cut(s) 261, 457
BseCI ATCGAT 1 cut(s) 405
BseDI CCNNGG 4 cut(s) 259, 260, 393, 421
BseGI GGATG 1 cut(s) 452
BseLI CCNNNNNNNGG 1 cut(s) 462
BseMI GCAATG 1 cut(s) 541
BseMII CTCAG 4 cut(s) 684, 729, 918, 1190
BseNI ACTGG 3 cut(s) 700, 939, 1258
BseRI GAGGAG 4 cut(s) 51, 54, 180, 1244
BseXI GCAGC 9 cut(s) 90, 590, 660, 904, 1016, 1130, 1163, 1175, 1184
BsgI GTGCAG 2 cut(s) 588, 597
BshFI GGCC 5 cut(s) 115, 170, 699, 858, 1257
BshNI GGYRCC 1 cut(s) 520
BshVI ATCGAT 1 cut(s) 405
BsiHKAI GWGCWC 1 cut(s) 1050
BsiSI CCGG 2 cut(s) 524, 614
BslFI GGGAC 1 cut(s) 231
BslI CCNNNNNNNGG 1 cut(s) 462
BsmAI GTCTC 5 cut(s) 132, 259, 469, 673, 1072
BsmFI GGGAC 1 cut(s) 231
BsmI GAATGC 1 cut(s) 1219
BsnI GGCC 5 cut(s) 115, 170, 699, 858, 1257
Bso31I GGTCTC 1 cut(s) 673
Bsp119I TTCGAA 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 1050
Bsp1407I TGTACA 1 cut(s) 338
Bsp143I GATC 4 cut(s) 236, 298, 379, 402
BspACI CCGC 4 cut(s) 418, 586, 642, 887
BspANI GGCC 5 cut(s) 115, 170, 699, 858, 1257
BspCNI CTCAG 4 cut(s) 683, 728, 917, 1189
BspDI ATCGAT 1 cut(s) 405
BspHI TCATGA 1 cut(s) 382
BspLI GGNNCC 2 cut(s) 114, 522
BspPI GGATC 3 cut(s) 244, 293, 387
BspT104I TTCGAA 1 cut(s) 71
BspT107I GGYRCC 1 cut(s) 520
BspTNI GGTCTC 1 cut(s) 673
BsrDI GCAATG 1 cut(s) 541
BsrGI TGTACA 1 cut(s) 338
BsrI ACTGG 3 cut(s) 700, 939, 1258
BssECI CCNNGG 4 cut(s) 259, 260, 393, 421
BssMI GATC 4 cut(s) 236, 298, 379, 402
BssT1I CCWWGG 1 cut(s) 393
Bst2UI CCWGG 2 cut(s) 261, 457
Bst4CI ACNGT 1 cut(s) 269
Bst6I CTCTTC 3 cut(s) 796, 933, 945
BstAPI GCANNNNNTGC 1 cut(s) 575
BstAUI TGTACA 1 cut(s) 338
BstBI TTCGAA 1 cut(s) 71
BstC8I GCNNGC 7 cut(s) 148, 291, 576, 583, 597, 646, 771
BstDEI CTNAG 9 cut(s) 471, 631, 670, 715, 904, 965, 1102, 1146, 1176
BstDSI CCRYGG 1 cut(s) 421
BstF5I GGATG 1 cut(s) 452
BstKTI GATC 4 cut(s) 239, 301, 382, 405
BstMAI GTCTC 5 cut(s) 132, 259, 469, 673, 1072
BstMBI GATC 4 cut(s) 236, 298, 379, 402
BstMWI GCNNNNNNNGC 7 cut(s) 575, 601, 1025, 1115, 1160, 1169, 1172
BstNI CCWGG 2 cut(s) 261, 457
BstNSI RCATGY 1 cut(s) 742
BstSCI CCNGG 3 cut(s) 259, 455, 612
BstSFI CTRYAG 3 cut(s) 9, 1041, 1113
BstV1I GCAGC 9 cut(s) 90, 590, 660, 904, 1016, 1130, 1163, 1175, 1184
BstV2I GAAGAC 1 cut(s) 103
Bsu15I ATCGAT 1 cut(s) 405
BsuI GTATCC 1 cut(s) 1240
BsuRI GGCC 5 cut(s) 115, 170, 699, 858, 1257
BsuTUI ATCGAT 1 cut(s) 405
BtgI CCRYGG 1 cut(s) 421
BtsCI GGATG 1 cut(s) 452
BtsI GCAGTG 1 cut(s) 576
BtsIMutI CAGTG 6 cut(s) 274, 398, 576, 678, 693, 816
Cac8I GCNNGC 7 cut(s) 148, 291, 576, 583, 597, 646, 771
CciI TCATGA 1 cut(s) 382
Cfr13I GGNCC 2 cut(s) 113, 168
ClaI ATCGAT 1 cut(s) 405
Csp6I GTAC 6 cut(s) 339, 353, 729, 932, 997, 1279
CviAII CATG 7 cut(s) 224, 243, 383, 688, 739, 872, 1001
CviQI GTAC 6 cut(s) 339, 353, 729, 932, 997, 1279
DdeI CTNAG 9 cut(s) 471, 631, 670, 715, 904, 965, 1102, 1146, 1176
DpnI GATC 4 cut(s) 238, 300, 381, 404
DpnII GATC 4 cut(s) 236, 298, 379, 402
EaeI YGGCCR 1 cut(s) 1255
Eam1104I CTCTTC 3 cut(s) 796, 933, 945
EarI CTCTTC 3 cut(s) 796, 933, 945
Ecl136II GAGCTC 1 cut(s) 1048
Eco130I CCWWGG 1 cut(s) 393
Eco24I GRGCYC 1 cut(s) 1050
Eco31I GGTCTC 1 cut(s) 673
Eco53kI GAGCTC 1 cut(s) 1048
Eco57I CTGAAG 1 cut(s) 998
EcoICRI GAGCTC 1 cut(s) 1048
EcoRI GAATTC 1 cut(s) 67
EcoRII CCWGG 2 cut(s) 259, 455
EcoT14I CCWWGG 1 cut(s) 393
EcoT38I GRGCYC 1 cut(s) 1050
ErhI CCWWGG 1 cut(s) 393
FaeI CATG 7 cut(s) 227, 246, 386, 691, 742, 875, 1004
FaqI GGGAC 1 cut(s) 231
FatI CATG 7 cut(s) 223, 242, 382, 687, 738, 871, 1000
FauI CCCGC 1 cut(s) 425
FauNDI CATATG 3 cut(s) 358, 658, 1301
FblI GTMKAC 1 cut(s) 12
FokI GGATG 1 cut(s) 439
FriOI GRGCYC 1 cut(s) 1050
FspBI CTAG 1 cut(s) 1082
HaeIII GGCC 5 cut(s) 115, 170, 699, 858, 1257
HapII CCGG 2 cut(s) 524, 614
Hin1II CATG 7 cut(s) 227, 246, 386, 691, 742, 875, 1004
HindIII AAGCTT 3 cut(s) 215, 291, 1285
HinfI GANTC 4 cut(s) 14, 620, 821, 833
HpaII CCGG 2 cut(s) 524, 614
HphI GGTGA 2 cut(s) 231, 1090
Hpy166II GTNNAC 3 cut(s) 13, 341, 811
Hpy188I TCNGA 5 cut(s) 493, 718, 832, 838, 1179
Hpy188III TCNNGA 6 cut(s) 135, 383, 430, 484, 844, 906
Hpy8I GTNNAC 3 cut(s) 13, 341, 811
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 4 cut(s) 22, 26, 278, 1030
HpyCH4III ACNGT 1 cut(s) 269
HpyCH4IV ACGT 1 cut(s) 807
HpyCH4V TGCA 8 cut(s) 511, 534, 569, 578, 676, 871, 1004, 1201
HpyF10VI GCNNNNNNNGC 7 cut(s) 575, 601, 1025, 1115, 1160, 1169, 1172
HpyF3I CTNAG 9 cut(s) 471, 631, 670, 715, 904, 965, 1102, 1146, 1176
HpySE526I ACGT 1 cut(s) 807
Hsp92II CATG 7 cut(s) 227, 246, 386, 691, 742, 875, 1004
Kzo9I GATC 4 cut(s) 236, 298, 379, 402
LmnI GCTCC 3 cut(s) 889, 973, 1053
Lsp1109I GCAGC 9 cut(s) 90, 590, 660, 904, 1016, 1130, 1163, 1175, 1184
LweI GCATC 2 cut(s) 435, 836
MaeI CTAG 1 cut(s) 1082
MaeII ACGT 1 cut(s) 807
MaeIII GTNAC 1 cut(s) 184
MalI GATC 4 cut(s) 238, 300, 381, 404
MboI GATC 4 cut(s) 236, 298, 379, 402
MboII GAAGA 8 cut(s) 108, 629, 783, 950, 959, 962, 1082, 1138
MhlI GDGCHC 1 cut(s) 1050
MlsI TGGCCA 1 cut(s) 1257
MluCI AATT 6 cut(s) 67, 312, 433, 438, 625, 776
MluNI TGGCCA 1 cut(s) 1257
MlyI GAGTC 1 cut(s) 8
Mox20I TGGCCA 1 cut(s) 1257
MscI TGGCCA 1 cut(s) 1257
MseI TTAA 4 cut(s) 21, 279, 437, 861
Msp20I TGGCCA 1 cut(s) 1257
MspA1I CMGCKG 1 cut(s) 887
MspI CCGG 2 cut(s) 524, 614
MspR9I CCNGG 3 cut(s) 261, 457, 614
Mva1269I GAATGC 1 cut(s) 1219
MvaI CCWGG 2 cut(s) 261, 457
MwoI GCNNNNNNNGC 7 cut(s) 575, 601, 1025, 1115, 1160, 1169, 1172
NciI CCSGG 1 cut(s) 614
NdeI CATATG 3 cut(s) 358, 658, 1301
NdeII GATC 4 cut(s) 236, 298, 379, 402
NlaIII CATG 7 cut(s) 227, 246, 386, 691, 742, 875, 1004
NlaIV GGNNCC 2 cut(s) 114, 522
NmeAIII GCCGAG 1 cut(s) 614
NmuCI GTSAC 1 cut(s) 184
NspI RCATGY 1 cut(s) 742
NspV TTCGAA 1 cut(s) 71
PagI TCATGA 1 cut(s) 382
PasI CCCWGGG 1 cut(s) 260
PcsI WCGNNNNNNNCGW 1 cut(s) 849
PctI GAATGC 1 cut(s) 1219
PfeI GAWTC 3 cut(s) 620, 821, 833
PflFI GACNNNGTC 1 cut(s) 665
PfoI TCCNGGA 2 cut(s) 455, 612
PleI GAGTC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 8
PshBI ATTAAT 1 cut(s) 279
Psp124BI GAGCTC 1 cut(s) 1050
Psp6I CCWGG 2 cut(s) 259, 455
PspGI CCWGG 2 cut(s) 259, 455
PspN4I GGNNCC 2 cut(s) 114, 522
PspPI GGNCC 2 cut(s) 113, 168
PsyI GACNNNGTC 1 cut(s) 665
RsaI GTAC 6 cut(s) 340, 354, 730, 933, 998, 1280
RsaNI GTAC 6 cut(s) 339, 353, 729, 932, 997, 1279
SacI GAGCTC 1 cut(s) 1050
SaqAI TTAA 4 cut(s) 21, 279, 437, 861
Sau3AI GATC 4 cut(s) 236, 298, 379, 402
Sau96I GGNCC 2 cut(s) 113, 168
ScaI AGTACT 2 cut(s) 730, 933
SchI GAGTC 1 cut(s) 8
ScrFI CCNGG 3 cut(s) 261, 457, 614
SduI GDGCHC 1 cut(s) 1050
SfaNI GCATC 2 cut(s) 435, 836
SfcI CTRYAG 3 cut(s) 9, 1041, 1113
SfuI TTCGAA 1 cut(s) 71
SmlI CTYRAG 3 cut(s) 231, 374, 1274
SmoI CTYRAG 3 cut(s) 231, 374, 1274
Sse9I AATT 6 cut(s) 67, 312, 433, 438, 625, 776
SsiI CCGC 4 cut(s) 418, 586, 642, 887
SspMI CTAG 1 cut(s) 1082
SstI GAGCTC 1 cut(s) 1050
StyD4I CCNGG 3 cut(s) 259, 455, 612
StyI CCWWGG 1 cut(s) 393
TaaI ACNGT 1 cut(s) 269
TaiI ACGT 1 cut(s) 810
TaqI TCGA 3 cut(s) 71, 405, 852
TasI AATT 6 cut(s) 67, 312, 433, 438, 625, 776
TatI WGTACW 3 cut(s) 338, 728, 931
TauI GCSGC 2 cut(s) 588, 644
TfiI GAWTC 3 cut(s) 620, 821, 833
Tru1I TTAA 4 cut(s) 21, 279, 437, 861
Tru9I TTAA 4 cut(s) 21, 279, 437, 861
TscAI CASTG 6 cut(s) 274, 405, 576, 678, 700, 823
TseFI GTSAC 1 cut(s) 184
TseI GCWGC 9 cut(s) 78, 578, 648, 892, 1004, 1118, 1151, 1163, 1172
Tsp45I GTSAC 1 cut(s) 184
TspDTI ATGAA 6 cut(s) 297, 441, 888, 971, 1079, 1283
TspRI CASTG 6 cut(s) 274, 405, 576, 678, 700, 823
Tth111I GACNNNGTC 1 cut(s) 665
VspI ATTAAT 1 cut(s) 279
XapI RAATTY 3 cut(s) 67, 312, 433
XceI RCATGY 1 cut(s) 742
XmiI GTMKAC 1 cut(s) 12
XspI CTAG 1 cut(s) 1082
ZrmI AGTACT 2 cut(s) 730, 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.