pycom13g20550

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0014277
Physical Location & Seq
Forward (+)
16665789 .. 16668616
2828 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g20550.1

Sequence Viewer

Length: 912 bp
ATGTTCCCATATTCATGTTTGAGCAAGAACTTGCCATATTCATGTCTGAGCAAGAAGTTGTTCAATCTGAGTGTTGAGAAAGAGGAAGGGCGAAAGACAATAATGTGGTATAACAACGTTGATAATGGAAATAATAATCAAGGCGCGGGGATAATAGCAGTGGCAATCGATGATGGCGTCTCCAGCCAAAATGCTTTGAAATGGGCAATTGATCATCTCGTCTCTCGGTCTACCAGCCATGCTCCTATCAAGCTTGTCCATGTTATTCACCCATCTAATTCCAATAATACACTTCATGCAACTGGGCAAGCTCACGATGCTCCAAATACAGATTTCTTACTTCCATTTCGTTGCTACTGTACGCGCAAACGAGTAGGGCGTCTATTCAAGGCATCTGAATCTATTCCAGGCAGAGTCCTGAAGTTGGCACCACATTTCTGCTCAGTATACACCATCAAACAAGGAAGGGATGACATGGAAGAGGAGATGAAAAGGCTTAGAGTGGAGCTGAAGCAAACAATGGACATGTACCACGCAGCCTGCAAAGAAGCAGTGGCAGCAAAACAGAAGACAATTGAGCTACAAGAGTGGAAATTGAAAGAAGGGAAAAAACTAGAAGAGGCACGATATGCCTTAGAAACAACTAGGGAAGCTATGGAGAAAGAAAAAGCACATTCTAAAGCAGCAATTTTGACAGCTGATCATCGTCACGAAGTAGCTCAGAGGTTTGCTAAGAAAGAGGTGACAATCAGTGCAGATCAAATATTCAAAGCTTTAAGGGAGGCTGACGATGAAGAGAAGAAAAGAGTTTTGGATGCTTTAGGCCAGTCCCAAATAGTTTTCAAATACCAAAGCTTAGTTCATATCTTAGTTGTTTTATTGTTATTTTCTTTTTACTGTTCACTGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

304

Amino Acids

34.5

Weight (kDa)

9.0

Isoelectric Point (pI)

38.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014277)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20310
malus_domestica MD13G1232700.v1.1 MD13G1233300.v1.1 MD16G1238300.v1.1
prunus_persica Prupe.1G064200_v2.0.a1
pyrus_communis pycom13g20550
rosa_chinensis RchiOBHm_Chr4g0397631
rosa_laevigata RLG00000009422
rosa_multiflora Rmu_co8218642.1_g000001 Rmu_sc0002729.1_g000005
rosa_roxburghii Rroxscaffold_5G00342440
rosa_rugosa Rorug03G0342200
rosa_samantha Rh4AG079500 Rh4BG077500 Rh4CG085400 Rh4DG072500
rosa_wichuraiana Rw4G006510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 427
AccI GTMKAC 2 cut(s) 230, 447
AccII CGCG 2 cut(s) 146, 364
AciI CCGC 1 cut(s) 146
AclI AACGTT 1 cut(s) 117
AcuI CTGAAG 2 cut(s) 440, 530
AcyI GRCGYC 2 cut(s) 177, 379
AfaI GTAC 2 cut(s) 361, 530
AfiI CCNNNNNNNGG 1 cut(s) 424
AflIII ACRYGT 1 cut(s) 525
AgsI TTSAA 6 cut(s) 64, 199, 388, 598, 769, 844
AjnI CCWGG 1 cut(s) 406
AjuI GAANNNNNNNTTGG 4 cut(s) 794, 826, 843, 875
AluBI AGCT 9 cut(s) 253, 311, 508, 580, 653, 698, 719, 773, 855
AluI AGCT 9 cut(s) 253, 311, 508, 580, 653, 698, 719, 773, 855
Alw26I GTCTC 2 cut(s) 184, 226
AoxI GGCC 1 cut(s) 823
ApeKI GCWGC 3 cut(s) 536, 557, 683
Asp700I GAANNNNTTC 2 cut(s) 59, 402
AspLEI GCGC 2 cut(s) 146, 366
AsuHPI GGTGA 2 cut(s) 260, 754
BanI GGYRCC 1 cut(s) 427
BbsI GAAGAC 1 cut(s) 575
BbvI GCAGC 3 cut(s) 548, 569, 695
BccI CCATC 3 cut(s) 167, 280, 461
BciT130I CCWGG 1 cut(s) 408
BclI TGATCA 2 cut(s) 211, 700
BcoDI GTCTC 2 cut(s) 184, 226
BfaI CTAG 2 cut(s) 614, 645
BisI GCNGC 3 cut(s) 537, 558, 684
BlsI GCNGC 3 cut(s) 538, 559, 685
Bme1390I CCNGG 1 cut(s) 408
BmiI GGNNCC 1 cut(s) 429
BmrFI CCNGG 1 cut(s) 408
BmrI ACTGGG 1 cut(s) 312
BmsI GCATC 3 cut(s) 307, 401, 805
BmuI ACTGGG 1 cut(s) 312
BpiI GAAGAC 1 cut(s) 575
BpmI CTGGAG 1 cut(s) 166
Bsa29I ATCGAT 1 cut(s) 168
BsaHI GRCGYC 2 cut(s) 177, 379
Bsc4I CCNNNNNNNGG 1 cut(s) 424
Bse1I ACTGG 2 cut(s) 307, 826
BseBI CCWGG 1 cut(s) 408
BseCI ATCGAT 1 cut(s) 168
BseGI GGATG 2 cut(s) 475, 820
BseLI CCNNNNNNNGG 1 cut(s) 424
BseMII CTCAG 4 cut(s) 38, 59, 456, 734
BseNI ACTGG 2 cut(s) 307, 826
BseRI GAGGAG 1 cut(s) 497
BseXI GCAGC 3 cut(s) 548, 569, 695
BsgI GTGCAG 1 cut(s) 774
Bsh1236I CGCG 2 cut(s) 146, 364
BshFI GGCC 1 cut(s) 825
BshNI GGYRCC 1 cut(s) 427
BshVI ATCGAT 1 cut(s) 168
BslFI GGGAC 1 cut(s) 814
BslI CCNNNNNNNGG 1 cut(s) 424
BsmAI GTCTC 2 cut(s) 184, 226
BsmBI CGTCTC 2 cut(s) 184, 226
BsmFI GGGAC 1 cut(s) 814
BsnI GGCC 1 cut(s) 825
Bsp143I GATC 3 cut(s) 211, 700, 757
BspACI CCGC 1 cut(s) 146
BspANI GGCC 1 cut(s) 825
BspCNI CTCAG 4 cut(s) 39, 60, 455, 733
BspDI ATCGAT 1 cut(s) 168
BspFNI CGCG 2 cut(s) 146, 364
BspLI GGNNCC 1 cut(s) 429
BspT107I GGYRCC 1 cut(s) 427
BsrI ACTGG 2 cut(s) 307, 826
BssMI GATC 3 cut(s) 211, 700, 757
BssNAI GTATAC 1 cut(s) 448
BssNI GRCGYC 2 cut(s) 177, 379
Bst1107I GTATAC 1 cut(s) 448
Bst2UI CCWGG 1 cut(s) 408
Bst4CI ACNGT 3 cut(s) 359, 899, 906
Bst6I CTCTTC 3 cut(s) 474, 612, 789
BstACI GRCGYC 2 cut(s) 177, 379
BstAPI GCANNNNNTGC 1 cut(s) 629
BstC8I GCNNGC 2 cut(s) 309, 541
BstDEI CTNAG 9 cut(s) 47, 68, 442, 497, 634, 720, 732, 856, 868
BstF5I GGATG 2 cut(s) 475, 820
BstFNI CGCG 2 cut(s) 146, 364
BstHHI GCGC 2 cut(s) 146, 366
BstKTI GATC 3 cut(s) 214, 703, 760
BstMAI GTCTC 2 cut(s) 184, 226
BstMBI GATC 3 cut(s) 211, 700, 757
BstMWI GCNNNNNNNGC 4 cut(s) 183, 317, 557, 629
BstNI CCWGG 1 cut(s) 408
BstNSI RCATGY 1 cut(s) 529
BstSCI CCNGG 1 cut(s) 406
BstUI CGCG 2 cut(s) 146, 364
BstV1I GCAGC 3 cut(s) 548, 569, 695
BstV2I GAAGAC 1 cut(s) 575
BstZ17I GTATAC 1 cut(s) 448
Bsu15I ATCGAT 1 cut(s) 168
BsuRI GGCC 1 cut(s) 825
BsuTUI ATCGAT 1 cut(s) 168
BtsCI GGATG 2 cut(s) 475, 820
BtsI GCAGTG 2 cut(s) 165, 558
BtsIMutI CAGTG 4 cut(s) 165, 558, 757, 902
Cac8I GCNNGC 2 cut(s) 309, 541
CfoI GCGC 2 cut(s) 146, 366
ClaI ATCGAT 1 cut(s) 168
CseI GACGC 2 cut(s) 166, 368
Csp6I GTAC 2 cut(s) 360, 529
CviAII CATG 7 cut(s) 15, 42, 239, 260, 296, 475, 526
CviQI GTAC 2 cut(s) 360, 529
DdeI CTNAG 9 cut(s) 47, 68, 442, 497, 634, 720, 732, 856, 868
DpnI GATC 3 cut(s) 213, 702, 759
DpnII GATC 3 cut(s) 211, 700, 757
Eam1104I CTCTTC 3 cut(s) 474, 612, 789
EarI CTCTTC 3 cut(s) 474, 612, 789
Eco57I CTGAAG 2 cut(s) 440, 530
EcoRII CCWGG 1 cut(s) 406
Esp3I CGTCTC 2 cut(s) 184, 226
FaeI CATG 7 cut(s) 18, 45, 242, 263, 299, 478, 529
FaqI GGGAC 1 cut(s) 814
FatI CATG 7 cut(s) 14, 41, 238, 259, 295, 474, 525
FauI CCCGC 1 cut(s) 139
FbaI TGATCA 2 cut(s) 211, 700
FblI GTMKAC 2 cut(s) 230, 447
Fnu4HI GCNGC 3 cut(s) 537, 558, 684
FokI GGATG 2 cut(s) 482, 827
Fsp4HI GCNGC 3 cut(s) 537, 558, 684
FspBI CTAG 2 cut(s) 614, 645
GlaI GCGC 2 cut(s) 145, 365
GluI GCNGC 3 cut(s) 537, 558, 684
GsuI CTGGAG 1 cut(s) 166
HaeIII GGCC 1 cut(s) 825
HgaI GACGC 2 cut(s) 166, 368
HhaI GCGC 2 cut(s) 146, 366
Hin1I GRCGYC 2 cut(s) 177, 379
Hin1II CATG 7 cut(s) 18, 45, 242, 263, 299, 478, 529
Hin6I GCGC 2 cut(s) 144, 364
HinP1I GCGC 2 cut(s) 144, 364
HindIII AAGCTT 3 cut(s) 251, 771, 853
HinfI GANTC 2 cut(s) 398, 414
HphI GGTGA 2 cut(s) 260, 754
Hpy166II GTNNAC 3 cut(s) 231, 448, 902
Hpy188I TCNGA 4 cut(s) 48, 69, 397, 723
Hpy188III TCNNGA 3 cut(s) 314, 418, 710
Hpy8I GTNNAC 3 cut(s) 231, 448, 902
HpyAV CCTTC 3 cut(s) 80, 459, 596
HpyCH4III ACNGT 3 cut(s) 359, 899, 906
HpyCH4IV ACGT 1 cut(s) 117
HpyCH4V TGCA 3 cut(s) 299, 543, 755
HpyF10VI GCNNNNNNNGC 4 cut(s) 183, 317, 557, 629
HpyF3I CTNAG 9 cut(s) 47, 68, 442, 497, 634, 720, 732, 856, 868
HpySE526I ACGT 1 cut(s) 117
Hsp92I GRCGYC 2 cut(s) 177, 379
Hsp92II CATG 7 cut(s) 18, 45, 242, 263, 299, 478, 529
HspAI GCGC 2 cut(s) 144, 364
Ksp22I TGATCA 2 cut(s) 211, 700
Kzo9I GATC 3 cut(s) 211, 700, 757
LmnI GCTCC 3 cut(s) 247, 325, 505
LpnPI CCDG 8 cut(s) 196, 247, 288, 393, 420, 431, 553, 839
Lsp1109I GCAGC 3 cut(s) 548, 569, 695
LweI GCATC 3 cut(s) 307, 401, 805
MaeI CTAG 2 cut(s) 614, 645
MaeII ACGT 1 cut(s) 117
MaeIII GTNAC 2 cut(s) 707, 742
MalI GATC 3 cut(s) 213, 702, 759
MboI GATC 3 cut(s) 211, 700, 757
MboII GAAGA 5 cut(s) 491, 580, 629, 806, 811
MfeI CAATTG 2 cut(s) 207, 573
MluCI AATT 5 cut(s) 207, 277, 573, 593, 687
MlyI GAGTC 1 cut(s) 423
MnlI CCTC 6 cut(s) 76, 475, 613, 717, 733, 775
MroXI GAANNNNTTC 2 cut(s) 59, 402
MseI TTAA 1 cut(s) 776
MslI CAYNNNNRTG 2 cut(s) 13, 40
MspA1I CMGCKG 1 cut(s) 698
MspR9I CCNGG 1 cut(s) 408
MunI CAATTG 2 cut(s) 207, 573
MvaI CCWGG 1 cut(s) 408
MvnI CGCG 2 cut(s) 146, 364
MwoI GCNNNNNNNGC 4 cut(s) 183, 317, 557, 629
NdeII GATC 3 cut(s) 211, 700, 757
NlaIII CATG 7 cut(s) 18, 45, 242, 263, 299, 478, 529
NlaIV GGNNCC 1 cut(s) 429
NmuCI GTSAC 2 cut(s) 707, 742
NspI RCATGY 1 cut(s) 529
PciI ACATGT 1 cut(s) 525
PcsI WCGNNNNNNNCGW 2 cut(s) 174, 376
PdmI GAANNNNTTC 2 cut(s) 59, 402
PfeI GAWTC 1 cut(s) 398
PkrI GCNGC 3 cut(s) 538, 559, 685
PleI GAGTC 1 cut(s) 422
PpsI GAGTC 1 cut(s) 422
PscI ACATGT 1 cut(s) 525
Psp1406I AACGTT 1 cut(s) 117
Psp6I CCWGG 1 cut(s) 406
PspGI CCWGG 1 cut(s) 406
PspN4I GGNNCC 1 cut(s) 429
PvuII CAGCTG 1 cut(s) 698
RsaI GTAC 2 cut(s) 361, 530
RsaNI GTAC 2 cut(s) 360, 529
RseI CAYNNNNRTG 2 cut(s) 13, 40
SaqAI TTAA 1 cut(s) 776
SatI GCNGC 3 cut(s) 537, 558, 684
Sau3AI GATC 3 cut(s) 211, 700, 757
SchI GAGTC 1 cut(s) 423
ScrFI CCNGG 1 cut(s) 408
SfaNI GCATC 3 cut(s) 307, 401, 805
SmiMI CAYNNNNRTG 2 cut(s) 13, 40
Sse9I AATT 5 cut(s) 207, 277, 573, 593, 687
SsiI CCGC 1 cut(s) 146
SspI AATATT 1 cut(s) 765
SspMI CTAG 2 cut(s) 614, 645
StyD4I CCNGG 1 cut(s) 406
TaaI ACNGT 3 cut(s) 359, 899, 906
TaiI ACGT 1 cut(s) 120
TaqI TCGA 1 cut(s) 168
TaqII GACCGA 1 cut(s) 216
TasI AATT 5 cut(s) 207, 277, 573, 593, 687
TfiI GAWTC 1 cut(s) 398
Tru1I TTAA 1 cut(s) 776
Tru9I TTAA 1 cut(s) 776
TscAI CASTG 4 cut(s) 165, 558, 757, 909
TseFI GTSAC 2 cut(s) 707, 742
TseI GCWGC 3 cut(s) 536, 557, 683
Tsp45I GTSAC 2 cut(s) 707, 742
TspDTI ATGAA 5 cut(s) 30, 284, 503, 807, 851
TspRI CASTG 4 cut(s) 165, 558, 757, 909
XceI RCATGY 1 cut(s) 529
XmiI GTMKAC 2 cut(s) 230, 447
XmnI GAANNNNTTC 2 cut(s) 59, 402
XspI CTAG 2 cut(s) 614, 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.