Rmu_sc0002729.1_g000005

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002729.1
OG0014277
Physical Location & Seq
Forward (+)
23239 .. 25807
2569 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002729.1_g000005.1.cds

Sequence Viewer

Length: 1359 bp
atgtttggatcaaagaataacatgttttctgtagactcatttaacttgaaggaaggaggaggagggaggggggagagagtgatgtggaattcgaatagcagcaatagtggaaaaagaagacaatacaatggggggccaatagcagtaggagtatcaggagacaaagccagccaaaatgctttgagatgggccattgataatcttgtcactcctccaagcgacgatgttgttgtcaagcttatccatgtcgctcaaggatcaccatgtcccattgtctcccctgggaacagtggaatgattaatgaagggcaagcttttgatcctattgaggatattttacttccctttcgttgctattgtacacgcaaacgagtaccatatgagattgttatactcaaggatcatgatgtagccaaggcactgatcgattacatttcccgccgtggcatcgggaatttaattcttggtgcttcatccaggaatgggttttctaagagactattcaagacatcagatattccaagtagtgtgcaaaagttggcaccggatttctgcaatgtatttactatcagcaaaggaaagatatgtgcagtgcgtgcagcctgccgccgagtgcctgccctgccgaccctcccggaagaatctaattacatagccgcaagcagcaaatcatatgacgaggtctcagtgcaagagcctgacatggacactggcctctcttttgtaggctcagaaagacaaagtactgatagcatgttgtattctttctacgaaaactttgggcaagctgaattgacccaaatctcttctatgtccttgaacgtggacatcactgaatcagcatcagaatcagatttcgggagtttcgatggccttaacactttgcatgaaatgccatcagcggagcagcacaatggctcaggaaatccattcttgtcgcagaaagtactggaagagatggaagaagagatgaaaagtcttagaatggagctgaagcaaacgatagagatgtaccatgcagcctataaagaagcactaacagcaaagcagaaggctacagagctccaagaggggaaaatgaaagaagaacagagactagaggaagcactactcaccttagaagcaactacagcagctttgaagaacgaaaaagaaaagtctaaggcagcgatattggcagcagaagcagctcagaggcttgttgaggaaggggtgcaaaagagagtgaatgctgacaagatggaggagaacaaggttttggatacttttggccagttctttttagctctcaagtaccaaagcttatttcatatcgtagttgctttgattttattctatgtgtacttctcaatcttttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

452

Amino Acids

50.01

Weight (kDa)

5.44

Isoelectric Point (pI)

53.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014277)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20310
malus_domestica MD13G1232700.v1.1 MD13G1233300.v1.1 MD16G1238300.v1.1
prunus_persica Prupe.1G064200_v2.0.a1
pyrus_communis pycom13g20550
rosa_chinensis RchiOBHm_Chr4g0397631
rosa_laevigata RLG00000009422
rosa_multiflora Rmu_co8218642.1_g000001 Rmu_sc0002729.1_g000005
rosa_roxburghii Rroxscaffold_5G00342440
rosa_rugosa Rorug03G0342200
rosa_samantha Rh4AG079500 Rh4BG077500 Rh4CG085400 Rh4DG072500
rosa_wichuraiana Rw4G006510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 541
AccI GTMKAC 1 cut(s) 33
AciI CCGC 4 cut(s) 439, 607, 657, 902
AclWI GGATC 4 cut(s) 16, 265, 314, 408
AcoI YGGCCR 1 cut(s) 1270
AcsI RAATTY 2 cut(s) 88, 454
AcuI CTGAAG 1 cut(s) 1013
AfaI GTAC 7 cut(s) 361, 375, 745, 948, 1013, 1295, 1343
AfiI CCNNNNNNNGG 1 cut(s) 483
AflIII ACRYGT 1 cut(s) 21
AgsI TTSAA 4 cut(s) 49, 505, 820, 1141
AjnI CCWGG 2 cut(s) 280, 476
AjuI GAANNNNNNNTTGG 4 cut(s) 1241, 1273, 1290, 1322
AluBI AGCT 9 cut(s) 238, 314, 788, 991, 1063, 1136, 1190, 1286, 1302
AluI AGCT 9 cut(s) 238, 314, 788, 991, 1063, 1136, 1190, 1286, 1302
Alw21I GWGCWC 1 cut(s) 1065
Alw26I GTCTC 5 cut(s) 153, 280, 490, 688, 1087
AlwI GGATC 4 cut(s) 16, 265, 314, 408
AoxI GGCC 5 cut(s) 134, 189, 712, 871, 1270
ApeKI GCWGC 9 cut(s) 99, 599, 663, 907, 1019, 1133, 1166, 1178, 1187
ApoI RAATTY 2 cut(s) 88, 454
AseI ATTAAT 1 cut(s) 300
AspS9I GGNCC 2 cut(s) 134, 189
AsuC2I CCSGG 1 cut(s) 635
AsuHPI GGTGA 2 cut(s) 252, 1105
AsuII TTCGAA 1 cut(s) 92
BalI TGGCCA 1 cut(s) 1272
BanI GGYRCC 1 cut(s) 541
BanII GRGCYC 1 cut(s) 1065
BbsI GAAGAC 1 cut(s) 124
Bbv12I GWGCWC 1 cut(s) 1065
BbvI GCAGC 9 cut(s) 111, 611, 675, 919, 1031, 1145, 1178, 1190, 1199
BccI CCATC 5 cut(s) 180, 863, 904, 952, 1234
BceAI ACGGC 1 cut(s) 426
BciT130I CCWGG 2 cut(s) 282, 478
BciVI GTATCC 1 cut(s) 1255
BcnI CCSGG 1 cut(s) 635
BcoDI GTCTC 5 cut(s) 153, 280, 490, 688, 1087
BfaI CTAG 1 cut(s) 1097
BfmI CTRYAG 3 cut(s) 30, 1056, 1128
BfuI GTATCC 1 cut(s) 1255
BmcAI AGTACT 2 cut(s) 745, 948
Bme1390I CCNGG 3 cut(s) 282, 478, 635
BmgT120I GGNCC 2 cut(s) 134, 189
BmiI GGNNCC 2 cut(s) 135, 543
BmrFI CCNGG 3 cut(s) 282, 478, 635
BmsI GCATC 2 cut(s) 456, 851
BpiI GAAGAC 1 cut(s) 124
Bpu10I CCTNAGC 1 cut(s) 919
Bpu14I TTCGAA 1 cut(s) 92
BpuEI CTTGAG 3 cut(s) 237, 380, 1274
BpuMI CCSGG 1 cut(s) 635
Bsa29I ATCGAT 1 cut(s) 426
BsaI GGTCTC 1 cut(s) 688
BsaJI CCNNGG 4 cut(s) 280, 281, 414, 442
BsaWI WCCGGW 1 cut(s) 544
Bsc4I CCNNNNNNNGG 1 cut(s) 483
Bse1I ACTGG 3 cut(s) 715, 954, 1273
Bse3DI GCAATG 1 cut(s) 562
BseBI CCWGG 2 cut(s) 282, 478
BseCI ATCGAT 1 cut(s) 426
BseDI CCNNGG 4 cut(s) 280, 281, 414, 442
BseGI GGATG 1 cut(s) 473
BseLI CCNNNNNNNGG 1 cut(s) 483
BseMI GCAATG 1 cut(s) 562
BseMII CTCAG 4 cut(s) 699, 744, 933, 1205
BseNI ACTGG 3 cut(s) 715, 954, 1273
BseRI GAGGAG 4 cut(s) 72, 75, 201, 1259
BseXI GCAGC 9 cut(s) 111, 611, 675, 919, 1031, 1145, 1178, 1190, 1199
BsgI GTGCAG 2 cut(s) 609, 618
BshFI GGCC 5 cut(s) 136, 191, 714, 873, 1272
BshNI GGYRCC 1 cut(s) 541
BshVI ATCGAT 1 cut(s) 426
BsiHKAI GWGCWC 1 cut(s) 1065
BsiSI CCGG 2 cut(s) 545, 635
BslFI GGGAC 1 cut(s) 252
BslI CCNNNNNNNGG 1 cut(s) 483
BsmAI GTCTC 5 cut(s) 153, 280, 490, 688, 1087
BsmFI GGGAC 1 cut(s) 252
BsmI GAATGC 1 cut(s) 1234
BsnI GGCC 5 cut(s) 136, 191, 714, 873, 1272
Bso31I GGTCTC 1 cut(s) 688
Bsp119I TTCGAA 1 cut(s) 92
Bsp1286I GDGCHC 1 cut(s) 1065
Bsp1407I TGTACA 1 cut(s) 359
Bsp143I GATC 5 cut(s) 8, 257, 319, 400, 423
BspACI CCGC 4 cut(s) 439, 607, 657, 902
BspANI GGCC 5 cut(s) 136, 191, 714, 873, 1272
BspCNI CTCAG 4 cut(s) 698, 743, 932, 1204
BspDI ATCGAT 1 cut(s) 426
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 2 cut(s) 135, 543
BspPI GGATC 4 cut(s) 16, 265, 314, 408
BspT104I TTCGAA 1 cut(s) 92
BspT107I GGYRCC 1 cut(s) 541
BspTNI GGTCTC 1 cut(s) 688
BsrDI GCAATG 1 cut(s) 562
BsrGI TGTACA 1 cut(s) 359
BsrI ACTGG 3 cut(s) 715, 954, 1273
BssECI CCNNGG 4 cut(s) 280, 281, 414, 442
BssMI GATC 5 cut(s) 8, 257, 319, 400, 423
BssT1I CCWWGG 1 cut(s) 414
Bst2UI CCWGG 2 cut(s) 282, 478
Bst4CI ACNGT 1 cut(s) 290
Bst6I CTCTTC 3 cut(s) 811, 948, 960
BstAPI GCANNNNNTGC 2 cut(s) 596, 892
BstAUI TGTACA 1 cut(s) 359
BstBI TTCGAA 1 cut(s) 92
BstC8I GCNNGC 7 cut(s) 169, 312, 597, 604, 618, 661, 786
BstDEI CTNAG 8 cut(s) 492, 685, 730, 919, 980, 1117, 1161, 1191
BstDSI CCRYGG 1 cut(s) 442
BstF5I GGATG 1 cut(s) 473
BstKTI GATC 5 cut(s) 11, 260, 322, 403, 426
BstMAI GTCTC 5 cut(s) 153, 280, 490, 688, 1087
BstMBI GATC 5 cut(s) 8, 257, 319, 400, 423
BstMWI GCNNNNNNNGC 8 cut(s) 596, 622, 892, 1040, 1130, 1175, 1184, 1187
BstNI CCWGG 2 cut(s) 282, 478
BstNSI RCATGY 2 cut(s) 25, 757
BstSCI CCNGG 3 cut(s) 280, 476, 633
BstSFI CTRYAG 3 cut(s) 30, 1056, 1128
BstV1I GCAGC 9 cut(s) 111, 611, 675, 919, 1031, 1145, 1178, 1190, 1199
BstV2I GAAGAC 1 cut(s) 124
Bsu15I ATCGAT 1 cut(s) 426
BsuI GTATCC 1 cut(s) 1255
BsuRI GGCC 5 cut(s) 136, 191, 714, 873, 1272
BsuTUI ATCGAT 1 cut(s) 426
BtgI CCRYGG 1 cut(s) 442
BtsCI GGATG 1 cut(s) 473
BtsI GCAGTG 1 cut(s) 597
BtsIMutI CAGTG 6 cut(s) 295, 419, 597, 693, 708, 831
Cac8I GCNNGC 7 cut(s) 169, 312, 597, 604, 618, 661, 786
CciI TCATGA 1 cut(s) 403
Cfr13I GGNCC 2 cut(s) 134, 189
ClaI ATCGAT 1 cut(s) 426
Csp6I GTAC 7 cut(s) 360, 374, 744, 947, 1012, 1294, 1342
CviAII CATG 8 cut(s) 22, 245, 264, 404, 703, 754, 887, 1016
CviQI GTAC 7 cut(s) 360, 374, 744, 947, 1012, 1294, 1342
DdeI CTNAG 8 cut(s) 492, 685, 730, 919, 980, 1117, 1161, 1191
DpnI GATC 5 cut(s) 10, 259, 321, 402, 425
DpnII GATC 5 cut(s) 8, 257, 319, 400, 423
EaeI YGGCCR 1 cut(s) 1270
Eam1104I CTCTTC 3 cut(s) 811, 948, 960
EarI CTCTTC 3 cut(s) 811, 948, 960
Ecl136II GAGCTC 1 cut(s) 1063
Eco130I CCWWGG 1 cut(s) 414
Eco24I GRGCYC 1 cut(s) 1065
Eco31I GGTCTC 1 cut(s) 688
Eco53kI GAGCTC 1 cut(s) 1063
Eco57I CTGAAG 1 cut(s) 1013
EcoICRI GAGCTC 1 cut(s) 1063
EcoRI GAATTC 1 cut(s) 88
EcoRII CCWGG 2 cut(s) 280, 476
EcoT14I CCWWGG 1 cut(s) 414
EcoT38I GRGCYC 1 cut(s) 1065
ErhI CCWWGG 1 cut(s) 414
FaeI CATG 8 cut(s) 25, 248, 267, 407, 706, 757, 890, 1019
FaqI GGGAC 1 cut(s) 252
FatI CATG 8 cut(s) 21, 244, 263, 403, 702, 753, 886, 1015
FauI CCCGC 1 cut(s) 446
FauNDI CATATG 2 cut(s) 379, 673
FblI GTMKAC 1 cut(s) 33
FokI GGATG 1 cut(s) 460
FriOI GRGCYC 1 cut(s) 1065
FspBI CTAG 1 cut(s) 1097
HaeIII GGCC 5 cut(s) 136, 191, 714, 873, 1272
HapII CCGG 2 cut(s) 545, 635
Hin1II CATG 8 cut(s) 25, 248, 267, 407, 706, 757, 890, 1019
HindIII AAGCTT 3 cut(s) 236, 312, 1300
HinfI GANTC 4 cut(s) 35, 641, 836, 848
HpaII CCGG 2 cut(s) 545, 635
HphI GGTGA 2 cut(s) 252, 1105
Hpy166II GTNNAC 4 cut(s) 34, 362, 826, 1342
Hpy188I TCNGA 5 cut(s) 514, 733, 847, 853, 1194
Hpy188III TCNNGA 6 cut(s) 156, 404, 451, 505, 859, 921
Hpy8I GTNNAC 4 cut(s) 34, 362, 826, 1342
Hpy99I CGWCG 1 cut(s) 224
HpyAV CCTTC 5 cut(s) 43, 47, 299, 1045, 1202
HpyCH4III ACNGT 1 cut(s) 290
HpyCH4IV ACGT 1 cut(s) 822
HpyCH4V TGCA 8 cut(s) 532, 555, 590, 599, 691, 886, 1019, 1216
HpyF10VI GCNNNNNNNGC 8 cut(s) 596, 622, 892, 1040, 1130, 1175, 1184, 1187
HpyF3I CTNAG 8 cut(s) 492, 685, 730, 919, 980, 1117, 1161, 1191
HpySE526I ACGT 1 cut(s) 822
Hsp92II CATG 8 cut(s) 25, 248, 267, 407, 706, 757, 890, 1019
Kzo9I GATC 5 cut(s) 8, 257, 319, 400, 423
LmnI GCTCC 3 cut(s) 904, 988, 1068
Lsp1109I GCAGC 9 cut(s) 111, 611, 675, 919, 1031, 1145, 1178, 1190, 1199
LweI GCATC 2 cut(s) 456, 851
MaeI CTAG 1 cut(s) 1097
MaeII ACGT 1 cut(s) 822
MaeIII GTNAC 1 cut(s) 205
MalI GATC 5 cut(s) 10, 259, 321, 402, 425
MboI GATC 5 cut(s) 8, 257, 319, 400, 423
MboII GAAGA 8 cut(s) 129, 650, 798, 965, 974, 977, 1097, 1153
MhlI GDGCHC 1 cut(s) 1065
MlsI TGGCCA 1 cut(s) 1272
MluCI AATT 5 cut(s) 88, 454, 459, 646, 791
MluNI TGGCCA 1 cut(s) 1272
MlyI GAGTC 1 cut(s) 29
Mox20I TGGCCA 1 cut(s) 1272
MscI TGGCCA 1 cut(s) 1272
MseI TTAA 4 cut(s) 42, 300, 458, 876
Msp20I TGGCCA 1 cut(s) 1272
MspA1I CMGCKG 1 cut(s) 902
MspI CCGG 2 cut(s) 545, 635
MspR9I CCNGG 3 cut(s) 282, 478, 635
Mva1269I GAATGC 1 cut(s) 1234
MvaI CCWGG 2 cut(s) 282, 478
MwoI GCNNNNNNNGC 8 cut(s) 596, 622, 892, 1040, 1130, 1175, 1184, 1187
NciI CCSGG 1 cut(s) 635
NdeI CATATG 2 cut(s) 379, 673
NdeII GATC 5 cut(s) 8, 257, 319, 400, 423
NlaIII CATG 8 cut(s) 25, 248, 267, 407, 706, 757, 890, 1019
NlaIV GGNNCC 2 cut(s) 135, 543
NmeAIII GCCGAG 1 cut(s) 635
NmuCI GTSAC 1 cut(s) 205
NspI RCATGY 2 cut(s) 25, 757
NspV TTCGAA 1 cut(s) 92
PagI TCATGA 1 cut(s) 403
PasI CCCWGGG 1 cut(s) 281
PciI ACATGT 1 cut(s) 21
PcsI WCGNNNNNNNCGW 1 cut(s) 864
PctI GAATGC 1 cut(s) 1234
PfeI GAWTC 3 cut(s) 641, 836, 848
PflFI GACNNNGTC 1 cut(s) 680
PfoI TCCNGGA 2 cut(s) 476, 633
PleI GAGTC 1 cut(s) 29
PpsI GAGTC 1 cut(s) 29
PscI ACATGT 1 cut(s) 21
PshBI ATTAAT 1 cut(s) 300
Psp124BI GAGCTC 1 cut(s) 1065
Psp6I CCWGG 2 cut(s) 280, 476
PspGI CCWGG 2 cut(s) 280, 476
PspN4I GGNNCC 2 cut(s) 135, 543
PspPI GGNCC 2 cut(s) 134, 189
PsyI GACNNNGTC 1 cut(s) 680
RsaI GTAC 7 cut(s) 361, 375, 745, 948, 1013, 1295, 1343
RsaNI GTAC 7 cut(s) 360, 374, 744, 947, 1012, 1294, 1342
SacI GAGCTC 1 cut(s) 1065
SaqAI TTAA 4 cut(s) 42, 300, 458, 876
Sau3AI GATC 5 cut(s) 8, 257, 319, 400, 423
Sau96I GGNCC 2 cut(s) 134, 189
ScaI AGTACT 2 cut(s) 745, 948
SchI GAGTC 1 cut(s) 29
ScrFI CCNGG 3 cut(s) 282, 478, 635
SduI GDGCHC 1 cut(s) 1065
SfaNI GCATC 2 cut(s) 456, 851
SfcI CTRYAG 3 cut(s) 30, 1056, 1128
SfuI TTCGAA 1 cut(s) 92
SmlI CTYRAG 3 cut(s) 252, 395, 1289
SmoI CTYRAG 3 cut(s) 252, 395, 1289
Sse9I AATT 5 cut(s) 88, 454, 459, 646, 791
SsiI CCGC 4 cut(s) 439, 607, 657, 902
SspMI CTAG 1 cut(s) 1097
SstI GAGCTC 1 cut(s) 1065
StyD4I CCNGG 3 cut(s) 280, 476, 633
StyI CCWWGG 1 cut(s) 414
TaaI ACNGT 1 cut(s) 290
TaiI ACGT 1 cut(s) 825
TaqI TCGA 3 cut(s) 92, 426, 867
TasI AATT 5 cut(s) 88, 454, 459, 646, 791
TatI WGTACW 4 cut(s) 359, 743, 946, 1341
TauI GCSGC 2 cut(s) 609, 659
TfiI GAWTC 3 cut(s) 641, 836, 848
Tru1I TTAA 4 cut(s) 42, 300, 458, 876
Tru9I TTAA 4 cut(s) 42, 300, 458, 876
TscAI CASTG 6 cut(s) 295, 426, 597, 693, 715, 838
TseFI GTSAC 1 cut(s) 205
TseI GCWGC 9 cut(s) 99, 599, 663, 907, 1019, 1133, 1166, 1178, 1187
Tsp45I GTSAC 1 cut(s) 205
TspDTI ATGAA 6 cut(s) 318, 462, 903, 986, 1094, 1298
TspRI CASTG 6 cut(s) 295, 426, 597, 693, 715, 838
Tth111I GACNNNGTC 1 cut(s) 680
VspI ATTAAT 1 cut(s) 300
XapI RAATTY 2 cut(s) 88, 454
XceI RCATGY 2 cut(s) 25, 757
XmiI GTMKAC 1 cut(s) 33
XspI CTAG 1 cut(s) 1097
ZrmI AGTACT 2 cut(s) 745, 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.