MD16G1238300.v1.1

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014277
Physical Location & Seq
Forward (+)
25137994 .. 25142627
4634 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1238300.v1.1.491

Sequence Viewer

Length: 1398 bp
ATGTTCCCATATTCATATTTGAGCAAGAGGTTGTTGAATCTGAGAGTTGAGAAAGAGGAAGGGCGGAAGAGAATAATGTGGTATAACAATGTTGATAATGGAAATGATAATCAAGGCGCGGGGACAATAGCAGTGGCAATCGGCGAGGGTGTCTCCAGCCAAAATGCTTTGAAATGGGCTATTGATCATCTCGTCTCTAGATCTACCACCCATGCTCCTATCAAGCTTGTCCATGTTATTCACCAATCTAATTCCTATAATAAACTTCATGCAACTGAGAACAACCCATCAAATGAAGGGCAAGCTCATGATGCTCCAAATACAGATTTCTTGCTTCCATTTCGTTGCTACTGTACACGCAAACGAGTACAATGCGAGACTGTTCTACTGGAGGATCAAGATGTAGCTGAGGCCTTAGTTGAGTACATTTCCCGCCATGGCATTGTGAGTATTGTTGTTGGTGCGACATCCAGGACTGGTGGGTTAACAAGGCGTCGATTCAAGGCATCTGAATCTATTCCAGGCAGAGTCCTGAAGTTGGCACCACATTTCTGCTCAGTATACACCATCAAACAAGGAAGGGTGAGTGAAATGCGTGAAGCGTCTCGTCCACTTCCAAATATTCCTGCTGGGGAGAGGGCAGCAGATCACCATTCCGATCACATAGGTAGAGGTCCGATCAACGGAGCATATGATGAGGTCTCAATGGCTGAGAATGATGTTTCGTTTGTAAGCTCAGAAAGACCAAGTACTGATAGCATTTTATCTTTCTACCAAAATTTTGGGCCAGCTGGACTGACCCGATTTCCTTTAGAATCATCATCAAACATGGAGAGTATTGCAAAACTTGACGAAGATTTTGACAGTTTTGATTTTTATGCTCCCCATGAACCGTCATCAACTGTACAATGTTGTGGAGGAACACCATTCTTGTCGCAGAAAGTATTGGAAGATATGGAAGAGGAGATGAAAAGGCTTAGAGTGGAGCTGAAGCAAACAATGGACATGTACCATGCAGCCTGCAAAGAAGCAGTGGCAGCAAAACAGAAGACAATTGAGCTAGAGGAGTGGAAACTGAAAGAAGAGAAAAAACTAGAAGAGGCACGACTTGCCTTAGAAGCAACTAGGGCAGCTATGGAGAAGAAAAAAGCAAATTCTAAGGCAGCAGTTTTGGCAGCTGATCATCATCACGAAGCCGCTCAGAGGTTTACTGAGAAAGAGGTGCAAACAACGATCAGTGCAGATCAAATATTCAAAGCTTTAAGGGAAGCTGACGATGAAGAAAAGAAAAGAGTTTTGGATGCTTTAGGCCAGTCCCAGATAGTTTTCAAATACCAAAGCTTAGTTCATATCTTAGTTGTTTTATTGTTACTTTCTTTTTACTGTTCACTGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

466

Amino Acids

52.22

Weight (kDa)

6.21

Isoelectric Point (pI)

47.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 42 - 161 4e-09 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014277)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20310
malus_domestica MD13G1232700.v1.1 MD13G1233300.v1.1 MD16G1238300.v1.1
prunus_persica Prupe.1G064200_v2.0.a1
pyrus_communis pycom13g20550
rosa_chinensis RchiOBHm_Chr4g0397631
rosa_laevigata RLG00000009422
rosa_multiflora Rmu_co8218642.1_g000001 Rmu_sc0002729.1_g000005
rosa_roxburghii Rroxscaffold_5G00342440
rosa_rugosa Rorug03G0342200
rosa_samantha Rh4AG079500 Rh4BG077500 Rh4CG085400 Rh4DG072500
rosa_wichuraiana Rw4G006510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 541
AccBSI CCGCTC 1 cut(s) 1199
AccI GTMKAC 1 cut(s) 561
AccII CGCG 1 cut(s) 119
AciI CCGC 4 cut(s) 64, 119, 433, 1197
AclWI GGATC 1 cut(s) 402
AcsI RAATTY 2 cut(s) 778, 1153
AcuI CTGAAG 2 cut(s) 554, 1010
AcyI GRCGYC 1 cut(s) 493
AfaI GTAC 6 cut(s) 355, 369, 425, 751, 906, 1010
AfiI CCNNNNNNNGG 3 cut(s) 538, 683, 1203
AflIII ACRYGT 1 cut(s) 1005
AgsI TTSAA 5 cut(s) 37, 172, 502, 1255, 1330
AjnI CCWGG 2 cut(s) 470, 520
AjuI GAANNNNNNNTTGG 4 cut(s) 1280, 1312, 1329, 1361
Alw26I GTCTC 5 cut(s) 157, 199, 371, 609, 706
AlwI GGATC 1 cut(s) 402
AoxI GGCC 3 cut(s) 411, 785, 1309
ApeKI GCWGC 6 cut(s) 641, 1016, 1037, 1130, 1163, 1175
ApoI RAATTY 2 cut(s) 778, 1153
ArsI GACNNNNNNTTYG 2 cut(s) 842, 874
Asp700I GAANNNNTTC 1 cut(s) 516
AspLEI GCGC 1 cut(s) 119
AspS9I GGNCC 2 cut(s) 674, 785
AsuHPI GGTGA 3 cut(s) 233, 595, 641
AvaII GGWCC 1 cut(s) 674
BanI GGYRCC 1 cut(s) 541
BbsI GAAGAC 1 cut(s) 1055
BbvCI CCTCAGC 1 cut(s) 408
BbvI GCAGC 6 cut(s) 653, 1028, 1049, 1142, 1175, 1187
BccI CCATC 2 cut(s) 295, 575
BcgI CGANNNNNNTGC 2 cut(s) 354, 388
BciT130I CCWGG 2 cut(s) 472, 522
BclI TGATCA 2 cut(s) 184, 1180
BcoDI GTCTC 5 cut(s) 157, 199, 371, 609, 706
BfaI CTAG 4 cut(s) 198, 1061, 1094, 1125
BglII AGATCT 1 cut(s) 200
BisI GCNGC 7 cut(s) 642, 1017, 1038, 1131, 1164, 1176, 1197
BlsI GCNGC 7 cut(s) 643, 1018, 1039, 1132, 1165, 1177, 1198
BmcAI AGTACT 1 cut(s) 751
Bme1390I CCNGG 2 cut(s) 472, 522
Bme18I GGWCC 1 cut(s) 674
BmgT120I GGNCC 2 cut(s) 674, 785
BmiI GGNNCC 1 cut(s) 543
BmrFI CCNGG 2 cut(s) 472, 522
BmsI GCATC 3 cut(s) 301, 515, 1291
BpiI GAAGAC 1 cut(s) 1055
BplI GAGNNNNNCTC 2 cut(s) 137, 169
BpmI CTGGAG 2 cut(s) 139, 410
Bpu10I CCTNAGC 1 cut(s) 408
BsaBI GATNNNNATC 1 cut(s) 1185
BsaHI GRCGYC 1 cut(s) 493
BsaI GGTCTC 1 cut(s) 706
BsaJI CCNNGG 1 cut(s) 436
BsaXI ACNNNNNCTCC 2 cut(s) 199, 229
Bsc4I CCNNNNNNNGG 3 cut(s) 538, 683, 1203
Bse1I ACTGG 3 cut(s) 393, 481, 1312
Bse8I GATNNNNATC 1 cut(s) 1185
BseBI CCWGG 2 cut(s) 472, 522
BseDI CCNNGG 1 cut(s) 436
BseGI GGATG 2 cut(s) 467, 1306
BseJI GATNNNNATC 1 cut(s) 1185
BseLI CCNNNNNNNGG 3 cut(s) 538, 683, 1203
BseMII CTCAG 8 cut(s) 32, 267, 399, 570, 702, 750, 1203, 1214
BseNI ACTGG 3 cut(s) 393, 481, 1312
BseRI GAGGAG 2 cut(s) 977, 1079
BseXI GCAGC 6 cut(s) 653, 1028, 1049, 1142, 1175, 1187
BseYI CCCAGC 1 cut(s) 629
BsgI GTGCAG 1 cut(s) 1260
Bsh1236I CGCG 1 cut(s) 119
BshFI GGCC 3 cut(s) 413, 787, 1311
BshNI GGYRCC 1 cut(s) 541
BslFI GGGAC 2 cut(s) 136, 1300
BslI CCNNNNNNNGG 3 cut(s) 538, 683, 1203
BsmAI GTCTC 5 cut(s) 157, 199, 371, 609, 706
BsmBI CGTCTC 2 cut(s) 199, 609
BsmFI GGGAC 2 cut(s) 136, 1300
BsnI GGCC 3 cut(s) 413, 787, 1311
Bso31I GGTCTC 1 cut(s) 706
Bsp1407I TGTACA 2 cut(s) 353, 904
Bsp143I GATC 9 cut(s) 184, 200, 394, 646, 658, 678, 1180, 1233, 1243
Bsp19I CCATGG 1 cut(s) 436
BspACI CCGC 4 cut(s) 64, 119, 433, 1197
BspANI GGCC 3 cut(s) 413, 787, 1311
BspCNI CTCAG 8 cut(s) 33, 268, 400, 569, 703, 749, 1204, 1213
BspFNI CGCG 1 cut(s) 119
BspHI TCATGA 1 cut(s) 307
BspLI GGNNCC 1 cut(s) 543
BspPI GGATC 1 cut(s) 402
BspT107I GGYRCC 1 cut(s) 541
BspTNI GGTCTC 1 cut(s) 706
BsrBI CCGCTC 1 cut(s) 1199
BsrGI TGTACA 2 cut(s) 353, 904
BsrI ACTGG 3 cut(s) 393, 481, 1312
BssECI CCNNGG 1 cut(s) 436
BssMI GATC 9 cut(s) 184, 200, 394, 646, 658, 678, 1180, 1233, 1243
BssNAI GTATAC 1 cut(s) 562
BssNI GRCGYC 1 cut(s) 493
BssT1I CCWWGG 1 cut(s) 436
Bst1107I GTATAC 1 cut(s) 562
Bst2UI CCWGG 2 cut(s) 472, 522
Bst4CI ACNGT 7 cut(s) 353, 382, 866, 894, 904, 1385, 1392
Bst6I CTCTTC 4 cut(s) 62, 954, 1077, 1092
BstACI GRCGYC 1 cut(s) 493
BstAPI GCANNNNNTGC 1 cut(s) 1109
BstAUI TGTACA 2 cut(s) 353, 904
BstC8I GCNNGC 3 cut(s) 303, 789, 1021
BstDSI CCRYGG 1 cut(s) 436
BstF5I GGATG 2 cut(s) 467, 1306
BstFNI CGCG 1 cut(s) 119
BstHHI GCGC 1 cut(s) 119
BstKTI GATC 9 cut(s) 187, 203, 397, 649, 661, 681, 1183, 1236, 1246
BstMAI GTCTC 5 cut(s) 157, 199, 371, 609, 706
BstMBI GATC 9 cut(s) 184, 200, 394, 646, 658, 678, 1180, 1233, 1243
BstMWI GCNNNNNNNGC 6 cut(s) 311, 1037, 1109, 1118, 1127, 1172
BstNI CCWGG 2 cut(s) 472, 522
BstNSI RCATGY 1 cut(s) 1009
BstSCI CCNGG 2 cut(s) 470, 520
BstUI CGCG 1 cut(s) 119
BstV1I GCAGC 6 cut(s) 653, 1028, 1049, 1142, 1175, 1187
BstV2I GAAGAC 1 cut(s) 1055
BstX2I RGATCY 1 cut(s) 200
BstXI CCANNNNNNTGG 1 cut(s) 782
BstYI RGATCY 1 cut(s) 200
BstZ17I GTATAC 1 cut(s) 562
BsuRI GGCC 3 cut(s) 413, 787, 1311
BtgI CCRYGG 1 cut(s) 436
BtsCI GGATG 2 cut(s) 467, 1306
BtsI GCAGTG 2 cut(s) 138, 1038
BtsIMutI CAGTG 4 cut(s) 138, 1038, 1243, 1388
Cac8I GCNNGC 3 cut(s) 303, 789, 1021
CciI TCATGA 1 cut(s) 307
CfoI GCGC 1 cut(s) 119
Cfr13I GGNCC 2 cut(s) 674, 785
CseI GACGC 2 cut(s) 482, 591
Csp6I GTAC 6 cut(s) 354, 368, 424, 750, 905, 1009
CspCI CAANNNNNGTGG 2 cut(s) 114, 149
CviAII CATG 9 cut(s) 212, 233, 269, 308, 437, 829, 887, 1006, 1013
CviQI GTAC 6 cut(s) 354, 368, 424, 750, 905, 1009
DpnI GATC 9 cut(s) 186, 202, 396, 648, 660, 680, 1182, 1235, 1245
DpnII GATC 9 cut(s) 184, 200, 394, 646, 658, 678, 1180, 1233, 1243
Eam1104I CTCTTC 4 cut(s) 62, 954, 1077, 1092
EarI CTCTTC 4 cut(s) 62, 954, 1077, 1092
EciI GGCGGA 1 cut(s) 79
Eco130I CCWWGG 1 cut(s) 436
Eco147I AGGCCT 1 cut(s) 413
Eco31I GGTCTC 1 cut(s) 706
Eco47I GGWCC 1 cut(s) 674
Eco57I CTGAAG 2 cut(s) 554, 1010
EcoRII CCWGG 2 cut(s) 470, 520
EcoT14I CCWWGG 1 cut(s) 436
ErhI CCWWGG 1 cut(s) 436
Esp3I CGTCTC 2 cut(s) 199, 609
FaeI CATG 9 cut(s) 215, 236, 272, 311, 440, 832, 890, 1009, 1016
FaqI GGGAC 2 cut(s) 136, 1300
FatI CATG 9 cut(s) 211, 232, 268, 307, 436, 828, 886, 1005, 1012
FauI CCCGC 2 cut(s) 112, 440
FauNDI CATATG 1 cut(s) 691
FbaI TGATCA 2 cut(s) 184, 1180
FblI GTMKAC 1 cut(s) 561
Fnu4HI GCNGC 7 cut(s) 642, 1017, 1038, 1131, 1164, 1176, 1197
FokI GGATG 2 cut(s) 454, 1313
Fsp4HI GCNGC 7 cut(s) 642, 1017, 1038, 1131, 1164, 1176, 1197
FspBI CTAG 4 cut(s) 198, 1061, 1094, 1125
GlaI GCGC 1 cut(s) 118
GluI GCNGC 7 cut(s) 642, 1017, 1038, 1131, 1164, 1176, 1197
GsaI CCCAGC 1 cut(s) 633
GsuI CTGGAG 2 cut(s) 139, 410
HaeIII GGCC 3 cut(s) 413, 787, 1311
HgaI GACGC 2 cut(s) 482, 591
HhaI GCGC 1 cut(s) 119
Hin1I GRCGYC 1 cut(s) 493
Hin1II CATG 9 cut(s) 215, 236, 272, 311, 440, 832, 890, 1009, 1016
Hin6I GCGC 1 cut(s) 117
HinP1I GCGC 1 cut(s) 117
HincII GTYRAC 1 cut(s) 486
HindII GTYRAC 1 cut(s) 486
HindIII AAGCTT 3 cut(s) 224, 1257, 1339
HinfI GANTC 5 cut(s) 37, 498, 512, 528, 815
HpaI GTTAAC 1 cut(s) 486
HphI GGTGA 3 cut(s) 233, 595, 641
Hpy166II GTNNAC 6 cut(s) 356, 486, 562, 611, 1209, 1388
Hpy188I TCNGA 6 cut(s) 42, 511, 658, 678, 739, 1203
Hpy188III TCNNGA 5 cut(s) 198, 308, 398, 532, 1190
Hpy8I GTNNAC 6 cut(s) 356, 486, 562, 611, 1209, 1388
Hpy99I CGWCG 1 cut(s) 498
HpyAV CCTTC 3 cut(s) 53, 290, 573
HpyCH4III ACNGT 7 cut(s) 353, 382, 866, 894, 904, 1385, 1392
HpyCH4V TGCA 6 cut(s) 272, 842, 1016, 1023, 1225, 1241
HpyF10VI GCNNNNNNNGC 6 cut(s) 311, 1037, 1109, 1118, 1127, 1172
Hsp92I GRCGYC 1 cut(s) 493
Hsp92II CATG 9 cut(s) 215, 236, 272, 311, 440, 832, 890, 1009, 1016
HspAI GCGC 1 cut(s) 117
Ksp22I TGATCA 2 cut(s) 184, 1180
KspAI GTTAAC 1 cut(s) 486
Kzo9I GATC 9 cut(s) 184, 200, 394, 646, 658, 678, 1180, 1233, 1243
LmnI GCTCC 5 cut(s) 220, 319, 686, 886, 985
Lsp1109I GCAGC 6 cut(s) 653, 1028, 1049, 1142, 1175, 1187
LweI GCATC 3 cut(s) 301, 515, 1291
MaeI CTAG 4 cut(s) 198, 1061, 1094, 1125
MaeIII GTNAC 1 cut(s) 1368
MalI GATC 9 cut(s) 186, 202, 396, 648, 660, 680, 1182, 1235, 1245
MbiI CCGCTC 1 cut(s) 1199
MboI GATC 9 cut(s) 184, 200, 394, 646, 658, 678, 1180, 1233, 1243
MboII GAAGA 9 cut(s) 79, 866, 962, 971, 1060, 1094, 1109, 1153, 1292
MfeI CAATTG 1 cut(s) 1053
MflI RGATCY 1 cut(s) 200
MluCI AATT 4 cut(s) 250, 778, 1053, 1153
MlyI GAGTC 1 cut(s) 537
MroXI GAANNNNTTC 1 cut(s) 516
MseI TTAA 2 cut(s) 485, 1262
MspA1I CMGCKG 2 cut(s) 791, 1178
MspR9I CCNGG 2 cut(s) 472, 522
MunI CAATTG 1 cut(s) 1053
MvaI CCWGG 2 cut(s) 472, 522
MvnI CGCG 1 cut(s) 119
MwoI GCNNNNNNNGC 6 cut(s) 311, 1037, 1109, 1118, 1127, 1172
NcoI CCATGG 1 cut(s) 436
NdeI CATATG 1 cut(s) 691
NdeII GATC 9 cut(s) 184, 200, 394, 646, 658, 678, 1180, 1233, 1243
NlaIII CATG 9 cut(s) 215, 236, 272, 311, 440, 832, 890, 1009, 1016
NlaIV GGNNCC 1 cut(s) 543
NspI RCATGY 1 cut(s) 1009
PagI TCATGA 1 cut(s) 307
PceI AGGCCT 1 cut(s) 413
PciI ACATGT 1 cut(s) 1005
PdmI GAANNNNTTC 1 cut(s) 516
PfeI GAWTC 4 cut(s) 37, 498, 512, 815
PfoI TCCNGGA 1 cut(s) 470
PkrI GCNGC 7 cut(s) 643, 1018, 1039, 1132, 1165, 1177, 1198
PleI GAGTC 1 cut(s) 536
PpsI GAGTC 1 cut(s) 536
PscI ACATGT 1 cut(s) 1005
Psp6I CCWGG 2 cut(s) 470, 520
PspFI CCCAGC 1 cut(s) 629
PspGI CCWGG 2 cut(s) 470, 520
PspN4I GGNNCC 1 cut(s) 543
PspPI GGNCC 2 cut(s) 674, 785
PsuI RGATCY 1 cut(s) 200
PvuII CAGCTG 2 cut(s) 791, 1178
RsaI GTAC 6 cut(s) 355, 369, 425, 751, 906, 1010
RsaNI GTAC 6 cut(s) 354, 368, 424, 750, 905, 1009
SaqAI TTAA 2 cut(s) 485, 1262
SatI GCNGC 7 cut(s) 642, 1017, 1038, 1131, 1164, 1176, 1197
Sau3AI GATC 9 cut(s) 184, 200, 394, 646, 658, 678, 1180, 1233, 1243
Sau96I GGNCC 2 cut(s) 674, 785
ScaI AGTACT 1 cut(s) 751
SchI GAGTC 1 cut(s) 537
ScrFI CCNGG 2 cut(s) 472, 522
SfaNI GCATC 3 cut(s) 301, 515, 1291
SinI GGWCC 1 cut(s) 674
Sse9I AATT 4 cut(s) 250, 778, 1053, 1153
SseBI AGGCCT 1 cut(s) 413
SsiI CCGC 4 cut(s) 64, 119, 433, 1197
SspI AATATT 2 cut(s) 622, 1251
SspMI CTAG 4 cut(s) 198, 1061, 1094, 1125
StuI AGGCCT 1 cut(s) 413
StyD4I CCNGG 2 cut(s) 470, 520
StyI CCWWGG 1 cut(s) 436
TaaI ACNGT 7 cut(s) 353, 382, 866, 894, 904, 1385, 1392
TaqI TCGA 1 cut(s) 496
TasI AATT 4 cut(s) 250, 778, 1053, 1153
TatI WGTACW 5 cut(s) 353, 367, 423, 749, 904
TauI GCSGC 1 cut(s) 1199
TfiI GAWTC 4 cut(s) 37, 498, 512, 815
Tru1I TTAA 2 cut(s) 485, 1262
Tru9I TTAA 2 cut(s) 485, 1262
TscAI CASTG 4 cut(s) 138, 1038, 1243, 1395
TseI GCWGC 6 cut(s) 641, 1016, 1037, 1130, 1163, 1175
TspDTI ATGAA 6 cut(s) 257, 309, 903, 983, 1293, 1337
TspGWI ACGGA 1 cut(s) 699
TspRI CASTG 4 cut(s) 138, 1038, 1243, 1395
VpaK11BI GGWCC 1 cut(s) 674
XapI RAATTY 2 cut(s) 778, 1153
XbaI TCTAGA 1 cut(s) 197
XceI RCATGY 1 cut(s) 1009
XmiI GTMKAC 1 cut(s) 561
XmnI GAANNNNTTC 1 cut(s) 516
XspI CTAG 4 cut(s) 198, 1061, 1094, 1125
ZrmI AGTACT 1 cut(s) 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.