Rh4DG072500

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0014277
Physical Location & Seq
Forward (+)
10739176 .. 10743240
4065 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG072500.1

Sequence Viewer

Length: 768 bp
ATGTTGTTGTCAAGCTTATCCATGTCGCTCAAGGATCACCATGTCCCATTGTCTCCCCTGGTACCATTTGAGATTGTTATACTCAAGGTTCATGATGTAGCCAAGGCACTGATCGATTACATTTCCCGCCGTGGCATCGGGAATTTAATTCTTGGTGCTTCATCCAGGAATGGGTTTTCTAAGAGACTATTCAAGACATCAGATATTCCAAGTAGTGTGCAAAAGTTGGCACCGGATTTCTGCAATGTATTTACTATCAGCAAAGGAAAGATAAGTGCAGTGCGTGCAGCCCGCCGCCGAGTGCCTGCCCTGCCGGCCCTCCCGGTAGAATCTAATTTCATAGGCAGAGCCGCAAGCAGCAAATCATATGACGAGGTCTCAGTGCAAGAGCCTGACATGGACACTGGCCTCTCTTTTGTAGGCTCAGAAAGACGAAGTACTGATAGCATGTTGTATTCTTTCTACGAAAACTTTGGGCAAGCTGAATTGACCCAAATCTCTTCTATGTCCTTGAACGTGGACATCACTGAATCAGCATCAGAATCAGATTTCGGGAGTTTCGATGGCCTTAACACTTTGCATGAAATGCCATCAGCGGCGCAGCACAATGGCTCAGGAAATCCATTCTTGTCGCAGAAAGTACTGGAAGAGATGGAAGAAGAGATGAGGAGTCTTAGAATGGAGCTGAAGCAAACGATAGAGATGTACCATGCAGCCTATAAAGAAGCACTGACAGCAAAACAGAAGAGCTCCAAGAGGGGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

255

Amino Acids

28.13

Weight (kDa)

7.03

Isoelectric Point (pI)

58.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014277)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20310
malus_domestica MD13G1232700.v1.1 MD13G1233300.v1.1 MD16G1238300.v1.1
prunus_persica Prupe.1G064200_v2.0.a1
pyrus_communis pycom13g20550
rosa_chinensis RchiOBHm_Chr4g0397631
rosa_laevigata RLG00000009422
rosa_multiflora Rmu_co8218642.1_g000001 Rmu_sc0002729.1_g000005
rosa_roxburghii Rroxscaffold_5G00342440
rosa_rugosa Rorug03G0342200
rosa_samantha Rh4AG079500 Rh4BG077500 Rh4CG085400 Rh4DG072500
rosa_wichuraiana Rw4G006510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 61
AccB1I GGYRCC 2 cut(s) 61, 229
AciI CCGC 5 cut(s) 127, 292, 295, 351, 596
AclWI GGATC 1 cut(s) 42
AcsI RAATTY 1 cut(s) 142
AcuI CTGAAG 1 cut(s) 707
AfaI GTAC 4 cut(s) 63, 439, 642, 707
AfiI CCNNNNNNNGG 1 cut(s) 171
AgsI TTSAA 2 cut(s) 193, 514
AjnI CCWGG 2 cut(s) 57, 164
AluBI AGCT 4 cut(s) 15, 482, 685, 750
AluI AGCT 4 cut(s) 15, 482, 685, 750
Alw21I GWGCWC 1 cut(s) 752
Alw26I GTCTC 3 cut(s) 57, 178, 382
AlwI GGATC 1 cut(s) 42
AoxI GGCC 3 cut(s) 315, 406, 565
ApeKI GCWGC 4 cut(s) 287, 357, 601, 713
ApoI RAATTY 1 cut(s) 142
Asp718I GGTACC 1 cut(s) 61
AspLEI GCGC 1 cut(s) 601
AspS9I GGNCC 1 cut(s) 316
AsuC2I CCSGG 1 cut(s) 323
AsuHPI GGTGA 1 cut(s) 29
BanI GGYRCC 2 cut(s) 61, 229
BanII GRGCYC 1 cut(s) 752
Bbv12I GWGCWC 1 cut(s) 752
BbvI GCAGC 4 cut(s) 299, 369, 613, 725
BccI CCATC 3 cut(s) 557, 598, 646
BceAI ACGGC 1 cut(s) 114
BciT130I CCWGG 2 cut(s) 59, 166
BcnI CCSGG 1 cut(s) 323
BcoDI GTCTC 3 cut(s) 57, 178, 382
BglI GCCNNNNNGGC 1 cut(s) 314
BisI GCNGC 7 cut(s) 288, 295, 351, 358, 597, 602, 714
BlsI GCNGC 7 cut(s) 289, 296, 352, 359, 598, 603, 715
BmcAI AGTACT 2 cut(s) 439, 642
Bme1390I CCNGG 3 cut(s) 59, 166, 323
BmgT120I GGNCC 1 cut(s) 316
BmiI GGNNCC 2 cut(s) 63, 231
BmrFI CCNGG 3 cut(s) 59, 166, 323
BmsI GCATC 2 cut(s) 144, 545
Bpu10I CCTNAGC 1 cut(s) 613
BpuEI CTTGAG 2 cut(s) 14, 68
BpuMI CCSGG 1 cut(s) 323
Bsa29I ATCGAT 1 cut(s) 114
BsaI GGTCTC 1 cut(s) 382
BsaJI CCNNGG 3 cut(s) 57, 102, 130
BsaWI WCCGGW 1 cut(s) 232
Bsc4I CCNNNNNNNGG 1 cut(s) 171
Bse118I RCCGGY 1 cut(s) 313
Bse1I ACTGG 2 cut(s) 409, 648
Bse3DI GCAATG 1 cut(s) 250
BseBI CCWGG 2 cut(s) 59, 166
BseCI ATCGAT 1 cut(s) 114
BseDI CCNNGG 3 cut(s) 57, 102, 130
BseGI GGATG 1 cut(s) 161
BseLI CCNNNNNNNGG 1 cut(s) 171
BseMI GCAATG 1 cut(s) 250
BseMII CTCAG 3 cut(s) 393, 438, 627
BseNI ACTGG 2 cut(s) 409, 648
BseRI GAGGAG 1 cut(s) 682
BseXI GCAGC 4 cut(s) 299, 369, 613, 725
BsgI GTGCAG 2 cut(s) 297, 306
BshFI GGCC 3 cut(s) 317, 408, 567
BshNI GGYRCC 2 cut(s) 61, 229
BshVI ATCGAT 1 cut(s) 114
BsiHKAI GWGCWC 1 cut(s) 752
BsiSI CCGG 3 cut(s) 233, 314, 323
BslFI GGGAC 1 cut(s) 29
BslI CCNNNNNNNGG 1 cut(s) 171
BsmAI GTCTC 3 cut(s) 57, 178, 382
BsmFI GGGAC 1 cut(s) 29
BsnI GGCC 3 cut(s) 317, 408, 567
Bso31I GGTCTC 1 cut(s) 382
Bsp1286I GDGCHC 1 cut(s) 752
Bsp143I GATC 2 cut(s) 34, 111
BspACI CCGC 5 cut(s) 127, 292, 295, 351, 596
BspANI GGCC 3 cut(s) 317, 408, 567
BspCNI CTCAG 3 cut(s) 392, 437, 626
BspDI ATCGAT 1 cut(s) 114
BspHI TCATGA 1 cut(s) 91
BspLI GGNNCC 2 cut(s) 63, 231
BspPI GGATC 1 cut(s) 42
BspQI GCTCTTC 1 cut(s) 740
BspT107I GGYRCC 2 cut(s) 61, 229
BspTNI GGTCTC 1 cut(s) 382
BsrDI GCAATG 1 cut(s) 250
BsrFI RCCGGY 1 cut(s) 313
BsrI ACTGG 2 cut(s) 409, 648
BssAI RCCGGY 1 cut(s) 313
BssECI CCNNGG 3 cut(s) 57, 102, 130
BssMI GATC 2 cut(s) 34, 111
BssT1I CCWWGG 1 cut(s) 102
Bst2UI CCWGG 2 cut(s) 59, 166
Bst6I CTCTTC 4 cut(s) 505, 642, 654, 740
BstAPI GCANNNNNTGC 2 cut(s) 284, 586
BstC8I GCNNGC 6 cut(s) 285, 292, 306, 315, 355, 480
BstDEI CTNAG 5 cut(s) 180, 379, 424, 613, 674
BstDSI CCRYGG 1 cut(s) 130
BstF5I GGATG 1 cut(s) 161
BstHHI GCGC 1 cut(s) 601
BstKTI GATC 2 cut(s) 37, 114
BstMAI GTCTC 3 cut(s) 57, 178, 382
BstMBI GATC 2 cut(s) 34, 111
BstMWI GCNNNNNNNGC 5 cut(s) 284, 310, 314, 586, 734
BstNI CCWGG 2 cut(s) 59, 166
BstNSI RCATGY 1 cut(s) 451
BstSCI CCNGG 3 cut(s) 57, 164, 321
BstV1I GCAGC 4 cut(s) 299, 369, 613, 725
Bsu15I ATCGAT 1 cut(s) 114
BsuRI GGCC 3 cut(s) 317, 408, 567
BsuTUI ATCGAT 1 cut(s) 114
BtgI CCRYGG 1 cut(s) 130
BtsCI GGATG 1 cut(s) 161
BtsI GCAGTG 1 cut(s) 285
BtsIMutI CAGTG 6 cut(s) 107, 285, 387, 402, 525, 728
Cac8I GCNNGC 6 cut(s) 285, 292, 306, 315, 355, 480
CciI TCATGA 1 cut(s) 91
CfoI GCGC 1 cut(s) 601
Cfr10I RCCGGY 1 cut(s) 313
Cfr13I GGNCC 1 cut(s) 316
ClaI ATCGAT 1 cut(s) 114
Csp6I GTAC 4 cut(s) 62, 438, 641, 706
CviAII CATG 7 cut(s) 22, 41, 92, 397, 448, 581, 710
CviQI GTAC 4 cut(s) 62, 438, 641, 706
DdeI CTNAG 5 cut(s) 180, 379, 424, 613, 674
DpnI GATC 2 cut(s) 36, 113
DpnII GATC 2 cut(s) 34, 111
Eam1104I CTCTTC 4 cut(s) 505, 642, 654, 740
EarI CTCTTC 4 cut(s) 505, 642, 654, 740
Ecl136II GAGCTC 1 cut(s) 750
Eco130I CCWWGG 1 cut(s) 102
Eco24I GRGCYC 1 cut(s) 752
Eco31I GGTCTC 1 cut(s) 382
Eco53kI GAGCTC 1 cut(s) 750
Eco57I CTGAAG 1 cut(s) 707
EcoICRI GAGCTC 1 cut(s) 750
EcoRII CCWGG 2 cut(s) 57, 164
EcoT14I CCWWGG 1 cut(s) 102
EcoT38I GRGCYC 1 cut(s) 752
ErhI CCWWGG 1 cut(s) 102
FaeI CATG 7 cut(s) 25, 44, 95, 400, 451, 584, 713
FaqI GGGAC 1 cut(s) 29
FatI CATG 7 cut(s) 21, 40, 91, 396, 447, 580, 709
FauI CCCGC 2 cut(s) 134, 299
FauNDI CATATG 1 cut(s) 367
Fnu4HI GCNGC 7 cut(s) 288, 295, 351, 358, 597, 602, 714
FokI GGATG 1 cut(s) 148
FriOI GRGCYC 1 cut(s) 752
Fsp4HI GCNGC 7 cut(s) 288, 295, 351, 358, 597, 602, 714
GlaI GCGC 1 cut(s) 600
GluI GCNGC 7 cut(s) 288, 295, 351, 358, 597, 602, 714
HaeIII GGCC 3 cut(s) 317, 408, 567
HapII CCGG 3 cut(s) 233, 314, 323
HhaI GCGC 1 cut(s) 601
Hin1II CATG 7 cut(s) 25, 44, 95, 400, 451, 584, 713
Hin6I GCGC 1 cut(s) 599
HinP1I GCGC 1 cut(s) 599
HindIII AAGCTT 1 cut(s) 13
HinfI GANTC 4 cut(s) 329, 530, 542, 670
HpaII CCGG 3 cut(s) 233, 314, 323
HphI GGTGA 1 cut(s) 29
Hpy166II GTNNAC 1 cut(s) 520
Hpy188I TCNGA 4 cut(s) 202, 427, 541, 547
Hpy188III TCNNGA 5 cut(s) 92, 139, 193, 553, 615
Hpy8I GTNNAC 1 cut(s) 520
HpyCH4IV ACGT 1 cut(s) 516
HpyCH4V TGCA 7 cut(s) 220, 243, 278, 287, 385, 580, 713
HpyF10VI GCNNNNNNNGC 5 cut(s) 284, 310, 314, 586, 734
HpyF3I CTNAG 5 cut(s) 180, 379, 424, 613, 674
HpySE526I ACGT 1 cut(s) 516
Hsp92II CATG 7 cut(s) 25, 44, 95, 400, 451, 584, 713
HspAI GCGC 1 cut(s) 599
KpnI GGTACC 1 cut(s) 65
KroI GCCGGC 1 cut(s) 313
KroNI GCCGGC 1 cut(s) 315
Kzo9I GATC 2 cut(s) 34, 111
LguI GCTCTTC 1 cut(s) 740
LmnI GCTCC 2 cut(s) 682, 755
Lsp1109I GCAGC 4 cut(s) 299, 369, 613, 725
LweI GCATC 2 cut(s) 144, 545
MaeII ACGT 1 cut(s) 516
MalI GATC 2 cut(s) 36, 113
MboI GATC 2 cut(s) 34, 111
MboII GAAGA 5 cut(s) 492, 659, 668, 671, 757
MhlI GDGCHC 1 cut(s) 752
MluCI AATT 4 cut(s) 142, 147, 334, 485
MlyI GAGTC 1 cut(s) 679
MnlI CCTC 5 cut(s) 329, 367, 419, 660, 750
MroNI GCCGGC 1 cut(s) 313
MseI TTAA 2 cut(s) 146, 570
MspA1I CMGCKG 1 cut(s) 596
MspI CCGG 3 cut(s) 233, 314, 323
MspR9I CCNGG 3 cut(s) 59, 166, 323
MvaI CCWGG 2 cut(s) 59, 166
MwoI GCNNNNNNNGC 5 cut(s) 284, 310, 314, 586, 734
NaeI GCCGGC 1 cut(s) 315
NciI CCSGG 1 cut(s) 323
NdeI CATATG 1 cut(s) 367
NdeII GATC 2 cut(s) 34, 111
NgoMIV GCCGGC 1 cut(s) 313
NlaIII CATG 7 cut(s) 25, 44, 95, 400, 451, 584, 713
NlaIV GGNNCC 2 cut(s) 63, 231
NmeAIII GCCGAG 1 cut(s) 323
NspI RCATGY 1 cut(s) 451
PagI TCATGA 1 cut(s) 91
PciSI GCTCTTC 1 cut(s) 740
PcsI WCGNNNNNNNCGW 1 cut(s) 558
PdiI GCCGGC 1 cut(s) 315
PfeI GAWTC 3 cut(s) 329, 530, 542
PflFI GACNNNGTC 1 cut(s) 374
PfoI TCCNGGA 1 cut(s) 164
PkrI GCNGC 7 cut(s) 289, 296, 352, 359, 598, 603, 715
PleI GAGTC 1 cut(s) 678
PpsI GAGTC 1 cut(s) 678
Psp124BI GAGCTC 1 cut(s) 752
Psp6I CCWGG 2 cut(s) 57, 164
PspGI CCWGG 2 cut(s) 57, 164
PspN4I GGNNCC 2 cut(s) 63, 231
PspPI GGNCC 1 cut(s) 316
PsyI GACNNNGTC 1 cut(s) 374
RsaI GTAC 4 cut(s) 63, 439, 642, 707
RsaNI GTAC 4 cut(s) 62, 438, 641, 706
SacI GAGCTC 1 cut(s) 752
SapI GCTCTTC 1 cut(s) 740
SaqAI TTAA 2 cut(s) 146, 570
SatI GCNGC 7 cut(s) 288, 295, 351, 358, 597, 602, 714
Sau3AI GATC 2 cut(s) 34, 111
Sau96I GGNCC 1 cut(s) 316
ScaI AGTACT 2 cut(s) 439, 642
SchI GAGTC 1 cut(s) 679
ScrFI CCNGG 3 cut(s) 59, 166, 323
SduI GDGCHC 1 cut(s) 752
SetI ASST 7 cut(s) 17, 90, 378, 484, 519, 687, 752
SfaNI GCATC 2 cut(s) 144, 545
SmlI CTYRAG 2 cut(s) 29, 83
SmoI CTYRAG 2 cut(s) 29, 83
Sse9I AATT 4 cut(s) 142, 147, 334, 485
SsiI CCGC 5 cut(s) 127, 292, 295, 351, 596
SstI GAGCTC 1 cut(s) 752
StyD4I CCNGG 3 cut(s) 57, 164, 321
StyI CCWWGG 1 cut(s) 102
TaiI ACGT 1 cut(s) 519
TaqI TCGA 2 cut(s) 114, 561
TasI AATT 4 cut(s) 142, 147, 334, 485
TatI WGTACW 2 cut(s) 437, 640
TauI GCSGC 3 cut(s) 297, 353, 599
TfiI GAWTC 3 cut(s) 329, 530, 542
Tru1I TTAA 2 cut(s) 146, 570
Tru9I TTAA 2 cut(s) 146, 570
TscAI CASTG 6 cut(s) 114, 285, 387, 409, 532, 735
TseI GCWGC 4 cut(s) 287, 357, 601, 713
TspDTI ATGAA 4 cut(s) 80, 150, 328, 597
TspRI CASTG 6 cut(s) 114, 285, 387, 409, 532, 735
Tth111I GACNNNGTC 1 cut(s) 374
XapI RAATTY 1 cut(s) 142
XceI RCATGY 1 cut(s) 451
ZrmI AGTACT 2 cut(s) 439, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.