RLG00000009422

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0014277
Physical Location & Seq
Reverse (-)
52634888 .. 52637701
2814 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009422

Sequence Viewer

Length: 1428 bp
ATGTTTTCCGTAGACTCATTTAACTTGAAGGAAGGAAGAGGAGGGAGGGGGGAGAGAGTGATGTGGAATTCGAATAGCAGAAATAGTGGAAAAAGAAGACGATACAATGGGGGGCCAATAGCAGTAGGAGTATCAGGAGACAAAGCCAGCCAAAATGCTTTGAGATGGGCCATTGATAATCTTGTCACTCCTCCGAGCGACGATGTTGTTGTCAAGCTTCTCCATGTCGCTCAAGGATCACCATGTCCCATTGTCTCCCCTGTTGGGCATTGTGGGAATTTAGTGGGAAAGCCAAACTATTTACTGGTGAGCGTTGGATTGATTAATGAAGGGCAAGCTTTTGATCCTATTGAGGAAATTTTACTTCCCTTTCGTTGCTATTGTACACGCAAACGAGTACCATTTGAGATTGTTATACTCAAGGATCATGATGTAGCCAAGGCACTGATCGATTACATTTCCCGCCGTGGCATCGGGAATTTAGTTCTTGGTGCTTCATCCAGGAATGGGCTTTCTAAGAGGTACAGCTACCATTACCAATCAATTTCATCTTTTCCATTTAGTGCTTTATTACATTATATTTATATTCCAAGTAGTGTGCAAAAGTTGGCACCGGATTTCTGCAATGTACTTACTATCAGCAAAGGAAAGATACGTGCAGTGCGTGCAGCCCGCCGCCGAGTGCCTGCCCTGCCGGCCCTCCCGGCAGAATCTAATTACATAGCCGCAAGCAGCAAATCATATGACGAGGTCTCAGTGCAAGAACCTGACATGGACACTGGTCTCTCATATGTAGGCTCAGAAAGACGAAGTACTGATAGAATGTTGTATTCTTTCTACGAAAACTTTGGGCAAGCTGAATTGAGCCAAATCTCTTCTATGTCCTTGAACGTGGACATCACTGCTTCAGCATCAGAATCAGATTTCGGGAGTTTTGATGGCCTTAACACTATGCATGAAATGCCATCAGCAGCGCAGCACAATGGCTCAGGAAATCCATTCTTGTCGCAGAAAGTACTGCAAGAGATGGAAGAAGAGATGAAAAGTCTTAGAATGGAGCTGAAGCAAACGATAGAGATGTACCATTCAGCCTATAAAGAAGCACTGTCAGCAAAGCAGAAGGCTACAGAGCTCCAAGAGGGGAAAATGAAAGAAGAACAGAGACTAGAGGAAGCACTACTCACCTTAGAAGCAACTACAGCAGCTTTGAAGAACGAAAAAGAAAAGTCTAAGGCAGCAATATTGGCAGCAGAAGCAGCTCAGAGGCTTGTTGAGGAAGAGGTGCAAAAGAAAGCGAATGTTGACAAGATGGAGGAGAACAAGGTTTTGGATACTTTTGGCCAGTTCTTTTTAGCTCTCAAATACCAAAGCTTATTTCATATCGTAGTTGCTTTGATTTTATTCTATGTATACTTCTCAATCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

476

Amino Acids

52.86

Weight (kDa)

7.63

Isoelectric Point (pI)

57.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014277)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20310
malus_domestica MD13G1232700.v1.1 MD13G1233300.v1.1 MD16G1238300.v1.1
prunus_persica Prupe.1G064200_v2.0.a1
pyrus_communis pycom13g20550
rosa_chinensis RchiOBHm_Chr4g0397631
rosa_laevigata RLG00000009422
rosa_multiflora Rmu_co8218642.1_g000001 Rmu_sc0002729.1_g000005
rosa_roxburghii Rroxscaffold_5G00342440
rosa_rugosa Rorug03G0342200
rosa_samantha Rh4AG079500 Rh4BG077500 Rh4CG085400 Rh4DG072500
rosa_wichuraiana Rw4G006510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 610
AccI GTMKAC 2 cut(s) 12, 1410
AciI CCGC 4 cut(s) 463, 673, 676, 726
AclWI GGATC 3 cut(s) 244, 338, 432
AcoI YGGCCR 1 cut(s) 1339
AcsI RAATTY 4 cut(s) 67, 277, 357, 478
AcuI CTGAAG 2 cut(s) 891, 1082
AfaI GTAC 7 cut(s) 385, 399, 524, 630, 814, 1017, 1082
AfiI CCNNNNNNNGG 2 cut(s) 264, 507
AgsI TTSAA 3 cut(s) 28, 889, 1210
AjnI CCWGG 1 cut(s) 500
AjuI GAANNNNNNNTTGG 4 cut(s) 1310, 1342, 1359, 1391
Alw21I GWGCWC 1 cut(s) 1134
Alw26I GTCTC 5 cut(s) 132, 259, 757, 788, 1156
AlwI GGATC 3 cut(s) 244, 338, 432
AoxI GGCC 5 cut(s) 113, 168, 696, 940, 1339
ApeKI GCWGC 8 cut(s) 668, 732, 971, 976, 1202, 1235, 1247, 1256
ApoI RAATTY 4 cut(s) 67, 277, 357, 478
AseI ATTAAT 1 cut(s) 324
AspLEI GCGC 1 cut(s) 976
AspS9I GGNCC 3 cut(s) 113, 168, 697
AsuC2I CCSGG 1 cut(s) 704
AsuHPI GGTGA 3 cut(s) 231, 319, 1174
AsuII TTCGAA 1 cut(s) 71
BalI TGGCCA 1 cut(s) 1341
BanI GGYRCC 1 cut(s) 610
BanII GRGCYC 1 cut(s) 1134
BbsI GAAGAC 1 cut(s) 103
Bbv12I GWGCWC 1 cut(s) 1134
BbvI GCAGC 8 cut(s) 680, 744, 983, 988, 1214, 1247, 1259, 1268
BccI CCATC 5 cut(s) 159, 932, 973, 1021, 1303
BceAI ACGGC 1 cut(s) 450
BciT130I CCWGG 1 cut(s) 502
BciVI GTATCC 1 cut(s) 1324
BcnI CCSGG 1 cut(s) 704
BcoDI GTCTC 5 cut(s) 132, 259, 757, 788, 1156
BfaI CTAG 1 cut(s) 1166
BfmI CTRYAG 2 cut(s) 1125, 1197
BfuI GTATCC 1 cut(s) 1324
BglI GCCNNNNNGGC 2 cut(s) 695, 704
BmcAI AGTACT 2 cut(s) 814, 1017
Bme1390I CCNGG 2 cut(s) 502, 704
BmgT120I GGNCC 3 cut(s) 113, 168, 697
BmiI GGNNCC 2 cut(s) 114, 612
BmrFI CCNGG 2 cut(s) 502, 704
BmsI GCATC 2 cut(s) 480, 920
BoxI GACNNNNGTC 1 cut(s) 780
BpiI GAAGAC 1 cut(s) 103
Bpu10I CCTNAGC 1 cut(s) 988
Bpu14I TTCGAA 1 cut(s) 71
BpuEI CTTGAG 2 cut(s) 216, 404
BpuMI CCSGG 1 cut(s) 704
Bsa29I ATCGAT 1 cut(s) 450
BsaAI YACGTR 1 cut(s) 656
BsaI GGTCTC 2 cut(s) 757, 788
BsaJI CCNNGG 2 cut(s) 438, 466
BsaWI WCCGGW 1 cut(s) 613
Bsc4I CCNNNNNNNGG 2 cut(s) 264, 507
Bse118I RCCGGY 1 cut(s) 694
Bse1I ACTGG 3 cut(s) 309, 784, 1342
Bse3DI GCAATG 1 cut(s) 631
BseBI CCWGG 1 cut(s) 502
BseCI ATCGAT 1 cut(s) 450
BseDI CCNNGG 2 cut(s) 438, 466
BseGI GGATG 1 cut(s) 497
BseLI CCNNNNNNNGG 2 cut(s) 264, 507
BseMI GCAATG 1 cut(s) 631
BseMII CTCAG 4 cut(s) 768, 813, 1002, 1274
BseNI ACTGG 3 cut(s) 309, 784, 1342
BseRI GAGGAG 3 cut(s) 54, 180, 1328
BseXI GCAGC 8 cut(s) 680, 744, 983, 988, 1214, 1247, 1259, 1268
BsgI GTGCAG 2 cut(s) 678, 687
BshFI GGCC 5 cut(s) 115, 170, 698, 942, 1341
BshNI GGYRCC 1 cut(s) 610
BshVI ATCGAT 1 cut(s) 450
BsiHKAI GWGCWC 1 cut(s) 1134
BsiSI CCGG 3 cut(s) 614, 695, 704
BslFI GGGAC 1 cut(s) 231
BslI CCNNNNNNNGG 2 cut(s) 264, 507
BsmAI GTCTC 5 cut(s) 132, 259, 757, 788, 1156
BsmFI GGGAC 1 cut(s) 231
BsnI GGCC 5 cut(s) 115, 170, 698, 942, 1341
Bso31I GGTCTC 2 cut(s) 757, 788
Bsp119I TTCGAA 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 1134
Bsp1407I TGTACA 1 cut(s) 383
Bsp143I GATC 4 cut(s) 236, 343, 424, 447
BspACI CCGC 4 cut(s) 463, 673, 676, 726
BspANI GGCC 5 cut(s) 115, 170, 698, 942, 1341
BspCNI CTCAG 4 cut(s) 767, 812, 1001, 1273
BspDI ATCGAT 1 cut(s) 450
BspHI TCATGA 1 cut(s) 427
BspLI GGNNCC 2 cut(s) 114, 612
BspPI GGATC 3 cut(s) 244, 338, 432
BspT104I TTCGAA 1 cut(s) 71
BspT107I GGYRCC 1 cut(s) 610
BspTNI GGTCTC 2 cut(s) 757, 788
BsrDI GCAATG 1 cut(s) 631
BsrFI RCCGGY 1 cut(s) 694
BsrGI TGTACA 1 cut(s) 383
BsrI ACTGG 3 cut(s) 309, 784, 1342
BssAI RCCGGY 1 cut(s) 694
BssECI CCNNGG 2 cut(s) 438, 466
BssMI GATC 4 cut(s) 236, 343, 424, 447
BssNAI GTATAC 1 cut(s) 1411
BssT1I CCWWGG 1 cut(s) 438
Bst1107I GTATAC 1 cut(s) 1411
Bst2UI CCWGG 1 cut(s) 502
Bst4CI ACNGT 1 cut(s) 1107
Bst6I CTCTTC 4 cut(s) 31, 880, 1029, 1272
BstAPI GCANNNNNTGC 2 cut(s) 665, 961
BstAUI TGTACA 1 cut(s) 383
BstBAI YACGTR 1 cut(s) 656
BstBI TTCGAA 1 cut(s) 71
BstC8I GCNNGC 8 cut(s) 148, 336, 666, 673, 687, 696, 730, 855
BstDEI CTNAG 8 cut(s) 516, 754, 799, 988, 1049, 1186, 1230, 1260
BstDSI CCRYGG 1 cut(s) 466
BstF5I GGATG 1 cut(s) 497
BstHHI GCGC 1 cut(s) 976
BstKTI GATC 4 cut(s) 239, 346, 427, 450
BstMAI GTCTC 5 cut(s) 132, 259, 757, 788, 1156
BstMBI GATC 4 cut(s) 236, 343, 424, 447
BstNI CCWGG 1 cut(s) 502
BstPAI GACNNNNGTC 1 cut(s) 780
BstSCI CCNGG 2 cut(s) 500, 702
BstSFI CTRYAG 2 cut(s) 1125, 1197
BstV1I GCAGC 8 cut(s) 680, 744, 983, 988, 1214, 1247, 1259, 1268
BstV2I GAAGAC 1 cut(s) 103
BstZ17I GTATAC 1 cut(s) 1411
Bsu15I ATCGAT 1 cut(s) 450
BsuI GTATCC 1 cut(s) 1324
BsuRI GGCC 5 cut(s) 115, 170, 698, 942, 1341
BsuTUI ATCGAT 1 cut(s) 450
BtgI CCRYGG 1 cut(s) 466
BtsCI GGATG 1 cut(s) 497
BtsI GCAGTG 2 cut(s) 666, 900
BtsIMutI CAGTG 6 cut(s) 443, 666, 762, 777, 900, 1103
Cac8I GCNNGC 8 cut(s) 148, 336, 666, 673, 687, 696, 730, 855
CciI TCATGA 1 cut(s) 427
CfoI GCGC 1 cut(s) 976
Cfr10I RCCGGY 1 cut(s) 694
Cfr13I GGNCC 3 cut(s) 113, 168, 697
ClaI ATCGAT 1 cut(s) 450
Csp6I GTAC 7 cut(s) 384, 398, 523, 629, 813, 1016, 1081
CviAII CATG 5 cut(s) 224, 243, 428, 772, 956
CviQI GTAC 7 cut(s) 384, 398, 523, 629, 813, 1016, 1081
DdeI CTNAG 8 cut(s) 516, 754, 799, 988, 1049, 1186, 1230, 1260
DpnI GATC 4 cut(s) 238, 345, 426, 449
DpnII GATC 4 cut(s) 236, 343, 424, 447
EaeI YGGCCR 1 cut(s) 1339
Eam1104I CTCTTC 4 cut(s) 31, 880, 1029, 1272
EarI CTCTTC 4 cut(s) 31, 880, 1029, 1272
Ecl136II GAGCTC 1 cut(s) 1132
Eco130I CCWWGG 1 cut(s) 438
Eco24I GRGCYC 1 cut(s) 1134
Eco31I GGTCTC 2 cut(s) 757, 788
Eco53kI GAGCTC 1 cut(s) 1132
Eco57I CTGAAG 2 cut(s) 891, 1082
EcoICRI GAGCTC 1 cut(s) 1132
EcoRI GAATTC 1 cut(s) 67
EcoRII CCWGG 1 cut(s) 500
EcoT14I CCWWGG 1 cut(s) 438
EcoT22I ATGCAT 1 cut(s) 957
EcoT38I GRGCYC 1 cut(s) 1134
ErhI CCWWGG 1 cut(s) 438
FaeI CATG 5 cut(s) 227, 246, 431, 775, 959
FaqI GGGAC 1 cut(s) 231
FatI CATG 5 cut(s) 223, 242, 427, 771, 955
FauI CCCGC 2 cut(s) 470, 680
FauNDI CATATG 2 cut(s) 742, 790
FblI GTMKAC 2 cut(s) 12, 1410
FokI GGATG 1 cut(s) 484
FriOI GRGCYC 1 cut(s) 1134
FspBI CTAG 1 cut(s) 1166
GlaI GCGC 1 cut(s) 975
HaeIII GGCC 5 cut(s) 115, 170, 698, 942, 1341
HapII CCGG 3 cut(s) 614, 695, 704
HhaI GCGC 1 cut(s) 976
Hin1II CATG 5 cut(s) 227, 246, 431, 775, 959
Hin6I GCGC 1 cut(s) 974
HinP1I GCGC 1 cut(s) 974
HincII GTYRAC 1 cut(s) 1303
HindII GTYRAC 1 cut(s) 1303
HindIII AAGCTT 3 cut(s) 215, 336, 1369
HinfI GANTC 3 cut(s) 14, 710, 917
HpaII CCGG 3 cut(s) 614, 695, 704
HphI GGTGA 3 cut(s) 231, 319, 1174
Hpy166II GTNNAC 5 cut(s) 13, 386, 895, 1303, 1411
Hpy188I TCNGA 5 cut(s) 195, 802, 916, 922, 1263
Hpy188III TCNNGA 5 cut(s) 135, 428, 475, 928, 990
Hpy8I GTNNAC 5 cut(s) 13, 386, 895, 1303, 1411
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 4 cut(s) 22, 26, 323, 1114
HpyCH4III ACNGT 1 cut(s) 1107
HpyCH4IV ACGT 2 cut(s) 655, 891
HpyCH4V TGCA 8 cut(s) 601, 624, 659, 668, 760, 955, 1021, 1285
HpyF3I CTNAG 8 cut(s) 516, 754, 799, 988, 1049, 1186, 1230, 1260
HpySE526I ACGT 2 cut(s) 655, 891
Hsp92II CATG 5 cut(s) 227, 246, 431, 775, 959
HspAI GCGC 1 cut(s) 974
KroI GCCGGC 1 cut(s) 694
KroNI GCCGGC 1 cut(s) 696
Kzo9I GATC 4 cut(s) 236, 343, 424, 447
LmnI GCTCC 2 cut(s) 1057, 1137
Lsp1109I GCAGC 8 cut(s) 680, 744, 983, 988, 1214, 1247, 1259, 1268
LweI GCATC 2 cut(s) 480, 920
MaeI CTAG 1 cut(s) 1166
MaeII ACGT 2 cut(s) 655, 891
MaeIII GTNAC 1 cut(s) 184
MalI GATC 4 cut(s) 238, 345, 426, 449
MboI GATC 4 cut(s) 236, 343, 424, 447
MboII GAAGA 8 cut(s) 48, 108, 867, 1043, 1046, 1166, 1222, 1289
MhlI GDGCHC 1 cut(s) 1134
MlsI TGGCCA 1 cut(s) 1341
MluCI AATT 7 cut(s) 67, 277, 357, 478, 543, 715, 860
MluNI TGGCCA 1 cut(s) 1341
MlyI GAGTC 1 cut(s) 8
MmeI TCCRAC 1 cut(s) 295
Mox20I TGGCCA 1 cut(s) 1341
Mph1103I ATGCAT 1 cut(s) 957
MroNI GCCGGC 1 cut(s) 694
MscI TGGCCA 1 cut(s) 1341
MseI TTAA 3 cut(s) 21, 324, 945
Msp20I TGGCCA 1 cut(s) 1341
MspI CCGG 3 cut(s) 614, 695, 704
MspR9I CCNGG 2 cut(s) 502, 704
MvaI CCWGG 1 cut(s) 502
NaeI GCCGGC 1 cut(s) 696
NciI CCSGG 1 cut(s) 704
NdeI CATATG 2 cut(s) 742, 790
NdeII GATC 4 cut(s) 236, 343, 424, 447
NgoMIV GCCGGC 1 cut(s) 694
NlaIII CATG 5 cut(s) 227, 246, 431, 775, 959
NlaIV GGNNCC 2 cut(s) 114, 612
NmeAIII GCCGAG 1 cut(s) 704
NmuCI GTSAC 1 cut(s) 184
NsiI ATGCAT 1 cut(s) 957
NspV TTCGAA 1 cut(s) 71
PagI TCATGA 1 cut(s) 427
PcsI WCGNNNNNNNCGW 1 cut(s) 661
PdiI GCCGGC 1 cut(s) 696
PfeI GAWTC 2 cut(s) 710, 917
PflFI GACNNNGTC 1 cut(s) 749
PfoI TCCNGGA 1 cut(s) 500
PleI GAGTC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 8
Ppu21I YACGTR 1 cut(s) 656
PshAI GACNNNNGTC 1 cut(s) 780
PshBI ATTAAT 1 cut(s) 324
Psp124BI GAGCTC 1 cut(s) 1134
Psp6I CCWGG 1 cut(s) 500
PspGI CCWGG 1 cut(s) 500
PspN4I GGNNCC 2 cut(s) 114, 612
PspPI GGNCC 3 cut(s) 113, 168, 697
PsyI GACNNNGTC 1 cut(s) 749
RsaI GTAC 7 cut(s) 385, 399, 524, 630, 814, 1017, 1082
RsaNI GTAC 7 cut(s) 384, 398, 523, 629, 813, 1016, 1081
SacI GAGCTC 1 cut(s) 1134
SaqAI TTAA 3 cut(s) 21, 324, 945
Sau3AI GATC 4 cut(s) 236, 343, 424, 447
Sau96I GGNCC 3 cut(s) 113, 168, 697
ScaI AGTACT 2 cut(s) 814, 1017
SchI GAGTC 1 cut(s) 8
ScrFI CCNGG 2 cut(s) 502, 704
SduI GDGCHC 1 cut(s) 1134
SfaNI GCATC 2 cut(s) 480, 920
SfcI CTRYAG 2 cut(s) 1125, 1197
SfuI TTCGAA 1 cut(s) 71
SmlI CTYRAG 2 cut(s) 231, 419
SmoI CTYRAG 2 cut(s) 231, 419
Sse9I AATT 7 cut(s) 67, 277, 357, 478, 543, 715, 860
SsiI CCGC 4 cut(s) 463, 673, 676, 726
SspI AATATT 1 cut(s) 1242
SspMI CTAG 1 cut(s) 1166
SstI GAGCTC 1 cut(s) 1134
StyD4I CCNGG 2 cut(s) 500, 702
StyI CCWWGG 1 cut(s) 438
TaaI ACNGT 1 cut(s) 1107
TaiI ACGT 2 cut(s) 658, 894
TaqI TCGA 2 cut(s) 71, 450
TasI AATT 7 cut(s) 67, 277, 357, 478, 543, 715, 860
TatI WGTACW 4 cut(s) 383, 628, 812, 1015
TauI GCSGC 2 cut(s) 678, 728
TfiI GAWTC 2 cut(s) 710, 917
Tru1I TTAA 3 cut(s) 21, 324, 945
Tru9I TTAA 3 cut(s) 21, 324, 945
TscAI CASTG 6 cut(s) 450, 666, 762, 784, 907, 1110
TseFI GTSAC 1 cut(s) 184
TseI GCWGC 8 cut(s) 668, 732, 971, 976, 1202, 1235, 1247, 1256
Tsp45I GTSAC 1 cut(s) 184
TspDTI ATGAA 7 cut(s) 342, 486, 537, 972, 1055, 1163, 1367
TspRI CASTG 6 cut(s) 450, 666, 762, 784, 907, 1110
Tth111I GACNNNGTC 1 cut(s) 749
VspI ATTAAT 1 cut(s) 324
XapI RAATTY 4 cut(s) 67, 277, 357, 478
XmiI GTMKAC 2 cut(s) 12, 1410
XspI CTAG 1 cut(s) 1166
ZrmI AGTACT 2 cut(s) 814, 1017
Zsp2I ATGCAT 1 cut(s) 957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.