Rh4CG085400

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0014277
Physical Location & Seq
Forward (+)
15572798 .. 15575120
2323 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG085400.1

Sequence Viewer

Length: 1032 bp
ATGTCGCTCAAGGATCACCATGTCCCATTGTCTCCCCTGGTACCATATGAGATTGTTATACTCAAGGATCATGATGTAGCCAAGGCACTGATCGATTACATTTCCCGCCGTGGCATCGGGAATTTAATTCTTGGTGCTTCATCCAGGAATGGGTTTTCTAAGAGACTATTCAAGACATCAGATATTCCAAGTAGTGTGCAAAAGTTGGCACCGGATTTCTGCAATGTATTTACTATCAGCAAAGGAAAGATATGTGAAGTGCGTGCAGCCTGCCGCCGAGTGCCTGCCCTGCCGACCCTCCCGGAAGAATCTAATTACATAGGCAGAGCCGCAAGCAGCAAATCATATGACGAGGTCTCAGTGCAAGAGCCTGACATGGACACTGGCCTCTCTTTTGTAGGCTCAGAAAGACAAAGTACTGATAGCATGTTGTATTCTTTCTACGAAAACTTTGGGCAAGCTGAATTGACCCAAATCTCTTCTATGTCCTTGATCGTGGACATCACTGAATCAGCATCAGAATCAGATTTCAGGAGTTTCGATGGCCTTAACACTTTGCATGAAATGCCATCAGCGGAGCAGCACAATGGCTCAGGAAATCCATTCTTGTCGCAGAAAGTACTGGAAGAGATGGAAGAAGAGATGAAAAGTCTTAGAATGGAGCTGAAGCAAACGATAGAGATGTACCATGCAGCCTATAAAGAAGCACTAACAGCAAAGCAGAAGGCTACAGAGCTCCAAGAGGGGAAAATGAAAGAAGAACAGAGACTAGAGGAAGCACTACTCACCTTAGAAGCAACTACAGCAGCTTTGAAGAACGAAAAAGAAAAGTCTAAGGCAGCGATATTGGCAGCAGAAGCAGCTCAGAGGCTTGTTGAGGAAGAGGTGCAAAAGAGAGTGAATGCTGACAAGATGGAGGAGAACAAGGTTTTGGATACTTTTGGCCAGTTCTTTTTAGCTCTCAAGTACCAAAGCTTATTTCATATCGTAGTTGCTTTGATTTTATTCTATGTGTACTTCTCAATCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

343

Amino Acids

38.54

Weight (kDa)

5.23

Isoelectric Point (pI)

56.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014277)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20310
malus_domestica MD13G1232700.v1.1 MD13G1233300.v1.1 MD16G1238300.v1.1
prunus_persica Prupe.1G064200_v2.0.a1
pyrus_communis pycom13g20550
rosa_chinensis RchiOBHm_Chr4g0397631
rosa_laevigata RLG00000009422
rosa_multiflora Rmu_co8218642.1_g000001 Rmu_sc0002729.1_g000005
rosa_roxburghii Rroxscaffold_5G00342440
rosa_rugosa Rorug03G0342200
rosa_samantha Rh4AG079500 Rh4BG077500 Rh4CG085400 Rh4DG072500
rosa_wichuraiana Rw4G006510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 40
AccB1I GGYRCC 2 cut(s) 40, 208
AciI CCGC 4 cut(s) 106, 274, 330, 575
AclWI GGATC 2 cut(s) 21, 75
AcoI YGGCCR 1 cut(s) 943
AcsI RAATTY 1 cut(s) 121
AcuI CTGAAG 1 cut(s) 686
AfaI GTAC 6 cut(s) 42, 418, 621, 686, 968, 1016
AfiI CCNNNNNNNGG 1 cut(s) 150
AgsI TTSAA 2 cut(s) 172, 814
AjnI CCWGG 2 cut(s) 36, 143
AjuI GAANNNNNNNTTGG 4 cut(s) 914, 946, 963, 995
AluBI AGCT 7 cut(s) 461, 664, 736, 809, 863, 959, 975
AluI AGCT 7 cut(s) 461, 664, 736, 809, 863, 959, 975
Alw21I GWGCWC 1 cut(s) 738
Alw26I GTCTC 4 cut(s) 36, 157, 361, 760
AlwI GGATC 2 cut(s) 21, 75
AoxI GGCC 3 cut(s) 385, 544, 943
ApeKI GCWGC 8 cut(s) 266, 336, 580, 692, 806, 839, 851, 860
ApoI RAATTY 1 cut(s) 121
Asp718I GGTACC 1 cut(s) 40
AsuC2I CCSGG 1 cut(s) 302
AsuHPI GGTGA 2 cut(s) 8, 778
BalI TGGCCA 1 cut(s) 945
BanI GGYRCC 2 cut(s) 40, 208
BanII GRGCYC 1 cut(s) 738
Bbv12I GWGCWC 1 cut(s) 738
BbvI GCAGC 8 cut(s) 278, 348, 592, 704, 818, 851, 863, 872
BccI CCATC 4 cut(s) 536, 577, 625, 907
BceAI ACGGC 1 cut(s) 93
BciT130I CCWGG 2 cut(s) 38, 145
BciVI GTATCC 1 cut(s) 928
BcnI CCSGG 1 cut(s) 302
BcoDI GTCTC 4 cut(s) 36, 157, 361, 760
BfaI CTAG 1 cut(s) 770
BfmI CTRYAG 2 cut(s) 729, 801
BfuI GTATCC 1 cut(s) 928
BmcAI AGTACT 2 cut(s) 418, 621
Bme1390I CCNGG 3 cut(s) 38, 145, 302
BmiI GGNNCC 2 cut(s) 42, 210
BmrFI CCNGG 3 cut(s) 38, 145, 302
BmsI GCATC 2 cut(s) 123, 524
Bpu10I CCTNAGC 1 cut(s) 592
BpuEI CTTGAG 2 cut(s) 47, 947
BpuMI CCSGG 1 cut(s) 302
Bsa29I ATCGAT 1 cut(s) 93
BsaI GGTCTC 1 cut(s) 361
BsaJI CCNNGG 3 cut(s) 36, 81, 109
BsaWI WCCGGW 1 cut(s) 211
Bsc4I CCNNNNNNNGG 1 cut(s) 150
Bse1I ACTGG 3 cut(s) 388, 627, 946
Bse3DI GCAATG 1 cut(s) 229
BseBI CCWGG 2 cut(s) 38, 145
BseCI ATCGAT 1 cut(s) 93
BseDI CCNNGG 3 cut(s) 36, 81, 109
BseGI GGATG 1 cut(s) 140
BseLI CCNNNNNNNGG 1 cut(s) 150
BseMI GCAATG 1 cut(s) 229
BseMII CTCAG 4 cut(s) 372, 417, 606, 878
BseNI ACTGG 3 cut(s) 388, 627, 946
BseRI GAGGAG 1 cut(s) 932
BseXI GCAGC 8 cut(s) 278, 348, 592, 704, 818, 851, 863, 872
BsgI GTGCAG 1 cut(s) 285
BshFI GGCC 3 cut(s) 387, 546, 945
BshNI GGYRCC 2 cut(s) 40, 208
BshVI ATCGAT 1 cut(s) 93
BsiHKAI GWGCWC 1 cut(s) 738
BsiSI CCGG 2 cut(s) 212, 302
BslFI GGGAC 1 cut(s) 8
BslI CCNNNNNNNGG 1 cut(s) 150
BsmAI GTCTC 4 cut(s) 36, 157, 361, 760
BsmFI GGGAC 1 cut(s) 8
BsmI GAATGC 1 cut(s) 907
BsnI GGCC 3 cut(s) 387, 546, 945
Bso31I GGTCTC 1 cut(s) 361
Bsp1286I GDGCHC 1 cut(s) 738
Bsp143I GATC 4 cut(s) 13, 67, 90, 492
BspACI CCGC 4 cut(s) 106, 274, 330, 575
BspANI GGCC 3 cut(s) 387, 546, 945
BspCNI CTCAG 4 cut(s) 371, 416, 605, 877
BspDI ATCGAT 1 cut(s) 93
BspHI TCATGA 1 cut(s) 70
BspLI GGNNCC 2 cut(s) 42, 210
BspPI GGATC 2 cut(s) 21, 75
BspT107I GGYRCC 2 cut(s) 40, 208
BspTNI GGTCTC 1 cut(s) 361
BsrDI GCAATG 1 cut(s) 229
BsrI ACTGG 3 cut(s) 388, 627, 946
BssECI CCNNGG 3 cut(s) 36, 81, 109
BssMI GATC 4 cut(s) 13, 67, 90, 492
BssT1I CCWWGG 1 cut(s) 81
Bst2UI CCWGG 2 cut(s) 38, 145
Bst6I CTCTTC 4 cut(s) 484, 621, 633, 876
BstAPI GCANNNNNTGC 1 cut(s) 565
BstC8I GCNNGC 5 cut(s) 264, 271, 285, 334, 459
BstDEI CTNAG 8 cut(s) 159, 358, 403, 592, 653, 790, 834, 864
BstDSI CCRYGG 1 cut(s) 109
BstF5I GGATG 1 cut(s) 140
BstKTI GATC 4 cut(s) 16, 70, 93, 495
BstMAI GTCTC 4 cut(s) 36, 157, 361, 760
BstMBI GATC 4 cut(s) 13, 67, 90, 492
BstMWI GCNNNNNNNGC 7 cut(s) 289, 565, 713, 803, 848, 857, 860
BstNI CCWGG 2 cut(s) 38, 145
BstNSI RCATGY 1 cut(s) 430
BstSCI CCNGG 3 cut(s) 36, 143, 300
BstSFI CTRYAG 2 cut(s) 729, 801
BstV1I GCAGC 8 cut(s) 278, 348, 592, 704, 818, 851, 863, 872
Bsu15I ATCGAT 1 cut(s) 93
BsuI GTATCC 1 cut(s) 928
BsuRI GGCC 3 cut(s) 387, 546, 945
BsuTUI ATCGAT 1 cut(s) 93
BtgI CCRYGG 1 cut(s) 109
BtsCI GGATG 1 cut(s) 140
BtsIMutI CAGTG 4 cut(s) 86, 366, 381, 504
Cac8I GCNNGC 5 cut(s) 264, 271, 285, 334, 459
CciI TCATGA 1 cut(s) 70
ClaI ATCGAT 1 cut(s) 93
Csp6I GTAC 6 cut(s) 41, 417, 620, 685, 967, 1015
CviAII CATG 6 cut(s) 20, 71, 376, 427, 560, 689
CviQI GTAC 6 cut(s) 41, 417, 620, 685, 967, 1015
DdeI CTNAG 8 cut(s) 159, 358, 403, 592, 653, 790, 834, 864
DpnI GATC 4 cut(s) 15, 69, 92, 494
DpnII GATC 4 cut(s) 13, 67, 90, 492
EaeI YGGCCR 1 cut(s) 943
Eam1104I CTCTTC 4 cut(s) 484, 621, 633, 876
EarI CTCTTC 4 cut(s) 484, 621, 633, 876
Ecl136II GAGCTC 1 cut(s) 736
Eco130I CCWWGG 1 cut(s) 81
Eco24I GRGCYC 1 cut(s) 738
Eco31I GGTCTC 1 cut(s) 361
Eco53kI GAGCTC 1 cut(s) 736
Eco57I CTGAAG 1 cut(s) 686
EcoICRI GAGCTC 1 cut(s) 736
EcoRII CCWGG 2 cut(s) 36, 143
EcoT14I CCWWGG 1 cut(s) 81
EcoT38I GRGCYC 1 cut(s) 738
ErhI CCWWGG 1 cut(s) 81
FaeI CATG 6 cut(s) 23, 74, 379, 430, 563, 692
FaqI GGGAC 1 cut(s) 8
FatI CATG 6 cut(s) 19, 70, 375, 426, 559, 688
FauI CCCGC 1 cut(s) 113
FauNDI CATATG 2 cut(s) 46, 346
FokI GGATG 1 cut(s) 127
FriOI GRGCYC 1 cut(s) 738
FspBI CTAG 1 cut(s) 770
HaeIII GGCC 3 cut(s) 387, 546, 945
HapII CCGG 2 cut(s) 212, 302
Hin1II CATG 6 cut(s) 23, 74, 379, 430, 563, 692
HindIII AAGCTT 1 cut(s) 973
HinfI GANTC 3 cut(s) 308, 509, 521
HpaII CCGG 2 cut(s) 212, 302
HphI GGTGA 2 cut(s) 8, 778
Hpy166II GTNNAC 2 cut(s) 499, 1015
Hpy188I TCNGA 5 cut(s) 181, 406, 520, 526, 867
Hpy188III TCNNGA 5 cut(s) 71, 118, 172, 532, 594
Hpy8I GTNNAC 2 cut(s) 499, 1015
HpyAV CCTTC 1 cut(s) 718
HpyCH4V TGCA 7 cut(s) 199, 222, 266, 364, 559, 692, 889
HpyF10VI GCNNNNNNNGC 7 cut(s) 289, 565, 713, 803, 848, 857, 860
HpyF3I CTNAG 8 cut(s) 159, 358, 403, 592, 653, 790, 834, 864
Hsp92II CATG 6 cut(s) 23, 74, 379, 430, 563, 692
KpnI GGTACC 1 cut(s) 44
Kzo9I GATC 4 cut(s) 13, 67, 90, 492
LmnI GCTCC 3 cut(s) 577, 661, 741
Lsp1109I GCAGC 8 cut(s) 278, 348, 592, 704, 818, 851, 863, 872
LweI GCATC 2 cut(s) 123, 524
MaeI CTAG 1 cut(s) 770
MalI GATC 4 cut(s) 15, 69, 92, 494
MboI GATC 4 cut(s) 13, 67, 90, 492
MboII GAAGA 8 cut(s) 317, 471, 638, 647, 650, 770, 826, 893
MhlI GDGCHC 1 cut(s) 738
MlsI TGGCCA 1 cut(s) 945
MluCI AATT 4 cut(s) 121, 126, 313, 464
MluNI TGGCCA 1 cut(s) 945
MnlI CCTC 9 cut(s) 308, 346, 398, 736, 766, 861, 871, 877, 910
Mox20I TGGCCA 1 cut(s) 945
MscI TGGCCA 1 cut(s) 945
MseI TTAA 2 cut(s) 125, 549
Msp20I TGGCCA 1 cut(s) 945
MspA1I CMGCKG 1 cut(s) 575
MspI CCGG 2 cut(s) 212, 302
MspR9I CCNGG 3 cut(s) 38, 145, 302
Mva1269I GAATGC 1 cut(s) 907
MvaI CCWGG 2 cut(s) 38, 145
MwoI GCNNNNNNNGC 7 cut(s) 289, 565, 713, 803, 848, 857, 860
NciI CCSGG 1 cut(s) 302
NdeI CATATG 2 cut(s) 46, 346
NdeII GATC 4 cut(s) 13, 67, 90, 492
NlaIII CATG 6 cut(s) 23, 74, 379, 430, 563, 692
NlaIV GGNNCC 2 cut(s) 42, 210
NmeAIII GCCGAG 1 cut(s) 302
NspI RCATGY 1 cut(s) 430
PagI TCATGA 1 cut(s) 70
PctI GAATGC 1 cut(s) 907
PfeI GAWTC 3 cut(s) 308, 509, 521
PflFI GACNNNGTC 1 cut(s) 353
PfoI TCCNGGA 2 cut(s) 143, 300
Psp124BI GAGCTC 1 cut(s) 738
Psp6I CCWGG 2 cut(s) 36, 143
PspGI CCWGG 2 cut(s) 36, 143
PspN4I GGNNCC 2 cut(s) 42, 210
PsyI GACNNNGTC 1 cut(s) 353
RsaI GTAC 6 cut(s) 42, 418, 621, 686, 968, 1016
RsaNI GTAC 6 cut(s) 41, 417, 620, 685, 967, 1015
SacI GAGCTC 1 cut(s) 738
SaqAI TTAA 2 cut(s) 125, 549
Sau3AI GATC 4 cut(s) 13, 67, 90, 492
ScaI AGTACT 2 cut(s) 418, 621
ScrFI CCNGG 3 cut(s) 38, 145, 302
SduI GDGCHC 1 cut(s) 738
SfaNI GCATC 2 cut(s) 123, 524
SfcI CTRYAG 2 cut(s) 729, 801
SmlI CTYRAG 3 cut(s) 8, 62, 962
SmoI CTYRAG 3 cut(s) 8, 62, 962
Sse9I AATT 4 cut(s) 121, 126, 313, 464
SsiI CCGC 4 cut(s) 106, 274, 330, 575
SspMI CTAG 1 cut(s) 770
SstI GAGCTC 1 cut(s) 738
StyD4I CCNGG 3 cut(s) 36, 143, 300
StyI CCWWGG 1 cut(s) 81
TaqI TCGA 2 cut(s) 93, 540
TasI AATT 4 cut(s) 121, 126, 313, 464
TatI WGTACW 3 cut(s) 416, 619, 1014
TauI GCSGC 2 cut(s) 276, 332
TfiI GAWTC 3 cut(s) 308, 509, 521
Tru1I TTAA 2 cut(s) 125, 549
Tru9I TTAA 2 cut(s) 125, 549
TscAI CASTG 4 cut(s) 93, 366, 388, 511
TseI GCWGC 8 cut(s) 266, 336, 580, 692, 806, 839, 851, 860
TspDTI ATGAA 5 cut(s) 129, 576, 659, 767, 971
TspRI CASTG 4 cut(s) 93, 366, 388, 511
Tth111I GACNNNGTC 1 cut(s) 353
XapI RAATTY 1 cut(s) 121
XceI RCATGY 1 cut(s) 430
XspI CTAG 1 cut(s) 770
ZrmI AGTACT 2 cut(s) 418, 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.