Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003980

Physical Location & Seq

Genomic Coordinates Reverse (-)

7365477 .. 7366799

Gene Structure 1323 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G22250.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 837 bp

ATGATCAAATCAGAAGCAGATCTCTCCGATGTGATCGTGATTCGTGACGTTTGGGCTTATAATCTCGAGTCAGAGTTTGACCTAATCAGAGGAATCGTCGAAGATTACCCATTCATCTCAATGGATACAGAATTTCCCGGCGTGATTTACAAGGCGGATCTCGACGTTCTCCGCCGTGGAAACCCTAATTATCTCTACAATCTTCTCAAATCCAACGTTGACGCTTTAAGTTTGATCCAAGTCGGTCTTACTCTCTCCGACGCCGACGGAAACCTCCCAGATCTCGGCGGTCAAAAAAACCGTCGATACATCTGGGAATTCAATTTCCGTGACTTCGACGTGGAGCGTGATCCACACGCTCCTGATTCGATCGAGCTACTCCGTCGTCACGGGATCGATTTCGAGCGGAACCGTCGCGAAGGTGTTGAATCGGAGAGGTTCGCGGAGCTGATGATGTCATCGGGGTTGATCTGTAACGAGTCTGTGAGCTGGGTGACGTTTCACAGCGCGTATGATTTTGGTTACCTTGTGAAGATTCTCACGCGCCGGCAGCTTCCGGTGGCGTTGCGTGAGTTTTTAGGGTTGCTTAGAGCTTTCTTCGGTGATAGGGTTTATGATGTGAAACATATTATGAGGTTTTGTGAGCAGAGATTGTATGGTGGTTTGGACCGGGTTGCTCGGTCTCTTGAAGTTAACCGGGCGGTTGGGAAATGTCATCAAGCCGGTTCGGATAGTTTGCTGACGTGGCAGGCGTTTCAGAGGATGAGGGATTTGTATTTCGTTGAGGATGGGGCGGAGAAACACGCCGGAGTTTTGTATGGTTTAGAGGTTTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000175 GO:0000288 GO:0000289 GO:0000291 GO:0000932 GO:0000956 GO:0002213 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004535 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008408 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0017148 GO:0019222 GO:0019439 GO:0030014 GO:0030015 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032268 GO:0032269 GO:0032991 GO:0034248 GO:0034249 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0098542 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1990904 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

CAF1

Protein Analysis

278

Amino Acids

32.17

Weight (kDa)

5.18

Isoelectric Point (pI)

36.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CAF1	PF04857	17 - 143	2.3e-11	CAF1 family ribonuclease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003980)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44260 AT5G22250
fragaria_vesca	FvH4_3g25960 FvH4_6g19860
malus_domestica	MD03G1152000.v1.1 MD03G1152100.v1.1 MD11G1172400.v1.1 MD12G1034100.v1.1
prunus_persica	Prupe.6G139200_v2.0.a1 Prupe.6G139300_v2.0.a1 Prupe.6G139800_v2.0.a1 Prupe.7G098700_v2.0.a1
pyrus_communis	pycom03g10580 pycom05g09910 pycom11g14510 pycom12g03160 pycom14g03120
rosa_chinensis	RchiOBHm_Chr3g0475261 RchiOBHm_Chr5g0047241
rosa_laevigata	RLG00000023855 RLG00000034481
rosa_roxburghii	Rroxscaffold_1G00034130 Rroxscaffold_6G00406420
rosa_rugosa	Rorug03G0147600 Rorug05G0235200
rosa_samantha	Rh3AG197500 Rh3BG227800 Rh3DG223000 Rh5AG314100 Rh5CG349900 Rh5DG333600
rosa_wichuraiana	Rw3G017960 Rw5G029350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	60
AccBSI	CCGCTC	1 cut(s)	406
AccII	CGCG	4 cut(s)	417, 443, 509, 544
AciI	CCGC	7 cut(s)	155, 172, 288, 406, 443, 701, 794
AclI	AACGTT	1 cut(s)	216
AclWI	GGATC	4 cut(s)	165, 229, 344, 401
AcsI	RAATTY	2 cut(s)	131, 317
AcyI	GRCGYC	1 cut(s)	261
AfiI	CCNNNNNNNGG	1 cut(s)	284
AgsI	TTSAA	3 cut(s)	322, 428, 689
AjiI	CACGTC	2 cut(s)	340, 744
AluBI	AGCT	5 cut(s)	376, 448, 489, 553, 593
AluI	AGCT	5 cut(s)	376, 448, 489, 553, 593
Alw26I	GTCTC	1 cut(s)	687
AlwI	GGATC	4 cut(s)	165, 229, 344, 401
Ama87I	CYCGRG	1 cut(s)	65
ApeKI	GCWGC	1 cut(s)	550
ApoI	RAATTY	2 cut(s)	131, 317
ArsI	GACNNNNNNTTYG	2 cut(s)	286, 318
AspLEI	GCGC	2 cut(s)	509, 546
AspS9I	GGNCC	1 cut(s)	667
AsuC2I	CCSGG	3 cut(s)	138, 671, 698
AsuHPI	GGTGA	2 cut(s)	505, 614
AvaI	CYCGRG	1 cut(s)	65
AvaII	GGWCC	1 cut(s)	667
BbvI	GCAGC	1 cut(s)	562
BccI	CCATC	1 cut(s)	782
BceAI	ACGGC	1 cut(s)	159
BciVI	GTATCC	1 cut(s)	118
BclI	TGATCA	1 cut(s)	3
BcnI	CCSGG	3 cut(s)	138, 671, 698
BcoDI	GTCTC	1 cut(s)	687
BfuI	GTATCC	1 cut(s)	118
BglII	AGATCT	2 cut(s)	19, 280
BisI	GCNGC	1 cut(s)	551
BlsI	GCNGC	1 cut(s)	552
Bme1390I	CCNGG	3 cut(s)	138, 671, 698
Bme18I	GGWCC	1 cut(s)	667
BmeT110I	CYCGRG	1 cut(s)	65
BmgBI	CACGTC	2 cut(s)	340, 744
BmgT120I	GGNCC	1 cut(s)	667
BmiI	GGNNCC	1 cut(s)	410
BmrFI	CCNGG	3 cut(s)	138, 671, 698
BpuMI	CCSGG	3 cut(s)	138, 671, 698
Bsa29I	ATCGAT	1 cut(s)	396
BsaHI	GRCGYC	1 cut(s)	261
BsaI	GGTCTC	1 cut(s)	687
BsaJI	CCNNGG	1 cut(s)	175
BsaWI	WCCGGW	1 cut(s)	556
Bsc4I	CCNNNNNNNGG	1 cut(s)	284
Bse118I	RCCGGY	2 cut(s)	546, 722
BseCI	ATCGAT	1 cut(s)	396
BseDI	CCNNGG	1 cut(s)	175
BseGI	GGATG	2 cut(s)	768, 793
BseLI	CCNNNNNNNGG	1 cut(s)	284
BseXI	GCAGC	1 cut(s)	562
BseYI	CCCAGC	1 cut(s)	489
Bsh1236I	CGCG	4 cut(s)	417, 443, 509, 544
Bsh1285I	CGRYCG	1 cut(s)	372
BshVI	ATCGAT	1 cut(s)	396
BsiEI	CGRYCG	1 cut(s)	372
BsiHKCI	CYCGRG	1 cut(s)	65
BsiSI	CCGG	7 cut(s)	138, 547, 557, 670, 697, 723, 807
BslI	CCNNNNNNNGG	1 cut(s)	284
BsmAI	GTCTC	1 cut(s)	687
Bso31I	GGTCTC	1 cut(s)	687
BsoBI	CYCGRG	1 cut(s)	65
Bsp68I	TCGCGA	1 cut(s)	417
BspACI	CCGC	7 cut(s)	155, 172, 288, 406, 443, 701, 794
BspDI	ATCGAT	1 cut(s)	396
BspFNI	CGCG	4 cut(s)	417, 443, 509, 544
BspLI	GGNNCC	1 cut(s)	410
BspPI	GGATC	4 cut(s)	165, 229, 344, 401
BspTNI	GGTCTC	1 cut(s)	687
BsrBI	CCGCTC	1 cut(s)	406
BsrFI	RCCGGY	2 cut(s)	546, 722
BssAI	RCCGGY	2 cut(s)	546, 722
BssECI	CCNNGG	1 cut(s)	175
BssNI	GRCGYC	1 cut(s)	261
Bst4CI	ACNGT	2 cut(s)	302, 413
BstACI	GRCGYC	1 cut(s)	261
BstC8I	GCNNGC	2 cut(s)	548, 750
BstDEI	CTNAG	1 cut(s)	587
BstDSI	CCRYGG	1 cut(s)	175
BstEII	GGTNACC	1 cut(s)	521
BstF5I	GGATG	2 cut(s)	768, 793
BstFNI	CGCG	4 cut(s)	417, 443, 509, 544
BstHHI	GCGC	2 cut(s)	509, 546
BstMAI	GTCTC	1 cut(s)	687
BstMCI	CGRYCG	1 cut(s)	372
BstMWI	GCNNNNNNNGC	2 cut(s)	550, 745
BstPI	GGTNACC	1 cut(s)	521
BstSCI	CCNGG	3 cut(s)	136, 669, 696
BstUI	CGCG	4 cut(s)	417, 443, 509, 544
BstV1I	GCAGC	1 cut(s)	562
BstX2I	RGATCY	3 cut(s)	19, 157, 280
BstYI	RGATCY	3 cut(s)	19, 157, 280
Bsu15I	ATCGAT	1 cut(s)	396
BsuI	GTATCC	1 cut(s)	118
BsuTUI	ATCGAT	1 cut(s)	396
BtgI	CCRYGG	1 cut(s)	175
BtrI	CACGTC	2 cut(s)	340, 744
BtsCI	GGATG	2 cut(s)	768, 793
BtuMI	TCGCGA	1 cut(s)	417
Cac8I	GCNNGC	2 cut(s)	548, 750
CfoI	GCGC	2 cut(s)	509, 546
Cfr10I	RCCGGY	2 cut(s)	546, 722
Cfr13I	GGNCC	1 cut(s)	667
ClaI	ATCGAT	1 cut(s)	396
CseI	GACGC	2 cut(s)	230, 269
CviJI	RGCY	7 cut(s)	56, 376, 448, 489, 553, 593, 722
CviKI_1	RGCY	7 cut(s)	56, 376, 448, 489, 553, 593, 722
DdeI	CTNAG	1 cut(s)	587
EciI	GGCGGA	3 cut(s)	161, 170, 809
Eco31I	GGTCTC	1 cut(s)	687
Eco47I	GGWCC	1 cut(s)	667
Eco88I	CYCGRG	1 cut(s)	65
Eco91I	GGTNACC	1 cut(s)	521
EcoO65I	GGTNACC	1 cut(s)	521
EcoRI	GAATTC	1 cut(s)	317
FaiI	YATR	7 cut(s)	60, 513, 615, 627, 632, 657, 819
FalI	AAGNNNNNCTT	2 cut(s)	231, 263
FbaI	TGATCA	1 cut(s)	3
Fnu4HI	GCNGC	1 cut(s)	551
FokI	GGATG	2 cut(s)	775, 800
Fsp4HI	GCNGC	1 cut(s)	551
GlaI	GCGC	2 cut(s)	508, 545
GluI	GCNGC	1 cut(s)	551
GsaI	CCCAGC	1 cut(s)	493
HapII	CCGG	7 cut(s)	138, 547, 557, 670, 697, 723, 807
HgaI	GACGC	2 cut(s)	230, 269
HhaI	GCGC	2 cut(s)	509, 546
Hin1I	GRCGYC	1 cut(s)	261
Hin6I	GCGC	2 cut(s)	507, 544
HinP1I	GCGC	2 cut(s)	507, 544
HincII	GTYRAC	2 cut(s)	220, 694
HindII	GTYRAC	2 cut(s)	220, 694
HinfI	GANTC	7 cut(s)	40, 68, 93, 365, 428, 479, 535
HpaI	GTTAAC	1 cut(s)	694
HpaII	CCGG	7 cut(s)	138, 547, 557, 670, 697, 723, 807
HphI	GGTGA	2 cut(s)	505, 614
Hpy166II	GTNNAC	2 cut(s)	220, 694
Hpy188I	TCNGA	8 cut(s)	13, 28, 73, 89, 259, 433, 730, 759
Hpy188III	TCNNGA	7 cut(s)	37, 44, 65, 161, 362, 416, 686
Hpy8I	GTNNAC	2 cut(s)	220, 694
Hpy99I	CGWCG	8 cut(s)	101, 167, 263, 269, 306, 341, 387, 417
HpyAV	CCTTC	1 cut(s)	413
HpyCH4III	ACNGT	2 cut(s)	302, 413
HpyCH4IV	ACGT	6 cut(s)	48, 165, 216, 339, 497, 743
HpyF10VI	GCNNNNNNNGC	2 cut(s)	550, 745
HpyF3I	CTNAG	1 cut(s)	587
HpySE526I	ACGT	6 cut(s)	48, 165, 216, 339, 497, 743
Hsp92I	GRCGYC	1 cut(s)	261
HspAI	GCGC	2 cut(s)	507, 544
KroI	GCCGGC	1 cut(s)	546
KroNI	GCCGGC	1 cut(s)	548
Ksp22I	TGATCA	1 cut(s)	3
KspAI	GTTAAC	1 cut(s)	694
LmnI	GCTCC	3 cut(s)	343, 364, 445
Lsp1109I	GCAGC	1 cut(s)	562
MaeII	ACGT	6 cut(s)	48, 165, 216, 339, 497, 743
MaeIII	GTNAC	6 cut(s)	44, 329, 386, 473, 493, 521
MbiI	CCGCTC	1 cut(s)	406
MboII	GAAGA	4 cut(s)	113, 194, 544, 589
MflI	RGATCY	3 cut(s)	19, 157, 280
MluCI	AATT	4 cut(s)	131, 187, 317, 322
MlyI	GAGTC	2 cut(s)	77, 488
MmeI	TCCRAC	2 cut(s)	237, 282
MnlI	CCTC	8 cut(s)	83, 284, 429, 627, 753, 759, 778, 820
MroNI	GCCGGC	1 cut(s)	546
MseI	TTAA	2 cut(s)	227, 693
MslI	CAYNNNNRTG	1 cut(s)	119
MspI	CCGG	7 cut(s)	138, 547, 557, 670, 697, 723, 807
MspR9I	CCNGG	3 cut(s)	138, 671, 698
MvnI	CGCG	4 cut(s)	417, 443, 509, 544
MwoI	GCNNNNNNNGC	2 cut(s)	550, 745
NaeI	GCCGGC	1 cut(s)	548
NciI	CCSGG	3 cut(s)	138, 671, 698
NgoMIV	GCCGGC	1 cut(s)	546
NlaIV	GGNNCC	1 cut(s)	410
NmeAIII	GCCGAG	1 cut(s)	264
NmuCI	GTSAC	4 cut(s)	44, 329, 386, 493
NruI	TCGCGA	1 cut(s)	417
PaeR7I	CTCGAG	1 cut(s)	65
PcsI	WCGNNNNNNNCGW	1 cut(s)	749
PdiI	GCCGGC	1 cut(s)	548
PfeI	GAWTC	5 cut(s)	40, 93, 365, 428, 535
PkrI	GCNGC	1 cut(s)	552
Ple19I	CGATCG	1 cut(s)	372
PleI	GAGTC	2 cut(s)	76, 487
PpsI	GAGTC	2 cut(s)	76, 487
PsiI	TTATAA	1 cut(s)	60
Psp1406I	AACGTT	1 cut(s)	216
PspEI	GGTNACC	1 cut(s)	521
PspFI	CCCAGC	1 cut(s)	489
PspN4I	GGNNCC	1 cut(s)	410
PspPI	GGNCC	1 cut(s)	667
PsuI	RGATCY	3 cut(s)	19, 157, 280
PvuI	CGATCG	1 cut(s)	372
RruI	TCGCGA	1 cut(s)	417
RseI	CAYNNNNRTG	1 cut(s)	119
SaqAI	TTAA	2 cut(s)	227, 693
SatI	GCNGC	1 cut(s)	551
Sau96I	GGNCC	1 cut(s)	667
SchI	GAGTC	2 cut(s)	77, 488
ScrFI	CCNGG	3 cut(s)	138, 671, 698
Sfr274I	CTCGAG	1 cut(s)	65
SinI	GGWCC	1 cut(s)	667
SlaI	CTCGAG	1 cut(s)	65
SmiMI	CAYNNNNRTG	1 cut(s)	119
SmlI	CTYRAG	1 cut(s)	65
SmoI	CTYRAG	1 cut(s)	65
Sse9I	AATT	4 cut(s)	131, 187, 317, 322
SsiI	CCGC	7 cut(s)	155, 172, 288, 406, 443, 701, 794
StyD4I	CCNGG	3 cut(s)	136, 669, 696
TaaI	ACNGT	2 cut(s)	302, 413
TaiI	ACGT	6 cut(s)	51, 168, 219, 342, 500, 746
TaqI	TCGA	9 cut(s)	66, 99, 162, 304, 336, 368, 372, 396, 402
TaqII	GACCGA	2 cut(s)	233, 669
TasI	AATT	4 cut(s)	131, 187, 317, 322
TfiI	GAWTC	5 cut(s)	40, 93, 365, 428, 535
Tru1I	TTAA	2 cut(s)	227, 693
Tru9I	TTAA	2 cut(s)	227, 693
TseFI	GTSAC	4 cut(s)	44, 329, 386, 493
TseI	GCWGC	1 cut(s)	550
Tsp45I	GTSAC	4 cut(s)	44, 329, 386, 493
TspDTI	ATGAA	1 cut(s)	103
TspGWI	ACGGA	3 cut(s)	282, 317, 371
VpaK11BI	GGWCC	1 cut(s)	667
XapI	RAATTY	2 cut(s)	131, 317
XhoI	CTCGAG	1 cut(s)	65

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.