Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003980

Physical Location & Seq

Genomic Coordinates Forward (+)

3741371 .. 3743032

Gene Structure 1662 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1034100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 849 bp

ATGTCATCATCCGGCCCCCCTCCACCCAAGCCTCCGCCGCCACGAGACATCCTGATCCGCTCGGTGTGGTCCGATAACCTCGAGTCCGAGTTCGAACTGATCCGATCCGTCATCGACGATTACCCTTTGATTTCCATGGATACCGAATTCCCGGGCGTCGTGGTCCGACCCGACTCCAACGATCCCAATTTCTACCACCGCGAGCCCGCCGCGCACTACCTCATGCTCAAGGCCAACGTCGATCGCCTCAATCTGATCCAGGTCGGCCTCACCCTCTCGGACGACAGCGGAAATCTGCCGGATTTGGGGACCAAGAATCTGTATATATGGGAATTCAATTTCCGGGACTTCGATGTCGCGCGGGACGCCCACGCGCACGACTCGGTCGAGTTGCTTCGCCGCCAGGGGATCGATTTCGAGCGGAACCGGGATTTCGGGGTGGACTCGGTCCGGTTCGCGGAGCTGATGATGTCGTCGGGGCTCGTCTGCAACGACGCCGTAAACTGGGTCACGTTCCACAGCGCCTACGATTTCGGGTACCTGGTCAAGATGTTGACTCAGCGAGTCTTGCCGGACGAGTTGTCGGTGTTCTTGAATTACGTGAGGGTTTTCTTTGGGTCTGGAGTGTACGACGTGAAGCACCTGACGAGGTTCTGCGCCGCCCTCTATGGTGGGCTGGATCGGGTCTGCCAGACGCTGAATGTGGGTCGGGTTGCCGGTAAGAGCCACCAGGCCGGGTCGGATAGCCTGCTAACCCTGCACGCGTTCCAGAGGATCAGAGAGGTCTATTTTCCGAAGGATGGGATCGTGAAGTACGCCGGCGTTTTGTATGGTCTAGAGGTTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000175 GO:0000288 GO:0000289 GO:0000291 GO:0000932 GO:0000956 GO:0002213 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004535 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008408 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0017148 GO:0019222 GO:0019439 GO:0030014 GO:0030015 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032268 GO:0032269 GO:0032991 GO:0034248 GO:0034249 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0098542 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1990904 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

CAF1

Protein Analysis

283

Amino Acids

31.9

Weight (kDa)

5.35

Isoelectric Point (pI)

32.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CAF1	PF04857	22 - 147	1.4e-11	CAF1 family ribonuclease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003980)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44260 AT5G22250
fragaria_vesca	FvH4_3g25960 FvH4_6g19860
malus_domestica	MD03G1152000.v1.1 MD03G1152100.v1.1 MD11G1172400.v1.1 MD12G1034100.v1.1
prunus_persica	Prupe.6G139200_v2.0.a1 Prupe.6G139300_v2.0.a1 Prupe.6G139800_v2.0.a1 Prupe.7G098700_v2.0.a1
pyrus_communis	pycom03g10580 pycom05g09910 pycom11g14510 pycom12g03160 pycom14g03120
rosa_chinensis	RchiOBHm_Chr3g0475261 RchiOBHm_Chr5g0047241
rosa_laevigata	RLG00000023855 RLG00000034481
rosa_roxburghii	Rroxscaffold_1G00034130 Rroxscaffold_6G00406420
rosa_rugosa	Rorug03G0147600 Rorug05G0235200
rosa_samantha	Rh3AG197500 Rh3BG227800 Rh3DG223000 Rh5AG314100 Rh5CG349900 Rh5DG333600
rosa_wichuraiana	Rw3G017960 Rw5G029350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	353
Acc65I	GGTACC	1 cut(s)	537
AccB1I	GGYRCC	1 cut(s)	537
AccBSI	CCGCTC	2 cut(s)	60, 421
AccII	CGCG	7 cut(s)	201, 212, 359, 361, 374, 458, 764
AclWI	GGATC	9 cut(s)	49, 94, 99, 176, 250, 416, 687, 782, 812
AcsI	RAATTY	2 cut(s)	146, 332
AcyI	GRCGYC	3 cut(s)	156, 366, 495
AfaI	GTAC	3 cut(s)	539, 629, 815
AfiI	CCNNNNNNNGG	4 cut(s)	305, 457, 504, 800
AflIII	ACRYGT	1 cut(s)	762
AgsI	TTSAA	2 cut(s)	337, 595
AhdI	GACNNNNNGTC	1 cut(s)	580
AjiI	CACGTC	1 cut(s)	634
AjnI	CCWGG	4 cut(s)	258, 402, 540, 729
AluBI	AGCT	1 cut(s)	463
AluI	AGCT	1 cut(s)	463
Alw26I	GTCTC	1 cut(s)	39
AlwI	GGATC	9 cut(s)	49, 94, 99, 176, 250, 416, 687, 782, 812
AlwNI	CAGNNNCTG	1 cut(s)	697
Ama87I	CYCGRG	2 cut(s)	80, 151
AoxI	GGCC	4 cut(s)	13, 231, 265, 732
ApoI	RAATTY	2 cut(s)	146, 332
Asp718I	GGTACC	1 cut(s)	537
AspLEI	GCGC	5 cut(s)	214, 361, 376, 524, 659
AspS9I	GGNCC	5 cut(s)	14, 69, 163, 309, 448
AsuC2I	CCSGG	5 cut(s)	152, 153, 344, 428, 736
AsuHPI	GGTGA	1 cut(s)	262
AsuII	TTCGAA	1 cut(s)	93
AvaI	CYCGRG	2 cut(s)	80, 151
AvaII	GGWCC	4 cut(s)	69, 163, 309, 448
BanI	GGYRCC	1 cut(s)	537
BanII	GRGCYC	2 cut(s)	207, 483
BauI	CACGAG	1 cut(s)	42
BccI	CCATC	1 cut(s)	794
BceAI	ACGGC	1 cut(s)	482
BciT130I	CCWGG	4 cut(s)	260, 404, 542, 731
BciVI	GTATCC	1 cut(s)	133
BcnI	CCSGG	5 cut(s)	152, 153, 344, 428, 736
BcoDI	GTCTC	1 cut(s)	39
BfaI	CTAG	1 cut(s)	836
BfoI	RGCGCY	1 cut(s)	525
BfuI	GTATCC	1 cut(s)	133
BisI	GCNGC	4 cut(s)	38, 210, 400, 660
BlsI	GCNGC	4 cut(s)	39, 211, 401, 661
Bme1390I	CCNGG	9 cut(s)	152, 153, 260, 344, 404, 428, 542, 731, 736
Bme18I	GGWCC	4 cut(s)	69, 163, 309, 448
BmeRI	GACNNNNNGTC	1 cut(s)	580
BmeT110I	CYCGRG	2 cut(s)	80, 151
BmgBI	CACGTC	1 cut(s)	634
BmgT120I	GGNCC	5 cut(s)	14, 69, 163, 309, 448
BmiI	GGNNCC	4 cut(s)	16, 310, 425, 539
BmrFI	CCNGG	9 cut(s)	152, 153, 260, 344, 404, 428, 542, 731, 736
BmrI	ACTGGG	1 cut(s)	514
BmuI	ACTGGG	1 cut(s)	514
BpmI	CTGGAG	1 cut(s)	642
Bpu14I	TTCGAA	1 cut(s)	93
BpuEI	CTTGAG	1 cut(s)	212
BpuMI	CCSGG	5 cut(s)	152, 153, 344, 428, 736
Bsa29I	ATCGAT	1 cut(s)	411
BsaAI	YACGTR	1 cut(s)	601
BsaHI	GRCGYC	3 cut(s)	156, 366, 495
BsaJI	CCNNGG	3 cut(s)	135, 151, 403
BsaWI	WCCGGW	1 cut(s)	450
Bsc4I	CCNNNNNNNGG	4 cut(s)	305, 457, 504, 800
Bse118I	RCCGGY	2 cut(s)	716, 818
Bse1I	ACTGG	1 cut(s)	509
BseBI	CCWGG	4 cut(s)	260, 404, 542, 731
BseCI	ATCGAT	1 cut(s)	411
BseDI	CCNNGG	3 cut(s)	135, 151, 403
BseGI	GGATG	3 cut(s)	8, 48, 805
BseLI	CCNNNNNNNGG	4 cut(s)	305, 457, 504, 800
BseMII	CTCAG	1 cut(s)	572
BseNI	ACTGG	1 cut(s)	509
BsgI	GTGCAG	1 cut(s)	743
Bsh1236I	CGCG	7 cut(s)	201, 212, 359, 361, 374, 458, 764
Bsh1285I	CGRYCG	2 cut(s)	244, 387
BshFI	GGCC	4 cut(s)	15, 233, 267, 734
BshNI	GGYRCC	1 cut(s)	537
BshVI	ATCGAT	1 cut(s)	411
BsiEI	CGRYCG	2 cut(s)	244, 387
BsiHKCI	CYCGRG	2 cut(s)	80, 151
BslFI	GGGAC	3 cut(s)	322, 359, 377
BslI	CCNNNNNNNGG	4 cut(s)	305, 457, 504, 800
BsmAI	GTCTC	1 cut(s)	39
BsmFI	GGGAC	3 cut(s)	322, 359, 377
BsnI	GGCC	4 cut(s)	15, 233, 267, 734
BsoBI	CYCGRG	2 cut(s)	80, 151
Bsp119I	TTCGAA	1 cut(s)	93
Bsp1286I	GDGCHC	2 cut(s)	207, 483
Bsp19I	CCATGG	1 cut(s)	135
BspANI	GGCC	4 cut(s)	15, 233, 267, 734
BspCNI	CTCAG	1 cut(s)	571
BspDI	ATCGAT	1 cut(s)	411
BspFNI	CGCG	7 cut(s)	201, 212, 359, 361, 374, 458, 764
BspLI	GGNNCC	4 cut(s)	16, 310, 425, 539
BspPI	GGATC	9 cut(s)	49, 94, 99, 176, 250, 416, 687, 782, 812
BspT104I	TTCGAA	1 cut(s)	93
BspT107I	GGYRCC	1 cut(s)	537
BsrBI	CCGCTC	2 cut(s)	60, 421
BsrFI	RCCGGY	2 cut(s)	716, 818
BsrI	ACTGG	1 cut(s)	509
BssAI	RCCGGY	2 cut(s)	716, 818
BssECI	CCNNGG	3 cut(s)	135, 151, 403
BssNI	GRCGYC	3 cut(s)	156, 366, 495
BssSI	CACGAG	1 cut(s)	42
BssT1I	CCWWGG	1 cut(s)	135
Bst2BI	CACGAG	1 cut(s)	42
Bst2UI	CCWGG	4 cut(s)	260, 404, 542, 731
BstACI	GRCGYC	3 cut(s)	156, 366, 495
BstBAI	YACGTR	1 cut(s)	601
BstBI	TTCGAA	1 cut(s)	93
BstC8I	GCNNGC	5 cut(s)	203, 207, 749, 762, 820
BstDEI	CTNAG	1 cut(s)	558
BstDSI	CCRYGG	1 cut(s)	135
BstF5I	GGATG	3 cut(s)	8, 48, 805
BstFNI	CGCG	7 cut(s)	201, 212, 359, 361, 374, 458, 764
BstH2I	RGCGCY	1 cut(s)	525
BstHHI	GCGC	5 cut(s)	214, 361, 376, 524, 659
BstMAI	GTCTC	1 cut(s)	39
BstMCI	CGRYCG	2 cut(s)	244, 387
BstMWI	GCNNNNNNNGC	5 cut(s)	37, 211, 365, 568, 757
BstNI	CCWGG	4 cut(s)	260, 404, 542, 731
BstSCI	CCNGG	9 cut(s)	150, 151, 258, 342, 402, 426, 540, 729, 734
BstUI	CGCG	7 cut(s)	201, 212, 359, 361, 374, 458, 764
Bsu15I	ATCGAT	1 cut(s)	411
BsuI	GTATCC	1 cut(s)	133
BsuRI	GGCC	4 cut(s)	15, 233, 267, 734
BsuTUI	ATCGAT	1 cut(s)	411
BtgI	CCRYGG	1 cut(s)	135
BtrI	CACGTC	1 cut(s)	634
BtsCI	GGATG	3 cut(s)	8, 48, 805
Cac8I	GCNNGC	5 cut(s)	203, 207, 749, 762, 820
CaiI	CAGNNNCTG	1 cut(s)	697
CfoI	GCGC	5 cut(s)	214, 361, 376, 524, 659
Cfr10I	RCCGGY	2 cut(s)	716, 818
Cfr13I	GGNCC	5 cut(s)	14, 69, 163, 309, 448
Cfr9I	CCCGGG	1 cut(s)	151
ClaI	ATCGAT	1 cut(s)	411
CpoI	CGGWCCG	1 cut(s)	448
CseI	GACGC	4 cut(s)	145, 374, 503, 703
CsiI	ACCWGGT	1 cut(s)	540
Csp6I	GTAC	3 cut(s)	538, 628, 814
CspI	CGGWCCG	1 cut(s)	448
CviAII	CATG	2 cut(s)	136, 223
CviQI	GTAC	3 cut(s)	538, 628, 814
DdeI	CTNAG	1 cut(s)	558
DrdI	GACNNNNNNGTC	1 cut(s)	353
DriI	GACNNNNNGTC	1 cut(s)	580
DseDI	GACNNNNNNGTC	1 cut(s)	353
Eam1105I	GACNNNNNGTC	1 cut(s)	580
EciI	GGCGGA	1 cut(s)	24
Eco130I	CCWWGG	1 cut(s)	135
Eco24I	GRGCYC	2 cut(s)	207, 483
Eco47I	GGWCC	4 cut(s)	69, 163, 309, 448
Eco88I	CYCGRG	2 cut(s)	80, 151
EcoRI	GAATTC	2 cut(s)	146, 332
EcoRII	CCWGG	4 cut(s)	258, 402, 540, 729
EcoT14I	CCWWGG	1 cut(s)	135
EcoT38I	GRGCYC	2 cut(s)	207, 483
ErhI	CCWWGG	1 cut(s)	135
FaeI	CATG	2 cut(s)	139, 226
FaiI	YATR	7 cut(s)	137, 224, 324, 326, 328, 669, 831
FaqI	GGGAC	3 cut(s)	322, 359, 377
FatI	CATG	2 cut(s)	135, 222
FauI	CCCGC	2 cut(s)	214, 354
Fnu4HI	GCNGC	4 cut(s)	38, 210, 400, 660
FokI	GGATG	2 cut(s)	35, 812
FriOI	GRGCYC	2 cut(s)	207, 483
Fsp4HI	GCNGC	4 cut(s)	38, 210, 400, 660
FspBI	CTAG	1 cut(s)	836
GlaI	GCGC	5 cut(s)	213, 360, 375, 523, 658
GluI	GCNGC	4 cut(s)	38, 210, 400, 660
GsuI	CTGGAG	1 cut(s)	642
HaeII	RGCGCY	1 cut(s)	525
HaeIII	GGCC	4 cut(s)	15, 233, 267, 734
HgaI	GACGC	4 cut(s)	145, 374, 503, 703
HhaI	GCGC	5 cut(s)	214, 361, 376, 524, 659
Hin1I	GRCGYC	3 cut(s)	156, 366, 495
Hin1II	CATG	2 cut(s)	139, 226
Hin6I	GCGC	5 cut(s)	212, 359, 374, 522, 657
HinP1I	GCGC	5 cut(s)	212, 359, 374, 522, 657
HincII	GTYRAC	1 cut(s)	555
HindII	GTYRAC	1 cut(s)	555
HinfI	GANTC	7 cut(s)	83, 173, 316, 380, 443, 556, 564
HphI	GGTGA	1 cut(s)	262
Hpy166II	GTNNAC	4 cut(s)	442, 502, 555, 628
Hpy188I	TCNGA	9 cut(s)	73, 88, 104, 167, 255, 280, 742, 779, 795
Hpy188III	TCNNGA	8 cut(s)	52, 547, 592, 621, 769, 808, 836, 846
Hpy8I	GTNNAC	4 cut(s)	442, 502, 555, 628
Hpy99I	CGWCG	6 cut(s)	119, 161, 242, 478, 497, 635
HpyAV	CCTTC	1 cut(s)	790
HpyCH4IV	ACGT	4 cut(s)	237, 512, 600, 633
HpyCH4V	TGCA	2 cut(s)	489, 760
HpyF10VI	GCNNNNNNNGC	5 cut(s)	37, 211, 365, 568, 757
HpyF3I	CTNAG	1 cut(s)	558
HpySE526I	ACGT	4 cut(s)	237, 512, 600, 633
Hsp92I	GRCGYC	3 cut(s)	156, 366, 495
Hsp92II	CATG	2 cut(s)	139, 226
HspAI	GCGC	5 cut(s)	212, 359, 374, 522, 657
KpnI	GGTACC	1 cut(s)	541
KroI	GCCGGC	1 cut(s)	818
KroNI	GCCGGC	1 cut(s)	820
LmnI	GCTCC	1 cut(s)	460
MabI	ACCWGGT	1 cut(s)	540
MaeI	CTAG	1 cut(s)	836
MaeII	ACGT	4 cut(s)	237, 512, 600, 633
MaeIII	GTNAC	1 cut(s)	508
MbiI	CCGCTC	2 cut(s)	60, 421
MhlI	GDGCHC	2 cut(s)	207, 483
MluCI	AATT	5 cut(s)	146, 187, 332, 337, 595
MluI	ACGCGT	1 cut(s)	762
MlyI	GAGTC	6 cut(s)	92, 167, 374, 437, 550, 573
MmeI	TCCRAC	3 cut(s)	190, 201, 720
MreI	CGCCGGCG	1 cut(s)	818
MroNI	GCCGGC	1 cut(s)	818
MspA1I	CMGCKG	1 cut(s)	288
MspR9I	CCNGG	9 cut(s)	152, 153, 260, 344, 404, 428, 542, 731, 736
MvaI	CCWGG	4 cut(s)	260, 404, 542, 731
MvnI	CGCG	7 cut(s)	201, 212, 359, 361, 374, 458, 764
MwoI	GCNNNNNNNGC	5 cut(s)	37, 211, 365, 568, 757
NaeI	GCCGGC	1 cut(s)	820
NciI	CCSGG	5 cut(s)	152, 153, 344, 428, 736
NcoI	CCATGG	1 cut(s)	135
NgoMIV	GCCGGC	1 cut(s)	818
NlaIII	CATG	2 cut(s)	139, 226
NlaIV	GGNNCC	4 cut(s)	16, 310, 425, 539
NmuCI	GTSAC	1 cut(s)	508
NspV	TTCGAA	1 cut(s)	93
PaeR7I	CTCGAG	1 cut(s)	80
PcsI	WCGNNNNNNNCGW	2 cut(s)	384, 489
PdiI	GCCGGC	1 cut(s)	820
PfeI	GAWTC	1 cut(s)	316
PflFI	GACNNNGTC	2 cut(s)	383, 446
PfoI	TCCNGGA	1 cut(s)	342
PkrI	GCNGC	4 cut(s)	39, 211, 401, 661
Ple19I	CGATCG	1 cut(s)	244
PleI	GAGTC	6 cut(s)	91, 167, 374, 437, 550, 572
PpsI	GAGTC	6 cut(s)	91, 167, 374, 437, 550, 572
Ppu21I	YACGTR	1 cut(s)	601
Psp6I	CCWGG	4 cut(s)	258, 402, 540, 729
PspGI	CCWGG	4 cut(s)	258, 402, 540, 729
PspN4I	GGNNCC	4 cut(s)	16, 310, 425, 539
PspPI	GGNCC	5 cut(s)	14, 69, 163, 309, 448
PspXI	VCTCGAGB	1 cut(s)	80
PstNI	CAGNNNCTG	1 cut(s)	697
PsyI	GACNNNGTC	2 cut(s)	383, 446
PvuI	CGATCG	1 cut(s)	244
RsaI	GTAC	3 cut(s)	539, 629, 815
RsaNI	GTAC	3 cut(s)	538, 628, 814
Rsr2I	CGGWCCG	1 cut(s)	448
RsrII	CGGWCCG	1 cut(s)	448
SatI	GCNGC	4 cut(s)	38, 210, 400, 660
Sau96I	GGNCC	5 cut(s)	14, 69, 163, 309, 448
SchI	GAGTC	6 cut(s)	92, 167, 374, 437, 550, 573
ScrFI	CCNGG	9 cut(s)	152, 153, 260, 344, 404, 428, 542, 731, 736
SduI	GDGCHC	2 cut(s)	207, 483
SexAI	ACCWGGT	1 cut(s)	540
Sfr274I	CTCGAG	1 cut(s)	80
SfuI	TTCGAA	1 cut(s)	93
SgrAI	CRCCGGYG	1 cut(s)	818
SinI	GGWCC	4 cut(s)	69, 163, 309, 448
SlaI	CTCGAG	1 cut(s)	80
SmaI	CCCGGG	1 cut(s)	153
SmlI	CTYRAG	2 cut(s)	80, 227
SmoI	CTYRAG	2 cut(s)	80, 227
Sse9I	AATT	5 cut(s)	146, 187, 332, 337, 595
SspMI	CTAG	1 cut(s)	836
StyD4I	CCNGG	9 cut(s)	150, 151, 258, 342, 402, 426, 540, 729, 734
StyI	CCWWGG	1 cut(s)	135
TaiI	ACGT	4 cut(s)	240, 515, 603, 636
TaqI	TCGA	8 cut(s)	81, 93, 114, 240, 351, 387, 411, 417
TaqII	GACCGA	2 cut(s)	373, 436
TasI	AATT	5 cut(s)	146, 187, 332, 337, 595
TauI	GCSGC	4 cut(s)	40, 212, 402, 662
TfiI	GAWTC	1 cut(s)	316
TseFI	GTSAC	1 cut(s)	508
Tsp45I	GTSAC	1 cut(s)	508
TspGWI	ACGGA	1 cut(s)	97
TspMI	CCCGGG	1 cut(s)	151
Tth111I	GACNNNGTC	2 cut(s)	383, 446
VpaK11BI	GGWCC	4 cut(s)	69, 163, 309, 448
XapI	RAATTY	2 cut(s)	146, 332
XbaI	TCTAGA	1 cut(s)	835
XhoI	CTCGAG	1 cut(s)	80
XmaI	CCCGGG	1 cut(s)	151
XspI	CTAG	1 cut(s)	836

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.