Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0003980

Physical Location & Seq

Genomic Coordinates Forward (+)

17271202 .. 17272053

Gene Structure 852 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1152100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 852 bp

ATGTACGGCGACCAAGATCCGACGACTCCGATCCAAAAACCCATCGTGATCCGTGAAGTCTGGGCCCACAATCTGGAATCCGAATTCGAGCTCATCCAAAACGTCATCGACGCCTACCCTTTCATCTCCATGGACACCGAGTTTCCCGGCGTCGTCTTCCGGCCCCCCGTCGACCCTACCAGTCCCTACTCCCGCCAGACCCTCCGGCCCTCCGATCACTACAGGATTCTCAAATCCAACGTCGACGCCCTCAGCCTCATCCAGGTAGGACTCACCCTCTCCGACTCCCGCGGTAATCTCCCCGACCTCGGCTCCCCCACGCGCTTCATCTGGGAGTTCAACTTCAACGACTTCGACGTCGCGCGTGACCCCCACGCTCCAGACTCCATCGAACTCCTCCGCCGCCAGGGCATCGACTTCGACCGCAACCGCTCCCACGGCGTCGACTCCTCGCGCTTCGCGGAGCTGATGATGTCGTCGGGACTCGTCTGCAACGACTCGGTGAGTTGGGTCACGTTTCACAGCGCCTACGACTTCGGGTACCTGGTGAAGATTCTCACGCGCCGGGACTTGCCCAGTGGCTTGAACGAATTTCTGAAAATTCTGAGGGTCTTTTTCGGAAATAGGGTTTATGACGTCAAGCACATGATGCGATTTTGCAAAAGCTTGTACGGCGGGTTGGACCGGGTTGCTAGAACCTTAGAGGTGGACCGGGCGGTCGGGAAGTGCCACCAGGCCGGTTCAGATAGTTTGTTGACATGGCATGCGTTCCAGAAGATGAGGGATGTGTACTTTGTGCATGGTGGGCCCGAGCAACACGCTGGAGTATTGTATGGATTGGAAGTGTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000175 GO:0000288 GO:0000289 GO:0000291 GO:0000932 GO:0000956 GO:0002213 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004535 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008408 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0017148 GO:0019222 GO:0019439 GO:0030014 GO:0030015 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032268 GO:0032269 GO:0032991 GO:0034248 GO:0034249 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0098542 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1990904 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

CAF1

Protein Analysis

284

Amino Acids

32.37

Weight (kDa)

5.92

Isoelectric Point (pI)

41.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CAF1	PF04857	20 - 148	1.1e-10	CAF1 family ribonuclease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003980)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44260 AT5G22250
fragaria_vesca	FvH4_3g25960 FvH4_6g19860
malus_domestica	MD03G1152000.v1.1 MD03G1152100.v1.1 MD11G1172400.v1.1 MD12G1034100.v1.1
prunus_persica	Prupe.6G139200_v2.0.a1 Prupe.6G139300_v2.0.a1 Prupe.6G139800_v2.0.a1 Prupe.7G098700_v2.0.a1
pyrus_communis	pycom03g10580 pycom05g09910 pycom11g14510 pycom12g03160 pycom14g03120
rosa_chinensis	RchiOBHm_Chr3g0475261 RchiOBHm_Chr5g0047241
rosa_laevigata	RLG00000023855 RLG00000034481
rosa_roxburghii	Rroxscaffold_1G00034130 Rroxscaffold_6G00406420
rosa_rugosa	Rorug03G0147600 Rorug05G0235200
rosa_samantha	Rh3AG197500 Rh3BG227800 Rh3DG223000 Rh5AG314100 Rh5CG349900 Rh5DG333600
rosa_wichuraiana	Rw3G017960 Rw5G029350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	356, 716
AatII	GACGTC	2 cut(s)	360, 639
Acc65I	GGTACC	1 cut(s)	540
AccB1I	GGYRCC	1 cut(s)	540
AccB7I	CCANNNNNTGG	1 cut(s)	73
AccBSI	CCGCTC	1 cut(s)	432
AccI	GTMKAC	3 cut(s)	171, 243, 444
AccII	CGCG	7 cut(s)	291, 322, 362, 364, 454, 461, 562
AclWI	GGATC	3 cut(s)	11, 25, 43
AcsI	RAATTY	3 cut(s)	83, 590, 600
AcyI	GRCGYC	6 cut(s)	111, 150, 246, 357, 441, 636
AfaI	GTAC	4 cut(s)	5, 542, 671, 791
AfiI	CCNNNNNNNGG	4 cut(s)	73, 262, 308, 406
AgsI	TTSAA	3 cut(s)	340, 346, 586
AjnI	CCWGG	4 cut(s)	261, 405, 543, 732
AluBI	AGCT	3 cut(s)	91, 466, 666
AluI	AGCT	3 cut(s)	91, 466, 666
Alw21I	GWGCWC	1 cut(s)	93
AlwI	GGATC	3 cut(s)	11, 25, 43
Ama87I	CYCGRG	1 cut(s)	809
AoxI	GGCC	5 cut(s)	63, 161, 206, 735, 806
ApaI	GGGCCC	2 cut(s)	67, 810
ApoI	RAATTY	3 cut(s)	83, 590, 600
ArsI	GACNNNNNNTTYG	2 cut(s)	225, 257
Asp718I	GGTACC	1 cut(s)	540
AspLEI	GCGC	5 cut(s)	324, 364, 456, 527, 564
AspS9I	GGNCC	8 cut(s)	63, 64, 162, 207, 682, 709, 806, 807
AsuC2I	CCSGG	4 cut(s)	147, 566, 686, 713
AsuHPI	GGTGA	3 cut(s)	265, 514, 559
AvaI	CYCGRG	1 cut(s)	809
AvaII	GGWCC	2 cut(s)	682, 709
BaeGI	GKGCMC	2 cut(s)	67, 810
BanI	GGYRCC	1 cut(s)	540
BanII	GRGCYC	3 cut(s)	67, 93, 810
BbsI	GAAGAC	1 cut(s)	148
Bbv12I	GWGCWC	1 cut(s)	93
BbvCI	CCTCAGC	1 cut(s)	251
BccI	CCATC	2 cut(s)	50, 395
BceAI	ACGGC	3 cut(s)	22, 454, 688
BcgI	CGANNNNNNTGC	2 cut(s)	400, 434
BciT130I	CCWGG	4 cut(s)	263, 407, 545, 734
BcnI	CCSGG	4 cut(s)	147, 566, 686, 713
BfaI	CTAG	1 cut(s)	693
BfmI	CTRYAG	1 cut(s)	220
BfoI	RGCGCY	1 cut(s)	528
BglI	GCCNNNNNGGC	1 cut(s)	408
BisI	GCNGC	1 cut(s)	403
BlsI	GCNGC	1 cut(s)	404
Bme1390I	CCNGG	8 cut(s)	147, 263, 407, 545, 566, 686, 713, 734
Bme18I	GGWCC	2 cut(s)	682, 709
BmeT110I	CYCGRG	1 cut(s)	809
BmgT120I	GGNCC	8 cut(s)	63, 64, 162, 207, 682, 709, 806, 807
BmiI	GGNNCC	5 cut(s)	65, 164, 313, 542, 808
BmrFI	CCNGG	8 cut(s)	147, 263, 407, 545, 566, 686, 713, 734
BmrI	ACTGGG	1 cut(s)	570
BmsI	GCATC	2 cut(s)	420, 639
BmuI	ACTGGG	1 cut(s)	570
BpiI	GAAGAC	1 cut(s)	148
BpmI	CTGGAG	2 cut(s)	363, 843
Bpu10I	CCTNAGC	1 cut(s)	251
BpuMI	CCSGG	4 cut(s)	147, 566, 686, 713
BsaHI	GRCGYC	6 cut(s)	111, 150, 246, 357, 441, 636
BsaJI	CCNNGG	5 cut(s)	129, 289, 307, 406, 436
BsaXI	ACNNNNNCTCC	2 cut(s)	296, 326
Bsc4I	CCNNNNNNNGG	4 cut(s)	73, 262, 308, 406
Bse118I	RCCGGY	1 cut(s)	737
Bse1I	ACTGG	2 cut(s)	180, 576
BseBI	CCWGG	4 cut(s)	263, 407, 545, 734
BseDI	CCNNGG	5 cut(s)	129, 289, 307, 406, 436
BseGI	GGATG	3 cut(s)	93, 258, 790
BseLI	CCNNNNNNNGG	4 cut(s)	73, 262, 308, 406
BseMII	CTCAG	2 cut(s)	265, 596
BseNI	ACTGG	2 cut(s)	180, 576
BseRI	GAGGAG	2 cut(s)	386, 439
BseSI	GKGCMC	2 cut(s)	67, 810
Bsh1236I	CGCG	7 cut(s)	291, 322, 362, 364, 454, 461, 562
Bsh1285I	CGRYCG	2 cut(s)	424, 720
BshFI	GGCC	5 cut(s)	65, 163, 208, 737, 808
BshNI	GGYRCC	1 cut(s)	540
BsiEI	CGRYCG	2 cut(s)	424, 720
BsiHKAI	GWGCWC	1 cut(s)	93
BsiHKCI	CYCGRG	1 cut(s)	809
BsiSI	CCGG	7 cut(s)	147, 160, 205, 565, 685, 712, 738
BslFI	GGGAC	3 cut(s)	168, 495, 581
BslI	CCNNNNNNNGG	4 cut(s)	73, 262, 308, 406
BsmFI	GGGAC	3 cut(s)	168, 495, 581
BsnI	GGCC	5 cut(s)	65, 163, 208, 737, 808
BsoBI	CYCGRG	1 cut(s)	809
Bsp120I	GGGCCC	2 cut(s)	63, 806
Bsp1286I	GDGCHC	3 cut(s)	67, 93, 810
Bsp143I	GATC	4 cut(s)	16, 30, 48, 214
Bsp19I	CCATGG	1 cut(s)	129
BspANI	GGCC	5 cut(s)	65, 163, 208, 737, 808
BspCNI	CTCAG	2 cut(s)	264, 597
BspFNI	CGCG	7 cut(s)	291, 322, 362, 364, 454, 461, 562
BspLI	GGNNCC	5 cut(s)	65, 164, 313, 542, 808
BspPI	GGATC	3 cut(s)	11, 25, 43
BspT107I	GGYRCC	1 cut(s)	540
BsrBI	CCGCTC	1 cut(s)	432
BsrFI	RCCGGY	1 cut(s)	737
BsrI	ACTGG	2 cut(s)	180, 576
BssAI	RCCGGY	1 cut(s)	737
BssECI	CCNNGG	5 cut(s)	129, 289, 307, 406, 436
BssMI	GATC	4 cut(s)	16, 30, 48, 214
BssNI	GRCGYC	6 cut(s)	111, 150, 246, 357, 441, 636
BssT1I	CCWWGG	1 cut(s)	129
Bst2UI	CCWGG	4 cut(s)	263, 407, 545, 734
BstACI	GRCGYC	6 cut(s)	111, 150, 246, 357, 441, 636
BstAPI	GCANNNNNTGC	1 cut(s)	649
BstC8I	GCNNGC	1 cut(s)	765
BstDEI	CTNAG	3 cut(s)	251, 605, 700
BstDSI	CCRYGG	3 cut(s)	129, 289, 436
BstENI	CCTNNNNNAGG	1 cut(s)	260
BstF5I	GGATG	3 cut(s)	93, 258, 790
BstFNI	CGCG	7 cut(s)	291, 322, 362, 364, 454, 461, 562
BstH2I	RGCGCY	1 cut(s)	528
BstHHI	GCGC	5 cut(s)	324, 364, 456, 527, 564
BstKTI	GATC	4 cut(s)	19, 33, 51, 217
BstMBI	GATC	4 cut(s)	16, 30, 48, 214
BstMCI	CGRYCG	2 cut(s)	424, 720
BstMWI	GCNNNNNNNGC	5 cut(s)	408, 438, 649, 672, 805
BstNI	CCWGG	4 cut(s)	263, 407, 545, 734
BstNSI	RCATGY	1 cut(s)	767
BstSCI	CCNGG	8 cut(s)	145, 261, 405, 543, 564, 684, 711, 732
BstSFI	CTRYAG	1 cut(s)	220
BstSLI	GKGCMC	2 cut(s)	67, 810
BstUI	CGCG	7 cut(s)	291, 322, 362, 364, 454, 461, 562
BstV2I	GAAGAC	1 cut(s)	148
BstX2I	RGATCY	1 cut(s)	16
BstYI	RGATCY	1 cut(s)	16
BsuRI	GGCC	5 cut(s)	65, 163, 208, 737, 808
BtgI	CCRYGG	3 cut(s)	129, 289, 436
BtsCI	GGATG	3 cut(s)	93, 258, 790
BtsIMutI	CAGTG	1 cut(s)	583
Cac8I	GCNNGC	1 cut(s)	765
CfoI	GCGC	5 cut(s)	324, 364, 456, 527, 564
Cfr10I	RCCGGY	1 cut(s)	737
Cfr13I	GGNCC	8 cut(s)	63, 64, 162, 207, 682, 709, 806, 807
Cfr42I	CCGCGG	1 cut(s)	292
CseI	GACGC	4 cut(s)	119, 139, 254, 430
CsiI	ACCWGGT	1 cut(s)	543
Csp6I	GTAC	4 cut(s)	4, 541, 670, 790
CviAII	CATG	5 cut(s)	130, 646, 759, 764, 800
CviQI	GTAC	4 cut(s)	4, 541, 670, 790
DdeI	CTNAG	3 cut(s)	251, 605, 700
DpnI	GATC	4 cut(s)	18, 32, 50, 216
DpnII	GATC	4 cut(s)	16, 30, 48, 214
DrdI	GACNNNNNNGTC	2 cut(s)	356, 716
DseDI	GACNNNNNNGTC	2 cut(s)	356, 716
EciI	GGCGGA	1 cut(s)	389
Ecl136II	GAGCTC	1 cut(s)	91
Eco130I	CCWWGG	1 cut(s)	129
Eco24I	GRGCYC	3 cut(s)	67, 93, 810
Eco47I	GGWCC	2 cut(s)	682, 709
Eco53kI	GAGCTC	1 cut(s)	91
Eco88I	CYCGRG	1 cut(s)	809
EcoICRI	GAGCTC	1 cut(s)	91
EcoNI	CCTNNNNNAGG	1 cut(s)	260
EcoRI	GAATTC	1 cut(s)	83
EcoRII	CCWGG	4 cut(s)	261, 405, 543, 732
EcoT14I	CCWWGG	1 cut(s)	129
EcoT38I	GRGCYC	3 cut(s)	67, 93, 810
ErhI	CCWWGG	1 cut(s)	129
FaeI	CATG	5 cut(s)	133, 649, 762, 767, 803
FaiI	YATR	7 cut(s)	131, 633, 647, 760, 765, 801, 834
FaqI	GGGAC	3 cut(s)	168, 495, 581
FatI	CATG	5 cut(s)	129, 645, 758, 763, 799
FauI	CCCGC	3 cut(s)	200, 296, 668
FblI	GTMKAC	3 cut(s)	171, 243, 444
Fnu4HI	GCNGC	1 cut(s)	403
FokI	GGATG	3 cut(s)	80, 245, 797
FriOI	GRGCYC	3 cut(s)	67, 93, 810
Fsp4HI	GCNGC	1 cut(s)	403
FspBI	CTAG	1 cut(s)	693
GlaI	GCGC	5 cut(s)	323, 363, 455, 526, 563
GluI	GCNGC	1 cut(s)	403
GsuI	CTGGAG	2 cut(s)	363, 843
HaeII	RGCGCY	1 cut(s)	528
HaeIII	GGCC	5 cut(s)	65, 163, 208, 737, 808
HapII	CCGG	7 cut(s)	147, 160, 205, 565, 685, 712, 738
HgaI	GACGC	4 cut(s)	119, 139, 254, 430
HhaI	GCGC	5 cut(s)	324, 364, 456, 527, 564
Hin1I	GRCGYC	6 cut(s)	111, 150, 246, 357, 441, 636
Hin1II	CATG	5 cut(s)	133, 649, 762, 767, 803
Hin6I	GCGC	5 cut(s)	322, 362, 454, 525, 562
HinP1I	GCGC	5 cut(s)	322, 362, 454, 525, 562
HincII	GTYRAC	4 cut(s)	172, 244, 445, 756
HindII	GTYRAC	4 cut(s)	172, 244, 445, 756
HindIII	AAGCTT	1 cut(s)	664
HpaII	CCGG	7 cut(s)	147, 160, 205, 565, 685, 712, 738
HphI	GGTGA	3 cut(s)	265, 514, 559
Hpy166II	GTNNAC	6 cut(s)	172, 244, 445, 709, 756, 790
Hpy188I	TCNGA	9 cut(s)	21, 30, 82, 214, 283, 597, 606, 620, 745
Hpy188III	TCNNGA	6 cut(s)	46, 74, 380, 480, 721, 772
Hpy8I	GTNNAC	6 cut(s)	172, 244, 445, 709, 756, 790
HpyCH4IV	ACGT	5 cut(s)	102, 240, 357, 515, 636
HpyCH4V	TGCA	3 cut(s)	492, 660, 799
HpyF10VI	GCNNNNNNNGC	5 cut(s)	408, 438, 649, 672, 805
HpyF3I	CTNAG	3 cut(s)	251, 605, 700
HpySE526I	ACGT	5 cut(s)	102, 240, 357, 515, 636
Hsp92I	GRCGYC	6 cut(s)	111, 150, 246, 357, 441, 636
Hsp92II	CATG	5 cut(s)	133, 649, 762, 767, 803
HspAI	GCGC	5 cut(s)	322, 362, 454, 525, 562
KpnI	GGTACC	1 cut(s)	544
KspI	CCGCGG	1 cut(s)	292
Kzo9I	GATC	4 cut(s)	16, 30, 48, 214
LmnI	GCTCC	4 cut(s)	317, 382, 437, 463
LweI	GCATC	2 cut(s)	420, 639
MabI	ACCWGGT	1 cut(s)	543
MaeI	CTAG	1 cut(s)	693
MaeII	ACGT	5 cut(s)	102, 240, 357, 515, 636
MaeIII	GTNAC	2 cut(s)	365, 511
MalI	GATC	4 cut(s)	18, 32, 50, 216
MbiI	CCGCTC	1 cut(s)	432
MboI	GATC	4 cut(s)	16, 30, 48, 214
MboII	GAAGA	3 cut(s)	148, 562, 787
MflI	RGATCY	1 cut(s)	16
MhlI	GDGCHC	3 cut(s)	67, 93, 810
MluCI	AATT	3 cut(s)	83, 590, 600
MlyI	GAGTC	7 cut(s)	19, 264, 278, 377, 440, 477, 491
MmeI	TCCRAC	4 cut(s)	44, 261, 306, 660
MseI	TTAA	1 cut(s)	850
MslI	CAYNNNNRTG	1 cut(s)	128
MspA1I	CMGCKG	1 cut(s)	291
MspI	CCGG	7 cut(s)	147, 160, 205, 565, 685, 712, 738
MspR9I	CCNGG	8 cut(s)	147, 263, 407, 545, 566, 686, 713, 734
MvaI	CCWGG	4 cut(s)	263, 407, 545, 734
MvnI	CGCG	7 cut(s)	291, 322, 362, 364, 454, 461, 562
MwoI	GCNNNNNNNGC	5 cut(s)	408, 438, 649, 672, 805
NciI	CCSGG	4 cut(s)	147, 566, 686, 713
NcoI	CCATGG	1 cut(s)	129
NdeII	GATC	4 cut(s)	16, 30, 48, 214
NlaIII	CATG	5 cut(s)	133, 649, 762, 767, 803
NlaIV	GGNNCC	5 cut(s)	65, 164, 313, 542, 808
NmeAIII	GCCGAG	1 cut(s)	288
NmuCI	GTSAC	2 cut(s)	365, 511
NspI	RCATGY	1 cut(s)	767
PaeI	GCATGC	1 cut(s)	767
PcsI	WCGNNNNNNNCGW	2 cut(s)	354, 492
PfeI	GAWTC	3 cut(s)	77, 226, 553
PflMI	CCANNNNNTGG	1 cut(s)	73
PkrI	GCNGC	1 cut(s)	404
PleI	GAGTC	7 cut(s)	19, 264, 278, 377, 440, 477, 491
PpsI	GAGTC	7 cut(s)	19, 264, 278, 377, 440, 477, 491
Psp124BI	GAGCTC	1 cut(s)	93
Psp6I	CCWGG	4 cut(s)	261, 405, 543, 732
PspGI	CCWGG	4 cut(s)	261, 405, 543, 732
PspN4I	GGNNCC	5 cut(s)	65, 164, 313, 542, 808
PspOMI	GGGCCC	2 cut(s)	63, 806
PspPI	GGNCC	8 cut(s)	63, 64, 162, 207, 682, 709, 806, 807
PsuI	RGATCY	1 cut(s)	16
RsaI	GTAC	4 cut(s)	5, 542, 671, 791
RsaNI	GTAC	4 cut(s)	4, 541, 670, 790
RseI	CAYNNNNRTG	1 cut(s)	128
SacI	GAGCTC	1 cut(s)	93
SacII	CCGCGG	1 cut(s)	292
SalI	GTCGAC	3 cut(s)	170, 242, 443
SaqAI	TTAA	1 cut(s)	850
SatI	GCNGC	1 cut(s)	403
Sau3AI	GATC	4 cut(s)	16, 30, 48, 214
Sau96I	GGNCC	8 cut(s)	63, 64, 162, 207, 682, 709, 806, 807
SchI	GAGTC	7 cut(s)	19, 264, 278, 377, 440, 477, 491
ScrFI	CCNGG	8 cut(s)	147, 263, 407, 545, 566, 686, 713, 734
SduI	GDGCHC	3 cut(s)	67, 93, 810
SexAI	ACCWGGT	1 cut(s)	543
SfaNI	GCATC	2 cut(s)	420, 639
SfcI	CTRYAG	1 cut(s)	220
Sfr303I	CCGCGG	1 cut(s)	292
SgrBI	CCGCGG	1 cut(s)	292
SgrDI	CGTCGACG	1 cut(s)	242
SinI	GGWCC	2 cut(s)	682, 709
SmiMI	CAYNNNNRTG	1 cut(s)	128
SphI	GCATGC	1 cut(s)	767
Sse9I	AATT	3 cut(s)	83, 590, 600
SspMI	CTAG	1 cut(s)	693
SstI	GAGCTC	1 cut(s)	93
StyD4I	CCNGG	8 cut(s)	145, 261, 405, 543, 564, 684, 711, 732
StyI	CCWWGG	1 cut(s)	129
TaiI	ACGT	5 cut(s)	105, 243, 360, 518, 639
TaqI	TCGA	9 cut(s)	87, 108, 171, 243, 354, 390, 414, 420, 444
TasI	AATT	3 cut(s)	83, 590, 600
TatI	WGTACW	1 cut(s)	789
TauI	GCSGC	1 cut(s)	405
TfiI	GAWTC	3 cut(s)	77, 226, 553
Tru1I	TTAA	1 cut(s)	850
Tru9I	TTAA	1 cut(s)	850
TscAI	CASTG	1 cut(s)	583
TseFI	GTSAC	2 cut(s)	365, 511
Tsp45I	GTSAC	2 cut(s)	365, 511
TspDTI	ATGAA	2 cut(s)	112, 316
TspGWI	ACGGA	1 cut(s)	41
TspRI	CASTG	1 cut(s)	583
Van91I	CCANNNNNTGG	1 cut(s)	73
VpaK11BI	GGWCC	2 cut(s)	682, 709
XagI	CCTNNNNNAGG	1 cut(s)	260
XapI	RAATTY	3 cut(s)	83, 590, 600
XceI	RCATGY	1 cut(s)	767
XmiI	GTMKAC	3 cut(s)	171, 243, 444
XspI	CTAG	1 cut(s)	693
ZraI	GACGTC	2 cut(s)	358, 637

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.