AT5G25560

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005253
Physical Location & Seq
Forward (+)
8897797 .. 8901740
3944 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25560.1

Sequence Viewer

Length: 927 bp
ATGGGAGACGTATATTTCAACCATTTCGCTCAGCAACTGCAGATGTCTGACCAAGAGAAGGGTGAAATGTCAAGACATTCACACCCTCATTCAATCAATGAAGAATCTGAGTCGTCAACTCTGGAAAGGGTAGCAGCAGAGTCATTAACAAACAAAGTCCTTGATAGAGGACTAATGGAGTATGGATGTCCACATTACAGAAGAAGGTGTTGCATTAGAGCTCCTTGCTGTAATGAGATCTTTGGTTGCCATCACTGTCATTATGAGGCGAAGAACAACATAAATGTAGATCAGAAGCAGAGGCATGATATTCCTCGCCACCAAGTTGAACAGGTTATTTGTTTACTTTGTGGTACTGAACAAGAGGTGGGCCAAATTTGTATCCACTGTGGAGTGTGCATGGGGAAGTACTTCTGTAAAGTCTGCAAGCTCTACGATGATGATACGTCTAAAAAACAGTACCACTGCGATGGGTGTGGAATTTGCAGAATCGGAGGACGTGAAAATTTCTTTCACTGTTACAAGTGTGGCTGTTGTTATTCAATCCTCCTCAAGAACGGTCACCCTTGTGTCGAAGGAGCAATGCATCACGACTGCCCCATTTGCTTTGAGTTTCTGTTTGAATCACGGAACGATGTGACGGTTTTACCATGTGGACACACAATTCATCAGAAGTGCTTGGAGGAAATGAGAGATCATTACCAATATGCGTGCCCACTTTGCTCAAAATCTGTATGCGACATGTCAAAAGTGTGGGAGAAGTTTGACATGGAGATTGCGGCTACACCAATGCCAGAACCATACCAGAACAGAATGGTTCAGATTCTCTGCAACGACTGTGGGAAGAAAGCAGAGGTACAGTACCATGTGGTGGCTCAGAAATGCCCAAACTGCAAATCATACAACACTCGCCAAACCAGAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0002039 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0008270 GO:0009056 GO:0009057 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0032991 GO:0036211 GO:0042176 GO:0042802 GO:0042803 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0046872 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051865 GO:0060255 GO:0061136 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1902494 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:1990234 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
ko04115 ko04120 ko05162 map04115 map04120 map05162
Pfam Domains
Protein Families
zf-CHY zf-RING_2 zinc_ribbon_6

Protein Analysis

308

Amino Acids

35.51

Weight (kDa)

6.68

Isoelectric Point (pI)

50.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CHY PF05495 63 - 137 1.1e-16 CHY zinc finger
zf-RING_2 PF13639 198 - 241 4.5e-09 Ring finger domain
zf-RING_5 PF14634 198 - 243 1.2e-07 zinc-RING finger domain
zf-RING_UBOX PF13445 199 - 239 1.6e-07 RING-type zinc-finger
zf-C3HC4 PF00097 199 - 241 7.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 199 - 241 2.3e-06 Zinc finger, C3HC4 type (RING finger)
zinc_ribbon_6 PF14599 246 - 304 6.3e-27 Zinc-ribbon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 871
AciI CCGC 1 cut(s) 779
AcsI RAATTY 3 cut(s) 375, 480, 505
AfaI GTAC 5 cut(s) 355, 410, 461, 858, 863
AfiI CCNNNNNNNGG 2 cut(s) 58, 871
AflIII ACRYGT 1 cut(s) 741
AgsI TTSAA 5 cut(s) 19, 93, 329, 543, 623
AjiI CACGTC 1 cut(s) 500
AleI CACNNNNGTG 1 cut(s) 567
AluBI AGCT 2 cut(s) 221, 430
AluI AGCT 2 cut(s) 221, 430
Alw21I GWGCWC 1 cut(s) 223
AlwNI CAGNNNCTG 2 cut(s) 37, 924
AoxI GGCC 1 cut(s) 370
ApeKI GCWGC 1 cut(s) 134
ApoI RAATTY 3 cut(s) 375, 480, 505
Asp700I GAANNNNTTC 1 cut(s) 410
AspS9I GGNCC 1 cut(s) 370
AsuHPI GGTGA 2 cut(s) 74, 554
BaeGI GKGCMC 1 cut(s) 716
BanII GRGCYC 1 cut(s) 223
Bbv12I GWGCWC 1 cut(s) 223
BbvI GCAGC 1 cut(s) 146
BccI CCATC 2 cut(s) 258, 464
BciVI GTATCC 1 cut(s) 392
BfmI CTRYAG 1 cut(s) 38
BfuI GTATCC 1 cut(s) 392
BglII AGATCT 1 cut(s) 237
BisI GCNGC 2 cut(s) 135, 780
BlpI GCTNAGC 1 cut(s) 30
BlsI GCNGC 2 cut(s) 136, 781
BmcAI AGTACT 1 cut(s) 410
BmgBI CACGTC 1 cut(s) 500
BmgT120I GGNCC 1 cut(s) 370
BmsI GCATC 1 cut(s) 595
Bpu1102I GCTNAGC 1 cut(s) 30
BpuEI CTTGAG 1 cut(s) 536
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 871
Bse3DI GCAATG 1 cut(s) 588
BseGI GGATG 1 cut(s) 191
BseLI CCNNNNNNNGG 2 cut(s) 58, 871
BseMI GCAATG 1 cut(s) 588
BseMII CTCAG 3 cut(s) 44, 99, 890
BseRI GAGGAG 1 cut(s) 539
BseSI GKGCMC 1 cut(s) 716
BseXI GCAGC 1 cut(s) 146
BshFI GGCC 1 cut(s) 372
BsiHKAI GWGCWC 1 cut(s) 223
BslI CCNNNNNNNGG 2 cut(s) 58, 871
BsnI GGCC 1 cut(s) 372
Bsp1286I GDGCHC 2 cut(s) 223, 716
Bsp143I GATC 3 cut(s) 237, 289, 694
Bsp1720I GCTNAGC 1 cut(s) 30
BspACI CCGC 1 cut(s) 779
BspANI GGCC 1 cut(s) 372
BspCNI CTCAG 3 cut(s) 43, 100, 889
BspMAI CTGCAG 1 cut(s) 42
BsrDI GCAATG 1 cut(s) 588
BssMI GATC 3 cut(s) 237, 289, 694
Bst4CI ACNGT 8 cut(s) 257, 389, 459, 518, 560, 643, 839, 861
BstC8I GCNNGC 2 cut(s) 428, 712
BstDEI CTNAG 3 cut(s) 30, 108, 876
BstEII GGTNACC 1 cut(s) 560
BstF5I GGATG 1 cut(s) 191
BstKTI GATC 3 cut(s) 240, 292, 697
BstMBI GATC 3 cut(s) 237, 289, 694
BstMWI GCNNNNNNNGC 3 cut(s) 603, 720, 891
BstNSI RCATGY 1 cut(s) 745
BstPI GGTNACC 1 cut(s) 560
BstSFI CTRYAG 1 cut(s) 38
BstSLI GKGCMC 1 cut(s) 716
BstV1I GCAGC 1 cut(s) 146
BstX2I RGATCY 1 cut(s) 237
BstXI CCANNNNNNTGG 1 cut(s) 470
BstYI RGATCY 1 cut(s) 237
BsuI GTATCC 1 cut(s) 392
BsuRI GGCC 1 cut(s) 372
BtgZI GCGATG 1 cut(s) 483
BtrI CACGTC 1 cut(s) 500
BtsCI GGATG 1 cut(s) 191
BtsI GCAGTG 1 cut(s) 463
BtsIMutI CAGTG 4 cut(s) 253, 385, 463, 514
Cac8I GCNNGC 2 cut(s) 428, 712
CaiI CAGNNNCTG 2 cut(s) 37, 924
Cfr13I GGNCC 1 cut(s) 370
Csp6I GTAC 5 cut(s) 354, 409, 460, 857, 862
CspCI CAANNNNNGTGG 2 cut(s) 820, 855
CviAII CATG 6 cut(s) 305, 400, 651, 742, 769, 866
CviJI RGCY 7 cut(s) 221, 372, 430, 531, 782, 875, 924
CviKI_1 RGCY 7 cut(s) 221, 372, 430, 531, 782, 875, 924
CviQI GTAC 5 cut(s) 354, 409, 460, 857, 862
DdeI CTNAG 3 cut(s) 30, 108, 876
DpnI GATC 3 cut(s) 239, 291, 696
DpnII GATC 3 cut(s) 237, 289, 694
Ecl136II GAGCTC 1 cut(s) 221
Eco24I GRGCYC 1 cut(s) 223
Eco53kI GAGCTC 1 cut(s) 221
Eco91I GGTNACC 1 cut(s) 560
EcoICRI GAGCTC 1 cut(s) 221
EcoO65I GGTNACC 1 cut(s) 560
EcoT22I ATGCAT 1 cut(s) 588
EcoT38I GRGCYC 1 cut(s) 223
FaeI CATG 6 cut(s) 308, 403, 654, 745, 772, 869
FatI CATG 6 cut(s) 304, 399, 650, 741, 768, 865
Fnu4HI GCNGC 2 cut(s) 135, 780
FokI GGATG 1 cut(s) 198
FriOI GRGCYC 1 cut(s) 223
Fsp4HI GCNGC 2 cut(s) 135, 780
GluI GCNGC 2 cut(s) 135, 780
HaeIII GGCC 1 cut(s) 372
Hin1II CATG 6 cut(s) 308, 403, 654, 745, 772, 869
HincII GTYRAC 1 cut(s) 117
HindII GTYRAC 1 cut(s) 117
HinfI GANTC 6 cut(s) 104, 110, 140, 489, 623, 823
HphI GGTGA 2 cut(s) 74, 554
Hpy166II GTNNAC 4 cut(s) 117, 191, 344, 656
Hpy188I TCNGA 7 cut(s) 49, 109, 294, 494, 672, 822, 879
Hpy188III TCNNGA 4 cut(s) 72, 122, 553, 590
Hpy8I GTNNAC 4 cut(s) 117, 191, 344, 656
HpyAV CCTTC 3 cut(s) 52, 198, 569
HpyCH4III ACNGT 8 cut(s) 257, 389, 459, 518, 560, 643, 839, 861
HpyCH4IV ACGT 3 cut(s) 9, 446, 499
HpyCH4V TGCA 8 cut(s) 40, 213, 399, 426, 486, 586, 831, 894
HpyF10VI GCNNNNNNNGC 3 cut(s) 603, 720, 891
HpyF3I CTNAG 3 cut(s) 30, 108, 876
HpySE526I ACGT 3 cut(s) 9, 446, 499
Hsp92II CATG 6 cut(s) 308, 403, 654, 745, 772, 869
Kzo9I GATC 3 cut(s) 237, 289, 694
LmnI GCTCC 2 cut(s) 226, 578
LpnPI CCDG 4 cut(s) 107, 317, 807, 818
Lsp1109I GCAGC 1 cut(s) 146
LweI GCATC 1 cut(s) 595
MaeII ACGT 3 cut(s) 9, 446, 499
MaeIII GTNAC 3 cut(s) 518, 560, 637
MalI GATC 3 cut(s) 239, 291, 696
MboI GATC 3 cut(s) 237, 289, 694
MboII GAAGA 4 cut(s) 113, 213, 283, 856
MflI RGATCY 1 cut(s) 237
MhlI GDGCHC 2 cut(s) 223, 716
MluCI AATT 4 cut(s) 375, 480, 505, 663
MlyI GAGTC 2 cut(s) 119, 149
Mph1103I ATGCAT 1 cut(s) 588
MroXI GAANNNNTTC 1 cut(s) 410
MseI TTAA 1 cut(s) 146
MslI CAYNNNNRTG 2 cut(s) 468, 567
MwoI GCNNNNNNNGC 3 cut(s) 603, 720, 891
NdeII GATC 3 cut(s) 237, 289, 694
NlaIII CATG 6 cut(s) 308, 403, 654, 745, 772, 869
NmuCI GTSAC 2 cut(s) 560, 637
NsiI ATGCAT 1 cut(s) 588
NspI RCATGY 1 cut(s) 745
OliI CACNNNNGTG 1 cut(s) 567
PciI ACATGT 1 cut(s) 741
PdmI GAANNNNTTC 1 cut(s) 410
PfeI GAWTC 4 cut(s) 104, 489, 623, 823
PflMI CCANNNNNTGG 1 cut(s) 871
PkrI GCNGC 2 cut(s) 136, 781
PleI GAGTC 2 cut(s) 118, 148
PpsI GAGTC 2 cut(s) 118, 148
PscI ACATGT 1 cut(s) 741
Psp124BI GAGCTC 1 cut(s) 223
PspEI GGTNACC 1 cut(s) 560
PspPI GGNCC 1 cut(s) 370
PstI CTGCAG 1 cut(s) 42
PstNI CAGNNNCTG 2 cut(s) 37, 924
PsuI RGATCY 1 cut(s) 237
RsaI GTAC 5 cut(s) 355, 410, 461, 858, 863
RsaNI GTAC 5 cut(s) 354, 409, 460, 857, 862
RseI CAYNNNNRTG 2 cut(s) 468, 567
SacI GAGCTC 1 cut(s) 223
SaqAI TTAA 1 cut(s) 146
SatI GCNGC 2 cut(s) 135, 780
Sau3AI GATC 3 cut(s) 237, 289, 694
Sau96I GGNCC 1 cut(s) 370
ScaI AGTACT 1 cut(s) 410
SchI GAGTC 2 cut(s) 119, 149
SduI GDGCHC 2 cut(s) 223, 716
SetI ASST 9 cut(s) 12, 209, 223, 336, 369, 432, 449, 502, 858
SfaNI GCATC 1 cut(s) 595
SfcI CTRYAG 1 cut(s) 38
SmiMI CAYNNNNRTG 2 cut(s) 468, 567
SmlI CTYRAG 1 cut(s) 551
SmoI CTYRAG 1 cut(s) 551
Sse9I AATT 4 cut(s) 375, 480, 505, 663
SsiI CCGC 1 cut(s) 779
SstI GAGCTC 1 cut(s) 223
TaaI ACNGT 8 cut(s) 257, 389, 459, 518, 560, 643, 839, 861
TaiI ACGT 3 cut(s) 12, 449, 502
TaqI TCGA 1 cut(s) 573
TasI AATT 4 cut(s) 375, 480, 505, 663
TatI WGTACW 1 cut(s) 408
TauI GCSGC 1 cut(s) 782
TfiI GAWTC 4 cut(s) 104, 489, 623, 823
Tru1I TTAA 1 cut(s) 146
Tru9I TTAA 1 cut(s) 146
TscAI CASTG 4 cut(s) 260, 392, 470, 521
TseFI GTSAC 2 cut(s) 560, 637
TseI GCWGC 1 cut(s) 134
Tsp45I GTSAC 2 cut(s) 560, 637
TspDTI ATGAA 2 cut(s) 114, 656
TspGWI ACGGA 1 cut(s) 643
TspRI CASTG 4 cut(s) 260, 392, 470, 521
Van91I CCANNNNNTGG 1 cut(s) 871
XapI RAATTY 3 cut(s) 375, 480, 505
XceI RCATGY 1 cut(s) 745
XmnI GAANNNNTTC 1 cut(s) 410
ZrmI AGTACT 1 cut(s) 410
Zsp2I ATGCAT 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.