MD08G1135100.v1.1

RING finger and CHY zinc finger domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0005253
Physical Location & Seq
Reverse (-)
12835125 .. 12840184
5060 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1135100.v1.1.491

Sequence Viewer

Length: 882 bp
ATGCAAGATATAATGGTTAGGCAGAACATAATGGGGGATGCCCACTGTAATCTATCAAATGGCGAGTATAAGCTCAAACTTGAATCGAAGGTTACTGGGGAGGCCACTGAATTGCTAGAGAAAGGATACATGCAGTATGGATGTCCACACTATCGGCGAAGGTGCAGTATTAGAGCTCCTTGCTGCAATGAGATTTTTGATTGTCGCCATTGTCATAATGAAGCTAAGAATGATATTAGTGTTGATCAGAAGCATAGACATGACATGCCGCGTCATCAAGTGAAACAGGTGATATGTTCACTTTGCGACACTGAGCAAGAGGTTCAACAAATTTGTGTCAACTGTGGTGTATGTATGGGGAAATACTTCTGTGAGACTTGCAAGCTATTTGATGATGATGTATCTAAGAAACAGTACCATTGCAATGGCTGTGGGATTTGCAGAATTGGGGGGCGCGAGAATTTCTTTCACTGCTACAAGTGTGGTTGCTGCTACTCAGTGCTGTTGAAGAACAGCCACCCCTGTGTGGAGGGAGCAATGCATCACGACTGCCCTGTTTGCTTTGAGTTCTTATTCGAAACAAGAAATGATGTTACCGTCATGCCATGTGGGCACACAATTCACAAGAATTGCTTAAAAGAGATGAGGGATCATTATCAATATGCTTGCCCTCTTTGCTCCAAGTCAGTTTGCGATATGTCTAAGGTATGGGAGAAATATGACATAGAGATTGCAGCTCAACCGATGCCTGAACCCTATCAAAACAGATTGATTTCGATCCTCTGCAACGACTGCGGGAAAAGCTCACAGGTGCAGTACCATGTTGTAGCCCGGAAATGTCTGAACTGTAAATCTTACAACACTCGCCAAACGAGAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0002039 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0008270 GO:0009056 GO:0009057 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0032991 GO:0036211 GO:0042176 GO:0042802 GO:0042803 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0046872 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051865 GO:0060255 GO:0061136 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1902494 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:1990234 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
ko04115 ko04120 ko05162 map04115 map04120 map05162
Pfam Domains
Protein Families
zf-CHY zf-RING_2 zinc_ribbon_6

Protein Analysis

294

Amino Acids

33.84

Weight (kDa)

7.84

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CHY PF05495 48 - 122 5.1e-19 CHY zinc finger
zf-RING_2 PF13639 183 - 226 2.9e-08 Ring finger domain
zf-RING_5 PF14634 183 - 228 3.5e-07 zinc-RING finger domain
zf-RING_UBOX PF13445 184 - 224 4.7e-07 RING-type zinc-finger
zf-C3HC4 PF00097 184 - 226 1.3e-06 Zinc finger, C3HC4 type (RING finger)
zinc_ribbon_6 PF14599 231 - 289 3.1e-25 Zinc-ribbon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 271, 456
AciI CCGC 2 cut(s) 269, 795
AclWI GGATC 2 cut(s) 657, 772
AcsI RAATTY 2 cut(s) 330, 460
AfaI GTAC 2 cut(s) 416, 818
AfiI CCNNNNNNNGG 1 cut(s) 526
AgsI TTSAA 3 cut(s) 83, 326, 508
AleI CACNNNNGTG 1 cut(s) 522
AluBI AGCT 6 cut(s) 73, 176, 224, 385, 737, 804
AluI AGCT 6 cut(s) 73, 176, 224, 385, 737, 804
Alw21I GWGCWC 1 cut(s) 178
Alw26I GTCTC 1 cut(s) 368
AlwI GGATC 2 cut(s) 657, 772
AoxI GGCC 1 cut(s) 102
ApeKI GCWGC 3 cut(s) 183, 489, 734
ApoI RAATTY 2 cut(s) 330, 460
Asp700I GAANNNNTTC 1 cut(s) 365
AspLEI GCGC 1 cut(s) 456
AsuC2I CCSGG 1 cut(s) 832
AsuHPI GGTGA 1 cut(s) 301
AsuII TTCGAA 1 cut(s) 576
BaeGI GKGCMC 1 cut(s) 615
BanII GRGCYC 1 cut(s) 178
Bbv12I GWGCWC 1 cut(s) 178
BbvI GCAGC 3 cut(s) 170, 476, 746
BciVI GTATCC 1 cut(s) 119
BclI TGATCA 1 cut(s) 244
BcnI CCSGG 1 cut(s) 832
BcoDI GTCTC 1 cut(s) 368
BfaI CTAG 1 cut(s) 116
BfuI GTATCC 1 cut(s) 119
BglI GCCNNNNNGGC 1 cut(s) 610
BisI GCNGC 4 cut(s) 184, 269, 490, 735
BlsI GCNGC 4 cut(s) 185, 270, 491, 736
Bme1390I CCNGG 1 cut(s) 832
BmrFI CCNGG 1 cut(s) 832
BmrI ACTGGG 1 cut(s) 105
BmsI GCATC 3 cut(s) 28, 550, 735
BmuI ACTGGG 1 cut(s) 105
Bpu14I TTCGAA 1 cut(s) 576
BpuMI CCSGG 1 cut(s) 832
BsaBI GATNNNNATC 2 cut(s) 654, 776
Bsc4I CCNNNNNNNGG 1 cut(s) 526
Bse1I ACTGG 1 cut(s) 100
Bse3DI GCAATG 4 cut(s) 193, 418, 430, 543
Bse8I GATNNNNATC 2 cut(s) 654, 776
BseGI GGATG 2 cut(s) 43, 146
BseJI GATNNNNATC 2 cut(s) 654, 776
BseLI CCNNNNNNNGG 1 cut(s) 526
BseMI GCAATG 4 cut(s) 193, 418, 430, 543
BseMII CTCAG 2 cut(s) 303, 510
BseNI ACTGG 1 cut(s) 100
BseSI GKGCMC 1 cut(s) 615
BseXI GCAGC 3 cut(s) 170, 476, 746
BsgI GTGCAG 2 cut(s) 184, 833
Bsh1236I CGCG 2 cut(s) 271, 456
BshFI GGCC 1 cut(s) 104
BsiHKAI GWGCWC 1 cut(s) 178
BsiSI CCGG 1 cut(s) 832
BslI CCNNNNNNNGG 1 cut(s) 526
BsmAI GTCTC 1 cut(s) 368
BsnI GGCC 1 cut(s) 104
Bsp119I TTCGAA 1 cut(s) 576
Bsp1286I GDGCHC 2 cut(s) 178, 615
Bsp143I GATC 3 cut(s) 244, 649, 777
BspACI CCGC 2 cut(s) 269, 795
BspANI GGCC 1 cut(s) 104
BspCNI CTCAG 2 cut(s) 304, 509
BspFNI CGCG 2 cut(s) 271, 456
BspPI GGATC 2 cut(s) 657, 772
BspT104I TTCGAA 1 cut(s) 576
BsrDI GCAATG 4 cut(s) 193, 418, 430, 543
BsrI ACTGG 1 cut(s) 100
BssMI GATC 3 cut(s) 244, 649, 777
Bst4CI ACNGT 5 cut(s) 47, 344, 414, 598, 848
BstAPI GCANNNNNTGC 1 cut(s) 792
BstBI TTCGAA 1 cut(s) 576
BstC8I GCNNGC 2 cut(s) 383, 667
BstDEI CTNAG 5 cut(s) 225, 312, 405, 496, 702
BstF5I GGATG 2 cut(s) 43, 146
BstFNI CGCG 2 cut(s) 271, 456
BstHHI GCGC 1 cut(s) 456
BstKTI GATC 3 cut(s) 247, 652, 780
BstMAI GTCTC 1 cut(s) 368
BstMBI GATC 3 cut(s) 244, 649, 777
BstMWI GCNNNNNNNGC 5 cut(s) 558, 610, 675, 792, 801
BstNSI RCATGY 2 cut(s) 133, 268
BstSCI CCNGG 1 cut(s) 830
BstSLI GKGCMC 1 cut(s) 615
BstUI CGCG 2 cut(s) 271, 456
BstV1I GCAGC 3 cut(s) 170, 476, 746
BstXI CCANNNNNNTGG 1 cut(s) 425
BsuI GTATCC 1 cut(s) 119
BsuRI GGCC 1 cut(s) 104
BtsCI GGATG 2 cut(s) 43, 146
BtsI GCAGTG 1 cut(s) 469
BtsIMutI CAGTG 5 cut(s) 43, 105, 309, 469, 504
Cac8I GCNNGC 2 cut(s) 383, 667
CfoI GCGC 1 cut(s) 456
CseI GACGC 1 cut(s) 260
Csp6I GTAC 2 cut(s) 415, 817
CspCI CAANNNNNGTGG 2 cut(s) 412, 447
CviAII CATG 6 cut(s) 130, 260, 265, 601, 606, 821
CviQI GTAC 2 cut(s) 415, 817
DdeI CTNAG 5 cut(s) 225, 312, 405, 496, 702
DpnI GATC 3 cut(s) 246, 651, 779
DpnII GATC 3 cut(s) 244, 649, 777
Ecl136II GAGCTC 1 cut(s) 176
Eco24I GRGCYC 1 cut(s) 178
Eco53kI GAGCTC 1 cut(s) 176
EcoICRI GAGCTC 1 cut(s) 176
EcoT22I ATGCAT 1 cut(s) 543
EcoT38I GRGCYC 1 cut(s) 178
FaeI CATG 6 cut(s) 133, 263, 268, 604, 609, 824
FalI AAGNNNNNCTT 2 cut(s) 617, 649
FatI CATG 6 cut(s) 129, 259, 264, 600, 605, 820
FauI CCCGC 1 cut(s) 788
FbaI TGATCA 1 cut(s) 244
Fnu4HI GCNGC 4 cut(s) 184, 269, 490, 735
FokI GGATG 2 cut(s) 50, 153
FriOI GRGCYC 1 cut(s) 178
Fsp4HI GCNGC 4 cut(s) 184, 269, 490, 735
FspBI CTAG 1 cut(s) 116
GlaI GCGC 1 cut(s) 455
GluI GCNGC 4 cut(s) 184, 269, 490, 735
HaeIII GGCC 1 cut(s) 104
HapII CCGG 1 cut(s) 832
HgaI GACGC 1 cut(s) 260
HhaI GCGC 1 cut(s) 456
Hin1II CATG 6 cut(s) 133, 263, 268, 604, 609, 824
Hin6I GCGC 1 cut(s) 454
HinP1I GCGC 1 cut(s) 454
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 1 cut(s) 83
HpaII CCGG 1 cut(s) 832
HphI GGTGA 1 cut(s) 301
Hpy166II GTNNAC 3 cut(s) 146, 299, 340
Hpy188I TCNGA 2 cut(s) 249, 843
Hpy188III TCNNGA 1 cut(s) 545
Hpy8I GTNNAC 3 cut(s) 146, 299, 340
HpyAV CCTTC 2 cut(s) 82, 153
HpyCH4III ACNGT 5 cut(s) 47, 344, 414, 598, 848
HpyF10VI GCNNNNNNNGC 5 cut(s) 558, 610, 675, 792, 801
HpyF3I CTNAG 5 cut(s) 225, 312, 405, 496, 702
Hsp92II CATG 6 cut(s) 133, 263, 268, 604, 609, 824
HspAI GCGC 1 cut(s) 454
Ksp22I TGATCA 1 cut(s) 244
Kzo9I GATC 3 cut(s) 244, 649, 777
LmnI GCTCC 3 cut(s) 181, 533, 683
LpnPI CCDG 7 cut(s) 81, 272, 535, 567, 762, 794, 845
Lsp1109I GCAGC 3 cut(s) 170, 476, 746
LweI GCATC 3 cut(s) 28, 550, 735
MaeI CTAG 1 cut(s) 116
MaeIII GTNAC 2 cut(s) 91, 592
MalI GATC 3 cut(s) 246, 651, 779
MboI GATC 3 cut(s) 244, 649, 777
MboII GAAGA 1 cut(s) 520
MhlI GDGCHC 2 cut(s) 178, 615
MluCI AATT 6 cut(s) 110, 330, 444, 460, 618, 628
MnlI CCTC 7 cut(s) 94, 313, 523, 639, 681, 791, 869
Mph1103I ATGCAT 1 cut(s) 543
MroXI GAANNNNTTC 1 cut(s) 365
MseI TTAA 1 cut(s) 635
MslI CAYNNNNRTG 3 cut(s) 258, 423, 522
MspI CCGG 1 cut(s) 832
MspR9I CCNGG 1 cut(s) 832
MvnI CGCG 2 cut(s) 271, 456
MwoI GCNNNNNNNGC 5 cut(s) 558, 610, 675, 792, 801
NciI CCSGG 1 cut(s) 832
NdeII GATC 3 cut(s) 244, 649, 777
NlaIII CATG 6 cut(s) 133, 263, 268, 604, 609, 824
NsiI ATGCAT 1 cut(s) 543
NspI RCATGY 2 cut(s) 133, 268
NspV TTCGAA 1 cut(s) 576
OliI CACNNNNGTG 1 cut(s) 522
PdmI GAANNNNTTC 1 cut(s) 365
PfeI GAWTC 1 cut(s) 83
PkrI GCNGC 4 cut(s) 185, 270, 491, 736
Psp124BI GAGCTC 1 cut(s) 178
RsaI GTAC 2 cut(s) 416, 818
RsaNI GTAC 2 cut(s) 415, 817
RseI CAYNNNNRTG 3 cut(s) 258, 423, 522
SacI GAGCTC 1 cut(s) 178
SaqAI TTAA 1 cut(s) 635
SatI GCNGC 4 cut(s) 184, 269, 490, 735
Sau3AI GATC 3 cut(s) 244, 649, 777
ScrFI CCNGG 1 cut(s) 832
SduI GDGCHC 2 cut(s) 178, 615
SfaNI GCATC 3 cut(s) 28, 550, 735
SfuI TTCGAA 1 cut(s) 576
SmiMI CAYNNNNRTG 3 cut(s) 258, 423, 522
Sse9I AATT 6 cut(s) 110, 330, 444, 460, 618, 628
SsiI CCGC 2 cut(s) 269, 795
SspMI CTAG 1 cut(s) 116
SstI GAGCTC 1 cut(s) 178
StyD4I CCNGG 1 cut(s) 830
TaaI ACNGT 5 cut(s) 47, 344, 414, 598, 848
TaqI TCGA 3 cut(s) 86, 576, 776
TasI AATT 6 cut(s) 110, 330, 444, 460, 618, 628
TauI GCSGC 1 cut(s) 271
TfiI GAWTC 1 cut(s) 83
Tru1I TTAA 1 cut(s) 635
Tru9I TTAA 1 cut(s) 635
TscAI CASTG 5 cut(s) 50, 112, 316, 476, 504
TseI GCWGC 3 cut(s) 183, 489, 734
TspDTI ATGAA 1 cut(s) 234
TspRI CASTG 5 cut(s) 50, 112, 316, 476, 504
XapI RAATTY 2 cut(s) 330, 460
XceI RCATGY 2 cut(s) 133, 268
XmnI GAANNNNTTC 1 cut(s) 365
XspI CTAG 1 cut(s) 116
Zsp2I ATGCAT 1 cut(s) 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.