FvH4_2g30250

RING finger and CHY zinc finger domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005253
Physical Location & Seq
Forward (+)
23388654 .. 23393052
4399 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g30250.t3

Sequence Viewer

Length: 867 bp
ATGGCCACAGAGATAGTGATGGAGAATTTCCACTCTTATCCATCAAATGGGAAGTGCATGGTCACAATAGAGGCAAAGGCCAATGAAAAGTCCAAGTCTTTAGACAGAGGACTTGGAGACTTCGGATGTTCACATTATAGACGAAGGTGCCGCATTAGGGCTCCTTGTTGCAATGAAATTGTTGATTGTCGCCATTGCCATAATGAAGCAAAGAATAATATTAATGTTGATCCAAAGCTTAGACATGACATACCGCGCCATCAAGTCAAACATGTGATATGTTCACTTTGCAACACCGAGCAAGAGGTTCAACAAGTCTGTGTCAACTGTGGTGTATGTATGGGCAAATATTTCTGTGAGACATGCAAGCTATTTGATGACGATATATCCAAGAGACAGTACCATTGCCATGGATGTGGAATCTGCAGAATTGGGGGAGCTGATAATTTCTTTCACTGCAATAAATGTGGCTGCTGCTACTCAATTCTGCTGAAAAACAGTCACCCTTGTGTGGAAGGAGCAATGCATCATGACTGCCCTGTTTGCTTTGAGTTCTTATTCGACTCAAGAAATGATGTTACGGTGATGCCGTGTGGACATACCATTCACAAGCATTGTCTGAAAGAGATGAGAGACCATATGCAGTACGCATGCCCTCTTTGCTCCAAGTCAGTCTGTGATATGTCCAAGGTATGGGAGAAATACGACATGGAGATCGCAGCTACGCCGATGCCCGAACCATATCAGAATAAATTGGTTCCAATTTTGTGCAATGATTGCGGGAAGAGCTCAGAAGTGCAGTTCCATATTGTGGCTCAGAAATGCTCAAGCTGCAAATCTTACAACACTCGCCAAACAAGAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0002039 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0008270 GO:0009056 GO:0009057 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0032991 GO:0036211 GO:0042176 GO:0042802 GO:0042803 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0046872 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051865 GO:0060255 GO:0061136 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1902494 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:1990234 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
ko04115 ko04120 ko05162 map04115 map04120 map05162
Pfam Domains
Protein Families
zf-CHY zf-RING_2 zinc_ribbon_6

Protein Analysis

289

Amino Acids

32.84

Weight (kDa)

8.0

Isoelectric Point (pI)

49.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CHY PF05495 43 - 117 2e-17 CHY zinc finger
zf-RING_2 PF13639 178 - 221 1.3e-08 Ring finger domain
zf-RING_5 PF14634 178 - 223 3.9e-07 zinc-RING finger domain
zf-RING_UBOX PF13445 179 - 219 1.1e-06 RING-type zinc-finger
zf-C3HC4 PF00097 179 - 221 2.6e-06 Zinc finger, C3HC4 type (RING finger)
zinc_ribbon_6 PF14599 226 - 284 3.8e-26 Zinc-ribbon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 147
AccB7I CCANNNNNTGG 3 cut(s) 47, 693, 811
AccII CGCG 1 cut(s) 256
AciI CCGC 3 cut(s) 151, 254, 780
AclWI GGATC 1 cut(s) 224
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 25
AfaI GTAC 2 cut(s) 401, 647
AfiI CCNNNNNNNGG 5 cut(s) 47, 157, 511, 693, 811
AflIII ACRYGT 1 cut(s) 271
AgsI TTSAA 1 cut(s) 311
AleI CACNNNNGTG 1 cut(s) 507
AluBI AGCT 6 cut(s) 238, 370, 440, 722, 789, 831
AluI AGCT 6 cut(s) 238, 370, 440, 722, 789, 831
Alw21I GWGCWC 1 cut(s) 791
Alw26I GTCTC 4 cut(s) 111, 353, 388, 627
AlwI GGATC 1 cut(s) 224
AoxI GGCC 2 cut(s) 3, 78
ApeKI GCWGC 4 cut(s) 471, 474, 719, 831
ApoI RAATTY 1 cut(s) 25
AseI ATTAAT 1 cut(s) 222
AspLEI GCGC 1 cut(s) 258
AsuHPI GGTGA 2 cut(s) 494, 595
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 147
BanII GRGCYC 2 cut(s) 163, 791
Bbv12I GWGCWC 1 cut(s) 791
BbvI GCAGC 4 cut(s) 458, 461, 731, 818
BccI CCATC 3 cut(s) 13, 49, 267
BceAI ACGGC 1 cut(s) 574
BcoDI GTCTC 4 cut(s) 111, 353, 388, 627
BfmI CTRYAG 1 cut(s) 424
BisI GCNGC 5 cut(s) 151, 472, 475, 720, 832
BlsI GCNGC 5 cut(s) 152, 473, 476, 721, 833
BmiI GGNNCC 3 cut(s) 149, 162, 759
BmsI GCATC 3 cut(s) 535, 576, 720
BpuEI CTTGAG 2 cut(s) 550, 811
BsaI GGTCTC 1 cut(s) 627
BsaJI CCNNGG 2 cut(s) 409, 687
Bsc4I CCNNNNNNNGG 5 cut(s) 47, 157, 511, 693, 811
Bse3DI GCAATG 5 cut(s) 178, 193, 403, 528, 778
BseDI CCNNGG 2 cut(s) 409, 687
BseGI GGATG 2 cut(s) 131, 419
BseLI CCNNNNNNNGG 5 cut(s) 47, 157, 511, 693, 811
BseMI GCAATG 5 cut(s) 178, 193, 403, 528, 778
BseMII CTCAG 2 cut(s) 804, 830
BseXI GCAGC 4 cut(s) 458, 461, 731, 818
BsgI GTGCAG 1 cut(s) 818
Bsh1236I CGCG 1 cut(s) 256
BshFI GGCC 2 cut(s) 5, 80
BshNI GGYRCC 1 cut(s) 147
BsiHKAI GWGCWC 1 cut(s) 791
BslI CCNNNNNNNGG 5 cut(s) 47, 157, 511, 693, 811
BsmAI GTCTC 4 cut(s) 111, 353, 388, 627
BsnI GGCC 2 cut(s) 5, 80
Bso31I GGTCTC 1 cut(s) 627
Bsp1286I GDGCHC 2 cut(s) 163, 791
Bsp143I GATC 2 cut(s) 229, 714
Bsp19I CCATGG 1 cut(s) 409
BspACI CCGC 3 cut(s) 151, 254, 780
BspANI GGCC 2 cut(s) 5, 80
BspCNI CTCAG 2 cut(s) 803, 829
BspFNI CGCG 1 cut(s) 256
BspHI TCATGA 1 cut(s) 529
BspLI GGNNCC 3 cut(s) 149, 162, 759
BspMAI CTGCAG 1 cut(s) 428
BspPI GGATC 1 cut(s) 224
BspQI GCTCTTC 1 cut(s) 779
BspT107I GGYRCC 1 cut(s) 147
BspTNI GGTCTC 1 cut(s) 627
BsrDI GCAATG 5 cut(s) 178, 193, 403, 528, 778
BssECI CCNNGG 2 cut(s) 409, 687
BssMI GATC 2 cut(s) 229, 714
BssT1I CCWWGG 2 cut(s) 409, 687
Bst4CI ACNGT 4 cut(s) 329, 399, 500, 583
Bst6I CTCTTC 1 cut(s) 779
BstAPI GCANNNNNTGC 1 cut(s) 777
BstC8I GCNNGC 2 cut(s) 368, 652
BstDEI CTNAG 3 cut(s) 239, 790, 816
BstDSI CCRYGG 1 cut(s) 409
BstF5I GGATG 2 cut(s) 131, 419
BstFNI CGCG 1 cut(s) 256
BstHHI GCGC 1 cut(s) 258
BstKTI GATC 2 cut(s) 232, 717
BstMAI GTCTC 4 cut(s) 111, 353, 388, 627
BstMBI GATC 2 cut(s) 229, 714
BstMWI GCNNNNNNNGC 5 cut(s) 543, 660, 777, 786, 831
BstNSI RCATGY 3 cut(s) 275, 366, 654
BstSFI CTRYAG 1 cut(s) 424
BstUI CGCG 1 cut(s) 256
BstV1I GCAGC 4 cut(s) 458, 461, 731, 818
BstXI CCANNNNNNTGG 2 cut(s) 410, 416
BsuRI GGCC 2 cut(s) 5, 80
BtgI CCRYGG 1 cut(s) 409
BtsCI GGATG 2 cut(s) 131, 419
BtsI GCAGTG 1 cut(s) 454
BtsIMutI CAGTG 1 cut(s) 454
Cac8I GCNNGC 2 cut(s) 368, 652
CciI TCATGA 1 cut(s) 529
CfoI GCGC 1 cut(s) 258
Csp6I GTAC 2 cut(s) 400, 646
CspCI CAANNNNNGTGG 2 cut(s) 448, 483
CviAII CATG 8 cut(s) 58, 245, 272, 363, 410, 530, 651, 709
CviQI GTAC 2 cut(s) 400, 646
DdeI CTNAG 3 cut(s) 239, 790, 816
DpnI GATC 2 cut(s) 231, 716
DpnII GATC 2 cut(s) 229, 714
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 779
EarI CTCTTC 1 cut(s) 779
Ecl136II GAGCTC 1 cut(s) 789
Eco130I CCWWGG 2 cut(s) 409, 687
Eco24I GRGCYC 2 cut(s) 163, 791
Eco31I GGTCTC 1 cut(s) 627
Eco53kI GAGCTC 1 cut(s) 789
EcoICRI GAGCTC 1 cut(s) 789
EcoT14I CCWWGG 2 cut(s) 409, 687
EcoT22I ATGCAT 1 cut(s) 528
EcoT38I GRGCYC 2 cut(s) 163, 791
ErhI CCWWGG 2 cut(s) 409, 687
FaeI CATG 8 cut(s) 61, 248, 275, 366, 413, 533, 654, 712
FatI CATG 8 cut(s) 57, 244, 271, 362, 409, 529, 650, 708
FauI CCCGC 1 cut(s) 773
FauNDI CATATG 1 cut(s) 639
Fnu4HI GCNGC 5 cut(s) 151, 472, 475, 720, 832
FokI GGATG 2 cut(s) 138, 426
FriOI GRGCYC 2 cut(s) 163, 791
Fsp4HI GCNGC 5 cut(s) 151, 472, 475, 720, 832
GlaI GCGC 1 cut(s) 257
GluI GCNGC 5 cut(s) 151, 472, 475, 720, 832
HaeIII GGCC 2 cut(s) 5, 80
HhaI GCGC 1 cut(s) 258
Hin1II CATG 8 cut(s) 61, 248, 275, 366, 413, 533, 654, 712
Hin6I GCGC 1 cut(s) 256
HinP1I GCGC 1 cut(s) 256
HincII GTYRAC 1 cut(s) 325
HindII GTYRAC 1 cut(s) 325
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 2 cut(s) 420, 563
HphI GGTGA 2 cut(s) 494, 595
Hpy166II GTNNAC 4 cut(s) 131, 284, 325, 596
Hpy188I TCNGA 5 cut(s) 125, 621, 747, 793, 819
Hpy188III TCNNGA 2 cut(s) 530, 567
Hpy8I GTNNAC 4 cut(s) 131, 284, 325, 596
HpyAV CCTTC 2 cut(s) 138, 509
HpyCH4III ACNGT 4 cut(s) 329, 399, 500, 583
HpyF10VI GCNNNNNNNGC 5 cut(s) 543, 660, 777, 786, 831
HpyF3I CTNAG 3 cut(s) 239, 790, 816
Hsp92II CATG 8 cut(s) 61, 248, 275, 366, 413, 533, 654, 712
HspAI GCGC 1 cut(s) 256
Kzo9I GATC 2 cut(s) 229, 714
LguI GCTCTTC 1 cut(s) 779
LmnI GCTCC 4 cut(s) 166, 437, 518, 668
LpnPI CCDG 1 cut(s) 552
Lsp1109I GCAGC 4 cut(s) 458, 461, 731, 818
LweI GCATC 3 cut(s) 535, 576, 720
MaeIII GTNAC 3 cut(s) 61, 500, 577
MalI GATC 2 cut(s) 231, 716
MboI GATC 2 cut(s) 229, 714
MboII GAAGA 1 cut(s) 796
MhlI GDGCHC 2 cut(s) 163, 791
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 25, 177, 429, 445, 483, 752, 762
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 557
MnlI CCTC 5 cut(s) 64, 101, 298, 666, 854
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 528
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 222
MslI CAYNNNNRTG 3 cut(s) 408, 414, 507
Msp20I TGGCCA 1 cut(s) 5
MvnI CGCG 1 cut(s) 256
MwoI GCNNNNNNNGC 5 cut(s) 543, 660, 777, 786, 831
NcoI CCATGG 1 cut(s) 409
NdeI CATATG 1 cut(s) 639
NdeII GATC 2 cut(s) 229, 714
NlaIII CATG 8 cut(s) 61, 248, 275, 366, 413, 533, 654, 712
NlaIV GGNNCC 3 cut(s) 149, 162, 759
NmuCI GTSAC 2 cut(s) 61, 500
NsiI ATGCAT 1 cut(s) 528
NspI RCATGY 3 cut(s) 275, 366, 654
OliI CACNNNNGTG 1 cut(s) 507
PaeI GCATGC 1 cut(s) 654
PagI TCATGA 1 cut(s) 529
PciI ACATGT 1 cut(s) 271
PciSI GCTCTTC 1 cut(s) 779
PcsI WCGNNNNNNNCGW 1 cut(s) 587
PfeI GAWTC 1 cut(s) 420
PflMI CCANNNNNTGG 3 cut(s) 47, 693, 811
PkrI GCNGC 5 cut(s) 152, 473, 476, 721, 833
PleI GAGTC 1 cut(s) 557
PpsI GAGTC 1 cut(s) 557
PscI ACATGT 1 cut(s) 271
PshBI ATTAAT 1 cut(s) 222
Psp124BI GAGCTC 1 cut(s) 791
PspN4I GGNNCC 3 cut(s) 149, 162, 759
PstI CTGCAG 1 cut(s) 428
RsaI GTAC 2 cut(s) 401, 647
RsaNI GTAC 2 cut(s) 400, 646
RseI CAYNNNNRTG 3 cut(s) 408, 414, 507
SacI GAGCTC 1 cut(s) 791
SapI GCTCTTC 1 cut(s) 779
SaqAI TTAA 1 cut(s) 222
SatI GCNGC 5 cut(s) 151, 472, 475, 720, 832
Sau3AI GATC 2 cut(s) 229, 714
SchI GAGTC 1 cut(s) 557
SduI GDGCHC 2 cut(s) 163, 791
SetI ASST 9 cut(s) 149, 240, 309, 372, 442, 693, 724, 791, 833
SfaNI GCATC 3 cut(s) 535, 576, 720
SfcI CTRYAG 1 cut(s) 424
SmiMI CAYNNNNRTG 3 cut(s) 408, 414, 507
SmlI CTYRAG 2 cut(s) 565, 826
SmoI CTYRAG 2 cut(s) 565, 826
SphI GCATGC 1 cut(s) 654
Sse9I AATT 7 cut(s) 25, 177, 429, 445, 483, 752, 762
SsiI CCGC 3 cut(s) 151, 254, 780
SspI AATATT 2 cut(s) 220, 350
SstI GAGCTC 1 cut(s) 791
StyI CCWWGG 2 cut(s) 409, 687
TaaI ACNGT 4 cut(s) 329, 399, 500, 583
TaqI TCGA 1 cut(s) 561
TasI AATT 7 cut(s) 25, 177, 429, 445, 483, 752, 762
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 1 cut(s) 420
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TscAI CASTG 1 cut(s) 461
TseFI GTSAC 2 cut(s) 61, 500
TseI GCWGC 4 cut(s) 471, 474, 719, 831
Tsp45I GTSAC 2 cut(s) 61, 500
TspDTI ATGAA 3 cut(s) 99, 189, 219
TspRI CASTG 1 cut(s) 461
Van91I CCANNNNNTGG 3 cut(s) 47, 693, 811
VspI ATTAAT 1 cut(s) 222
XapI RAATTY 1 cut(s) 25
XceI RCATGY 3 cut(s) 275, 366, 654
Zsp2I ATGCAT 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.