MD15G1113900.v1.1

RING finger and CHY zinc finger domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005253
Physical Location & Seq
Reverse (-)
8028588 .. 8033983
5396 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1113900.v1.1.491

Sequence Viewer

Length: 882 bp
ATGCAAGATATAATGGTTAGGCAGAACGTAATGGGGGATGCTCACTGTAATATATCGAATGGCGAGTATAAGCTCAAATTTGAATCGAAGGATACTGGAGAGGCCACTGAATTGCTAGAGAAAGGATATATGCAGTATGGATGTTCACACTACCGGCGGAGGTGCCGTATTAGAGCTCCTTGCTGCAATGAGATTTTTGATTGTCGCCATTGTCATAACGAAGCTAAGAATGATATTAGCATTGATCAGAAGTATAGACATGACATACCACGCCATCAAGTGAAACAGGTGATATGTTCACTTTGCAACACTGAGCAAGAGGTTCAACAAGTTTGTATTAATTGTGGTGTATGTATGGGGAAATACTTCTGTGAGACTTGCAAGCTCTTTGATGATGATGTATCTAAGAAACAGTACCATTGCAATGGCTGTGGGATTTGCAGAATTGGGGGGCGCGAGAATTTCTTTCACTGTGACAAGTGTGGTTGCTGCTACTCAATGCTGATGAAGAACAGCCATCCCTGTGTGGAGGGAGCAATGCATCACGACTGCCCTGTTTGTTTTGAGTTCTTATTCGAAACAAGAGATGATGTTACCGTCATGCCATGCGGGCACACCATTCATAAGAATTGCTTAAACGAGATGAGGGACCATTATCAATACGCATGCCCTCTTTGCTCCAAGTCAGTTTGCGATATGTCTAAGGTATGGGAGAAATATGATATGGAGATTGCAGCTACGCCGATGCCTGAACCCTATCAAAACAAATTGGTTTCGATCCTCTGCAACGACTGTGGGAAAATCTCACAAGTGCAGTACCATGTTATGGCCCGGAAATGCCCGAACTGTAAATCGTACAACACTCGACAAACCAGAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0002039 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0008270 GO:0009056 GO:0009057 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0032991 GO:0036211 GO:0042176 GO:0042802 GO:0042803 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0046872 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051865 GO:0060255 GO:0061136 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1902494 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:1990234 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
ko04115 ko04120 ko05162 map04115 map04120 map05162
Pfam Domains
Protein Families
zf-CHY zf-RING_2 zinc_ribbon_6

Protein Analysis

294

Amino Acids

33.94

Weight (kDa)

7.39

Isoelectric Point (pI)

40.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CHY PF05495 48 - 122 5.4e-19 CHY zinc finger
zf-RING_2 PF13639 183 - 226 1.4e-08 Ring finger domain
zf-RING_5 PF14634 183 - 228 1.3e-07 zinc-RING finger domain
zf-RING_UBOX PF13445 184 - 224 3.6e-07 RING-type zinc-finger
zf-C3HC4 PF00097 184 - 226 1.8e-06 Zinc finger, C3HC4 type (RING finger)
zinc_ribbon_6 PF14599 231 - 289 2.9e-25 Zinc-ribbon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 162
AccB7I CCANNNNNTGG 1 cut(s) 826
AccII CGCG 1 cut(s) 456
AciI CCGC 2 cut(s) 157, 609
AclWI GGATC 1 cut(s) 772
AcsI RAATTY 2 cut(s) 77, 460
AfaI GTAC 3 cut(s) 416, 818, 857
AfiI CCNNNNNNNGG 1 cut(s) 826
AgsI TTSAA 2 cut(s) 83, 326
AluBI AGCT 5 cut(s) 73, 176, 224, 385, 737
AluI AGCT 5 cut(s) 73, 176, 224, 385, 737
Alw21I GWGCWC 1 cut(s) 178
Alw26I GTCTC 1 cut(s) 368
AlwI GGATC 1 cut(s) 772
AlwNI CAGNNNCTG 1 cut(s) 879
AoxI GGCC 2 cut(s) 102, 828
ApeKI GCWGC 3 cut(s) 183, 489, 734
ApoI RAATTY 2 cut(s) 77, 460
AseI ATTAAT 1 cut(s) 339
Asp700I GAANNNNTTC 1 cut(s) 365
AspLEI GCGC 1 cut(s) 456
AspS9I GGNCC 2 cut(s) 649, 829
AsuC2I CCSGG 1 cut(s) 832
AsuHPI GGTGA 1 cut(s) 301
AsuII TTCGAA 1 cut(s) 576
AvaII GGWCC 1 cut(s) 649
BaeGI GKGCMC 1 cut(s) 615
BanI GGYRCC 1 cut(s) 162
BanII GRGCYC 1 cut(s) 178
Bbv12I GWGCWC 1 cut(s) 178
BbvI GCAGC 3 cut(s) 170, 476, 746
BccI CCATC 2 cut(s) 282, 525
BceAI ACGGC 1 cut(s) 150
BciVI GTATCC 1 cut(s) 85
BclI TGATCA 1 cut(s) 244
BcnI CCSGG 1 cut(s) 832
BcoDI GTCTC 1 cut(s) 368
BfaI CTAG 1 cut(s) 116
BfuI GTATCC 1 cut(s) 85
BglI GCCNNNNNGGC 1 cut(s) 610
BisI GCNGC 3 cut(s) 184, 490, 735
BlsI GCNGC 3 cut(s) 185, 491, 736
Bme1390I CCNGG 1 cut(s) 832
Bme18I GGWCC 1 cut(s) 649
BmgT120I GGNCC 2 cut(s) 649, 829
BmiI GGNNCC 2 cut(s) 164, 650
BmrFI CCNGG 1 cut(s) 832
BmsI GCATC 3 cut(s) 28, 550, 735
BpmI CTGGAG 1 cut(s) 117
Bpu14I TTCGAA 1 cut(s) 576
BpuMI CCSGG 1 cut(s) 832
BsaXI ACNNNNNCTCC 2 cut(s) 151, 181
Bsc4I CCNNNNNNNGG 1 cut(s) 826
Bse118I RCCGGY 1 cut(s) 153
Bse1I ACTGG 1 cut(s) 100
Bse3DI GCAATG 4 cut(s) 193, 418, 430, 543
BseGI GGATG 3 cut(s) 43, 146, 517
BseLI CCNNNNNNNGG 1 cut(s) 826
BseMI GCAATG 4 cut(s) 193, 418, 430, 543
BseMII CTCAG 1 cut(s) 303
BseNI ACTGG 1 cut(s) 100
BseSI GKGCMC 1 cut(s) 615
BseXI GCAGC 3 cut(s) 170, 476, 746
BsgI GTGCAG 1 cut(s) 833
Bsh1236I CGCG 1 cut(s) 456
BshFI GGCC 2 cut(s) 104, 830
BshNI GGYRCC 1 cut(s) 162
BsiHKAI GWGCWC 1 cut(s) 178
BsiSI CCGG 2 cut(s) 154, 832
BslFI GGGAC 1 cut(s) 662
BslI CCNNNNNNNGG 1 cut(s) 826
BsmAI GTCTC 1 cut(s) 368
BsmFI GGGAC 1 cut(s) 662
BsnI GGCC 2 cut(s) 104, 830
Bsp119I TTCGAA 1 cut(s) 576
Bsp1286I GDGCHC 2 cut(s) 178, 615
Bsp143I GATC 2 cut(s) 244, 777
BspACI CCGC 2 cut(s) 157, 609
BspANI GGCC 2 cut(s) 104, 830
BspCNI CTCAG 1 cut(s) 304
BspFNI CGCG 1 cut(s) 456
BspLI GGNNCC 2 cut(s) 164, 650
BspPI GGATC 1 cut(s) 772
BspT104I TTCGAA 1 cut(s) 576
BspT107I GGYRCC 1 cut(s) 162
BsrDI GCAATG 4 cut(s) 193, 418, 430, 543
BsrFI RCCGGY 1 cut(s) 153
BsrI ACTGG 1 cut(s) 100
BssAI RCCGGY 1 cut(s) 153
BssMI GATC 2 cut(s) 244, 777
Bst4CI ACNGT 6 cut(s) 47, 414, 473, 598, 794, 848
BstBI TTCGAA 1 cut(s) 576
BstC8I GCNNGC 3 cut(s) 383, 611, 667
BstDEI CTNAG 4 cut(s) 225, 312, 405, 702
BstF5I GGATG 3 cut(s) 43, 146, 517
BstFNI CGCG 1 cut(s) 456
BstHHI GCGC 1 cut(s) 456
BstKTI GATC 2 cut(s) 247, 780
BstMAI GTCTC 1 cut(s) 368
BstMBI GATC 2 cut(s) 244, 777
BstMWI GCNNNNNNNGC 2 cut(s) 610, 675
BstNSI RCATGY 1 cut(s) 669
BstSCI CCNGG 1 cut(s) 830
BstSLI GKGCMC 1 cut(s) 615
BstUI CGCG 1 cut(s) 456
BstV1I GCAGC 3 cut(s) 170, 476, 746
BstXI CCANNNNNNTGG 1 cut(s) 425
BsuI GTATCC 1 cut(s) 85
BsuRI GGCC 2 cut(s) 104, 830
BtsCI GGATG 3 cut(s) 43, 146, 517
BtsIMutI CAGTG 4 cut(s) 43, 105, 309, 469
Cac8I GCNNGC 3 cut(s) 383, 611, 667
CaiI CAGNNNCTG 1 cut(s) 879
CfoI GCGC 1 cut(s) 456
Cfr10I RCCGGY 1 cut(s) 153
Cfr13I GGNCC 2 cut(s) 649, 829
Csp6I GTAC 3 cut(s) 415, 817, 856
CspCI CAANNNNNGTGG 4 cut(s) 412, 447, 775, 810
CviAII CATG 5 cut(s) 260, 601, 606, 666, 821
CviQI GTAC 3 cut(s) 415, 817, 856
DdeI CTNAG 4 cut(s) 225, 312, 405, 702
DpnI GATC 2 cut(s) 246, 779
DpnII GATC 2 cut(s) 244, 777
EciI GGCGGA 1 cut(s) 172
Ecl136II GAGCTC 1 cut(s) 176
Eco24I GRGCYC 1 cut(s) 178
Eco47I GGWCC 1 cut(s) 649
Eco53kI GAGCTC 1 cut(s) 176
EcoICRI GAGCTC 1 cut(s) 176
EcoT22I ATGCAT 1 cut(s) 543
EcoT38I GRGCYC 1 cut(s) 178
FaeI CATG 5 cut(s) 263, 604, 609, 669, 824
FalI AAGNNNNNCTT 2 cut(s) 617, 649
FaqI GGGAC 1 cut(s) 662
FatI CATG 5 cut(s) 259, 600, 605, 665, 820
FauI CCCGC 1 cut(s) 602
FbaI TGATCA 1 cut(s) 244
Fnu4HI GCNGC 3 cut(s) 184, 490, 735
FokI GGATG 3 cut(s) 50, 153, 504
FriOI GRGCYC 1 cut(s) 178
Fsp4HI GCNGC 3 cut(s) 184, 490, 735
FspBI CTAG 1 cut(s) 116
GlaI GCGC 1 cut(s) 455
GluI GCNGC 3 cut(s) 184, 490, 735
GsuI CTGGAG 1 cut(s) 117
HaeIII GGCC 2 cut(s) 104, 830
HapII CCGG 2 cut(s) 154, 832
HhaI GCGC 1 cut(s) 456
Hin1II CATG 5 cut(s) 263, 604, 609, 669, 824
Hin6I GCGC 1 cut(s) 454
HinP1I GCGC 1 cut(s) 454
HinfI GANTC 1 cut(s) 83
HpaII CCGG 2 cut(s) 154, 832
HphI GGTGA 1 cut(s) 301
Hpy166II GTNNAC 2 cut(s) 146, 299
Hpy188I TCNGA 1 cut(s) 249
Hpy188III TCNNGA 1 cut(s) 545
Hpy8I GTNNAC 2 cut(s) 146, 299
HpyAV CCTTC 1 cut(s) 82
HpyCH4III ACNGT 6 cut(s) 47, 414, 473, 598, 794, 848
HpyCH4IV ACGT 1 cut(s) 27
HpyF10VI GCNNNNNNNGC 2 cut(s) 610, 675
HpyF3I CTNAG 4 cut(s) 225, 312, 405, 702
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 5 cut(s) 263, 604, 609, 669, 824
HspAI GCGC 1 cut(s) 454
Ksp22I TGATCA 1 cut(s) 244
Kzo9I GATC 2 cut(s) 244, 777
LmnI GCTCC 3 cut(s) 181, 533, 683
LpnPI CCDG 7 cut(s) 81, 167, 272, 535, 567, 762, 845
Lsp1109I GCAGC 3 cut(s) 170, 476, 746
LweI GCATC 3 cut(s) 28, 550, 735
MaeI CTAG 1 cut(s) 116
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 2 cut(s) 473, 592
MalI GATC 2 cut(s) 246, 779
MboI GATC 2 cut(s) 244, 777
MboII GAAGA 1 cut(s) 520
MhlI GDGCHC 2 cut(s) 178, 615
MluCI AATT 7 cut(s) 77, 110, 340, 444, 460, 628, 767
MnlI CCTC 8 cut(s) 94, 153, 313, 523, 639, 681, 791, 869
Mph1103I ATGCAT 1 cut(s) 543
MroXI GAANNNNTTC 1 cut(s) 365
MseI TTAA 2 cut(s) 339, 635
MslI CAYNNNNRTG 2 cut(s) 423, 522
MspI CCGG 2 cut(s) 154, 832
MspR9I CCNGG 1 cut(s) 832
MvnI CGCG 1 cut(s) 456
MwoI GCNNNNNNNGC 2 cut(s) 610, 675
NciI CCSGG 1 cut(s) 832
NdeII GATC 2 cut(s) 244, 777
NlaIII CATG 5 cut(s) 263, 604, 609, 669, 824
NlaIV GGNNCC 2 cut(s) 164, 650
NmuCI GTSAC 1 cut(s) 473
NsiI ATGCAT 1 cut(s) 543
NspI RCATGY 1 cut(s) 669
NspV TTCGAA 1 cut(s) 576
PaeI GCATGC 1 cut(s) 669
PdmI GAANNNNTTC 1 cut(s) 365
PfeI GAWTC 1 cut(s) 83
PflMI CCANNNNNTGG 1 cut(s) 826
PkrI GCNGC 3 cut(s) 185, 491, 736
PshBI ATTAAT 1 cut(s) 339
Psp124BI GAGCTC 1 cut(s) 178
PspN4I GGNNCC 2 cut(s) 164, 650
PspPI GGNCC 2 cut(s) 649, 829
PstNI CAGNNNCTG 1 cut(s) 879
RsaI GTAC 3 cut(s) 416, 818, 857
RsaNI GTAC 3 cut(s) 415, 817, 856
RseI CAYNNNNRTG 2 cut(s) 423, 522
SacI GAGCTC 1 cut(s) 178
SaqAI TTAA 2 cut(s) 339, 635
SatI GCNGC 3 cut(s) 184, 490, 735
Sau3AI GATC 2 cut(s) 244, 777
Sau96I GGNCC 2 cut(s) 649, 829
ScrFI CCNGG 1 cut(s) 832
SduI GDGCHC 2 cut(s) 178, 615
SfaNI GCATC 3 cut(s) 28, 550, 735
SfuI TTCGAA 1 cut(s) 576
SinI GGWCC 1 cut(s) 649
SmiMI CAYNNNNRTG 2 cut(s) 423, 522
SphI GCATGC 1 cut(s) 669
Sse9I AATT 7 cut(s) 77, 110, 340, 444, 460, 628, 767
SsiI CCGC 2 cut(s) 157, 609
SspMI CTAG 1 cut(s) 116
SstI GAGCTC 1 cut(s) 178
StyD4I CCNGG 1 cut(s) 830
TaaI ACNGT 6 cut(s) 47, 414, 473, 598, 794, 848
TaiI ACGT 1 cut(s) 30
TaqI TCGA 5 cut(s) 56, 86, 576, 776, 865
TasI AATT 7 cut(s) 77, 110, 340, 444, 460, 628, 767
TfiI GAWTC 1 cut(s) 83
Tru1I TTAA 2 cut(s) 339, 635
Tru9I TTAA 2 cut(s) 339, 635
TscAI CASTG 4 cut(s) 50, 112, 316, 476
TseFI GTSAC 1 cut(s) 473
TseI GCWGC 3 cut(s) 183, 489, 734
Tsp45I GTSAC 1 cut(s) 473
TspDTI ATGAA 2 cut(s) 521, 611
TspRI CASTG 4 cut(s) 50, 112, 316, 476
Van91I CCANNNNNTGG 1 cut(s) 826
VpaK11BI GGWCC 1 cut(s) 649
VspI ATTAAT 1 cut(s) 339
XapI RAATTY 2 cut(s) 77, 460
XceI RCATGY 1 cut(s) 669
XmnI GAANNNNTTC 1 cut(s) 365
XspI CTAG 1 cut(s) 116
Zsp2I ATGCAT 1 cut(s) 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.