AT5G27660

Trypsin-like serine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005022
Physical Location & Seq
Reverse (-)
9789468 .. 9792378
2911 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G27660.2

Sequence Viewer

Length: 1275 bp
AGAAGAGCTGTATCATCTTCTAAAAGAAGCGAGCTTATTCGCATCATTTCTGTTGCTACTGCTACTTCTGGAATTTTATATGCTAGTACTAATCCAGATGCAAGAACTCGAGTATCGCTTGCTATACCTGAATCTGTTCGAGAATCTTTGTCGTTACTGCCTTGGCAGATTTCACCGGGTTTGATTCATCGTCCTGAGCAAAGCTTATTCGGAAATTTCGTGTTTTCTTCGAGAGTTAGTCCGAAATCAGAAGCACCTATAAATGATGAGAAGGGAGTTTCTGTTGAAGCTTCGGATAGTAGTTCGAAACCAAGTAATGGCTACTTAGGGAGAGATACTATAGCCAATGCGGCTGCTAGAATTGGACCAGCGGTTGTCAATCTTTCGGTTCCTCAGGGTTTCCATGGGATATCTATGGGGAAAAGTATCGGTTCTGGAACGATCATTGATGCTGATGGCACGATACTGACTTGCGCTCATGTTGTAGTTGATTTTCAGAACATTCGTCATTCTTCTAAAGGGAGGGTAGATGTGACGTTGCAAGATGGTAGGACGTTTGAGGGTGTGGTGGTGAACGCTGATTTACAATCCGACATTGCATTAGTGAAGATAAAGTCAAAGACGCCATTGCCAACTGCTAAACTTGGTTTTTCGAGTAAGCTTCGTCCTGGAGACTGGGTAATAGCGGTAGGTTGTCCTCTGTCTCTCCAGAACACAGTAACTGCTGGTATTGTCAGTTGTGTTGATCGCAAAAGCAGTGATTTGGGTTTAGGAGGCAAACATAGAGAGTATCTCCAAACAGACTGCTCGATCAATGCGGGAAACTCTGGTGGGCCTCTTGTAAATTTGGATGGGGAAGTGATTGGTGTTAATATCATGAAAGTTTTAGCTGCAGATGGTCTTGGATTTTCCGTGCCAATTGACTCGGTATCTAAAATCATCGAGCATTTCAAGAAGAGTGGTAGAGTTATTCGGCCATGGATTGGGCTCAAAATGGTCGAACTCAATAACTTGATCGTTGCTCAGCTAAAAGAACGAGACCCAATGTTTCCTGATGTCGAAAGAGGCGTTCTTGTTCCTACGGTGATTCCGGGATCACCGGCTGATCGAGCTGGCTTCAAACCAGGAGATGTTGTTGTGAGATTTGATGGGAAGCCGGTGATAGAGATAATGGACGACAGAGTTGGGAAGAGGATGCAAGTAGTTGTGGAAAGATCAAATAAAGAAAGGGTCACACTAGAAGTCATTCCTGAGGAGGCTAATCCAGACATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04210 ko04214 ko04215 ko05012 map04210 map04214 map04215 map05012
Pfam Domains
Protein Families
PDZ_2 Trypsin_2

Protein Analysis

424

Amino Acids

45.31

Weight (kDa)

8.79

Isoelectric Point (pI)

36.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trypsin PF00089 128 - 319 3.5e-19 Trypsin
Trypsin_2 PF13365 149 - 295 1.5e-34 Trypsin-like peptidase domain
PDZ_2 PF13180 333 - 425 9.2e-12 PDZ domain
PDZ_6 PF17820 364 - 416 1.6e-13 PDZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 317, 983
AciI CCGC 4 cut(s) 350, 371, 686, 818
AclWI GGATC 1 cut(s) 1102
AcoI YGGCCR 1 cut(s) 974
AcsI RAATTY 3 cut(s) 72, 214, 844
AcyI GRCGYC 1 cut(s) 623
AfaI GTAC 1 cut(s) 88
AfiI CCNNNNNNNGG 2 cut(s) 317, 983
AflIII ACRYGT 1 cut(s) 1269
AgsI TTSAA 3 cut(s) 287, 952, 1120
AjnI CCWGG 2 cut(s) 667, 1123
AluBI AGCT 8 cut(s) 8, 34, 204, 290, 661, 890, 1027, 1112
AluI AGCT 8 cut(s) 8, 34, 204, 290, 661, 890, 1027, 1112
Alw26I GTCTC 3 cut(s) 666, 708, 1032
AlwI GGATC 1 cut(s) 1102
AlwNI CAGNNNCTG 1 cut(s) 722
Ama87I CYCGRG 1 cut(s) 108
AoxI GGCC 2 cut(s) 833, 974
ApeKI GCWGC 2 cut(s) 353, 890
ApoI RAATTY 3 cut(s) 72, 214, 844
Asp700I GAANNNNTTC 2 cut(s) 135, 1245
AspLEI GCGC 1 cut(s) 476
AspS9I GGNCC 2 cut(s) 365, 833
AsuC2I CCSGG 2 cut(s) 177, 1092
AsuHPI GGTGA 5 cut(s) 165, 583, 1089, 1096, 1171
AsuII TTCGAA 1 cut(s) 305
AvaI CYCGRG 1 cut(s) 108
AvaII GGWCC 1 cut(s) 365
AxyI CCTNAGG 2 cut(s) 393, 1251
BaeI ACNNNNGTAYC 2 cut(s) 96, 129
BanII GRGCYC 1 cut(s) 990
BbvI GCAGC 2 cut(s) 340, 877
BccI CCATC 5 cut(s) 449, 539, 845, 890, 1142
BciT130I CCWGG 2 cut(s) 669, 1125
BcnI CCSGG 2 cut(s) 177, 1092
BcoDI GTCTC 3 cut(s) 666, 708, 1032
BfaI CTAG 3 cut(s) 84, 357, 1238
BfmI CTRYAG 2 cut(s) 339, 891
BglI GCCNNNNNGGC 1 cut(s) 350
BisI GCNGC 3 cut(s) 351, 354, 891
BlpI GCTNAGC 1 cut(s) 1023
BlsI GCNGC 3 cut(s) 352, 355, 892
BmcAI AGTACT 1 cut(s) 88
Bme1390I CCNGG 4 cut(s) 177, 669, 1092, 1125
Bme18I GGWCC 1 cut(s) 365
BmeT110I CYCGRG 1 cut(s) 108
BmgT120I GGNCC 2 cut(s) 365, 833
BmiI GGNNCC 1 cut(s) 390
BmrFI CCNGG 4 cut(s) 177, 669, 1092, 1125
BmrI ACTGGG 1 cut(s) 685
BmsI GCATC 4 cut(s) 51, 88, 439, 1185
BmuI ACTGGG 1 cut(s) 685
BplI GAGNNNNNCTC 2 cut(s) 777, 809
BpmI CTGGAG 2 cut(s) 690, 692
Bpu10I CCTNAGC 1 cut(s) 195
Bpu1102I GCTNAGC 1 cut(s) 1023
Bpu14I TTCGAA 1 cut(s) 305
BpuMI CCSGG 2 cut(s) 177, 1092
BsaHI GRCGYC 1 cut(s) 623
BsaI GGTCTC 1 cut(s) 1032
BsaJI CCNNGG 3 cut(s) 161, 403, 977
BsaXI ACNNNNNCTCC 6 cut(s) 267, 297, 663, 693, 1119, 1149
Bsc4I CCNNNNNNNGG 2 cut(s) 317, 983
Bse118I RCCGGY 2 cut(s) 1099, 1156
Bse1I ACTGG 1 cut(s) 680
Bse21I CCTNAGG 2 cut(s) 393, 1251
Bse3DI GCAATG 2 cut(s) 594, 626
BseBI CCWGG 2 cut(s) 669, 1125
BseDI CCNNGG 3 cut(s) 161, 403, 977
BseGI GGATG 2 cut(s) 856, 1200
BseLI CCNNNNNNNGG 2 cut(s) 317, 983
BseMI GCAATG 2 cut(s) 594, 626
BseMII CTCAG 4 cut(s) 186, 407, 1037, 1242
BseNI ACTGG 1 cut(s) 680
BseRI GAGGAG 1 cut(s) 1268
BseXI GCAGC 2 cut(s) 340, 877
BshFI GGCC 2 cut(s) 835, 976
BsiHKCI CYCGRG 1 cut(s) 108
BsiSI CCGG 4 cut(s) 176, 1091, 1100, 1157
BslI CCNNNNNNNGG 2 cut(s) 317, 983
BsmAI GTCTC 3 cut(s) 666, 708, 1032
BsnI GGCC 2 cut(s) 835, 976
Bso31I GGTCTC 1 cut(s) 1032
BsoBI CYCGRG 1 cut(s) 108
Bsp119I TTCGAA 1 cut(s) 305
Bsp1286I GDGCHC 1 cut(s) 990
Bsp143I GATC 7 cut(s) 441, 745, 810, 1014, 1094, 1105, 1214
Bsp1720I GCTNAGC 1 cut(s) 1023
Bsp19I CCATGG 2 cut(s) 403, 977
BspACI CCGC 4 cut(s) 350, 371, 686, 818
BspANI GGCC 2 cut(s) 835, 976
BspCNI CTCAG 4 cut(s) 187, 406, 1036, 1243
BspHI TCATGA 1 cut(s) 876
BspLI GGNNCC 1 cut(s) 390
BspMAI CTGCAG 1 cut(s) 895
BspPI GGATC 1 cut(s) 1102
BspT104I TTCGAA 1 cut(s) 305
BspTNI GGTCTC 1 cut(s) 1032
BsrDI GCAATG 2 cut(s) 594, 626
BsrFI RCCGGY 2 cut(s) 1099, 1156
BsrI ACTGG 1 cut(s) 680
BssAI RCCGGY 2 cut(s) 1099, 1156
BssECI CCNNGG 3 cut(s) 161, 403, 977
BssMI GATC 7 cut(s) 441, 745, 810, 1014, 1094, 1105, 1214
BssNI GRCGYC 1 cut(s) 623
BssT1I CCWWGG 3 cut(s) 161, 403, 977
Bst2UI CCWGG 2 cut(s) 669, 1125
Bst4CI ACNGT 2 cut(s) 718, 1084
Bst6I CTCTTC 2 cut(s) 950, 1184
BstACI GRCGYC 1 cut(s) 623
BstBI TTCGAA 1 cut(s) 305
BstC8I GCNNGC 3 cut(s) 32, 120, 1114
BstDEI CTNAG 5 cut(s) 195, 325, 393, 1023, 1251
BstDSI CCRYGG 2 cut(s) 403, 977
BstF5I GGATG 2 cut(s) 856, 1200
BstHHI GCGC 1 cut(s) 476
BstKTI GATC 7 cut(s) 444, 748, 813, 1017, 1097, 1108, 1217
BstMAI GTCTC 3 cut(s) 666, 708, 1032
BstMBI GATC 7 cut(s) 441, 745, 810, 1014, 1094, 1105, 1214
BstMWI GCNNNNNNNGC 2 cut(s) 350, 1109
BstNI CCWGG 2 cut(s) 669, 1125
BstNSI RCATGY 1 cut(s) 1273
BstSCI CCNGG 4 cut(s) 175, 667, 1090, 1123
BstSFI CTRYAG 2 cut(s) 339, 891
BstV1I GCAGC 2 cut(s) 340, 877
Bsu36I CCTNAGG 2 cut(s) 393, 1251
BsuRI GGCC 2 cut(s) 835, 976
BtgI CCRYGG 2 cut(s) 403, 977
BtsCI GGATG 2 cut(s) 856, 1200
BtsI GCAGTG 1 cut(s) 763
BtsIMutI CAGTG 1 cut(s) 763
Cac8I GCNNGC 3 cut(s) 32, 120, 1114
CaiI CAGNNNCTG 1 cut(s) 722
CciI TCATGA 1 cut(s) 876
CfoI GCGC 1 cut(s) 476
Cfr10I RCCGGY 2 cut(s) 1099, 1156
Cfr13I GGNCC 2 cut(s) 365, 833
CseI GACGC 1 cut(s) 631
Csp6I GTAC 1 cut(s) 87
CspCI CAANNNNNGTGG 2 cut(s) 940, 975
CviAII CATG 5 cut(s) 404, 479, 877, 978, 1270
CviQI GTAC 1 cut(s) 87
DdeI CTNAG 5 cut(s) 195, 325, 393, 1023, 1251
DpnI GATC 7 cut(s) 443, 747, 812, 1016, 1096, 1107, 1216
DpnII GATC 7 cut(s) 441, 745, 810, 1014, 1094, 1105, 1214
EaeI YGGCCR 1 cut(s) 974
Eam1104I CTCTTC 2 cut(s) 950, 1184
EarI CTCTTC 2 cut(s) 950, 1184
Eco130I CCWWGG 3 cut(s) 161, 403, 977
Eco24I GRGCYC 1 cut(s) 990
Eco31I GGTCTC 1 cut(s) 1032
Eco32I GATATC 1 cut(s) 411
Eco47I GGWCC 1 cut(s) 365
Eco81I CCTNAGG 2 cut(s) 393, 1251
Eco88I CYCGRG 1 cut(s) 108
EcoRII CCWGG 2 cut(s) 667, 1123
EcoRV GATATC 1 cut(s) 411
EcoT14I CCWWGG 3 cut(s) 161, 403, 977
EcoT38I GRGCYC 1 cut(s) 990
ErhI CCWWGG 3 cut(s) 161, 403, 977
FaeI CATG 5 cut(s) 407, 482, 880, 981, 1273
FatI CATG 5 cut(s) 403, 478, 876, 977, 1269
FauI CCCGC 1 cut(s) 811
Fnu4HI GCNGC 3 cut(s) 351, 354, 891
FokI GGATG 2 cut(s) 863, 1207
FriOI GRGCYC 1 cut(s) 990
Fsp4HI GCNGC 3 cut(s) 351, 354, 891
FspBI CTAG 3 cut(s) 84, 357, 1238
GlaI GCGC 1 cut(s) 475
GluI GCNGC 3 cut(s) 351, 354, 891
GsuI CTGGAG 2 cut(s) 690, 692
HaeIII GGCC 2 cut(s) 835, 976
HapII CCGG 4 cut(s) 176, 1091, 1100, 1157
HgaI GACGC 1 cut(s) 631
HhaI GCGC 1 cut(s) 476
Hin1I GRCGYC 1 cut(s) 623
Hin1II CATG 5 cut(s) 407, 482, 880, 981, 1273
Hin6I GCGC 1 cut(s) 474
HinP1I GCGC 1 cut(s) 474
HindIII AAGCTT 3 cut(s) 202, 288, 659
HinfI GANTC 5 cut(s) 131, 143, 184, 923, 1087
HpaII CCGG 4 cut(s) 176, 1091, 1100, 1157
HphI GGTGA 5 cut(s) 165, 583, 1089, 1096, 1171
Hpy166II GTNNAC 1 cut(s) 574
Hpy188I TCNGA 6 cut(s) 212, 243, 250, 295, 498, 592
Hpy8I GTNNAC 1 cut(s) 574
HpyAV CCTTC 1 cut(s) 265
HpyCH4III ACNGT 2 cut(s) 718, 1084
HpyCH4IV ACGT 2 cut(s) 536, 554
HpyCH4V TGCA 5 cut(s) 101, 541, 599, 893, 1198
HpyF10VI GCNNNNNNNGC 2 cut(s) 350, 1109
HpyF3I CTNAG 5 cut(s) 195, 325, 393, 1023, 1251
HpySE526I ACGT 2 cut(s) 536, 554
Hsp92I GRCGYC 1 cut(s) 623
Hsp92II CATG 5 cut(s) 407, 482, 880, 981, 1273
HspAI GCGC 1 cut(s) 474
Kzo9I GATC 7 cut(s) 441, 745, 810, 1014, 1094, 1105, 1214
Lsp1109I GCAGC 2 cut(s) 340, 877
LweI GCATC 4 cut(s) 51, 88, 439, 1185
MaeI CTAG 3 cut(s) 84, 357, 1238
MaeII ACGT 2 cut(s) 536, 554
MaeIII GTNAC 4 cut(s) 153, 532, 718, 1231
MalI GATC 7 cut(s) 443, 747, 812, 1016, 1096, 1107, 1216
MboI GATC 7 cut(s) 441, 745, 810, 1014, 1094, 1105, 1214
MboII GAAGA 7 cut(s) 9, 15, 219, 504, 619, 967, 1201
MfeI CAATTG 1 cut(s) 918
MhlI GDGCHC 1 cut(s) 990
MluCI AATT 5 cut(s) 72, 214, 360, 844, 918
MlyI GAGTC 1 cut(s) 917
MmeI TCCRAC 1 cut(s) 615
MroXI GAANNNNTTC 2 cut(s) 135, 1245
MseI TTAA 1 cut(s) 870
MspA1I CMGCKG 1 cut(s) 371
MspI CCGG 4 cut(s) 176, 1091, 1100, 1157
MspR9I CCNGG 4 cut(s) 177, 669, 1092, 1125
MunI CAATTG 1 cut(s) 918
MvaI CCWGG 2 cut(s) 669, 1125
MwoI GCNNNNNNNGC 2 cut(s) 350, 1109
NciI CCSGG 2 cut(s) 177, 1092
NcoI CCATGG 2 cut(s) 403, 977
NdeII GATC 7 cut(s) 441, 745, 810, 1014, 1094, 1105, 1214
NlaIII CATG 5 cut(s) 407, 482, 880, 981, 1273
NlaIV GGNNCC 1 cut(s) 390
NmuCI GTSAC 2 cut(s) 532, 1231
NspI RCATGY 1 cut(s) 1273
NspV TTCGAA 1 cut(s) 305
PaeR7I CTCGAG 1 cut(s) 108
PagI TCATGA 1 cut(s) 876
PciI ACATGT 1 cut(s) 1269
PcsI WCGNNNNNNNCGW 2 cut(s) 216, 1065
PdmI GAANNNNTTC 2 cut(s) 135, 1245
PfeI GAWTC 4 cut(s) 131, 143, 184, 1087
PflMI CCANNNNNTGG 2 cut(s) 317, 983
PfoI TCCNGGA 2 cut(s) 667, 1090
PkrI GCNGC 3 cut(s) 352, 355, 892
PleI GAGTC 1 cut(s) 917
PpsI GAGTC 1 cut(s) 917
PscI ACATGT 1 cut(s) 1269
Psp6I CCWGG 2 cut(s) 667, 1123
PspGI CCWGG 2 cut(s) 667, 1123
PspN4I GGNNCC 1 cut(s) 390
PspPI GGNCC 2 cut(s) 365, 833
PspXI VCTCGAGB 1 cut(s) 108
PstI CTGCAG 1 cut(s) 895
PstNI CAGNNNCTG 1 cut(s) 722
RsaI GTAC 1 cut(s) 88
RsaNI GTAC 1 cut(s) 87
SaqAI TTAA 1 cut(s) 870
SatI GCNGC 3 cut(s) 351, 354, 891
Sau3AI GATC 7 cut(s) 441, 745, 810, 1014, 1094, 1105, 1214
Sau96I GGNCC 2 cut(s) 365, 833
ScaI AGTACT 1 cut(s) 88
SchI GAGTC 1 cut(s) 917
ScrFI CCNGG 4 cut(s) 177, 669, 1092, 1125
SduI GDGCHC 1 cut(s) 990
SfaNI GCATC 4 cut(s) 51, 88, 439, 1185
SfcI CTRYAG 2 cut(s) 339, 891
Sfr274I CTCGAG 1 cut(s) 108
SfuI TTCGAA 1 cut(s) 305
SinI GGWCC 1 cut(s) 365
SlaI CTCGAG 1 cut(s) 108
SmlI CTYRAG 1 cut(s) 108
SmoI CTYRAG 1 cut(s) 108
Sse9I AATT 5 cut(s) 72, 214, 360, 844, 918
SsiI CCGC 4 cut(s) 350, 371, 686, 818
SspMI CTAG 3 cut(s) 84, 357, 1238
StyD4I CCNGG 4 cut(s) 175, 667, 1090, 1123
StyI CCWWGG 3 cut(s) 161, 403, 977
TaaI ACNGT 2 cut(s) 718, 1084
TaiI ACGT 2 cut(s) 539, 557
TasI AATT 5 cut(s) 72, 214, 360, 844, 918
TatI WGTACW 1 cut(s) 86
TauI GCSGC 1 cut(s) 353
TfiI GAWTC 4 cut(s) 131, 143, 184, 1087
Tru1I TTAA 1 cut(s) 870
Tru9I TTAA 1 cut(s) 870
TscAI CASTG 1 cut(s) 763
TseFI GTSAC 2 cut(s) 532, 1231
TseI GCWGC 2 cut(s) 353, 890
Tsp45I GTSAC 2 cut(s) 532, 1231
TspDTI ATGAA 2 cut(s) 176, 893
TspGWI ACGGA 1 cut(s) 901
TspRI CASTG 1 cut(s) 763
Van91I CCANNNNNTGG 2 cut(s) 317, 983
VpaK11BI GGWCC 1 cut(s) 365
XapI RAATTY 3 cut(s) 72, 214, 844
XceI RCATGY 1 cut(s) 1273
XhoI CTCGAG 1 cut(s) 108
XmnI GAANNNNTTC 2 cut(s) 135, 1245
XspI CTAG 3 cut(s) 84, 357, 1238
ZrmI AGTACT 1 cut(s) 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.