RLG00000025356

protease Do-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0005022
Physical Location & Seq
Forward (+)
45248123 .. 45259016
10894 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025356

Sequence Viewer

Length: 1005 bp
ATGGAGGATAGTGGGATCTATTCCTACACCAATGAGGGAATCATTGCTGTTTCCATGGAAAACCAAGCAATGGAATCCATGCCACTTTGGTTAGTCCACTCCCTTCATTTGATTCTTTTGTTCATTTTCGAAAACTACATTGTGTTGTTTTCAATGTATTGTTTTCGTGATGGTTCGGTTCCTCCACCGGACATAAGGAAAGATGGTACTTCTGGTGCCCTTATAGCCGGGGAGAGGCCGGAGCGTACTTATGCCAGTGCATTTGCTAAGATTAGCCTTGCCGTGGTGCATATTCATGCTCCAACTAGGGGCCATTGCTCAGGAACAATCTTGACATGCGCTCATGCTTTTGTTGATTTAAGAAGATTTGGAGATTCGTATGAGGGGAAGATGGTCGGATGTGAGGGTACTGTGGTGAATGTGGATGCGCACTCAGACATTGCTATTATCAAAATCAATTCTAACATTCCACTGCCATCAGCAGAACTTGGTCGTTCAAGTAAAGTTCAACCAGGGGACTGTGTCATAGCGGTAAACATAACTGTGTCATTGCCTTGTCTGATTCTTTTCTCTCTTGAATCTAGTTATGCTGCTCGTAAAAACAGTGAAATGAGAGCTGGTGGGTGGGGAGAGTGTCGAGAGTACATACAGACAGATTGCTCAATCAACCCGGGAAATTCGGGAGGGCCCCTTGTAAATATGGATGGAGAAGTTATTGCTGTTAATGTTATGATGATACGACCTGCTGATGGGTTGAGTTTCGCCGTACCAATTGATTCAGTCGCTAAAGTTATGGATCAGTTCAAGAAAAATGGGAGGGCAGTTCGGCCTTGGCTTGGAGTTAAAATGATTGAGCTTAATGATGTAATAATCTCTCAGCTTGAGGAAAGAGGTTCTGCATTTCCAAATGTAAAGAAAGGAATATTTGTGCCTATGGTAACTCTTGAGTCACCCGGTGAGCGTATTGGGTTCCGTCCTGGTGACGTTGTCTCCGAATTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04210 ko04214 ko04215 ko05012 map04210 map04214 map04215 map05012
Pfam Domains
Protein Families
PDZ_2 Trypsin_2

Protein Analysis

335

Amino Acids

36.33

Weight (kDa)

5.34

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trypsin_2 PF13365 108 - 241 5.7e-18 Trypsin-like peptidase domain
Trypsin PF00089 110 - 264 1.4e-09 Trypsin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 429
Acc36I ACCTGC 1 cut(s) 751
AccB1I GGYRCC 1 cut(s) 215
AccB7I CCANNNNNTGG 1 cut(s) 70
AciI CCGC 1 cut(s) 530
AclWI GGATC 2 cut(s) 23, 804
AcsI RAATTY 2 cut(s) 676, 995
AdeI CACNNNGTG 1 cut(s) 956
AfaI GTAC 5 cut(s) 208, 247, 409, 644, 768
AfiI CCNNNNNNNGG 6 cut(s) 70, 234, 283, 308, 749, 836
AgsI TTSAA 5 cut(s) 153, 498, 509, 578, 805
AjnI CCWGG 2 cut(s) 511, 976
AluBI AGCT 3 cut(s) 617, 856, 880
AluI AGCT 3 cut(s) 617, 856, 880
Alw26I GTCTC 1 cut(s) 994
AlwI GGATC 2 cut(s) 23, 804
Ama87I CYCGRG 1 cut(s) 670
AoxI GGCC 4 cut(s) 236, 310, 686, 827
ApaI GGGCCC 1 cut(s) 690
ApeKI GCWGC 1 cut(s) 590
ApoI RAATTY 2 cut(s) 676, 995
AspLEI GCGC 2 cut(s) 341, 430
AspS9I GGNCC 3 cut(s) 310, 686, 687
AsuC2I CCSGG 4 cut(s) 229, 671, 672, 954
AsuHPI GGTGA 4 cut(s) 427, 942, 968, 992
AsuII TTCGAA 1 cut(s) 129
AvaI CYCGRG 1 cut(s) 670
BaeGI GKGCMC 2 cut(s) 220, 690
BaeI ACNNNNGTAYC 2 cut(s) 399, 432
BanI GGYRCC 1 cut(s) 215
BanII GRGCYC 1 cut(s) 690
BbvI GCAGC 1 cut(s) 577
BccI CCATC 6 cut(s) 164, 197, 385, 484, 698, 743
BceAI ACGGC 2 cut(s) 266, 749
BciT130I CCWGG 2 cut(s) 513, 978
BcnI CCSGG 4 cut(s) 229, 671, 672, 954
BcoDI GTCTC 1 cut(s) 994
BfaI CTAG 2 cut(s) 306, 582
BfuAI ACCTGC 1 cut(s) 751
BisI GCNGC 1 cut(s) 591
BlsI GCNGC 1 cut(s) 592
Bme1390I CCNGG 6 cut(s) 229, 513, 671, 672, 954, 978
BmeT110I CYCGRG 1 cut(s) 670
BmgT120I GGNCC 3 cut(s) 310, 686, 687
BmiI GGNNCC 6 cut(s) 180, 217, 311, 688, 689, 971
BmrFI CCNGG 6 cut(s) 229, 513, 671, 672, 954, 978
BmsI GCATC 1 cut(s) 415
Bpu10I CCTNAGC 1 cut(s) 319
Bpu14I TTCGAA 1 cut(s) 129
BpuEI CTTGAG 2 cut(s) 902, 965
BpuMI CCSGG 4 cut(s) 229, 671, 672, 954
BsaJI CCNNGG 6 cut(s) 54, 228, 282, 512, 670, 830
BsaWI WCCGGW 1 cut(s) 187
BsaXI ACNNNNNCTCC 2 cut(s) 974, 1004
Bsc4I CCNNNNNNNGG 6 cut(s) 70, 234, 283, 308, 749, 836
Bse1I ACTGG 1 cut(s) 255
Bse3DI GCAATG 5 cut(s) 42, 75, 313, 438, 548
BseBI CCWGG 2 cut(s) 513, 978
BseDI CCNNGG 6 cut(s) 54, 228, 282, 512, 670, 830
BseGI GGATG 3 cut(s) 404, 430, 709
BseLI CCNNNNNNNGG 6 cut(s) 70, 234, 283, 308, 749, 836
BseMI GCAATG 5 cut(s) 42, 75, 313, 438, 548
BseMII CTCAG 3 cut(s) 333, 447, 890
BseNI ACTGG 1 cut(s) 255
BseSI GKGCMC 2 cut(s) 220, 690
BseXI GCAGC 1 cut(s) 577
BshFI GGCC 4 cut(s) 238, 312, 688, 829
BshNI GGYRCC 1 cut(s) 215
BsiHKCI CYCGRG 1 cut(s) 670
BsiSI CCGG 5 cut(s) 188, 228, 239, 671, 954
BslFI GGGAC 1 cut(s) 530
BslI CCNNNNNNNGG 6 cut(s) 70, 234, 283, 308, 749, 836
BsmAI GTCTC 1 cut(s) 994
BsmFI GGGAC 1 cut(s) 530
BsnI GGCC 4 cut(s) 238, 312, 688, 829
BsoBI CYCGRG 1 cut(s) 670
Bsp119I TTCGAA 1 cut(s) 129
Bsp120I GGGCCC 1 cut(s) 686
Bsp1286I GDGCHC 2 cut(s) 220, 690
Bsp143I GATC 2 cut(s) 15, 796
Bsp19I CCATGG 1 cut(s) 54
BspACI CCGC 1 cut(s) 530
BspANI GGCC 4 cut(s) 238, 312, 688, 829
BspCNI CTCAG 3 cut(s) 332, 446, 889
BspLI GGNNCC 6 cut(s) 180, 217, 311, 688, 689, 971
BspMI ACCTGC 1 cut(s) 751
BspPI GGATC 2 cut(s) 23, 804
BspT104I TTCGAA 1 cut(s) 129
BspT107I GGYRCC 1 cut(s) 215
BsrDI GCAATG 5 cut(s) 42, 75, 313, 438, 548
BsrI ACTGG 1 cut(s) 255
BssECI CCNNGG 6 cut(s) 54, 228, 282, 512, 670, 830
BssMI GATC 2 cut(s) 15, 796
BssT1I CCWWGG 2 cut(s) 54, 830
Bst2UI CCWGG 2 cut(s) 513, 978
Bst4CI ACNGT 4 cut(s) 412, 521, 544, 605
BstBI TTCGAA 1 cut(s) 129
BstDEI CTNAG 4 cut(s) 267, 319, 433, 876
BstDSI CCRYGG 2 cut(s) 54, 282
BstF5I GGATG 3 cut(s) 404, 430, 709
BstHHI GCGC 2 cut(s) 341, 430
BstKTI GATC 2 cut(s) 18, 799
BstMAI GTCTC 1 cut(s) 994
BstMBI GATC 2 cut(s) 15, 796
BstMWI GCNNNNNNNGC 1 cut(s) 224
BstNI CCWGG 2 cut(s) 513, 978
BstNSI RCATGY 1 cut(s) 339
BstSCI CCNGG 6 cut(s) 227, 511, 669, 670, 952, 976
BstSLI GKGCMC 2 cut(s) 220, 690
BstV1I GCAGC 1 cut(s) 577
BstX2I RGATCY 1 cut(s) 15
BstYI RGATCY 1 cut(s) 15
BsuRI GGCC 4 cut(s) 238, 312, 688, 829
BtgI CCRYGG 2 cut(s) 54, 282
BtsCI GGATG 3 cut(s) 404, 430, 709
BtsI GCAGTG 1 cut(s) 470
BtsIMutI CAGTG 3 cut(s) 262, 470, 610
BveI ACCTGC 1 cut(s) 751
CfoI GCGC 2 cut(s) 341, 430
Cfr13I GGNCC 3 cut(s) 310, 686, 687
Cfr9I CCCGGG 1 cut(s) 670
Csp6I GTAC 5 cut(s) 207, 246, 408, 643, 767
CviAII CATG 5 cut(s) 55, 79, 296, 336, 344
CviQI GTAC 5 cut(s) 207, 246, 408, 643, 767
DdeI CTNAG 4 cut(s) 267, 319, 433, 876
DpnI GATC 2 cut(s) 17, 798
DpnII GATC 2 cut(s) 15, 796
DraIII CACNNNGTG 1 cut(s) 956
Eco130I CCWWGG 2 cut(s) 54, 830
Eco24I GRGCYC 1 cut(s) 690
Eco88I CYCGRG 1 cut(s) 670
EcoO109I RGGNCCY 2 cut(s) 686, 687
EcoRII CCWGG 2 cut(s) 511, 976
EcoT14I CCWWGG 2 cut(s) 54, 830
EcoT38I GRGCYC 1 cut(s) 690
ErhI CCWWGG 2 cut(s) 54, 830
FaeI CATG 5 cut(s) 58, 82, 299, 339, 347
FaqI GGGAC 1 cut(s) 530
FatI CATG 5 cut(s) 54, 78, 295, 335, 343
Fnu4HI GCNGC 1 cut(s) 591
FokI GGATG 3 cut(s) 411, 437, 716
FriOI GRGCYC 1 cut(s) 690
Fsp4HI GCNGC 1 cut(s) 591
FspAI RTGCGCAY 1 cut(s) 429
FspBI CTAG 2 cut(s) 306, 582
FspI TGCGCA 1 cut(s) 429
GlaI GCGC 2 cut(s) 340, 429
GluI GCNGC 1 cut(s) 591
HaeIII GGCC 4 cut(s) 238, 312, 688, 829
HapII CCGG 5 cut(s) 188, 228, 239, 671, 954
HhaI GCGC 2 cut(s) 341, 430
Hin1II CATG 5 cut(s) 58, 82, 299, 339, 347
Hin6I GCGC 2 cut(s) 339, 428
HinP1I GCGC 2 cut(s) 339, 428
HinfI GANTC 8 cut(s) 39, 74, 112, 374, 562, 578, 776, 947
HpaII CCGG 5 cut(s) 188, 228, 239, 671, 954
HphI GGTGA 4 cut(s) 427, 942, 968, 992
Hpy166II GTNNAC 2 cut(s) 97, 535
Hpy188I TCNGA 4 cut(s) 398, 436, 561, 994
Hpy188III TCNNGA 8 cut(s) 167, 321, 331, 575, 638, 681, 805, 944
Hpy8I GTNNAC 2 cut(s) 97, 535
HpyAV CCTTC 1 cut(s) 113
HpyCH4III ACNGT 4 cut(s) 412, 521, 544, 605
HpyCH4IV ACGT 1 cut(s) 984
HpyCH4V TGCA 3 cut(s) 260, 289, 899
HpyF10VI GCNNNNNNNGC 1 cut(s) 224
HpyF3I CTNAG 4 cut(s) 267, 319, 433, 876
HpySE526I ACGT 1 cut(s) 984
Hsp92II CATG 5 cut(s) 58, 82, 299, 339, 347
HspAI GCGC 2 cut(s) 339, 428
Kzo9I GATC 2 cut(s) 15, 796
LmnI GCTCC 2 cut(s) 241, 304
Lsp1109I GCAGC 1 cut(s) 577
LweI GCATC 1 cut(s) 415
MaeI CTAG 2 cut(s) 306, 582
MaeII ACGT 1 cut(s) 984
MaeIII GTNAC 3 cut(s) 937, 948, 980
MalI GATC 2 cut(s) 17, 798
MboI GATC 2 cut(s) 15, 796
MboII GAAGA 2 cut(s) 375, 400
MfeI CAATTG 1 cut(s) 771
MflI RGATCY 1 cut(s) 15
MhlI GDGCHC 2 cut(s) 220, 690
MluCI AATT 4 cut(s) 457, 676, 771, 995
MlyI GAGTC 1 cut(s) 956
MmeI TCCRAC 2 cut(s) 326, 376
MnlI CCTC 9 cut(s) 28, 192, 228, 376, 397, 677, 810, 877, 884
MseI TTAA 4 cut(s) 359, 723, 843, 858
MslI CAYNNNNRTG 2 cut(s) 294, 542
MspI CCGG 5 cut(s) 188, 228, 239, 671, 954
MspR9I CCNGG 6 cut(s) 229, 513, 671, 672, 954, 978
MunI CAATTG 1 cut(s) 771
MvaI CCWGG 2 cut(s) 513, 978
MwoI GCNNNNNNNGC 1 cut(s) 224
NciI CCSGG 4 cut(s) 229, 671, 672, 954
NcoI CCATGG 1 cut(s) 54
NdeII GATC 2 cut(s) 15, 796
NlaIII CATG 5 cut(s) 58, 82, 299, 339, 347
NlaIV GGNNCC 6 cut(s) 180, 217, 311, 688, 689, 971
NmuCI GTSAC 2 cut(s) 948, 980
NsbI TGCGCA 1 cut(s) 429
NspI RCATGY 1 cut(s) 339
NspV TTCGAA 1 cut(s) 129
PcsI WCGNNNNNNNCGW 1 cut(s) 990
PfeI GAWTC 7 cut(s) 39, 74, 112, 374, 562, 578, 776
PflFI GACNNNGTC 2 cut(s) 521, 986
PflMI CCANNNNNTGG 1 cut(s) 70
PkrI GCNGC 1 cut(s) 592
PleI GAGTC 1 cut(s) 955
PpsI GAGTC 1 cut(s) 955
Psp6I CCWGG 2 cut(s) 511, 976
PspGI CCWGG 2 cut(s) 511, 976
PspN4I GGNNCC 6 cut(s) 180, 217, 311, 688, 689, 971
PspOMI GGGCCC 1 cut(s) 686
PspPI GGNCC 3 cut(s) 310, 686, 687
PsuI RGATCY 1 cut(s) 15
PsyI GACNNNGTC 2 cut(s) 521, 986
RsaI GTAC 5 cut(s) 208, 247, 409, 644, 768
RsaNI GTAC 5 cut(s) 207, 246, 408, 643, 767
RseI CAYNNNNRTG 2 cut(s) 294, 542
SaqAI TTAA 4 cut(s) 359, 723, 843, 858
SatI GCNGC 1 cut(s) 591
Sau3AI GATC 2 cut(s) 15, 796
Sau96I GGNCC 3 cut(s) 310, 686, 687
SchI GAGTC 1 cut(s) 956
ScrFI CCNGG 6 cut(s) 229, 513, 671, 672, 954, 978
SduI GDGCHC 2 cut(s) 220, 690
SetI ASST 6 cut(s) 619, 745, 858, 882, 895, 987
SfaNI GCATC 1 cut(s) 415
SfuI TTCGAA 1 cut(s) 129
SmaI CCCGGG 1 cut(s) 672
SmiMI CAYNNNNRTG 2 cut(s) 294, 542
SmlI CTYRAG 2 cut(s) 881, 944
SmoI CTYRAG 2 cut(s) 881, 944
Sse9I AATT 4 cut(s) 457, 676, 771, 995
SsiI CCGC 1 cut(s) 530
SspI AATATT 1 cut(s) 924
SspMI CTAG 2 cut(s) 306, 582
StyD4I CCNGG 6 cut(s) 227, 511, 669, 670, 952, 976
StyI CCWWGG 2 cut(s) 54, 830
TaaI ACNGT 4 cut(s) 412, 521, 544, 605
TaiI ACGT 1 cut(s) 987
TaqI TCGA 2 cut(s) 129, 637
TasI AATT 4 cut(s) 457, 676, 771, 995
TatI WGTACW 1 cut(s) 642
TfiI GAWTC 7 cut(s) 39, 74, 112, 374, 562, 578, 776
Tru1I TTAA 4 cut(s) 359, 723, 843, 858
Tru9I TTAA 4 cut(s) 359, 723, 843, 858
TscAI CASTG 3 cut(s) 262, 477, 610
TseFI GTSAC 2 cut(s) 948, 980
TseI GCWGC 1 cut(s) 590
Tsp45I GTSAC 2 cut(s) 948, 980
TspDTI ATGAA 3 cut(s) 95, 112, 284
TspGWI ACGGA 1 cut(s) 962
TspMI CCCGGG 1 cut(s) 670
TspRI CASTG 3 cut(s) 262, 477, 610
Tth111I GACNNNGTC 2 cut(s) 521, 986
Van91I CCANNNNNTGG 1 cut(s) 70
XapI RAATTY 2 cut(s) 676, 995
XceI RCATGY 1 cut(s) 339
XmaI CCCGGG 1 cut(s) 670
XspI CTAG 2 cut(s) 306, 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.