FvH4_6g06410

protease Do-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005022
Physical Location & Seq
Reverse (-)
3737630 .. 3741340
3711 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g06410.t1

Sequence Viewer

Length: 1260 bp
ATGATCCATTCTCTGAGGAAGCTGCTTTCAACAAAGGACCGCATTCTGGCCGTGGCTGCCGCCGGGTCGGCTATGTTGTACGCCACCAGCACCGGAAGATCCAAATGGACTGTAGTGGGATCGATTCCTACAACAATAAGGGAATCATTGCTGCTTCCATGGAAACCAAAGCAATGGGATCAATGCCCCTTTGGAATGCTTCCAATGTATTGTTTTAGTGATGTTTTGGTGGAGGATGGATCGGTGTCTCAACTGGACACAAAGAAAGAAGATGCTTCTGGTGTCACTATAGCCGGGGAGAGGCCGGAGCGTACTTATGCCACTGCATTTGCTAAGGTTGGCCCTGCTGTGGTTCATATTCATGCTCCTAGTAGGGGTCATTGCGCTGGAACAATCATAGATAAGGACGGGATTGTCTTGACATGCGCTCATGCTTTTGTTGATTTAGGATTAGGAACACTTGTAGATTCGTATGAGTGCAAGGTTTATATAACCTTGCAAGATGGCCGGAGACTTGAGGGTACTGTGGTGAATTTGGATGTGCATTCGGACATTGCTATTATCAAAGTCGATTCTGACACCCCATTGCCATCAGCAGAACTTGGTTGCTCAAGTAAAGTCCAACCAGGGGAGTGTGTCATAGCTGTTGGATCTCCACTTTCATTTAAACATACTGTCACAGCTGGTATTGTAAGTTGTGCTGCTCGTAAAAACAGCGAAATGAGACAAGATGGGTGGAGAGAGTATAAAGAGTATATACAGACAGATTGCTCAATCAACCCGGGAAATTCAGGAGGGCCCTTAGTAAATATGGACGGAGAAGTTATTGCTATTAATGTTAGGATGATACGAGCTGCTGATGGTTTGAATTTTGCCGTACCAATTGACTCAGTCACTAAAGTTATGGATCAGTTTAAGAAAAATGGGAGGGTTGTCCGGCCTTGGCTTGGAGTTAAAATGATTGAGCTTAATGATATGATAATCTCTCAGCTTGAGGAAAGAGGTTCTGCATTTCCAAATGTAAAGAAAGGAATCCTTGTGCCTATGGTAACTCCTAGGTCACCTGGTGAGCGTGCTGGGTTCCGTCCTGGTGATGTTGTCTCCAAATTTGATGGAAGGACTGTTAAAACCGTTAAAGAGATCATTGAAATAGTCGGAGACAGAGTGGGAGAACCCTTAAAAGTTGTTGTGAAAAGAGTGAACGATGAGCAGGTGATACTGACTGTGATCCCAGAGGAGTCATCTGATCCGGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04210 ko04214 ko04215 ko05012 map04210 map04214 map04215 map05012
Pfam Domains
Protein Families
PDZ_2 Trypsin_2

Protein Analysis

420

Amino Acids

45.46

Weight (kDa)

6.42

Isoelectric Point (pI)

33.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trypsin PF00089 114 - 300 1.7e-16 Trypsin
Trypsin_2 PF13365 128 - 278 1.4e-29 Trypsin-like peptidase domain
PDZ_2 PF13180 316 - 404 3.3e-09 PDZ domain
PDZ_6 PF17820 350 - 399 2.3e-09 PDZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1201
AasI GACNNNNNNGTC 1 cut(s) 413
Acc36I ACCTGC 1 cut(s) 1201
AccIII TCCGGA 1 cut(s) 1249
AciI CCGC 2 cut(s) 40, 60
AclWI GGATC 8 cut(s) 93, 127, 186, 247, 658, 915, 1222, 1241
AcoI YGGCCR 2 cut(s) 48, 505
AcsI RAATTY 4 cut(s) 532, 787, 868, 1106
AdeI CACNNNGTG 1 cut(s) 1067
AfaI GTAC 4 cut(s) 80, 313, 523, 879
AfiI CCNNNNNNNGG 6 cut(s) 46, 300, 349, 374, 628, 947
AgsI TTSAA 3 cut(s) 30, 868, 1148
AjnI CCWGG 3 cut(s) 625, 1063, 1087
AluBI AGCT 6 cut(s) 22, 644, 683, 854, 967, 991
AluI AGCT 6 cut(s) 22, 644, 683, 854, 967, 991
Alw26I GTCTC 5 cut(s) 252, 505, 718, 1105, 1152
AlwI GGATC 8 cut(s) 93, 127, 186, 247, 658, 915, 1222, 1241
Ama87I CYCGRG 1 cut(s) 781
Aor13HI TCCGGA 1 cut(s) 1249
AoxI GGCC 6 cut(s) 48, 302, 340, 505, 797, 938
ApaI GGGCCC 1 cut(s) 801
ApeKI GCWGC 5 cut(s) 22, 56, 151, 701, 854
ApoI RAATTY 4 cut(s) 532, 787, 868, 1106
AseI ATTAAT 1 cut(s) 834
AspA2I CCTAGG 1 cut(s) 1055
AspLEI GCGC 2 cut(s) 386, 428
AspS9I GGNCC 4 cut(s) 37, 341, 797, 798
AsuC2I CCSGG 4 cut(s) 64, 295, 782, 783
AsuHPI GGTGA 5 cut(s) 541, 1053, 1079, 1103, 1225
AvaI CYCGRG 1 cut(s) 781
AvaII GGWCC 1 cut(s) 37
AvrII CCTAGG 1 cut(s) 1055
BaeGI GKGCMC 1 cut(s) 801
BaeI ACNNNNGTAYC 2 cut(s) 513, 546
BanII GRGCYC 1 cut(s) 801
BbvI GCAGC 5 cut(s) 9, 43, 138, 688, 841
BccI CCATC 6 cut(s) 230, 497, 598, 725, 854, 1106
BceAI ACGGC 2 cut(s) 35, 860
BciT130I CCWGG 3 cut(s) 627, 1065, 1089
BcnI CCSGG 4 cut(s) 64, 295, 782, 783
BcoDI GTCTC 5 cut(s) 252, 505, 718, 1105, 1152
BfaI CTAG 2 cut(s) 369, 1056
BfmI CTRYAG 2 cut(s) 111, 288
BfuAI ACCTGC 1 cut(s) 1201
BglI GCCNNNNNGGC 1 cut(s) 68
BisI GCNGC 6 cut(s) 23, 57, 60, 152, 702, 855
BlnI CCTAGG 1 cut(s) 1055
BlsI GCNGC 6 cut(s) 24, 58, 61, 153, 703, 856
Bme1390I CCNGG 7 cut(s) 64, 295, 627, 782, 783, 1065, 1089
Bme18I GGWCC 1 cut(s) 37
BmeT110I CYCGRG 1 cut(s) 781
BmgT120I GGNCC 4 cut(s) 37, 341, 797, 798
BmiI GGNNCC 2 cut(s) 799, 1082
BmrFI CCNGG 7 cut(s) 64, 295, 627, 782, 783, 1065, 1089
BmsI GCATC 1 cut(s) 262
Bpu10I CCTNAGC 1 cut(s) 333
BpuEI CTTGAG 3 cut(s) 536, 595, 1013
BpuMI CCSGG 4 cut(s) 64, 295, 782, 783
Bsa29I ATCGAT 1 cut(s) 122
BsaJI CCNNGG 7 cut(s) 51, 158, 294, 626, 781, 941, 1055
BsaWI WCCGGW 2 cut(s) 92, 1249
BsaXI ACNNNNNCTCC 2 cut(s) 1149, 1179
Bsc4I CCNNNNNNNGG 6 cut(s) 46, 300, 349, 374, 628, 947
Bse1I ACTGG 1 cut(s) 258
Bse3DI GCAATG 5 cut(s) 146, 179, 379, 552, 584
BseAI TCCGGA 1 cut(s) 1249
BseBI CCWGG 3 cut(s) 627, 1065, 1089
BseCI ATCGAT 1 cut(s) 122
BseDI CCNNGG 7 cut(s) 51, 158, 294, 626, 781, 941, 1055
BseGI GGATG 3 cut(s) 241, 544, 849
BseLI CCNNNNNNNGG 6 cut(s) 46, 300, 349, 374, 628, 947
BseMI GCAATG 5 cut(s) 146, 179, 379, 552, 584
BseMII CTCAG 3 cut(s) 5, 903, 1001
BseNI ACTGG 1 cut(s) 258
BseRI GAGGAG 1 cut(s) 1250
BseSI GKGCMC 1 cut(s) 801
BseXI GCAGC 5 cut(s) 9, 43, 138, 688, 841
BseYI CCCAGC 1 cut(s) 1076
BshFI GGCC 6 cut(s) 50, 304, 342, 507, 799, 940
BshVI ATCGAT 1 cut(s) 122
BsiHKCI CYCGRG 1 cut(s) 781
BsiSI CCGG 8 cut(s) 63, 93, 294, 305, 508, 782, 937, 1250
BslI CCNNNNNNNGG 6 cut(s) 46, 300, 349, 374, 628, 947
BsmAI GTCTC 5 cut(s) 252, 505, 718, 1105, 1152
BsmI GAATGC 3 cut(s) 42, 201, 544
BsnI GGCC 6 cut(s) 50, 304, 342, 507, 799, 940
BsoBI CYCGRG 1 cut(s) 781
Bsp120I GGGCCC 1 cut(s) 797
Bsp1286I GDGCHC 1 cut(s) 801
Bsp13I TCCGGA 1 cut(s) 1249
Bsp19I CCATGG 1 cut(s) 158
BspACI CCGC 2 cut(s) 40, 60
BspANI GGCC 6 cut(s) 50, 304, 342, 507, 799, 940
BspCNI CTCAG 3 cut(s) 6, 902, 1000
BspDI ATCGAT 1 cut(s) 122
BspEI TCCGGA 1 cut(s) 1249
BspLI GGNNCC 2 cut(s) 799, 1082
BspMI ACCTGC 1 cut(s) 1201
BspPI GGATC 8 cut(s) 93, 127, 186, 247, 658, 915, 1222, 1241
BsrDI GCAATG 5 cut(s) 146, 179, 379, 552, 584
BsrI ACTGG 1 cut(s) 258
BssECI CCNNGG 7 cut(s) 51, 158, 294, 626, 781, 941, 1055
BssT1I CCWWGG 3 cut(s) 158, 941, 1055
Bst2UI CCWGG 3 cut(s) 627, 1065, 1089
Bst4CI ACNGT 6 cut(s) 112, 526, 676, 1123, 1132, 1225
BstC8I GCNNGC 1 cut(s) 1074
BstDEI CTNAG 5 cut(s) 14, 333, 802, 889, 987
BstDSI CCRYGG 2 cut(s) 51, 158
BstEII GGTNACC 1 cut(s) 1059
BstF5I GGATG 3 cut(s) 241, 544, 849
BstHHI GCGC 2 cut(s) 386, 428
BstMAI GTCTC 5 cut(s) 252, 505, 718, 1105, 1152
BstMWI GCNNNNNNNGC 2 cut(s) 56, 68
BstNI CCWGG 3 cut(s) 627, 1065, 1089
BstNSI RCATGY 1 cut(s) 426
BstPI GGTNACC 1 cut(s) 1059
BstSCI CCNGG 7 cut(s) 62, 293, 625, 780, 781, 1063, 1087
BstSFI CTRYAG 2 cut(s) 111, 288
BstSLI GKGCMC 1 cut(s) 801
BstV1I GCAGC 5 cut(s) 9, 43, 138, 688, 841
BstX2I RGATCY 2 cut(s) 98, 650
BstXI CCANNNNNNTGG 1 cut(s) 174
BstYI RGATCY 2 cut(s) 98, 650
Bsu15I ATCGAT 1 cut(s) 122
BsuRI GGCC 6 cut(s) 50, 304, 342, 507, 799, 940
BsuTUI ATCGAT 1 cut(s) 122
BtgI CCRYGG 2 cut(s) 51, 158
BtsCI GGATG 3 cut(s) 241, 544, 849
BtsI GCAGTG 1 cut(s) 321
BtsIMutI CAGTG 1 cut(s) 321
BveI ACCTGC 1 cut(s) 1201
Cac8I GCNNGC 1 cut(s) 1074
CfoI GCGC 2 cut(s) 386, 428
Cfr13I GGNCC 4 cut(s) 37, 341, 797, 798
Cfr9I CCCGGG 1 cut(s) 781
ClaI ATCGAT 1 cut(s) 122
CsiI ACCWGGT 1 cut(s) 1063
Csp6I GTAC 4 cut(s) 79, 312, 522, 878
CspCI CAANNNNNGTGG 4 cut(s) 310, 345, 716, 751
CviAII CATG 4 cut(s) 159, 362, 423, 431
CviQI GTAC 4 cut(s) 79, 312, 522, 878
DdeI CTNAG 5 cut(s) 14, 333, 802, 889, 987
DraI TTTAAA 1 cut(s) 667
DraIII CACNNNGTG 1 cut(s) 1067
DrdI GACNNNNNNGTC 1 cut(s) 413
DseDI GACNNNNNNGTC 1 cut(s) 413
EaeI YGGCCR 2 cut(s) 48, 505
Eco130I CCWWGG 3 cut(s) 158, 941, 1055
Eco24I GRGCYC 1 cut(s) 801
Eco47I GGWCC 1 cut(s) 37
Eco88I CYCGRG 1 cut(s) 781
Eco91I GGTNACC 1 cut(s) 1059
EcoO109I RGGNCCY 2 cut(s) 797, 798
EcoO65I GGTNACC 1 cut(s) 1059
EcoRII CCWGG 3 cut(s) 625, 1063, 1087
EcoT14I CCWWGG 3 cut(s) 158, 941, 1055
EcoT38I GRGCYC 1 cut(s) 801
ErhI CCWWGG 3 cut(s) 158, 941, 1055
FaeI CATG 4 cut(s) 162, 365, 426, 434
FalI AAGNNNNNCTT 2 cut(s) 1020, 1052
FatI CATG 4 cut(s) 158, 361, 422, 430
Fnu4HI GCNGC 6 cut(s) 23, 57, 60, 152, 702, 855
FokI GGATG 3 cut(s) 248, 551, 856
FriOI GRGCYC 1 cut(s) 801
Fsp4HI GCNGC 6 cut(s) 23, 57, 60, 152, 702, 855
FspBI CTAG 2 cut(s) 369, 1056
GlaI GCGC 2 cut(s) 385, 427
GluI GCNGC 6 cut(s) 23, 57, 60, 152, 702, 855
GsaI CCCAGC 1 cut(s) 1080
HaeIII GGCC 6 cut(s) 50, 304, 342, 507, 799, 940
HapII CCGG 8 cut(s) 63, 93, 294, 305, 508, 782, 937, 1250
HhaI GCGC 2 cut(s) 386, 428
Hin1II CATG 4 cut(s) 162, 365, 426, 434
Hin6I GCGC 2 cut(s) 384, 426
HinP1I GCGC 2 cut(s) 384, 426
HinfI GANTC 7 cut(s) 124, 143, 467, 572, 887, 1032, 1238
HpaII CCGG 8 cut(s) 63, 93, 294, 305, 508, 782, 937, 1250
HphI GGTGA 5 cut(s) 541, 1053, 1079, 1103, 1225
Hpy166II GTNNAC 1 cut(s) 1201
Hpy188I TCNGA 5 cut(s) 15, 550, 577, 1157, 1246
Hpy188III TCNNGA 3 cut(s) 418, 792, 1250
Hpy8I GTNNAC 1 cut(s) 1201
HpyAV CCTTC 1 cut(s) 1110
HpyCH4III ACNGT 6 cut(s) 112, 526, 676, 1123, 1132, 1225
HpyCH4V TGCA 5 cut(s) 326, 480, 499, 544, 1010
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 68
HpyF3I CTNAG 5 cut(s) 14, 333, 802, 889, 987
Hsp92II CATG 4 cut(s) 162, 365, 426, 434
HspAI GCGC 2 cut(s) 384, 426
Kpn2I TCCGGA 1 cut(s) 1249
LmnI GCTCC 2 cut(s) 307, 370
Lsp1109I GCAGC 5 cut(s) 9, 43, 138, 688, 841
LweI GCATC 1 cut(s) 262
MabI ACCWGGT 1 cut(s) 1063
MaeI CTAG 2 cut(s) 369, 1056
MaeIII GTNAC 5 cut(s) 283, 676, 892, 1048, 1059
MboII GAAGA 2 cut(s) 108, 281
MfeI CAATTG 1 cut(s) 882
MflI RGATCY 2 cut(s) 98, 650
MhlI GDGCHC 1 cut(s) 801
MluCI AATT 5 cut(s) 532, 787, 868, 882, 1106
MlyI GAGTC 2 cut(s) 881, 1247
MmeI TCCRAC 3 cut(s) 628, 646, 1135
MnlI CCTC 9 cut(s) 9, 226, 294, 511, 788, 921, 988, 995, 1228
MroI TCCGGA 1 cut(s) 1249
MseI TTAA 8 cut(s) 666, 834, 915, 954, 969, 1125, 1134, 1178
MslI CAYNNNNRTG 1 cut(s) 360
MspA1I CMGCKG 1 cut(s) 683
MspI CCGG 8 cut(s) 63, 93, 294, 305, 508, 782, 937, 1250
MspR9I CCNGG 7 cut(s) 64, 295, 627, 782, 783, 1065, 1089
MunI CAATTG 1 cut(s) 882
Mva1269I GAATGC 3 cut(s) 42, 201, 544
MvaI CCWGG 3 cut(s) 627, 1065, 1089
MwoI GCNNNNNNNGC 2 cut(s) 56, 68
NciI CCSGG 4 cut(s) 64, 295, 782, 783
NcoI CCATGG 1 cut(s) 158
NlaIII CATG 4 cut(s) 162, 365, 426, 434
NlaIV GGNNCC 2 cut(s) 799, 1082
NmuCI GTSAC 4 cut(s) 283, 676, 892, 1059
NspI RCATGY 1 cut(s) 426
PaqCI CACCTGC 1 cut(s) 1201
PctI GAATGC 3 cut(s) 42, 201, 544
PfeI GAWTC 5 cut(s) 124, 143, 467, 572, 1032
PflFI GACNNNGTC 1 cut(s) 890
PkrI GCNGC 6 cut(s) 24, 58, 61, 153, 703, 856
PleI GAGTC 2 cut(s) 881, 1246
PpsI GAGTC 2 cut(s) 881, 1246
PshBI ATTAAT 1 cut(s) 834
Psp6I CCWGG 3 cut(s) 625, 1063, 1087
PspEI GGTNACC 1 cut(s) 1059
PspFI CCCAGC 1 cut(s) 1076
PspGI CCWGG 3 cut(s) 625, 1063, 1087
PspN4I GGNNCC 2 cut(s) 799, 1082
PspOMI GGGCCC 1 cut(s) 797
PspPI GGNCC 4 cut(s) 37, 341, 797, 798
PsuI RGATCY 2 cut(s) 98, 650
PsyI GACNNNGTC 1 cut(s) 890
PvuII CAGCTG 1 cut(s) 683
RsaI GTAC 4 cut(s) 80, 313, 523, 879
RsaNI GTAC 4 cut(s) 79, 312, 522, 878
RseI CAYNNNNRTG 1 cut(s) 360
SaqAI TTAA 8 cut(s) 666, 834, 915, 954, 969, 1125, 1134, 1178
SatI GCNGC 6 cut(s) 23, 57, 60, 152, 702, 855
Sau96I GGNCC 4 cut(s) 37, 341, 797, 798
SchI GAGTC 2 cut(s) 881, 1247
ScrFI CCNGG 7 cut(s) 64, 295, 627, 782, 783, 1065, 1089
SduI GDGCHC 1 cut(s) 801
SexAI ACCWGGT 1 cut(s) 1063
SfaNI GCATC 1 cut(s) 262
SfcI CTRYAG 2 cut(s) 111, 288
SinI GGWCC 1 cut(s) 37
SmaI CCCGGG 1 cut(s) 783
SmiMI CAYNNNNRTG 1 cut(s) 360
SmlI CTYRAG 3 cut(s) 515, 610, 992
SmoI CTYRAG 3 cut(s) 515, 610, 992
Sse9I AATT 5 cut(s) 532, 787, 868, 882, 1106
SsiI CCGC 2 cut(s) 40, 60
SspMI CTAG 2 cut(s) 369, 1056
StyD4I CCNGG 7 cut(s) 62, 293, 625, 780, 781, 1063, 1087
StyI CCWWGG 3 cut(s) 158, 941, 1055
TaaI ACNGT 6 cut(s) 112, 526, 676, 1123, 1132, 1225
TaqI TCGA 2 cut(s) 122, 570
TasI AATT 5 cut(s) 532, 787, 868, 882, 1106
TauI GCSGC 1 cut(s) 62
TfiI GAWTC 5 cut(s) 124, 143, 467, 572, 1032
Tru1I TTAA 8 cut(s) 666, 834, 915, 954, 969, 1125, 1134, 1178
Tru9I TTAA 8 cut(s) 666, 834, 915, 954, 969, 1125, 1134, 1178
TscAI CASTG 1 cut(s) 328
TseFI GTSAC 4 cut(s) 283, 676, 892, 1059
TseI GCWGC 5 cut(s) 22, 56, 151, 701, 854
Tsp45I GTSAC 4 cut(s) 283, 676, 892, 1059
TspDTI ATGAA 3 cut(s) 344, 350, 651
TspGWI ACGGA 2 cut(s) 831, 1073
TspMI CCCGGG 1 cut(s) 781
TspRI CASTG 1 cut(s) 328
Tth111I GACNNNGTC 1 cut(s) 890
VpaK11BI GGWCC 1 cut(s) 37
VspI ATTAAT 1 cut(s) 834
XapI RAATTY 4 cut(s) 532, 787, 868, 1106
XceI RCATGY 1 cut(s) 426
XmaI CCCGGG 1 cut(s) 781
XmaJI CCTAGG 1 cut(s) 1055
XspI CTAG 2 cut(s) 369, 1056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.