Rorug03G0007900

protease Do-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005022
Physical Location & Seq
Reverse (-)
620226 .. 623481
3256 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0007900.1

Sequence Viewer

Length: 1476 bp
ATGGCAGACTCAGATTATCGTTCTCCAATAGTACTCTCTGTAACTCTTCTCCTCCTCTTCGTTACAGTCCTCTTCTTCTCTCCATTAAACCGAAACCAATATCTTTCGCTCAATCTTTTATCTCCTTCCTCCTCCCTATCTAACTCTTCTTCTACTCATCATAACCAACAAACCACCGACAACGACAAAGCCGTGCATGTCGTTTCAGCACCGGCACCGGCGCCGTCGCTTCCGCCGTTTCACCAACATGGGCCTCCCTCCACTCTCACTTTGCCTCAAGTACTCAGTCACAGTGTCAAAAACAAGAGCAAAACGCAAAGGATTGAAGAAGAGTTGGCCAGAGCAAGAGCCATTATTCGCAAAGCTATTCTGACGAAGAATTACACCTCTGACAGGAATGAGATTTACATCCCAAGAGGAAGTGTTTACAGAAATCCCTATGCATTTCATCAGAGTCATATAGAAATGGTCAAGACATTCAAGGTCTGGGCCTATAGGGAAGGAGAAGTACCAATGGTCCATGATGGGCCAACTACGTACCTCTACTCCATAGAGGGCCAGTTCATTTTTGAGATGGAAAGTCGGCCAAGCCCTTTCATGGCCAAGAATCCTGATGAGGCTCATACATTCTTCTTACCATTGAGTGTGTCCAAGATTACTGACTTCCTGTTCAGACCTCACCATGAGACATTTTTTCATCGACTGGGTCGCGTCTTCACCGACTACATTCATGTTGTACAAGAAAAGTATCCTTACTGGAATAGGAGTGGTGGAGCAGACCATTTTATGGTCTCATGTCATGATTGGGCATCACTTATCCAACGTGATGACCCCAAGCCGTACAAGAATCTGATGAAGGTGTTATGCAATGCAAACATCTCTGAAGGGTTTAAGCCTACTAGAGATGTCTCAATACCAGAATACAACTTAAAACGCTACGAGCTTGGCCCTCCCCGTTTTGGGCTACCCCCTCATAACCGTACTGTCCTTGCTTTCTTTGCTGGTGCAGCCCATGGAGACATAAGGAGCATTTTGTTTGAGCATTGGAAGGAAAAAGATGATGAAGTTCGGGTCTATGAAAAACTTCCTCAGACTATAAACTACCACAAGATCATGGGACAAACCAAGTTTTGCTTGTGCCCGAGTGGTTCTGAAGTGGCAAGTCCTAGAGTAGTGGAGGCAATGAATGCAGGGTGTGTCCCGGTGCTAATTTCCGATTCCTATGCAATACCTTTTGCTGATGTTCTTAACTGGAACAAGTTTTCTATACAAATTCCGCCCAAGAGAATATCAGAAATCAAGACAATATTGAAGGCTGTTCCACACAAAAGGTACTTGACATTGCAGAAGAGGGTGATGCAAGTGAGAAGGCATTTTGAGCTCAACCGACCAGCTAAGCCATTTGATGTATTCCATATGGTGCTTCACTCAGTGTGGCTTAAAAGGCTCAACATTCGATTACCTGATAAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04210 ko04214 ko04215 ko05012 map04210 map04214 map04215 map05012
Pfam Domains
Protein Families
PDZ_2 Trypsin_2

Protein Analysis

491

Amino Acids

56.6

Weight (kDa)

9.47

Isoelectric Point (pI)

48.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 142 - 439 1.2e-56 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 214, 220
AccB7I CCANNNNNTGG 1 cut(s) 787
AccII CGCG 1 cut(s) 711
AciI CCGC 2 cut(s) 233, 1277
AcoI YGGCCR 3 cut(s) 336, 584, 600
AcsI RAATTY 2 cut(s) 1272, 1469
AcuI CTGAAG 2 cut(s) 903, 1173
AcyI GRCGYC 1 cut(s) 221
AdeI CACNNNGTG 1 cut(s) 1432
AfaI GTAC 8 cut(s) 33, 282, 510, 539, 738, 842, 982, 1334
AfiI CCNNNNNNNGG 5 cut(s) 393, 598, 787, 959, 960
AgsI TTSAA 3 cut(s) 326, 481, 1312
AluBI AGCT 4 cut(s) 365, 943, 1381, 1394
AluI AGCT 4 cut(s) 365, 943, 1381, 1394
Alw21I GWGCWC 1 cut(s) 1383
Alw26I GTCTC 4 cut(s) 680, 796, 913, 1011
Ama87I CYCGRG 1 cut(s) 1141
AoxI GGCC 8 cut(s) 251, 336, 489, 527, 556, 584, 600, 946
ApeKI GCWGC 1 cut(s) 1007
ApoI RAATTY 2 cut(s) 1272, 1469
Asp700I GAANNNNTTC 1 cut(s) 1083
AspLEI GCGC 1 cut(s) 223
AspS9I GGNCC 6 cut(s) 251, 489, 517, 527, 556, 947
AsuC2I CCSGG 1 cut(s) 1202
AsuHPI GGTGA 4 cut(s) 233, 671, 709, 1366
AvaI CYCGRG 1 cut(s) 1141
AvaII GGWCC 1 cut(s) 517
BaeGI GKGCMC 1 cut(s) 1142
BalI TGGCCA 2 cut(s) 338, 602
BanI GGYRCC 2 cut(s) 214, 220
BanII GRGCYC 1 cut(s) 1383
BarI GAAGNNNNNNTAC 2 cut(s) 492, 524
BbsI GAAGAC 1 cut(s) 706
Bbv12I GWGCWC 1 cut(s) 1383
BbvI GCAGC 1 cut(s) 1019
BccI CCATC 2 cut(s) 518, 568
BceAI ACGGC 4 cut(s) 176, 208, 220, 823
BcgI CGANNNNNNTGC 2 cut(s) 1205, 1239
BciVI GTATCC 1 cut(s) 759
BcnI CCSGG 1 cut(s) 1202
BcoDI GTCTC 4 cut(s) 680, 796, 913, 1011
BfaI CTAG 2 cut(s) 900, 1167
BfmI CTRYAG 1 cut(s) 493
BfoI RGCGCY 1 cut(s) 224
BfuI GTATCC 1 cut(s) 759
BisI GCNGC 1 cut(s) 1008
BlpI GCTNAGC 1 cut(s) 1395
BlsI GCNGC 1 cut(s) 1009
BmcAI AGTACT 2 cut(s) 33, 282
Bme1390I CCNGG 1 cut(s) 1202
Bme18I GGWCC 1 cut(s) 517
BmeT110I CYCGRG 1 cut(s) 1141
BmgT120I GGNCC 6 cut(s) 251, 489, 517, 527, 556, 947
BmiI GGNNCC 2 cut(s) 216, 222
BmrFI CCNGG 1 cut(s) 1202
BmrI ACTGGG 1 cut(s) 713
BmsI GCATC 2 cut(s) 818, 1347
BmuI ACTGGG 1 cut(s) 713
BpiI GAAGAC 1 cut(s) 706
Bpu1102I GCTNAGC 1 cut(s) 1395
BpuEI CTTGAG 1 cut(s) 261
BpuMI CCSGG 1 cut(s) 1202
BsaAI YACGTR 1 cut(s) 537
BsaBI GATNNNNATC 1 cut(s) 407
BsaHI GRCGYC 1 cut(s) 221
BsaI GGTCTC 1 cut(s) 796
BsaJI CCNNGG 1 cut(s) 1012
BsaXI ACNNNNNCTCC 2 cut(s) 530, 560
Bsc4I CCNNNNNNNGG 5 cut(s) 393, 598, 787, 959, 960
Bse118I RCCGGY 2 cut(s) 211, 217
Bse1I ACTGG 4 cut(s) 559, 708, 761, 1256
Bse3DI GCAATG 3 cut(s) 874, 1188, 1340
Bse8I GATNNNNATC 1 cut(s) 407
BseDI CCNNGG 1 cut(s) 1012
BseGI GGATG 1 cut(s) 408
BseJI GATNNNNATC 1 cut(s) 407
BseLI CCNNNNNNNGG 5 cut(s) 393, 598, 787, 959, 960
BseMI GCAATG 3 cut(s) 874, 1188, 1340
BseMII CTCAG 4 cut(s) 24, 298, 1103, 1443
BseNI ACTGG 4 cut(s) 559, 708, 761, 1256
BseRI GAGGAG 3 cut(s) 41, 44, 121
BseSI GKGCMC 1 cut(s) 1142
BseXI GCAGC 1 cut(s) 1019
BsgI GTGCAG 1 cut(s) 1026
Bsh1236I CGCG 1 cut(s) 711
BshFI GGCC 8 cut(s) 253, 338, 491, 529, 558, 586, 602, 948
BshNI GGYRCC 2 cut(s) 214, 220
BsiHKAI GWGCWC 1 cut(s) 1383
BsiHKCI CYCGRG 1 cut(s) 1141
BsiSI CCGG 3 cut(s) 212, 218, 1202
BslFI GGGAC 2 cut(s) 1131, 1184
BslI CCNNNNNNNGG 5 cut(s) 393, 598, 787, 959, 960
BsmAI GTCTC 4 cut(s) 680, 796, 913, 1011
BsmFI GGGAC 2 cut(s) 1131, 1184
BsmI GAATGC 1 cut(s) 1192
BsnI GGCC 8 cut(s) 253, 338, 491, 529, 558, 586, 602, 948
Bso31I GGTCTC 1 cut(s) 796
BsoBI CYCGRG 1 cut(s) 1141
Bsp1286I GDGCHC 2 cut(s) 1142, 1383
Bsp1407I TGTACA 1 cut(s) 736
Bsp143I GATC 1 cut(s) 1110
Bsp1720I GCTNAGC 1 cut(s) 1395
Bsp19I CCATGG 1 cut(s) 1012
BspACI CCGC 2 cut(s) 233, 1277
BspANI GGCC 8 cut(s) 253, 338, 491, 529, 558, 586, 602, 948
BspCNI CTCAG 4 cut(s) 23, 297, 1102, 1442
BspFNI CGCG 1 cut(s) 711
BspHI TCATGA 1 cut(s) 799
BspLI GGNNCC 2 cut(s) 216, 222
BspT107I GGYRCC 2 cut(s) 214, 220
BspTNI GGTCTC 1 cut(s) 796
BsrDI GCAATG 3 cut(s) 874, 1188, 1340
BsrFI RCCGGY 2 cut(s) 211, 217
BsrGI TGTACA 1 cut(s) 736
BsrI ACTGG 4 cut(s) 559, 708, 761, 1256
BssAI RCCGGY 2 cut(s) 211, 217
BssECI CCNNGG 1 cut(s) 1012
BssMI GATC 1 cut(s) 1110
BssNI GRCGYC 1 cut(s) 221
BssT1I CCWWGG 1 cut(s) 1012
Bst4CI ACNGT 4 cut(s) 67, 293, 980, 985
Bst6I CTCTTC 6 cut(s) 51, 62, 77, 151, 324, 1343
BstACI GRCGYC 1 cut(s) 221
BstAPI GCANNNNNTGC 1 cut(s) 1187
BstAUI TGTACA 1 cut(s) 736
BstBAI YACGTR 1 cut(s) 537
BstDEI CTNAG 5 cut(s) 10, 284, 1089, 1395, 1429
BstDSI CCRYGG 1 cut(s) 1012
BstENI CCTNNNNNAGG 1 cut(s) 391
BstF5I GGATG 1 cut(s) 408
BstFNI CGCG 1 cut(s) 711
BstH2I RGCGCY 1 cut(s) 224
BstHHI GCGC 1 cut(s) 223
BstKTI GATC 1 cut(s) 1113
BstMAI GTCTC 4 cut(s) 680, 796, 913, 1011
BstMBI GATC 1 cut(s) 1110
BstMWI GCNNNNNNNGC 5 cut(s) 998, 1007, 1187, 1378, 1444
BstNSI RCATGY 1 cut(s) 200
BstSCI CCNGG 1 cut(s) 1200
BstSFI CTRYAG 1 cut(s) 493
BstSLI GKGCMC 1 cut(s) 1142
BstSNI TACGTA 1 cut(s) 537
BstUI CGCG 1 cut(s) 711
BstV1I GCAGC 1 cut(s) 1019
BstV2I GAAGAC 1 cut(s) 706
BsuI GTATCC 1 cut(s) 759
BsuRI GGCC 8 cut(s) 253, 338, 491, 529, 558, 586, 602, 948
BtgI CCRYGG 1 cut(s) 1012
BtsCI GGATG 1 cut(s) 408
BtsIMutI CAGTG 2 cut(s) 298, 1437
CciI TCATGA 1 cut(s) 799
CfoI GCGC 1 cut(s) 223
Cfr10I RCCGGY 2 cut(s) 211, 217
Cfr13I GGNCC 6 cut(s) 251, 489, 517, 527, 556, 947
CseI GACGC 1 cut(s) 700
Csp6I GTAC 8 cut(s) 32, 281, 509, 538, 737, 841, 981, 1333
CviQI GTAC 8 cut(s) 32, 281, 509, 538, 737, 841, 981, 1333
DdeI CTNAG 5 cut(s) 10, 284, 1089, 1395, 1429
DinI GGCGCC 1 cut(s) 222
DpnI GATC 1 cut(s) 1112
DpnII GATC 1 cut(s) 1110
DraIII CACNNNGTG 1 cut(s) 1432
EaeI YGGCCR 3 cut(s) 336, 584, 600
Eam1104I CTCTTC 6 cut(s) 51, 62, 77, 151, 324, 1343
EarI CTCTTC 6 cut(s) 51, 62, 77, 151, 324, 1343
EciI GGCGGA 2 cut(s) 222, 1266
Ecl136II GAGCTC 1 cut(s) 1381
Eco105I TACGTA 1 cut(s) 537
Eco130I CCWWGG 1 cut(s) 1012
Eco24I GRGCYC 1 cut(s) 1383
Eco31I GGTCTC 1 cut(s) 796
Eco47I GGWCC 1 cut(s) 517
Eco53kI GAGCTC 1 cut(s) 1381
Eco57I CTGAAG 2 cut(s) 903, 1173
Eco88I CYCGRG 1 cut(s) 1141
EcoICRI GAGCTC 1 cut(s) 1381
EcoNI CCTNNNNNAGG 1 cut(s) 391
EcoT14I CCWWGG 1 cut(s) 1012
EcoT22I ATGCAT 1 cut(s) 445
EcoT38I GRGCYC 1 cut(s) 1383
EgeI GGCGCC 1 cut(s) 222
EheI GGCGCC 1 cut(s) 222
ErhI CCWWGG 1 cut(s) 1012
FalI AAGNNNNNCTT 2 cut(s) 1118, 1150
FaqI GGGAC 2 cut(s) 1131, 1184
FauNDI CATATG 1 cut(s) 1416
Fnu4HI GCNGC 1 cut(s) 1008
FokI GGATG 1 cut(s) 395
FriOI GRGCYC 1 cut(s) 1383
Fsp4HI GCNGC 1 cut(s) 1008
FspBI CTAG 2 cut(s) 900, 1167
GlaI GCGC 1 cut(s) 222
GluI GCNGC 1 cut(s) 1008
HaeII RGCGCY 1 cut(s) 224
HaeIII GGCC 8 cut(s) 253, 338, 491, 529, 558, 586, 602, 948
HapII CCGG 3 cut(s) 212, 218, 1202
HgaI GACGC 1 cut(s) 700
HhaI GCGC 1 cut(s) 223
Hin1I GRCGYC 1 cut(s) 221
Hin6I GCGC 1 cut(s) 221
HinP1I GCGC 1 cut(s) 221
HinfI GANTC 5 cut(s) 8, 454, 607, 847, 1217
HpaII CCGG 3 cut(s) 212, 218, 1202
HphI GGTGA 4 cut(s) 233, 671, 709, 1366
Hpy166II GTNNAC 1 cut(s) 427
Hpy188III TCNNGA 4 cut(s) 472, 611, 800, 1300
Hpy8I GTNNAC 1 cut(s) 427
Hpy99I CGWCG 1 cut(s) 229
HpyAV CCTTC 7 cut(s) 135, 494, 850, 878, 1042, 1306, 1362
HpyCH4III ACNGT 4 cut(s) 67, 293, 980, 985
HpyCH4IV ACGT 2 cut(s) 536, 823
HpyCH4V TGCA 9 cut(s) 196, 443, 867, 872, 1007, 1190, 1226, 1345, 1360
HpyF10VI GCNNNNNNNGC 5 cut(s) 998, 1007, 1187, 1378, 1444
HpyF3I CTNAG 5 cut(s) 10, 284, 1089, 1395, 1429
HpySE526I ACGT 2 cut(s) 536, 823
Hsp92I GRCGYC 1 cut(s) 221
HspAI GCGC 1 cut(s) 221
KasI GGCGCC 1 cut(s) 220
Kzo9I GATC 1 cut(s) 1110
LmnI GCTCC 2 cut(s) 773, 1026
Lsp1109I GCAGC 1 cut(s) 1019
LweI GCATC 2 cut(s) 818, 1347
MaeI CTAG 2 cut(s) 900, 1167
MaeII ACGT 2 cut(s) 536, 823
MaeIII GTNAC 3 cut(s) 40, 61, 287
MalI GATC 1 cut(s) 1112
MboI GATC 1 cut(s) 1110
MhlI GDGCHC 2 cut(s) 1142, 1383
MlsI TGGCCA 2 cut(s) 338, 602
MluCI AATT 4 cut(s) 379, 1209, 1272, 1469
MluNI TGGCCA 2 cut(s) 338, 602
Mly113I GGCGCC 1 cut(s) 221
MlyI GAGTC 2 cut(s) 2, 463
MmeI TCCRAC 1 cut(s) 844
Mox20I TGGCCA 2 cut(s) 338, 602
Mph1103I ATGCAT 1 cut(s) 445
MroXI GAANNNNTTC 1 cut(s) 1083
MscI TGGCCA 2 cut(s) 338, 602
MseI TTAA 5 cut(s) 86, 891, 929, 1248, 1440
MslI CAYNNNNRTG 1 cut(s) 246
Msp20I TGGCCA 2 cut(s) 338, 602
MspI CCGG 3 cut(s) 212, 218, 1202
MspR9I CCNGG 1 cut(s) 1202
Mva1269I GAATGC 1 cut(s) 1192
MvnI CGCG 1 cut(s) 711
MwoI GCNNNNNNNGC 5 cut(s) 998, 1007, 1187, 1378, 1444
NarI GGCGCC 1 cut(s) 221
NciI CCSGG 1 cut(s) 1202
NcoI CCATGG 1 cut(s) 1012
NdeI CATATG 1 cut(s) 1416
NdeII GATC 1 cut(s) 1110
NlaIV GGNNCC 2 cut(s) 216, 222
NmuCI GTSAC 1 cut(s) 287
NsiI ATGCAT 1 cut(s) 445
NspI RCATGY 1 cut(s) 200
PagI TCATGA 1 cut(s) 799
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 233
PctI GAATGC 1 cut(s) 1192
PdmI GAANNNNTTC 1 cut(s) 1083
PfeI GAWTC 3 cut(s) 607, 847, 1217
PflFI GACNNNGTC 1 cut(s) 705
PflMI CCANNNNNTGG 1 cut(s) 787
PkrI GCNGC 1 cut(s) 1009
PleI GAGTC 2 cut(s) 2, 462
PluTI GGCGCC 1 cut(s) 224
PpsI GAGTC 2 cut(s) 2, 462
Ppu21I YACGTR 1 cut(s) 537
Psp124BI GAGCTC 1 cut(s) 1383
PspN4I GGNNCC 2 cut(s) 216, 222
PspPI GGNCC 6 cut(s) 251, 489, 517, 527, 556, 947
PsyI GACNNNGTC 1 cut(s) 705
RsaI GTAC 8 cut(s) 33, 282, 510, 539, 738, 842, 982, 1334
RsaNI GTAC 8 cut(s) 32, 281, 509, 538, 737, 841, 981, 1333
RseI CAYNNNNRTG 1 cut(s) 246
SacI GAGCTC 1 cut(s) 1383
SaqAI TTAA 5 cut(s) 86, 891, 929, 1248, 1440
SatI GCNGC 1 cut(s) 1008
Sau3AI GATC 1 cut(s) 1110
Sau96I GGNCC 6 cut(s) 251, 489, 517, 527, 556, 947
ScaI AGTACT 2 cut(s) 33, 282
SchI GAGTC 2 cut(s) 2, 463
ScrFI CCNGG 1 cut(s) 1202
SduI GDGCHC 2 cut(s) 1142, 1383
SfaNI GCATC 2 cut(s) 818, 1347
SfcI CTRYAG 1 cut(s) 493
SfoI GGCGCC 1 cut(s) 222
SgrAI CRCCGGYG 1 cut(s) 217
SinI GGWCC 1 cut(s) 517
SmiMI CAYNNNNRTG 1 cut(s) 246
SmlI CTYRAG 1 cut(s) 276
SmoI CTYRAG 1 cut(s) 276
SnaBI TACGTA 1 cut(s) 537
Sse9I AATT 4 cut(s) 379, 1209, 1272, 1469
SsiI CCGC 2 cut(s) 233, 1277
SspDI GGCGCC 1 cut(s) 220
SspI AATATT 1 cut(s) 1308
SspMI CTAG 2 cut(s) 900, 1167
SstI GAGCTC 1 cut(s) 1383
StyD4I CCNGG 1 cut(s) 1200
StyI CCWWGG 1 cut(s) 1012
TaaI ACNGT 4 cut(s) 67, 293, 980, 985
TaiI ACGT 2 cut(s) 539, 826
TaqI TCGA 2 cut(s) 700, 1456
TasI AATT 4 cut(s) 379, 1209, 1272, 1469
TatI WGTACW 3 cut(s) 31, 280, 736
TfiI GAWTC 3 cut(s) 607, 847, 1217
Tru1I TTAA 5 cut(s) 86, 891, 929, 1248, 1440
Tru9I TTAA 5 cut(s) 86, 891, 929, 1248, 1440
TscAI CASTG 2 cut(s) 298, 1437
TseFI GTSAC 1 cut(s) 287
TseI GCWGC 1 cut(s) 1007
Tsp45I GTSAC 1 cut(s) 287
TspDTI ATGAA 9 cut(s) 437, 553, 586, 686, 719, 869, 1077, 1092, 1199
TspRI CASTG 2 cut(s) 298, 1437
Tth111I GACNNNGTC 1 cut(s) 705
Van91I CCANNNNNTGG 1 cut(s) 787
VpaK11BI GGWCC 1 cut(s) 517
XagI CCTNNNNNAGG 1 cut(s) 391
XapI RAATTY 2 cut(s) 1272, 1469
XceI RCATGY 1 cut(s) 200
XmnI GAANNNNTTC 1 cut(s) 1083
XspI CTAG 2 cut(s) 900, 1167
ZrmI AGTACT 2 cut(s) 33, 282
Zsp2I ATGCAT 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.