Rh3AG067500

protease Do-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0005022
Physical Location & Seq
Reverse (-)
4693715 .. 4696849
3135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG067500.1

Sequence Viewer

Length: 1257 bp
ATGAGTCATTTTCTGAGGAAGGTTTTGATTGCAAACCGGAATCGACATCGGATTCTGGCCATTGCTGCCGTTGGTTCGGCTTTTCTGTATGCCAAAGGGGACGAGAATTTCAATTCGTCATTCTTGGTGTCAATTCCTGCACCATGGCGTGAATCGTTATCACTGCCGAGGCAAAACTGGCCCTTTGGAGTTGTGCCATTATTTTCTGTTGCACATGGTTCGGGTCCTTCATCGGATGTATCTGGTTTCTCCGTAGCTCCAGAGAGCCCAAAGCCATGCTGCGGATGTTTGGGCAAAGATACAATTGCAAAGGCCGCTGCGAAGGTCGGTCCTGCGGTTGTCAATATATCTCTTCAACAGGGTATATACGGGGTTGGTGTTGGAAAGGGTATAGGCTCTGGAACTATAATTGATGAGGATGGCACGATCCTAACATGTGCTCATGCTGTGGTTGATTTTCATGGGCTGAGAGCTTCATCAAAGGGGAAGGTTGGTGTAACTTTGCAAGATGGTCGGACATTTGAGGGTACTGTGGTGAATGCAGATCTACAATCTGATGTTGCTATTGTGAAGATCAATTCTAAAACCCCCCTTCCAACAGCAAAGTTGGGCACCTCAAGCAAACTCCAACCTGGGGATTGGGTGATAGCAGTTGGCTGTCCACTTTCGCTTCAGAATACCGTCACAGCTGGCATTGTAAGTTGTGTCGACCGCAAAAGTAGTGATTTGGGTCTTGGTGGACTGCGGAGAGAGTACTTACAGACAGATTGCGCAATCAACCCGGGGAATTCTGGGGGACCTCTAGTAAATATGGATGGAGAAGTTATTGGTGTTAATATCATGAAAGTACTTGCTGCCGATGGATTGAGTTTCGCTGTACCGATTGACTCAATCGCCAAGATAATGGAGCACTTTAGAAAAAATGGGAGGGTTGTTCGGCCTTGGCTTGGACTGAAGATGATTGACCTAAATGATATGATAATTTCTCAGCTTAAAGACAGAGATGGTTCATTTCCAAATATAAAAAGAGGGATTCTTGTACCTATGGTAACTCCTGGGTCACCTGCTGAGCGTGCTGGGTTTCGTCCCGGTGATGTTGTTGTCGAATTTGATGGAAAGCCTGTTGGAAGCATCACAGAGATCACTGAAATTATGGGAGATAGAGTTGGAGTACCATTGAAGGTGGTTGTGAAGAGGGCGAAAGACGAGTTGGTGACTTTGACTGTAATCGCAGAGGAGTCCCATACAGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04210 ko04214 ko04215 ko05012 map04210 map04214 map04215 map05012
Pfam Domains
Protein Families
PDZ_2 Trypsin_2

Protein Analysis

418

Amino Acids

44.11

Weight (kDa)

8.6

Isoelectric Point (pI)

34.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trypsin PF00089 109 - 300 2.6e-18 Trypsin
Trypsin_2 PF13365 132 - 278 1.1e-33 Trypsin-like peptidase domain
PDZ_2 PF13180 325 - 409 4.8e-11 PDZ domain
PDZ_6 PF17820 350 - 397 1.5e-11 PDZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1072
Acc16I TGCGCA 1 cut(s) 772
Acc36I ACCTGC 1 cut(s) 1072
AccB1I GGYRCC 1 cut(s) 611
AccI GTMKAC 1 cut(s) 708
AciI CCGC 5 cut(s) 282, 315, 335, 712, 745
AclWI GGATC 1 cut(s) 421
AcoI YGGCCR 1 cut(s) 57
AcsI RAATTY 3 cut(s) 106, 787, 1106
AcuI CTGAAG 2 cut(s) 656, 974
AfaI GTAC 6 cut(s) 529, 755, 849, 879, 1041, 1173
AfiI CCNNNNNNNGG 3 cut(s) 281, 634, 947
AflIII ACRYGT 1 cut(s) 434
AgsI TTSAA 3 cut(s) 112, 356, 1180
AjnI CCWGG 2 cut(s) 631, 1054
AjuI GAANNNNNNNTTGG 2 cut(s) 1193, 1225
AluBI AGCT 4 cut(s) 257, 473, 689, 991
AluI AGCT 4 cut(s) 257, 473, 689, 991
Alw21I GWGCWC 2 cut(s) 442, 912
AlwI GGATC 1 cut(s) 421
Ama87I CYCGRG 1 cut(s) 781
AoxI GGCC 4 cut(s) 57, 179, 312, 938
ApeKI GCWGC 4 cut(s) 65, 279, 317, 854
ApoI RAATTY 3 cut(s) 106, 787, 1106
AspLEI GCGC 1 cut(s) 773
AspS9I GGNCC 4 cut(s) 180, 224, 329, 797
AsuC2I CCSGG 3 cut(s) 782, 783, 1089
AsuHPI GGTGA 5 cut(s) 547, 655, 1053, 1103, 1225
AvaI CYCGRG 1 cut(s) 781
AvaII GGWCC 3 cut(s) 224, 329, 797
BaeGI GKGCMC 1 cut(s) 614
BaeI ACNNNNGTAYC 2 cut(s) 519, 552
BalI TGGCCA 1 cut(s) 59
BanI GGYRCC 1 cut(s) 611
BanII GRGCYC 1 cut(s) 269
Bbv12I GWGCWC 2 cut(s) 442, 912
BbvI GCAGC 4 cut(s) 52, 266, 304, 841
BccI CCATC 6 cut(s) 413, 503, 809, 854, 998, 1106
BceAI ACGGC 1 cut(s) 53
BcgI CGANNNNNNTGC 4 cut(s) 201, 235, 494, 528
BciT130I CCWGG 2 cut(s) 633, 1056
BcnI CCSGG 3 cut(s) 782, 783, 1089
BfaI CTAG 1 cut(s) 803
BfuAI ACCTGC 1 cut(s) 1072
BglII AGATCT 1 cut(s) 544
BisI GCNGC 5 cut(s) 66, 280, 315, 318, 855
BlpI GCTNAGC 1 cut(s) 1068
BlsI GCNGC 5 cut(s) 67, 281, 316, 319, 856
BmcAI AGTACT 2 cut(s) 755, 849
Bme1390I CCNGG 5 cut(s) 633, 782, 783, 1056, 1089
Bme18I GGWCC 3 cut(s) 224, 329, 797
BmeT110I CYCGRG 1 cut(s) 781
BmgT120I GGNCC 4 cut(s) 180, 224, 329, 797
BmiI GGNNCC 3 cut(s) 225, 613, 798
BmrFI CCNGG 5 cut(s) 633, 782, 783, 1056, 1089
BmsI GCATC 1 cut(s) 1140
BpmI CTGGAG 1 cut(s) 243
Bpu1102I GCTNAGC 1 cut(s) 1068
BpuEI CTTGAG 1 cut(s) 601
BpuMI CCSGG 3 cut(s) 782, 783, 1089
BsaJI CCNNGG 7 cut(s) 143, 167, 632, 781, 782, 941, 1055
BsaWI WCCGGW 1 cut(s) 36
BsaXI ACNNNNNCTCC 2 cut(s) 1149, 1179
Bsc4I CCNNNNNNNGG 3 cut(s) 281, 634, 947
Bse1I ACTGG 1 cut(s) 182
Bse3DI GCAATG 1 cut(s) 60
BseBI CCWGG 2 cut(s) 633, 1056
BseDI CCNNGG 7 cut(s) 143, 167, 632, 781, 782, 941, 1055
BseGI GGATG 4 cut(s) 241, 290, 424, 820
BseLI CCNNNNNNNGG 3 cut(s) 281, 634, 947
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 4 cut(s) 5, 458, 1001, 1059
BseNI ACTGG 1 cut(s) 182
BseRI GAGGAG 1 cut(s) 1250
BseSI GKGCMC 1 cut(s) 614
BseXI GCAGC 4 cut(s) 52, 266, 304, 841
BseYI CCCAGC 1 cut(s) 1076
BsgI GTGCAG 1 cut(s) 123
Bsh1285I CGRYCG 1 cut(s) 712
BshFI GGCC 4 cut(s) 59, 181, 314, 940
BshNI GGYRCC 1 cut(s) 611
BsiEI CGRYCG 1 cut(s) 712
BsiHKAI GWGCWC 2 cut(s) 442, 912
BsiHKCI CYCGRG 1 cut(s) 781
BsiSI CCGG 3 cut(s) 37, 782, 1089
BslFI GGGAC 4 cut(s) 113, 810, 1071, 1225
BslI CCNNNNNNNGG 3 cut(s) 281, 634, 947
BsmFI GGGAC 4 cut(s) 113, 810, 1071, 1225
BsmI GAATGC 1 cut(s) 544
BsnI GGCC 4 cut(s) 59, 181, 314, 940
BsoBI CYCGRG 1 cut(s) 781
Bsp1286I GDGCHC 4 cut(s) 269, 442, 614, 912
Bsp143I GATC 4 cut(s) 426, 544, 573, 1140
Bsp1720I GCTNAGC 1 cut(s) 1068
Bsp19I CCATGG 1 cut(s) 143
BspACI CCGC 5 cut(s) 282, 315, 335, 712, 745
BspANI GGCC 4 cut(s) 59, 181, 314, 940
BspCNI CTCAG 4 cut(s) 6, 459, 1000, 1060
BspHI TCATGA 1 cut(s) 840
BspLI GGNNCC 3 cut(s) 225, 613, 798
BspMI ACCTGC 1 cut(s) 1072
BspPI GGATC 1 cut(s) 421
BspT107I GGYRCC 1 cut(s) 611
BsrDI GCAATG 1 cut(s) 60
BsrI ACTGG 1 cut(s) 182
BssECI CCNNGG 7 cut(s) 143, 167, 632, 781, 782, 941, 1055
BssMI GATC 4 cut(s) 426, 544, 573, 1140
BssT1I CCWWGG 2 cut(s) 143, 941
Bst2UI CCWGG 2 cut(s) 633, 1056
Bst4CI ACNGT 3 cut(s) 532, 682, 1225
Bst6I CTCTTC 2 cut(s) 357, 1187
BstC8I GCNNGC 2 cut(s) 691, 1074
BstDEI CTNAG 4 cut(s) 14, 467, 987, 1068
BstDSI CCRYGG 1 cut(s) 143
BstEII GGTNACC 1 cut(s) 1059
BstF5I GGATG 4 cut(s) 241, 290, 424, 820
BstHHI GCGC 1 cut(s) 773
BstKTI GATC 4 cut(s) 429, 547, 576, 1143
BstMBI GATC 4 cut(s) 426, 544, 573, 1140
BstMCI CGRYCG 1 cut(s) 712
BstMWI GCNNNNNNNGC 5 cut(s) 65, 178, 314, 618, 1073
BstNI CCWGG 2 cut(s) 633, 1056
BstNSI RCATGY 1 cut(s) 438
BstPI GGTNACC 1 cut(s) 1059
BstSCI CCNGG 5 cut(s) 631, 780, 781, 1054, 1087
BstSLI GKGCMC 1 cut(s) 614
BstV1I GCAGC 4 cut(s) 52, 266, 304, 841
BstX2I RGATCY 1 cut(s) 544
BstXI CCANNNNNNTGG 1 cut(s) 904
BstYI RGATCY 1 cut(s) 544
BsuRI GGCC 4 cut(s) 59, 181, 314, 940
BtgI CCRYGG 1 cut(s) 143
BtsCI GGATG 4 cut(s) 241, 290, 424, 820
BtsI GCAGTG 1 cut(s) 161
BtsIMutI CAGTG 2 cut(s) 161, 1143
BveI ACCTGC 1 cut(s) 1072
Cac8I GCNNGC 2 cut(s) 691, 1074
CciI TCATGA 1 cut(s) 840
CfoI GCGC 1 cut(s) 773
Cfr13I GGNCC 4 cut(s) 180, 224, 329, 797
Cfr9I CCCGGG 1 cut(s) 781
Csp6I GTAC 6 cut(s) 528, 754, 848, 878, 1040, 1172
CviAII CATG 7 cut(s) 144, 215, 276, 435, 443, 461, 841
CviQI GTAC 6 cut(s) 528, 754, 848, 878, 1040, 1172
DdeI CTNAG 4 cut(s) 14, 467, 987, 1068
DpnI GATC 4 cut(s) 428, 546, 575, 1142
DpnII GATC 4 cut(s) 426, 544, 573, 1140
EaeI YGGCCR 1 cut(s) 57
Eam1104I CTCTTC 2 cut(s) 357, 1187
EarI CTCTTC 2 cut(s) 357, 1187
Eco130I CCWWGG 2 cut(s) 143, 941
Eco24I GRGCYC 1 cut(s) 269
Eco47I GGWCC 3 cut(s) 224, 329, 797
Eco57I CTGAAG 2 cut(s) 656, 974
Eco88I CYCGRG 1 cut(s) 781
Eco91I GGTNACC 1 cut(s) 1059
EcoO109I RGGNCCY 2 cut(s) 224, 797
EcoO65I GGTNACC 1 cut(s) 1059
EcoRI GAATTC 1 cut(s) 787
EcoRII CCWGG 2 cut(s) 631, 1054
EcoT14I CCWWGG 2 cut(s) 143, 941
EcoT38I GRGCYC 1 cut(s) 269
ErhI CCWWGG 2 cut(s) 143, 941
FaeI CATG 7 cut(s) 147, 218, 279, 438, 446, 464, 844
FaqI GGGAC 4 cut(s) 113, 810, 1071, 1225
FatI CATG 7 cut(s) 143, 214, 275, 434, 442, 460, 840
FblI GTMKAC 1 cut(s) 708
Fnu4HI GCNGC 5 cut(s) 66, 280, 315, 318, 855
FokI GGATG 4 cut(s) 248, 297, 431, 827
FriOI GRGCYC 1 cut(s) 269
Fsp4HI GCNGC 5 cut(s) 66, 280, 315, 318, 855
FspBI CTAG 1 cut(s) 803
FspI TGCGCA 1 cut(s) 772
GlaI GCGC 1 cut(s) 772
GluI GCNGC 5 cut(s) 66, 280, 315, 318, 855
GsaI CCCAGC 1 cut(s) 1080
GsuI CTGGAG 1 cut(s) 243
HaeIII GGCC 4 cut(s) 59, 181, 314, 940
HapII CCGG 3 cut(s) 37, 782, 1089
HhaI GCGC 1 cut(s) 773
Hin1II CATG 7 cut(s) 147, 218, 279, 438, 446, 464, 844
Hin6I GCGC 1 cut(s) 771
HinP1I GCGC 1 cut(s) 771
HincII GTYRAC 1 cut(s) 709
HindII GTYRAC 1 cut(s) 709
HinfI GANTC 7 cut(s) 4, 40, 52, 152, 887, 1033, 1238
HpaII CCGG 3 cut(s) 37, 782, 1089
HphI GGTGA 5 cut(s) 547, 655, 1053, 1103, 1225
Hpy166II GTNNAC 3 cut(s) 662, 709, 740
Hpy188I TCNGA 6 cut(s) 15, 51, 235, 516, 556, 675
Hpy188III TCNNGA 3 cut(s) 260, 399, 841
Hpy8I GTNNAC 3 cut(s) 662, 709, 740
HpyAV CCTTC 6 cut(s) 13, 237, 316, 481, 602, 1174
HpyCH4III ACNGT 3 cut(s) 532, 682, 1225
HpyCH4V TGCA 6 cut(s) 32, 140, 212, 308, 505, 542
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 178, 314, 618, 1073
HpyF3I CTNAG 4 cut(s) 14, 467, 987, 1068
Hsp92II CATG 7 cut(s) 147, 218, 279, 438, 446, 464, 844
HspAI GCGC 1 cut(s) 771
Kzo9I GATC 4 cut(s) 426, 544, 573, 1140
LmnI GCTCC 2 cut(s) 262, 907
Lsp1109I GCAGC 4 cut(s) 52, 266, 304, 841
LweI GCATC 1 cut(s) 1140
MaeI CTAG 1 cut(s) 803
MaeIII GTNAC 5 cut(s) 496, 682, 1048, 1059, 1213
MalI GATC 4 cut(s) 428, 546, 575, 1142
MboI GATC 4 cut(s) 426, 544, 573, 1140
MboII GAAGA 4 cut(s) 344, 583, 967, 1204
MfeI CAATTG 1 cut(s) 303
MflI RGATCY 1 cut(s) 544
MhlI GDGCHC 4 cut(s) 269, 442, 614, 912
MlsI TGGCCA 1 cut(s) 59
MluNI TGGCCA 1 cut(s) 59
MlyI GAGTC 3 cut(s) 13, 881, 1247
MmeI TCCRAC 6 cut(s) 361, 494, 620, 652, 1105, 1147
Mox20I TGGCCA 1 cut(s) 59
MscI TGGCCA 1 cut(s) 59
MseI TTAA 2 cut(s) 834, 993
Msp20I TGGCCA 1 cut(s) 59
MspA1I CMGCKG 2 cut(s) 317, 689
MspI CCGG 3 cut(s) 37, 782, 1089
MspR9I CCNGG 5 cut(s) 633, 782, 783, 1056, 1089
MunI CAATTG 1 cut(s) 303
Mva1269I GAATGC 1 cut(s) 544
MvaI CCWGG 2 cut(s) 633, 1056
MwoI GCNNNNNNNGC 5 cut(s) 65, 178, 314, 618, 1073
NciI CCSGG 3 cut(s) 782, 783, 1089
NcoI CCATGG 1 cut(s) 143
NdeII GATC 4 cut(s) 426, 544, 573, 1140
NlaIII CATG 7 cut(s) 147, 218, 279, 438, 446, 464, 844
NlaIV GGNNCC 3 cut(s) 225, 613, 798
NmeAIII GCCGAG 1 cut(s) 192
NmuCI GTSAC 3 cut(s) 682, 1059, 1213
NsbI TGCGCA 1 cut(s) 772
NspI RCATGY 1 cut(s) 438
PagI TCATGA 1 cut(s) 840
PaqCI CACCTGC 1 cut(s) 1072
PciI ACATGT 1 cut(s) 434
PctI GAATGC 1 cut(s) 544
PfeI GAWTC 4 cut(s) 40, 52, 152, 1033
PkrI GCNGC 5 cut(s) 67, 281, 316, 319, 856
PleI GAGTC 3 cut(s) 12, 881, 1246
PpsI GAGTC 3 cut(s) 12, 881, 1246
PpuMI RGGWCCY 2 cut(s) 224, 797
PscI ACATGT 1 cut(s) 434
Psp5II RGGWCCY 2 cut(s) 224, 797
Psp6I CCWGG 2 cut(s) 631, 1054
PspEI GGTNACC 1 cut(s) 1059
PspFI CCCAGC 1 cut(s) 1076
PspGI CCWGG 2 cut(s) 631, 1054
PspN4I GGNNCC 3 cut(s) 225, 613, 798
PspPI GGNCC 4 cut(s) 180, 224, 329, 797
PspPPI RGGWCCY 2 cut(s) 224, 797
PsuI RGATCY 1 cut(s) 544
PvuII CAGCTG 1 cut(s) 689
RsaI GTAC 6 cut(s) 529, 755, 849, 879, 1041, 1173
RsaNI GTAC 6 cut(s) 528, 754, 848, 878, 1040, 1172
SalI GTCGAC 1 cut(s) 707
SaqAI TTAA 2 cut(s) 834, 993
SatI GCNGC 5 cut(s) 66, 280, 315, 318, 855
Sau3AI GATC 4 cut(s) 426, 544, 573, 1140
Sau96I GGNCC 4 cut(s) 180, 224, 329, 797
ScaI AGTACT 2 cut(s) 755, 849
SchI GAGTC 3 cut(s) 13, 881, 1247
ScrFI CCNGG 5 cut(s) 633, 782, 783, 1056, 1089
SduI GDGCHC 4 cut(s) 269, 442, 614, 912
SfaNI GCATC 1 cut(s) 1140
SinI GGWCC 3 cut(s) 224, 329, 797
SmaI CCCGGG 1 cut(s) 783
SmlI CTYRAG 1 cut(s) 616
SmoI CTYRAG 1 cut(s) 616
SsiI CCGC 5 cut(s) 282, 315, 335, 712, 745
SspMI CTAG 1 cut(s) 803
StyD4I CCNGG 5 cut(s) 631, 780, 781, 1054, 1087
StyI CCWWGG 2 cut(s) 143, 941
TaaI ACNGT 3 cut(s) 532, 682, 1225
TaqI TCGA 3 cut(s) 43, 708, 1104
TaqII GACCGA 1 cut(s) 317
TatI WGTACW 2 cut(s) 753, 847
TauI GCSGC 1 cut(s) 317
TfiI GAWTC 4 cut(s) 40, 52, 152, 1033
Tru1I TTAA 2 cut(s) 834, 993
Tru9I TTAA 2 cut(s) 834, 993
TscAI CASTG 2 cut(s) 168, 1150
TseFI GTSAC 3 cut(s) 682, 1059, 1213
TseI GCWGC 4 cut(s) 65, 279, 317, 854
Tsp45I GTSAC 3 cut(s) 682, 1059, 1213
TspDTI ATGAA 5 cut(s) 219, 449, 465, 857, 999
TspGWI ACGGA 1 cut(s) 241
TspMI CCCGGG 1 cut(s) 781
TspRI CASTG 2 cut(s) 168, 1150
VpaK11BI GGWCC 3 cut(s) 224, 329, 797
XapI RAATTY 3 cut(s) 106, 787, 1106
XceI RCATGY 1 cut(s) 438
XmaI CCCGGG 1 cut(s) 781
XmiI GTMKAC 1 cut(s) 708
XspI CTAG 1 cut(s) 803
ZrmI AGTACT 2 cut(s) 755, 849
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.