RLG00000025355

protease Do-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0005022
Physical Location & Seq
Forward (+)
45244135 .. 45247231
3097 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025355

Sequence Viewer

Length: 1269 bp
ATGAGTCATTTTCTGAAGAAGGTTTTGATTGCAAACCGGAACCGACATGGGATTCTGGCCATTGCTGCCGTTGGTTCGGCTTATTTGTATGCTAAAGGGGACGAGAATTTCAATTCGTCATTCTTGGTGTCAATTCCTGCACCATGGCGTGAATCGTTGTCACTGCCGAGGCAAAACTGGCCATTTGGAGTTGTACCATTATTTTCTGTTGCGCATGGTTCGGGTCCTTCATCGGATGTAGGGAAGGATGTATCTGGTTTCTCCGTAGCTGCAGAGAGCCCAAAGCCATGCTATGGATGTTTGGGCAAAGATACAATTGCAAAGGCCGCTGCGAAGGTCGGTCCTGCTGTTGTCAATATATCTCTTCAACAGGGTATATATGGGGTTGGTGTTGGAAAGGGTATAGGCTCTGGAACTATAATCGATGAGGATGGCACGATCCTAACATGTGCTCATGCTGTGGTTGATTGTCACGGGCTGAGAGCTTCATCAAAGGGGAAGGTTGGTGTAACTTTGCAAGATGGTCGGACATTTGAGGGTACTGTGGTGAATGCAGATCTGCAATCTGATGTTGCTATTGTGAAGATCAATTCTAAAACCCCCCTTCCAACAGCAAAGTTGGGCACCTCAAGTCAACTCCAACCTGGGGATTGGGTGATAGCAGTTGGCTGTCCACTTTCGCTTCAGAATACCGTCACAGCTGGCATTGTAAGTTGTGTCGACCGCAAAAGTAGTGATTTGGGTCTTGGTGGACTGCGGAGAGAGTACTTACAGACAGATTGCGCAATCAACCCGGGGAATTCTGGGGGACCTCTAGTAAATATGGATGGAGAAGTTATTGGTGTTAATATCATGAAAGTACTTGCTGCCGATGGATTGAGTTTTGCTGTACCGATTGACTCAATCGCCAAGATAATGGAGCACTTTAGAAAAAATGGGAGGGTTGTTCGGCCTTGGCTTGGACTGAAGATGATTGACCTAAATGATATGATAATTTCTCAGCTTAAAGAAAGAGATAGTTCATTTCCAAATATAAAAATAGGGATTCTCGTACCTATGGTAACTCCTGGGTCACCTGCCGAGCGTGCTGGGTTTCGTCCCGGTGATGTTGTTGTCGAATTTGATGGAAAGCCTGTTGCAAGCATCACAGAGATCACTGAAATTATGGGAGATAGAGTTGGAGTACCATTGAAGGTGGTTGTGAAGAGGGCGAAAGATGAGTTGGTGACTTTGACTGTAATCGCAGAGGAGTCCCATACAGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04210 ko04214 ko04215 ko05012 map04210 map04214 map04215 map05012
Pfam Domains
Protein Families
PDZ_2 Trypsin_2

Protein Analysis

423

Amino Acids

44.4

Weight (kDa)

7.6

Isoelectric Point (pI)

33.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trypsin PF00089 113 - 304 4.7e-18 Trypsin
Trypsin_2 PF13365 136 - 282 2.9e-34 Trypsin-like peptidase domain
PDZ_2 PF13180 320 - 413 6.2e-11 PDZ domain
PDZ_6 PF17820 354 - 401 4.4e-11 PDZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1084
Acc16I TGCGCA 2 cut(s) 213, 784
Acc36I ACCTGC 1 cut(s) 1084
AccB1I GGYRCC 1 cut(s) 623
AccB7I CCANNNNNTGG 1 cut(s) 293
AccI GTMKAC 1 cut(s) 720
AciI CCGC 3 cut(s) 327, 724, 757
AclWI GGATC 1 cut(s) 433
AcoI YGGCCR 2 cut(s) 57, 179
AcsI RAATTY 3 cut(s) 106, 799, 1118
AcuI CTGAAG 3 cut(s) 35, 668, 986
AfaI GTAC 7 cut(s) 195, 541, 767, 861, 891, 1053, 1185
AfiI CCNNNNNNNGG 3 cut(s) 293, 646, 959
AflIII ACRYGT 1 cut(s) 446
AgsI TTSAA 3 cut(s) 112, 368, 1192
AjnI CCWGG 2 cut(s) 643, 1066
AjuI GAANNNNNNNTTGG 2 cut(s) 1205, 1237
AluBI AGCT 4 cut(s) 269, 485, 701, 1003
AluI AGCT 4 cut(s) 269, 485, 701, 1003
Alw21I GWGCWC 2 cut(s) 454, 924
AlwI GGATC 1 cut(s) 433
Ama87I CYCGRG 1 cut(s) 793
AoxI GGCC 4 cut(s) 57, 179, 324, 950
ApeKI GCWGC 4 cut(s) 65, 269, 329, 866
ApoI RAATTY 3 cut(s) 106, 799, 1118
AspLEI GCGC 2 cut(s) 214, 785
AspS9I GGNCC 3 cut(s) 224, 341, 809
AsuC2I CCSGG 3 cut(s) 794, 795, 1101
AsuHPI GGTGA 5 cut(s) 559, 667, 1065, 1115, 1237
AvaI CYCGRG 1 cut(s) 793
AvaII GGWCC 3 cut(s) 224, 341, 809
BaeGI GKGCMC 1 cut(s) 626
BaeI ACNNNNGTAYC 2 cut(s) 531, 564
BalI TGGCCA 2 cut(s) 59, 181
BanI GGYRCC 1 cut(s) 623
BanII GRGCYC 1 cut(s) 281
Bbv12I GWGCWC 2 cut(s) 454, 924
BbvI GCAGC 4 cut(s) 52, 256, 316, 853
BccI CCATC 5 cut(s) 425, 515, 821, 866, 1118
BceAI ACGGC 1 cut(s) 53
BcgI CGANNNNNNTGC 2 cut(s) 506, 540
BciT130I CCWGG 2 cut(s) 645, 1068
BcnI CCSGG 3 cut(s) 794, 795, 1101
BfaI CTAG 1 cut(s) 815
BfmI CTRYAG 1 cut(s) 270
BfuAI ACCTGC 1 cut(s) 1084
BglII AGATCT 1 cut(s) 556
BisI GCNGC 5 cut(s) 66, 270, 327, 330, 867
BlsI GCNGC 5 cut(s) 67, 271, 328, 331, 868
BmcAI AGTACT 2 cut(s) 767, 861
Bme1390I CCNGG 5 cut(s) 645, 794, 795, 1068, 1101
Bme18I GGWCC 3 cut(s) 224, 341, 809
BmeT110I CYCGRG 1 cut(s) 793
BmgT120I GGNCC 3 cut(s) 224, 341, 809
BmiI GGNNCC 4 cut(s) 41, 225, 625, 810
BmrFI CCNGG 5 cut(s) 645, 794, 795, 1068, 1101
BmsI GCATC 1 cut(s) 1152
BpuEI CTTGAG 1 cut(s) 613
BpuMI CCSGG 3 cut(s) 794, 795, 1101
Bsa29I ATCGAT 1 cut(s) 423
BsaJI CCNNGG 7 cut(s) 143, 167, 644, 793, 794, 953, 1067
BsaWI WCCGGW 1 cut(s) 36
BsaXI ACNNNNNCTCC 2 cut(s) 1161, 1191
Bsc4I CCNNNNNNNGG 3 cut(s) 293, 646, 959
Bse1I ACTGG 1 cut(s) 182
Bse3DI GCAATG 1 cut(s) 60
BseBI CCWGG 2 cut(s) 645, 1068
BseCI ATCGAT 1 cut(s) 423
BseDI CCNNGG 7 cut(s) 143, 167, 644, 793, 794, 953, 1067
BseGI GGATG 5 cut(s) 241, 253, 302, 436, 832
BseLI CCNNNNNNNGG 3 cut(s) 293, 646, 959
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 2 cut(s) 470, 1013
BseNI ACTGG 1 cut(s) 182
BseRI GAGGAG 1 cut(s) 1262
BseSI GKGCMC 1 cut(s) 626
BseXI GCAGC 4 cut(s) 52, 256, 316, 853
BseYI CCCAGC 1 cut(s) 1088
BsgI GTGCAG 1 cut(s) 123
Bsh1285I CGRYCG 1 cut(s) 724
BshFI GGCC 4 cut(s) 59, 181, 326, 952
BshNI GGYRCC 1 cut(s) 623
BshVI ATCGAT 1 cut(s) 423
BsiEI CGRYCG 1 cut(s) 724
BsiHKAI GWGCWC 2 cut(s) 454, 924
BsiHKCI CYCGRG 1 cut(s) 793
BsiSI CCGG 3 cut(s) 37, 794, 1101
BslFI GGGAC 4 cut(s) 113, 822, 1083, 1237
BslI CCNNNNNNNGG 3 cut(s) 293, 646, 959
BsmFI GGGAC 4 cut(s) 113, 822, 1083, 1237
BsmI GAATGC 1 cut(s) 556
BsnI GGCC 4 cut(s) 59, 181, 326, 952
BsoBI CYCGRG 1 cut(s) 793
Bsp1286I GDGCHC 4 cut(s) 281, 454, 626, 924
Bsp143I GATC 4 cut(s) 438, 556, 585, 1152
Bsp19I CCATGG 1 cut(s) 143
BspACI CCGC 3 cut(s) 327, 724, 757
BspANI GGCC 4 cut(s) 59, 181, 326, 952
BspCNI CTCAG 2 cut(s) 471, 1012
BspDI ATCGAT 1 cut(s) 423
BspHI TCATGA 1 cut(s) 852
BspLI GGNNCC 4 cut(s) 41, 225, 625, 810
BspMAI CTGCAG 1 cut(s) 274
BspMI ACCTGC 1 cut(s) 1084
BspPI GGATC 1 cut(s) 433
BspT107I GGYRCC 1 cut(s) 623
BsrDI GCAATG 1 cut(s) 60
BsrI ACTGG 1 cut(s) 182
BssECI CCNNGG 7 cut(s) 143, 167, 644, 793, 794, 953, 1067
BssMI GATC 4 cut(s) 438, 556, 585, 1152
BssT1I CCWWGG 2 cut(s) 143, 953
Bst2UI CCWGG 2 cut(s) 645, 1068
Bst4CI ACNGT 3 cut(s) 544, 694, 1237
Bst6I CTCTTC 2 cut(s) 369, 1199
BstC8I GCNNGC 3 cut(s) 703, 1086, 1141
BstDEI CTNAG 2 cut(s) 479, 999
BstDSI CCRYGG 1 cut(s) 143
BstEII GGTNACC 1 cut(s) 1071
BstF5I GGATG 5 cut(s) 241, 253, 302, 436, 832
BstHHI GCGC 2 cut(s) 214, 785
BstKTI GATC 4 cut(s) 441, 559, 588, 1155
BstMBI GATC 4 cut(s) 438, 556, 585, 1152
BstMCI CGRYCG 1 cut(s) 724
BstMWI GCNNNNNNNGC 4 cut(s) 65, 178, 326, 1085
BstNI CCWGG 2 cut(s) 645, 1068
BstNSI RCATGY 1 cut(s) 450
BstPI GGTNACC 1 cut(s) 1071
BstSCI CCNGG 5 cut(s) 643, 792, 793, 1066, 1099
BstSFI CTRYAG 1 cut(s) 270
BstSLI GKGCMC 1 cut(s) 626
BstV1I GCAGC 4 cut(s) 52, 256, 316, 853
BstX2I RGATCY 1 cut(s) 556
BstXI CCANNNNNNTGG 1 cut(s) 916
BstYI RGATCY 1 cut(s) 556
Bsu15I ATCGAT 1 cut(s) 423
BsuRI GGCC 4 cut(s) 59, 181, 326, 952
BsuTUI ATCGAT 1 cut(s) 423
BtgI CCRYGG 1 cut(s) 143
BtsCI GGATG 5 cut(s) 241, 253, 302, 436, 832
BtsI GCAGTG 1 cut(s) 161
BtsIMutI CAGTG 2 cut(s) 161, 1155
BveI ACCTGC 1 cut(s) 1084
Cac8I GCNNGC 3 cut(s) 703, 1086, 1141
CciI TCATGA 1 cut(s) 852
CfoI GCGC 2 cut(s) 214, 785
Cfr13I GGNCC 3 cut(s) 224, 341, 809
Cfr9I CCCGGG 1 cut(s) 793
ClaI ATCGAT 1 cut(s) 423
Csp6I GTAC 7 cut(s) 194, 540, 766, 860, 890, 1052, 1184
CviAII CATG 7 cut(s) 47, 144, 215, 288, 447, 455, 853
CviQI GTAC 7 cut(s) 194, 540, 766, 860, 890, 1052, 1184
DdeI CTNAG 2 cut(s) 479, 999
DpnI GATC 4 cut(s) 440, 558, 587, 1154
DpnII GATC 4 cut(s) 438, 556, 585, 1152
EaeI YGGCCR 2 cut(s) 57, 179
Eam1104I CTCTTC 2 cut(s) 369, 1199
EarI CTCTTC 2 cut(s) 369, 1199
Eco130I CCWWGG 2 cut(s) 143, 953
Eco24I GRGCYC 1 cut(s) 281
Eco47I GGWCC 3 cut(s) 224, 341, 809
Eco57I CTGAAG 3 cut(s) 35, 668, 986
Eco88I CYCGRG 1 cut(s) 793
Eco91I GGTNACC 1 cut(s) 1071
EcoO109I RGGNCCY 2 cut(s) 224, 809
EcoO65I GGTNACC 1 cut(s) 1071
EcoRI GAATTC 1 cut(s) 799
EcoRII CCWGG 2 cut(s) 643, 1066
EcoT14I CCWWGG 2 cut(s) 143, 953
EcoT38I GRGCYC 1 cut(s) 281
ErhI CCWWGG 2 cut(s) 143, 953
FaeI CATG 7 cut(s) 50, 147, 218, 291, 450, 458, 856
FaqI GGGAC 4 cut(s) 113, 822, 1083, 1237
FatI CATG 7 cut(s) 46, 143, 214, 287, 446, 454, 852
FblI GTMKAC 1 cut(s) 720
Fnu4HI GCNGC 5 cut(s) 66, 270, 327, 330, 867
FokI GGATG 5 cut(s) 248, 260, 309, 443, 839
FriOI GRGCYC 1 cut(s) 281
Fsp4HI GCNGC 5 cut(s) 66, 270, 327, 330, 867
FspBI CTAG 1 cut(s) 815
FspI TGCGCA 2 cut(s) 213, 784
GlaI GCGC 2 cut(s) 213, 784
GluI GCNGC 5 cut(s) 66, 270, 327, 330, 867
GsaI CCCAGC 1 cut(s) 1092
HaeIII GGCC 4 cut(s) 59, 181, 326, 952
HapII CCGG 3 cut(s) 37, 794, 1101
HhaI GCGC 2 cut(s) 214, 785
Hin1II CATG 7 cut(s) 50, 147, 218, 291, 450, 458, 856
Hin6I GCGC 2 cut(s) 212, 783
HinP1I GCGC 2 cut(s) 212, 783
HincII GTYRAC 2 cut(s) 635, 721
HindII GTYRAC 2 cut(s) 635, 721
HinfI GANTC 6 cut(s) 4, 52, 152, 899, 1045, 1250
HpaII CCGG 3 cut(s) 37, 794, 1101
HphI GGTGA 5 cut(s) 559, 667, 1065, 1115, 1237
Hpy166II GTNNAC 4 cut(s) 635, 674, 721, 752
Hpy188I TCNGA 5 cut(s) 15, 235, 528, 568, 687
Hpy188III TCNNGA 2 cut(s) 411, 853
Hpy8I GTNNAC 4 cut(s) 635, 674, 721, 752
HpyAV CCTTC 7 cut(s) 13, 237, 238, 328, 493, 614, 1186
HpyCH4III ACNGT 3 cut(s) 544, 694, 1237
HpyCH4V TGCA 8 cut(s) 32, 140, 272, 320, 517, 554, 562, 1139
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 178, 326, 1085
HpyF3I CTNAG 2 cut(s) 479, 999
Hsp92II CATG 7 cut(s) 50, 147, 218, 291, 450, 458, 856
HspAI GCGC 2 cut(s) 212, 783
Kzo9I GATC 4 cut(s) 438, 556, 585, 1152
LmnI GCTCC 1 cut(s) 919
Lsp1109I GCAGC 4 cut(s) 52, 256, 316, 853
LweI GCATC 1 cut(s) 1152
MaeI CTAG 1 cut(s) 815
MaeIII GTNAC 7 cut(s) 159, 470, 508, 694, 1060, 1071, 1225
MalI GATC 4 cut(s) 440, 558, 587, 1154
MboI GATC 4 cut(s) 438, 556, 585, 1152
MboII GAAGA 5 cut(s) 28, 356, 595, 979, 1216
MfeI CAATTG 1 cut(s) 315
MflI RGATCY 1 cut(s) 556
MhlI GDGCHC 4 cut(s) 281, 454, 626, 924
MlsI TGGCCA 2 cut(s) 59, 181
MluCI AATT 9 cut(s) 106, 112, 132, 315, 589, 799, 993, 1118, 1161
MluNI TGGCCA 2 cut(s) 59, 181
MlyI GAGTC 3 cut(s) 13, 893, 1259
MmeI TCCRAC 5 cut(s) 373, 506, 632, 664, 1159
MnlI CCTC 8 cut(s) 162, 421, 529, 637, 822, 933, 1200, 1240
Mox20I TGGCCA 2 cut(s) 59, 181
MscI TGGCCA 2 cut(s) 59, 181
MseI TTAA 2 cut(s) 846, 1005
Msp20I TGGCCA 2 cut(s) 59, 181
MspA1I CMGCKG 2 cut(s) 329, 701
MspI CCGG 3 cut(s) 37, 794, 1101
MspR9I CCNGG 5 cut(s) 645, 794, 795, 1068, 1101
MunI CAATTG 1 cut(s) 315
Mva1269I GAATGC 1 cut(s) 556
MvaI CCWGG 2 cut(s) 645, 1068
MwoI GCNNNNNNNGC 4 cut(s) 65, 178, 326, 1085
NciI CCSGG 3 cut(s) 794, 795, 1101
NcoI CCATGG 1 cut(s) 143
NdeII GATC 4 cut(s) 438, 556, 585, 1152
NlaIII CATG 7 cut(s) 50, 147, 218, 291, 450, 458, 856
NlaIV GGNNCC 4 cut(s) 41, 225, 625, 810
NmeAIII GCCGAG 2 cut(s) 192, 1105
NmuCI GTSAC 5 cut(s) 159, 470, 694, 1071, 1225
NsbI TGCGCA 2 cut(s) 213, 784
NspI RCATGY 1 cut(s) 450
PagI TCATGA 1 cut(s) 852
PaqCI CACCTGC 1 cut(s) 1084
PciI ACATGT 1 cut(s) 446
PctI GAATGC 1 cut(s) 556
PfeI GAWTC 3 cut(s) 52, 152, 1045
PflMI CCANNNNNTGG 1 cut(s) 293
PkrI GCNGC 5 cut(s) 67, 271, 328, 331, 868
PleI GAGTC 3 cut(s) 12, 893, 1258
PpsI GAGTC 3 cut(s) 12, 893, 1258
PpuMI RGGWCCY 2 cut(s) 224, 809
PscI ACATGT 1 cut(s) 446
Psp5II RGGWCCY 2 cut(s) 224, 809
Psp6I CCWGG 2 cut(s) 643, 1066
PspEI GGTNACC 1 cut(s) 1071
PspFI CCCAGC 1 cut(s) 1088
PspGI CCWGG 2 cut(s) 643, 1066
PspN4I GGNNCC 4 cut(s) 41, 225, 625, 810
PspPI GGNCC 3 cut(s) 224, 341, 809
PspPPI RGGWCCY 2 cut(s) 224, 809
PstI CTGCAG 1 cut(s) 274
PsuI RGATCY 1 cut(s) 556
PvuII CAGCTG 1 cut(s) 701
RsaI GTAC 7 cut(s) 195, 541, 767, 861, 891, 1053, 1185
RsaNI GTAC 7 cut(s) 194, 540, 766, 860, 890, 1052, 1184
SalI GTCGAC 1 cut(s) 719
SaqAI TTAA 2 cut(s) 846, 1005
SatI GCNGC 5 cut(s) 66, 270, 327, 330, 867
Sau3AI GATC 4 cut(s) 438, 556, 585, 1152
Sau96I GGNCC 3 cut(s) 224, 341, 809
ScaI AGTACT 2 cut(s) 767, 861
SchI GAGTC 3 cut(s) 13, 893, 1259
ScrFI CCNGG 5 cut(s) 645, 794, 795, 1068, 1101
SduI GDGCHC 4 cut(s) 281, 454, 626, 924
SfaNI GCATC 1 cut(s) 1152
SfcI CTRYAG 1 cut(s) 270
SinI GGWCC 3 cut(s) 224, 341, 809
SmaI CCCGGG 1 cut(s) 795
SmlI CTYRAG 1 cut(s) 628
SmoI CTYRAG 1 cut(s) 628
Sse9I AATT 9 cut(s) 106, 112, 132, 315, 589, 799, 993, 1118, 1161
SsiI CCGC 3 cut(s) 327, 724, 757
SspMI CTAG 1 cut(s) 815
StyD4I CCNGG 5 cut(s) 643, 792, 793, 1066, 1099
StyI CCWWGG 2 cut(s) 143, 953
TaaI ACNGT 3 cut(s) 544, 694, 1237
TaqI TCGA 3 cut(s) 423, 720, 1116
TaqII GACCGA 1 cut(s) 329
TasI AATT 9 cut(s) 106, 112, 132, 315, 589, 799, 993, 1118, 1161
TatI WGTACW 2 cut(s) 765, 859
TauI GCSGC 1 cut(s) 329
TfiI GAWTC 3 cut(s) 52, 152, 1045
Tru1I TTAA 2 cut(s) 846, 1005
Tru9I TTAA 2 cut(s) 846, 1005
TscAI CASTG 2 cut(s) 168, 1162
TseFI GTSAC 5 cut(s) 159, 470, 694, 1071, 1225
TseI GCWGC 4 cut(s) 65, 269, 329, 866
Tsp45I GTSAC 5 cut(s) 159, 470, 694, 1071, 1225
TspDTI ATGAA 4 cut(s) 219, 477, 869, 1011
TspGWI ACGGA 1 cut(s) 253
TspMI CCCGGG 1 cut(s) 793
TspRI CASTG 2 cut(s) 168, 1162
Van91I CCANNNNNTGG 1 cut(s) 293
VpaK11BI GGWCC 3 cut(s) 224, 341, 809
XapI RAATTY 3 cut(s) 106, 799, 1118
XceI RCATGY 1 cut(s) 450
XmaI CCCGGG 1 cut(s) 793
XmiI GTMKAC 1 cut(s) 720
XspI CTAG 1 cut(s) 815
ZrmI AGTACT 2 cut(s) 767, 861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.