MD04G1201900.v1.1

protease Do-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005022
Physical Location & Seq
Forward (+)
28810000 .. 28813232
3233 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1201900.v1.1.491

Sequence Viewer

Length: 1188 bp
ATGAACCATTTTCTGAGAAAATTTTCAACTCCGTACCGGATTGCCGCCATTGCCGCCGCTGCAGGGTCGGCTTTTTTGTTATCCGACGGCAGCAGAAACTCCGATTATACGGCATCAGTGTCAATCACTGCGCCATTGCGCGAATCCTTGCAGCTTCCATGGCAAACGGGGCTATTGGGTCCACTGTTTTCTGTGGGAACTAGTTCAGTTCCATCATCAGATATAAGGAAGGATATTTCTGGTGTTTCCCCATCCGAAGAGGCCCCTAAGCCATGTTGTGGATGTTTGGGCAGAGATTCTTTCGCAAGGGCTGCTGCAAAGGTCGGCCCTGCTGTTGTCAACATCTCTGTTCCAGAGGGTGCATATGGGATCACTCCGGGAAAGAGCATAGGCTCTGGGACTATTATTAACGAGGACGGTACAATTTTAACATGTGCTCACACTGTAGTTGATTTTTATGGCCTGAGAGCTTCATCCAAAGGGAAGGTCCATATCATCTTGCAAGATGGTAGAACATTTGAGGGTACCGTGGTCAATGCAGATTTACAATCCGATGTGGCTATAGTAAAGATAAATTCTAAAACCCCACTGCCAACGGCAAAGCTAGGCAGTTCGCGTAAACTTCAACCTGGGGATTGGGTGATAGCTAGCACTGATTTGGGTCTTGGTGGAGTGCGGAGAGAGTATTTACAGACAGATTGTGCAATCAACCCGGGAAATTCTGGGGGGCCTTTGGTCAATATTGACGGAGAAGTGATTGGTGTTAATATTATGAAAGTCTATGCTGCTGATGGATTGAGTTTTGCCTTCCCAATTGAATCAGTCACCAAAATCATGGAGCACTTCAAGAAAAGCGGGAGGGTTGTTCGGCCTTGGGTTGGACTAAAGATGGTTGATCTCACTGAGGTGATCATCACCCAGCTTAAAGAAAGAGATCCTACATTTCCAAACGTGAAGAAAGGCATTCTTGTGCCAATGGTAACTCCTGGGTCTCCTGCTGAACGAGCCGGGTTCCGTCCTGGCGACGTCGTTATTGAATTTGATGGAAACGCTGTTGGAAGCATCAAGGAGATCATTGAAATAATGGGGGACCGAGTCGGGGTGCCCATCGGGGTAGTCGTGATACGAGCGAGAGACGATATAGTGACCTTAACAGTGATCCCGGAGGAGTCCAATTCAGATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04210 ko04214 ko04215 ko05012 map04210 map04214 map04215 map05012
Pfam Domains
Protein Families
PDZ_2 Trypsin_2

Protein Analysis

396

Amino Acids

41.73

Weight (kDa)

6.47

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trypsin_2 PF13365 131 - 255 1.4e-22 Trypsin-like peptidase domain
Trypsin PF00089 207 - 274 4.3e-07 Trypsin
PDZ_2 PF13180 315 - 378 1.3e-08 PDZ domain
PDZ_6 PF17820 327 - 362 1.3e-10 PDZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1029
Acc65I GGTACC 1 cut(s) 524
AccB1I GGYRCC 2 cut(s) 524, 1102
AccB7I CCANNNNNTGG 1 cut(s) 278
AccII CGCG 2 cut(s) 141, 616
AciI CCGC 5 cut(s) 45, 54, 57, 676, 855
AclWI GGATC 3 cut(s) 377, 929, 1153
AcsI RAATTY 4 cut(s) 20, 574, 718, 1037
AcyI GRCGYC 1 cut(s) 1026
AfaI GTAC 3 cut(s) 35, 421, 526
AfiI CCNNNNNNNGG 4 cut(s) 63, 278, 878, 1099
AflIII ACRYGT 1 cut(s) 431
AgsI TTSAA 6 cut(s) 27, 626, 818, 847, 1037, 1079
AhlI ACTAGT 1 cut(s) 200
AjnI CCWGG 3 cut(s) 628, 985, 1018
AleI CACNNNNGTG 1 cut(s) 905
AluBI AGCT 5 cut(s) 154, 470, 604, 647, 922
AluI AGCT 5 cut(s) 154, 470, 604, 647, 922
Alw21I GWGCWC 2 cut(s) 439, 843
Alw26I GTCTC 2 cut(s) 996, 1128
AlwI GGATC 3 cut(s) 377, 929, 1153
Ama87I CYCGRG 1 cut(s) 712
AoxI GGCC 5 cut(s) 261, 325, 460, 728, 869
ApeKI GCWGC 6 cut(s) 59, 90, 151, 311, 314, 785
ApoI RAATTY 4 cut(s) 20, 574, 718, 1037
ArsI GACNNNNNNTTYG 2 cut(s) 471, 503
Asp700I GAANNNNTTC 2 cut(s) 22, 202
Asp718I GGTACC 1 cut(s) 524
AspLEI GCGC 2 cut(s) 133, 141
AspS9I GGNCC 6 cut(s) 179, 262, 326, 487, 728, 1090
AsuC2I CCSGG 5 cut(s) 378, 713, 714, 1009, 1163
AsuHPI GGTGA 4 cut(s) 652, 817, 907, 919
AsuNHI GCTAGC 1 cut(s) 647
AvaI CYCGRG 1 cut(s) 712
AvaII GGWCC 3 cut(s) 179, 487, 1090
BaeGI GKGCMC 1 cut(s) 1107
BaeI ACNNNNGTAYC 2 cut(s) 516, 549
BanI GGYRCC 2 cut(s) 524, 1102
Bbv12I GWGCWC 2 cut(s) 439, 843
BbvI GCAGC 6 cut(s) 46, 102, 163, 298, 301, 772
BccI CCATC 7 cut(s) 220, 259, 500, 785, 883, 1037, 1115
BceAI ACGGC 3 cut(s) 103, 126, 612
BciT130I CCWGG 3 cut(s) 630, 987, 1020
BclI TGATCA 1 cut(s) 909
BcnI CCSGG 5 cut(s) 378, 713, 714, 1009, 1163
BcoDI GTCTC 2 cut(s) 996, 1128
BcuI ACTAGT 1 cut(s) 200
BfaI CTAG 3 cut(s) 201, 605, 648
BfmI CTRYAG 3 cut(s) 60, 444, 561
BisI GCNGC 9 cut(s) 45, 54, 57, 60, 91, 152, 312, 315, 786
BlsI GCNGC 9 cut(s) 46, 55, 58, 61, 92, 153, 313, 316, 787
Bme1390I CCNGG 8 cut(s) 378, 630, 713, 714, 987, 1009, 1020, 1163
Bme18I GGWCC 3 cut(s) 179, 487, 1090
BmeT110I CYCGRG 1 cut(s) 712
BmgT120I GGNCC 6 cut(s) 179, 262, 326, 487, 728, 1090
BmiI GGNNCC 7 cut(s) 180, 264, 526, 729, 1013, 1091, 1104
BmrFI CCNGG 8 cut(s) 378, 630, 713, 714, 987, 1009, 1020, 1163
BmsI GCATC 2 cut(s) 122, 1071
BmtI GCTAGC 1 cut(s) 651
Bpu10I CCTNAGC 1 cut(s) 267
BpuMI CCSGG 5 cut(s) 378, 713, 714, 1009, 1163
BsaHI GRCGYC 1 cut(s) 1026
BsaI GGTCTC 1 cut(s) 996
BsaJI CCNNGG 6 cut(s) 158, 528, 629, 712, 872, 986
BsaWI WCCGGW 1 cut(s) 36
Bsc4I CCNNNNNNNGG 4 cut(s) 63, 278, 878, 1099
Bse3DI GCAATG 2 cut(s) 48, 134
BseBI CCWGG 3 cut(s) 630, 987, 1020
BseDI CCNNGG 6 cut(s) 158, 528, 629, 712, 872, 986
BseGI GGATG 3 cut(s) 251, 287, 473
BseLI CCNNNNNNNGG 4 cut(s) 63, 278, 878, 1099
BseMI GCAATG 2 cut(s) 48, 134
BseMII CTCAG 3 cut(s) 5, 455, 894
BseRI GAGGAG 1 cut(s) 1181
BseSI GKGCMC 1 cut(s) 1107
BseXI GCAGC 6 cut(s) 46, 102, 163, 298, 301, 772
BseYI CCCAGC 1 cut(s) 918
Bsh1236I CGCG 2 cut(s) 141, 616
BshFI GGCC 5 cut(s) 263, 327, 462, 730, 871
BshNI GGYRCC 2 cut(s) 524, 1102
BsiHKAI GWGCWC 2 cut(s) 439, 843
BsiHKCI CYCGRG 1 cut(s) 712
BsiSI CCGG 5 cut(s) 37, 377, 713, 1008, 1163
BslFI GGGAC 2 cut(s) 412, 1103
BslI CCNNNNNNNGG 4 cut(s) 63, 278, 878, 1099
BsmAI GTCTC 2 cut(s) 996, 1128
BsmBI CGTCTC 1 cut(s) 1128
BsmFI GGGAC 2 cut(s) 412, 1103
BsmI GAATGC 1 cut(s) 963
BsnI GGCC 5 cut(s) 263, 327, 462, 730, 871
Bso31I GGTCTC 1 cut(s) 996
BsoBI CYCGRG 1 cut(s) 712
Bsp1286I GDGCHC 3 cut(s) 439, 843, 1107
Bsp143I GATC 6 cut(s) 369, 895, 909, 934, 1071, 1158
Bsp19I CCATGG 1 cut(s) 158
BspACI CCGC 5 cut(s) 45, 54, 57, 676, 855
BspANI GGCC 5 cut(s) 263, 327, 462, 730, 871
BspCNI CTCAG 3 cut(s) 6, 456, 895
BspFNI CGCG 2 cut(s) 141, 616
BspLI GGNNCC 7 cut(s) 180, 264, 526, 729, 1013, 1091, 1104
BspMAI CTGCAG 1 cut(s) 64
BspOI GCTAGC 1 cut(s) 651
BspPI GGATC 3 cut(s) 377, 929, 1153
BspT107I GGYRCC 2 cut(s) 524, 1102
BspTNI GGTCTC 1 cut(s) 996
BsrDI GCAATG 2 cut(s) 48, 134
BssECI CCNNGG 6 cut(s) 158, 528, 629, 712, 872, 986
BssMI GATC 6 cut(s) 369, 895, 909, 934, 1071, 1158
BssNI GRCGYC 1 cut(s) 1026
BssT1I CCWWGG 2 cut(s) 158, 872
Bst2UI CCWGG 3 cut(s) 630, 987, 1020
Bst4CI ACNGT 5 cut(s) 186, 419, 445, 529, 1156
Bst6I CTCTTC 1 cut(s) 252
BstACI GRCGYC 1 cut(s) 1026
BstAPI GCANNNNNTGC 1 cut(s) 311
BstC8I GCNNGC 1 cut(s) 649
BstDEI CTNAG 4 cut(s) 14, 267, 464, 903
BstDSI CCRYGG 2 cut(s) 158, 528
BstF5I GGATG 3 cut(s) 251, 287, 473
BstFNI CGCG 2 cut(s) 141, 616
BstHHI GCGC 2 cut(s) 133, 141
BstKTI GATC 6 cut(s) 372, 898, 912, 937, 1074, 1161
BstMAI GTCTC 2 cut(s) 996, 1128
BstMBI GATC 6 cut(s) 369, 895, 909, 934, 1071, 1158
BstMWI GCNNNNNNNGC 8 cut(s) 50, 53, 59, 68, 160, 169, 311, 1004
BstNI CCWGG 3 cut(s) 630, 987, 1020
BstNSI RCATGY 1 cut(s) 435
BstSCI CCNGG 8 cut(s) 376, 628, 711, 712, 985, 1007, 1018, 1161
BstSFI CTRYAG 3 cut(s) 60, 444, 561
BstSLI GKGCMC 1 cut(s) 1107
BstUI CGCG 2 cut(s) 141, 616
BstV1I GCAGC 6 cut(s) 46, 102, 163, 298, 301, 772
BstX2I RGATCY 1 cut(s) 934
BstXI CCANNNNNNTGG 1 cut(s) 835
BstYI RGATCY 1 cut(s) 934
BsuRI GGCC 5 cut(s) 263, 327, 462, 730, 871
BtgI CCRYGG 2 cut(s) 158, 528
BtsCI GGATG 3 cut(s) 251, 287, 473
BtsI GCAGTG 2 cut(s) 126, 587
BtsIMutI CAGTG 8 cut(s) 123, 126, 182, 441, 587, 651, 900, 1161
Cac8I GCNNGC 1 cut(s) 649
CfoI GCGC 2 cut(s) 133, 141
Cfr13I GGNCC 6 cut(s) 179, 262, 326, 487, 728, 1090
Cfr9I CCCGGG 1 cut(s) 712
Csp6I GTAC 3 cut(s) 34, 420, 525
CviAII CATG 4 cut(s) 159, 273, 432, 835
CviQI GTAC 3 cut(s) 34, 420, 525
DdeI CTNAG 4 cut(s) 14, 267, 464, 903
DpnI GATC 6 cut(s) 371, 897, 911, 936, 1073, 1160
DpnII GATC 6 cut(s) 369, 895, 909, 934, 1071, 1158
Eam1104I CTCTTC 1 cut(s) 252
EarI CTCTTC 1 cut(s) 252
Eco130I CCWWGG 2 cut(s) 158, 872
Eco31I GGTCTC 1 cut(s) 996
Eco47I GGWCC 3 cut(s) 179, 487, 1090
Eco88I CYCGRG 1 cut(s) 712
EcoO109I RGGNCCY 2 cut(s) 262, 728
EcoRII CCWGG 3 cut(s) 628, 985, 1018
EcoT14I CCWWGG 2 cut(s) 158, 872
ErhI CCWWGG 2 cut(s) 158, 872
Esp3I CGTCTC 1 cut(s) 1128
FaeI CATG 4 cut(s) 162, 276, 435, 838
FalI AAGNNNNNCTT 2 cut(s) 951, 983
FaqI GGGAC 2 cut(s) 412, 1103
FatI CATG 4 cut(s) 158, 272, 431, 834
FauI CCCGC 1 cut(s) 848
FauNDI CATATG 1 cut(s) 364
FbaI TGATCA 1 cut(s) 909
Fnu4HI GCNGC 9 cut(s) 45, 54, 57, 60, 91, 152, 312, 315, 786
FokI GGATG 3 cut(s) 238, 294, 460
Fsp4HI GCNGC 9 cut(s) 45, 54, 57, 60, 91, 152, 312, 315, 786
FspBI CTAG 3 cut(s) 201, 605, 648
GlaI GCGC 2 cut(s) 132, 140
GluI GCNGC 9 cut(s) 45, 54, 57, 60, 91, 152, 312, 315, 786
GsaI CCCAGC 1 cut(s) 922
HaeIII GGCC 5 cut(s) 263, 327, 462, 730, 871
HapII CCGG 5 cut(s) 37, 377, 713, 1008, 1163
HhaI GCGC 2 cut(s) 133, 141
Hin1I GRCGYC 1 cut(s) 1026
Hin1II CATG 4 cut(s) 162, 276, 435, 838
Hin6I GCGC 2 cut(s) 131, 139
HinP1I GCGC 2 cut(s) 131, 139
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 5 cut(s) 143, 296, 818, 1095, 1169
HpaII CCGG 5 cut(s) 37, 377, 713, 1008, 1163
HphI GGTGA 4 cut(s) 652, 817, 907, 919
Hpy166II GTNNAC 3 cut(s) 182, 340, 620
Hpy188I TCNGA 7 cut(s) 15, 85, 103, 220, 256, 553, 1180
Hpy188III TCNNGA 3 cut(s) 353, 847, 1120
Hpy8I GTNNAC 3 cut(s) 182, 340, 620
Hpy99I CGWCG 3 cut(s) 89, 1028, 1031
HpyAV CCTTC 3 cut(s) 223, 478, 817
HpyCH4III ACNGT 5 cut(s) 186, 419, 445, 529, 1156
HpyCH4IV ACGT 2 cut(s) 951, 1026
HpyCH4V TGCA 7 cut(s) 62, 151, 317, 362, 502, 539, 704
HpyF10VI GCNNNNNNNGC 8 cut(s) 50, 53, 59, 68, 160, 169, 311, 1004
HpyF3I CTNAG 4 cut(s) 14, 267, 464, 903
HpySE526I ACGT 2 cut(s) 951, 1026
Hsp92I GRCGYC 1 cut(s) 1026
Hsp92II CATG 4 cut(s) 162, 276, 435, 838
HspAI GCGC 2 cut(s) 131, 139
KpnI GGTACC 1 cut(s) 528
Ksp22I TGATCA 1 cut(s) 909
Kzo9I GATC 6 cut(s) 369, 895, 909, 934, 1071, 1158
LmnI GCTCC 1 cut(s) 838
Lsp1109I GCAGC 6 cut(s) 46, 102, 163, 298, 301, 772
LweI GCATC 2 cut(s) 122, 1071
MaeI CTAG 3 cut(s) 201, 605, 648
MaeII ACGT 2 cut(s) 951, 1026
MaeIII GTNAC 3 cut(s) 823, 979, 1144
MalI GATC 6 cut(s) 371, 897, 911, 936, 1073, 1160
MboI GATC 6 cut(s) 369, 895, 909, 934, 1071, 1158
MboII GAAGA 2 cut(s) 269, 967
MfeI CAATTG 1 cut(s) 813
MflI RGATCY 1 cut(s) 934
MhlI GDGCHC 3 cut(s) 439, 843, 1107
MluCI AATT 7 cut(s) 20, 423, 574, 718, 813, 1037, 1174
MlyI GAGTC 2 cut(s) 1104, 1178
MmeI TCCRAC 3 cut(s) 108, 859, 1036
MnlI CCTC 7 cut(s) 253, 349, 406, 514, 852, 898, 1159
MroXI GAANNNNTTC 2 cut(s) 22, 202
MseI TTAA 5 cut(s) 408, 428, 765, 924, 1151
MslI CAYNNNNRTG 2 cut(s) 905, 968
MspA1I CMGCKG 1 cut(s) 59
MspI CCGG 5 cut(s) 37, 377, 713, 1008, 1163
MspR9I CCNGG 8 cut(s) 378, 630, 713, 714, 987, 1009, 1020, 1163
MunI CAATTG 1 cut(s) 813
Mva1269I GAATGC 1 cut(s) 963
MvaI CCWGG 3 cut(s) 630, 987, 1020
MvnI CGCG 2 cut(s) 141, 616
MwoI GCNNNNNNNGC 8 cut(s) 50, 53, 59, 68, 160, 169, 311, 1004
NciI CCSGG 5 cut(s) 378, 713, 714, 1009, 1163
NcoI CCATGG 1 cut(s) 158
NdeI CATATG 1 cut(s) 364
NdeII GATC 6 cut(s) 369, 895, 909, 934, 1071, 1158
NheI GCTAGC 1 cut(s) 647
NlaIII CATG 4 cut(s) 162, 276, 435, 838
NlaIV GGNNCC 7 cut(s) 180, 264, 526, 729, 1013, 1091, 1104
NmuCI GTSAC 2 cut(s) 823, 1144
NspI RCATGY 1 cut(s) 435
OliI CACNNNNGTG 1 cut(s) 905
PciI ACATGT 1 cut(s) 431
PcsI WCGNNNNNNNCGW 1 cut(s) 1116
PctI GAATGC 1 cut(s) 963
PdmI GAANNNNTTC 2 cut(s) 22, 202
PfeI GAWTC 3 cut(s) 143, 296, 818
PflFI GACNNNGTC 1 cut(s) 1094
PflMI CCANNNNNTGG 1 cut(s) 278
PfoI TCCNGGA 2 cut(s) 376, 1161
PkrI GCNGC 9 cut(s) 46, 55, 58, 61, 92, 153, 313, 316, 787
PleI GAGTC 2 cut(s) 1103, 1177
PpsI GAGTC 2 cut(s) 1103, 1177
PscI ACATGT 1 cut(s) 431
Psp6I CCWGG 3 cut(s) 628, 985, 1018
PspFI CCCAGC 1 cut(s) 918
PspGI CCWGG 3 cut(s) 628, 985, 1018
PspN4I GGNNCC 7 cut(s) 180, 264, 526, 729, 1013, 1091, 1104
PspPI GGNCC 6 cut(s) 179, 262, 326, 487, 728, 1090
PstI CTGCAG 1 cut(s) 64
PsuI RGATCY 1 cut(s) 934
PsyI GACNNNGTC 1 cut(s) 1094
RsaI GTAC 3 cut(s) 35, 421, 526
RsaNI GTAC 3 cut(s) 34, 420, 525
RseI CAYNNNNRTG 2 cut(s) 905, 968
SaqAI TTAA 5 cut(s) 408, 428, 765, 924, 1151
SatI GCNGC 9 cut(s) 45, 54, 57, 60, 91, 152, 312, 315, 786
Sau3AI GATC 6 cut(s) 369, 895, 909, 934, 1071, 1158
Sau96I GGNCC 6 cut(s) 179, 262, 326, 487, 728, 1090
SchI GAGTC 2 cut(s) 1104, 1178
ScrFI CCNGG 8 cut(s) 378, 630, 713, 714, 987, 1009, 1020, 1163
SduI GDGCHC 3 cut(s) 439, 843, 1107
SfaNI GCATC 2 cut(s) 122, 1071
SfcI CTRYAG 3 cut(s) 60, 444, 561
SinI GGWCC 3 cut(s) 179, 487, 1090
SmaI CCCGGG 1 cut(s) 714
SmiMI CAYNNNNRTG 2 cut(s) 905, 968
SpeI ACTAGT 1 cut(s) 200
Sse9I AATT 7 cut(s) 20, 423, 574, 718, 813, 1037, 1174
SsiI CCGC 5 cut(s) 45, 54, 57, 676, 855
SspI AATATT 2 cut(s) 742, 769
SspMI CTAG 3 cut(s) 201, 605, 648
StyD4I CCNGG 8 cut(s) 376, 628, 711, 712, 985, 1007, 1018, 1161
StyI CCWWGG 2 cut(s) 158, 872
TaaI ACNGT 5 cut(s) 186, 419, 445, 529, 1156
TaiI ACGT 2 cut(s) 954, 1029
TaqII GACCGA 1 cut(s) 1107
TasI AATT 7 cut(s) 20, 423, 574, 718, 813, 1037, 1174
TauI GCSGC 3 cut(s) 47, 56, 59
TfiI GAWTC 3 cut(s) 143, 296, 818
Tru1I TTAA 5 cut(s) 408, 428, 765, 924, 1151
Tru9I TTAA 5 cut(s) 408, 428, 765, 924, 1151
TscAI CASTG 8 cut(s) 123, 133, 189, 448, 594, 658, 907, 1161
TseFI GTSAC 2 cut(s) 823, 1144
TseI GCWGC 6 cut(s) 59, 90, 151, 311, 314, 785
Tsp45I GTSAC 2 cut(s) 823, 1144
TspDTI ATGAA 3 cut(s) 17, 462, 788
TspGWI ACGGA 3 cut(s) 21, 762, 1004
TspMI CCCGGG 1 cut(s) 712
TspRI CASTG 8 cut(s) 123, 133, 189, 448, 594, 658, 907, 1161
Tth111I GACNNNGTC 1 cut(s) 1094
Van91I CCANNNNNTGG 1 cut(s) 278
VpaK11BI GGWCC 3 cut(s) 179, 487, 1090
XapI RAATTY 4 cut(s) 20, 574, 718, 1037
XceI RCATGY 1 cut(s) 435
XmaI CCCGGG 1 cut(s) 712
XmnI GAANNNNTTC 2 cut(s) 22, 202
XspI CTAG 3 cut(s) 201, 605, 648
ZraI GACGTC 1 cut(s) 1027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.