Rw3G005350

protease Do-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0005022
Physical Location & Seq
Reverse (-)
4450933 .. 4454233
3301 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G005350.1

Sequence Viewer

Length: 1233 bp
ATGAGTCATTTTCTGAGGAAGGTTTTGATTGCAAACCGGAATCGACATCGGATTCTGGCCATTGCTGCCGTTGGTTCGGCTTTTTTGTATGCCAGAGGGGACGAGAATTTCTATTCGTCATTCTCGGTGTCAGTTCCTGCACCATGGCGTGAATCGTTGTCACGGCCGAGGCAAAACTGGCCATTTGTTGTGCCATTATTTTCTGTTGCACATGGTTCGGGTCCTTCATCGGATGTATCTGGTTTCTCCGTAGCTCCAGAGAGCCCAAAGCCATGCTGTGGATGTTTGGGCAAAGATACAATTGCAAAGGCAGCTGCGAAGGTCGGTCCTGCAGTTGTCAATATATCTCTTCAACAGGGTATATATGGGGTTGGTGTTGGAAAGGGTATAGGCTCTGGAACTATAATCGATGAGGATGGCACGATCCTAACATGTGCTCATGCTGTGGTTGATTTTCACGGGCTGAGAGCTTCATCAAAGGGGAAGGTTGGTGTAACTTTGCAAGATGGTCGGACATTTGAGGGTACTGTGGTGAATGCAGATCTACAATCTGATGTTGCTATTGTGAAGATCAATTCTAAAACCCCCCTTCCAACAGCAAAGTTGGGCACCTCAAGCAAACTCCAACCTGGGGATTGGGTGATAGCAGTTGGCTGTCCACTTTCGCTTCAGAATACCGTCACAGCTGGCATTGTAAGTTGTGTCGACCGCAAAAGTAGTGATTTGGGTCTTGGTGGACTGCGGAGAGAGTACTTACAGACAGATTGCGCAATCAACCCGGGGAATTCTGGGGGACCTCTAGTAAATATGGATGGAGAAGTTATTGGTGTTAATATCATGAAAGTACTTGCTGCCGATGGATTGAGTTTTGCTGTACCGATTGACTCAATCGCCAAGATAATGGAGCACTTTAGTAAAAATGGGAGGGTTGTTCGGCCTTGGCTTGGACTGAAGATGATTGACCTAAATGATATGATAATTTCTCAGCTTAAAGACAGAGATGGTTCATTTCCAAATATAAAAAGAGGGATTCTCGTACCTATGGTAACGCCTGGGTCACCTGCTGAGCGTGCTGGGTTTCGTCCCGGTGATGTTGTTGTCGAATTTGATGGAAAGCCTGTTGCAAGCATCACAGAGATCACTGAAATTATGGGAGATAGAGTTGGAGTACCATTGAAGGTGGTTGTGAAGAGGGCGAAAGATGAGAACAACGGCAGTAGTACCATTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04210 ko04214 ko04215 ko05012 map04210 map04214 map04215 map05012
Pfam Domains
Protein Families
PDZ_2 Trypsin_2

Protein Analysis

410

Amino Acids

43.26

Weight (kDa)

9.0

Isoelectric Point (pI)

36.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trypsin PF00089 108 - 299 2.5e-18 Trypsin
Trypsin_2 PF13365 131 - 277 1e-33 Trypsin-like peptidase domain
PDZ_2 PF13180 324 - 403 1.1e-10 PDZ domain
PDZ_6 PF17820 349 - 396 4.2e-11 PDZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1069
Acc16I TGCGCA 1 cut(s) 769
Acc36I ACCTGC 1 cut(s) 1069
AccB1I GGYRCC 1 cut(s) 608
AccB7I CCANNNNNTGG 1 cut(s) 278
AccI GTMKAC 1 cut(s) 705
AciI CCGC 2 cut(s) 709, 742
AclWI GGATC 1 cut(s) 418
AcoI YGGCCR 3 cut(s) 57, 164, 179
AcsI RAATTY 3 cut(s) 106, 784, 1103
AcuI CTGAAG 2 cut(s) 653, 971
AfaI GTAC 7 cut(s) 526, 752, 846, 876, 1038, 1170, 1222
AfiI CCNNNNNNNGG 3 cut(s) 278, 631, 944
AflIII ACRYGT 1 cut(s) 431
AgsI TTSAA 2 cut(s) 353, 1177
AjnI CCWGG 2 cut(s) 628, 1051
AluBI AGCT 5 cut(s) 254, 314, 470, 686, 988
AluI AGCT 5 cut(s) 254, 314, 470, 686, 988
Alw21I GWGCWC 2 cut(s) 439, 909
AlwI GGATC 1 cut(s) 418
AlwNI CAGNNNCTG 1 cut(s) 137
Ama87I CYCGRG 1 cut(s) 778
AoxI GGCC 4 cut(s) 57, 164, 179, 935
ApeKI GCWGC 4 cut(s) 65, 311, 314, 851
ApoI RAATTY 3 cut(s) 106, 784, 1103
AspLEI GCGC 1 cut(s) 770
AspS9I GGNCC 3 cut(s) 221, 326, 794
AsuC2I CCSGG 3 cut(s) 779, 780, 1086
AsuHPI GGTGA 4 cut(s) 544, 652, 1050, 1100
AvaI CYCGRG 1 cut(s) 778
AvaII GGWCC 3 cut(s) 221, 326, 794
BaeGI GKGCMC 1 cut(s) 611
BaeI ACNNNNGTAYC 2 cut(s) 516, 549
BalI TGGCCA 2 cut(s) 59, 181
BanI GGYRCC 1 cut(s) 608
BanII GRGCYC 1 cut(s) 266
Bbv12I GWGCWC 2 cut(s) 439, 909
BbvI GCAGC 4 cut(s) 52, 301, 323, 838
BccI CCATC 6 cut(s) 410, 500, 806, 851, 995, 1103
BceAI ACGGC 3 cut(s) 53, 179, 1228
BcgI CGANNNNNNTGC 4 cut(s) 198, 232, 491, 525
BciT130I CCWGG 2 cut(s) 630, 1053
BcnI CCSGG 3 cut(s) 779, 780, 1086
BfaI CTAG 1 cut(s) 800
BfmI CTRYAG 1 cut(s) 330
BfuAI ACCTGC 1 cut(s) 1069
BglII AGATCT 1 cut(s) 541
BisI GCNGC 4 cut(s) 66, 312, 315, 852
BlpI GCTNAGC 1 cut(s) 1065
BlsI GCNGC 4 cut(s) 67, 313, 316, 853
BmcAI AGTACT 2 cut(s) 752, 846
Bme1390I CCNGG 5 cut(s) 630, 779, 780, 1053, 1086
Bme18I GGWCC 3 cut(s) 221, 326, 794
BmeT110I CYCGRG 1 cut(s) 778
BmgT120I GGNCC 3 cut(s) 221, 326, 794
BmiI GGNNCC 3 cut(s) 222, 610, 795
BmrFI CCNGG 5 cut(s) 630, 779, 780, 1053, 1086
BmsI GCATC 1 cut(s) 1137
BplI GAGNNNNNCTC 2 cut(s) 1017, 1049
BpmI CTGGAG 1 cut(s) 240
Bpu1102I GCTNAGC 1 cut(s) 1065
BpuEI CTTGAG 1 cut(s) 598
BpuMI CCSGG 3 cut(s) 779, 780, 1086
Bsa29I ATCGAT 1 cut(s) 408
BsaJI CCNNGG 7 cut(s) 143, 167, 629, 778, 779, 938, 1052
BsaWI WCCGGW 1 cut(s) 36
BsaXI ACNNNNNCTCC 2 cut(s) 1146, 1176
Bsc4I CCNNNNNNNGG 3 cut(s) 278, 631, 944
Bse1I ACTGG 1 cut(s) 182
Bse3DI GCAATG 1 cut(s) 60
BseBI CCWGG 2 cut(s) 630, 1053
BseCI ATCGAT 1 cut(s) 408
BseDI CCNNGG 7 cut(s) 143, 167, 629, 778, 779, 938, 1052
BseGI GGATG 4 cut(s) 238, 287, 421, 817
BseLI CCNNNNNNNGG 3 cut(s) 278, 631, 944
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 4 cut(s) 5, 455, 998, 1056
BseNI ACTGG 1 cut(s) 182
BseSI GKGCMC 1 cut(s) 611
BseX3I CGGCCG 1 cut(s) 164
BseXI GCAGC 4 cut(s) 52, 301, 323, 838
BseYI CCCAGC 1 cut(s) 1073
BsgI GTGCAG 1 cut(s) 123
Bsh1285I CGRYCG 2 cut(s) 167, 709
BshFI GGCC 4 cut(s) 59, 166, 181, 937
BshNI GGYRCC 1 cut(s) 608
BshVI ATCGAT 1 cut(s) 408
BsiEI CGRYCG 2 cut(s) 167, 709
BsiHKAI GWGCWC 2 cut(s) 439, 909
BsiHKCI CYCGRG 1 cut(s) 778
BsiSI CCGG 3 cut(s) 37, 779, 1086
BslFI GGGAC 3 cut(s) 113, 807, 1068
BslI CCNNNNNNNGG 3 cut(s) 278, 631, 944
BsmFI GGGAC 3 cut(s) 113, 807, 1068
BsmI GAATGC 1 cut(s) 541
BsnI GGCC 4 cut(s) 59, 166, 181, 937
BsoBI CYCGRG 1 cut(s) 778
Bsp1286I GDGCHC 4 cut(s) 266, 439, 611, 909
Bsp143I GATC 4 cut(s) 423, 541, 570, 1137
Bsp1720I GCTNAGC 1 cut(s) 1065
Bsp19I CCATGG 1 cut(s) 143
BspACI CCGC 2 cut(s) 709, 742
BspANI GGCC 4 cut(s) 59, 166, 181, 937
BspCNI CTCAG 4 cut(s) 6, 456, 997, 1057
BspDI ATCGAT 1 cut(s) 408
BspHI TCATGA 1 cut(s) 837
BspLI GGNNCC 3 cut(s) 222, 610, 795
BspMAI CTGCAG 1 cut(s) 334
BspMI ACCTGC 1 cut(s) 1069
BspPI GGATC 1 cut(s) 418
BspT107I GGYRCC 1 cut(s) 608
BsrDI GCAATG 1 cut(s) 60
BsrI ACTGG 1 cut(s) 182
BssECI CCNNGG 7 cut(s) 143, 167, 629, 778, 779, 938, 1052
BssMI GATC 4 cut(s) 423, 541, 570, 1137
BssT1I CCWWGG 2 cut(s) 143, 938
Bst2UI CCWGG 2 cut(s) 630, 1053
Bst4CI ACNGT 2 cut(s) 529, 679
Bst6I CTCTTC 2 cut(s) 354, 1184
BstC8I GCNNGC 3 cut(s) 688, 1071, 1126
BstDEI CTNAG 4 cut(s) 14, 464, 984, 1065
BstDSI CCRYGG 1 cut(s) 143
BstEII GGTNACC 1 cut(s) 1056
BstF5I GGATG 4 cut(s) 238, 287, 421, 817
BstHHI GCGC 1 cut(s) 770
BstKTI GATC 4 cut(s) 426, 544, 573, 1140
BstMBI GATC 4 cut(s) 423, 541, 570, 1137
BstMCI CGRYCG 2 cut(s) 167, 709
BstMWI GCNNNNNNNGC 5 cut(s) 65, 178, 311, 615, 1070
BstNI CCWGG 2 cut(s) 630, 1053
BstNSI RCATGY 1 cut(s) 435
BstPI GGTNACC 1 cut(s) 1056
BstSCI CCNGG 5 cut(s) 628, 777, 778, 1051, 1084
BstSFI CTRYAG 1 cut(s) 330
BstSLI GKGCMC 1 cut(s) 611
BstV1I GCAGC 4 cut(s) 52, 301, 323, 838
BstX2I RGATCY 1 cut(s) 541
BstXI CCANNNNNNTGG 1 cut(s) 901
BstYI RGATCY 1 cut(s) 541
BstZI CGGCCG 1 cut(s) 164
Bsu15I ATCGAT 1 cut(s) 408
BsuRI GGCC 4 cut(s) 59, 166, 181, 937
BsuTUI ATCGAT 1 cut(s) 408
BtgI CCRYGG 1 cut(s) 143
BtsCI GGATG 4 cut(s) 238, 287, 421, 817
BtsIMutI CAGTG 1 cut(s) 1140
BveI ACCTGC 1 cut(s) 1069
Cac8I GCNNGC 3 cut(s) 688, 1071, 1126
CaiI CAGNNNCTG 1 cut(s) 137
CciI TCATGA 1 cut(s) 837
CfoI GCGC 1 cut(s) 770
Cfr13I GGNCC 3 cut(s) 221, 326, 794
Cfr9I CCCGGG 1 cut(s) 778
ClaI ATCGAT 1 cut(s) 408
Csp6I GTAC 7 cut(s) 525, 751, 845, 875, 1037, 1169, 1221
CviAII CATG 6 cut(s) 144, 212, 273, 432, 440, 838
CviQI GTAC 7 cut(s) 525, 751, 845, 875, 1037, 1169, 1221
DdeI CTNAG 4 cut(s) 14, 464, 984, 1065
DpnI GATC 4 cut(s) 425, 543, 572, 1139
DpnII GATC 4 cut(s) 423, 541, 570, 1137
EaeI YGGCCR 3 cut(s) 57, 164, 179
EagI CGGCCG 1 cut(s) 164
Eam1104I CTCTTC 2 cut(s) 354, 1184
EarI CTCTTC 2 cut(s) 354, 1184
EclXI CGGCCG 1 cut(s) 164
Eco130I CCWWGG 2 cut(s) 143, 938
Eco24I GRGCYC 1 cut(s) 266
Eco47I GGWCC 3 cut(s) 221, 326, 794
Eco52I CGGCCG 1 cut(s) 164
Eco57I CTGAAG 2 cut(s) 653, 971
Eco88I CYCGRG 1 cut(s) 778
Eco91I GGTNACC 1 cut(s) 1056
EcoO109I RGGNCCY 2 cut(s) 221, 794
EcoO65I GGTNACC 1 cut(s) 1056
EcoRI GAATTC 1 cut(s) 784
EcoRII CCWGG 2 cut(s) 628, 1051
EcoT14I CCWWGG 2 cut(s) 143, 938
EcoT38I GRGCYC 1 cut(s) 266
ErhI CCWWGG 2 cut(s) 143, 938
FaeI CATG 6 cut(s) 147, 215, 276, 435, 443, 841
FaqI GGGAC 3 cut(s) 113, 807, 1068
FatI CATG 6 cut(s) 143, 211, 272, 431, 439, 837
FblI GTMKAC 1 cut(s) 705
Fnu4HI GCNGC 4 cut(s) 66, 312, 315, 852
FokI GGATG 4 cut(s) 245, 294, 428, 824
FriOI GRGCYC 1 cut(s) 266
Fsp4HI GCNGC 4 cut(s) 66, 312, 315, 852
FspBI CTAG 1 cut(s) 800
FspI TGCGCA 1 cut(s) 769
GlaI GCGC 1 cut(s) 769
GluI GCNGC 4 cut(s) 66, 312, 315, 852
GsaI CCCAGC 1 cut(s) 1077
GsuI CTGGAG 1 cut(s) 240
HaeIII GGCC 4 cut(s) 59, 166, 181, 937
HapII CCGG 3 cut(s) 37, 779, 1086
HhaI GCGC 1 cut(s) 770
Hin1II CATG 6 cut(s) 147, 215, 276, 435, 443, 841
Hin6I GCGC 1 cut(s) 768
HinP1I GCGC 1 cut(s) 768
HincII GTYRAC 1 cut(s) 706
HindII GTYRAC 1 cut(s) 706
HinfI GANTC 6 cut(s) 4, 40, 52, 152, 884, 1030
HpaII CCGG 3 cut(s) 37, 779, 1086
HphI GGTGA 4 cut(s) 544, 652, 1050, 1100
Hpy166II GTNNAC 3 cut(s) 659, 706, 737
Hpy188I TCNGA 7 cut(s) 15, 51, 232, 513, 553, 672, 1232
Hpy188III TCNNGA 3 cut(s) 257, 396, 838
Hpy8I GTNNAC 3 cut(s) 659, 706, 737
HpyAV CCTTC 6 cut(s) 13, 234, 313, 478, 599, 1171
HpyCH4III ACNGT 2 cut(s) 529, 679
HpyCH4V TGCA 8 cut(s) 32, 140, 209, 305, 332, 502, 539, 1124
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 178, 311, 615, 1070
HpyF3I CTNAG 4 cut(s) 14, 464, 984, 1065
Hsp92II CATG 6 cut(s) 147, 215, 276, 435, 443, 841
HspAI GCGC 1 cut(s) 768
Kzo9I GATC 4 cut(s) 423, 541, 570, 1137
LmnI GCTCC 2 cut(s) 259, 904
Lsp1109I GCAGC 4 cut(s) 52, 301, 323, 838
LweI GCATC 1 cut(s) 1137
MaeI CTAG 1 cut(s) 800
MaeIII GTNAC 5 cut(s) 159, 493, 679, 1045, 1056
MalI GATC 4 cut(s) 425, 543, 572, 1139
MboI GATC 4 cut(s) 423, 541, 570, 1137
MboII GAAGA 4 cut(s) 341, 580, 964, 1201
MfeI CAATTG 1 cut(s) 300
MflI RGATCY 1 cut(s) 541
MhlI GDGCHC 4 cut(s) 266, 439, 611, 909
MlsI TGGCCA 2 cut(s) 59, 181
MluCI AATT 7 cut(s) 106, 300, 574, 784, 978, 1103, 1146
MluNI TGGCCA 2 cut(s) 59, 181
MlyI GAGTC 2 cut(s) 13, 878
MmeI TCCRAC 5 cut(s) 358, 491, 617, 649, 1144
Mox20I TGGCCA 2 cut(s) 59, 181
MscI TGGCCA 2 cut(s) 59, 181
MseI TTAA 2 cut(s) 831, 990
Msp20I TGGCCA 2 cut(s) 59, 181
MspA1I CMGCKG 2 cut(s) 314, 686
MspI CCGG 3 cut(s) 37, 779, 1086
MspR9I CCNGG 5 cut(s) 630, 779, 780, 1053, 1086
MunI CAATTG 1 cut(s) 300
Mva1269I GAATGC 1 cut(s) 541
MvaI CCWGG 2 cut(s) 630, 1053
MwoI GCNNNNNNNGC 5 cut(s) 65, 178, 311, 615, 1070
NciI CCSGG 3 cut(s) 779, 780, 1086
NcoI CCATGG 1 cut(s) 143
NdeII GATC 4 cut(s) 423, 541, 570, 1137
NlaIII CATG 6 cut(s) 147, 215, 276, 435, 443, 841
NlaIV GGNNCC 3 cut(s) 222, 610, 795
NmeAIII GCCGAG 1 cut(s) 192
NmuCI GTSAC 3 cut(s) 159, 679, 1056
NsbI TGCGCA 1 cut(s) 769
NspI RCATGY 1 cut(s) 435
PagI TCATGA 1 cut(s) 837
PaqCI CACCTGC 1 cut(s) 1069
PciI ACATGT 1 cut(s) 431
PctI GAATGC 1 cut(s) 541
PfeI GAWTC 4 cut(s) 40, 52, 152, 1030
PflMI CCANNNNNTGG 1 cut(s) 278
PkrI GCNGC 4 cut(s) 67, 313, 316, 853
PleI GAGTC 2 cut(s) 12, 878
PpsI GAGTC 2 cut(s) 12, 878
PpuMI RGGWCCY 2 cut(s) 221, 794
PscI ACATGT 1 cut(s) 431
Psp5II RGGWCCY 2 cut(s) 221, 794
Psp6I CCWGG 2 cut(s) 628, 1051
PspEI GGTNACC 1 cut(s) 1056
PspFI CCCAGC 1 cut(s) 1073
PspGI CCWGG 2 cut(s) 628, 1051
PspN4I GGNNCC 3 cut(s) 222, 610, 795
PspPI GGNCC 3 cut(s) 221, 326, 794
PspPPI RGGWCCY 2 cut(s) 221, 794
PstI CTGCAG 1 cut(s) 334
PstNI CAGNNNCTG 1 cut(s) 137
PsuI RGATCY 1 cut(s) 541
PvuII CAGCTG 2 cut(s) 314, 686
RsaI GTAC 7 cut(s) 526, 752, 846, 876, 1038, 1170, 1222
RsaNI GTAC 7 cut(s) 525, 751, 845, 875, 1037, 1169, 1221
SalI GTCGAC 1 cut(s) 704
SaqAI TTAA 2 cut(s) 831, 990
SatI GCNGC 4 cut(s) 66, 312, 315, 852
Sau3AI GATC 4 cut(s) 423, 541, 570, 1137
Sau96I GGNCC 3 cut(s) 221, 326, 794
ScaI AGTACT 2 cut(s) 752, 846
SchI GAGTC 2 cut(s) 13, 878
ScrFI CCNGG 5 cut(s) 630, 779, 780, 1053, 1086
SduI GDGCHC 4 cut(s) 266, 439, 611, 909
SfaNI GCATC 1 cut(s) 1137
SfcI CTRYAG 1 cut(s) 330
SinI GGWCC 3 cut(s) 221, 326, 794
SmaI CCCGGG 1 cut(s) 780
SmlI CTYRAG 1 cut(s) 613
SmoI CTYRAG 1 cut(s) 613
Sse9I AATT 7 cut(s) 106, 300, 574, 784, 978, 1103, 1146
SsiI CCGC 2 cut(s) 709, 742
SspMI CTAG 1 cut(s) 800
StyD4I CCNGG 5 cut(s) 628, 777, 778, 1051, 1084
StyI CCWWGG 2 cut(s) 143, 938
TaaI ACNGT 2 cut(s) 529, 679
TaqI TCGA 4 cut(s) 43, 408, 705, 1101
TaqII GACCGA 1 cut(s) 314
TasI AATT 7 cut(s) 106, 300, 574, 784, 978, 1103, 1146
TatI WGTACW 2 cut(s) 750, 844
TfiI GAWTC 4 cut(s) 40, 52, 152, 1030
Tru1I TTAA 2 cut(s) 831, 990
Tru9I TTAA 2 cut(s) 831, 990
TscAI CASTG 1 cut(s) 1147
TseFI GTSAC 3 cut(s) 159, 679, 1056
TseI GCWGC 4 cut(s) 65, 311, 314, 851
Tsp45I GTSAC 3 cut(s) 159, 679, 1056
TspDTI ATGAA 4 cut(s) 216, 462, 854, 996
TspGWI ACGGA 1 cut(s) 238
TspMI CCCGGG 1 cut(s) 778
TspRI CASTG 1 cut(s) 1147
Van91I CCANNNNNTGG 1 cut(s) 278
VpaK11BI GGWCC 3 cut(s) 221, 326, 794
XapI RAATTY 3 cut(s) 106, 784, 1103
XceI RCATGY 1 cut(s) 435
XmaI CCCGGG 1 cut(s) 778
XmiI GTMKAC 1 cut(s) 705
XspI CTAG 1 cut(s) 800
ZrmI AGTACT 2 cut(s) 752, 846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.