pycom04g17810

protease Do-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005022
Physical Location & Seq
Forward (+)
20051310 .. 20054218
2909 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g17810.5

Sequence Viewer

Length: 1251 bp
ATGAACCATTTTCTGAAAAATTCTACTCCGCACCGGATTGCCGCCATTGCCGCCGCTGCAGGGTCGGCTTATTTGTTATCCGACGGCAGCAGAAACTCCGATTATACGGCATCAGTGTCAATCACTGCACCATTGCGCGAATCCTTGCAGCTTCCATGGCAAACGGGGCTATTGGCTCCACTGTTTTCTGTGGGAACTAGTTCAATTCCATCATCAGATATAAGGAAGGATATTTCTGGTGTTTCCCCTTCTGAAGAGGCCCCTAAGCCATGTTCTGGATGTTTGAGCAGGGATTCTTTCGCAAAGGCTGCTGCGAAGGTTGGTCCTGCTGTTGTCAACATCTCTGCTCCAGAGGGTGCATATGGGATCACTCCGGGAAAGAGTATAGGCTCTGGGACGATTATTGATGAGGACGGTACAATTTTAACATGCGCTCATACTGTAGTTGATTTTTATGGCCTGAGAGCTTCATCAAAAGGGAAGGTTCATGTCACCTTGCAAGATGGTAGAACATTTGAGGGTACCGTGGTCAATGCAGATTTACAATCCGATGTGGCTATAGTAAAGATAAATTCTAAAACCCCACTGCCAACAGCAAAGCTAGGCAGTTCGCGTAAACTTCAACCTGGGGATTGGGTGATAGCTGTTGGCTGTCCACTTTCACTTCAGAATACTGTCACAGCTGGTATCGTAAGTTGTGTTGACCGAAAGAGCACTGATTTGGGTCTTGGTGGAGTGCGGAGAGAGTATTTACAGACAGATTGCGCAATCAACCCGGGAAATTCTGGGGGGCCTTTGGTCAATATTGACGGAGAAGTGATTGGTGTTAATATTATGAAAGTCTATGCTGCTGATGGATTGAGTTTTGCCTTCCCAATTGATTCAGTCACCAAAATCATGGAGCACTTCAAGAAAAGTGGGAGGGTTGTTCGGCCTTGGGTTGGACTAAAGATGGTTGATCTCACTGAGGTCATCATCACCCAGCTTAAAGAAAGAGATCCTACATTTCCAAACGTGAAGAAAGGCATTCTTGTGCCTATGGTAACTCCTGGGTCACCTGCTGAACGAGCCGGGTTCCGTTCTGGTGATGTCGTTATTGAATTTGATGGAAACGCTGTTGCAAGCATCAAGGAGATCATTGAAATAATGGGGGACCGAGTCGGGGTGCCCATCGGGGTAGTCGTCATACGAGCGAGAGACGATACAGTGACCTTAACAGTGATCCCAGAGGAGTCCAATTCAGATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04210 ko04214 ko04215 ko05012 map04210 map04214 map04215 map05012
Pfam Domains
Protein Families
PDZ_2 Trypsin_2

Protein Analysis

417

Amino Acids

43.71

Weight (kDa)

6.25

Isoelectric Point (pI)

36.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1066
Acc16I TGCGCA 1 cut(s) 766
Acc36I ACCTGC 1 cut(s) 1066
Acc65I GGTACC 1 cut(s) 521
AccB1I GGYRCC 2 cut(s) 521, 1165
AccB7I CCANNNNNTGG 1 cut(s) 275
AccII CGCG 2 cut(s) 138, 613
AciI CCGC 5 cut(s) 29, 42, 51, 54, 739
AclWI GGATC 3 cut(s) 374, 992, 1216
AcsI RAATTY 4 cut(s) 19, 571, 781, 1100
AcuI CTGAAG 2 cut(s) 273, 650
AfaI GTAC 2 cut(s) 418, 523
AfiI CCNNNNNNNGG 4 cut(s) 60, 275, 941, 1162
AgsI TTSAA 5 cut(s) 204, 623, 910, 1100, 1142
AhlI ACTAGT 1 cut(s) 197
AjnI CCWGG 2 cut(s) 625, 1048
AluBI AGCT 6 cut(s) 151, 467, 601, 644, 683, 985
AluI AGCT 6 cut(s) 151, 467, 601, 644, 683, 985
Alw21I GWGCWC 2 cut(s) 716, 906
Alw26I GTCTC 1 cut(s) 1191
AlwI GGATC 3 cut(s) 374, 992, 1216
Ama87I CYCGRG 1 cut(s) 775
AoxI GGCC 4 cut(s) 258, 457, 791, 932
ApeKI GCWGC 6 cut(s) 56, 87, 148, 308, 311, 848
ApoI RAATTY 4 cut(s) 19, 571, 781, 1100
Asp700I GAANNNNTTC 1 cut(s) 199
Asp718I GGTACC 1 cut(s) 521
AspLEI GCGC 3 cut(s) 138, 434, 767
AspS9I GGNCC 4 cut(s) 259, 323, 791, 1153
AsuC2I CCSGG 4 cut(s) 375, 776, 777, 1072
AsuHPI GGTGA 6 cut(s) 484, 649, 880, 970, 1047, 1097
AvaI CYCGRG 1 cut(s) 775
AvaII GGWCC 2 cut(s) 323, 1153
BaeGI GKGCMC 1 cut(s) 1170
BaeI ACNNNNGTAYC 2 cut(s) 513, 546
BanI GGYRCC 2 cut(s) 521, 1165
Bbv12I GWGCWC 2 cut(s) 716, 906
BbvI GCAGC 6 cut(s) 43, 99, 160, 295, 298, 835
BccI CCATC 6 cut(s) 217, 497, 848, 946, 1100, 1178
BceAI ACGGC 2 cut(s) 100, 123
BciT130I CCWGG 2 cut(s) 627, 1050
BcnI CCSGG 4 cut(s) 375, 776, 777, 1072
BcoDI GTCTC 1 cut(s) 1191
BcuI ACTAGT 1 cut(s) 197
BfaI CTAG 2 cut(s) 198, 602
BfmI CTRYAG 3 cut(s) 57, 441, 558
BfuAI ACCTGC 1 cut(s) 1066
BisI GCNGC 9 cut(s) 42, 51, 54, 57, 88, 149, 309, 312, 849
BlsI GCNGC 9 cut(s) 43, 52, 55, 58, 89, 150, 310, 313, 850
Bme1390I CCNGG 6 cut(s) 375, 627, 776, 777, 1050, 1072
Bme18I GGWCC 2 cut(s) 323, 1153
BmeT110I CYCGRG 1 cut(s) 775
BmgT120I GGNCC 4 cut(s) 259, 323, 791, 1153
BmiI GGNNCC 7 cut(s) 177, 261, 523, 792, 1076, 1154, 1167
BmrFI CCNGG 6 cut(s) 375, 627, 776, 777, 1050, 1072
BmsI GCATC 2 cut(s) 119, 1134
BpmI CTGGAG 1 cut(s) 333
Bpu10I CCTNAGC 1 cut(s) 264
BpuMI CCSGG 4 cut(s) 375, 776, 777, 1072
BsaJI CCNNGG 6 cut(s) 155, 525, 626, 775, 935, 1049
BsaWI WCCGGW 1 cut(s) 33
Bsc4I CCNNNNNNNGG 4 cut(s) 60, 275, 941, 1162
Bse3DI GCAATG 2 cut(s) 45, 131
BseBI CCWGG 2 cut(s) 627, 1050
BseDI CCNNGG 6 cut(s) 155, 525, 626, 775, 935, 1049
BseGI GGATG 1 cut(s) 284
BseLI CCNNNNNNNGG 4 cut(s) 60, 275, 941, 1162
BseMI GCAATG 2 cut(s) 45, 131
BseMII CTCAG 2 cut(s) 452, 957
BseRI GAGGAG 1 cut(s) 1244
BseSI GKGCMC 1 cut(s) 1170
BseXI GCAGC 6 cut(s) 43, 99, 160, 295, 298, 835
BseYI CCCAGC 1 cut(s) 981
BsgI GTGCAG 1 cut(s) 111
Bsh1236I CGCG 2 cut(s) 138, 613
BshFI GGCC 4 cut(s) 260, 459, 793, 934
BshNI GGYRCC 2 cut(s) 521, 1165
BsiHKAI GWGCWC 2 cut(s) 716, 906
BsiHKCI CYCGRG 1 cut(s) 775
BsiSI CCGG 4 cut(s) 34, 374, 776, 1071
BslFI GGGAC 2 cut(s) 409, 1166
BslI CCNNNNNNNGG 4 cut(s) 60, 275, 941, 1162
BsmAI GTCTC 1 cut(s) 1191
BsmBI CGTCTC 1 cut(s) 1191
BsmFI GGGAC 2 cut(s) 409, 1166
BsmI GAATGC 1 cut(s) 1026
BsnI GGCC 4 cut(s) 260, 459, 793, 934
BsoBI CYCGRG 1 cut(s) 775
Bsp1286I GDGCHC 3 cut(s) 716, 906, 1170
Bsp143I GATC 5 cut(s) 366, 958, 997, 1134, 1221
Bsp19I CCATGG 1 cut(s) 155
BspACI CCGC 5 cut(s) 29, 42, 51, 54, 739
BspANI GGCC 4 cut(s) 260, 459, 793, 934
BspCNI CTCAG 2 cut(s) 453, 958
BspFNI CGCG 2 cut(s) 138, 613
BspLI GGNNCC 7 cut(s) 177, 261, 523, 792, 1076, 1154, 1167
BspMAI CTGCAG 1 cut(s) 61
BspMI ACCTGC 1 cut(s) 1066
BspPI GGATC 3 cut(s) 374, 992, 1216
BspT107I GGYRCC 2 cut(s) 521, 1165
BsrDI GCAATG 2 cut(s) 45, 131
BssECI CCNNGG 6 cut(s) 155, 525, 626, 775, 935, 1049
BssMI GATC 5 cut(s) 366, 958, 997, 1134, 1221
BssT1I CCWWGG 2 cut(s) 155, 935
Bst2UI CCWGG 2 cut(s) 627, 1050
Bst4CI ACNGT 7 cut(s) 183, 416, 442, 526, 676, 1207, 1219
Bst6I CTCTTC 1 cut(s) 249
BstAPI GCANNNNNTGC 1 cut(s) 308
BstC8I GCNNGC 1 cut(s) 1123
BstDEI CTNAG 3 cut(s) 264, 461, 966
BstDSI CCRYGG 2 cut(s) 155, 525
BstEII GGTNACC 1 cut(s) 1053
BstF5I GGATG 1 cut(s) 284
BstFNI CGCG 2 cut(s) 138, 613
BstHHI GCGC 3 cut(s) 138, 434, 767
BstKTI GATC 5 cut(s) 369, 961, 1000, 1137, 1224
BstMAI GTCTC 1 cut(s) 1191
BstMBI GATC 5 cut(s) 366, 958, 997, 1134, 1221
BstMWI GCNNNNNNNGC 8 cut(s) 47, 50, 56, 65, 157, 166, 308, 1067
BstNI CCWGG 2 cut(s) 627, 1050
BstNSI RCATGY 1 cut(s) 432
BstPI GGTNACC 1 cut(s) 1053
BstSCI CCNGG 6 cut(s) 373, 625, 774, 775, 1048, 1070
BstSFI CTRYAG 3 cut(s) 57, 441, 558
BstSLI GKGCMC 1 cut(s) 1170
BstUI CGCG 2 cut(s) 138, 613
BstV1I GCAGC 6 cut(s) 43, 99, 160, 295, 298, 835
BstX2I RGATCY 1 cut(s) 997
BstXI CCANNNNNNTGG 1 cut(s) 898
BstYI RGATCY 1 cut(s) 997
BsuRI GGCC 4 cut(s) 260, 459, 793, 934
BtgI CCRYGG 2 cut(s) 155, 525
BtsCI GGATG 1 cut(s) 284
BtsI GCAGTG 2 cut(s) 123, 584
BtsIMutI CAGTG 8 cut(s) 120, 123, 179, 584, 714, 963, 1212, 1224
BveI ACCTGC 1 cut(s) 1066
Cac8I GCNNGC 1 cut(s) 1123
CfoI GCGC 3 cut(s) 138, 434, 767
Cfr13I GGNCC 4 cut(s) 259, 323, 791, 1153
Cfr9I CCCGGG 1 cut(s) 775
Csp6I GTAC 2 cut(s) 417, 522
CspCI CAANNNNNGTGG 2 cut(s) 898, 933
CviAII CATG 5 cut(s) 156, 270, 429, 488, 898
CviQI GTAC 2 cut(s) 417, 522
DdeI CTNAG 3 cut(s) 264, 461, 966
DpnI GATC 5 cut(s) 368, 960, 999, 1136, 1223
DpnII GATC 5 cut(s) 366, 958, 997, 1134, 1221
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
Eco130I CCWWGG 2 cut(s) 155, 935
Eco47I GGWCC 2 cut(s) 323, 1153
Eco57I CTGAAG 2 cut(s) 273, 650
Eco88I CYCGRG 1 cut(s) 775
Eco91I GGTNACC 1 cut(s) 1053
EcoO109I RGGNCCY 2 cut(s) 259, 791
EcoO65I GGTNACC 1 cut(s) 1053
EcoRII CCWGG 2 cut(s) 625, 1048
EcoT14I CCWWGG 2 cut(s) 155, 935
ErhI CCWWGG 2 cut(s) 155, 935
Esp3I CGTCTC 1 cut(s) 1191
FaeI CATG 5 cut(s) 159, 273, 432, 491, 901
FalI AAGNNNNNCTT 2 cut(s) 1014, 1046
FaqI GGGAC 2 cut(s) 409, 1166
FatI CATG 5 cut(s) 155, 269, 428, 487, 897
FauNDI CATATG 1 cut(s) 361
Fnu4HI GCNGC 9 cut(s) 42, 51, 54, 57, 88, 149, 309, 312, 849
FokI GGATG 1 cut(s) 291
Fsp4HI GCNGC 9 cut(s) 42, 51, 54, 57, 88, 149, 309, 312, 849
FspBI CTAG 2 cut(s) 198, 602
FspI TGCGCA 1 cut(s) 766
GlaI GCGC 3 cut(s) 137, 433, 766
GluI GCNGC 9 cut(s) 42, 51, 54, 57, 88, 149, 309, 312, 849
GsaI CCCAGC 1 cut(s) 985
GsuI CTGGAG 1 cut(s) 333
HaeIII GGCC 4 cut(s) 260, 459, 793, 934
HapII CCGG 4 cut(s) 34, 374, 776, 1071
HhaI GCGC 3 cut(s) 138, 434, 767
Hin1II CATG 5 cut(s) 159, 273, 432, 491, 901
Hin6I GCGC 3 cut(s) 136, 432, 765
HinP1I GCGC 3 cut(s) 136, 432, 765
HincII GTYRAC 2 cut(s) 337, 703
HindII GTYRAC 2 cut(s) 337, 703
HinfI GANTC 5 cut(s) 140, 293, 881, 1158, 1232
HpaII CCGG 4 cut(s) 34, 374, 776, 1071
HphI GGTGA 6 cut(s) 484, 649, 880, 970, 1047, 1097
Hpy166II GTNNAC 4 cut(s) 337, 617, 656, 703
Hpy188I TCNGA 8 cut(s) 15, 82, 100, 217, 253, 550, 669, 1243
Hpy188III TCNNGA 3 cut(s) 276, 350, 910
Hpy8I GTNNAC 4 cut(s) 337, 617, 656, 703
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 5 cut(s) 220, 258, 310, 475, 880
HpyCH4III ACNGT 7 cut(s) 183, 416, 442, 526, 676, 1207, 1219
HpyCH4IV ACGT 1 cut(s) 1014
HpyCH4V TGCA 7 cut(s) 59, 128, 148, 359, 499, 536, 1121
HpyF10VI GCNNNNNNNGC 8 cut(s) 47, 50, 56, 65, 157, 166, 308, 1067
HpyF3I CTNAG 3 cut(s) 264, 461, 966
HpySE526I ACGT 1 cut(s) 1014
Hsp92II CATG 5 cut(s) 159, 273, 432, 491, 901
HspAI GCGC 3 cut(s) 136, 432, 765
KpnI GGTACC 1 cut(s) 525
Kzo9I GATC 5 cut(s) 366, 958, 997, 1134, 1221
LmnI GCTCC 3 cut(s) 181, 352, 901
Lsp1109I GCAGC 6 cut(s) 43, 99, 160, 295, 298, 835
LweI GCATC 2 cut(s) 119, 1134
MaeI CTAG 2 cut(s) 198, 602
MaeII ACGT 1 cut(s) 1014
MaeIII GTNAC 6 cut(s) 490, 676, 886, 1042, 1053, 1207
MalI GATC 5 cut(s) 368, 960, 999, 1136, 1223
MboI GATC 5 cut(s) 366, 958, 997, 1134, 1221
MboII GAAGA 2 cut(s) 266, 1030
MfeI CAATTG 1 cut(s) 876
MflI RGATCY 1 cut(s) 997
MhlI GDGCHC 3 cut(s) 716, 906, 1170
MluCI AATT 8 cut(s) 19, 204, 420, 571, 781, 876, 1100, 1237
MlyI GAGTC 2 cut(s) 1167, 1241
MmeI TCCRAC 2 cut(s) 105, 922
MnlI CCTC 7 cut(s) 250, 346, 403, 511, 915, 961, 1222
MroXI GAANNNNTTC 1 cut(s) 199
MseI TTAA 4 cut(s) 425, 828, 987, 1214
MslI CAYNNNNRTG 1 cut(s) 1031
MspA1I CMGCKG 2 cut(s) 56, 683
MspI CCGG 4 cut(s) 34, 374, 776, 1071
MspR9I CCNGG 6 cut(s) 375, 627, 776, 777, 1050, 1072
MunI CAATTG 1 cut(s) 876
Mva1269I GAATGC 1 cut(s) 1026
MvaI CCWGG 2 cut(s) 627, 1050
MvnI CGCG 2 cut(s) 138, 613
MwoI GCNNNNNNNGC 8 cut(s) 47, 50, 56, 65, 157, 166, 308, 1067
NciI CCSGG 4 cut(s) 375, 776, 777, 1072
NcoI CCATGG 1 cut(s) 155
NdeI CATATG 1 cut(s) 361
NdeII GATC 5 cut(s) 366, 958, 997, 1134, 1221
NlaIII CATG 5 cut(s) 159, 273, 432, 491, 901
NlaIV GGNNCC 7 cut(s) 177, 261, 523, 792, 1076, 1154, 1167
NmuCI GTSAC 5 cut(s) 490, 676, 886, 1053, 1207
NsbI TGCGCA 1 cut(s) 766
NspI RCATGY 1 cut(s) 432
PaqCI CACCTGC 1 cut(s) 1066
PcsI WCGNNNNNNNCGW 1 cut(s) 1179
PctI GAATGC 1 cut(s) 1026
PdmI GAANNNNTTC 1 cut(s) 199
PfeI GAWTC 3 cut(s) 140, 293, 881
PflFI GACNNNGTC 1 cut(s) 1157
PflMI CCANNNNNTGG 1 cut(s) 275
PfoI TCCNGGA 1 cut(s) 373
PkrI GCNGC 9 cut(s) 43, 52, 55, 58, 89, 150, 310, 313, 850
PleI GAGTC 2 cut(s) 1166, 1240
PpsI GAGTC 2 cut(s) 1166, 1240
Psp6I CCWGG 2 cut(s) 625, 1048
PspEI GGTNACC 1 cut(s) 1053
PspFI CCCAGC 1 cut(s) 981
PspGI CCWGG 2 cut(s) 625, 1048
PspN4I GGNNCC 7 cut(s) 177, 261, 523, 792, 1076, 1154, 1167
PspPI GGNCC 4 cut(s) 259, 323, 791, 1153
PstI CTGCAG 1 cut(s) 61
PsuI RGATCY 1 cut(s) 997
PsyI GACNNNGTC 1 cut(s) 1157
PvuII CAGCTG 1 cut(s) 683
RsaI GTAC 2 cut(s) 418, 523
RsaNI GTAC 2 cut(s) 417, 522
RseI CAYNNNNRTG 1 cut(s) 1031
SaqAI TTAA 4 cut(s) 425, 828, 987, 1214
SatI GCNGC 9 cut(s) 42, 51, 54, 57, 88, 149, 309, 312, 849
Sau3AI GATC 5 cut(s) 366, 958, 997, 1134, 1221
Sau96I GGNCC 4 cut(s) 259, 323, 791, 1153
SchI GAGTC 2 cut(s) 1167, 1241
ScrFI CCNGG 6 cut(s) 375, 627, 776, 777, 1050, 1072
SduI GDGCHC 3 cut(s) 716, 906, 1170
SfaNI GCATC 2 cut(s) 119, 1134
SfcI CTRYAG 3 cut(s) 57, 441, 558
SinI GGWCC 2 cut(s) 323, 1153
SmaI CCCGGG 1 cut(s) 777
SmiMI CAYNNNNRTG 1 cut(s) 1031
SpeI ACTAGT 1 cut(s) 197
Sse9I AATT 8 cut(s) 19, 204, 420, 571, 781, 876, 1100, 1237
SsiI CCGC 5 cut(s) 29, 42, 51, 54, 739
SspI AATATT 2 cut(s) 805, 832
SspMI CTAG 2 cut(s) 198, 602
StyD4I CCNGG 6 cut(s) 373, 625, 774, 775, 1048, 1070
StyI CCWWGG 2 cut(s) 155, 935
TaaI ACNGT 7 cut(s) 183, 416, 442, 526, 676, 1207, 1219
TaiI ACGT 1 cut(s) 1017
TaqII GACCGA 2 cut(s) 720, 1170
TasI AATT 8 cut(s) 19, 204, 420, 571, 781, 876, 1100, 1237
TauI GCSGC 3 cut(s) 44, 53, 56
TfiI GAWTC 3 cut(s) 140, 293, 881
Tru1I TTAA 4 cut(s) 425, 828, 987, 1214
Tru9I TTAA 4 cut(s) 425, 828, 987, 1214
TscAI CASTG 8 cut(s) 120, 130, 186, 591, 721, 970, 1212, 1224
TseFI GTSAC 5 cut(s) 490, 676, 886, 1053, 1207
TseI GCWGC 6 cut(s) 56, 87, 148, 308, 311, 848
Tsp45I GTSAC 5 cut(s) 490, 676, 886, 1053, 1207
TspDTI ATGAA 4 cut(s) 17, 459, 476, 851
TspGWI ACGGA 2 cut(s) 825, 1067
TspMI CCCGGG 1 cut(s) 775
TspRI CASTG 8 cut(s) 120, 130, 186, 591, 721, 970, 1212, 1224
Tth111I GACNNNGTC 1 cut(s) 1157
Van91I CCANNNNNTGG 1 cut(s) 275
VpaK11BI GGWCC 2 cut(s) 323, 1153
XapI RAATTY 4 cut(s) 19, 571, 781, 1100
XceI RCATGY 1 cut(s) 432
XmaI CCCGGG 1 cut(s) 775
XmnI GAANNNNTTC 1 cut(s) 199
XspI CTAG 2 cut(s) 198, 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.