FvH4_6g06420

protease Do-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005022
Physical Location & Seq
Reverse (-)
3741571 .. 3745106
3536 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g06420.t1

Sequence Viewer

Length: 1272 bp
ATGAGTCATTTTCTGAGGAATGTTTTGATTGCAAACCGGAACCGAAATCGGATTCTGGCCATTGCTGCCGTTGGTTCGGCTTTGTTATATGCCAAAGGGAACGAGAGTTTCAATTCTTCATTCTCGGTGTCGATTCCTGCACCATGGCGTGAATCGCTGTTGCTGCCAAGGCAAAACTGGCCATTCGGAGTTGTTCCATTGTTTTCTGTTACAAATGGTTCGGCTCCTTCTCCGGACATAGGGAAGGATGTGTCTGGTTTCTCTGTGGCGGGAGAGAGCCCAAAGCCATGCTGTAGTGGGTGTTTGGGCAAAGATACAATTGCAAAGGCCGCTGCAAAAGTCGGCCCTGCTGTTGTCAATATATCTCTTCAACAGGGTATGTATGGTGTTGGTGTTGGGAAGGGTATAGGCTCTGGAACTATAATTGATGAGGATGGCACGATCCTAACGTGTGCGCATGCTGTGGTTGATTTTCATGGGCTGAGAGCTTCATCAAAGGGGAAGGTTGGTGTAACTTTGCAAGATGGTCGGACATTTGAGGGTACTGTGGTGAATGCGGATCTCCAGTCTGATGTTGCTATAGTAAAGATCAATTCTAAAACTCCCCTTCCATCAGCGAAATTGGGCACCTCAAGTAGACTCCAACCTGGGGATTGGGTGATAGCAGTTGGTTGTCCACTTTCGCTTCAGAATACTGTCACATCTGGTATTGTAAGTTGTGTCGACCGCAAAAGTAGTGATTTGGGGCTTGGTGGACTGCGGAGGGAGTACTTACAGACCGATTGCGCTATTAACCCGGGGAATTCTGGGGGACCTCTAGTAAATATGGATGGAGAAGTTATTGGTGTTAATATCATGAAAGTACTTGCTGCTGATGGATTGAGTTTTGCTGTACCGATTGACTCAATTGCCAAAATAATGGAGCACTTTAAGAAAAATGGGAGGGTTGTTCGGCCTTGGCTTGGACTGAAGATGATTGACCTAAACGATATGATAATTTCTCAGCTCAAAGAAAGAGATAGTTCATTCCCAAGTATAAAAAGAGGGATTCTCGTACCTATGGTAACTCCTGGGTCACCTGCTGAGCGTGCTGGGTTTCGTCCTGGTGATGTTGTTGTCGAATTTGATGGAAAGCCTGTTGCAAGCATCATAGAGGTCACTGAAATAATGGGAGATAGAGTTGGAGTACCATTGAAGGTTGTCGTGAAGAGGGCGAAAGATGAGGTGGTGACTTTGACAGTAATAGCAGAGGAATCCCATACAGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04210 ko04214 ko04215 ko05012 map04210 map04214 map04215 map05012
Pfam Domains
Protein Families
PDZ_2 Trypsin_2

Protein Analysis

424

Amino Acids

44.53

Weight (kDa)

8.6

Isoelectric Point (pI)

36.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trypsin PF00089 115 - 305 3.3e-18 Trypsin
Trypsin_2 PF13365 137 - 283 1.6e-33 Trypsin-like peptidase domain
PDZ_2 PF13180 321 - 414 3.6e-10 PDZ domain
PDZ_6 PF17820 355 - 402 3.7e-11 PDZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1087
Acc16I TGCGCA 1 cut(s) 456
Acc36I ACCTGC 1 cut(s) 1087
AccB1I GGYRCC 1 cut(s) 626
AccI GTMKAC 2 cut(s) 637, 723
AccIII TCCGGA 1 cut(s) 232
AciI CCGC 5 cut(s) 269, 330, 557, 727, 760
AclWI GGATC 2 cut(s) 436, 567
AcoI YGGCCR 2 cut(s) 57, 179
AcsI RAATTY 2 cut(s) 802, 1121
AcuI CTGAAG 2 cut(s) 671, 989
AfaI GTAC 6 cut(s) 544, 770, 864, 894, 1056, 1188
AfiI CCNNNNNNNGG 3 cut(s) 239, 649, 962
AflIII ACRYGT 1 cut(s) 449
AgsI TTSAA 3 cut(s) 112, 371, 1195
AjnI CCWGG 3 cut(s) 646, 1069, 1102
AluBI AGCT 2 cut(s) 488, 1006
AluI AGCT 2 cut(s) 488, 1006
Alw21I GWGCWC 1 cut(s) 927
AlwI GGATC 2 cut(s) 436, 567
Ama87I CYCGRG 1 cut(s) 796
Aor13HI TCCGGA 1 cut(s) 232
AoxI GGCC 5 cut(s) 57, 179, 327, 343, 953
ApeKI GCWGC 4 cut(s) 65, 163, 332, 869
ApoI RAATTY 2 cut(s) 802, 1121
AspLEI GCGC 2 cut(s) 457, 788
AspS9I GGNCC 2 cut(s) 344, 812
AsuC2I CCSGG 2 cut(s) 797, 798
AsuHPI GGTGA 5 cut(s) 562, 670, 1068, 1118, 1240
AvaI CYCGRG 1 cut(s) 796
AvaII GGWCC 1 cut(s) 812
BaeGI GKGCMC 1 cut(s) 629
BaeI ACNNNNGTAYC 2 cut(s) 534, 567
BalI TGGCCA 2 cut(s) 59, 181
BanI GGYRCC 1 cut(s) 626
BanII GRGCYC 1 cut(s) 281
Bbv12I GWGCWC 1 cut(s) 927
BbvI GCAGC 4 cut(s) 52, 150, 319, 856
BccI CCATC 6 cut(s) 428, 518, 619, 824, 869, 1121
BceAI ACGGC 1 cut(s) 53
BcgI CGANNNNNNTGC 2 cut(s) 509, 543
BciT130I CCWGG 3 cut(s) 648, 1071, 1104
BcnI CCSGG 2 cut(s) 797, 798
BfaI CTAG 1 cut(s) 818
BfmI CTRYAG 2 cut(s) 292, 579
BfuAI ACCTGC 1 cut(s) 1087
BisI GCNGC 5 cut(s) 66, 164, 330, 333, 870
BlpI GCTNAGC 1 cut(s) 1083
BlsI GCNGC 5 cut(s) 67, 165, 331, 334, 871
BmcAI AGTACT 2 cut(s) 770, 864
Bme1390I CCNGG 5 cut(s) 648, 797, 798, 1071, 1104
Bme18I GGWCC 1 cut(s) 812
BmeT110I CYCGRG 1 cut(s) 796
BmgT120I GGNCC 2 cut(s) 344, 812
BmiI GGNNCC 4 cut(s) 41, 225, 628, 813
BmrFI CCNGG 5 cut(s) 648, 797, 798, 1071, 1104
BmsI GCATC 1 cut(s) 1155
BplI GAGNNNNNCTC 2 cut(s) 1035, 1067
BpmI CTGGAG 1 cut(s) 548
Bpu1102I GCTNAGC 1 cut(s) 1083
BpuEI CTTGAG 1 cut(s) 616
BpuMI CCSGG 2 cut(s) 797, 798
BsaJI CCNNGG 7 cut(s) 143, 167, 647, 796, 797, 956, 1070
BsaWI WCCGGW 2 cut(s) 36, 232
BsaXI ACNNNNNCTCC 2 cut(s) 1164, 1194
Bsc4I CCNNNNNNNGG 3 cut(s) 239, 649, 962
Bse1I ACTGG 2 cut(s) 182, 565
Bse3DI GCAATG 1 cut(s) 60
BseAI TCCGGA 1 cut(s) 232
BseBI CCWGG 3 cut(s) 648, 1071, 1104
BseDI CCNNGG 7 cut(s) 143, 167, 647, 796, 797, 956, 1070
BseGI GGATG 3 cut(s) 253, 439, 835
BseLI CCNNNNNNNGG 3 cut(s) 239, 649, 962
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 4 cut(s) 5, 473, 1016, 1074
BseNI ACTGG 2 cut(s) 182, 565
BseSI GKGCMC 1 cut(s) 629
BseXI GCAGC 4 cut(s) 52, 150, 319, 856
BseYI CCCAGC 1 cut(s) 1091
BsgI GTGCAG 1 cut(s) 123
Bsh1285I CGRYCG 1 cut(s) 727
BshFI GGCC 5 cut(s) 59, 181, 329, 345, 955
BshNI GGYRCC 1 cut(s) 626
BsiEI CGRYCG 1 cut(s) 727
BsiHKAI GWGCWC 1 cut(s) 927
BsiHKCI CYCGRG 1 cut(s) 796
BsiSI CCGG 3 cut(s) 37, 233, 797
BslFI GGGAC 1 cut(s) 825
BslI CCNNNNNNNGG 3 cut(s) 239, 649, 962
BsmFI GGGAC 1 cut(s) 825
BsmI GAATGC 1 cut(s) 559
BsnI GGCC 5 cut(s) 59, 181, 329, 345, 955
BsoBI CYCGRG 1 cut(s) 796
Bsp1286I GDGCHC 3 cut(s) 281, 629, 927
Bsp13I TCCGGA 1 cut(s) 232
Bsp143I GATC 3 cut(s) 441, 559, 588
Bsp1720I GCTNAGC 1 cut(s) 1083
Bsp19I CCATGG 1 cut(s) 143
BspACI CCGC 5 cut(s) 269, 330, 557, 727, 760
BspANI GGCC 5 cut(s) 59, 181, 329, 345, 955
BspCNI CTCAG 4 cut(s) 6, 474, 1015, 1075
BspEI TCCGGA 1 cut(s) 232
BspHI TCATGA 1 cut(s) 855
BspLI GGNNCC 4 cut(s) 41, 225, 628, 813
BspMI ACCTGC 1 cut(s) 1087
BspPI GGATC 2 cut(s) 436, 567
BspT107I GGYRCC 1 cut(s) 626
BsrDI GCAATG 1 cut(s) 60
BsrI ACTGG 2 cut(s) 182, 565
BssECI CCNNGG 7 cut(s) 143, 167, 647, 796, 797, 956, 1070
BssMI GATC 3 cut(s) 441, 559, 588
BssT1I CCWWGG 3 cut(s) 143, 167, 956
Bst2UI CCWGG 3 cut(s) 648, 1071, 1104
Bst4CI ACNGT 3 cut(s) 547, 697, 1240
Bst6I CTCTTC 2 cut(s) 372, 1202
BstC8I GCNNGC 3 cut(s) 459, 1089, 1144
BstDEI CTNAG 4 cut(s) 14, 482, 1002, 1083
BstDSI CCRYGG 1 cut(s) 143
BstEII GGTNACC 1 cut(s) 1074
BstF5I GGATG 3 cut(s) 253, 439, 835
BstHHI GCGC 2 cut(s) 457, 788
BstKTI GATC 3 cut(s) 444, 562, 591
BstMBI GATC 3 cut(s) 441, 559, 588
BstMCI CGRYCG 1 cut(s) 727
BstMWI GCNNNNNNNGC 7 cut(s) 65, 154, 163, 169, 178, 329, 1088
BstNI CCWGG 3 cut(s) 648, 1071, 1104
BstNSI RCATGY 1 cut(s) 461
BstPI GGTNACC 1 cut(s) 1074
BstSCI CCNGG 5 cut(s) 646, 795, 796, 1069, 1102
BstSFI CTRYAG 2 cut(s) 292, 579
BstSLI GKGCMC 1 cut(s) 629
BstV1I GCAGC 4 cut(s) 52, 150, 319, 856
BstX2I RGATCY 1 cut(s) 559
BstXI CCANNNNNNTGG 1 cut(s) 919
BstYI RGATCY 1 cut(s) 559
BsuRI GGCC 5 cut(s) 59, 181, 329, 345, 955
BtgI CCRYGG 1 cut(s) 143
BtsCI GGATG 3 cut(s) 253, 439, 835
BtsIMutI CAGTG 1 cut(s) 1158
BveI ACCTGC 1 cut(s) 1087
Cac8I GCNNGC 3 cut(s) 459, 1089, 1144
CciI TCATGA 1 cut(s) 855
CfoI GCGC 2 cut(s) 457, 788
Cfr13I GGNCC 2 cut(s) 344, 812
Cfr9I CCCGGG 1 cut(s) 796
Csp6I GTAC 6 cut(s) 543, 769, 863, 893, 1055, 1187
CviAII CATG 5 cut(s) 144, 288, 458, 476, 856
CviQI GTAC 6 cut(s) 543, 769, 863, 893, 1055, 1187
DdeI CTNAG 4 cut(s) 14, 482, 1002, 1083
DpnI GATC 3 cut(s) 443, 561, 590
DpnII GATC 3 cut(s) 441, 559, 588
EaeI YGGCCR 2 cut(s) 57, 179
Eam1104I CTCTTC 2 cut(s) 372, 1202
EarI CTCTTC 2 cut(s) 372, 1202
Eco130I CCWWGG 3 cut(s) 143, 167, 956
Eco24I GRGCYC 1 cut(s) 281
Eco47I GGWCC 1 cut(s) 812
Eco57I CTGAAG 2 cut(s) 671, 989
Eco88I CYCGRG 1 cut(s) 796
Eco91I GGTNACC 1 cut(s) 1074
EcoO109I RGGNCCY 1 cut(s) 812
EcoO65I GGTNACC 1 cut(s) 1074
EcoRI GAATTC 1 cut(s) 802
EcoRII CCWGG 3 cut(s) 646, 1069, 1102
EcoT14I CCWWGG 3 cut(s) 143, 167, 956
EcoT38I GRGCYC 1 cut(s) 281
ErhI CCWWGG 3 cut(s) 143, 167, 956
FaeI CATG 5 cut(s) 147, 291, 461, 479, 859
FaqI GGGAC 1 cut(s) 825
FatI CATG 5 cut(s) 143, 287, 457, 475, 855
FauI CCCGC 1 cut(s) 262
FblI GTMKAC 2 cut(s) 637, 723
Fnu4HI GCNGC 5 cut(s) 66, 164, 330, 333, 870
FokI GGATG 3 cut(s) 260, 446, 842
FriOI GRGCYC 1 cut(s) 281
Fsp4HI GCNGC 5 cut(s) 66, 164, 330, 333, 870
FspAI RTGCGCAY 1 cut(s) 456
FspBI CTAG 1 cut(s) 818
FspI TGCGCA 1 cut(s) 456
GlaI GCGC 2 cut(s) 456, 787
GluI GCNGC 5 cut(s) 66, 164, 330, 333, 870
GsaI CCCAGC 1 cut(s) 1095
GsuI CTGGAG 1 cut(s) 548
HaeIII GGCC 5 cut(s) 59, 181, 329, 345, 955
HapII CCGG 3 cut(s) 37, 233, 797
HhaI GCGC 2 cut(s) 457, 788
Hin1II CATG 5 cut(s) 147, 291, 461, 479, 859
Hin6I GCGC 2 cut(s) 455, 786
HinP1I GCGC 2 cut(s) 455, 786
HincII GTYRAC 1 cut(s) 724
HindII GTYRAC 1 cut(s) 724
HinfI GANTC 8 cut(s) 4, 52, 133, 152, 639, 902, 1048, 1253
HpaII CCGG 3 cut(s) 37, 233, 797
HphI GGTGA 5 cut(s) 562, 670, 1068, 1118, 1240
Hpy166II GTNNAC 4 cut(s) 638, 677, 724, 755
Hpy188I TCNGA 6 cut(s) 15, 51, 188, 531, 571, 690
Hpy188III TCNNGA 4 cut(s) 233, 414, 856, 1204
Hpy8I GTNNAC 4 cut(s) 638, 677, 724, 755
HpyAV CCTTC 6 cut(s) 237, 238, 394, 496, 617, 1189
HpyCH4III ACNGT 3 cut(s) 547, 697, 1240
HpyCH4IV ACGT 1 cut(s) 449
HpyCH4V TGCA 6 cut(s) 32, 140, 323, 335, 520, 1142
HpyF10VI GCNNNNNNNGC 7 cut(s) 65, 154, 163, 169, 178, 329, 1088
HpyF3I CTNAG 4 cut(s) 14, 482, 1002, 1083
HpySE526I ACGT 1 cut(s) 449
Hsp92II CATG 5 cut(s) 147, 291, 461, 479, 859
HspAI GCGC 2 cut(s) 455, 786
Kpn2I TCCGGA 1 cut(s) 232
Kzo9I GATC 3 cut(s) 441, 559, 588
LmnI GCTCC 2 cut(s) 229, 922
Lsp1109I GCAGC 4 cut(s) 52, 150, 319, 856
LweI GCATC 1 cut(s) 1155
MaeI CTAG 1 cut(s) 818
MaeII ACGT 1 cut(s) 449
MaeIII GTNAC 7 cut(s) 208, 511, 697, 1063, 1074, 1156, 1228
MalI GATC 3 cut(s) 443, 561, 590
MboI GATC 3 cut(s) 441, 559, 588
MboII GAAGA 4 cut(s) 108, 359, 982, 1219
MfeI CAATTG 2 cut(s) 318, 906
MflI RGATCY 1 cut(s) 559
MhlI GDGCHC 3 cut(s) 281, 629, 927
MlsI TGGCCA 2 cut(s) 59, 181
MluCI AATT 9 cut(s) 112, 318, 423, 592, 620, 802, 906, 996, 1121
MluNI TGGCCA 2 cut(s) 59, 181
MlyI GAGTC 3 cut(s) 13, 633, 896
MmeI TCCRAC 3 cut(s) 509, 667, 1162
Mox20I TGGCCA 2 cut(s) 59, 181
MroI TCCGGA 1 cut(s) 232
MscI TGGCCA 2 cut(s) 59, 181
MseI TTAA 3 cut(s) 792, 849, 930
Msp20I TGGCCA 2 cut(s) 59, 181
MspA1I CMGCKG 1 cut(s) 332
MspI CCGG 3 cut(s) 37, 233, 797
MspR9I CCNGG 5 cut(s) 648, 797, 798, 1071, 1104
MunI CAATTG 2 cut(s) 318, 906
Mva1269I GAATGC 1 cut(s) 559
MvaI CCWGG 3 cut(s) 648, 1071, 1104
MwoI GCNNNNNNNGC 7 cut(s) 65, 154, 163, 169, 178, 329, 1088
NciI CCSGG 2 cut(s) 797, 798
NcoI CCATGG 1 cut(s) 143
NdeII GATC 3 cut(s) 441, 559, 588
NlaIII CATG 5 cut(s) 147, 291, 461, 479, 859
NlaIV GGNNCC 4 cut(s) 41, 225, 628, 813
NmuCI GTSAC 4 cut(s) 697, 1074, 1156, 1228
NsbI TGCGCA 1 cut(s) 456
NspI RCATGY 1 cut(s) 461
PaeI GCATGC 1 cut(s) 461
PagI TCATGA 1 cut(s) 855
PaqCI CACCTGC 1 cut(s) 1087
PcsI WCGNNNNNNNCGW 1 cut(s) 446
PctI GAATGC 1 cut(s) 559
PfeI GAWTC 5 cut(s) 52, 133, 152, 1048, 1253
PkrI GCNGC 5 cut(s) 67, 165, 331, 334, 871
PleI GAGTC 3 cut(s) 12, 633, 896
PpsI GAGTC 3 cut(s) 12, 633, 896
PpuMI RGGWCCY 1 cut(s) 812
Psp5II RGGWCCY 1 cut(s) 812
Psp6I CCWGG 3 cut(s) 646, 1069, 1102
PspEI GGTNACC 1 cut(s) 1074
PspFI CCCAGC 1 cut(s) 1091
PspGI CCWGG 3 cut(s) 646, 1069, 1102
PspN4I GGNNCC 4 cut(s) 41, 225, 628, 813
PspPI GGNCC 2 cut(s) 344, 812
PspPPI RGGWCCY 1 cut(s) 812
PsuI RGATCY 1 cut(s) 559
RsaI GTAC 6 cut(s) 544, 770, 864, 894, 1056, 1188
RsaNI GTAC 6 cut(s) 543, 769, 863, 893, 1055, 1187
SalI GTCGAC 1 cut(s) 722
SaqAI TTAA 3 cut(s) 792, 849, 930
SatI GCNGC 5 cut(s) 66, 164, 330, 333, 870
Sau3AI GATC 3 cut(s) 441, 559, 588
Sau96I GGNCC 2 cut(s) 344, 812
ScaI AGTACT 2 cut(s) 770, 864
SchI GAGTC 3 cut(s) 13, 633, 896
ScrFI CCNGG 5 cut(s) 648, 797, 798, 1071, 1104
SduI GDGCHC 3 cut(s) 281, 629, 927
SfaNI GCATC 1 cut(s) 1155
SfcI CTRYAG 2 cut(s) 292, 579
SinI GGWCC 1 cut(s) 812
SmaI CCCGGG 1 cut(s) 798
SmlI CTYRAG 1 cut(s) 631
SmoI CTYRAG 1 cut(s) 631
SphI GCATGC 1 cut(s) 461
Sse9I AATT 9 cut(s) 112, 318, 423, 592, 620, 802, 906, 996, 1121
SsiI CCGC 5 cut(s) 269, 330, 557, 727, 760
SspMI CTAG 1 cut(s) 818
StyD4I CCNGG 5 cut(s) 646, 795, 796, 1069, 1102
StyI CCWWGG 3 cut(s) 143, 167, 956
TaaI ACNGT 3 cut(s) 547, 697, 1240
TaiI ACGT 1 cut(s) 452
TaqI TCGA 3 cut(s) 131, 723, 1119
TaqII GACCGA 1 cut(s) 794
TasI AATT 9 cut(s) 112, 318, 423, 592, 620, 802, 906, 996, 1121
TatI WGTACW 2 cut(s) 768, 862
TauI GCSGC 1 cut(s) 332
TfiI GAWTC 5 cut(s) 52, 133, 152, 1048, 1253
Tru1I TTAA 3 cut(s) 792, 849, 930
Tru9I TTAA 3 cut(s) 792, 849, 930
TscAI CASTG 1 cut(s) 1165
TseFI GTSAC 4 cut(s) 697, 1074, 1156, 1228
TseI GCWGC 4 cut(s) 65, 163, 332, 869
Tsp45I GTSAC 4 cut(s) 697, 1074, 1156, 1228
TspDTI ATGAA 5 cut(s) 108, 464, 480, 872, 1014
TspMI CCCGGG 1 cut(s) 796
TspRI CASTG 1 cut(s) 1165
VpaK11BI GGWCC 1 cut(s) 812
XapI RAATTY 2 cut(s) 802, 1121
XceI RCATGY 1 cut(s) 461
XcmI CCANNNNNNNNNTGG 1 cut(s) 174
XmaI CCCGGG 1 cut(s) 796
XmiI GTMKAC 2 cut(s) 637, 723
XspI CTAG 1 cut(s) 818
ZrmI AGTACT 2 cut(s) 770, 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.