AT5G37820

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006453
Physical Location & Seq
Forward (+)
15050002 .. 15051599
1598 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G37820.2

Sequence Viewer

Length: 852 bp
ATGACTTCTCATGGTGAAGAGATCGAAGACGAACAAATATCAAGAATTGAGAAAGGCAATTGCAAAGATAGCCAAGGAGGAATGGAGACGGCCATATGTAGTTCTCCTAGCATTGTTTGCCTTACACAAAAGTTGATAGCAGAGATGATTGGAACCTATTTCATAATATTCTCTGGATGTGGAGTAGTGGTAGTGAATGTCTTGTACGGAGGAACAATAACATTTCCAGGGATCTGTGTGACTTGGGGTCTCATTGTTATGGTCATGATTTACTCTACTGGTCACATTTCCGGTGCACATTTTAACCCTGCCGTCACTGTCACGTTCGCTGTTTTCCGGCGGTTTCCTTGGTATCAGGTACCATTGTATATAGGTGCACAACTTACGGGATCATTACTAGCGAGTTTAACATTGAGACTAATGTTCAATGTGACACCCAAAGCTTTCTTTGGAACAACTCCAACTGATTCATCAGGACAAGCACTAGTAGCAGAGATCATTATCTCATTCCTCCTCATGTTCGTTATCTCCGGCGTTGCCACTGATAGTCGTGCGACTGGAGAATTAGCTGGAATCGCGGTGGGGATGACTATAATATTGAACGTCTTTGTAGCCGGACCTATTTCGGGAGCATCAATGAATCCAGCGAGAAGTCTTGGACCAGCAATAGTGATGGGAAGATATAAAGGAATTTGGGTTTACATTGTTGGTCCCTTTGTCGGAATTTTTGCCGGAGGTTTTGTTTACAACTTCATGAGATTTACGGATAAGCCACTACGAGAACTCACAAAAAGTGCTTCTTTCCTCCGTAGTGTTGCCCAAAAAGACAATGCTTCTAAATCAGATGGCTAA

Protein Analysis

283

Amino Acids

30.28

Weight (kDa)

8.26

Isoelectric Point (pI)

26.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 77 - 285 1e-58 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 358
AccB1I GGYRCC 1 cut(s) 358
AccII CGCG 1 cut(s) 578
AciI CCGC 2 cut(s) 340, 578
AclWI GGATC 2 cut(s) 239, 397
AcoI YGGCCR 1 cut(s) 90
AcsI RAATTY 2 cut(s) 690, 723
AfaI GTAC 2 cut(s) 206, 360
AfiI CCNNNNNNNGG 2 cut(s) 626, 719
AgsI TTSAA 2 cut(s) 427, 601
AhdI GACNNNNNGTC 1 cut(s) 246
AhlI ACTAGT 1 cut(s) 484
AjnI CCWGG 1 cut(s) 226
AluBI AGCT 2 cut(s) 443, 569
AluI AGCT 2 cut(s) 443, 569
Alw21I GWGCWC 2 cut(s) 298, 379
Alw26I GTCTC 3 cut(s) 80, 254, 409
Alw44I GTGCAC 2 cut(s) 294, 375
AlwI GGATC 2 cut(s) 239, 397
AoxI GGCC 1 cut(s) 90
ApaLI GTGCAC 2 cut(s) 294, 375
ApoI RAATTY 2 cut(s) 690, 723
Asp718I GGTACC 1 cut(s) 358
AspS9I GGNCC 3 cut(s) 617, 659, 710
AsuHPI GGTGA 1 cut(s) 26
AvaII GGWCC 3 cut(s) 617, 659, 710
BaeGI GKGCMC 2 cut(s) 298, 379
BaeI ACNNNNGTAYC 2 cut(s) 350, 383
BanI GGYRCC 1 cut(s) 358
BbsI GAAGAC 1 cut(s) 33
Bbv12I GWGCWC 2 cut(s) 298, 379
BccI CCATC 2 cut(s) 667, 839
BceAI ACGGC 2 cut(s) 105, 296
BciT130I CCWGG 1 cut(s) 228
BcoDI GTCTC 3 cut(s) 80, 254, 409
BcuI ACTAGT 1 cut(s) 484
BfaI CTAG 3 cut(s) 108, 398, 485
Bme1390I CCNGG 1 cut(s) 228
Bme18I GGWCC 3 cut(s) 617, 659, 710
BmeRI GACNNNNNGTC 1 cut(s) 246
BmgT120I GGNCC 3 cut(s) 617, 659, 710
BmiI GGNNCC 3 cut(s) 154, 360, 712
BmrFI CCNGG 1 cut(s) 228
BmsI GCATC 1 cut(s) 641
BpiI GAAGAC 1 cut(s) 33
BpmI CTGGAG 1 cut(s) 579
BsaBI GATNNNNATC 1 cut(s) 500
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 3 cut(s) 73, 227, 347
BsaWI WCCGGW 1 cut(s) 290
BsaXI ACNNNNNCTCC 4 cut(s) 174, 204, 726, 756
Bsc4I CCNNNNNNNGG 2 cut(s) 626, 719
Bse1I ACTGG 2 cut(s) 283, 562
Bse8I GATNNNNATC 1 cut(s) 500
BseBI CCWGG 1 cut(s) 228
BseDI CCNNGG 3 cut(s) 73, 227, 347
BseGI GGATG 2 cut(s) 182, 591
BseJI GATNNNNATC 1 cut(s) 500
BseLI CCNNNNNNNGG 2 cut(s) 626, 719
BseNI ACTGG 2 cut(s) 283, 562
BseRI GAGGAG 1 cut(s) 503
BseSI GKGCMC 2 cut(s) 298, 379
Bsh1236I CGCG 1 cut(s) 578
BshFI GGCC 1 cut(s) 92
BshNI GGYRCC 1 cut(s) 358
BsiHKAI GWGCWC 2 cut(s) 298, 379
BsiSI CCGG 5 cut(s) 291, 337, 531, 615, 732
BslFI GGGAC 1 cut(s) 696
BslI CCNNNNNNNGG 2 cut(s) 626, 719
BsmAI GTCTC 3 cut(s) 80, 254, 409
BsmBI CGTCTC 1 cut(s) 80
BsmFI GGGAC 1 cut(s) 696
BsnI GGCC 1 cut(s) 92
Bso31I GGTCTC 1 cut(s) 254
Bsp1286I GDGCHC 2 cut(s) 298, 379
Bsp143I GATC 4 cut(s) 21, 231, 389, 495
BspACI CCGC 2 cut(s) 340, 578
BspANI GGCC 1 cut(s) 92
BspFNI CGCG 1 cut(s) 578
BspHI TCATGA 2 cut(s) 264, 753
BspLI GGNNCC 3 cut(s) 154, 360, 712
BspPI GGATC 2 cut(s) 239, 397
BspT107I GGYRCC 1 cut(s) 358
BspTNI GGTCTC 1 cut(s) 254
BsrI ACTGG 2 cut(s) 283, 562
BssECI CCNNGG 3 cut(s) 73, 227, 347
BssMI GATC 4 cut(s) 21, 231, 389, 495
BssT1I CCWWGG 2 cut(s) 73, 347
Bst2UI CCWGG 1 cut(s) 228
Bst4CI ACNGT 1 cut(s) 319
Bst6I CTCTTC 1 cut(s) 12
BstAPI GCANNNNNTGC 1 cut(s) 117
BstF5I GGATG 2 cut(s) 182, 591
BstFNI CGCG 1 cut(s) 578
BstKTI GATC 4 cut(s) 24, 234, 392, 498
BstMAI GTCTC 3 cut(s) 80, 254, 409
BstMBI GATC 4 cut(s) 21, 231, 389, 495
BstMWI GCNNNNNNNGC 4 cut(s) 69, 117, 488, 575
BstNI CCWGG 1 cut(s) 228
BstSCI CCNGG 1 cut(s) 226
BstSLI GKGCMC 2 cut(s) 298, 379
BstUI CGCG 1 cut(s) 578
BstV2I GAAGAC 1 cut(s) 33
BstX2I RGATCY 1 cut(s) 231
BstYI RGATCY 1 cut(s) 231
BsuRI GGCC 1 cut(s) 92
BtsCI GGATG 2 cut(s) 182, 591
BtsIMutI CAGTG 2 cut(s) 315, 540
CciI TCATGA 2 cut(s) 264, 753
Cfr13I GGNCC 3 cut(s) 617, 659, 710
Csp6I GTAC 2 cut(s) 205, 359
CviAII CATG 4 cut(s) 11, 265, 517, 754
CviJI RGCY 7 cut(s) 72, 92, 443, 569, 614, 772, 849
CviKI_1 RGCY 7 cut(s) 72, 92, 443, 569, 614, 772, 849
CviQI GTAC 2 cut(s) 205, 359
DpnI GATC 4 cut(s) 23, 233, 391, 497
DpnII GATC 4 cut(s) 21, 231, 389, 495
DriI GACNNNNNGTC 1 cut(s) 246
EaeI YGGCCR 1 cut(s) 90
Eam1104I CTCTTC 1 cut(s) 12
Eam1105I GACNNNNNGTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 12
Eco130I CCWWGG 2 cut(s) 73, 347
Eco31I GGTCTC 1 cut(s) 254
Eco47I GGWCC 3 cut(s) 617, 659, 710
EcoRII CCWGG 1 cut(s) 226
EcoT14I CCWWGG 2 cut(s) 73, 347
ErhI CCWWGG 2 cut(s) 73, 347
Esp3I CGTCTC 1 cut(s) 80
FaeI CATG 4 cut(s) 14, 268, 520, 757
FalI AAGNNNNNCTT 2 cut(s) 784, 816
FaqI GGGAC 1 cut(s) 696
FatI CATG 4 cut(s) 10, 264, 516, 753
FauNDI CATATG 1 cut(s) 95
FokI GGATG 2 cut(s) 189, 598
FspBI CTAG 3 cut(s) 108, 398, 485
GsuI CTGGAG 1 cut(s) 579
HaeIII GGCC 1 cut(s) 92
HapII CCGG 5 cut(s) 291, 337, 531, 615, 732
Hin1II CATG 4 cut(s) 14, 268, 520, 757
HindIII AAGCTT 1 cut(s) 441
HinfI GANTC 3 cut(s) 467, 573, 640
HpaII CCGG 5 cut(s) 291, 337, 531, 615, 732
HphI GGTGA 1 cut(s) 26
Hpy166II GTNNAC 4 cut(s) 296, 377, 700, 745
Hpy188I TCNGA 2 cut(s) 722, 844
Hpy188III TCNNGA 6 cut(s) 42, 174, 265, 474, 627, 754
Hpy8I GTNNAC 4 cut(s) 296, 377, 700, 745
HpyCH4III ACNGT 1 cut(s) 319
HpyCH4IV ACGT 2 cut(s) 323, 603
HpyCH4V TGCA 3 cut(s) 63, 296, 377
HpyF10VI GCNNNNNNNGC 4 cut(s) 69, 117, 488, 575
HpySE526I ACGT 2 cut(s) 323, 603
Hsp92II CATG 4 cut(s) 14, 268, 520, 757
KpnI GGTACC 1 cut(s) 362
Kzo9I GATC 4 cut(s) 21, 231, 389, 495
LmnI GCTCC 1 cut(s) 629
LweI GCATC 1 cut(s) 641
MaeI CTAG 3 cut(s) 108, 398, 485
MaeII ACGT 2 cut(s) 323, 603
MaeIII GTNAC 5 cut(s) 238, 281, 313, 319, 430
MalI GATC 4 cut(s) 23, 233, 391, 497
MboI GATC 4 cut(s) 21, 231, 389, 495
MboII GAAGA 3 cut(s) 29, 38, 690
MfeI CAATTG 1 cut(s) 58
MflI RGATCY 1 cut(s) 231
MhlI GDGCHC 2 cut(s) 298, 379
MluCI AATT 5 cut(s) 45, 58, 563, 690, 723
MmeI TCCRAC 2 cut(s) 485, 700
MnlI CCTC 6 cut(s) 71, 203, 521, 524, 728, 815
MseI TTAA 2 cut(s) 303, 407
MslI CAYNNNNRTG 1 cut(s) 257
MspI CCGG 5 cut(s) 291, 337, 531, 615, 732
MspR9I CCNGG 1 cut(s) 228
MunI CAATTG 1 cut(s) 58
MvaI CCWGG 1 cut(s) 228
MvnI CGCG 1 cut(s) 578
MwoI GCNNNNNNNGC 4 cut(s) 69, 117, 488, 575
NdeI CATATG 1 cut(s) 95
NdeII GATC 4 cut(s) 21, 231, 389, 495
NlaIII CATG 4 cut(s) 14, 268, 520, 757
NlaIV GGNNCC 3 cut(s) 154, 360, 712
NmuCI GTSAC 5 cut(s) 238, 281, 313, 319, 430
PagI TCATGA 2 cut(s) 264, 753
PfeI GAWTC 3 cut(s) 467, 573, 640
Psp6I CCWGG 1 cut(s) 226
PspGI CCWGG 1 cut(s) 226
PspN4I GGNNCC 3 cut(s) 154, 360, 712
PspPI GGNCC 3 cut(s) 617, 659, 710
PsuI RGATCY 1 cut(s) 231
RsaI GTAC 2 cut(s) 206, 360
RsaNI GTAC 2 cut(s) 205, 359
RseI CAYNNNNRTG 1 cut(s) 257
SaqAI TTAA 2 cut(s) 303, 407
Sau3AI GATC 4 cut(s) 21, 231, 389, 495
Sau96I GGNCC 3 cut(s) 617, 659, 710
ScrFI CCNGG 1 cut(s) 228
SduI GDGCHC 2 cut(s) 298, 379
SetI ASST 9 cut(s) 158, 326, 360, 376, 445, 571, 606, 622, 739
SfaNI GCATC 1 cut(s) 641
SinI GGWCC 3 cut(s) 617, 659, 710
SmiMI CAYNNNNRTG 1 cut(s) 257
SpeI ACTAGT 1 cut(s) 484
Sse9I AATT 5 cut(s) 45, 58, 563, 690, 723
SsiI CCGC 2 cut(s) 340, 578
SspI AATATT 2 cut(s) 168, 597
SspMI CTAG 3 cut(s) 108, 398, 485
StyD4I CCNGG 1 cut(s) 226
StyI CCWWGG 2 cut(s) 73, 347
TaaI ACNGT 1 cut(s) 319
TaiI ACGT 2 cut(s) 326, 606
TaqI TCGA 1 cut(s) 24
TasI AATT 5 cut(s) 45, 58, 563, 690, 723
TfiI GAWTC 3 cut(s) 467, 573, 640
Tru1I TTAA 2 cut(s) 303, 407
Tru9I TTAA 2 cut(s) 303, 407
TscAI CASTG 2 cut(s) 322, 547
TseFI GTSAC 5 cut(s) 238, 281, 313, 319, 430
Tsp45I GTSAC 5 cut(s) 238, 281, 313, 319, 430
TspDTI ATGAA 4 cut(s) 151, 459, 653, 742
TspGWI ACGGA 3 cut(s) 222, 779, 797
TspRI CASTG 2 cut(s) 322, 547
VneI GTGCAC 2 cut(s) 294, 375
VpaK11BI GGWCC 3 cut(s) 617, 659, 710
XapI RAATTY 2 cut(s) 690, 723
XspI CTAG 3 cut(s) 108, 398, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.