Rw7G039920

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0006453
Physical Location & Seq
Forward (+)
61682886 .. 61685273
2388 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G039920.1

Sequence Viewer

Length: 696 bp
ATGGCTAACTCACATTCTATTTCATCCCCTAACCTTGGAGTGTCCACTAAGCTATCTACTTTAGAAGAAGCAGATATGTTGGTAGAGCCAATGGGAACGTACGTTCTTGTATTTATGGGGTGTGGGGCTACTCTTGTCAACATAACACATCCTCTTTACGAGACTATGGGCATAGCAATGGTATGGGGTCATCTTTTGATGGCACATACTTATATAGTTGGGCAGAGTACCTATGTATCATTGTCACAGATGACAGCTGCAACACTAGCAAGCCCGACTTTGAGGCTGTTGTTTCAAGACAATATTAACGTGACGGTGACTCTCTACTCTGATTCAGCAACTCACCTACAAGCCCTCATCGGGGAATTCATATCCACTTTTATACTGATGTTCACCATTTGTGGTGTAGCCACTGATCACAGAGCGCAAAGCAAGAACTTATCTGGAGTTGAAATAGGTGTTACAGTTCTGTTCAATGCCACGGTTGCTGGGAGTTTTGGCCCTGCTGCTGTTATTGGTATTTATAAGAATCTTTGCGTCTATCTTTTAGCTCCTATTCTCGGGGCGCTTGCTGCTACTGCATTGTATAGTGTTCTCCGAGTGTCTGACCCAGTGAAATCTGATCCAAATGACAAAACACTTCCACCAATATGGAGTTCAGTCAGAGACTTTTTATTTTATTTTAAGAAGGGCTAA

Protein Analysis

231

Amino Acids

24.8

Weight (kDa)

6.02

Isoelectric Point (pI)

31.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 81 - 196 1.2e-12 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 525
AclWI GGATC 1 cut(s) 617
AcsI RAATTY 1 cut(s) 365
AfaI GTAC 2 cut(s) 101, 229
AfiI CCNNNNNNNGG 4 cut(s) 35, 360, 361, 560
AgsI TTSAA 3 cut(s) 296, 452, 475
AjuI GAANNNNNNNTTGG 2 cut(s) 640, 672
AloI GAACNNNNNNTCC 2 cut(s) 87, 119
AluBI AGCT 3 cut(s) 52, 257, 551
AluI AGCT 3 cut(s) 52, 257, 551
Alw26I GTCTC 2 cut(s) 155, 660
AlwI GGATC 1 cut(s) 617
Ama87I CYCGRG 1 cut(s) 560
AoxI GGCC 1 cut(s) 499
ApeKI GCWGC 3 cut(s) 257, 506, 572
ApoI RAATTY 1 cut(s) 365
AspLEI GCGC 2 cut(s) 427, 568
AspS9I GGNCC 1 cut(s) 500
AsuHPI GGTGA 3 cut(s) 328, 335, 385
AvaI CYCGRG 1 cut(s) 560
BaeI ACNNNNGTAYC 2 cut(s) 219, 252
BbvI GCAGC 3 cut(s) 244, 493, 559
BccI CCATC 1 cut(s) 193
BclI TGATCA 1 cut(s) 415
BcoDI GTCTC 2 cut(s) 155, 660
BfaI CTAG 1 cut(s) 266
BfoI RGCGCY 1 cut(s) 569
BisI GCNGC 3 cut(s) 258, 507, 573
BlsI GCNGC 3 cut(s) 259, 508, 574
BmeT110I CYCGRG 1 cut(s) 560
BmgT120I GGNCC 1 cut(s) 500
BmrI ACTGGG 1 cut(s) 605
BmuI ACTGGG 1 cut(s) 605
BpmI CTGGAG 1 cut(s) 465
BsaJI CCNNGG 2 cut(s) 34, 480
Bsc4I CCNNNNNNNGG 4 cut(s) 35, 360, 361, 560
Bse1I ACTGG 1 cut(s) 611
Bse3DI GCAATG 1 cut(s) 183
BseDI CCNNGG 2 cut(s) 34, 480
BseGI GGATG 2 cut(s) 23, 148
BseLI CCNNNNNNNGG 4 cut(s) 35, 360, 361, 560
BseMI GCAATG 1 cut(s) 183
BseNI ACTGG 1 cut(s) 611
BseXI GCAGC 3 cut(s) 244, 493, 559
BseYI CCCAGC 1 cut(s) 488
BshFI GGCC 1 cut(s) 501
BsiHKCI CYCGRG 1 cut(s) 560
BsiWI CGTACG 1 cut(s) 99
BslI CCNNNNNNNGG 4 cut(s) 35, 360, 361, 560
BsmAI GTCTC 2 cut(s) 155, 660
BsnI GGCC 1 cut(s) 501
BsoBI CYCGRG 1 cut(s) 560
Bsp143I GATC 2 cut(s) 415, 622
BspANI GGCC 1 cut(s) 501
BspPI GGATC 1 cut(s) 617
BsrDI GCAATG 1 cut(s) 183
BsrI ACTGG 1 cut(s) 611
BssECI CCNNGG 2 cut(s) 34, 480
BssMI GATC 2 cut(s) 415, 622
BssT1I CCWWGG 1 cut(s) 34
Bst4CI ACNGT 3 cut(s) 316, 466, 484
BstC8I GCNNGC 2 cut(s) 271, 570
BstDEI CTNAG 1 cut(s) 48
BstDSI CCRYGG 1 cut(s) 480
BstF5I GGATG 2 cut(s) 23, 148
BstH2I RGCGCY 1 cut(s) 569
BstHHI GCGC 2 cut(s) 427, 568
BstKTI GATC 2 cut(s) 418, 625
BstMAI GTCTC 2 cut(s) 155, 660
BstMBI GATC 2 cut(s) 415, 622
BstMWI GCNNNNNNNGC 4 cut(s) 266, 485, 572, 578
BstV1I GCAGC 3 cut(s) 244, 493, 559
BstXI CCANNNNNNTGG 1 cut(s) 651
BsuRI GGCC 1 cut(s) 501
BtgI CCRYGG 1 cut(s) 480
BtsCI GGATG 2 cut(s) 23, 148
BtsIMutI CAGTG 2 cut(s) 411, 618
Cac8I GCNNGC 2 cut(s) 271, 570
CfoI GCGC 2 cut(s) 427, 568
Cfr13I GGNCC 1 cut(s) 500
CseI GACGC 1 cut(s) 526
Csp6I GTAC 2 cut(s) 100, 228
CviQI GTAC 2 cut(s) 100, 228
DdeI CTNAG 1 cut(s) 48
DpnI GATC 2 cut(s) 417, 624
DpnII GATC 2 cut(s) 415, 622
Eco130I CCWWGG 1 cut(s) 34
Eco88I CYCGRG 1 cut(s) 560
EcoRI GAATTC 1 cut(s) 365
EcoT14I CCWWGG 1 cut(s) 34
ErhI CCWWGG 1 cut(s) 34
FalI AAGNNNNNCTT 2 cut(s) 262, 294
FbaI TGATCA 1 cut(s) 415
Fnu4HI GCNGC 3 cut(s) 258, 507, 573
FokI GGATG 2 cut(s) 10, 135
Fsp4HI GCNGC 3 cut(s) 258, 507, 573
FspBI CTAG 1 cut(s) 266
GlaI GCGC 2 cut(s) 426, 567
GluI GCNGC 3 cut(s) 258, 507, 573
GsaI CCCAGC 1 cut(s) 492
GsuI CTGGAG 1 cut(s) 465
HaeII RGCGCY 1 cut(s) 569
HaeIII GGCC 1 cut(s) 501
HgaI GACGC 1 cut(s) 526
HhaI GCGC 2 cut(s) 427, 568
Hin6I GCGC 2 cut(s) 425, 566
HinP1I GCGC 2 cut(s) 425, 566
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HinfI GANTC 3 cut(s) 319, 332, 529
HphI GGTGA 3 cut(s) 328, 335, 385
Hpy166II GTNNAC 3 cut(s) 45, 139, 393
Hpy188I TCNGA 5 cut(s) 331, 599, 607, 622, 665
Hpy188III TCNNGA 2 cut(s) 296, 444
Hpy8I GTNNAC 3 cut(s) 45, 139, 393
HpyAV CCTTC 1 cut(s) 682
HpyCH4III ACNGT 3 cut(s) 316, 466, 484
HpyCH4IV ACGT 3 cut(s) 98, 102, 309
HpyCH4V TGCA 2 cut(s) 260, 581
HpyF10VI GCNNNNNNNGC 4 cut(s) 266, 485, 572, 578
HpyF3I CTNAG 1 cut(s) 48
HpySE526I ACGT 3 cut(s) 98, 102, 309
HspAI GCGC 2 cut(s) 425, 566
Ksp22I TGATCA 1 cut(s) 415
Kzo9I GATC 2 cut(s) 415, 622
LmnI GCTCC 1 cut(s) 556
LpnPI CCDG 4 cut(s) 429, 474, 516, 624
Lsp1109I GCAGC 3 cut(s) 244, 493, 559
MaeI CTAG 1 cut(s) 266
MaeII ACGT 3 cut(s) 98, 102, 309
MaeIII GTNAC 4 cut(s) 243, 310, 316, 460
MalI GATC 2 cut(s) 417, 624
MboI GATC 2 cut(s) 415, 622
MboII GAAGA 1 cut(s) 77
MluCI AATT 1 cut(s) 365
MlyI GAGTC 1 cut(s) 313
MnlI CCTC 3 cut(s) 162, 276, 365
MseI TTAA 2 cut(s) 306, 684
MslI CAYNNNNRTG 2 cut(s) 176, 649
MspA1I CMGCKG 1 cut(s) 257
MwoI GCNNNNNNNGC 4 cut(s) 266, 485, 572, 578
NdeII GATC 2 cut(s) 415, 622
NmuCI GTSAC 3 cut(s) 243, 310, 316
PfeI GAWTC 2 cut(s) 332, 529
Pfl23II CGTACG 1 cut(s) 99
PkrI GCNGC 3 cut(s) 259, 508, 574
PleI GAGTC 1 cut(s) 313
PpsI GAGTC 1 cut(s) 313
PsiI TTATAA 1 cut(s) 525
PspFI CCCAGC 1 cut(s) 488
PspLI CGTACG 1 cut(s) 99
PspPI GGNCC 1 cut(s) 500
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 2 cut(s) 101, 229
RsaNI GTAC 2 cut(s) 100, 228
RseI CAYNNNNRTG 2 cut(s) 176, 649
SaqAI TTAA 2 cut(s) 306, 684
SatI GCNGC 3 cut(s) 258, 507, 573
Sau3AI GATC 2 cut(s) 415, 622
Sau96I GGNCC 1 cut(s) 500
SchI GAGTC 1 cut(s) 313
SmiMI CAYNNNNRTG 2 cut(s) 176, 649
Sse9I AATT 1 cut(s) 365
SspI AATATT 1 cut(s) 304
SspMI CTAG 1 cut(s) 266
StyI CCWWGG 1 cut(s) 34
TaaI ACNGT 3 cut(s) 316, 466, 484
TaiI ACGT 3 cut(s) 101, 105, 312
TasI AATT 1 cut(s) 365
TfiI GAWTC 2 cut(s) 332, 529
Tru1I TTAA 2 cut(s) 306, 684
Tru9I TTAA 2 cut(s) 306, 684
TscAI CASTG 2 cut(s) 418, 618
TseFI GTSAC 3 cut(s) 243, 310, 316
TseI GCWGC 3 cut(s) 257, 506, 572
Tsp45I GTSAC 3 cut(s) 243, 310, 316
TspDTI ATGAA 2 cut(s) 12, 358
TspRI CASTG 2 cut(s) 418, 618
XapI RAATTY 1 cut(s) 365
XspI CTAG 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.