AT5G43180

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004770
Physical Location & Seq
Reverse (-)
17336291 .. 17337884
1594 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G43180.1

Sequence Viewer

Length: 720 bp
ATGAGAATGAAGATCGACACAGACAGCATTGTAGTGCCATTGAGTTTGTTGATAGCTGTGGGATACCATGTCTTTCTATGGAACACCTTCAAGCACAATCCTTCTCGTACTTCACTTGGCATTGATTCCTCAAAACGCAAATCTTGGTTCCGTGACATCAAAGAGGGTGACGATAAGACTGGGATGTTGGCCGTACAAAGCTTGAGGAACAAAAAAATGGTGACAATTCTAACCGCTACAATCGCCATCCTTATCTTCCTTTCTCTAGCTGCGGTAACTAACAACGCATTTAAAGCCAGTCATCTCATCACTGCCGCTGATGACATCTTCTTTGGCTCACAAAATGCTAAAATCTTTGTCCTAAAATATGCCTCAGCCTCGTTACTTCTTGCAGCAAGCTTCTTCTTTAGCTCAATTGCCTTGAGTTATTTAATGGATGCAATTTTCCTTATCAACGCAATAGCTAAAAAGCATGAAGGAGACTGTGACTGCGCTTATGATATCACGGGGACAAGTTCGTTTAGGGAGTATACAAGATTGGTGTTGGAGAGAGGGTTTTTTATGGCTATGGTTGGGAACAGAGTTATGTGTGTTTCTATACCGTTGTTGCTTTGGATGTTTGGACCGTTACCGGTGCTCGCATCTTCATTAGGCCTTGTTTGGGTGTTGTATCAGTTTGATTTTCCGTCAACCGCTAAAATTTCTGTTTGTAAAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

239

Amino Acids

26.54

Weight (kDa)

9.28

Isoelectric Point (pI)

27.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 10 - 231 5.6e-54 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 530
AciI CCGC 4 cut(s) 234, 272, 315, 693
AcoI YGGCCR 1 cut(s) 189
AcsI RAATTY 1 cut(s) 699
AfaI GTAC 2 cut(s) 109, 195
AfiI CCNNNNNNNGG 1 cut(s) 661
AgeI ACCGGT 1 cut(s) 631
AgsI TTSAA 1 cut(s) 91
AluBI AGCT 6 cut(s) 56, 201, 269, 399, 411, 464
AluI AGCT 6 cut(s) 56, 201, 269, 399, 411, 464
Alw21I GWGCWC 1 cut(s) 639
Alw26I GTCTC 1 cut(s) 474
AoxI GGCC 2 cut(s) 189, 652
ApeKI GCWGC 2 cut(s) 269, 392
ApoI RAATTY 1 cut(s) 699
ArsI GACNNNNNNTTYG 2 cut(s) 314, 346
AsiGI ACCGGT 1 cut(s) 631
Asp700I GAANNNNTTC 1 cut(s) 86
AspLEI GCGC 1 cut(s) 494
AspS9I GGNCC 1 cut(s) 623
AsuHPI GGTGA 2 cut(s) 179, 232
AvaII GGWCC 1 cut(s) 623
Bbv12I GWGCWC 1 cut(s) 639
BbvCI CCTCAGC 1 cut(s) 373
BbvI GCAGC 2 cut(s) 256, 404
BccI CCATC 1 cut(s) 254
BceAI ACGGC 1 cut(s) 176
BciVI GTATCC 1 cut(s) 56
BcoDI GTCTC 1 cut(s) 474
BfaI CTAG 1 cut(s) 266
BfuI GTATCC 1 cut(s) 56
BisI GCNGC 3 cut(s) 270, 315, 393
BlsI GCNGC 3 cut(s) 271, 316, 394
Bme18I GGWCC 1 cut(s) 623
BmgT120I GGNCC 1 cut(s) 623
BmiI GGNNCC 1 cut(s) 149
BmrI ACTGGG 1 cut(s) 189
BmsI GCATC 2 cut(s) 427, 650
BmuI ACTGGG 1 cut(s) 189
Bpu10I CCTNAGC 1 cut(s) 373
BpuEI CTTGAG 2 cut(s) 223, 442
BsaWI WCCGGW 1 cut(s) 631
BsaXI ACNNNNNCTCC 2 cut(s) 539, 569
Bsc4I CCNNNNNNNGG 1 cut(s) 661
Bse118I RCCGGY 1 cut(s) 631
Bse1I ACTGG 2 cut(s) 184, 297
BseGI GGATG 4 cut(s) 189, 246, 442, 621
BseLI CCNNNNNNNGG 1 cut(s) 661
BseMII CTCAG 1 cut(s) 387
BseNI ACTGG 2 cut(s) 184, 297
BseXI GCAGC 2 cut(s) 256, 404
BshFI GGCC 2 cut(s) 191, 654
BshTI ACCGGT 1 cut(s) 631
BsiHKAI GWGCWC 1 cut(s) 639
BsiSI CCGG 1 cut(s) 632
BslFI GGGAC 1 cut(s) 523
BslI CCNNNNNNNGG 1 cut(s) 661
BsmAI GTCTC 1 cut(s) 474
BsmFI GGGAC 1 cut(s) 523
BsnI GGCC 2 cut(s) 191, 654
Bsp1286I GDGCHC 1 cut(s) 639
Bsp143I GATC 1 cut(s) 12
BspACI CCGC 4 cut(s) 234, 272, 315, 693
BspANI GGCC 2 cut(s) 191, 654
BspCNI CTCAG 1 cut(s) 386
BspLI GGNNCC 1 cut(s) 149
BsrFI RCCGGY 1 cut(s) 631
BsrI ACTGG 2 cut(s) 184, 297
BssAI RCCGGY 1 cut(s) 631
BssMI GATC 1 cut(s) 12
BssNAI GTATAC 1 cut(s) 531
Bst1107I GTATAC 1 cut(s) 531
Bst4CI ACNGT 3 cut(s) 485, 603, 627
BstC8I GCNNGC 2 cut(s) 397, 639
BstDEI CTNAG 1 cut(s) 373
BstF5I GGATG 4 cut(s) 189, 246, 442, 621
BstHHI GCGC 1 cut(s) 494
BstKTI GATC 1 cut(s) 15
BstMAI GTCTC 1 cut(s) 474
BstMBI GATC 1 cut(s) 12
BstMWI GCNNNNNNNGC 2 cut(s) 242, 293
BstV1I GCAGC 2 cut(s) 256, 404
BstZ17I GTATAC 1 cut(s) 531
BsuI GTATCC 1 cut(s) 56
BsuRI GGCC 2 cut(s) 191, 654
BtsCI GGATG 4 cut(s) 189, 246, 442, 621
BtsI GCAGTG 1 cut(s) 309
BtsIMutI CAGTG 1 cut(s) 309
Cac8I GCNNGC 2 cut(s) 397, 639
CfoI GCGC 1 cut(s) 494
Cfr10I RCCGGY 1 cut(s) 631
Cfr13I GGNCC 1 cut(s) 623
Csp6I GTAC 2 cut(s) 108, 194
CspAI ACCGGT 1 cut(s) 631
CviAII CATG 2 cut(s) 68, 473
CviQI GTAC 2 cut(s) 108, 194
DdeI CTNAG 1 cut(s) 373
DpnI GATC 1 cut(s) 14
DpnII GATC 1 cut(s) 12
DraI TTTAAA 1 cut(s) 292
EaeI YGGCCR 1 cut(s) 189
Eco147I AGGCCT 1 cut(s) 654
Eco32I GATATC 1 cut(s) 502
Eco47I GGWCC 1 cut(s) 623
EcoRV GATATC 1 cut(s) 502
FaeI CATG 2 cut(s) 71, 476
FaqI GGGAC 1 cut(s) 523
FatI CATG 2 cut(s) 67, 472
FblI GTMKAC 1 cut(s) 530
Fnu4HI GCNGC 3 cut(s) 270, 315, 393
FokI GGATG 4 cut(s) 196, 233, 449, 628
Fsp4HI GCNGC 3 cut(s) 270, 315, 393
FspBI CTAG 1 cut(s) 266
GlaI GCGC 1 cut(s) 493
GluI GCNGC 3 cut(s) 270, 315, 393
HaeIII GGCC 2 cut(s) 191, 654
HapII CCGG 1 cut(s) 632
HhaI GCGC 1 cut(s) 494
Hin1II CATG 2 cut(s) 71, 476
Hin6I GCGC 1 cut(s) 492
HinP1I GCGC 1 cut(s) 492
HincII GTYRAC 1 cut(s) 690
HindII GTYRAC 1 cut(s) 690
HindIII AAGCTT 2 cut(s) 199, 397
HinfI GANTC 1 cut(s) 125
HpaII CCGG 1 cut(s) 632
HphI GGTGA 2 cut(s) 179, 232
Hpy166II GTNNAC 2 cut(s) 531, 690
Hpy8I GTNNAC 2 cut(s) 531, 690
HpyAV CCTTC 3 cut(s) 97, 111, 470
HpyCH4III ACNGT 3 cut(s) 485, 603, 627
HpyCH4V TGCA 2 cut(s) 392, 440
HpyF10VI GCNNNNNNNGC 2 cut(s) 242, 293
HpyF3I CTNAG 1 cut(s) 373
Hsp92II CATG 2 cut(s) 71, 476
HspAI GCGC 1 cut(s) 492
Kzo9I GATC 1 cut(s) 12
LpnPI CCDG 3 cut(s) 165, 310, 645
Lsp1109I GCAGC 2 cut(s) 256, 404
LweI GCATC 2 cut(s) 427, 650
MaeI CTAG 1 cut(s) 266
MaeIII GTNAC 7 cut(s) 152, 167, 220, 274, 381, 485, 627
MalI GATC 1 cut(s) 14
MboI GATC 1 cut(s) 12
MboII GAAGA 5 cut(s) 22, 247, 319, 394, 636
MfeI CAATTG 1 cut(s) 414
MhlI GDGCHC 1 cut(s) 639
MluCI AATT 4 cut(s) 225, 414, 441, 699
MmeI TCCRAC 1 cut(s) 525
MnlI CCTC 6 cut(s) 139, 157, 198, 382, 388, 545
MroXI GAANNNNTTC 1 cut(s) 86
MseI TTAA 2 cut(s) 291, 431
MslI CAYNNNNRTG 1 cut(s) 32
MspA1I CMGCKG 1 cut(s) 317
MspI CCGG 1 cut(s) 632
MunI CAATTG 1 cut(s) 414
MwoI GCNNNNNNNGC 2 cut(s) 242, 293
NdeII GATC 1 cut(s) 12
NlaIII CATG 2 cut(s) 71, 476
NlaIV GGNNCC 1 cut(s) 149
NmuCI GTSAC 4 cut(s) 152, 167, 220, 485
PceI AGGCCT 1 cut(s) 654
PdmI GAANNNNTTC 1 cut(s) 86
PfeI GAWTC 1 cut(s) 125
PinAI ACCGGT 1 cut(s) 631
PkrI GCNGC 3 cut(s) 271, 316, 394
PspN4I GGNNCC 1 cut(s) 149
PspPI GGNCC 1 cut(s) 623
RsaI GTAC 2 cut(s) 109, 195
RsaNI GTAC 2 cut(s) 108, 194
RseI CAYNNNNRTG 1 cut(s) 32
SaqAI TTAA 2 cut(s) 291, 431
SatI GCNGC 3 cut(s) 270, 315, 393
Sau3AI GATC 1 cut(s) 12
Sau96I GGNCC 1 cut(s) 623
SduI GDGCHC 1 cut(s) 639
SetI ASST 7 cut(s) 58, 89, 203, 271, 401, 413, 466
SfaNI GCATC 2 cut(s) 427, 650
SinI GGWCC 1 cut(s) 623
SmiMI CAYNNNNRTG 1 cut(s) 32
SmlI CTYRAG 2 cut(s) 202, 421
SmoI CTYRAG 2 cut(s) 202, 421
Sse9I AATT 4 cut(s) 225, 414, 441, 699
SseBI AGGCCT 1 cut(s) 654
SsiI CCGC 4 cut(s) 234, 272, 315, 693
SspMI CTAG 1 cut(s) 266
StuI AGGCCT 1 cut(s) 654
TaaI ACNGT 3 cut(s) 485, 603, 627
TaqI TCGA 1 cut(s) 15
TasI AATT 4 cut(s) 225, 414, 441, 699
TauI GCSGC 1 cut(s) 317
TfiI GAWTC 1 cut(s) 125
Tru1I TTAA 2 cut(s) 291, 431
Tru9I TTAA 2 cut(s) 291, 431
TscAI CASTG 1 cut(s) 316
TseFI GTSAC 4 cut(s) 152, 167, 220, 485
TseI GCWGC 2 cut(s) 269, 392
Tsp45I GTSAC 4 cut(s) 152, 167, 220, 485
TspDTI ATGAA 3 cut(s) 23, 489, 636
TspGWI ACGGA 2 cut(s) 140, 675
TspRI CASTG 1 cut(s) 316
VpaK11BI GGWCC 1 cut(s) 623
XapI RAATTY 1 cut(s) 699
XmiI GTMKAC 1 cut(s) 530
XmnI GAANNNNTTC 1 cut(s) 86
XspI CTAG 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.