Rroxscaffold_6G00419550

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004770
Physical Location & Seq
Forward (+)
40979664 .. 40981060
1397 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00419550.1

Sequence Viewer

Length: 660 bp
ATGGCGGTGATTGTTTATATAGACACCATATTGGTCCCCATTAGTCTCTTCCTTACGGTGGGTTACCATGCTTATCTCTGGCATAGCCTCAAGAACAAACCCTCTCATACTACCATTGGAATCAACATATTGAAGAGGAGAGCATGGTTTCTAGATATCAAAGGGGGTGATGAAGATAAGATGGGTATGTTGGCTGTCCAAAGCTTGAGAAACACTCTAATGGGAGCCACACTCACAGCTGTTATAACAATTCTTGTTGTAGTTTCATTAGCTGCTCTAATCAACAACGCCTATAGTGCAACACACATCTTCAGCAGTGTGATATTCGGGTCACAATCCCCTTGGATATTTGCTCTGAAGTATGGCTCAGCAGCACTGGCATTGTCAATTAGCTTCCTCTGCAGTTCTATGGCAATCGCATTCTTGATTGATGCGAATTTCCTGATTAATGCCTCCGGTGATCATGAGTTCTCGTCCTCGTCTTCAGAGAACACACACAAAGCATATTTGAGAAAGGGTTTGTTTTGGCTCTTATTAGCAACAGAATGTTGTGCATCACTTTTCCGGTGCTGTTATGGATGCTTGGGCCGATACCGGCTTGTTTGTCGTCGATGGCACTGGTTTGGGGGCTTTATGGGCTTGATTTTGCTCGAAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

219

Amino Acids

24.26

Weight (kDa)

9.01

Isoelectric Point (pI)

49.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 10 - 165 5.5e-36 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 245
AciI CCGC 1 cut(s) 5
AcsI RAATTY 1 cut(s) 436
AcuI CTGAAG 3 cut(s) 295, 377, 468
AfiI CCNNNNNNNGG 1 cut(s) 58
AgsI TTSAA 1 cut(s) 133
AluBI AGCT 4 cut(s) 204, 239, 272, 393
AluI AGCT 4 cut(s) 204, 239, 272, 393
Alw26I GTCTC 1 cut(s) 50
AoxI GGCC 1 cut(s) 586
ApeKI GCWGC 2 cut(s) 272, 371
ApoI RAATTY 1 cut(s) 436
AseI ATTAAT 1 cut(s) 447
AspS9I GGNCC 2 cut(s) 34, 586
AsuHPI GGTGA 3 cut(s) 19, 179, 470
AvaII GGWCC 1 cut(s) 34
BbsI GAAGAC 1 cut(s) 474
BbvI GCAGC 2 cut(s) 259, 383
BccI CCATC 2 cut(s) 175, 606
BclI TGATCA 1 cut(s) 460
BcoDI GTCTC 1 cut(s) 50
BfaI CTAG 1 cut(s) 152
BfmI CTRYAG 2 cut(s) 292, 400
BisI GCNGC 2 cut(s) 273, 372
BlpI GCTNAGC 1 cut(s) 367
BlsI GCNGC 2 cut(s) 274, 373
Bme18I GGWCC 1 cut(s) 34
BmgT120I GGNCC 2 cut(s) 34, 586
BmiI GGNNCC 2 cut(s) 36, 226
BmsI GCATC 3 cut(s) 421, 563, 569
BpiI GAAGAC 1 cut(s) 474
BplI GAGNNNNNCTC 4 cut(s) 199, 231, 216, 248
Bpu1102I GCTNAGC 1 cut(s) 367
BpuEI CTTGAG 2 cut(s) 74, 226
BsaJI CCNNGG 1 cut(s) 341
BsaWI WCCGGW 2 cut(s) 455, 564
Bsc4I CCNNNNNNNGG 1 cut(s) 58
Bse118I RCCGGY 1 cut(s) 594
Bse1I ACTGG 2 cut(s) 381, 623
BseDI CCNNGG 1 cut(s) 341
BseGI GGATG 1 cut(s) 584
BseLI CCNNNNNNNGG 1 cut(s) 58
BseMII CTCAG 1 cut(s) 381
BseNI ACTGG 2 cut(s) 381, 623
BseRI GAGGAG 1 cut(s) 151
BseXI GCAGC 2 cut(s) 259, 383
BshFI GGCC 1 cut(s) 588
BsiSI CCGG 3 cut(s) 456, 565, 595
BslFI GGGAC 1 cut(s) 20
BslI CCNNNNNNNGG 1 cut(s) 58
BsmAI GTCTC 1 cut(s) 50
BsmFI GGGAC 1 cut(s) 20
BsmI GAATGC 1 cut(s) 419
BsnI GGCC 1 cut(s) 588
Bsp143I GATC 1 cut(s) 460
Bsp1720I GCTNAGC 1 cut(s) 367
BspACI CCGC 1 cut(s) 5
BspANI GGCC 1 cut(s) 588
BspCNI CTCAG 1 cut(s) 380
BspHI TCATGA 1 cut(s) 463
BspLI GGNNCC 2 cut(s) 36, 226
BspMAI CTGCAG 1 cut(s) 404
BsrFI RCCGGY 1 cut(s) 594
BsrI ACTGG 2 cut(s) 381, 623
BssAI RCCGGY 1 cut(s) 594
BssECI CCNNGG 1 cut(s) 341
BssMI GATC 1 cut(s) 460
BssT1I CCWWGG 1 cut(s) 341
Bst4CI ACNGT 1 cut(s) 58
Bst6I CTCTTC 2 cut(s) 53, 128
BstDEI CTNAG 1 cut(s) 367
BstEII GGTNACC 1 cut(s) 62
BstF5I GGATG 1 cut(s) 584
BstKTI GATC 1 cut(s) 463
BstMAI GTCTC 1 cut(s) 50
BstMBI GATC 1 cut(s) 460
BstMWI GCNNNNNNNGC 4 cut(s) 296, 377, 399, 636
BstPI GGTNACC 1 cut(s) 62
BstSFI CTRYAG 2 cut(s) 292, 400
BstV1I GCAGC 2 cut(s) 259, 383
BstV2I GAAGAC 1 cut(s) 474
BsuRI GGCC 1 cut(s) 588
BtsCI GGATG 1 cut(s) 584
BtsI GCAGTG 1 cut(s) 322
BtsIMutI CAGTG 3 cut(s) 322, 374, 616
CciI TCATGA 1 cut(s) 463
Cfr10I RCCGGY 1 cut(s) 594
Cfr13I GGNCC 2 cut(s) 34, 586
CviAII CATG 3 cut(s) 68, 144, 464
DdeI CTNAG 1 cut(s) 367
DpnI GATC 1 cut(s) 462
DpnII GATC 1 cut(s) 460
Eam1104I CTCTTC 2 cut(s) 53, 128
EarI CTCTTC 2 cut(s) 53, 128
Eco130I CCWWGG 1 cut(s) 341
Eco32I GATATC 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 34
Eco57I CTGAAG 3 cut(s) 295, 377, 468
Eco91I GGTNACC 1 cut(s) 62
EcoO65I GGTNACC 1 cut(s) 62
EcoRV GATATC 1 cut(s) 157
EcoT14I CCWWGG 1 cut(s) 341
ErhI CCWWGG 1 cut(s) 341
FaeI CATG 3 cut(s) 71, 147, 467
FaqI GGGAC 1 cut(s) 20
FatI CATG 3 cut(s) 67, 143, 463
FbaI TGATCA 1 cut(s) 460
Fnu4HI GCNGC 2 cut(s) 273, 372
FokI GGATG 1 cut(s) 591
Fsp4HI GCNGC 2 cut(s) 273, 372
FspBI CTAG 1 cut(s) 152
GluI GCNGC 2 cut(s) 273, 372
HaeIII GGCC 1 cut(s) 588
HapII CCGG 3 cut(s) 456, 565, 595
Hin1II CATG 3 cut(s) 71, 147, 467
HindIII AAGCTT 1 cut(s) 202
HinfI GANTC 1 cut(s) 120
HpaII CCGG 3 cut(s) 456, 565, 595
HphI GGTGA 3 cut(s) 19, 179, 470
Hpy188I TCNGA 2 cut(s) 357, 487
Hpy188III TCNNGA 5 cut(s) 91, 152, 424, 442, 464
Hpy99I CGWCG 1 cut(s) 612
HpyCH4III ACNGT 1 cut(s) 58
HpyCH4V TGCA 3 cut(s) 299, 402, 554
HpyF10VI GCNNNNNNNGC 4 cut(s) 296, 377, 399, 636
HpyF3I CTNAG 1 cut(s) 367
Hsp92II CATG 3 cut(s) 71, 147, 467
Ksp22I TGATCA 1 cut(s) 460
Kzo9I GATC 1 cut(s) 460
LmnI GCTCC 1 cut(s) 224
LpnPI CCDG 7 cut(s) 64, 362, 455, 469, 578, 604, 608
Lsp1109I GCAGC 2 cut(s) 259, 383
LweI GCATC 3 cut(s) 421, 563, 569
MaeI CTAG 1 cut(s) 152
MaeIII GTNAC 2 cut(s) 62, 330
MalI GATC 1 cut(s) 462
MboI GATC 1 cut(s) 460
MboII GAAGA 5 cut(s) 40, 145, 185, 301, 474
MluCI AATT 3 cut(s) 249, 387, 436
MnlI CCTC 6 cut(s) 98, 112, 129, 407, 463, 487
MseI TTAA 1 cut(s) 447
MslI CAYNNNNRTG 1 cut(s) 218
MspA1I CMGCKG 1 cut(s) 239
MspI CCGG 3 cut(s) 456, 565, 595
Mva1269I GAATGC 1 cut(s) 419
MwoI GCNNNNNNNGC 4 cut(s) 296, 377, 399, 636
NdeII GATC 1 cut(s) 460
NlaIII CATG 3 cut(s) 71, 147, 467
NlaIV GGNNCC 2 cut(s) 36, 226
NmuCI GTSAC 1 cut(s) 330
PagI TCATGA 1 cut(s) 463
PctI GAATGC 1 cut(s) 419
PfeI GAWTC 1 cut(s) 120
PkrI GCNGC 2 cut(s) 274, 373
PshBI ATTAAT 1 cut(s) 447
PsiI TTATAA 1 cut(s) 245
PspEI GGTNACC 1 cut(s) 62
PspN4I GGNNCC 2 cut(s) 36, 226
PspPI GGNCC 2 cut(s) 34, 586
PstI CTGCAG 1 cut(s) 404
PvuII CAGCTG 1 cut(s) 239
RseI CAYNNNNRTG 1 cut(s) 218
SaqAI TTAA 1 cut(s) 447
SatI GCNGC 2 cut(s) 273, 372
Sau3AI GATC 1 cut(s) 460
Sau96I GGNCC 2 cut(s) 34, 586
SetI ASST 4 cut(s) 206, 241, 274, 395
SfaNI GCATC 3 cut(s) 421, 563, 569
SfcI CTRYAG 2 cut(s) 292, 400
SinI GGWCC 1 cut(s) 34
SmiMI CAYNNNNRTG 1 cut(s) 218
SmlI CTYRAG 2 cut(s) 89, 205
SmoI CTYRAG 2 cut(s) 89, 205
Sse9I AATT 3 cut(s) 249, 387, 436
SsiI CCGC 1 cut(s) 5
SspMI CTAG 1 cut(s) 152
StyI CCWWGG 1 cut(s) 341
TaaI ACNGT 1 cut(s) 58
TaqI TCGA 2 cut(s) 610, 651
TasI AATT 3 cut(s) 249, 387, 436
TfiI GAWTC 1 cut(s) 120
Tru1I TTAA 1 cut(s) 447
Tru9I TTAA 1 cut(s) 447
TscAI CASTG 3 cut(s) 322, 381, 623
TseFI GTSAC 1 cut(s) 330
TseI GCWGC 2 cut(s) 272, 371
Tsp45I GTSAC 1 cut(s) 330
TspDTI ATGAA 2 cut(s) 186, 255
TspRI CASTG 3 cut(s) 322, 381, 623
VpaK11BI GGWCC 1 cut(s) 34
VspI ATTAAT 1 cut(s) 447
XapI RAATTY 1 cut(s) 436
XbaI TCTAGA 1 cut(s) 151
XspI CTAG 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.