MD04G1175400.v1.1

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0004770
Physical Location & Seq
Forward (+)
26644516 .. 26645860
1345 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1175400.v1.1.491

Sequence Viewer

Length: 675 bp
ATGGGAGTGATCGCTTATCTAGACAACGTTTTGGTTCCCATGAGCCTCTTTCTCACAGTTGGTTACCATGCCTATCTATGGCACAACCTCAAGAACAAGCCCTCTCGTACCACAATTGGGATCAATATGCTGAAGAGGAGAGCTTGGTTCCTAGAGCTAGATGGGGGTGATGATAAGAAAGGCATGTTAGCAGTCCAAAGCTTGAGAAATACTCTTATGGGGACAACACTCACAGCCACAGTAGCAATTCTTATTCAAGTCTCGCTGGCAGCTCTAATAAACAATTCCTATAGTGCAACGCACCTCTTCAGCAATACCTTTTTTGGGTCACAATCCACCAAGATTTTTGCCTTAAAATATGGCTCCGCAGCTTTGTCTTTGTCAGTTTGCTTCTTGTGCAGCTCCATGGCGATTGCGTTTTTGATCGATGCCAACTTCTTGATCAATGCTTGTGATCAGGAGTTCTCATCTTCTGGACACGCAAGAACCATACTTGAGAAAGGGTTTATTTTGGCTCTTGTAAGCAACAGGATGCTGTGCATCACTTTTCCTATGCTGTTGTGGATGCTTGGTCCTGTCCCGGTTTGGTTGTCATCTTTGGCGATGGTTTGGGGGCTTTATGGGCTGGATTTTCCTGGCAAGTTCAATAAAAGCAATAAGCAAAATCTCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

225

Amino Acids

24.68

Weight (kDa)

9.36

Isoelectric Point (pI)

42.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 10 - 211 4.8e-52 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 366
AclI AACGTT 1 cut(s) 27
AclWI GGATC 1 cut(s) 128
AcuI CTGAAG 2 cut(s) 152, 292
AfaI GTAC 1 cut(s) 109
AfiI CCNNNNNNNGG 3 cut(s) 78, 117, 324
AgsI TTSAA 2 cut(s) 257, 646
AjnI CCWGG 1 cut(s) 634
AluBI AGCT 6 cut(s) 143, 157, 201, 272, 371, 402
AluI AGCT 6 cut(s) 143, 157, 201, 272, 371, 402
Alw26I GTCTC 1 cut(s) 265
AlwI GGATC 1 cut(s) 128
ApeKI GCWGC 3 cut(s) 269, 368, 399
AspS9I GGNCC 1 cut(s) 572
AsuC2I CCSGG 1 cut(s) 581
AsuHPI GGTGA 1 cut(s) 179
AvaII GGWCC 1 cut(s) 572
BbvI GCAGC 3 cut(s) 281, 380, 411
BccI CCATC 2 cut(s) 155, 598
BciT130I CCWGG 1 cut(s) 636
BclI TGATCA 2 cut(s) 441, 454
BcnI CCSGG 1 cut(s) 581
BcoDI GTCTC 1 cut(s) 265
BfaI CTAG 3 cut(s) 20, 152, 158
BfmI CTRYAG 1 cut(s) 289
BisI GCNGC 3 cut(s) 270, 369, 400
BlsI GCNGC 3 cut(s) 271, 370, 401
Bme1390I CCNGG 2 cut(s) 581, 636
Bme18I GGWCC 1 cut(s) 572
BmgT120I GGNCC 1 cut(s) 572
BmiI GGNNCC 3 cut(s) 36, 149, 364
BmrFI CCNGG 2 cut(s) 581, 636
BmsI GCATC 4 cut(s) 418, 522, 549, 555
BplI GAGNNNNNCTC 2 cut(s) 196, 228
BpuEI CTTGAG 3 cut(s) 74, 223, 515
BpuMI CCSGG 1 cut(s) 581
Bsa29I ATCGAT 1 cut(s) 426
BsaJI CCNNGG 1 cut(s) 405
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 117, 324
BseBI CCWGG 1 cut(s) 636
BseCI ATCGAT 1 cut(s) 426
BseDI CCNNGG 1 cut(s) 405
BseGI GGATG 2 cut(s) 537, 570
BseLI CCNNNNNNNGG 3 cut(s) 78, 117, 324
BseRI GAGGAG 1 cut(s) 151
BseXI GCAGC 3 cut(s) 281, 380, 411
BsgI GTGCAG 1 cut(s) 418
BshVI ATCGAT 1 cut(s) 426
BsiSI CCGG 1 cut(s) 581
BslFI GGGAC 2 cut(s) 235, 563
BslI CCNNNNNNNGG 3 cut(s) 78, 117, 324
BsmAI GTCTC 1 cut(s) 265
BsmFI GGGAC 2 cut(s) 235, 563
Bsp143I GATC 5 cut(s) 9, 120, 423, 441, 454
Bsp19I CCATGG 1 cut(s) 405
BspACI CCGC 1 cut(s) 366
BspDI ATCGAT 1 cut(s) 426
BspLI GGNNCC 3 cut(s) 36, 149, 364
BspPI GGATC 1 cut(s) 128
BssECI CCNNGG 1 cut(s) 405
BssMI GATC 5 cut(s) 9, 120, 423, 441, 454
BssT1I CCWWGG 1 cut(s) 405
Bst2UI CCWGG 1 cut(s) 636
Bst4CI ACNGT 2 cut(s) 58, 241
Bst6I CTCTTC 2 cut(s) 128, 311
BstC8I GCNNGC 1 cut(s) 267
BstDEI CTNAG 1 cut(s) 668
BstDSI CCRYGG 1 cut(s) 405
BstEII GGTNACC 1 cut(s) 62
BstF5I GGATG 2 cut(s) 537, 570
BstKTI GATC 5 cut(s) 12, 123, 426, 444, 457
BstMAI GTCTC 1 cut(s) 265
BstMBI GATC 5 cut(s) 9, 120, 423, 441, 454
BstMWI GCNNNNNNNGC 3 cut(s) 242, 396, 622
BstNI CCWGG 1 cut(s) 636
BstNSI RCATGY 1 cut(s) 187
BstPI GGTNACC 1 cut(s) 62
BstSCI CCNGG 2 cut(s) 579, 634
BstSFI CTRYAG 1 cut(s) 289
BstV1I GCAGC 3 cut(s) 281, 380, 411
Bsu15I ATCGAT 1 cut(s) 426
BsuTUI ATCGAT 1 cut(s) 426
BtgI CCRYGG 1 cut(s) 405
BtgZI GCGATG 1 cut(s) 617
BtsCI GGATG 2 cut(s) 537, 570
Cac8I GCNNGC 1 cut(s) 267
Cfr13I GGNCC 1 cut(s) 572
ClaI ATCGAT 1 cut(s) 426
Csp6I GTAC 1 cut(s) 108
CviAII CATG 4 cut(s) 40, 68, 184, 406
CviQI GTAC 1 cut(s) 108
DdeI CTNAG 1 cut(s) 668
DpnI GATC 5 cut(s) 11, 122, 425, 443, 456
DpnII GATC 5 cut(s) 9, 120, 423, 441, 454
Eam1104I CTCTTC 2 cut(s) 128, 311
EarI CTCTTC 2 cut(s) 128, 311
Eco130I CCWWGG 1 cut(s) 405
Eco47I GGWCC 1 cut(s) 572
Eco57I CTGAAG 2 cut(s) 152, 292
Eco91I GGTNACC 1 cut(s) 62
EcoO65I GGTNACC 1 cut(s) 62
EcoRII CCWGG 1 cut(s) 634
EcoT14I CCWWGG 1 cut(s) 405
ErhI CCWWGG 1 cut(s) 405
FaeI CATG 4 cut(s) 43, 71, 187, 409
FaqI GGGAC 2 cut(s) 235, 563
FatI CATG 4 cut(s) 39, 67, 183, 405
FbaI TGATCA 2 cut(s) 441, 454
Fnu4HI GCNGC 3 cut(s) 270, 369, 400
FokI GGATG 2 cut(s) 544, 577
Fsp4HI GCNGC 3 cut(s) 270, 369, 400
FspBI CTAG 3 cut(s) 20, 152, 158
GluI GCNGC 3 cut(s) 270, 369, 400
HapII CCGG 1 cut(s) 581
Hin1II CATG 4 cut(s) 43, 71, 187, 409
HindIII AAGCTT 1 cut(s) 199
HpaII CCGG 1 cut(s) 581
HphI GGTGA 1 cut(s) 179
Hpy188III TCNNGA 5 cut(s) 20, 91, 439, 458, 474
HpyCH4III ACNGT 2 cut(s) 58, 241
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 3 cut(s) 296, 399, 540
HpyF10VI GCNNNNNNNGC 3 cut(s) 242, 396, 622
HpyF3I CTNAG 1 cut(s) 668
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 4 cut(s) 43, 71, 187, 409
Ksp22I TGATCA 2 cut(s) 441, 454
Kzo9I GATC 5 cut(s) 9, 120, 423, 441, 454
LmnI GCTCC 2 cut(s) 368, 407
LpnPI CCDG 9 cut(s) 251, 443, 459, 514, 588, 594, 611, 621, 648
Lsp1109I GCAGC 3 cut(s) 281, 380, 411
LweI GCATC 4 cut(s) 418, 522, 549, 555
MaeI CTAG 3 cut(s) 20, 152, 158
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 2 cut(s) 62, 327
MalI GATC 5 cut(s) 11, 122, 425, 443, 456
MboI GATC 5 cut(s) 9, 120, 423, 441, 454
MboII GAAGA 3 cut(s) 145, 298, 462
MfeI CAATTG 1 cut(s) 114
MluCI AATT 3 cut(s) 114, 246, 283
MnlI CCTC 5 cut(s) 56, 98, 112, 129, 314
MseI TTAA 1 cut(s) 353
MspI CCGG 1 cut(s) 581
MspR9I CCNGG 2 cut(s) 581, 636
MunI CAATTG 1 cut(s) 114
MvaI CCWGG 1 cut(s) 636
MwoI GCNNNNNNNGC 3 cut(s) 242, 396, 622
NciI CCSGG 1 cut(s) 581
NcoI CCATGG 1 cut(s) 405
NdeII GATC 5 cut(s) 9, 120, 423, 441, 454
NlaIII CATG 4 cut(s) 43, 71, 187, 409
NlaIV GGNNCC 3 cut(s) 36, 149, 364
NmuCI GTSAC 1 cut(s) 327
NspI RCATGY 1 cut(s) 187
PkrI GCNGC 3 cut(s) 271, 370, 401
Psp1406I AACGTT 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 634
PspEI GGTNACC 1 cut(s) 62
PspGI CCWGG 1 cut(s) 634
PspN4I GGNNCC 3 cut(s) 36, 149, 364
PspPI GGNCC 1 cut(s) 572
RsaI GTAC 1 cut(s) 109
RsaNI GTAC 1 cut(s) 108
SaqAI TTAA 1 cut(s) 353
SatI GCNGC 3 cut(s) 270, 369, 400
Sau3AI GATC 5 cut(s) 9, 120, 423, 441, 454
Sau96I GGNCC 1 cut(s) 572
ScrFI CCNGG 2 cut(s) 581, 636
SfaNI GCATC 4 cut(s) 418, 522, 549, 555
SfcI CTRYAG 1 cut(s) 289
SinI GGWCC 1 cut(s) 572
SmlI CTYRAG 3 cut(s) 89, 202, 494
SmoI CTYRAG 3 cut(s) 89, 202, 494
Sse9I AATT 3 cut(s) 114, 246, 283
SsiI CCGC 1 cut(s) 366
SspMI CTAG 3 cut(s) 20, 152, 158
StyD4I CCNGG 2 cut(s) 579, 634
StyI CCWWGG 1 cut(s) 405
TaaI ACNGT 2 cut(s) 58, 241
TaiI ACGT 1 cut(s) 30
TaqI TCGA 1 cut(s) 426
TasI AATT 3 cut(s) 114, 246, 283
Tru1I TTAA 1 cut(s) 353
Tru9I TTAA 1 cut(s) 353
TseFI GTSAC 1 cut(s) 327
TseI GCWGC 3 cut(s) 269, 368, 399
Tsp45I GTSAC 1 cut(s) 327
VpaK11BI GGWCC 1 cut(s) 572
XbaI TCTAGA 1 cut(s) 19
XceI RCATGY 1 cut(s) 187
XspI CTAG 3 cut(s) 20, 152, 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.