RLG00000025016

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0004770
Physical Location & Seq
Forward (+)
41330829 .. 41331850
1022 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025016

Sequence Viewer

Length: 675 bp
ATGGGAACTACTTTTACTTTAGACACCATTCTAGTTCCCCTAAGCCTCTTCGTTCTAATAGCTTACCATGCATATTTATGGTGTATCTTCAAGACCAAACCCTCTCACACGACCATCGGAATTGAGTCAGTCAGAAGAAGAATATGGTTTCTAGAGATTATGCAGGGTGATGGTAGGAAGTCTATGTTGGCAGTCCAAAACTTGAGAAACACTCAAATGGTGGCAATAATCACAGCTTCAACAGCAATTGCCTTCAGCTTGGCTTTGGCAGCTCTGACGAACAACGCTTACAATGCAAGCCACCTCTTTGTTAAAACAGCATTTTTCGGCTTGCAGTCGGGGAGGATCTTTGCCCTAAAGTATGGCTTGGCCTATTTCGTCTTGCTGTTCAGCTCATTATGCAGCTCCATGGCCACTGGATTTATGATCGATGCCATTTTTCTACTAAATACTTCTGCCGAGTTCTCATCTTCAGGAATAACACTACTCCTATTTGAACGAGGTTACATGTTGGCTCAAATTGGCGAAAGGTTGCTTTGTATCTCCTTTCCTTTGTTGTTGTGGTTGTTTGGTCCAGTGCCAGTGGCCTTGTCATCCCTGGCCTTGGTTTGGTGGTTTAAAGAGCTTGATTTTGTTGGCAAATTCACCCAAAGTAACAGGCAAAGTCTCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

225

Amino Acids

24.97

Weight (kDa)

9.44

Isoelectric Point (pI)

35.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 10 - 212 3.5e-44 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 353
AcoI YGGCCR 1 cut(s) 411
AcsI RAATTY 1 cut(s) 641
AcuI CTGAAG 2 cut(s) 238, 456
AfiI CCNNNNNNNGG 2 cut(s) 604, 609
AflIII ACRYGT 1 cut(s) 507
AgsI TTSAA 3 cut(s) 91, 240, 497
AjnI CCWGG 1 cut(s) 597
AjuI GAANNNNNNNTTGG 2 cut(s) 170, 202
AluBI AGCT 8 cut(s) 62, 236, 258, 272, 393, 405, 625, 672
AluI AGCT 8 cut(s) 62, 236, 258, 272, 393, 405, 625, 672
Alw26I GTCTC 1 cut(s) 671
AlwI GGATC 1 cut(s) 353
AoxI GGCC 4 cut(s) 369, 411, 585, 600
ApeKI GCWGC 2 cut(s) 269, 402
ApoI RAATTY 1 cut(s) 641
AspS9I GGNCC 1 cut(s) 572
AsuHPI GGTGA 2 cut(s) 179, 637
AvaII GGWCC 1 cut(s) 572
BalI TGGCCA 1 cut(s) 413
BbvI GCAGC 2 cut(s) 281, 414
BccI CCATC 2 cut(s) 122, 164
BciT130I CCWGG 1 cut(s) 599
BcoDI GTCTC 1 cut(s) 671
BfaI CTAG 2 cut(s) 32, 152
BisI GCNGC 2 cut(s) 270, 403
BlsI GCNGC 2 cut(s) 271, 404
Bme1390I CCNGG 1 cut(s) 599
Bme18I GGWCC 1 cut(s) 572
BmgT120I GGNCC 1 cut(s) 572
BmrFI CCNGG 1 cut(s) 599
BmsI GCATC 1 cut(s) 421
BplI GAGNNNNNCTC 2 cut(s) 196, 228
Bpu10I CCTNAGC 1 cut(s) 41
BpuEI CTTGAG 1 cut(s) 223
Bsa29I ATCGAT 1 cut(s) 429
BsaJI CCNNGG 3 cut(s) 408, 597, 603
Bsc4I CCNNNNNNNGG 2 cut(s) 604, 609
Bse1I ACTGG 3 cut(s) 421, 575, 581
BseBI CCWGG 1 cut(s) 599
BseCI ATCGAT 1 cut(s) 429
BseDI CCNNGG 3 cut(s) 408, 597, 603
BseGI GGATG 1 cut(s) 593
BseLI CCNNNNNNNGG 2 cut(s) 604, 609
BseNI ACTGG 3 cut(s) 421, 575, 581
BseXI GCAGC 2 cut(s) 281, 414
BshFI GGCC 4 cut(s) 371, 413, 587, 602
BshVI ATCGAT 1 cut(s) 429
BslI CCNNNNNNNGG 2 cut(s) 604, 609
BsmAI GTCTC 1 cut(s) 671
BsnI GGCC 4 cut(s) 371, 413, 587, 602
Bsp143I GATC 2 cut(s) 345, 426
Bsp19I CCATGG 1 cut(s) 408
BspANI GGCC 4 cut(s) 371, 413, 587, 602
BspDI ATCGAT 1 cut(s) 429
BspPI GGATC 1 cut(s) 353
BsrI ACTGG 3 cut(s) 421, 575, 581
BssECI CCNNGG 3 cut(s) 408, 597, 603
BssMI GATC 2 cut(s) 345, 426
BssT1I CCWWGG 2 cut(s) 408, 603
Bst2UI CCWGG 1 cut(s) 599
Bst6I CTCTTC 1 cut(s) 53
BstC8I GCNNGC 2 cut(s) 298, 332
BstDEI CTNAG 2 cut(s) 41, 668
BstDSI CCRYGG 1 cut(s) 408
BstF5I GGATG 1 cut(s) 593
BstKTI GATC 2 cut(s) 348, 429
BstMAI GTCTC 1 cut(s) 671
BstMBI GATC 2 cut(s) 345, 426
BstMWI GCNNNNNNNGC 5 cut(s) 68, 242, 269, 293, 399
BstNI CCWGG 1 cut(s) 599
BstNSI RCATGY 1 cut(s) 511
BstSCI CCNGG 1 cut(s) 597
BstV1I GCAGC 2 cut(s) 281, 414
BstX2I RGATCY 1 cut(s) 345
BstYI RGATCY 1 cut(s) 345
Bsu15I ATCGAT 1 cut(s) 429
BsuRI GGCC 4 cut(s) 371, 413, 587, 602
BsuTUI ATCGAT 1 cut(s) 429
BtgI CCRYGG 1 cut(s) 408
BtsCI GGATG 1 cut(s) 593
BtsIMutI CAGTG 3 cut(s) 414, 582, 588
Cac8I GCNNGC 2 cut(s) 298, 332
Cfr13I GGNCC 1 cut(s) 572
ClaI ATCGAT 1 cut(s) 429
CviAII CATG 3 cut(s) 68, 409, 508
DdeI CTNAG 2 cut(s) 41, 668
DpnI GATC 2 cut(s) 347, 428
DpnII GATC 2 cut(s) 345, 426
DraI TTTAAA 1 cut(s) 619
EaeI YGGCCR 1 cut(s) 411
Eam1104I CTCTTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 53
Eco130I CCWWGG 2 cut(s) 408, 603
Eco47I GGWCC 1 cut(s) 572
Eco57I CTGAAG 2 cut(s) 238, 456
EcoRII CCWGG 1 cut(s) 597
EcoT14I CCWWGG 2 cut(s) 408, 603
EcoT22I ATGCAT 1 cut(s) 73
ErhI CCWWGG 2 cut(s) 408, 603
FaeI CATG 3 cut(s) 71, 412, 511
FalI AAGNNNNNCTT 2 cut(s) 350, 382
FatI CATG 3 cut(s) 67, 408, 507
Fnu4HI GCNGC 2 cut(s) 270, 403
FokI GGATG 1 cut(s) 580
Fsp4HI GCNGC 2 cut(s) 270, 403
FspBI CTAG 2 cut(s) 32, 152
GluI GCNGC 2 cut(s) 270, 403
HaeIII GGCC 4 cut(s) 371, 413, 587, 602
Hin1II CATG 3 cut(s) 71, 412, 511
HinfI GANTC 1 cut(s) 125
HphI GGTGA 2 cut(s) 179, 637
Hpy188I TCNGA 3 cut(s) 119, 134, 276
Hpy188III TCNNGA 3 cut(s) 91, 152, 474
HpyAV CCTTC 1 cut(s) 262
HpyCH4V TGCA 5 cut(s) 71, 163, 296, 334, 402
HpyF10VI GCNNNNNNNGC 5 cut(s) 68, 242, 269, 293, 399
HpyF3I CTNAG 2 cut(s) 41, 668
Hsp92II CATG 3 cut(s) 71, 412, 511
Kzo9I GATC 2 cut(s) 345, 426
LmnI GCTCC 1 cut(s) 410
LpnPI CCDG 8 cut(s) 149, 402, 459, 584, 588, 594, 611, 643
Lsp1109I GCAGC 2 cut(s) 281, 414
LweI GCATC 1 cut(s) 421
MaeI CTAG 2 cut(s) 32, 152
MaeIII GTNAC 2 cut(s) 503, 653
MalI GATC 2 cut(s) 347, 428
MboI GATC 2 cut(s) 345, 426
MboII GAAGA 5 cut(s) 40, 79, 147, 150, 462
MfeI CAATTG 1 cut(s) 246
MflI RGATCY 1 cut(s) 345
MlsI TGGCCA 1 cut(s) 413
MluCI AATT 4 cut(s) 120, 246, 519, 641
MluNI TGGCCA 1 cut(s) 413
MlyI GAGTC 1 cut(s) 134
MnlI CCTC 5 cut(s) 56, 112, 314, 336, 494
Mox20I TGGCCA 1 cut(s) 413
Mph1103I ATGCAT 1 cut(s) 73
MscI TGGCCA 1 cut(s) 413
MseI TTAA 2 cut(s) 312, 618
MslI CAYNNNNRTG 2 cut(s) 76, 215
Msp20I TGGCCA 1 cut(s) 413
MspA1I CMGCKG 1 cut(s) 672
MspR9I CCNGG 1 cut(s) 599
MunI CAATTG 1 cut(s) 246
MvaI CCWGG 1 cut(s) 599
MwoI GCNNNNNNNGC 5 cut(s) 68, 242, 269, 293, 399
NcoI CCATGG 1 cut(s) 408
NdeII GATC 2 cut(s) 345, 426
NlaIII CATG 3 cut(s) 71, 412, 511
NmeAIII GCCGAG 1 cut(s) 484
NsiI ATGCAT 1 cut(s) 73
NspI RCATGY 1 cut(s) 511
PciI ACATGT 1 cut(s) 507
PkrI GCNGC 2 cut(s) 271, 404
PleI GAGTC 1 cut(s) 133
PpsI GAGTC 1 cut(s) 133
PscI ACATGT 1 cut(s) 507
Psp6I CCWGG 1 cut(s) 597
PspGI CCWGG 1 cut(s) 597
PspPI GGNCC 1 cut(s) 572
PsrI GAACNNNNNNTAC 3 cut(s) 30, 272, 304
PsuI RGATCY 1 cut(s) 345
PvuII CAGCTG 1 cut(s) 672
RseI CAYNNNNRTG 2 cut(s) 76, 215
SaqAI TTAA 2 cut(s) 312, 618
SatI GCNGC 2 cut(s) 270, 403
Sau3AI GATC 2 cut(s) 345, 426
Sau96I GGNCC 1 cut(s) 572
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 1 cut(s) 599
SfaNI GCATC 1 cut(s) 421
SinI GGWCC 1 cut(s) 572
SmiMI CAYNNNNRTG 2 cut(s) 76, 215
SmlI CTYRAG 1 cut(s) 202
SmoI CTYRAG 1 cut(s) 202
Sse9I AATT 4 cut(s) 120, 246, 519, 641
SspMI CTAG 2 cut(s) 32, 152
StyD4I CCNGG 1 cut(s) 597
StyI CCWWGG 2 cut(s) 408, 603
TaqI TCGA 1 cut(s) 429
TasI AATT 4 cut(s) 120, 246, 519, 641
Tru1I TTAA 2 cut(s) 312, 618
Tru9I TTAA 2 cut(s) 312, 618
TscAI CASTG 3 cut(s) 421, 582, 588
TseI GCWGC 2 cut(s) 269, 402
TspRI CASTG 3 cut(s) 421, 582, 588
VpaK11BI GGWCC 1 cut(s) 572
XapI RAATTY 1 cut(s) 641
XbaI TCTAGA 1 cut(s) 151
XceI RCATGY 1 cut(s) 511
XspI CTAG 2 cut(s) 32, 152
Zsp2I ATGCAT 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.