Rorug03G0042400

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0004770
Physical Location & Seq
Reverse (-)
3287374 .. 3288554
1181 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0042400.1

Sequence Viewer

Length: 981 bp
ATGACTGCAAAGGATCTTCCAGTTGGCTTTAAGTTTCATCCAACTGACCAAGAATTGTTAGGATATTACCTTCGCAGCAAGGTTTCTGGAGAACCATTCAGGTACGAGCCGCGAATCAAAAGCTTTGATCTTTATGGCAAGACAGAGCCATGGGACATATGGACAGAGTTTGGAGGACCGGAACTAGACCAAAGTGAAGACCTCTATTTCTTTACCAAACTGAAGAAGATGGGCGACTCGGCATCTATGTATCGTGCTCGAAGGTCGGGTTCTGGGACCTGGAAAGGTAAGAACAAAGGGGTCACCGTTTATGATTCCAAGAATAAGAAGATTGTGTTAGGGACACGCAAACTGTTTCATTATGAGAATAATGAATCGGATCAAAACCGTTGTTGGATCATGCATGAGTATAGCATTCCTCCTTCAAACACTAGCGATCTTCCTCATGAATATGTGCTCTGCCGAATCCGAATCAATGTGGATAGAAGGAGAAAGTTGGTTGCAAAGAAAGTGTCCCAAAACCATGTTAATAATGAGGCCATTGATCATGAGTATGAACTGGAGGATCAGACCACTATGACTCAGGAAACCTATGCCGAGCTGAATGACATTAATCCACCATTAGAGGACAATGATGCCTTCAACACCAGCACTGATCATGTTAGCAATGCAAGCAAATCGCTTCACACATCTCAACCACTGCCATCATATCATCCTAAATTAGAGGTTATGGCATGTGAAACAAATGTGGAGCTTACTGATCATGACAATGTTCACGACTATCCGCTTTATGAGGATTATGTGTTCAACTCGATCAATGTTGGTGATACTCATCGACTGCAGCACCAGTCTTTCAGAACACTGACCGAGACACTAAAGAGTGGATCATACCCTCCATATTCTTATCCCAGCTTCCAAAGGTCCAGTAACAAAGTCGGAGAGAGCAGTGCTGGTGCTGTGGCTAAGGCCGGGCAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

326

Amino Acids

37.52

Weight (kDa)

6.02

Isoelectric Point (pI)

44.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 7 - 139 8.3e-28 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 112
AciI CCGC 2 cut(s) 110, 785
AclWI GGATC 5 cut(s) 21, 387, 404, 573, 892
AcuI CTGAAG 1 cut(s) 242
AfaI GTAC 1 cut(s) 104
AgsI TTSAA 3 cut(s) 426, 643, 808
AjnI CCWGG 1 cut(s) 278
AloI GAACNNNNNNTCC 2 cut(s) 788, 820
AluBI AGCT 4 cut(s) 123, 601, 754, 912
AluI AGCT 4 cut(s) 123, 601, 754, 912
Alw21I GWGCWC 2 cut(s) 259, 459
Alw26I GTCTC 1 cut(s) 863
AlwI GGATC 5 cut(s) 21, 387, 404, 573, 892
AoxI GGCC 2 cut(s) 537, 966
ApeKI GCWGC 2 cut(s) 75, 841
ArsI GACNNNNNNTTYG 2 cut(s) 497, 529
AseI ATTAAT 1 cut(s) 612
AspS9I GGNCC 3 cut(s) 176, 276, 921
AsuC2I CCSGG 1 cut(s) 970
AsuHPI GGTGA 2 cut(s) 295, 836
AvaII GGWCC 3 cut(s) 176, 276, 921
BbsI GAAGAC 1 cut(s) 204
Bbv12I GWGCWC 2 cut(s) 259, 459
BbvI GCAGC 2 cut(s) 87, 853
BccI CCATC 2 cut(s) 223, 712
BcgI CGANNNNNNTGC 2 cut(s) 660, 694
BciT130I CCWGG 1 cut(s) 280
BclI TGATCA 3 cut(s) 544, 655, 760
BcnI CCSGG 1 cut(s) 970
BcoDI GTCTC 1 cut(s) 863
BfaI CTAG 2 cut(s) 185, 432
BfmI CTRYAG 1 cut(s) 839
BisI GCNGC 3 cut(s) 76, 110, 842
BlsI GCNGC 3 cut(s) 77, 111, 843
Bme1390I CCNGG 2 cut(s) 280, 970
Bme18I GGWCC 3 cut(s) 176, 276, 921
BmgT120I GGNCC 3 cut(s) 176, 276, 921
BmiI GGNNCC 1 cut(s) 277
BmrFI CCNGG 2 cut(s) 280, 970
BmsI GCATC 2 cut(s) 251, 625
BpiI GAAGAC 1 cut(s) 204
BpmI CTGGAG 2 cut(s) 108, 581
Bpu10I CCTNAGC 1 cut(s) 963
BpuMI CCSGG 1 cut(s) 970
BsaBI GATNNNNATC 1 cut(s) 831
BsaJI CCNNGG 1 cut(s) 149
BsaWI WCCGGW 1 cut(s) 178
Bse1I ACTGG 4 cut(s) 20, 564, 847, 924
Bse3DI GCAATG 1 cut(s) 673
Bse8I GATNNNNATC 1 cut(s) 831
BseBI CCWGG 1 cut(s) 280
BseDI CCNNGG 1 cut(s) 149
BseGI GGATG 2 cut(s) 37, 712
BseJI GATNNNNATC 1 cut(s) 831
BseMI GCAATG 1 cut(s) 673
BseMII CTCAG 1 cut(s) 596
BseNI ACTGG 4 cut(s) 20, 564, 847, 924
BseXI GCAGC 2 cut(s) 87, 853
BseYI CCCAGC 1 cut(s) 908
Bsh1236I CGCG 1 cut(s) 112
BshFI GGCC 2 cut(s) 539, 968
BsiHKAI GWGCWC 2 cut(s) 259, 459
BsiSI CCGG 2 cut(s) 179, 969
BslFI GGGAC 4 cut(s) 167, 289, 355, 499
BsmAI GTCTC 1 cut(s) 863
BsmFI GGGAC 4 cut(s) 167, 289, 355, 499
BsmI GAATGC 1 cut(s) 414
BsnI GGCC 2 cut(s) 539, 968
Bsp1286I GDGCHC 2 cut(s) 259, 459
Bsp19I CCATGG 1 cut(s) 149
BspACI CCGC 2 cut(s) 110, 785
BspANI GGCC 2 cut(s) 539, 968
BspCNI CTCAG 1 cut(s) 595
BspFNI CGCG 1 cut(s) 112
BspHI TCATGA 3 cut(s) 445, 547, 763
BspLI GGNNCC 1 cut(s) 277
BspMAI CTGCAG 1 cut(s) 843
BspPI GGATC 5 cut(s) 21, 387, 404, 573, 892
BsrDI GCAATG 1 cut(s) 673
BsrI ACTGG 4 cut(s) 20, 564, 847, 924
BssECI CCNNGG 1 cut(s) 149
BssT1I CCWWGG 1 cut(s) 149
Bst2UI CCWGG 1 cut(s) 280
Bst4CI ACNGT 3 cut(s) 307, 354, 389
BstC8I GCNNGC 1 cut(s) 673
BstDEI CTNAG 2 cut(s) 582, 963
BstDSI CCRYGG 1 cut(s) 149
BstEII GGTNACC 1 cut(s) 301
BstF5I GGATG 2 cut(s) 37, 712
BstFNI CGCG 1 cut(s) 112
BstMAI GTCTC 1 cut(s) 863
BstMWI GCNNNNNNNGC 1 cut(s) 672
BstNI CCWGG 1 cut(s) 280
BstNSI RCATGY 1 cut(s) 738
BstPI GGTNACC 1 cut(s) 301
BstSCI CCNGG 2 cut(s) 278, 968
BstSFI CTRYAG 1 cut(s) 839
BstUI CGCG 1 cut(s) 112
BstV1I GCAGC 2 cut(s) 87, 853
BstV2I GAAGAC 1 cut(s) 204
BstX2I RGATCY 1 cut(s) 13
BstYI RGATCY 1 cut(s) 13
BsuRI GGCC 2 cut(s) 539, 968
BtgI CCRYGG 1 cut(s) 149
BtsCI GGATG 2 cut(s) 37, 712
BtsI GCAGTG 2 cut(s) 698, 952
BtsIMutI CAGTG 4 cut(s) 651, 698, 860, 952
Cac8I GCNNGC 1 cut(s) 673
CciI TCATGA 3 cut(s) 445, 547, 763
Cfr13I GGNCC 3 cut(s) 176, 276, 921
Csp6I GTAC 1 cut(s) 103
CviAII CATG 9 cut(s) 150, 400, 404, 446, 524, 548, 659, 735, 764
CviQI GTAC 1 cut(s) 103
DdeI CTNAG 2 cut(s) 582, 963
Eco130I CCWWGG 1 cut(s) 149
Eco47I GGWCC 3 cut(s) 176, 276, 921
Eco57I CTGAAG 1 cut(s) 242
Eco91I GGTNACC 1 cut(s) 301
EcoO109I RGGNCCY 1 cut(s) 276
EcoO65I GGTNACC 1 cut(s) 301
EcoRII CCWGG 1 cut(s) 278
EcoT14I CCWWGG 1 cut(s) 149
EcoT22I ATGCAT 1 cut(s) 405
ErhI CCWWGG 1 cut(s) 149
FaeI CATG 9 cut(s) 153, 403, 407, 449, 527, 551, 662, 738, 767
FaqI GGGAC 4 cut(s) 167, 289, 355, 499
FatI CATG 9 cut(s) 149, 399, 403, 445, 523, 547, 658, 734, 763
FauNDI CATATG 1 cut(s) 158
FbaI TGATCA 3 cut(s) 544, 655, 760
Fnu4HI GCNGC 3 cut(s) 76, 110, 842
FokI GGATG 2 cut(s) 24, 699
Fsp4HI GCNGC 3 cut(s) 76, 110, 842
FspBI CTAG 2 cut(s) 185, 432
GluI GCNGC 3 cut(s) 76, 110, 842
GsaI CCCAGC 1 cut(s) 912
GsuI CTGGAG 2 cut(s) 108, 581
HaeIII GGCC 2 cut(s) 539, 968
HapII CCGG 2 cut(s) 179, 969
Hin1II CATG 9 cut(s) 153, 403, 407, 449, 527, 551, 662, 738, 767
HindIII AAGCTT 1 cut(s) 121
HinfI GANTC 7 cut(s) 114, 236, 314, 374, 465, 471, 580
HpaII CCGG 2 cut(s) 179, 969
HphI GGTGA 2 cut(s) 295, 836
Hpy166II GTNNAC 1 cut(s) 775
Hpy188I TCNGA 5 cut(s) 379, 470, 570, 857, 938
Hpy188III TCNNGA 6 cut(s) 87, 446, 548, 584, 764, 776
Hpy8I GTNNAC 1 cut(s) 775
HpyAV CCTTC 5 cut(s) 80, 255, 432, 480, 649
HpyCH4III ACNGT 3 cut(s) 307, 354, 389
HpyCH4V TGCA 5 cut(s) 8, 403, 503, 671, 841
HpyF10VI GCNNNNNNNGC 1 cut(s) 672
HpyF3I CTNAG 2 cut(s) 582, 963
Hsp92II CATG 9 cut(s) 153, 403, 407, 449, 527, 551, 662, 738, 767
Ksp22I TGATCA 3 cut(s) 544, 655, 760
LmnI GCTCC 1 cut(s) 751
Lsp1109I GCAGC 2 cut(s) 87, 853
LweI GCATC 2 cut(s) 251, 625
MaeI CTAG 2 cut(s) 185, 432
MaeIII GTNAC 2 cut(s) 301, 926
MboII GAAGA 6 cut(s) 8, 209, 235, 238, 340, 431
MflI RGATCY 1 cut(s) 13
MhlI GDGCHC 2 cut(s) 259, 459
MluCI AATT 2 cut(s) 53, 719
MlyI GAGTC 2 cut(s) 230, 574
MmeI TCCRAC 3 cut(s) 65, 374, 916
Mph1103I ATGCAT 1 cut(s) 405
MseI TTAA 3 cut(s) 30, 528, 612
MslI CAYNNNNRTG 3 cut(s) 450, 552, 768
MspI CCGG 2 cut(s) 179, 969
MspR9I CCNGG 2 cut(s) 280, 970
Mva1269I GAATGC 1 cut(s) 414
MvaI CCWGG 1 cut(s) 280
MvnI CGCG 1 cut(s) 112
MwoI GCNNNNNNNGC 1 cut(s) 672
NciI CCSGG 1 cut(s) 970
NcoI CCATGG 1 cut(s) 149
NdeI CATATG 1 cut(s) 158
NlaIII CATG 9 cut(s) 153, 403, 407, 449, 527, 551, 662, 738, 767
NlaIV GGNNCC 1 cut(s) 277
NmeAIII GCCGAG 2 cut(s) 218, 622
NmuCI GTSAC 1 cut(s) 301
NsiI ATGCAT 1 cut(s) 405
NspI RCATGY 1 cut(s) 738
PagI TCATGA 3 cut(s) 445, 547, 763
PctI GAATGC 1 cut(s) 414
PfeI GAWTC 5 cut(s) 114, 314, 374, 465, 471
PkrI GCNGC 3 cut(s) 77, 111, 843
PleI GAGTC 2 cut(s) 230, 574
PpsI GAGTC 2 cut(s) 230, 574
PpuMI RGGWCCY 1 cut(s) 276
PshBI ATTAAT 1 cut(s) 612
Psp5II RGGWCCY 1 cut(s) 276
Psp6I CCWGG 1 cut(s) 278
PspEI GGTNACC 1 cut(s) 301
PspFI CCCAGC 1 cut(s) 908
PspGI CCWGG 1 cut(s) 278
PspN4I GGNNCC 1 cut(s) 277
PspPI GGNCC 3 cut(s) 176, 276, 921
PspPPI RGGWCCY 1 cut(s) 276
PstI CTGCAG 1 cut(s) 843
PsuI RGATCY 1 cut(s) 13
RsaI GTAC 1 cut(s) 104
RsaNI GTAC 1 cut(s) 103
RseI CAYNNNNRTG 3 cut(s) 450, 552, 768
SaqAI TTAA 3 cut(s) 30, 528, 612
SatI GCNGC 3 cut(s) 76, 110, 842
Sau96I GGNCC 3 cut(s) 176, 276, 921
SchI GAGTC 2 cut(s) 230, 574
ScrFI CCNGG 2 cut(s) 280, 970
SduI GDGCHC 2 cut(s) 259, 459
SfaNI GCATC 2 cut(s) 251, 625
SfcI CTRYAG 1 cut(s) 839
SinI GGWCC 3 cut(s) 176, 276, 921
SmiMI CAYNNNNRTG 3 cut(s) 450, 552, 768
Sse9I AATT 2 cut(s) 53, 719
SsiI CCGC 2 cut(s) 110, 785
SspMI CTAG 2 cut(s) 185, 432
StyD4I CCNGG 2 cut(s) 278, 968
StyI CCWWGG 1 cut(s) 149
TaaI ACNGT 3 cut(s) 307, 354, 389
TaqI TCGA 3 cut(s) 259, 812, 835
TaqII GACCGA 1 cut(s) 881
TasI AATT 2 cut(s) 53, 719
TauI GCSGC 1 cut(s) 112
TfiI GAWTC 5 cut(s) 114, 314, 374, 465, 471
Tru1I TTAA 3 cut(s) 30, 528, 612
Tru9I TTAA 3 cut(s) 30, 528, 612
TscAI CASTG 4 cut(s) 658, 705, 867, 952
TseFI GTSAC 1 cut(s) 301
TseI GCWGC 2 cut(s) 75, 841
Tsp45I GTSAC 1 cut(s) 301
TspDTI ATGAA 5 cut(s) 26, 347, 387, 462, 570
TspRI CASTG 4 cut(s) 658, 705, 867, 952
VpaK11BI GGWCC 3 cut(s) 176, 276, 921
VspI ATTAAT 1 cut(s) 612
XceI RCATGY 1 cut(s) 738
XcmI CCANNNNNNNNNTGG 1 cut(s) 156
XspI CTAG 2 cut(s) 185, 432
Zsp2I ATGCAT 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.