pycom04g15470

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0004770
Physical Location & Seq
Forward (+)
18346599 .. 18347634
1036 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g15470.1

Sequence Viewer

Length: 678 bp
ATGGGAGTGATCACTTATCTAGACACCGTTTTGGTCCCCATGAGCCTCTTTCTCACAGTTGGTTACCATGCCTATCTATGGCACAACCTCAAGAACAAGCCCTCTCGTACCACAATTGGGATCAATATGCTGAAGAGGAGAGCTTGGTTCCTAGAGCTAGATGGGGGTGATGATAAGAAAGGCATGTTAGCAGTCCAAAGCTTGAGAAATACTCTTATGGGGACAACACTCACAGCCACAGTAGCAATTCTTATTCAAGTCTCGCTGGCAGCTCTAATAAACAATTCCTATAGTGCAACCAACCTCTTCAGCAATACCTTTTTTGGGTCACAATCCACCAAGATTTTTGCCTTAAAATATGGCTCCGCAGCTTTGTCTTTGTCAGTTTGCTTCTTGTGCAGCTCCATGGCGATTGCGTTTTTGATCGATGCCAACTTCTTGATCAATGCTTGTGGTGATCAGGAGTTCTCATCTTCTGGACACGCAAGAACCATACTTGAGAAAGGGTTTATTTTGGCTCTTGTAAGCAACAGGATGCTGTGCATCACTTTTCCTATGCTGTTGTGGATGCTTGGTCCTGTCCCGGTTTGGTTGTCATCTTTGGCGATGGTTTGGGGGCTTTATGGGCTGGATTTTCCTGGCAAGTTCAATAAAAGCAATAAGCAAAGTCTCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

226

Amino Acids

24.7

Weight (kDa)

9.36

Isoelectric Point (pI)

42.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 10 - 212 3e-52 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 366
AclWI GGATC 1 cut(s) 128
AcuI CTGAAG 2 cut(s) 152, 292
AfaI GTAC 1 cut(s) 109
AfiI CCNNNNNNNGG 3 cut(s) 78, 117, 324
AgsI TTSAA 2 cut(s) 257, 649
AjnI CCWGG 1 cut(s) 637
AluBI AGCT 6 cut(s) 143, 157, 201, 272, 371, 402
AluI AGCT 6 cut(s) 143, 157, 201, 272, 371, 402
Alw26I GTCTC 2 cut(s) 265, 674
AlwI GGATC 1 cut(s) 128
ApeKI GCWGC 3 cut(s) 269, 368, 399
AspS9I GGNCC 2 cut(s) 34, 575
AsuC2I CCSGG 1 cut(s) 584
AsuHPI GGTGA 2 cut(s) 179, 467
AvaII GGWCC 2 cut(s) 34, 575
BbvI GCAGC 3 cut(s) 281, 380, 411
BccI CCATC 2 cut(s) 155, 601
BciT130I CCWGG 1 cut(s) 639
BclI TGATCA 3 cut(s) 9, 441, 457
BcnI CCSGG 1 cut(s) 584
BcoDI GTCTC 2 cut(s) 265, 674
BfaI CTAG 3 cut(s) 20, 152, 158
BfmI CTRYAG 1 cut(s) 289
BisI GCNGC 3 cut(s) 270, 369, 400
BlsI GCNGC 3 cut(s) 271, 370, 401
Bme1390I CCNGG 2 cut(s) 584, 639
Bme18I GGWCC 2 cut(s) 34, 575
BmgT120I GGNCC 2 cut(s) 34, 575
BmiI GGNNCC 3 cut(s) 36, 149, 364
BmrFI CCNGG 2 cut(s) 584, 639
BmsI GCATC 4 cut(s) 418, 525, 552, 558
BplI GAGNNNNNCTC 2 cut(s) 196, 228
BpuEI CTTGAG 3 cut(s) 74, 223, 518
BpuMI CCSGG 1 cut(s) 584
Bsa29I ATCGAT 1 cut(s) 426
BsaJI CCNNGG 1 cut(s) 405
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 117, 324
BseBI CCWGG 1 cut(s) 639
BseCI ATCGAT 1 cut(s) 426
BseDI CCNNGG 1 cut(s) 405
BseGI GGATG 2 cut(s) 540, 573
BseLI CCNNNNNNNGG 3 cut(s) 78, 117, 324
BseRI GAGGAG 1 cut(s) 151
BseXI GCAGC 3 cut(s) 281, 380, 411
BsgI GTGCAG 1 cut(s) 418
BshVI ATCGAT 1 cut(s) 426
BsiSI CCGG 1 cut(s) 584
BslFI GGGAC 3 cut(s) 20, 235, 566
BslI CCNNNNNNNGG 3 cut(s) 78, 117, 324
BsmAI GTCTC 2 cut(s) 265, 674
BsmFI GGGAC 3 cut(s) 20, 235, 566
Bsp143I GATC 5 cut(s) 9, 120, 423, 441, 457
Bsp19I CCATGG 1 cut(s) 405
BspACI CCGC 1 cut(s) 366
BspDI ATCGAT 1 cut(s) 426
BspLI GGNNCC 3 cut(s) 36, 149, 364
BspPI GGATC 1 cut(s) 128
BssECI CCNNGG 1 cut(s) 405
BssMI GATC 5 cut(s) 9, 120, 423, 441, 457
BssT1I CCWWGG 1 cut(s) 405
Bst2UI CCWGG 1 cut(s) 639
Bst4CI ACNGT 3 cut(s) 28, 58, 241
Bst6I CTCTTC 2 cut(s) 128, 311
BstC8I GCNNGC 1 cut(s) 267
BstDEI CTNAG 1 cut(s) 671
BstDSI CCRYGG 1 cut(s) 405
BstEII GGTNACC 1 cut(s) 62
BstF5I GGATG 2 cut(s) 540, 573
BstKTI GATC 5 cut(s) 12, 123, 426, 444, 460
BstMAI GTCTC 2 cut(s) 265, 674
BstMBI GATC 5 cut(s) 9, 120, 423, 441, 457
BstMWI GCNNNNNNNGC 3 cut(s) 242, 396, 625
BstNI CCWGG 1 cut(s) 639
BstNSI RCATGY 1 cut(s) 187
BstPI GGTNACC 1 cut(s) 62
BstSCI CCNGG 2 cut(s) 582, 637
BstSFI CTRYAG 1 cut(s) 289
BstV1I GCAGC 3 cut(s) 281, 380, 411
Bsu15I ATCGAT 1 cut(s) 426
BsuTUI ATCGAT 1 cut(s) 426
BtgI CCRYGG 1 cut(s) 405
BtgZI GCGATG 1 cut(s) 620
BtsCI GGATG 2 cut(s) 540, 573
Cac8I GCNNGC 1 cut(s) 267
Cfr13I GGNCC 2 cut(s) 34, 575
ClaI ATCGAT 1 cut(s) 426
Csp6I GTAC 1 cut(s) 108
CspCI CAANNNNNGTGG 2 cut(s) 433, 468
CviAII CATG 4 cut(s) 40, 68, 184, 406
CviQI GTAC 1 cut(s) 108
DdeI CTNAG 1 cut(s) 671
DpnI GATC 5 cut(s) 11, 122, 425, 443, 459
DpnII GATC 5 cut(s) 9, 120, 423, 441, 457
Eam1104I CTCTTC 2 cut(s) 128, 311
EarI CTCTTC 2 cut(s) 128, 311
Eco130I CCWWGG 1 cut(s) 405
Eco47I GGWCC 2 cut(s) 34, 575
Eco57I CTGAAG 2 cut(s) 152, 292
Eco91I GGTNACC 1 cut(s) 62
EcoO65I GGTNACC 1 cut(s) 62
EcoRII CCWGG 1 cut(s) 637
EcoT14I CCWWGG 1 cut(s) 405
ErhI CCWWGG 1 cut(s) 405
FaeI CATG 4 cut(s) 43, 71, 187, 409
FaqI GGGAC 3 cut(s) 20, 235, 566
FatI CATG 4 cut(s) 39, 67, 183, 405
FbaI TGATCA 3 cut(s) 9, 441, 457
Fnu4HI GCNGC 3 cut(s) 270, 369, 400
FokI GGATG 2 cut(s) 547, 580
Fsp4HI GCNGC 3 cut(s) 270, 369, 400
FspBI CTAG 3 cut(s) 20, 152, 158
GluI GCNGC 3 cut(s) 270, 369, 400
HapII CCGG 1 cut(s) 584
Hin1II CATG 4 cut(s) 43, 71, 187, 409
HindIII AAGCTT 1 cut(s) 199
HpaII CCGG 1 cut(s) 584
HphI GGTGA 2 cut(s) 179, 467
Hpy188III TCNNGA 5 cut(s) 20, 91, 439, 461, 477
HpyCH4III ACNGT 3 cut(s) 28, 58, 241
HpyCH4V TGCA 3 cut(s) 296, 399, 543
HpyF10VI GCNNNNNNNGC 3 cut(s) 242, 396, 625
HpyF3I CTNAG 1 cut(s) 671
Hsp92II CATG 4 cut(s) 43, 71, 187, 409
Ksp22I TGATCA 3 cut(s) 9, 441, 457
Kzo9I GATC 5 cut(s) 9, 120, 423, 441, 457
LmnI GCTCC 2 cut(s) 368, 407
LpnPI CCDG 9 cut(s) 251, 446, 462, 517, 591, 597, 614, 624, 651
Lsp1109I GCAGC 3 cut(s) 281, 380, 411
LweI GCATC 4 cut(s) 418, 525, 552, 558
MaeI CTAG 3 cut(s) 20, 152, 158
MaeIII GTNAC 2 cut(s) 62, 327
MalI GATC 5 cut(s) 11, 122, 425, 443, 459
MboI GATC 5 cut(s) 9, 120, 423, 441, 457
MboII GAAGA 3 cut(s) 145, 298, 465
MfeI CAATTG 1 cut(s) 114
MluCI AATT 3 cut(s) 114, 246, 283
MnlI CCTC 5 cut(s) 56, 98, 112, 129, 314
MseI TTAA 1 cut(s) 353
MspI CCGG 1 cut(s) 584
MspR9I CCNGG 2 cut(s) 584, 639
MunI CAATTG 1 cut(s) 114
MvaI CCWGG 1 cut(s) 639
MwoI GCNNNNNNNGC 3 cut(s) 242, 396, 625
NciI CCSGG 1 cut(s) 584
NcoI CCATGG 1 cut(s) 405
NdeII GATC 5 cut(s) 9, 120, 423, 441, 457
NlaIII CATG 4 cut(s) 43, 71, 187, 409
NlaIV GGNNCC 3 cut(s) 36, 149, 364
NmuCI GTSAC 1 cut(s) 327
NspI RCATGY 1 cut(s) 187
PkrI GCNGC 3 cut(s) 271, 370, 401
Psp6I CCWGG 1 cut(s) 637
PspEI GGTNACC 1 cut(s) 62
PspGI CCWGG 1 cut(s) 637
PspN4I GGNNCC 3 cut(s) 36, 149, 364
PspPI GGNCC 2 cut(s) 34, 575
RsaI GTAC 1 cut(s) 109
RsaNI GTAC 1 cut(s) 108
SaqAI TTAA 1 cut(s) 353
SatI GCNGC 3 cut(s) 270, 369, 400
Sau3AI GATC 5 cut(s) 9, 120, 423, 441, 457
Sau96I GGNCC 2 cut(s) 34, 575
ScrFI CCNGG 2 cut(s) 584, 639
SetI ASST 9 cut(s) 90, 145, 159, 203, 274, 306, 320, 373, 404
SfaNI GCATC 4 cut(s) 418, 525, 552, 558
SfcI CTRYAG 1 cut(s) 289
SinI GGWCC 2 cut(s) 34, 575
SmlI CTYRAG 3 cut(s) 89, 202, 497
SmoI CTYRAG 3 cut(s) 89, 202, 497
Sse9I AATT 3 cut(s) 114, 246, 283
SsiI CCGC 1 cut(s) 366
SspMI CTAG 3 cut(s) 20, 152, 158
StyD4I CCNGG 2 cut(s) 582, 637
StyI CCWWGG 1 cut(s) 405
TaaI ACNGT 3 cut(s) 28, 58, 241
TaqI TCGA 1 cut(s) 426
TasI AATT 3 cut(s) 114, 246, 283
Tru1I TTAA 1 cut(s) 353
Tru9I TTAA 1 cut(s) 353
TseFI GTSAC 1 cut(s) 327
TseI GCWGC 3 cut(s) 269, 368, 399
Tsp45I GTSAC 1 cut(s) 327
VpaK11BI GGWCC 2 cut(s) 34, 575
XbaI TCTAGA 1 cut(s) 19
XceI RCATGY 1 cut(s) 187
XspI CTAG 3 cut(s) 20, 152, 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.